blood substitutes

Summary

Summary: Substances that can carry oxygen to and carbon dioxide away from the tissues when introduced into the blood stream. They are used to replace hemoglobin in severe hemorrhage and also to perfuse isolated organs. The best known are perfluorocarbon emulsions and various hemoglobin solutions.

Top Publications

  1. ncbi Effects of recombinant-hemoglobin solutions rHb2.0 and rHb1.1 on blood pressure, intestinal blood flow, and gut oxygenation in a rat model of hemorrhagic shock
    Nicolaas J H Raat
    Department of Physiology, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    J Lab Clin Med 145:21-32. 2005
  2. ncbi Bone-forming capacity of mesenchymal stromal cells when cultured in the presence of human platelet lysate as substitute for fetal bovine serum
    Henk Jan Prins
    Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Tissue Eng Part A 15:3741-51. 2009
  3. ncbi First-generation blood substitutes: what have we learned? Biochemical and physiological perspectives
    Abdu I Alayash
    Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biochemistry and Vascular Biology, Division of Hematology, National Institutes of Health Campus, Bethesda, MD 20892, USA
    Expert Opin Biol Ther 7:665-75. 2007
  4. pmc Blood substitutes. Artificial oxygen carriers: perfluorocarbon emulsions
    D R Spahn
    Institut für Anästhesiologie, Universitatsspital, Zurich, Switzerland
    Crit Care 3:R93-7. 1999
  5. ncbi Fluorinated materials for in vivo oxygen transport (blood substitutes), diagnosis and drug delivery
    J G Riess
    MRI Institute, Medical Center, University of California, San Diego 92103 1990, USA
    Biomaterials 19:1529-39. 1998
  6. pmc Blood substitutes. Haemoglobin therapeutics in clinical practice
    J F Baron
    Hopital Broussais, Paris, France
    Crit Care 3:R99-102. 1999
  7. pmc Tyrosine residues as redox cofactors in human hemoglobin: implications for engineering nontoxic blood substitutes
    Brandon J Reeder
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    J Biol Chem 283:30780-7. 2008
  8. ncbi In vitro macrophage uptake and in vivo biodistribution of PLA-PEG nanoparticles loaded with hemoglobin as blood substitutes: effect of PEG content
    Yan Sheng
    The State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Mailbox 112, 130 Meilong Road, Xuhui District, Shanghai, 200237, People s Republic of China
    J Mater Sci Mater Med 20:1881-91. 2009
  9. ncbi Setbacks in blood substitutes research and development: a biochemical perspective
    Abdu I Alayash
    Division of Hematology, Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, 8800 Rockville Pike, Bethesda, MD 20892, USA
    Clin Lab Med 30:381-9. 2010
  10. ncbi HBOC-201 as an alternative to blood transfusion: efficacy and safety evaluation in a multicenter phase III trial in elective orthopedic surgery
    Jonathan S Jahr
    David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Trauma 64:1484-97. 2008

Detail Information

Publications259 found, 100 shown here

  1. ncbi Effects of recombinant-hemoglobin solutions rHb2.0 and rHb1.1 on blood pressure, intestinal blood flow, and gut oxygenation in a rat model of hemorrhagic shock
    Nicolaas J H Raat
    Department of Physiology, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    J Lab Clin Med 145:21-32. 2005
    ..0, the effect of rHb2.0 on pH, base-excess and microvascular Po2 levels after resuscitation were comparable to those achieved with the use of the albumin solution...
  2. ncbi Bone-forming capacity of mesenchymal stromal cells when cultured in the presence of human platelet lysate as substitute for fetal bovine serum
    Henk Jan Prins
    Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Tissue Eng Part A 15:3741-51. 2009
    ..These results warrant the use of PL for ex vivo expansion of human MSCs for bone tissue engineering applications...
  3. ncbi First-generation blood substitutes: what have we learned? Biochemical and physiological perspectives
    Abdu I Alayash
    Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biochemistry and Vascular Biology, Division of Hematology, National Institutes of Health Campus, Bethesda, MD 20892, USA
    Expert Opin Biol Ther 7:665-75. 2007
    ..or recombinant hemoglobin (Hb)-based oxygen carriers (HBOCs) have been developed as oxygen therapeutics or 'blood substitutes' for use in a variety of clinical settings...
  4. pmc Blood substitutes. Artificial oxygen carriers: perfluorocarbon emulsions
    D R Spahn
    Institut für Anästhesiologie, Universitatsspital, Zurich, Switzerland
    Crit Care 3:R93-7. 1999
    ....
  5. ncbi Fluorinated materials for in vivo oxygen transport (blood substitutes), diagnosis and drug delivery
    J G Riess
    MRI Institute, Medical Center, University of California, San Diego 92103 1990, USA
    Biomaterials 19:1529-39. 1998
    ..Fluorinated amphiphiles also allowed the obtaining of a variety of stable reverse and multiple emulsions and gels. These systems are being investigated as drug delivery devices...
  6. pmc Blood substitutes. Haemoglobin therapeutics in clinical practice
    J F Baron
    Hopital Broussais, Paris, France
    Crit Care 3:R99-102. 1999
    ....
  7. pmc Tyrosine residues as redox cofactors in human hemoglobin: implications for engineering nontoxic blood substitutes
    Brandon J Reeder
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    J Biol Chem 283:30780-7. 2008
    ..Ferryl iron has been suggested to be a major cause of the oxidative toxicity of hemoglobin-based blood substitutes. Engineering hemoglobin with enhanced rates of ferryl removal, as we show here, is therefore likely to ..
