buffers

Summary

Summary: A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.

Top Publications

  1. pmc Cytoplasmic calcium buffers in intact human red cells
    T Tiffert
    The Physiological Laboratory, University of Cambridge, UK
    J Physiol 500:139-54. 1997
  2. pmc Buffer kinetics shape the spatiotemporal patterns of IP3-evoked Ca2+ signals
    Sheila L Dargan
    Department of Neurobiology and Behavior, University of California Irvine, CA 92697 4550, USA
    J Physiol 553:775-88. 2003
  3. ncbi Low-conductivity buffers for high-sensitivity NMR measurements
    Alexander E Kelly
    Graduate Group in Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
    J Am Chem Soc 124:12013-9. 2002
  4. ncbi Preparation of metal ion buffers for biological experimentation: a methods approach with emphasis on iron and zinc
    Amy G Aslamkhan
    Department of Physiology, University of Hawaii, Honolulu, Hawaii 96822, USA
    J Exp Zool 292:507-22. 2002
  5. ncbi In vitro-in vivo correlations for lipophilic, poorly water-soluble drugs
    J B Dressman
    Institute of Pharmaceutical Technology, J W Goethe University, Frankfurt am Main, Germany
    Eur J Pharm Sci 11:S73-80. 2000
  6. pmc Systemic buffers inhibit carcinogenesis in TRAMP mice
    Arig Ibrahim-Hashim
    Department of Radiology, H Lee Moffitt Cancer Center, Tampa, Florida 33612, USA
    J Urol 188:624-31. 2012
  7. ncbi A practical guide to the preparation of Ca(2+) buffers
    Donald M Bers
    Department of Pharmacology, University of California, Davis School of Medicine, Davis, California, USA
    Methods Cell Biol 99:1-26. 2010
  8. ncbi Iron autoxidation and free radical generation: effects of buffers, ligands, and chelators
    Kevin D Welch
    Biotechnology Center, Utah State University, Logan, Utah 84322 4705, USA
    Arch Biochem Biophys 397:360-9. 2002
  9. pmc Cytosolic Ca2+ buffers
    Beat Schwaller
    Unit of Anatomy, Department of Medicine, University of Fribourg, Route Albert Gockel 1, CH 1700 Fribourg, Switzerland
    Cold Spring Harb Perspect Biol 2:a004051. 2010
  10. ncbi Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer
    Mirco Castoldi
    European Molecular Biology Laboratory, Cell Biology and Biophysics Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Protein Expr Purif 32:83-8. 2003

Detail Information

Publications313 found, 100 shown here

  1. pmc Cytoplasmic calcium buffers in intact human red cells
    T Tiffert
    The Physiological Laboratory, University of Cambridge, UK
    J Physiol 500:139-54. 1997
    ..iv) The cell content of putative Ca2+ buffers with submicromolar Ca2+ dissociation constants was below the detection limit of the methods used here (less than ..
  2. pmc Buffer kinetics shape the spatiotemporal patterns of IP3-evoked Ca2+ signals
    Sheila L Dargan
    Department of Neurobiology and Behavior, University of California Irvine, CA 92697 4550, USA
    J Physiol 553:775-88. 2003
    ..and photoreleased IP3 in Xenopus oocytes to study how these properties are modulated by mobile cytosolic Ca2+ buffers. EGTA (a buffer with slow 'on-rate') speeded Ca2+ signals and 'balkanized' Ca2+ waves by dissociating them into ..
  3. ncbi Low-conductivity buffers for high-sensitivity NMR measurements
    Alexander E Kelly
    Graduate Group in Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
    J Am Chem Soc 124:12013-9. 2002
    ..We show that by choosing buffers with low ionic mobility, the sample conductivity can be dramatically reduced and the sensitivity substantially ..
  4. ncbi Preparation of metal ion buffers for biological experimentation: a methods approach with emphasis on iron and zinc
    Amy G Aslamkhan
    Department of Physiology, University of Hawaii, Honolulu, Hawaii 96822, USA
    J Exp Zool 292:507-22. 2002
    ..Selection of reagents appropriate for metal work (including water, salts, noncomplexing pH buffers) is briefly discussed...
  5. ncbi In vitro-in vivo correlations for lipophilic, poorly water-soluble drugs
    J B Dressman
    Institute of Pharmaceutical Technology, J W Goethe University, Frankfurt am Main, Germany
    Eur J Pharm Sci 11:S73-80. 2000
    ..fasted state effects can be predicted from dissolution data and, where several formulations were available for testing, dissolution tests could also be used to determine which would have the best in vivo performance...
  6. pmc Systemic buffers inhibit carcinogenesis in TRAMP mice
    Arig Ibrahim-Hashim
    Department of Radiology, H Lee Moffitt Cancer Center, Tampa, Florida 33612, USA
    J Urol 188:624-31. 2012
    ..We hypothesized that decreased acidosis in intraductal tumors would alter environmental selection pressures for acid adapted phenotypes and delay or prevent evolution to invasive cancer...
  7. ncbi A practical guide to the preparation of Ca(2+) buffers
    Donald M Bers
    Department of Pharmacology, University of California, Davis School of Medicine, Davis, California, USA
    Methods Cell Biol 99:1-26. 2010
    ..As with pH, it is often essential to use Ca(2+) buffers to control free [Ca(2+)] at the desired biologically relevant concentrations...
  8. ncbi Iron autoxidation and free radical generation: effects of buffers, ligands, and chelators
    Kevin D Welch
    Biotechnology Center, Utah State University, Logan, Utah 84322 4705, USA
    Arch Biochem Biophys 397:360-9. 2002
    ..Ferrous iron was shown to be able to directly reduce some quinones without the reduction of oxygen. In conclusion, this study demonstrates the complexity of iron chemistry, especially the chelation of iron and its subsequent reactivity...
  9. pmc Cytosolic Ca2+ buffers
    Beat Schwaller
    Unit of Anatomy, Department of Medicine, University of Fribourg, Route Albert Gockel 1, CH 1700 Fribourg, Switzerland
    Cold Spring Harb Perspect Biol 2:a004051. 2010
    Ca(2+) buffers," a class of cytosolic Ca(2+)-binding proteins, act as modulators of short-lived intracellular Ca(2+) signals; they affect both the temporal and spatial aspects of these transient increases in [Ca(2+)](i)...
  10. ncbi Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer
    Mirco Castoldi
    European Molecular Biology Laboratory, Cell Biology and Biophysics Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Protein Expr Purif 32:83-8. 2003
    ..The proposed protocol is rapid and yields more active tubulin than the traditional ion-exchange chromatography-based procedures...
  11. pmc The potential role of systemic buffers in reducing intratumoral extracellular pH and acid-mediated invasion
    Ariosto S Silva
    Laboratorio de Biologia Molecular, Centro Infantil Boldrini, Campinas, Sao Paulo, Brazil and Departments of Radiology and Integrative Mathematical Oncology, Moffitt Cancer Center, Tampa, Florida
    Cancer Res 69:2677-84. 2009
    ..Here, we investigate the hypothesis that increased systemic concentrations of pH buffers reduce intratumoral and peritumoral acidosis and, as a result, inhibit malignant growth...
  12. pmc Metabolic basis for low urine pH in type 2 diabetes
    Naim M Maalouf
    Charles and Jane Pak Center for Mineral Metabolism and Clinical Research and Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8885, USA
    Clin J Am Soc Nephrol 5:1277-81. 2010
    ..This study was conducted to assess the metabolic basis responsible for the excessive urinary acidity of individuals with type 2 diabetes...
  13. ncbi The continuing disappearance of "pure" Ca2+ buffers
    B Schwaller
    Unit of Anatomy, Department of Medicine, University of Fribourg, Route Albert Gockel 1, 1700, Fribourg, Switzerland
    Cell Mol Life Sci 66:275-300. 2009
    Advances in the understanding of a class of Ca(2+)-binding proteins usually referred to as "Ca(2+) buffers" are reported...
