ionophores

Summary

Summary: Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.

Top Publications

  1. ncbi Metal ionophores - an emerging class of anticancer drugs
    Wei Qun Ding
    Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    IUBMB Life 61:1013-8. 2009
  2. ncbi Polyether ionophores-promising bioactive molecules for cancer therapy
    Adam Huczyński
    Faculty of Chemistry, Department of Biochemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 780 Poznan, Poland
    Bioorg Med Chem Lett 22:7002-10. 2012
  3. ncbi Sorption and degradation in soils of veterinary ionophore antibiotics: monensin and lasalocid
    Stephen A Sassman
    Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA
    Environ Toxicol Chem 26:1614-21. 2007
  4. ncbi Ionophores: their use as ruminant growth promotants and impact on food safety
    T R Callaway
    Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, 2881 F and B Rd, Agricultural Research Service, USDA, College Station, TX 77845, USA
    Curr Issues Intest Microbiol 4:43-51. 2003
  5. ncbi PBT2 rapidly improves cognition in Alzheimer's Disease: additional phase II analyses
    Noel G Faux
    Mental Health Research Institute, The University of Melbourne, Parkville, Vic, Australia
    J Alzheimers Dis 20:509-16. 2010
  6. ncbi Effects of the ionophores valinomycin, ionomycin and gramicidin A on the element compartmentation in cultured rat hepatocytes
    S Bolkent
    University of Istanbul, Department of Biology, Istanbul, Turkey
    Toxicol In Vitro 16:159-65. 2002
  7. ncbi Free fatty acids act as endogenous ionophores, resulting in Na+ and Ca2+ influx and myocyte apoptosis
    Kwang Ming Fang
    Institute of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    Cardiovasc Res 78:533-45. 2008
  8. ncbi First divalent metal complexes of the polyether ionophore Monensin A: X-Ray structures of [Co(Mon)2(H2O)2] and [Mn(Mon)2(H2O)2] and their bactericidal properties
    Ivayla N Pantcheva
    Department of Analytical Chemistry, Faculty of Chemistry, Sofia University, 1, J Bourchier Blvd, Sofia, Bulgaria
    Curr Drug Discov Technol 5:154-61. 2008
  9. pmc Zn(2+) inhibits coronavirus and arterivirus RNA polymerase activity in vitro and zinc ionophores block the replication of these viruses in cell culture
    Aartjan J W te Velthuis
    Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Pathog 6:e1001176. 2010
  10. ncbi Copper binding agents acting as copper ionophores lead to caspase inhibition and paraptotic cell death in human cancer cells
    Saverio Tardito
    Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale e Clinica, Via Volturno 39, 43100 Parma, Italy
    J Am Chem Soc 133:6235-42. 2011

Research Grants

  1. Hypertension and Collagen: Effect of Ac-SDKP
    Nour Eddine Rhaleb; Fiscal Year: 2010
  2. ISU Vet-LIRN Collaborative Validation of Testing Methods on Animal Diagnostic Spe
    WILSON KIIZA RUMBEIHA; Fiscal Year: 2013
  3. PROGRAMMED CELL DEATH IN NORMAL AND MALIGNANT T CELLS
    Astar Winoto; Fiscal Year: 2013
  4. DERMATOREMEDIATION OF IRON OVERLOAD
    Leonard M Milstone; Fiscal Year: 2010
  5. Exploration of Chitinase Inhibitors for Human Health
    Kim D Janda; Fiscal Year: 2013
  6. Polymer Membrane Ion/Gas/Polyion Sensors: New Frontiers
    Mark E Meyerhoff; Fiscal Year: 2013
  7. ELECTRON TRANSPORT ENZYMES IN CATECHOLAMINE GRANULES
    Patrick Fleming; Fiscal Year: 1990
  8. EFFECTS OF PHORBOL ESTERS ON LYMPHOCYTE STIMULATION
    Andrea Mastro; Fiscal Year: 1991
  9. LOCATION OF PORE SYSTEMS IN CAPILLARY WALLS
    George Palade; Fiscal Year: 2000
  10. BILE ACIDS AND HEPATOCELLULAR SIGNAL TRANSDUCTION
    Bernard Bouscarel; Fiscal Year: 1999

Detail Information

Publications241 found, 100 shown here

  1. ncbi Metal ionophores - an emerging class of anticancer drugs
    Wei Qun Ding
    Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    IUBMB Life 61:1013-8. 2009
    ..We review recent work regarding the recognition of the biological effects of metal ionophores with different structures, particularly with regard to their actions upon cancer cells focusing on ..
  2. ncbi Polyether ionophores-promising bioactive molecules for cancer therapy
    Adam Huczyński
    Faculty of Chemistry, Department of Biochemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 780 Poznan, Poland
    Bioorg Med Chem Lett 22:7002-10. 2012
    ..In this article, the pharmacology and anticancer activity of the polyether ionophores undergoing pre-clinical evaluation are reviewed...
  3. ncbi Sorption and degradation in soils of veterinary ionophore antibiotics: monensin and lasalocid
    Stephen A Sassman
    Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA
    Environ Toxicol Chem 26:1614-21. 2007
    Monensin and lasalocid are polyether ionophores commonly used in the beef and poultry industries for the prevention of coccidial infections and promotion of growth...
  4. ncbi Ionophores: their use as ruminant growth promotants and impact on food safety
    T R Callaway
    Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, 2881 F and B Rd, Agricultural Research Service, USDA, College Station, TX 77845, USA
    Curr Issues Intest Microbiol 4:43-51. 2003
    b>Ionophores (such as monensin, lasalocid, laidlomycin, salinomycin and narasin) are antimicrobial compounds that are commonly fed to ruminant animals to improve feed efficiency...
  5. ncbi PBT2 rapidly improves cognition in Alzheimer's Disease: additional phase II analyses
    Noel G Faux
    Mental Health Research Institute, The University of Melbourne, Parkville, Vic, Australia
    J Alzheimers Dis 20:509-16. 2010
    ..72, p=0.056). There were no correlations between changes in CSF amyloid-beta or tau species and cognitive changes. These findings further encourage larger-scale testing of PBT2 for AD...
  6. ncbi Effects of the ionophores valinomycin, ionomycin and gramicidin A on the element compartmentation in cultured rat hepatocytes
    S Bolkent
    University of Istanbul, Department of Biology, Istanbul, Turkey
    Toxicol In Vitro 16:159-65. 2002
    ..studied by electron probe X-ray microanalysis of freeze-dried cryosections after exposure of the cells to the ionophores valinomycin, ionomycin or gramicidin A...
  7. ncbi Free fatty acids act as endogenous ionophores, resulting in Na+ and Ca2+ influx and myocyte apoptosis
    Kwang Ming Fang
    Institute of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    Cardiovasc Res 78:533-45. 2008
    ..Possible cellular mechanisms of AA/FFAs-induced myocyte apoptosis were investigated...
  8. ncbi First divalent metal complexes of the polyether ionophore Monensin A: X-Ray structures of [Co(Mon)2(H2O)2] and [Mn(Mon)2(H2O)2] and their bactericidal properties
    Ivayla N Pantcheva
    Department of Analytical Chemistry, Faculty of Chemistry, Sofia University, 1, J Bourchier Blvd, Sofia, Bulgaria
    Curr Drug Discov Technol 5:154-61. 2008
    ..The biological tests performed with the antibiotic MonH and the corresponding metal(II) complexes show greatly enhanced antimicrobial activity of complexes 2a-b against Gram(+)-bacteria...
  9. pmc Zn(2+) inhibits coronavirus and arterivirus RNA polymerase activity in vitro and zinc ionophores block the replication of these viruses in cell culture
    Aartjan J W te Velthuis
    Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Pathog 6:e1001176. 2010
    Increasing the intracellular Zn(2+) concentration with zinc-ionophores like pyrithione (PT) can efficiently impair the replication of a variety of RNA viruses, including poliovirus and influenza virus...
  10. ncbi Copper binding agents acting as copper ionophores lead to caspase inhibition and paraptotic cell death in human cancer cells
    Saverio Tardito
    Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale e Clinica, Via Volturno 39, 43100 Parma, Italy
    J Am Chem Soc 133:6235-42. 2011
    ..The establishment of a mechanism of action common to different copper binding agents provides a rationale for the exploitation of copper toxicity as an anticancer tool...