  8. ncbi In vitro macrophage uptake and in vivo biodistribution of PLA-PEG nanoparticles loaded with hemoglobin as blood substitutes: effect of PEG content
    Yan Sheng
    The State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Mailbox 112, 130 Meilong Road, Xuhui District, Shanghai, 200237, People s Republic of China
    J Mater Sci Mater Med 20:1881-91. 2009
    ..of poly(lactic acid) (PLA)-poly(ethylene glycol) (PEG) hemoglobin (Hb)-loaded nanoparticles (HbP) used as blood substitutes. The HbP were prepared from PLA and PLA-PEG copolymer of varying PEG contents (5, 10, and 20 wt%) by a ..
  9. ncbi Setbacks in blood substitutes research and development: a biochemical perspective
    Abdu I Alayash
    Division of Hematology, Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, 8800 Rockville Pike, Bethesda, MD 20892, USA
    Clin Lab Med 30:381-9. 2010
    Recent setbacks in using Hb-based technology to develop oxygen carriers or blood substitutes may spur new and fundamentally different approaches for the development of a new generation of hemoglobin-based oxygen carriers (HBOCs)...
  10. ncbi HBOC-201 as an alternative to blood transfusion: efficacy and safety evaluation in a multicenter phase III trial in elective orthopedic surgery
    Jonathan S Jahr
    David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Trauma 64:1484-97. 2008
    ..The ability of hemoglobin based oxygen carrier-201 (HBOC-201) to safely reduce and/or eliminate perioperative transfusion was studied in orthopedic surgery patients...
  11. ncbi Low-volume fluid resuscitation for presumed hemorrhagic shock: helpful or harmful?
    S A Stern
    Department of Emergency Medicine, University of Michigan, Ann Arbor, Michigan 48109 0303, USA
    Curr Opin Crit Care 7:422-30. 2001
    ..Future research should focus on developing resuscitation methods that may actually enhance tissue perfusion during limited resuscitation and therefore offset its potential detrimental effects...
  12. ncbi Resuscitation from hemorrhagic shock with HBOC-201 in the setting of traumatic brain injury
    Jeffrey D Kerby
    Department of Surgery, Section of Trauma Burns and Surgical Critical Care, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Shock 27:652-6. 2007
    ..These results suggest that resuscitation with HBOC-201 protects autoregulatory mechanisms and may reduce secondary brain injury in traumatic brain injury...
  13. ncbi DCL-Hb for trauma patients with severe hemorrhagic shock: the European "On-Scene" multicenter study
    Thoralf Kerner
    Klinik fur Anasthesiologie und Operative Intensivmedizin, Campus Virchow Klinikum, Charite, Humboldt Universitat, Augustenburger Platz 1, 13353 Berlin, Germany
    Intensive Care Med 29:378-85. 2003
    ..Early administration of an oxygen carrier may reduce the occurrence of organ failures and improve survival. It may also reduce the need of blood products...
  14. ncbi Molecular aspects of the high oxygen affinity of non-hypertensive hexa pegylated hemoglobin, [(SP-PEG5K)(6)-Hb]
    Dongxia Li
    Department of Physiology, Albert Einstein College of Medicine, Bronx, NY, USA
    Artif Cells Blood Substit Immobil Biotechnol 35:19-29. 2007
    ....
  15. ncbi Model of nitric oxide diffusion in an arteriole: impact of hemoglobin-based blood substitutes
    Mahendra Kavdia
    Department of Biomedical Engineering and Center for Computational Medicine and Biology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205, USA
    Am J Physiol Heart Circ Physiol 282:H2245-53. 2002
    ..The SM NO concentration was significantly reduced when the extravasation of HBOC molecules was considered. The myoglobin present in the parenchymal cells scavenges NO, which reduces the SM NO concentration...
  16. pmc Recombinant hemoglobins as artificial oxygen carriers
    Clara Fronticelli
    Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Artif Cells Blood Substit Immobil Biotechnol 35:45-52. 2007
    ....
  17. ncbi Hb(alphaalpha,betabeta): a novel fusion construct for a dimeric, four-domain hemoglobin
    Gianna Panetta
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita di Roma Sapienza, Piazzale A Moro 5 00185 Roma, Italy
    Biochim Biophys Acta 1784:1462-70. 2008
    Hemoglobin-based blood substitutes are one of the options available to derive a resuscitating fluid taking into account clinical and physiological demands...
  18. ncbi Use of hemoglobin-based oxygen-carrying solution-201 to improve resuscitation parameters and prevent secondary brain injury in a swine model of traumatic brain injury and hemorrhage: laboratory investigation
    Guy Rosenthal
    Department of Neurosurgery, University of California, San Francisco, CA 94117, USA
    J Neurosurg 108:575-87. 2008
    ..We studied the effects of hemoglobin-based oxygen-carrying (HBOC)-201 solution compared with lactated Ringer (LR) solution in a large animal model of brain injury and hemorrhage, in a blinded prospective randomized study...
  19. ncbi Towards a novel haemoglobin-based oxygen carrier: Euro-PEG-Hb, physico-chemical properties, vasoactivity and renal filtration
    I Portöro
    Institute of Human Physiology and Clinical Experimental Research, Faculty of Medicine, Semmelweis University, Budapest, Hungary
    Biochim Biophys Acta 1784:1402-9. 2008
    ....