  14. ncbi Calcium buffering and protection from excitotoxic cell death by exogenous calbindin-D28k in HEK 293 cells
    G L Rintoul
    Department of Physiology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Cell Calcium 29:277-87. 2001
    ..These results provide direct evidence for a Ca(2+)-buffering effect of CaBP which serves to limit Ca(2+)entry and the depolarization of mitochondria, thereby protecting cells from death mediated most likely by apoptosis...
  15. pmc Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species
    João Loureiro
    Laboratory of Biotechnology and Cytomics, CESAM and Department of Biology, University of Aveiro, Campus Universitario de Santiago, 3810 193 Aveiro, Portugal
    Ann Bot 100:875-88. 2007
    ..plant sciences in the late 1980s and early 1990s, which was accompanied by development of many nuclear isolation buffers, only a few efforts were made to develop new buffer formulas...
  16. ncbi Intracellular magnesium and magnesium buffering
    Robert D Grubbs
    Department of Pharmacology and Toxicology, School of Medicine, Wright State University, Dayton, OH 45435, USA
    Biometals 15:251-9. 2002
    ..organelles will contribute to this regulation, it is binding of Mg2+ to an as yet poorly defined system of buffers that is largely responsible for determining the set point of this regulation...
  17. ncbi Hydrogen ion buffers for biological research
    N E Good
    Biochemistry 5:467-77. 1966
  18. pmc Thermal stability of DNA
    R D Blake
    Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, ME 04469 5735, USA
    Nucleic Acids Res 26:3323-32. 1998
    ..Statistical mechanical curves calculated with values of Tij([Na+]) , Delta Hij and these loop parameters are in good agreement with observation...
  19. ncbi Endogenous and exogenous Ca2+ buffers differentially modulate Ca2+-dependent inactivation of Ca(v)2.1 Ca2+ channels
    Lisa Kreiner
    Department of Pharmacology and Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 281:4691-8. 2006
    ..between Ca2+ current amplitude and inactivation in ways that were unexpected from behavior as passive Ca2+ buffers. We conclude that Ca2+-dependent inactivation of Ca(v)2...
  20. pmc Redox cycling compounds generate H2O2 in HTS buffers containing strong reducing reagents--real hits or promiscuous artifacts?
    Paul A Johnston
    University of Pittsburgh Drug Discovery Institute, Department of Pharmacology and Chemical Biology, Pittsburgh, PA 15260, USA
    Curr Opin Chem Biol 15:174-82. 2011
    ..In an attempt to mitigate the serious impact of RCCs on probe and lead generation, two groups have independently developed assays to indentify RCCs...
  21. pmc Comparison of four nuclear isolation buffers for plant DNA flow cytometry
    João Loureiro
    Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitario de Santiago, 3810 193 Aveiro, Portugal
    Ann Bot 98:679-89. 2006
    ..Although nuclear isolation buffers differ considerably in chemical composition, no systematic comparison of their performance has been made until ..
  22. ncbi Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives
    Changsheng Sun
    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    Protein Expr Purif 82:226-31. 2012
    ..accepted that the high concentrations of salts or other special agents in most protein affinity chromatography buffers can affect enzyme activity, including that of TEV protease...
  23. ncbi Amyloid-beta aggregates formed at polar-nonpolar interfaces differ from amyloid-beta protofibrils produced in aqueous buffers
    Michael R Nichols
    Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida 32224, USA
    Microsc Res Tech 67:164-74. 2005
    ..Abeta protofibrils, prepared by vigorous overnight agitation of monomeric Abeta(1-40) in low ionic strength buffers. These aggregates were quite stable and disaggregated to only a limited extent on dilution...
  24. ncbi Regulation of exocytosis in neuroendocrine cells: spatial organization of channels and vesicles, stimulus-secretion coupling, calcium buffers and modulation
    K S Kits
    Department of Neurophysiology, Research Institute for Neurosciences, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands
    Brain Res Brain Res Rev 33:78-94. 2000
    ..This distinctive organization relates to the sensitivity of the release process to mobile calcium buffers, the resulting relationship between calcium influx and release and the modulatory mechanisms regulating the ..
  25. ncbi Mitochondrial regulation of intracellular Ca2+ signaling: more than just simple Ca2+ buffers
    Anant B Parekh
    Department of Physiology, University of Oxford, Oxford OX1 3PT, UK
    News Physiol Sci 18:252-6. 2003
    ....
  26. pmc Calcium domains around single and clustered IP3 receptors and their modulation by buffers
    S Rudiger
    Institute of Physics, Humboldt Universitat zu Berlin, Berlin, Germany
    Biophys J 99:3-12. 2010
    ..While immobile buffer affects transient levels only, mobile buffers in general reduce both transient and stationary domains, giving rise to Ca(2+) evacuation and biphasic modulation ..
  27. pmc Involvement of a cytosine side chain in proton transfer in the rate-determining step of ribozyme self-cleavage
    I H Shih
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 98:1489-94. 2001
    ..Together, these data provide strong support for a mechanism where an RNA side chain participates directly in general acid or general base catalysis of the wild-type ribozyme to facilitate RNA cleavage...
  28. ncbi Calcium dynamics, buffering, and buffer saturation in the boutons of dentate granule-cell axons in the hilus
    Meyer B Jackson
    Division of Neuroscience, John Curtin School of Medical Research, Canberra, ACT 0200, Australia
    J Neurosci 23:1612-21. 2003
    ..Buffer saturation may also be relevant to the presynaptic induction of long-term potentiation at synapses formed by dentate granule cells...
  29. ncbi Determination of baseline human nasal pH and the effect of intranasally administered buffers
    N Washington
    Department of Surgery, School of Medical and Surgical Sciences, University of Nottingham, E Floor, West Block, Queen s Medical Centre, Nottingham, UK
    Int J Pharm 198:139-46. 2000
    ..and hence this study was performed to investigate normal pH values and whether pH could be manipulated by various buffers. Twelve healthy volunteers participated in a study to measure pH in the anterior and posterior sites of the nasal ..
  30. pmc Oxidation artifacts in the electrospray mass spectrometry of Abeta Peptide
    Maolian Chen
    Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996 1600
    Anal Chem 79:2031-6. 2007
    ..The resultant mass shift and reduced sensitivity can adversely affect H/D exchange experiments. These effects can be avoided by adding a redox buffer or (preferably) by repolishing the emitter, especially to a rounded geometry...
  31. ncbi The effect of aminosulfonate buffers on the light responses and intracellular pH of goldfish retinal horizontal cells
    Stuart Trenholm
    Neuroscience Institute, Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Neurochem 115:102-11. 2010
    ..Most evidence supporting the proton model has depended on the effect of proton buffers, in particular aminosulfonates, but these agents could potentially have effects other than external pH regulation...
  32. ncbi Cardiopulmonary events during hemodialysis: effects of dialysis membranes and dialysate buffers
    M A Munger
    Department of Pharmacy Practice, Division of Nephrology and Hypertension, School of Medicine, University of Utah, Salt Lake City, UT 84112, USA
    Am J Kidney Dis 36:130-9. 2000
    ..We conclude that cardiopulmonary events can occur frequently during hemodialysis, and the frequency is dependent on the type of dialysis membrane and dialysate buffer used...
  33. ncbi Traceless Staudinger acetylation of azides in aqueous buffers
    Sylwia Sowa
    Maria Curie Sklodowska University, Department of Organic Chemistry, ul Gliniana 33, 20 614 Lublin, Poland
    Bioorg Med Chem 21:3465-72. 2013
    ..Additionally, we present an improved synthesis pathway for the water-soluble phosphinothiol linkers requiring less steps in a comparable overall yield in comparison to previously published protocols...