  11. ncbi Zinc ionophores pyrithione inhibits herpes simplex virus replication through interfering with proteasome function and NF-κB activation
    Min Qiu
    Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China
    Antiviral Res 100:44-53. 2013
    ..UPS inhibition by PT caused disruption of IκB-α degradation and NF-κB activation thus leading to marked reduction of viral titer. ..
  12. ncbi Determination of ionophore coccidiostats in feedingstuffs by liquid chromatography-tandem mass spectrometry Part I. Application to targeted feed
    Ursula Vincent
    European Commission, Joint Research Centre, Institute for Reference Materials and Measurements EC JRC IRMM, Retieseweg 111, B 2440 Geel, Belgium
    J Pharm Biomed Anal 47:750-7. 2008
    ..Overall, the method proposed appears to be appropriate as a confirmatory method for the monitoring of these six ionophore coccidiostats and can therefore be considered as complementary to the official HPLC-UV methods...
  13. ncbi Bacillus thuringiensis insecticidal Cry1ab toxin does not affect the membrane integrity of the mammalian intestinal epithelial cells: An in vitro study
    Nobuaki Shimada
    National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    In Vitro Cell Dev Biol Anim 42:45-9. 2006
    ....
  14. ncbi Safety, efficacy, and biomarker findings of PBT2 in targeting Abeta as a modifying therapy for Alzheimer's disease: a phase IIa, double-blind, randomised, placebo-controlled trial
    Lars Lannfelt
    Department of Public Health and Geriatrics, Uppsala University Hospital, Uppsala, Sweden
    Lancet Neurol 7:779-86. 2008
    ..Strong preclinical efficacy data and the completion of early, clinical safety studies have preceded this phase IIa study, the aim of which was to assess the effects of PBT2 on safety, efficacy, and biomarkers of AD...
  15. ncbi Effects of polyether ionophores on the protective immune responses of broiler chickens against Angara disease and Newcastle disease viruses
    K Munir
    University of Veterinary and Animal Sciences, Lahore, Pakistan
    Vet Res Commun 31:909-29. 2007
    ..NDV), an avian paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential component of a broiler medication programme in most parts of India and Pakistan...
  16. ncbi Rapid method for the simultaneous determination of six ionophores in feed by liquid chromatography/mass spectrometry
    Daljit Vudathala
    University of Pennsylvania, School of Veterinary Medicine, Department of Pathobiology, New Bolton Center Toxicology Laboratory, 382 West St Rd, Kennett Square, PA 19348, USA
    J AOAC Int 95:1016-22. 2012
    A simple and highly sensitive LC/MS method was developed for the simultaneous determination of six ionophores--lasalocid, monensin, laidlomycin, maduramycin, salinomycin, and narasin--in feed...
  17. ncbi Mechanisms of hop inhibition: hop ionophores
    Jurgen Behr
    Technische Mikrobiologie, Technische Universitat Munchen, D 85350 Freising, Germany
    J Agric Food Chem 57:6074-81. 2009
    ..The antibacterial action of iso-alpha-acids as proton ionophores could be confirmed by the BLM measurements; however, the reported ionophore properties, as electroneutral H(+)/..
  18. ncbi Oligonucleotide duplexes and multistrand assemblies with 8-aza-2'-deoxyisoguanosine: a fluorescent isoG(d) shape mimic expanding the genetic alphabet and forming ionophores
    Dawei Jiang
    Laboratory of Bioorganic Chemistry and Chemical Biology, Center for Nanotechnology, Heisenbergstrasse 11, 48149 Munster, Germany
    J Am Chem Soc 132:4016-24. 2010
    ..5'-d(T(4)4(4)T(4)) (22), containing short runs of nucleoside 4, forms stable multistranded assemblies (ionophores) with K(+) in the central cavity...
  19. pmc A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1
    Andras Balla
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:1282-95. 2005
    ..PLC activation by Ca2+ ionophores dissociated the domains from all membranes, but after Ca2+ chelation, they rapidly reassociated with the Golgi, ..
  20. ncbi Ion-selective electrodes based on L-tryptophan and L-tyrosine
    Takayo Moriuchi-Kawakami
    Department of Applied Chemistry, Faculty of Engineering, Osaka Institute of Technology, 5 16 1Omiya, Asahi, Osaka 535 8585, Japan
    Talanta 94:99-103. 2012
    ..L-Tryptophan and L-tyrosine, which are amino acids, are employed as ionophores for solvent polymeric membrane electrodes...
  21. doi Selective uranyl ion detection by polymeric ion-selective electrodes based on salphenH2 derivatives
    Dong Wan Kim
    Department of Chemistry BK21 and Research Institute of Natural Science, Gyeongsang National University, Jinju 660 701, South Korea
    Talanta 74:223-8. 2007
    ..The combination of these new ionophores with tris(2-ethylhexyl)phosphate (TEHP) as a plasticizer particularly shows near Nernstian slope in the wide ..
  22. ncbi Monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis
    Woo Hyun Park
    Division of Hematology Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
    Int J Oncol 22:855-60. 2003
    ..Taken together, these results demonstrate for the first time that monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis...
  23. ncbi Rapid and reliable identification of ionophore antibiotics in feeds by liquid chromatography-tandem mass spectrometry
    Min Huang
    Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA
    J Vet Diagn Invest 23:358-63. 2011
    ..0, which exceeds the minimum score of 4.0 required for acceptable identification of unknown compounds. The specificity of the method was achieved using retention time and multiple reaction monitoring with a total IP score of 7.0...
  24. ncbi LC/MS confirmation of ionophores in animal feeds
    S B Turnipseed
    US Food and Drug Administration, Animal Drug Research Center and Denver District Laboratory, CO 80225, USA
    J AOAC Int 84:640-7. 2001
    ..chromatographic/mass spectrometric (LC/MS) electrospray confirmation method has been developed to confirm 4 ionophores (monensin, lasalocid, salinomycin, and narasin) in a variety of animal feeds using a single quadrupole mass ..
  25. ncbi Rapid forensic selected reaction monitoring liquid chromatography/mass spectrometry determination of ionophore antibiotics found at toxic levels in animal feeds
    Joseph G Ebel
    Cornell University, Analytical Toxicology, College of Veterinary Medicine, 927 Warren Dr, Ithaca, NY 14850, USA
    J AOAC Int 87:25-30. 2004
    ..Control feed samples fortified with monensin at concentrations from 50 ppb to 5 ppm provided a linear response and calibration curve across this range with a correlation coefficient of 0.996...
  26. ncbi Determination of the ionophoric coccidiostats narasin, monensin, lasalocid and salinomycin in eggs by liquid chromatography/tandem mass spectrometry
    Leen Mortier
    Ministry of the Flemish Community, Agricultural Research Centre Ghent CLO, Department of Animal Product Quality and Transformation Technology DVK, Brusselsesteenweg 370, 9090 Melle, Belgium
    Rapid Commun Mass Spectrom 19:533-9. 2005
    ..1 CC(alpha) was found to be 1 microg/kg for all four compounds. Monitoring of Belgian egg samples in 2004 revealed that residues of salinomycin, lasalocid and monensin could be found...
  27. ncbi Cyclosporin A does not protect the disruption of the inner mitochondrial membrane potential induced by potassium ionophores in intact K562 cells
    Luis F Marques-Santos
    Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraiba, Campus I, Cidade Universitaria, CEP 58059 900, Joao Pessoa, PB, Brazil
    Cell Biol Int 30:197-204. 2006
    ....
  28. ncbi Store-mediated calcium entry in the regulation of phosphatidylserine exposure in blood cells from Scott patients
    Imke C A Munnix
    Department of Biochemistry, CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 89:687-95. 2003
    ..patient V.W. is a new case of Scott syndrome, ii. Ca(2+) entry in the platelets and lymphoblasts from both Scott patients is normal, and iii. elevated [Ca(2+)](i) as caused by SMCE is not sufficient to trigger PS exposure...