  20. ncbi Cell-free oxygen carriers: scientific foundations, clinical development, and new directions
    Robert M Winslow
    Sangart Inc, San Diego, California, USA
    Biochim Biophys Acta 1784:1382-6. 2008
    ..Because of its low P50 and increased molecular size, the release of O2 in resistance vessels (arterioles) by Hemospan is restricted, and vasoconstriction is greatly reduced...
  21. ncbi A recombinant polymeric hemoglobin with conformational, functional, and physiological characteristics of an in vivo O2 transporter
    Kevin M Bobofchak
    Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, 600 N Wolfe St, Baltimore, MD 21287, USA
    Am J Physiol Heart Circ Physiol 285:H549-61. 2003
    ..Polymerization maintains physiologically relevant oxygen and heme affinity, stability toward denaturation and oxidation, and effective oxygen delivery as indicated by reduced cerebral ischemic damage...
  22. ncbi Ligand reactivity and allosteric regulation of hemoglobin-based oxygen carriers
    Luca Ronda
    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy
    Biochim Biophys Acta 1784:1365-77. 2008
    ....
  23. ncbi Design of recombinant hemoglobins for use in transfusion fluids
    Clara Fronticelli
    Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Blalock, Baltimore, MD 21287, USA
    Crit Care Clin 25:357-71, Table of Contents. 2009
    ..These polymers of recombinant hemoglobin have the characteristics of molecular size, molecular stability, and oxygen delivery to hypoxic tissue suitable for an HBOC...
  24. ncbi Liposome-encapsulated actin-hemoglobin (LEAcHb) artificial blood substitutes
    Shuliang Li
    Department of Chemical and Biomolecular Engineering, University of Notre Dame, 171 Fitzpatrick Hall, Notre Dame, IN 46556, USA
    Biomaterials 26:3759-69. 2005
    ..has been applied to develop liposome-encapsulated actin-hemoglobin (LEAcHb) dispersions as potential blood substitutes by introducing an actin matrix into the liposome aqueous core...
  25. ncbi The risks of blood transfusions and the shortage of supply leads to the quest for blood substitutes
    Nicole Soraya Nouwairi
    Virginia Commonwealth University, Department of Nurse Anesthesia, Richmond, USA
    AANA J 72:359-64. 2004
    A number of factors have combined to drive the interest in developing blood substitutes. These include the time-dependent decrement in stored blood biochemistry, the general shortage of the blood supply, and public awareness of the risks ..
  26. ncbi PEGylation promotes hemoglobin tetramer dissociation
    Dario Caccia
    Dipartimento di Scienze e Tecnologie Biomediche, Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Universita degli Studi di Milano, Milano, Italy
    Bioconjug Chem 20:1356-66. 2009
    ..These features are more pronounced for PEG-Hb(oxy) than PEG-Hb(deoxy). The detected heterogeneity might be a source of adverse effects when PEGylated Hbs are used as blood substitutes.
  27. pmc A review of blood substitutes: examining the history, clinical trial results, and ethics of hemoglobin-based oxygen carriers
    Jiin Yu Chen
    Anesthesiology, University of Texas Medical Branch, Galveston, TX, USA
    Clinics (Sao Paulo) 64:803-13. 2009
    ..Thus, the available evidence suggests that HemAssist, Polyheme, and Hemopure are associated with a significant level of cardiovascular dysfunction. The next generation of HBOCs remains under development...
  28. ncbi Electrophoretic analysis of PEGylated hemoglobin-based blood substitutes
    Luca Ronda
    Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di Parma, Italy
    Anal Biochem 408:118-23. 2011
    Polyethylene glycol (PEG)-conjugated hemoglobins, a novel class of blood substitutes, were investigated by a combination of native and denaturing one- and two-dimensional polyacrylamide gel electrophoresis (PAGE) coupled with the ..
  29. ncbi Modulation of expression and polymerization of hemoglobin Polytaur, a potential blood substitute
    Serena Faggiano
    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy
    Arch Biochem Biophys 505:42-7. 2011
    ....
  30. ncbi Insights from studies of blood substitutes in trauma
    Ernest E Moore
    Department of Surgery, Denver Health Medical Center and University of Colorado Health Sciences Center, Denver, Colorado 80204, USA
    Shock 24:197-205. 2005
    Most authorities believe that the greatest need for blood substitutes is in patients with unanticipated acute blood loss, and trauma is the most likely scenario...
  31. ncbi A prospective trial of diaspirin cross-linked hemoglobin solution in patients after elective repair of abdominal aortic aneurysm
    Eric L Bloomfield
    Division of Anesthesiology and Critical Care Medicine, Cleveland Clinic Foundation, Cleveland, OH, USA
    Mil Med 169:546-50. 2004
    ..However, its use may lead to decreases in cardiac output because of increases in afterload, which may pose serious problems with left ventricular function...
  32. ncbi Resuscitation from severe hemorrhagic shock after traumatic brain injury using saline, shed blood, or a blood substitute
    Jeffrey B Gibson
    Department of Surgery, University of Tennessee Health Science Center, Memphis 38163, USA
    Shock 17:234-44. 2002
    ..Further investigations in similar experimental models might provide some plausible explanations why DCLHb unexpectedly increased mortality in patients...