  34. ncbi Influence of organic buffers on bacteriocin production by Streptococcus thermophilus ST110
    George A Somkuti
    Agricultural Research Service, US Department of Agriculture, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Curr Microbiol 55:173-7. 2007
    ..The results showed that whey permeate-based media may be suitable for producing large amounts of thermophilin 110 needed for controlling spoilage pediococci in industrial wine and beer fermentations...
  35. pmc Structure and assembly of a T=1 virus-like particle in BK polyomavirus
    Josefina Nilsson
    Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    J Virol 79:5337-45. 2005
    ..The meeting densities seen at the threefold axes in the T=1 particle were consistent with the triple-helix interlinking contact at the local threefold axes in the T=7 structure...
  36. ncbi Spectroscopic evidence that osmolytes used in crystallization buffers inhibit a conformation change in a membrane protein
    Gail E Fanucci
    Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, Virginia 22904, USA
    Biochemistry 42:13106-12. 2003
    ..Specifically, if SDSL is performed with the buffers used for the crystallization of BtuB, the substrate-induced order-disorder transition of the N-terminal Ton box ..
  37. ncbi Low-resource method for extracting the malarial biomarker histidine-rich protein II to enhance diagnostic test performance
    Keersten M Davis
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235 1822, United States
    Anal Chem 84:6136-42. 2012
    ..Sample preprocessing improved the visual limit of detection of this test by over 8-fold, suggesting that the combination of both low-resource technologies could prove to be useful in malaria eradication efforts...
  38. ncbi A nondestructive molecule extraction method allowing morphological and molecular analyses using a single tissue section
    Wei Sing Chu
    Department of Scientific Laboratories, Armed Forces Institute of Pathology, Washington, DC 20306 6000, USA
    Lab Invest 85:1416-28. 2005
    ..Thus, the NDME method allows, for the first time, both histological diagnosis and molecular analysis on a single tissue section, whether it is from frozen or fixed tissue specimens...
  39. ncbi Membrane associated Ca2+ buffers in the heart
    Dukgyu Lee
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada T6G2H7
    BMB Rep 43:151-7. 2010
    ..The Ca2+ homeostasis regulated by the endoplasmic and sarcoplasmic reticulum is critical for the development and proper function of the heart...
  40. ncbi Evaluation of extraction buffers using the current approach of detecting multiple allergenic and nonallergenic proteins in food
    Carmen D Westphal
    US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, MD 20708, USA
    J AOAC Int 87:1458-65. 2004
    ..In this study, differences in extractability of 3 allergenic foods were evaluated by using 4 different extraction buffers. The conclusion is that optimum allergen extractability was buffer-dependent, and no single buffer is appropriate ..
  41. ncbi Effects of pH control with phthalate buffers on hot-water extraction of hemicelluloses from spruce wood
    Tao Song
    Laboratory of Wood and Paper Chemistry, Process Chemistry Centre, Abo Akademi University, Porthansgatan 3, FI 20500 Turku Abo, Finland
    Bioresour Technol 102:10518-23. 2011
    ..8, 4.0, 4.2 and 4.4) achieved by using phthalate buffers. Static batch extractions were carried out in an accelerated solvent extractor (ASE-300)...
  42. pmc Bench-to-bedside review: treating acid-base abnormalities in the intensive care unit - the role of buffers
    Brian K Gehlbach
    Section of Pulmonary and Critical Care, University of Chicago, Chicago, Illinois, USA
    Crit Care 8:259-65. 2004
    ..The evidence supporting and refuting attempts to correct arterial pH through the administration of currently available buffers is presented.
  43. pmc Evolution of cardiac calcium waves from stochastic calcium sparks
    L T Izu
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Biophys J 80:103-20. 2001
    ..This allows Ca2+ waves to form and propagate given a sufficiently large initiation region, but prevents a single spark or a small group of sparks from triggering a wave...
  44. ncbi Modulation of neuronal excitability by intracellular calcium buffering: from spiking to bursting
    C Roussel
    Laboratoire de Neurophysiologie, Faculte de Medecine, Universite Libre de Bruxelles, Route de Lennik 808, B 1070 Bruxelles, Belgium
    Cell Calcium 39:455-66. 2006
    ....
  45. ncbi Ferritin iron mineralization proceeds by different mechanisms in MOPS and imidazole buffers
    Claine L Snow
    Department of Chemistry and Biochemistry, C 210 Benson Building, Brigham Young University, Provo, UT 84602, United States
    J Inorg Biochem 105:972-7. 2011
    ....
  46. ncbi Saliva and dental caries
    M Lenander-Lumikari
    Department of Cariology and Turku Immunology Centre, Institute of Dentistry, University of Turku, Finland
    Adv Dent Res 14:40-7. 2000
    ..In this article, our aim is to focus on the effects of saliva and salivary constituents on cariogenic bacteria and the subsequent development of dental caries...
  47. ncbi High-activity biocatalysts in organic media: solid-state buffers as the immobilisation matrix for protein-coated microcrystals
    Michaela Kreiner
    Department of Chemistry, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, Scotland, UK
    Biotechnol Bioeng 87:24-33. 2004
    ..a simple inorganic salt, K(2)SO(4), to other compounds, both inorganic and zwitterionic, that act as solid-state buffers for biocatalysis in organic media...
  48. pmc Diffusion barriers limit the effect of mobile calcium buffers on exocytosis of large dense cored vesicles
    K S Kits
    Membrane Physiology Section, Research Institute Neurosciences, Vrije Universiteit Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The
    Biophys J 76:1693-705. 1999
    ..cells, activated by calcium entry through voltage-gated calcium channels, is very sensitive to mobile calcium buffers (complete block at 800 microM ethylene glycol bis(beta-aminoethyl ether)-N,N,N'N'-tetraacetic acid (EGTA))...
  49. ncbi A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis
    A R Thornhill
    Guy s and St Thomas Centre for Preimplantation Genetic Diagnosis, London SE1 7EH, UK
    Prenat Diagn 21:490-7. 2001
    ..A number of different strategies (including the use of lysis buffers to break down the cell and make the DNA accessible) have been employed to combat ADO with varying degrees of ..
  50. ncbi Copper(II) complexation properties and surfactant activity of 3-[N, N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid and N-(2-Hydroxyethyl)piperazine-N'-2-hydroxypropanesulfonic acid pH buffers which may affect trace metal speciation in in vitro
    M T Vasconcelos
    Departamento de Quimica, Faculdade de Ciencias, Universidade do Porto, Rua do Campo Alegre 687, Porto, P4150, Portugal
    Anal Biochem 265:193-201. 1998
    Disadvantages of the some zwitterionic pH buffers are (i) that they can interact with metal ions as well as protons, and (ii) that they may have a surfactant effect in chemical or in vitro biological or biochemical studies...
  51. ncbi Acid-extrusion from tissue: the interplay between membrane transporters and pH buffers
    Alzbeta Hulikova
    Department of Physiology, Anatomy and Genetics, Oxford, UK
    Curr Pharm Des 18:1331-7. 2012
    ..The major targets include H⁺ pumps, Na⁺/H⁺ exchangers and carbonic anhydrases. The effectiveness of such therapy will depend on the correct identification of rate-limiting steps in pHi regulation in a specific type of cancer...
  52. ncbi Effects of calcium buffers and calbindin-D28k upon histamine-induced calcium oscillations and calcium waves in HeLa cells
    Gordon L Rintoul
    Department of Physiology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
    Cell Calcium 34:131-44. 2003
    The effects of the artificial Ca(2+) buffers EGTA and BAPTA upon histamine-induced Ca(2+) oscillations and calcium waves were studied in HeLa cells...