  29. ncbi Determination of lasalocid sodium in animal feeds and premixes by reversed-phase liquid chromatography: collaborative study
    Charles Focht
    Nebraska Department of Agriculture, 3703 S 14th St, Lincoln, NE 68502, USA
    J AOAC Int 91:479-88. 2008
    ..RSDr values for the 2 supplemental trace-level samples ranged from 1.6 to 2.5%, RSDR values ranged from 5.6 to 9.2%, and HorRat values ranged from 0.43 to 0.62...
  30. ncbi Lasalocid toxicosis is inadequately quantified for horses
    David S Kronfeld
    Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061 0506, USA
    Vet Hum Toxicol 44:245-7. 2002
    ..In contrast, the whole data set suggests a lowest toxic dose of 15 mg/kg, although this estimate was based on only 1 affected animal in 8 tests from 5 to 15 mg/kg in an unbalanced experiment and thus needs confirmation...
  31. pmc Effect of monensin on survival and growth of Escherichia coli O 157:H7 in vitro
    Susan J Bach
    Agriculture and Agri Food Canada, Lethbridge Research Centre, Lethbridge, Alberta
    Can Vet J 43:718-9. 2002
  32. ncbi Acute and long-term cardiomyopathy and delayed neurotoxicity after accidental lasalocid poisoning in horses
    A Decloedt
    Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Belgium
    J Vet Intern Med 26:1005-11. 2012
    ..Horses are extremely susceptible to ionophore intoxication. Although numerous reports are available regarding monensin, little is known about lasalocid toxicity...
  33. ncbi Effect of phloretin on ionophore mediated electroneutral transmembrane translocations of H(+), K(+) and Na(+) in phospholipid vesicles
    S Bala
    Department of Electrical Engineering, Indian Institute of Technology, Bombay, Mumbai, India
    Biochim Biophys Acta 1510:258-69. 2001
    ..The transporters used in this study are (a) the exchange ionophores: nigericin, monensin; (b) combinations of alkali metal ion carriers, valinomycin or nonactin with weak acids ..
  34. ncbi Synergistic fungicidal activities of polymyxin B and ionophores, and their dependence on direct disruptive action of polymyxin B on fungal vacuole
    Akira Ogita
    Research Center for Urban Health and Sports, Osaka City University, Sumiyoshi ku, Osaka, Japan
    J Antibiot (Tokyo) 62:81-7. 2009
    ..PMB was shown to exhibit marked fungicidal activity against yeasts and filamentous fungi in combination with ionophores such as salinomycin (SAM) and monensin (MON), which can selectively interact with monovalent cations...
  35. ncbi Exocytotic release of ATP from cultured astrocytes
    Tina Pangrsic
    Laboratory of Neuroendocrinology Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Zaloska 4, SI 1000 Ljubljana, Slovenia
    J Biol Chem 282:28749-58. 2007
    ....
  36. pmc Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells
    Jyoti K Jaiswal
    Laboratory of Cellular Biophysics, Rockefeller University, New York, NY 10021, USA
    J Cell Biol 159:625-35. 2002
    ..The lysosomes that fused were predominantly predocked at the plasma membrane, indicating that calcium is primarily responsible for fusion and not recruitment of lysosomes to the cell surface...
  37. ncbi Environmental occurrence and shallow ground water detection of the antibiotic monensin from dairy farms
    Naoko Watanabe
    Dep of Land, Air and Water Resources, Veihmeyer Hall, Univ of California, Davis, CA 95616 8628, USA
    J Environ Qual 37:S78-85. 2008
    ..The data suggest the possibility of monensin transport into shallow (2-5 m) alluvial ground water from dairy management units, including manure storage lagoons and freestalls occupied by heifers, lactating cows, and dry cows...
  38. pmc Reversible Ca2+-induced fast-to-slow transition in primary skeletal muscle culture cells at the mRNA level
    J D Meissner
    Zentrum Physiologie, Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    J Physiol 523:19-28. 2000
    ....
  39. pmc Calcineurin regulates slow myosin, but not fast myosin or metabolic enzymes, during fast-to-slow transformation in rabbit skeletal muscle cell culture
    J D Meissner
    Zentrum Physiologie, Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    J Physiol 533:215-26. 2001
    ..The data indicate a differential regulation of MHCI, of MHCII and of metabolism. Calcineurin alone is not sufficient to mediate the complete transformation...
  40. ncbi Activation of the beta myosin heavy chain promoter by MEF-2D, MyoD, p300, and the calcineurin/NFATc1 pathway
    Joachim D Meissner
    Department of Physiology, Hannover Medical School, Hannover, Germany
    J Cell Physiol 211:138-48. 2007
    ..Together, our findings demonstrate calcium-ionophore-induced activation of the beta MyHC promoter by NFATc1, MyoD, MEF-2D, and p300 in a calcineurin-dependent manner...
  41. ncbi Cyclodextrin-based potentiometric sensors for midazolam and diazepam
    C G Amorim
    REQUIMTE, Servico de Quimica Fisica, Fac Farmácia U P, Rua Anibal Cunha 164, 4099 030 Porto, Portugal
    J Pharm Biomed Anal 48:1064-9. 2008
    ..on polymeric membranes incorporating respectively beta-cyclodextrin and (2-hydroxiproyl)-gamma-cyclodextrin as ionophores, 2-fluorophenyl 2-nitrophenyl ether as plasticizer and potassium tetrakis (p-chlorophenyl) borate as ionic ..
  42. ncbi Study of resistance against some ionophores in Eimeria tenella field isolates
    A Yadav
    Department of Veterinary Parasitology, CCS Haryana Agricultural University, Hisar-125 004, India
    Vet Parasitol 102:69-75. 2001
    ..Haryana (north India) were studied in a battery test for evaluating drug resistance against two currently used ionophores; maduramicin (5ppm) and salinomycin (60ppm)...
  43. ncbi Phosphorated calix[6]arene derivatives as an ionophore for atropine-selective membrane electrodes
    Mohsen M Zareh
    Warsaw Technical University, Department of Analytical Chemistry, Warsaw 00664, Poland
    J AOAC Int 90:147-52. 2007
    ..41,42-pentol were introduced as neutral ionophores for atropine-selective electrodes. Practical Nernstian responses were found (54.3, 49.1, and 50...
  44. ncbi New ionophores for vitamin B1 and vitamin B6 potentiometric sensors for multivitaminic control
    Ana Rita Pires
    REQUIMTE, Dep de Química Física, Faculdade de Farmacia, UP Rua Aníbal Cunha 164, 4099 030 Porto, Portugal
    J Pharm Biomed Anal 46:683-91. 2008
    ..The solid contact electrodes were produced using beta-cyclodextrins as ionophores in a carboxylated poly(vinyl chloride) support matrix. Near Nernstian slopes (mV/decade) of 51.7+/-0.8, 60.6+/-0...
  45. ncbi Schiff bases as cadmium(II) selective ionophores in polymeric membrane electrodes
    V K Gupta
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Anal Chim Acta 583:340-8. 2007
    The construction and performance characteristics of polymeric membrane electrodes based on two neutral ionophores, N,N'-[bis(pyridin-2-yl)formylidene]butane-1,4-diamine (S1) and N-(2-pyridinylmethylene)-1,2-benzenediamine (S2) for ..
  46. ncbi A fluorescent sensor with high selectivity and sensitivity for potassium in water
    Huarui He
    Roche Diagnostic Corporation, 235 Hembree Park Drive, Roswell, Georgia 30076, USA
    J Am Chem Soc 125:1468-9. 2003
    ..Excitation and emission occur in the visible light region. This new potassium sensor is currently used in the Roche OPTI CCA, a commercially available whole blood analyzer...
  47. ncbi No role for Ca++ or protein kinase C in alpha-1A adrenergic receptor activation of mitogen-activated protein kinase pathways in transfected PC12 cells
    A Berts
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia, USA
    Mol Pharmacol 55:296-303. 1999
    ..The results suggest that Ca++ release and PKC activation are neither necessary nor sufficient for alpha-1A AR-mediated activation of mitogenic responses in PC12 cells...
  48. ncbi Intracellular calcium and myosin isoform transitions. Calcineurin and calcium-calmodulin kinase pathways regulate preferential activation of the IIa myosin heavy chain promoter
    David L Allen
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    J Biol Chem 277:45323-30. 2002
    ..Downstream mediators of these effects are the activities of the MEF-2 and NFAT transcription factors, whose binding sites in the MyHC IIa promoter are required for calcium-induced activation of the MyHC IIa promoter...