  33. ncbi The clinical trials of diaspirin cross-linked hemoglobin (DCLHb) in severe traumatic hemorrhagic shock: the tale of two continents
    Edward P Sloan
    Intensive Care Med 29:347-9. 2003
  34. ncbi The use of bovine hemoglobin glutamer-250 (Hemopure) in surgical patients: results of a multicenter, randomized, single-blinded trial
    Juraj Sprung
    Department of Anesthesiology and Vascular Surgery, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Anesth Analg 94:799-808, table of contents. 2002
    ..The administration of HBOC-201 was associated with a delayed (third postoperative day) dose-dependent increase in the plasma methemoglobin concentration. We conclude that the intraoperative use of HBOC-201 was generally well tolerated...
  35. ncbi Diaspirin-crosslinked hemoglobin reduces blood transfusion in noncardiac surgery: a multicenter, randomized, controlled, double-blinded trial
    Armin Schubert
    Department of General Anesthesiology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Anesth Analg 97:323-32, table of contents. 2003
    ..Whereas the side-effect profile of modified hemoglobin solutions needs to be improved, our data show that hemoglobin solutions can be effective at reducing exposure to allogeneic blood for elective surgery...
  36. pmc Recommendations on the use of recombinant activated factor VII as an adjunctive treatment for massive bleeding--a European perspective
    Jean Louis Vincent
    Department of Intensive Care, Erasme Hospital, Free University of Brussels, B 1070 Brussels, Belgium
    Crit Care 10:R120. 2006
    ..Our aim was to develop consensus guidelines for use of recombinant activated factor VII (rFVIIa) in massive hemorrhage...
  37. ncbi Current status of oxygen carriers ('blood substitutes'): 2006
    R M Winslow
    Sangart, Inc, San Diego, USA
    Vox Sang 91:102-10. 2006
    ..Such products may seem counterintuitive by traditional standards, but it is hoped that they will be less toxic, more beneficial to patients, and more economical to produce...
  38. ncbi The life-sustaining capacity of human polymerized hemoglobin when red cells might be unavailable
    Steven A Gould
    Northfield Laboratories Inc, Evanston, IL, USA
    J Am Coll Surg 195:445-52; discussion 452-5. 2002
    ..PolyHeme should be particularly useful when red cells may be temporarily unavailable. This article assesses survival at life-threatening RBC hemoglobin concentration ([Hb]) in massively bleeding patients who do not receive red cells...
  39. pmc Salvage of focal cerebral ischemic damage by transfusion of high O2-affinity recombinant hemoglobin polymers in mouse
    Masaaki Nemoto
    Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    J Appl Physiol 100:1688-91. 2006
    ..The assumption that the P50 of blood substitutes should be similar to that of blood might not be warranted when used during ischemic conditions.
  40. ncbi Choice of fluid influences outcome in prolonged hypotensive resuscitation after hemorrhage in awake rats
    Michael T Handrigan
    Division of Military Casualty Research, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Shock 23:337-43. 2005
    ..4x EBV), survival was no better than C and it resulted in the most significant acidosis. These results support the decision to use Hextend for Hypo, a strategy currently being applied on the battlefield...
  41. ncbi Present status of modified hemoglobin as blood substitutes and oral therapy for end stage renal failure using artificial cells containing genetically engineered cells
    T M Chang
    Faculty of Medicine, McGill University, Montreal, Quebec, Canada
    Ann N Y Acad Sci 944:362-72. 2001
    Artificial cell or bioencapsulation has been developed for use in bioartificial organs, drug delivery, blood substitutes, and other areas...
  42. ncbi Prehospital HBOC-201 after traumatic brain injury and hemorrhagic shock in swine
    Mayur B Patel
    Division of Trauma, DeWitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    J Trauma 61:46-56. 2006
    ..This study evaluates neurotoxicity, vasoactivity, cardiac toxicity, and inflammatory activity of HBOC-201 (Biopure, Cambridge, Mass.) resuscitation in a TBI model...
  43. ncbi Cell-free hemoglobin-based blood substitutes and risk of myocardial infarction and death: a meta-analysis
    Charles Natanson
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    JAMA 299:2304-12. 2008
    Hemoglobin-based blood substitutes (HBBSs) are infusible oxygen-carrying liquids that have long shelf lives, have no need for refrigeration or cross-matching, and are ideal for treating hemorrhagic shock in remote settings...
  44. ncbi Resuscitation with the hemoglobin-based oxygen carrier, HBOC-201, in a swine model of severe uncontrolled hemorrhage and traumatic brain injury
    Susan Stern
    Department of Emergency Medicine, University of Michigan, Ann Arbor, Michigan 48109 0303, USA
    Shock 31:64-79. 2009
    ....
  45. ncbi Biochemical aspects of the reaction of hemoglobin and NO: implications for Hb-based blood substitutes
    R P Patel
    Center for Free Radical Biology and Department of Pathology, Molecular and Cellular Division, University of Alabama at Birmingham, Birmingham, AL 35294 0019, USA
    Free Radic Biol Med 28:1518-25. 2000
    ..This can occur during hemolysis or administration of Hb-based blood substitutes. Scavenging of NO is a significant problem that limits the use of Hb-based blood substitutes in the clinic, ..