  53. ncbi Glutarate and N-acetyl-L-glutamate buffers for cell-free synthesis of selectively 15N-labelled proteins
    Xinying Jia
    Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia
    J Biomol NMR 44:59-67. 2009
    ..While the best yields are usually obtained with buffers containing high concentrations of potassium L-glutamate, preparation of selectively 15N-Glu labelled samples ..
  54. ncbi Investigation of a novel mixed-mode stationary phase for capillary electrochromatography. Part III: Separation of nucleosides and nucleic acid bases on sulfonated naphthalimido-modified silyl silica gel
    Kaname Ohyama
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan
    J Sep Sci 28:767-73. 2005
    ..In addition, when a double stepwise gradient was employed to accelerate the latest peak (adenine), the elution time was shortened to less than half its original duration...
  55. ncbi Measurement of dissociation constants (pKa values) of organic compounds by multiplexed capillary electrophoresis using aqueous and cosolvent buffers
    Marina Shalaeva
    Pfizer Global Research and Development, Groton Laboratories, Groton, Connecticut 06340, USA
    J Pharm Sci 97:2581-606. 2008
    ..It has been demonstrated that the method allows direct pK(a) measurements in aqueous buffers for many compounds of low solubility, often unattainable by other methods...
  56. pmc Biological pH buffers in IVF: help or hindrance to success
    Matthew A Will
    Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48108, USA
    J Assist Reprod Genet 28:711-24. 2011
    ..One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH...
  57. ncbi Evaluation of different buffers on plasmid DNA encapsulation into PLGA microparticles
    Man Tsuey Tse
    Centre for Drug Delivery Research, The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N 1AX, United Kingdom
    Int J Pharm 370:33-40. 2009
    ..This study compares the use of two buffers, 0.1M NaHCO(3) and 0...
  58. ncbi The concept of "buffering" in systems and control theory: from metaphor to math
    Bernhard M Schmitt
    Department of Anatomy I, University of Wurzburg, Koellikerstrasse 6, 97070 Wurzburg, Germany
    Chembiochem 5:1384-92. 2004
    ....
  59. ncbi Accumulation of succinimide in a recombinant monoclonal antibody in mildly acidic buffers under elevated temperatures
    Grace C Chu
    Department of Pharmaceutics, Amgen, Inc, One Amgen Center Drive, MS 8 1 C, Thousand Oaks, California 91320, USA
    Pharm Res 24:1145-56. 2007
    The purpose of this paper was to identify the location of a succinimide and determine the rate of its formation and hydrolysis in a recombinant human monoclonal IgG2 antibody aged in mildly acidic buffers at elevated temperatures.
  60. ncbi Taurine buffers glutamate homeostasis in retinal cells in vitro under hypoxic conditions
    Fang Chen
    Department of Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Third Military Medical University, Chongqing, China
    Ophthalmic Res 44:105-12. 2010
    ..We investigated whether taurine indirectly protects neurons under hypoxia by affecting retinal Müller cells, which are known to play important roles in the regulation of retinal glutamate content...
  61. ncbi Use of biocompatible buffers to reduce the concentration overpotential for hydrogen evolution
    Adriaan W Jeremiasse
    Wetsus, Centre of Excellence for Sustainable Water Technology, Agora 1, P O Box 1113, 8900 CC Leeuwarden, The Netherlands
    Environ Sci Technol 43:6882-7. 2009
    ..Thus, to reduce the HER overpotential of an MEC, buffer (i.e., pKa), buffer concentration, linear flow speed, and current density must be well balanced with the expected operational pH...
  62. ncbi Simian virus 40 VP1 capsid protein forms polymorphic assemblies in vitro
    Shin nosuke Kanesashi
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8503, Japan
    J Gen Virol 84:1899-905. 2003
    ..VP1 is thus quite unique in that it can assemble into such diverse structures. These observations provide clues that will help elucidate the mechanisms underlying SV40 capsid formation...
  63. pmc Extra activation component of calcium release in frog muscle fibres
    Paul C Pape
    Département de Physiologie et Biophysique, Université de Sherbrooke Faculté de médicine, 3001, 12e Avenue Nord, Sherbrooke Quebec, Canada J1H 5N4
    J Physiol 542:867-86. 2002
    ..The enhanced release probably involves CICR and appears to involve another positive feedback mechanism in which Ca(2+) release speeds up the activation of voltage sensors...
  64. pmc Distinct properties of CRAC and MIC channels in RBL cells
    J Ashot Kozak
    Department of Physiology and Biophysics, University of California at Irvine, Irvine, CA 92697, USA
    J Gen Physiol 120:221-35. 2002
    ..The biophysical characteristics of MIC current match well with cloned and expressed TRPM7 channels. Previous results are reevaluated in terms of separate CRAC and MIC channels...
  65. pmc Transmitter release modulation by intracellular Ca2+ buffers in facilitating and depressing nerve terminals of pyramidal cells in layer 2/3 of the rat neocortex indicates a target cell-specific difference in presynaptic calcium dynamics
    A Rozov
    Abteilung Zellphysiologie, Max-Planck-Institut fur medizinische Forschung, Jahnstrasse 29, D-69120 Heidelberg, Germany
    J Physiol 531:807-26. 2001
    ..3. Intracellular loading of pyramidal neurones with the fast and slowly acting Ca2+ buffers BAPTA and EGTA differentially reduced transmitter release in these two types of terminal...
  66. pmc Recruitment of Ca(2+) release channels by calcium-induced Ca(2+) release does not appear to occur in isolated Ca(2+) release sites in frog skeletal muscle
    Karine Fenelon
    Département de Physiologie et Biophysique, Université de Sherbrooke Faculté de médicine, 3001, 12e Avenue Nord, Sherbrooke Quebec, Canada J1H 5N4
    J Physiol 544:777-91. 2002
    ..Since BAPTA never decreased f(Ca), the results indicate that neighbouring channels are not activated by CICR with small depolarisations when [Ca(SR)] is 300-3000 microM...
  67. ncbi Hydroquinone as a buffer additive for suppression of bubbles formed by electrochemical oxidation of the CE buffer at the outlet electrode in capillary electrophoresis/electrospray ionization-mass spectrometry
    M Moini
    Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA
    Anal Chem 71:1658-61. 1999
    ....
  68. ncbi Microelectronic array devices and techniques for electric field enhanced DNA hybridization in low-conductance buffers
    Christian Gurtner
    Nanogen Inc, San Diego, CA 92121, USA
    Electrophoresis 23:1543-50. 2002
    ..With regard to the planar microelectronic array devices, special low-conductance buffers were developed in order to maintain rapid transport of DNA molecules and to facilitate hybridization within the ..
  69. ncbi Peritoneal dialysis fluids with a physiologic pH based on either lactate or bicarbonate buffer-effects on human mesothelial cells
    J Plum
    Department of Nephrology and Rheumatology, Heinrich Heine University, Dusseldorf, Germany
    Am J Kidney Dis 38:867-75. 2001
    ..However, distinct parameters of cell metabolism were superior with Bic compared with Lac. Especially Glupoly was inferior in combination with Lac as a buffer...
  70. ncbi The effects of training intensity on muscle buffer capacity in females
    Johann Edge
    Team Sport Research Group, School of Human Movement and Exercise Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia
    Eur J Appl Physiol 96:97-105. 2006
    ..This suggests that training intensity is an important determinant of changes to beta m(in vitro)...
  71. pmc Calculation of the equilibrium pH in a multiple-buffered aqueous solution based on partitioning of proton buffering: a new predictive formula
    Minhtri K Nguyen
    Division of Nephrology, David Geffen School of Medicine at UCLA, 10833 Le Conte Ave, Rm 7 155 Factor Bldg, Los Angeles, CA 90095, USA
    Am J Physiol Renal Physiol 296:F1521-9. 2009
    Upon the addition of protons to an aqueous solution containing multiple buffers, the final H+ concentration ([H+]) at equilibrium is determined by the partitioning of added H+ among the various buffer components...