  49. ncbi Benchmarking of linear and nonlinear approaches for quantitative structure-property relationship studies of metal complexation with ionophores
    Igor V Tetko
    Institute of Bioorganic and Petrochemistry, Kiev, Ukraine
    J Chem Inf Model 46:808-19. 2006
    ..The current study illustrates the difficulties of quantitative comparison of different methods: conclusions based only on one data set without appropriate statistical tests could be wrong...
  50. ncbi Pharmacological actions of monovalent ionophores on spontaneously beating rabbit sino-atrial nodal cells
    H Satoh
    Department of Pharmacology, Nara Medical University, Kashihara, Japan
    Gen Pharmacol 33:151-9. 1999
    The effects of sodium (monensin) and potassium (nigericin and lonomycin A) ionophores on the spontaneous activity in rabbit sino-atrial (SA) nodal cells were investigated using microelectrode and whole-cell voltage-clamp techniques...
  51. ncbi Chromium(III) selective membrane sensors based on Schiff bases as chelating ionophores
    A K Singh
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Anal Chim Acta 585:171-8. 2007
    ..and N,N'-bis(acetoacetanilide)-triethylenetetraammine (L(2)), have been synthesized and explored as neutral ionophores for preparing poly(vinylchloride) (PVC) based membrane sensors selective to Cr(III)...
  52. ncbi Fiber type conversion alters inactivation of voltage-dependent sodium currents in murine C2C12 skeletal muscle cells
    Eva Zebedin
    Institut fur Pharmakologie, Medizinische Universität Wien, Währinger Strasse 13a, A 1090 Vienna, Austria
    Am J Physiol Cell Physiol 287:C270-80. 2004
    ..5 compared with the skeletal muscle isoform Na(v)1.4. These results imply that fast-to-slow fiber type conversion of skeletal muscle cells involves functional adaptation of their electrophysiological properties...
  53. ncbi Ca2+ transients activate calcineurin/NFATc1 and initiate fast-to-slow transformation in a primary skeletal muscle culture
    Hans Peter Kubis
    Zentrum Physiologie, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    Am J Physiol Cell Physiol 285:C56-63. 2003
    ....
  54. ncbi The p38alpha/beta mitogen-activated protein kinases mediate recruitment of CREB-binding protein to preserve fast myosin heavy chain IId/x gene activity in myotubes
    Joachim D Meissner
    Department of Physiology, Hannover Medical School, D 30625 Hannover, Germany
    J Biol Chem 282:7265-75. 2007
    ..Taken together, the data indicate that p38alpha/beta MAPKs-mediated coactivator recruitment at a proximal MEF-2 site is important for MyHCIId/x gene regulation in skeletal muscle...
  55. ncbi Intracellular mechanisms of hypoxia-induced calcium increase in rat sensory neurons
    E A Lukyanetz
    Bogomoletz Institute of Physiology, 01024 Kiev, Ukraine
    Arch Biochem Biophys 410:212-21. 2003
    ..Mitochondria are important for spatial difference in the hypoxia-induced Ca(2+) elevation due to their specific location in these neurons...
  56. ncbi Calmodulin-dependent and -independent apoptosis in cell of a Drosophila neuronal cell line
    K Ui-Tei
    Department of Pharmacology, Nippon Medical School, Bunkyo ku, Tokyo 113 8602, Japan
    Apoptosis 5:133-40. 2000
    ..One pathway induced by H-7, actinomycin D or cycloheximide is calmodulin-dependent (pathway H), and another induced by A23187 is calmodulin-independent (pathway A)...
  57. ncbi Mitochondria are intracellular magnesium stores: investigation by simultaneous fluorescent imagings in PC12 cells
    Takeshi Kubota
    School of Fundamental Science and Technology, Keio University, 3 14 1 Hiyoshi, Yokohama, Kanagawa 223 8522, Japan
    Biochim Biophys Acta 1744:19-28. 2005
    ..These findings show that the source of Mg2+ in the FCCP-induced [Mg2+]i increase in PC12 cells is mitochondria, and that mitochondrial depolarization triggers the Mg2+ release...
  58. pmc Synthesis and anticancer properties of water-soluble zinc ionophores
    Darren Magda
    Pharmacyclics, Inc, Sunnyvale, California 94085, USA
    Cancer Res 68:5318-25. 2008
    ..Gene expression profiles of A549 cultures treated with one of these water-soluble zinc ionophores, PCI-5002, reveal the activation of stress response pathways under the control of metal-responsive transcription ..
  59. pmc HIV-1 fusion peptide targets the TCR and inhibits antigen-specific T cell activation
    Francisco J Quintana
    Department of Immunology, Weizmann Institute of Science, Rehovot, Israel
    J Clin Invest 115:2149-58. 2005
    ..Disassociated from HIV, however, the FP molecule provides a novel reagent for downregulating undesirable immune responses, exemplified here by adjuvant arthritis...
  60. ncbi Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases
    R Alberio
    Department of Molecular Animal Breeding and Genetics, University of Munich, Oberschleissheim, Germany
    Mol Reprod Dev 55:422-32. 2000
    ..This effect can be improved by combination with ionomycin. The targeting of CDKs in the way to activate bovine oocytes can be an approach to improve the efficiency of mammalian oocyte activation...
  61. ncbi Efficacy of ionophores in cattle diets for mitigation of enteric methane
    H Guan
    Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
    J Anim Sci 84:1896-906. 2006
    Use of ionophores in cattle diets has been proposed as a strategy for mitigation of enteric CH4 emissions...
  62. ncbi Expression of cytosolic phospholipase A2 alpha in murine C12 cells, a variant of L929 cells, induces arachidonic acid release in response to phorbol myristate acetate and Ca2+ ionophores, but not to tumor necrosis factor-alpha
    Masaya Shimizu
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan
    J Pharmacol Sci 96:324-32. 2004
    ..The role of Ser505 phosphorylation in AA release induced by PMA is also discussed...
  63. ncbi On the relationship between matrix free Mg2+ concentration and total Mg2+ in heart mitochondria
    D W Jung
    Department of Medical Biochemistry, Ohio State University, Columbus 43210 1218, USA
    Biochim Biophys Acta 1320:310-20. 1997
    ..This suggests that [Mg2+]m is well-buffered during osmotic volume changes unless there is also a change in ligand availability...
  64. ncbi A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components
    Frida Henningsson
    Swedish University of Agricultural Sciences, Department of Molecular Biosciences, The Biomedical Center, 751 23 Uppsala, Sweden
    FEBS J 273:4901-12. 2006
    ....
  65. pmc Identification and characterization of a newly recognized population of high-Na+, low-K+, low-density sickle and normal red cells
    R M Bookchin
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 97:8045-50. 2000
    ..This paper focuses on the discovery, basic characterization, and exclusion of artifactual origin of this RBC fraction. Many future studies will be needed to clarify their mechanism of generation and full pathophysiological significance...
  66. ncbi A fast and simple screening test to search for specific inhibitors of the plasma membrane calcium pump
    T Tiffert
    Physiological Laboratory, University of Cambridge, United Kingdom
    J Lab Clin Med 137:199-207. 2001
    ..Vanadate was used as a test substance to assess the effect of putative pump inhibitors. This method offers a cost-efficient and easily automated alternative for testing large numbers of natural or synthetic agents...
  67. ncbi Distribution of dehydration rates generated by maximal Gardos-channel activation in normal and sickle red blood cells
    Virgilio L Lew
    Department of Physiology, University of Cambridge, Cambridge, United Kingdom
    Blood 105:361-7. 2005
    ..The results support the view that stochastic Ca2+ permeabilization rather than Gardos-channel variation is the main determinant selecting which SS cells dehydrate through Gardos channels in each sickling episode...