  46. pmc Blood conservation strategies to reduce the need for red blood cell transfusion in critically ill patients
    Alan T Tinmouth
    University of Ottawa Centre for Transfusion Research, Ottawa Health Research Institute, Ottawa, Ont
    CMAJ 178:49-57. 2008
    ..Further research is needed to determine the potential roles for other blood conservation strategies...
  47. ncbi Controlled safety study of a hemoglobin-based oxygen carrier, DCLHb, in acute ischemic stroke
    R Saxena
    Department of Neurology, University Hospital Dijkzigt, Rotterdam, The Netherlands
    Stroke 30:993-6. 1999
    ..In animal stroke models its use led to a significant reduction in the extent of brain injury. The primary objective of this study was to evaluate the safety of DCLHb in patients with acute ischemic stroke...
  48. ncbi Hemoglobin-based oxygen carrying compound-201 as salvage therapy for severe neuro- and polytrauma (Injury Severity Score = 27-41)
    Michael Dudkiewicz
    DeWitt Daughtry Family Department of Surgery, Division of Trauma, University of Miami Miller School of Medicine, Miami, FL, USA
    Crit Care Med 36:2838-48. 2008
    ..We tested the hypothesis that HBOC-201 would improve cerebrovascular resuscitation in a unique polytrauma model...
  49. ncbi Diaspirin cross-linked hemoglobin (DCLHb) in the treatment of severe traumatic hemorrhagic shock: a randomized controlled efficacy trial
    E P Sloan
    Department of Emergency Medicine, University of Illinois at Chicago, 60612, USA
    JAMA 282:1857-64. 1999
    ..Severe, uncompensated, traumatic hemorrhagic shock causes significant morbidity and mortality, but resuscitation with an oxygen-carrying fluid might improve patient outcomes...
  50. ncbi Randomized trial of diaspirin cross-linked hemoglobin solution as an alternative to blood transfusion after cardiac surgery. The DCLHb Cardiac Surgery Trial Collaborative Group
    M L Lamy
    Department of Anesthesia and Intensive Care Medicine of the University of Liège, Centre Hospitalier Universitaire, Belgium
    Anesthesiology 92:646-56. 2000
    ..New oxygen-carrying compounds such as diaspirin cross-linked hemoglobin (DCLHb) could enable more patients to avoid allogeneic blood transfusion...
  51. ncbi Perflubron emulsion delays blood transfusions in orthopedic surgery. European Perflubron Emulsion Study Group
    D R Spahn
    Department of Anesthesiology, University Hospital Zurich, Switzerland
    Anesthesiology 91:1195-208. 1999
    ..The aim of the present study is to compare the effectiveness of perflubron emulsion with the effectiveness of autologous blood or colloid infusion for reversal of physiologic transfusion triggers...
  52. ncbi An open letter to institutional review boards considering Northfield Laboratories' PolyHeme trial
    Ken Kipnis
    University of Hawaii, Manoa, USA
    Am J Bioeth 6:18-21. 2006
    ..Blood-the standard treatment for hemorrhagic shock-is not available in ambulances but is available in hospitals. The authors argue that the in-hospital stage of the study fails to meet ethical and regulatory standards...
  53. ncbi A human recombinant haemoglobin designed for use as a blood substitute
    D Looker
    Somatogen Inc, Boulder, Colorado 80301
    Nature 356:258-60. 1992
    ..Fusion of the two alpha-globin subunits increases the half-life of this haemoglobin molecule in vivo by preventing its dissociation into alpha beta dimers and therefore also eliminates renal toxicity...
  54. ncbi Determination of size distribution and encapsulation efficiency of liposome-encapsulated hemoglobin blood substitutes using asymmetric flow field-flow fractionation coupled with multi-angle static light scattering
    Dian R Arifin
    Department of Chemical and Biomolecular Engineering, Center for Molecularly Engineered Materials, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Biotechnol Prog 19:1798-811. 2003
    ..25 mmHg and 2.49. These oxygen-binding properties indicate that LEHbs possess strong potential as artificial blood substitutes. In addition, the metHb levels in PB-LEHb dispersions are significantly low even in the absence of ..
  55. ncbi Hemoglobin-based blood substitutes: oxygen carriers, pressor agents, or oxidants?
    A I Alayash
    Laboratory of Plasma Derivatives, Center for Biologics Evaluation and Research, Food and Drug Administration, NIH Campus, Bethesda, MD 20892, USA
    Nat Biotechnol 17:545-9. 1999
    Hemoglobin-based blood substitutes are being developed as oxygen-carrying agents for the prevention of ischemic tissue damage and hypovolemic (low blood volume) shock...
  56. ncbi Hemoglobin-based blood substitutes and the hazards of blood radicals
    A I Alayash
    Laboratory of Plasma Derivatives, Center for Biologics Evaluation and Research, Food and Drug Administration, National Institutes of Health Campus, Bethesda, Maryland 20892, USA
    Free Radic Res 33:341-8. 2000
    ..This article reviews our current understanding of heme-mediated toxicities and some of the emerging protective strategies used to overcome hemoglobin side reactions...
  57. doi The future of clinical trials evaluating blood substitutes
    Dean A Fergusson
    JAMA 299:2324-6. 2008
  58. ncbi No scavenging and the hypertensive effect of hemoglobin-based blood substitutes
    John S Olson
    Department of Biochemistry and Cell Biology, W M Keck Center for Computational Biology, Rice University, Houston, TX 77005, USA
    Free Radic Biol Med 36:685-97. 2004
    ..vasoconstrictive, and the resultant hypertension is a significant side effect of most hemoglobin-based blood substitutes. The major cause of this blood pressure effect seems to be the high rate of NO dioxygenation by cell-free ..