  72. ncbi Evaluation of the buffering capacity of powdered cow's, goat's and soy milk and non-prescription antacids in the treatment of non-ulcer dyspepsia
    D Lutchman
    School of Pharmacy and Pharmacology, University of KwaZulu Natal, Durban, South Africa
    S Afr Med J 96:57-61. 2006
    ..Treatment choice commonly includes over the counter medicines and home remedies, e.g. milk...
  73. ncbi Rapid detection of Clostridium botulinum toxins A, B, E, and F in clinical samples, selected food matrices, and buffer using paramagnetic bead-based electrochemiluminescence detection
    Victor R Rivera
    U S Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA
    Anal Biochem 353:248-56. 2006
    ..These rapid sensitive assays may have potential use in clinical settings, research studies, and screening of food products for botulinum toxins...
  74. ncbi Pain perception after subcutaneous injections of media containing different buffers
    Torben Laursen
    Medical Department M Diabetes and Endocrinology, Aarhus Sygehus, NBG, Aarhus University Hospital, Aarhus, Denmark
    Basic Clin Pharmacol Toxicol 98:218-21. 2006
    ..after injection into the thigh of three formulations, which differed with respect to pH and buffers (histidine, citrate and saline, respectively)...
  75. pmc The structure of DNA overstretched from the 5'5' ends differs from the structure of DNA overstretched from the 3'3' ends
    Claudia Danilowicz
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:13196-201. 2009
    ..These differences and additional findings are well-accommodated by the corresponding structures predicted on theoretical grounds...
  76. ncbi Postcoital testing after the use of a bio-adhesive acid buffering gel (ACIDFORM) and a 2% nonoxynol-9 product
    Eliana Amaral
    Department of Obstetrics and Gynecology, UNICAMP, Caixa Postal 6181, Campinas, SP CEP 13 084 971, Brazil
    Contraception 70:492-7. 2004
    ..05, Wilcoxon test). All treated cycles had five or fewer progressively motile sperm in midcycle cervical mucus, confirming in vivo the spermicidal effect of ACIDFORM previously observed in vitro and in animal models...
  77. pmc Intrinsic H(+) ion mobility in the rabbit ventricular myocyte
    R D Vaughan-Jones
    University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK
    J Physiol 541:139-58. 2002
    ..of intracellular H(+) is discussed in terms of a diffusive shuttling of H(+) equivalents on high capacity mobile buffers which comprise about half (approximately 11 mM) of the total intrinsic buffering capacity within the myocyte (the ..
  78. ncbi Presynaptic Ca2+ buffers control the strength of a fast post-tetanic hyperpolarization mediated by the alpha3 Na(+)/K(+)-ATPase
    Jun Hee Kim
    The Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nat Neurosci 10:196-205. 2007
    ..Notably, increasing the concentration of internal Ca(2+) buffers or decreasing Ca(2+) influx led to larger PTH amplitudes, indicating that an increase in [Ca(2+)](i) regulates ..
  79. ncbi Determination of impurities in heparin by capillary electrophoresis using high molarity phosphate buffers
    Todd Wielgos
    Baxter Healthcare Corporation, Round Lake, IL 60073, USA
    J Pharm Biomed Anal 49:319-26. 2009
    ..A high speed, high resolution version of the method was developed using 600mM lithium phosphate, pH 2.8 in a 21.5cm x 25microm i.d. capillary which provided limits of detection for OSCS that were below 0.1%...
  80. ncbi Cardiac-directed parvalbumin transgene expression in mice shows marked heart rate dependence of delayed Ca2+ buffering action
    Sharlene M Day
    Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0644, USA
    Physiol Genomics 33:312-22. 2008
    ..refining the dose and frequency-response relationships of Parv in the heart or engineering novel Parv-based Ca2+ buffers with modified Mg2+ and Ca2+ affinities to limit systolic Ca2+ buffering may hold promise for the development of ..
  81. ncbi Salivary proteins: protective and diagnostic value in cariology?
    A van Nieuw Amerongen
    Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, Amsterdam, The Netherlands a van_nieuw_
    Caries Res 38:247-53. 2004
    ..These are potentially suited as templates for the design of a new generation of antibiotics, since they kill a broad spectrum of microorganisms, while hardly evoking resistance, in contrast to the classical antibiotics...
  82. ncbi Infection-induced lung injury is worsened after renal buffering of hypercapnic acidosis
    Alistair D Nichol
    School of Medicine and Medical, Conway Institute, University, College Dublin, Ireland
    Crit Care Med 37:2953-61. 2009
    ..We therefore wished to determine the effects of buffered hypercapnia on acute lung injury induced by endotoxin or Escherichia coli infection in vivo...
  83. pmc Approximate analytical time-dependent solutions to describe large-amplitude local calcium transients in the presence of buffers
    Lidia A Mironova
    DFG Center of Molecular Physiology of the Brain, Department of Neuro and Sensory Physiology, Georg August University, Gottingen, Germany
    Biophys J 94:349-58. 2008
    ..These nanodomains are formed due to combined effects of Ca(2+) diffusion and binding to the cytoplasmic buffers. In this article we derived simple analytical expressions to describe Ca(2+) diffusion in the presence of mobile ..
  84. pmc Modeling of the modulation by buffers of Ca2+ release through clusters of IP3 receptors
    S Zeller
    Institute for Theoretical Physics, Technische Universitat Berlin, Berlin, Germany
    Biophys J 97:992-1002. 2009
    ..It is modeled here by reaction-diffusion equations for the free Ca(2+) and Ca(2+) buffers, with spatially discrete clusters of stochastic IP(3) receptor channels (IP(3)Rs) controlling the release of Ca(2+..
  85. ncbi Presynaptic Ca2+ dynamics, Ca2+ buffers and synaptic efficacy
    Nail Burnashev
    Department of Experimental Neurophysiology, Faculty of Earth and Life Sciences, CNCR, Vrije Universiteit Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    Cell Calcium 37:489-95. 2005
    ..diffusional distance between the source of Ca2+ and the Ca2+ sensor and Ca2+ buffering by endogenous Ca2+ buffers. In many synapses transmitter release can be enhanced (facilitated) during repetitive activity of neurons...
  86. ncbi Total weak acid concentration and effective dissociation constant of nonvolatile buffers in human plasma
    P D Constable
    Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61802, USA
    J Appl Physiol (1985) 91:1364-71. 2001
    ..The approach requires species-specific values for the total concentration of plasma nonvolatile buffers (A(tot)) and the effective dissociation constant for plasma nonvolatile buffers (K(a)), but these values have not ..
  87. ncbi Investigations into the stabilization of drugs by sugar glasses: III. The influence of various high-pH buffers
    Jonas H C Eriksson
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, 9713 AV Groningen, The Netherlands
    Pharm Res 20:1437-43. 2003
    To study the effect of the high-pH buffers ammediol, borax, CHES, TRIS, and Tricine on the glass transition temperature of the freeze concentrated fraction (Tg') of trehalose/buffer and inulin/buffer solutions at pH 6.0 and pH 9.8...
  88. ncbi The important role of taurine in oxidative metabolism
    Svend Høime Hansen
    Department of Clinical Biochemistry 3 01 3, Rigshospitalet, Copenhagen University Hospital, DK 2100 Copenhagen, Denmark
    Adv Exp Med Biol 583:129-35. 2006
    ..Consequently, the model represents a new concept for understanding mitochondrial dysfunction by emphasising the importance of taurine for providing sufficient pH buffering in the mitochondrial matrix...
  89. ncbi Sample solublization buffers for two-dimensional electrophoresis
    Walter Weiss
    Technical University of Munich, Munich, Germany
    Methods Mol Biol 424:35-42. 2008
    ..In this chapter, the major constituents of sample solubilization/lysis buffers will be briefly reviewed, some general sample preparation guidelines will be given, and the most common protein ..