  68. pmc The chymase, mouse mast cell protease 4, constitutes the major chymotrypsin-like activity in peritoneum and ear tissue. A role for mouse mast cell protease 4 in thrombin regulation and fibronectin turnover
    Elena Tchougounova
    Swedish University of Agricultural Sciences, Department of Molecular Biosciences, The Biomedical Center, Box 575, 751 23 Uppsala, Sweden
    J Exp Med 198:423-31. 2003
    ..Further, a role for mMCP-4 in connective tissue remodeling was suggested by the inability of mMCP-4 null peritoneal cells to process endogenous fibronectin...
  69. ncbi Ca2+ ionophores trigger membrane remodeling without a need for store-operated Ca2+ entry
    Marie Galitzine
    INSERM Unité 143, 80 rue du General Leclerc, 94276, Le Kremlin Bicetre, France
    Biochem Biophys Res Commun 327:335-41. 2005
    Calcium (Ca2+) ionophores are the most effective agents able to elicit rapid membrane remodeling in vitro...
  70. pmc Deciphering the plasma membrane hallmarks of apoptotic cells: phosphatidylserine transverse redistribution and calcium entry
    M C Martinez
    Institut d Hématologie et d Immunologie, Faculte de Medecine, Universite Louis Pasteur, 4, rue Kirschleger, 67085, Strasbourg, France
    BMC Cell Biol 2:20. 2001
    ..We have recently reported a reduced capacitative Ca2+ entry in Scott cells which may be part of the Scott phenotype...
  71. pmc Delayed activation of the store-operated calcium current induced by calreticulin overexpression in RBL-1 cells
    C Fasolato
    Department of Biomedical Sciences, National Research Center for Biomembranes, University of Padova, I 35121 Padova, Italy
    Mol Biol Cell 9:1513-22. 1998
    ..The present results are compatible with a major Ca2+-buffering role of CRT within the ER but exclude a direct, or indirect, role of this protein on the mechanism of ICRAC activation...
  72. pmc Transport properties of the calcium ionophore ETH-129
    E Wang
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biophys J 81:3275-84. 2001
    ..These properties suggest that ETH-129 will be useful for investigating the consequences of a mitochondrial Ca(2+) overload in mammalian cells, which is difficult to pursue through the application of electroneutral ionophores.
  73. ncbi Mitochondrial permeability transition in neuronal damage promoted by Ca2+ and respiratory chain complex II inhibition
    Evelise N Maciel
    Departamento de Patologia Clinica, Faculdade de Ciencias Medicas, Universidade Estadual de Campinas, Campinas, Brazil
    J Neurochem 90:1025-35. 2004
    ..We propose that under conditions in which mitochondrial complex II is partially inhibited in the CNS, neuronal cell death involves the induction of PT...
  74. pmc Immediate and delayed VEGF-mediated NO synthesis in endothelial cells: role of PI3K, PKC and PLC pathways
    David S Gelinas
    Department of Pharmacology, University of Montreal, Montreal QC, Canada
    Br J Pharmacol 137:1021-30. 2002
    ..5. In conclusion, VEGF induces an immediate cGMP synthesis through the PLC-Ca2+/CaM pathway, and that the induction of delayed cGMP synthesis implies Akt and PKC activity...
  75. ncbi Calcium-dependent expression of TNF-alpha in neural cells is mediated by the calcineurin/NFAT pathway
    Andrea Canellada
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas CBM CSIC, Universidad Autonoma de Madrid UAM, Facultad de Ciencias, Madrid 28049, Spain
    Mol Cell Neurosci 31:692-701. 2006
    ..Given proposed functions of TNF-alpha in spatial learning, memory and the pathogenesis of neurodegenerative diseases, the data presented suggest an important role for calcineurin/NFAT signaling in these key neurological processes...
  76. ncbi Calcium influx pathways in rat CNS pericytes
    Masahiro Kamouchi
    Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi, Fukuoka 812 8582, Japan
    Brain Res Mol Brain Res 126:114-20. 2004
    ....
  77. ncbi Regulation of growth hormone release in common carp pituitary cells by pituitary adenylate cyclase-activating polypeptide: signal transduction involves cAMP- and calcium-dependent mechanisms
    Dong Xiao
    Department of Zoology, University of Hong Kong, China
    Neuroendocrinology 76:325-38. 2002
    ..Apparently, the GH-releasing action of PACAP is mediated through the adenylate cyclase/cAMP/protein kinase A pathway and [Ca(2+)](e) influx through VSCC...
  78. ncbi Translocation of phospholipase A2 to membranes by oxidized LDL and hydroxyoctadecadienoic acid to contribute to cholesteryl ester formation
    Satoshi Akiba
    Department of Pathological Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Biochim Biophys Acta 1686:77-84. 2004
    ....
  79. ncbi Actions of ionomycin, 4-BrA23187 and a novel electrogenic Ca2+ ionophore on mitochondria in intact cells
    Andrey Y Abramov
    Department of Physiology and Mitochondrial Biology Group, University College London, Gower Street, London WC1E 6BT, UK
    Cell Calcium 33:101-12. 2003
    We have used fluorescence digital imaging techniques to explore the actions of two groups of Ca(2+) ionophores: (i). ferutinin, an electrogenic naturally occurring ionophore, and (ii)...
  80. ncbi Glia re-sealed particles freshly prepared from adult rat brain are competent for exocytotic release of glutamate
    Sara Stigliani
    Pharmacology and Toxicology Section, Department of Experimental Medicine, University of Genoa, Genoa, Italy
    J Neurochem 96:656-68. 2006
    ..It is concluded that gliosomes purified from adult brain contain glutamate-accumulating vesicles and can release the amino acid by a process resembling neuronal exocytosis...
  81. ncbi External Ca2+ dependency of synaptic transmission in drosophila synaptotagmin I mutants
    Tomonori Okamoto
    Gunma University Graduate School of Medicine, Maebashi, Japan
    J Neurophysiol 94:1574-86. 2005
    ..These findings led us to conclude that C2B, not C2A, senses Ca2+, and Syt I is a negative regulator of vesicle fusion...
  82. ncbi Excitotoxic injury to mitochondria isolated from cultured neurons
    Yulia E Kushnareva
    MitoKor, San Diego, CA 92121, USA
    J Biol Chem 280:28894-902. 2005
    ....
  83. ncbi Cremophor EL, a non-ionic surfactant, promotes Ca(2+)-dependent process of cell death in rat thymocytes
    Jun Ya Yamaguchi
    Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770 8502, Japan
    Toxicology 211:179-86. 2005
    ..It is suggested that cremophor EL at clinically-relevant concentrations augments the Ca(2+)-dependent process of necrosis...
  84. ncbi Activation of GPCRs modulates quantal size in chromaffin cells through G(betagamma) and PKC
    Xiao Ke Chen
    Institute of Neuroscience, Shanghai Institutes for Biological Sciences and Graduate School, Chinese Academy of Sciences, Shanghai 200031, China
    Nat Neurosci 8:1160-8. 2005
    ..Thus, a variety of G protein pathways exist to modulate Ca2+-evoked secretion at specific steps in fusion pore formation...
  85. ncbi [Effect of alpha-tocopherol on apoptosis and necrosis of rat thymocytes induced by calcium ionophore A23187 in various concentrations]
    G V Petrova
    Ukr Biokhim Zh 76:103-7. 2004
    ..It was proposed that such effect can be explained by the ability of alpha-tocopherol to prevent the mitochondrial permeability transition--a key event in apoptosis and necrosis induction...
  86. ncbi Down-regulation of CYP1A2 induction during the maturation of mouse cerebellar granule cells in culture: role of nitric oxide accumulation
    Sonia Mulero-Navarro
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Eur J Neurosci 18:2265-72. 2003
    ..Increased NMDA receptor activity and Ca2+ entry could contribute to this process. Thus, neurodegeneration could diminish the induction of specific P450s and impair the metabolism of foreign and/or endogenous chemicals in the CNS...
  87. ncbi Expression and modulation of an NADPH oxidase in mammalian astrocytes
    Andrey Y Abramov
    Department of Physiology, University College London, London WC1E 6BT, United Kingdom
    J Neurosci 25:9176-84. 2005
    ..The astrocytic NADPH oxidase is likely to play important roles in CNS physiology and pathology...
  88. ncbi Lysophosphatidic acid induces histamine release from mast cells and skin fragments
    Terumasa Hashimoto
    Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Pharmacology 75:13-20. 2005
    ..These findings suggest that LPA-induced histamine release may be attributable to calcium release from intracellular stores. Moreover, ROCK participates in the extracellular calcium-independent process of LPA-induced histamine release...