  59. ncbi Human polymerized hemoglobin for the treatment of hemorrhagic shock when blood is unavailable: the USA multicenter trial
    Ernest E Moore
    Department of Surgery, Denver Health Medical Center, University of Colorado, Denver, CO, USA
    J Am Coll Surg 208:1-13. 2009
    ..This multicenter phase III trial was the first US study to assess survival of patients resuscitated with a hemoglobin-based oxygen carrier starting at the scene of injury...
  60. ncbi Cardiovascular and coronary physiology of acute isovolemic hemodilution: a review of nonoxygen-carrying and oxygen-carrying solutions
    D R Spahn
    Institut für Anästhesiologie, Universitatsspital Zurich, Switzerland
    Anesth Analg 78:1000-21. 1994
    ..However, with enhanced purification, chemical modification or microencapsulation of the hemoglobin molecule, vasoconstriction can be limited...
  61. ncbi Physiological properties of blood substitutes
    D R Spahn
    , , , , Switzerland
    News Physiol Sci 16:38-41. 2001
    b>Blood substitutes (modified hemoglobin solutions, perfluorocarbon emulsions) serve as artificial oxygen carriers and are alternatives to blood transfusions...
  62. ncbi Blood substitutes: where do we stand today?
    B T Kjellstrom
    Experimental Traumatology Unit, Swedish Defence Research Agency and Department of Surgery, Karolinska Institute at Soder Hospital, Stockholm, Sweden
    J Intern Med 253:495-7. 2003
  63. ncbi Blood substitutes as pharmacotherapies in clinical practice
    Jonathan S Jahr
    Department of Anesthesiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Curr Opin Anaesthesiol 20:325-30. 2007
    To discuss the development and current status of blood substitutes, including hemoglobin-based oxygen carriers (HBOCs) and perfluorocarbons...
  64. ncbi Hb-based oxygen carriers: are we there yet?
    Thomas J Reid
    Department of Blood Research, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Transfusion 43:280-7. 2003
  65. ncbi Blood substitutes: the future is now
    Ernest E Moore
    Department of Surgery, Denver Health Medical Center and University of Colorado Health Sciences Center, Denver, CO 80204, USA
    J Am Coll Surg 196:1-17. 2003
  66. ncbi Blood substitutes and experimental models of trauma
    Kenneth G Proctor
    Daughtry Family Department of Surgery, University of Miami School of Medicine, Florida 33106, USA
    J Trauma 54:S106-9. 2003
  67. ncbi Blood substitutes based on nanobiotechnology
    Thomas Ming Swi Chang
    Department of Physiology, Faculty of Medicine, McGill University, 3655 Drummond Street, Montreal, Quebec, H3G 1Y6, Canada
    Trends Biotechnol 24:372-7. 2006
    ..by concerns of potential infective agents in donated blood, commercial enterprises have attempted to develop blood substitutes since the 1900s. After several years of development, a few of the many leads are showing promise...
  68. ncbi Blood substitutes and oxygen therapeutics: an overview and current status
    Jonathan S Jahr
    Department of Anesthesiology, University of California at Los Angeles, Los Angeles, CA 90095 1778, USA
    Am J Ther 9:437-43. 2002
    This review article discusses the development and implementation of a number of blood substitutes, including hemoglobin-based oxygen carriers (HBOCs) and perfluorocarbons...
  69. ncbi Modified hemoglobin-based blood substitutes: crosslinked, recombinant and encapsulated hemoglobin
    T M Chang
    Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada
    Vox Sang 74:233-41. 1998
    ..A third generation hemoglobin-based blood substitute is based on microencapsulated hemoglobin, artificial red blood cells, that more closely resemble a complete red blood cell...
  70. ncbi A hemoglobin based oxygen carrier, bovine polymerized hemoglobin (HBOC-201) versus Hetastarch (HEX) in an uncontrolled liver injury hemorrhagic shock swine model with delayed evacuation
    Jennifer Gurney
    Walter Reed Army Medical Center J G, Washington, DC, USA
    J Trauma 57:726-38. 2004
    ..As HBOC-201 improves outcome in animals with hemorrhagic shock (HS), we compared HBOC-201 and HEX (used by U.S. military special operations forces) in a swine model of delayed evacuation and uncontrolled HS...
  71. ncbi Artificial oxygen carriers as red blood cell substitutes: a selected review and current status
    Hae won Kim
    Department of Surgery, Brown University Medical School, The Miriam Hospital, Providence, RI 02906, USA
    Artif Organs 28:813-28. 2004
    ..This article provides a short review of the basic principles, approaches, and current status of RCS development. Results of preclinical and clinical studies including recent Phase II/III clinical studies are discussed...
  72. ncbi Overcoming blood shortages: red blood cell and platelet substitutes and membrane modifications
    Richard J Davey
    New York Blood Center, 310 East 67th Street, New York, NY 10021, USA
    Curr Hematol Rep 3:92-6. 2004
    ..However, these new products will be a useful alternative for selected clinical applications and will lessen our dependence on our marginally adequate blood supply...
  73. ncbi Is hemoglobin in hemoglobin vesicles infused for isovolemic hemodilution necessary to improve oxygenation in critically ischemic hamster skin?