  90. pmc Saltatory and continuous calcium waves and the rapid buffering approximation
    Damián E Strier
    Departamento de Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
    Biophys J 85:3575-86. 2003
    ..However, most models of calcium waves either treat buffers phenomenologically or assume that they are in equilibrium with calcium (the rapid buffering approximation)...
  91. ncbi The thermodynamics of the partitioning of ionizing molecules between aqueous buffers and phospholipid membranes
    Rupert P Austin
    Department of Physical and Metabolic Sciences, AstraZeneca R and D Charnwood, Bakewell Road, Loughborough, Leicestershire, LE11 5RH, UK
    Pharm Res 22:1649-57. 2005
    To study the thermodynamics of partitioning of eight ionising dual D2-recepto beta2-adrenoceptor agonists between vesicles of L-alpha-dimyristoylphosphatidylcholine (DMPC) and aqueous buffers.
  92. ncbi Enhancement of in vitro hair shaft elongation in follicles stored in buffers that prevent follicle cell apoptosis
    Walter Krugluger
    Moser Medical Group, Clinic Vienna, Vienna, Austria
    Dermatol Surg 30:1-5; discussion 5. 2004
    ..In this study, we investigated the effect of different storage solutions and inhibitors of apoptotic cell death (ACD) on hair follicle cell viability by measuring in vitro hair shaft elongation (HSE) for 5 days...
  93. pmc Luminal Ca(2+) content regulates intracellular Ca(2+) release in subepicardial myocytes of intact beating mouse hearts: effect of exogenous buffers
    Dmytro Kornyeyev
    School of Engineering, Univ of California Merced, 5200 N Lake Rd, Merced, CA 95343, USA
    Am J Physiol Heart Circ Physiol 298:H2138-53. 2010
    ..Thus, intra-SR Ca(2+) dynamics set the refractoriness and frequency dependence of Ca(2+) transients in subepicardial ventricular myocytes...
  94. pmc Modeling Ca2+ feedback on a single inositol 1,4,5-trisphosphate receptor and its modulation by Ca2+ buffers
    Jianwei Shuai
    Department of Physics, Xiamen University, Xiamen, China
    Biophys J 95:3738-52. 2008
    ..A major conclusion is that self-activation depends crucially on stationary cytosolic Ca(2+) buffers that slow the collapse of the local [Ca(2+)] microdomain after closure...
  95. ncbi Experimental determination of net protein charge and A(tot) and K(a) of nonvolatile buffers in canine plasma
    Peter D Constable
    Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA
    J Vet Intern Med 19:507-14. 2005
    ..simplified strong ion approach, which requires accurate values for the total concentration of plasma nonvolatile buffers (A(tot)) and the effective dissociation constant for plasma weak acids (K(a))...
  96. pmc Proton transfer dynamics at the membrane/water interface: dependence on the fixed and mobile pH buffers, on the size and form of membrane particles, and on the interfacial potential barrier
    Dmitry A Cherepanov
    Division of Biophysics, Faculty of Biology Chemistry, University of Osnabruck, D 49069 Osnabruck, Germany
    Biophys J 86:665-80. 2004
    ..the system of diffusion equations we analyzed how the proton relaxation depends on the concentration of mobile pH buffers, on the surface buffer capacity, on the form and size of membrane particles, and on the height of the potential ..
  97. ncbi Improved acidosis correction and recovery of mesothelial cell mass with neutral-pH bicarbonate dialysis solution among children undergoing automated peritoneal dialysis
    Susanne Haas
    Children s Hospital, University of Heidelberg, Heidelberg, Germany
    J Am Soc Nephrol 14:2632-8. 2003
    ..Among children undergoing APD, neutral-pH, bicarbonate-buffered PD fluid provides more effective correction of metabolic acidosis and better preservation of peritoneal cell mass than do conventional, acidic, lactate-based fluids...
  98. pmc Combining voltage and calcium imaging from neuronal dendrites
    Marco Canepari
    Biozentrum, Department of Pharmacology and Neurobiology, University of Basel, Basel, Switzerland
    Cell Mol Neurobiol 28:1079-93. 2008
    ..Using these two examples, the method is explained in detail...
  99. ncbi DNA and buffers: are there any noninteracting, neutral pH buffers?
    N C Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Anal Biochem 287:167-75. 2000
    The interaction of DNA with various neutral pH, amine-based buffers has been analyzed by free solution capillary electrophoresis, using a mixture of a plasmid-sized DNA molecule and a small DNA oligonucleotide as the reporter system...
  100. ncbi Dissolution media simulating conditions in the proximal human gastrointestinal tract: an update
    Ekarat Jantratid
    Institute of Pharmaceutical Technology, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    Pharm Res 25:1663-76. 2008
    ....
  101. ncbi Influence of alkaline buffers on cytoplasmic pH in myocardial cells exposed to metabolic acidosis
    Y C Li
    Department of Anaesthesiology, Uppsala University Hospital, Sweden
    Resuscitation 32:33-44. 1996
    The influence of different clinically used alkaline buffers on cytoplasmic pH in normal as well as acidotic rat myocardial cells was investigated in this study by means of the fluorescent intracellular probe 2',7'-bis-(carboxyethyl)-5,6-..

Research Grants65

  1. Insulin Signaling and Metabolic Effects through CLK2 Kinase
    Pere Puigserver; Fiscal Year: 2013
    ..The liver is one of the key tissues that depending of the physio/pathological conditions buffers whole body nutrient homeostasis...
  2. Development and testing of polyvalent anthrax toxin inhibitors
    JEREMY S MOGRIDGE; Fiscal Year: 2012
    ..Inhibitor solubility will be assessed in buffers made within a clinically suitable range of pH values, and in the presence of co- solvents and surfactants...
  3. Trafficking of the EGF receptor to the nucleus: Mechanisms and Effects
    Michael H Nathanson; Fiscal Year: 2013
    ..2. Whether and how EGF increases Ca2+ in the nucleus will be determined. Targeted InsP3 buffers will be used to determine whether EGF, like HGF, specifically induces InsP3 formation within the nucleus...
  4. A fully integrated assay and platform for detecting Clostridium difficile
    BRUCE D IRVINE; Fiscal Year: 2010
    ..sample processing methods selected for this plan are robust and simple, avoiding the need for wash steps, elution buffers, or centrifugation...
  5. Linear quadrupole/linear ion trap LC/MS/MS mass spectrometer
    Rodney C Baker; Fiscal Year: 2010
    ..Coupled with the appropriate buffers, liquid chromatography desalts the sample, again increasing sensitivity...
  6. Eosinophil Peroxidase in Allergic Inflammation
    Arne Slungaard; Fiscal Year: 2012
    ..We hypothesize that SCN- "buffers" against generation by EPO of the more cytotoxic NO2-- and Br-- based oxidants and consequently serum SCN- levels,..
  7. Traffic and Respiratory Health
    Brian P Leaderer; Fiscal Year: 2013
    ..Our goal is to estimate levels of pollution due to traffic within buffers (e.g. a 500 m radius) around a residence within a neighborhood, i.e., residential-scale pollution levels...
  8. Assessing the Influence of Oxytocin on Postpartum Depression Using Brain Imaging
    Julia R Heiman; Fiscal Year: 2010
    ..understanding of neural activation patterns characteristic of the postpartum period and explore whether oxytocin buffers women against depression by inhibiting neural activation to negatively arousing stimuli in limbic brain regions ..
  9. Stress Responses, Psychosocial Stress and Pain Perception in African Americans
    Susan S Girdler; Fiscal Year: 2010
    ..e., discrimination and difficulties), and recent negative life events. Also, potential stress buffers (e.g. religiosity, social support) will be assessed...