  89. ncbi Ionic mechanisms of excitation-induced regulation of Na+-K+-ATPase mRNA expression in isolated rat EDL muscle
    K T Murphy
    School of Human Movement, Recreation and Performance, Centre for Ageing, Rehabilitation and Sport Science, Victoria University of Technology, Melbourne, Australia
    Am J Physiol Regul Integr Comp Physiol 290:R1397-406. 2006
    ..Furthermore, Na+-K+-ATPase mRNA appears to be regulated by different stimuli, including cellular changes associated with membrane depolarization and increased intracellular Ca2+ content but not increased intracellular Na+ content...
  90. ncbi Monitoring cytosolic calcium in the dinoflagellate Crypthecodinium cohnii with calcium orange-AM
    Connie M C Lam
    Biology Department, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, PR China
    Plant Cell Physiol 46:1021-7. 2005
    ..The dye fluorescence responded well to the effects of calcium ionophores and calcium chelators...
  91. ncbi Calcium ionophore A23187 action on cardiac myocytes is accompanied by enhanced production of reactive oxygen species
    Tomasz Przygodzki
    Department of General Biophysics, University of Lodz, Poland
    Biochim Biophys Acta 1740:481-8. 2005
    ..Their effect appeared to be independent of phospholipase A(2) inhibition, suggesting that COX and LOX stimulation is not due to elevated substrate (arachidonic acid) concentration but rather to a direct effect of calcium...
  92. ncbi Chlorpromazine inhibits the glucocorticoid receptor-mediated gene transcription in a calcium-dependent manner
    A Basta-Kaim
    Department of Endocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna Street, 31 343 Krakow, Poland
    Neuropharmacology 43:1035-43. 2002
    ..The inhibitory effect of chlorpromazine on the corticosterone-induced gene transcription seems to depend on the inhibition of Ca(2+) influx and/or the inhibition of some calcium-dependent enzymes, e.g. phospholipase beta(1)...
  93. ncbi Cleavage of the cyclin-dependent kinase 5 activator p35 to p25 does not induce tau hyperphosphorylation
    Petri Kerokoski
    Department of Neuroscience and Neurology, University of Kuopio, Finland
    Biochem Biophys Res Commun 298:693-8. 2002
    ..Treatment of the neuronal cultures with N-methyl-D-aspartic acid or with calcium ionophores resulted in an outcome highly similar to that of glutamate...
  94. ncbi Calcium signaling in excystation of the early diverging eukaryote, Giardia lamblia
    David S Reiner
    Department of Pathology, University of California, San Diego, 92103, USA
    J Biol Chem 278:2533-40. 2003
    ..acid-tetra(acetyloxymethyl) ester, a Ca(2+) channel blocker, Ca(2+) ionophores, and thapsigargin) strongly inhibit excystation...
  95. ncbi Phorbol ester up-regulates capacities for nuclear translocation and phosphorylation of 5-lipoxygenase in Mono Mac 6 cells and human polymorphonuclear leukocytes
    O Werz
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, Stockholm, Sweden
    Blood 97:2487-95. 2001
    ....
  96. ncbi NID67, a small putative membrane protein, is preferentially induced by NGF in PC12 pheochromocytoma cells
    L Vician
    Department of Biological Chemistry, UCLA Center for the Health Sciences, Los Angeles, California, USA
    J Neurosci Res 64:108-20. 2001
    ..Several small membrane proteins are similar to NID67; they contain a transmembrane domain and little more. All of these proteins participate in forming or regulating ion channels. NID67 may play a similar role in cellular physiology...
  97. ncbi Functional receptor-channel coupling compared in contractile and proliferative human vascular smooth muscle
    T Karkanis
    Department of Physiology, The University of Western Ontario, London, Ontario, Canada
    J Cell Physiol 187:244-55. 2001
    ..These studies demonstrate the usefulness of this human vascular cell clone for studying functional plasticity of smooth muscle, while avoiding complications arising from extended times in culture...
  98. ncbi Novel neuroprotective compound SCH-20148 rescues thymocytes and SH-SY5Y cells from thapsigargin-induced mitochondrial membrane potential reduction and cell death
    Yuko Muramatsu
    Pharmacology Research Laboratories, Astellas Pharma Inc, 5 2 3, Tokodai, Tsukuba, Ibaraki, 300 2698, Japan
    Eur J Pharmacol 563:40-8. 2007
    ..In addition, SH-20148 demonstrated a neuroprotective effect against thapsigargin-induced neuronal cell death in neuroblastoma SH-SY5Y cells. Taken together, these results suggest the potential of SCH-20148 as novel neuroprotective drug...
  99. ncbi Ca2+-RhoA signaling pathway required for polyamine-dependent intestinal epithelial cell migration
    J N Rao
    Department of Surgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 280:C993-1007. 2001
    ..These findings suggest that polyamine-dependent cell migration is partially initiated by the formation of myosin II stress fibers as a result of Ca2+-activated RhoA activity...
  100. ncbi Regulation of SERCA Ca2+ pump expression by cytoplasmic Ca2+ in vascular smooth muscle cells
    K D Wu
    Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan 100, Republic of China
    Am J Physiol Cell Physiol 280:C843-51. 2001
    ..We conclude that changes in cytosolic Ca2+ control the expression of SERCA2b in VSMC via a mechanism involving a currently uncharacterized, H-7-sensitive but staurosporine-insensitive, protein kinase...
  101. ncbi Adenosine signaling in outer medullary descending vasa recta
    E P Silldorff
    Biology Department, Towson University, Towson, Baltimore, Maryland 21201, USA
    Am J Physiol Regul Integr Comp Physiol 280:R854-61. 2001
    ..We conclude that OMDVR vasodilation by adenosine involves cAMP and COX but not NO. COX blockade does not fully inhibit selective A(2) receptor-mediated OMDVR dilation...

Research Grants62

  1. Hypertension and Collagen: Effect of Ac-SDKP
    Nour Eddine Rhaleb; Fiscal Year: 2010
    ..on Ang II and ET-1-stimulated transactivation of the EGFR;2) whether Ac-SDKP inhibits the effects of calcium ionophores or EGF on p42/44 MAPK and collagen synthesis;3) whether PLC, EGFR, cSrc, calmodulin kinase or IP3 inhibitors ..
  2. ISU Vet-LIRN Collaborative Validation of Testing Methods on Animal Diagnostic Spe
    WILSON KIIZA RUMBEIHA; Fiscal Year: 2013
    ..labs to help them validate tests for which our lab has a unique expertise in including aflatoxins, Ractopamine, ionophores, ergot alkaloids, bromethalin, Cholecalciferol, selenium, coumesterol, zearalenone and cyanotoxins...
  3. PROGRAMMED CELL DEATH IN NORMAL AND MALIGNANT T CELLS
    Astar Winoto; Fiscal Year: 2013
    ..In some cancer cell lines, however, apoptotic-inducing agents like calcium ionophores and phorbol ester were shown to initiate apoptosis through translocation of Nur77 to mitochondria...
  4. DERMATOREMEDIATION OF IRON OVERLOAD
    Leonard M Milstone; Fiscal Year: 2010
    ..by topical application of iron ionophores or iron chelators; B...
  5. Exploration of Chitinase Inhibitors for Human Health
    Kim D Janda; Fiscal Year: 2013
    ..upon closantel's general scaffolding, we have explored both small molecule OvCHT1 inhibitors and proton ionophores as anti-molting agents, as closantel possesses both of these biochemical activities...
  6. Polymer Membrane Ion/Gas/Polyion Sensors: New Frontiers
    Mark E Meyerhoff; Fiscal Year: 2013
    ..can be employed for similar measurements;2) further optimize the utility of rhodium(III) metalloporphyrins as ionophores in polymeric films for developing highly selective electrochemical and optical sensors for nitrite (using capped ..
  7. ELECTRON TRANSPORT ENZYMES IN CATECHOLAMINE GRANULES
    Patrick Fleming; Fiscal Year: 1990
    ..For this objective we will use phospholipid vesicle reconstitution, ionophores to create gradients and spectrophotometric assay of the cytochrome function...