    Jan A Plock
    Department of Orthopedic, Plastic and Hand Surgery, Inselspital Univ Hospital, CH 3010 Berne, Switzerland
    Am J Physiol Heart Circ Physiol 289:H2624-31. 2005
    ..01). We conclude that the encapsulation of Hb was essential to attenuate hypoxia and subsequent cell death in the critically ischemic tissue. However, the effect was partly attributed to the rheological changes exerted by the vesicles...
  74. ncbi A hemoglobin-based oxygen carrier, bovine polymerized hemoglobin (HBOC-201) versus hetastarch (HEX) in a moderate severity hemorrhagic shock swine model with delayed evacuation
    Nora Philbin
    Naval Medical Research Center, Combat Casualty Care, 2N77 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Resuscitation 66:367-78. 2005
    ..To evaluate the efficacy of HBOC-201 for resuscitation of hemorrhagic shock in a swine model incorporating soft tissue injury and delayed evacuation...
  75. ncbi Chemically modified porcine hemoglobins and their biological properties
    Cai Jin
    College of Material and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
    Protein Pept Lett 11:353-60. 2004
    ..Somewhat beneficial effects were shown in the treatment of hemorrhagic shock where modified hemoglobin solutions were used as resuscitation fluids in the hemorrhagic shock Sprague-Dawley (SD) rats model...
  76. ncbi [Current aspects in red blood cell substitutes]
    Yanfeng Wang
    The Key Laboratory of Bioactive Materials, Nankai University, Ministry of Education, Tianjin 300071, China
    Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 21:490-4. 2004
    ..When they are available for use it is likely that development will accelerate and even better products will substantially alleviate the world-wide shortage of blood for transfusion...
  77. ncbi The next generation in shock resuscitation
    Frederick A Moore
    Department of Surgery, University of Texas Houston Medical School, Houston, TX 77030, USA
    Lancet 363:1988-96. 2004
    ....
  78. ncbi Quantification of cerebral hemoglobin as a function of oxygenation using near-infrared time-resolved spectroscopy in a piglet model of hypoxia
    Sonoko Ijichi
    Kagawa University, Department of Pediatrics, Faculty of Medicine, Mikicho 1750 1, Kitagun, Kagawa 761 0793, Japan
    J Biomed Opt 10:024026. 2005
    ..Therefore, abs of the head with BET by FC is more useful for estimation of the absolute values of oxyHb and deoxyHb of the piglet head...
  79. ncbi New generation of hemoglobin-based oxygen carriers evaluated for oxygenation of critically ischemic hamster flap tissue
    Claudio Contaldo
    Department of Orthopedic, Plastic, and Hand Surgery, Inselspital University Hospital, Berne, Switzerland
    Crit Care Med 33:806-12. 2005
    ....
  80. ncbi Update on alternative oxygen carriers
    J R Hess
    University of Maryland School of Medicine, Baltimore 21201, USA
    Vox Sang 87:132-5. 2004
  81. pmc A bloodless revolution. A growing interest in artificial blood substitutes has resulted in new products that could soon improve transfusion medicine
    Andrea Rinaldi
    EMBO Rep 6:705-8. 2005
  82. ncbi Prehospital hemoglobin-based oxygen carrier resuscitation attenuates postinjury acute lung injury
    Tomohiko Masuno
    Department of Surgery, Denver Health Medical Center, CO 80204, USA
    Surgery 138:335-41. 2005
    ..The purpose of this in vivo study is to simulate an existing multicenter prehospital trial of HBOC versus crystalloid to determine the effects in a controlled 2-event construct of postinjury multiple organ failure...
  83. ncbi Acellular haemoglobin attenuates hypoxia-inducible factor-1alpha (HIF-1alpha) and its target genes in haemodiluted rats
    Dominador J Manalo
    LBVB Laboratory of Biochemistry and Vascular Biology, Division of Hematology, CBER Center for Biologics Evaluation and Research, FDA Food and Drug Administration, NIH National Institutes of Health, Bethesda, MD 20892, USA
    Biochem J 414:461-9. 2008
    Hb (haemoglobin)-based blood substitutes represent a class of therapeutics designed to correct oxygen deficit under conditions of anaemia and traumatic blood loss...
  84. ncbi Bovine polymerized hemoglobin versus Hextend resuscitation in a swine model of severe controlled hemorrhagic shock with delay to definitive care
    Jennifer Rice
    Naval Medical Research Center, Silver Spring, MD 20910, USA
    Shock 26:302-10. 2006
    ..01). In this swine model of controlled hemorrhage with low-volume resuscitation and delayed definitive care, HBOC-201 pigs had improved hemodynamics, transcutaneous tissue oxygen tension, and transfusion avoidance compared with HEX...
  85. ncbi Oxygen therapeutics: oxygen delivery without blood
    Joanna L Stollings
    Hospital Pharmacy Services, Mayo Clinic, Rochester, Minnesota 55902, USA
    Pharmacotherapy 26:1453-64. 2006
    ....
  86. ncbi Effects of isovolemic resuscitation with hemoglobin-based oxygen carrier Hemoglobin glutamer-200 (bovine) on systemic and mesenteric perfusion and oxygenation in a canine model of hemorrhagic shock: a comparison with 6% hetastarch solution and shed blood
    B Driessen
    Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, PA 19348, USA
    Vet Anaesth Analg 33:368-80. 2006
    ..To study Hemoglobin glutamer-200 bovine (Hb-200), 6% hetastarch (HES) and shed whole blood (WB) resuscitation in canine hemorrhagic shock...