  10. (PQA4) GPS exposure to environments &relations with biomarkers of cancer risk
    Jacqueline Kerr; Fiscal Year: 2013
    ..g. parcel size. Static residential GIS buffers of access to neighborhood resources will be created within a 1km street network buffer around a participant'..
  11. Direct RT-PCR detection of RNA pathogens and mRNA expression in crude samples
    Zhian Zhang; Fiscal Year: 2010
    ..The method also includes development and optimization of buffers that are compatible with both the RT and DNA polymerase activities, as well as reaction additives that relieve ..
  12. Mechanisms Underlying Stress Resilience
    Janice H Urban; Fiscal Year: 2013
    ..In the BLA, NPY not only buffers the actions of CRF, but produces long term stress resilience...
  13. Novel calcium buffer for the treatment of diastolic heart failure
    Michelle L Asp; Fiscal Year: 2013
    ..Collectively, this experimental plan will enable new insights into Ca2+ mishandling in DHF and establish fundamental knowledge on using Ca2+ buffers to treat the failing heart.
  14. Secretory Pathway Calcium and Manganese Pumps
    Rajini Rao; Fiscal Year: 2013
    ..We will follow up on preliminary studies showing that a calcium handling module of pumps, channels, buffers and sensors, including SPCA2, is coordinately induced upon lactation...
  15. The role of microRNA miR-31 in skin biology
    Thomas Andl; Fiscal Year: 2013
    ..Furthermore, we will test whether miR-31 buffers certain expression patterns and signaling pathways against unwanted fluctuations and define which pathways ..
  16. Maternal behavior and effects of paternal affiliation in 5-HT1a overexpressing mi
    Juan F Lopez; Fiscal Year: 2010
    ..investigating the mechanisms whereby 5-HT1a overexpression influences maternal behavior, and how social bonding buffers the behavioral impact of this genetic alteration...
  17. Development of Biomaterials that Release Therapeutic Agents to Modulate Inflammat
    Ryan J Gilbert; Fiscal Year: 2010
    ..The hydrogel is fabricated in a chilled environment using just aqueous buffers. Thus, loaded agents are not subjected to harsh processing conditions and should be functional after release...
  18. EM STUDIES ON MUSCLE
    KENNETH ALLEN TAYLOR; Fiscal Year: 2013
    ..Rigor fibers swollen in low ionic strength buffers enhance the visibility of the 1-helical coiled-coil domain that links the myosin head to the thick filament ..
  19. Short-term plasticity &temporal precision at the inner hair cell ribbon synapse
    Elisabeth Glowatzki; Fiscal Year: 2013
    ..We will study the role of the residual intracellular Ca2+ concentration by controlling its spread with specific buffers, and by monitoring its decay time course with fluorescent dyes...
  20. The protective effect of oxytocin on social isolation: physiological mechanisms
    JASON RICHARD YEE; Fiscal Year: 2011
    ..Research has shown that OT promotes social affiliation and buffers against stressors...
  21. Electrophysiologic and Anatomic Basis of BOLD fMRI
    Nader Pouratian; Fiscal Year: 2013
    ..potential bands) that vary across cortices and tasks and are modulated by system capacities, limitations, and buffers. We hypothesize that functionally significant signals can be differentiated from non-specific activations based ..
  22. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack D Griffith; Fiscal Year: 2012
    ..allows visualization of nanogram quantities of DNA and protein and the rapid exchange DNA-protein complexes into buffers optimal for high resolution EM...
  23. Development of stable and rapidly acting adjuvanted vaccines for biodefense
    ROBERT NEWTON BREY; Fiscal Year: 2013
    ..Most vaccines are formulated in aqueous buffers and suspensions with aluminum salts adjuvants, vaccine immunogens degrade and lose potency (and efficacy)...
  24. Optimal neural and behavioral markers for learning to learn during infancy
    Rachel Wu; Fiscal Year: 2013
    ..of learning how to learn requires using bottom-up information in a systematic way, while creating top-down buffers against bottom- up distraction...
  25. Development of a Field-Deployable Device to Rapidly Measure Blood Cyanide Levels
    Hans Boehringer; Fiscal Year: 2011
    ..whole blood separation and lysis, the sample will be mixed with the on-board reagent, dried or wet, and possibly buffers. The processed sample-reagent mixture then moves through the device through various fluid paths to a detection ..
  26. Differential regulation of BMP signals in vivo.
    Kristi Wharton; Fiscal Year: 2009
    ..has a dual function of both blocking and promoting signaling; and third, a transcriptional feedback mechanism buffers the signaling gradient against stochastic or environmental fluctuations...
  27. Nucleic acid analysis using deoxyribozyme technology
    DMITRY MIKHAYLOVICH KOLPASHCHIKOV; Fiscal Year: 2010
    ..acid molecule can be detected without PCR amplification, (iv) Mild reaction conditions: the method will work in buffers close to physiological conditions and at room temperature, thus being potentially applicable in living cells, (v) ..
  28. Factors Influencing the Health Behavior of Young African American Adults
    Frederick X Gibbons; Fiscal Year: 2012
    ..willingness model as a theoretical base, we have focused on factors that moderate these influences (as buffers and risk factors), and the cognitions and dispositions that mediate them...
  29. CSF Clearance of Intracranial Debris from Traumatic Brain Injury
    Cornelius H Lam; Fiscal Year: 2013
    ..the BBB, 2) its flow is mainly egressive, and 3) it has direct access to the CSF that circulates around, rinses, buffers, and protects the brain...
  30. Zymogen activation in pancreatitis
    Fred S Gorelick; Fiscal Year: 2013
    ..preliminary data by pursuing the following specific objectives: 1) Examine the effects of standard intravenous buffers in in vivo models of mild and severe pancreatitis, 2) Investigate the effects of lactate and its receptor, GPR81, ..
  31. Parent-Child Biobehavioral Coregulation and Child Maltreatment Risk
    ERIKA LUNKENHEIMER; Fiscal Year: 2013
    ..Specific aims are to: (a) examine whether parent-child coregulation of physiology, affect, and behavior buffers families at risk for maltreatment from poor parenting, child self-regulation problems, and maltreatment incidence;..
  32. Epigenetic mechanisms of social disparities in health and development
    Daniel P Keating; Fiscal Year: 2010
    ..test the hyphothesis that variations in social status, which are putatively markers of maternal stress and stress buffers, are associated with epigenetic markers, which have been identified as being linked to the stress response system...
  33. Discrimination, Substance Use, and Cultural Buffers among AI/AN College Students
    BRENNA GREENFIELD; Fiscal Year: 2012
    ..discrimination, may contribute to these disparities, while also examining the protective effects of cultural buffers (i.e., participation in traditional activities traditional spirituality, AI/AN identity)...
  34. Multi-scale observation and modeling of IP3/Ca signaling
    John E Pearson; Fiscal Year: 2012
    ..that allow for propagation of global signals and the powerful differential modulation of this process by Ca2+ buffers of differing kinetics...
  35. Molecular and Biological Activities of Alpha Hemoglobin Stabilizing Protein
    Mitchell J Weiss; Fiscal Year: 2012
    ..Our overall view is that AHSP facilitates normal HbA synthesis and also buffers against imbalances that arise from genetic or environmental stresses, such as thalassemias and iron deficiency...
  36. Single Molecule Real Time Electronic Sequencing
    Madoo Varma; Fiscal Year: 2013
    ..Second, the presence of a high concentration of screening ions in physiological buffers greatly reduces the range and strength of electrostatic interactions...
  37. Neural Mechanisms Underlying the Protective Effects of Familism on Drug Use among
    EVA HAIMO TELZER; Fiscal Year: 2011
    ..diaries and functional magnetic resonance imaging (fMRI), to examine the neural mechanisms by which familism buffers Latino youth from drug use...