  8. EFFECTS OF PHORBOL ESTERS ON LYMPHOCYTE STIMULATION
    Andrea Mastro; Fiscal Year: 1991
    ..Wheat germ agglutinnin (WGA) a non-mitogenic lectin is rendered mitogenic by TPA. TPA and calcium ionophores, by-pass the need for lectins. Finally TPA and IL2 are mitogenic...
  9. LOCATION OF PORE SYSTEMS IN CAPILLARY WALLS
    George Palade; Fiscal Year: 2000
    ..negative and possible positive modulators of transcytosis, and they include NEM, Na-nitroprusside, ANP and Ca++ ionophores. In these experiments, alterations, if any, in the interendothelial junctions will be investigated. IV...
  10. BILE ACIDS AND HEPATOCELLULAR SIGNAL TRANSDUCTION
    Bernard Bouscarel; Fiscal Year: 1999
    ..of bile acid uptake in isolated hepatocytes: 1) the ability of calcium-mobilizing hormones and calcium ionophores to alter hepatocellular bile acid uptake will be confirmed; 2) the respective effect of intracellular and ..
  11. MOLECULAR BASIS OF NERVE GROWTH FACTOR
    Jeffrey Milbrandt; Fiscal Year: 1993
    ..genes, c-fos, NGFI-A and NGFI-B, are rapidly transcribed in response to NGF, phorbol esters (TPA), and calcium ionophores (A23187), and the induction process does not require de novo protein synthesis...
  12. IONOPHORES, TRANSPORT AND MEMBRANE STRUCTURE
    William Duax; Fiscal Year: 1980
    ..calculations (1) solid state data concerning complexed and uncomplexed forms of valinomycin, monensin, and other ionophores will be used to model the dynamics of ion capture and release processes, (2) data on angiotensin and alamethicin ..
  13. NMR STUDY OF METAL-ION COMPLEXATION
    ANTHONY FRATIELLO; Fiscal Year: 1990
    ..of nuclear magnetic resonance (NMR) spectroscopy to the study of metal-ion complexes of the carboxylic ionophores, lasalocid A and monensin, in aqueous solvent mixtures...
  14. REGULATION OF GLUT1 GENE EXPRESSION BY HYPOXIA
    Faramarz Ismail Beigi; Fiscal Year: 2001
    ..RNA binding factors mediate the observed stabilization of GLUT1 mRNA in cells treated with azide, calcium ionophores, and anisomycin...
  15. THE MOLECULAR BASIS OF ELECTRICAL EXCITABILITY
    William Catterall; Fiscal Year: 1980
    Na ion ionophores involved in action potential generation in electrically excitable cells have been found to have three separate receptor sites for toxins...
  16. PRODUCTION OF CYTOKINES BY ACTIVATED MAST CELLS
    Donald MacGlashan; Fiscal Year: 1992
    We were the first to demonstrate that stimulation of IL-3 dependent murine mast cell lines with calcium ionophores, or by crosslinking high affinity IgE receptors (FcepsilonRI) with antigen or anti- IgE, induces the production of IL-3, IL-..
  17. PATHOGENESIS OF DIARRHEA:PROTEIN KINASE C & SECRETAGOGUE
    CYNTHIA SEARS; Fiscal Year: 1993
    ..The roles of calcium and PKC activation in the synergy with STa will be examined by using calcium ionophores, phorbol ester, a diacylglycerol analogue, and inhibitors of PKC and intracellular calcium; and II...
  18. MECHANISMS OF ANGIOTENSIN II: CYCLIC NUCLEOTIDES & CA2+
    LARRY BARNES; Fiscal Year: 1980
    ..nucleotide levels as function of calcium concentration and in the presence and absence of calcium transport ionophores and blockers will indicate whether or not calcium participates in the mechanism...
  19. EFFECTS OF HYPERTHERMIA ON IMMUNE FUNCTIONS IN VITRO
    EDDIE LAMON; Fiscal Year: 1992
    ..These will include: ethanol and acetaldehyde, calcium and calcium ionophores, retinoids, indomethacin and two recombinant cytokines; IL-2 and Gamma Interferon...
  20. NEUROENDOCRINE REGULATION OF WATER BALANCE IN INSECTS
    JEFFREY SPRING; Fiscal Year: 1991
    ..which second messenger pathway (cAMP; calcium/calmodulin; diacylglycerol) using radioimmunoassay (cAMP), ionophores (calcium) and phorbol esters (diacylglycerol)...
  21. CONTROL OF POTASSIUM TRANSPORT IN BLADDER
    PHILIP STEINMETZ; Fiscal Year: 1980
    ..Since the luminal cell membrane is directly accessible to translocator molecules for potassium, either ionophores (valinomycin) or channel forming agents (nystatin, gramicidin), the effects of such translocators on ..
  22. MECHANISMS OF PH REGULATION OF LUNG SURFACTANT SECRETION
    Avinash Chander; Fiscal Year: 1992
    ..isolated type II cells, and isolated plasma membranes on stimulation of secretion with alkalosis, ATP, calcium ionophores, and terbutaline...
  23. ROLE OF ENDOGENOUS LEUKOTRIENE PRODUCTION IN LYMPHOCYTE
    DURGAPRASADARAO ATLURU; Fiscal Year: 1991
    ..T cells will be stimulated by a variety of compounds including mitogens, antigens, calcium ionophores, interleukin 1 (IL-1) and IL-2...
  24. REGULATION OF RECEPTOR MEDIATED ENDOCYTOSIS--HUMAN CELL
    Robert Fallon; Fiscal Year: 1990
    ..will be investigated using biochemical agents that perturb cell function (ligand, phorbol esters, calcium ionophores)...
  25. MECHANISMS OF PEPSINOGEN SECRETION FROM CHIEF CELLS
    Jean Pierre Raufman; Fiscal Year: 1990
    ..in cell calcium concentration play a role in mediating the actions of carbachol, cholecystokinin, and calcium ionophores. Although it has been known for several years that potentiation of pepsinogen secretion occurs when agents whose ..
  26. SYNTHESES OF MACROLIDES AND RELATED NATURAL PRODUCTS
    FREDERICK ZIEGLER; Fiscal Year: 1992
    Polypropionate-derived natural products can function as antibiotics, ionophores and pheromones...
  27. CALCIUM AND EPITHELIAL TRANSPORT
    Wadi Suki; Fiscal Year: 1990
    ..Also to be studied is the effect of increasing cytosolic calcium with ionophores, and inhibitors of mitochondrial Ca-ATPase and the Na/Ca antiport at the basolateral membrane...
  28. BINDING, TRANSPORT OF CALCIUM AND POTASSIUM IONOPHORES
    Duncan Haynes; Fiscal Year: 1980
    Three ionophores with intrinsic fluorescence signals will be studied by a combination of fluorescence-lifetime and stopped-flow rapid mixing techniques to gain information about their location within membranes and the rates and mechanisms ..
  29. TRANSPORT ACROSS CAPILLARY ENDOTHELIUM
    FITZ ROY CURRY; Fiscal Year: 2001
    ..They have found that in microvessels exposed to calcium ionophores, processes which depolarize the endothelial cell membrane reduce calcium influx and decrease microvessel ..
  30. LITHIUM-SELECTIVE ELECTRODES WITH POLYMERIC IONOPHORES
    Paul Hale; Fiscal Year: 1993
    ..Improved lithium ionophores would enable molecules that function as ion carriers in polymer membranes...
  31. QUASI-IRREVERSIBLE BLOCKADE OF NICOTINE'S CENTRAL ACTION
    Paul Clarke; Fiscal Year: 1991
    ..to nicotinic cholinoceptors (nAChRs); it may damage neurons, possibly entering via nAChR- associated ionophores; and it may inhibit the expression of nAChRs by some other mechanism...
  32. Manipulation of Lead Using Carboxylic Acid Ionophores
    Douglas Pfeiffer; Fiscal Year: 2005
    ..There may be additional grounds for concern in the case of antibiotic ionophores, which are added to the feed of nearly all species that are employed by this industry...