  38. Evaluate a novel product to simultaneously remove dead cells, cellular membranes,
    Christopher P Reinhardt; Fiscal Year: 2012
    ..V cannot be autoclaved, (iv) Annexin V activity can degrade over time, (v) Annexin V requires calcium-containing buffers, (vi) is difficul to use in serum, (vii) is inherently unstable due to the sloughing of Annexin V, (viii) is ..
  39. Environmental Variability, Migration, and Rural Livelihoods
    Lori M Hunter; Fiscal Year: 2011
    ..g., fuelwood, wild herbs). Natural resources also act as "buffers" against household shocks such as job loss and/or mortality...
  40. Lactic acid transport across the corneal endothelium
    TRACY THUY NGUYEN; Fiscal Year: 2013
    ..3) HCO3- together with CA II &CA XII buffers the basolateral lactate:H+ influx and HCO3- together with CA IV buffers apical lactate:H+ efflux thereby ..
  41. Implicit Association Between American And Ethnic Identities
    Thierry Devos; Fiscal Year: 2011
    ..Studies 4-6, it will be determined whether increasing the inclusion of ethnic minorities in the national identity buffers members of ethnic minorities against the adverse consequences of ethnic discrimination...
  42. Discrimination, drug use, &risky sex cognitions among young African Americans
    Michelle Stock; Fiscal Year: 2013
    ..anger and reduced self-control as potential mediators and racial- and self-affirmations as potential moderators (buffers)...
  43. EFFECTS OF BILE ACIDS ON CALCIUM AND IRON ABSORPTION
    Edward Moore; Fiscal Year: 1990
    ..The present proposal will test the hypothesis that bile salts are also important buffers for Ca++, F++ and Fe+++ ions in the small intestine...
  44. Multiplexed GPCR Characterization Using SPR
    David G Myszka; Fiscal Year: 2012
    ..the ability to print GPCRs onto a sensor surface directly from crude media using up to 96 different analysis buffers. The GPCRs were also kept wetted and active throughout the printing process by the CFM's enclosed ..
  45. Development and Validation of Novel Prospective GPS/GIS Based Exposure Measures
    Jacqueline Kerr; Fiscal Year: 2013
    ..e. using the GIS to determine time in certain locations, but not exploring route buffers or daily exposures to multiple land uses across time and space...
  46. Applicability of smartDNA Rapid Detection of Priority Agents in Food
    RICHARD BLIDNER; Fiscal Year: 2009
    ..If the food matrices have a large negative effect on the smartDNA detection assay, then the buffers and reagents will be adjusted to attempt to mitigate this effect. The detection of Y. pestis and B...
  47. Dosimetry of Lipid-Derived Amino Acid Adducts by LC/MS
    Ian Blair; Fiscal Year: 2006
    ..The use of transition metal ion-free buffers and vitamin C overcomes this problem...
  48. Genetic modification of heart performance
    JOSEPH MARK METZGER; Fiscal Year: 2010
    ..have biochemically determined variations in Mg2+ /Ca2+ binding affinities, to assess whether these novel Ca2+ buffers could correct diastolic dysfunction and preserve systolic performance. The Specific Aims are: Aim 1...
  49. Wed Based Outreach for Pregnant African American Women
    JoAnne Herman; Fiscal Year: 2001
    ..Social support buffers the effect of stressors and improves pregnancy outcomes...
  50. Continuous Chromatography Device to Economically Purify Clinical-grade Antibodies
    ANTHONY CHARLES GRABSKI; Fiscal Year: 2012
    ..process and instrument and to demonstrate up to 10-fold savings in Protein A chromatography media and buffers as compared to the traditional single-column batch method...
  51. Regulation of Amyloid Fibril Assembly by Hsp40 Molecular Chaperones
    Daniel W Summers; Fiscal Year: 2010
    ..Specific Aim 2 will address how Ydj1 interacts with the Rnq1 prion domain and buffers the accumulation of SDS-insoluble prion...
  52. Peroxidase-Based Toxicity In Hypereosinophilic States.'
    Arne Slungaard; Fiscal Year: 2006
    ..We hypothesize that SCN "buffers" against generation by EPO of the more cytotoxic N02- and Br- based oxidants and consequently serum SCN- levels, ..
  53. EPIDEMIOLOGY OF EXERTION, STRESS, AND PRETERM DELIVERY
    David Savitz; Fiscal Year: 2004
    ..We propose to evaluate the role of external stressors, perceived stress, enhancers and buffers of perceived stress, and physiologic markers of response to stress in relation to pregnancy outcome...
  54. Peer Victimization as a Pathway to Adolescent Substance Use
    Jennifer A Livingston; Fiscal Year: 2013
    ..g., gender), the characteristics of the PV experience (e.g., chronicity), and potential buffers (e.g., parent, and peer support)? 3...
  55. GENETIC DETERMINATION OF SKELETAL FRAGILITY
    Karl Jepsen; Fiscal Year: 2009
    ..interactions during growth, but the results should also provide important insight into how functional adaptation buffers the deleterious mechanical consequences of genetic variants leading to slender bone phenotypes.
  56. GENETIC DETERMINATION OF SKELETAL FRAGILITY
    Karl J Jepsen; Fiscal Year: 2013
    ..regulating trait interactions during growth, but the results should also provide important insight into how functional adaptation buffers the deleterious mechanical consequences of genetic variants leading to slender bone phenotypes.
  57. Improved Zwitterions for Proteomics
    LEO RODKEY; Fiscal Year: 2005
    ..abstract_text> ..
  58. REGULATION OF SYNAPTIC TRANSMISSION
    Robert Zucker; Fiscal Year: 2002
    ..2) How do exogenous Ca2+ buffers affect facilitation and transmission? The injection of alien fluorescent Ca2+ buffers is a useful technique for ..
  59. ACID BASE PERMEABILITY AND TRANSPORT IN COLONIC CRYPTS
    Satish Singh; Fiscal Year: 2000
    ..The colonic lumen contains high levels of ammonia, short-chain fatty acids, and CO2. These neutral buffers would be expected to readily permeate most cell membranes, and greatly affect pHi...
  60. Cystein, Intestinal Thiols and Goblet Cell Development
    H Rex Gaskins; Fiscal Year: 2007
    ..involved in both intracellular redox status and mucosal defense via its inherent contribution to the thiol buffers GSH and thioredoxin (Trx) on one hand, and the goblet cell-specific secretory mucins and intestinal trefoil ..
  61. NUTRIENT EFFECTS ON INSULIN ACTION
    Michael Pagliassotti; Fiscal Year: 2009
    ..The liver, by virtue of its anatomic position and selective regulatory features, buffers and is highly responsive to changes in nutrients...
  62. Hepatitis B virus e antigen expression
    Shuping Tong; Fiscal Year: 2004
    ..The e antigen (HBeAg) is a secreted soluble protein that promotes immune tolerance during perinatal infection and buffers immunity against HBcAg...
  63. Potentiation--noise induced hearing loss--acrylonitrile
    Laurence Fechter; Fiscal Year: 2003
    ..We hypothesize that exposure to drugs and chemical agents that disrupt intrinsic buffers of reactive oxygen species (ROS) can serve as risk factors for noise induced hearing loss by promoting ROS that ..
  64. FUNCTIONAL PROPERTIES OF CA PUMPS IN HAIR CELL
    EBENEZER YAMOAH; Fiscal Year: 2003
    ..Ca is then shuttled to the hair bundles via diffusable protein buffers. This transcellular flow of Ca maintains the active Ca extrusion from the hair bundles to the endolymph, creating ..
  65. DEPRESSION & ANXIETY DISORDER IN OLDER AFRICAN AMERICANS
    Carl Cohen; Fiscal Year: 1999
    ..variability as a means of understanding differential risk and as a means of exploring naturally-occurring buffers to mental illness...