  33. CALCIUM AND CELL GROWTH
    Brian Herman; Fiscal Year: 1993
    ..by manipulation of the extracellular ionic environment or pharmacologic antagonists, through the use of Ca++ ionophores, caged Ca++ or caged Ca++ buffer, and by microinjection of BAPTA-conjugated dextran...
  34. Cellular Ion Control by Laser-Induced Permeabilization
    Imran Clark; Fiscal Year: 2004
    ..g., ionophores), release of intracellular calcium stores (by compounds or electrical stimulation) and electroporation...
  35. METABOLIC MODIFICATION OF REPAIR OF RADIATION DAMAGE
    MARIE VARNES; Fiscal Year: 1993
    ..5- 6.8). The mechanisms by which ionophores inhibit PLDR and increase heat toxicity will be studied as a function pHe...
  36. NON-IONIC LIPOSOMES AND REDOX ACTIVATED CATION PUMPS
    Luis Echegoyen; Fiscal Year: 1990
    In previous work, we have established that electrochemical switching of lariat ether-based ionophores can effect trans- membrane transport of various cations...
  37. BIOCHEMICAL REGULATION OF GENE ACTIVATION IN T CELLS
    Barbara Adams; Fiscal Year: 1991
    ..and the appearance of nuclear transcripts for IL-2 will be examined in cells treated with Ca+2 or H+ ionophores or cyclic nucleotide analogs to determine if changes in (Ca+2)i, pHi, and/or (cAMP)i can effect transcriptional ..
  38. TOTAL SYNTHESIS OF NATURAL CARDIOTONIC IONOPHORES
    DAVID WALBA; Fiscal Year: 1980
    ..Monensin is a member of the class of Streptomyces mold metabolites known as monocarboxylic acid ionophores. Members of this class of natural products possess interesting biological properties, including commercially ..
  39. THE SYNTHESIS OF SOME COMPLEX CYTOTOXIC COMPOUNDS
    RICHARD SCHLESSINGER; Fiscal Year: 1990
    ..Over the past year, we have developed, via a consideration of ionophores in general, new methods, based on the chemistry of vinylogous urethanes, to deal with the intricate ..
  40. AGING AND BRAIN ACETYLCHOLINE RELEASE
    EDWIN MEYER; Fiscal Year: 1993
    ..Does aging affect the potency of calcium ionophores with respect to protein kinase C translocation? 4...
  41. X-RAY DIFFRACTION STUDIES OF OLIGOPEPTIDES
    Isabella Karle; Fiscal Year: 2007
    ..widely explored in this laboratory in designed peptides, as well as in naturally occurring peptides such as the ionophores antiamoebin and zervamicin, during the current grant period...
  42. INITIATING MECHANISMS OF LIGHT DAMAGE IN THE RETINA
    Barry Winkler; Fiscal Year: 1992
    ..concentration of calcium and to determine the mechanism of this increase using calcium channel blockers, ionophores, and divalent ion substitutes; and 4) To determine whether rhodopsin bleaching is associated with increased ..
  43. GENETICS OF INVASION AND EGRESS IN TOXOPLASMA
    John Boothroyd; Fiscal Year: 2003
    ..For the egress studies, we have exploited the observation of others that calcium ionophores such as A23187 will induce egress when applied to infected cultures...
  44. MAMMALIAN CELL SURFACE GENETICS AND BIOCHEMISTRY
    Henry Wu; Fiscal Year: 1992
    ..and yeast mutants altered in N- glycosylation; (2) isolation and characterization of CHO mutants resistant to ionophores monensin and nigericin; (3) isolation and characterization of Vero cell mutants defective in diphtheria toxin ..
  45. PHTHALOCYANINE PHOTODYNAMIC THERAPY--MECHANISTIC STUDIES
    NANCY OLEINICK; Fiscal Year: 2002
    ..by examining (a) the activation of signal transduction pathways, (b) combined modality treatments of PDT with ionophores and polyamine inhibitors, (c) the induction of apoptosis by PDT in vitro and in vivo and by conditions thought ..
  46. CRANIOFACIAL MORPHOGENESIS IN PRENATAL ALCOHOL EXPOSURE
    Susan Smith; Fiscal Year: 2005
    ..The requirement for Ca2+ release is tested through the use of fluorescent Ca2+ indicators, Ca2+ ionophores and chelators, and the direct assay of downstream, Ca2+-dependent signaling proteins (CaM kinase, calcineurin)...
  47. SODIUM-CALCIUM INTERACTIONS IN MAMMALIAN MYOCARDIUM
    JOHN SUTKO; Fiscal Year: 1980
    ..Ion-selective ionophores, compounds capable of carrying ions across membranes, will be used to achieve controlled alterations of ..
  48. Design of Ca2+ Sensors to Monitor Ca2+ Signaling in ER
    Jenny Yang; Fiscal Year: 2005
    ..our developed Ca(ll) sensors in vivo by varying the intracellular cytosolic Ca(ll) concentration using Ca(ll) ionophores and appropriate Ca(ll) pump inhibitors, and comparing the estimated Ca(ll) concentrations with that derived ..
  49. ANALYSIS OF BASOLATERAL ION CHANNELS IN SKIN
    J Garcia Diaz; Fiscal Year: 1990
    ..Other experiments in intact epithelia treated with ionophores will characterize the macroscopic K current so that it will be possible to identify the channels responsible for ..
  50. COMBINATORIAL APPROACH TO ARTIFICIAL RECEPTOR DESIGN
    Andrew Hamilton; Fiscal Year: 1999
    ..We anticipate that the receptor library strategy can be extended to the formation metal templated ionophores that change their colorimetric or fluorescence properties on binding to target metal ions...
  51. 'Optical Nanosensors for Subcellular Chemical Imaging'
    Raoul Kopelman; Fiscal Year: 2001
    ..in a variety of biocompatible matrices, the suggested methodologies will use, in tandem, optically silent ionophores and enzymes, so as to apply these sensors to a wide range of intracellular ions, free radicals and molecules, ..
  52. CARRIERS AND CHANNELS IN BIOLOGICAL SYSTEMS
    Adil Shamoo; Fiscal Year: 1980
    ..serve either as carriers or channels in artificial membranes were either from microbial organisms (antibiotic ionophores) or synthetic compounds...
  53. IONOPHORE-BASED CHEMICAL ION SENSORS
    Eric Bakker; Fiscal Year: 2001
    This research develops novel, general, and convenient methodologies to characterize ionophores and sensor materials relevant to optical, potentiometric and voltammetric transduction schemes...
  54. Nanometer sized sensors with biological transducers
    CRAIG ASPINWALL; Fiscal Year: 2007
    ..Though quite useful for a variety of ions and small molecules where fluorescent indicators or ionophores are available, large categories of chemically and biologically relevant analytes elude detection due to a lack ..
  55. CYTOSOLIC CA2+IONOPHORES FOR FLUORESCENCE AND L9F NMR
    MARGARET LANGMUIR; Fiscal Year: 1992
    ..It can be used for single cell imaging, flow cytometry, cell function studies and for NRM study of perfused organs...
  56. PLATELET CA2+ HOMEOSTASIS IN ESSENTIAL HYPERTENSION
    Abraham Aviv; Fiscal Year: 1992
    ..experiments in the presence and absence of extracellular Ca, in the presence of Ca channel blockers and Ca ionophores, and after the quenching of cellular Ca...
  57. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
    ....
  58. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  59. ION CHANNEL REGULATION BY THE CYTOPLASMIC TAIL OF PDK1
    Seth Alper; Fiscal Year: 2005
    ..We will search for novel interacting proteins, and prepare protein suitable for structural analysis. ..
  60. Nociception in Diabetic Neuropathy: Role of Endothelins
    Liliana Berti Mattera; Fiscal Year: 2004
    ..abstract_text> ..
  61. Animal model for inhalant abuse
    Andrew Gifford; Fiscal Year: 2004
    ..Additionally, an animal model for inhalant abuse will aid further exploration of the factors contributing to the predisposition, development and potential treatment of inhalant abuse. ..
  62. Topical Treatment for Mild Psoriasis
    Wendy Bollag; Fiscal Year: 2007
    ..An effective, easy-to-use, low-cost therapy suitable for long-term relief would be a great boon. [unreadable] [unreadable] [unreadable] [unreadable]..