  87. ncbi Haemoglobin, oxygen carriers and perioperative organ perfusion
    Roman Kocian
    Institute of Anaesthesiology, University Hospital, Raemistrasse 100, 8091 Zurich, Switzerland
    Best Pract Res Clin Anaesthesiol 22:63-80. 2008
    ..Research into some of these compounds has been discontinued, while others have advanced into clinical phase III trials, but none has achieved market approval for Europe, US or Canada so far...
  88. ncbi Low-volume resuscitation cocktail extends survival after severe hemorrhagic shock
    Penny S Reynolds
    Departments of Emergency Medicine, Virginia Commonwealth University Reanimation Engineering Shock Center, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298 0401, USA
    Shock 28:45-52. 2007
    ..Experimental evaluation of any resuscitation therapy should account for the degree of preexisting physiological compromise before therapy is initiated...
  89. ncbi Cerebral oxygen delivery by liposome-encapsulated hemoglobin: a positron-emission tomographic evaluation in a rat model of hemorrhagic shock
    Vibhudutta Awasthi
    Department of Radiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
    J Appl Physiol (1985) 103:28-38. 2007
    ..suggest that 15O-PET technology can be successfully employed to evaluate potential oxygen carriers and blood substitutes and that LEH resuscitation in hemorrhage enhances oxygen delivery to the cerebral tissue and improves oxygen ..
  90. ncbi Alternatives to allogeneic blood transfusions
    Andreas Pape
    Clinic of Anoesthesiology, Intensive Care Medicine and Pain Management, J W Goethe University Hospital Frankfurt am Main, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany
    Best Pract Res Clin Anaesthesiol 21:221-39. 2007
    ..Nevertheless, to date no artificial oxygen carrier is available for routine clinical use, and further studies are needed to show the safety and efficacy of these substances...
  91. ncbi Resuscitation with recombinant hemoglobin rHb2.0 in a rodent model of hemorrhagic shock
    Joerg Hermann
    Institute for Surgical Research, Ludwig Maximilians University, Munich, Germany
    Anesthesiology 107:273-80. 2007
    ..This study compares resuscitation with a nonhemoglobin colloid and two recombinant hemoglobin solutions with wild-type and reduced nitric oxide-scavenging capacity...
  92. ncbi Effects of Diaspirin Crosslinked Hemoglobin (DCLHb) on microcirculation and local tissue pO2 of striated skin muscle following resuscitation from hemorrhagic shock
    Sven Hungerer
    Department for Surgery, Trauma Clinic Murnau, Murnau, Germany
    Artif Cells Blood Substit Immobil Biotechnol 34:455-71. 2006
    ..DCLHb effectively increases local tissue pO2 after resuscitation from hemorrhagic shock; however, not as effectively as 10 g% AUB...
  93. ncbi Coagulation patterns following haemoglobin-based oxygen carrier resuscitation in severe uncontrolled haemorrhagic shock in swine
    F Arnaud
    Trauma and Resuscitative Medicine Department, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
    Transfus Med 16:290-302. 2006
    ..In this severe uncontrolled HS model, successful resuscitation with HBOC produced haemodilutional coagulopathy less than or similar to that produced by resuscitation with HEX...
  94. ncbi Oxygen transport during hemodilution with a perfluorocarbon-based oxygen carrier: effect of altitude and hyperoxia
    Tatiana Gardeazábal
    Blood Substitutes Laboratory, Fundación Cardio Infantil, Carrera 1 Este No 19 40, Office ML817, University of Los Andes, PO Box 4976, Bogota, Colombia
    J Appl Physiol (1985) 105:588-94. 2008
    ..Therefore, the efficacy of PFCOCs at 2,600 m above sea level is reduced, and altitude must be taken into account when PFCOCs are used...
  95. pmc Balance between vasoconstriction and enhanced oxygen delivery
    Pedro Cabrales
    La Jolla Bioengineering Institute, Department of Bioengineering, University of California, San Diego, La Jolla, California 92037, USA
    Transfusion 48:2087-95. 2008
    ..Responses to facilitated O(2) transport, after exchange transfusion with PBH, were studied in the hamster window chamber model during acute extreme anemia to determine how PBH affects microvascular perfusion and tissue oxygenation...
  96. ncbi Enhanced central organ oxygenation after application of bovine cell-free hemoglobin HBOC-201
    Marc Freitag
    Department of Anaesthesiology, University Hospital Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Can J Anaesth 52:904-14. 2005
    ....
  97. ncbi Prolonged low-volume resuscitation with HBOC-201 in a large-animal survival model of controlled hemorrhage
    Colleen M Fitzpatrick
    Department of Surgery, Wilford Hall Medical Center, Lackland Air Force Base, Texas, USA
    J Trauma 59:273-81; discussion 281-3. 2005
    ..This study compared a hemoglobin-based oxygen carrier (HBOC-201; Hemopure) to Hextend when used to treat hemorrhagic shock in situations of delayed definitive care military operations...
  98. ncbi Artificial O2 carriers: status in 2005
    Donat R Spahn
    Department of Anesthesiology, University Hospital Lausanne, CH 1011 Lausanne, Switzerland
    Curr Pharm Des 11:4099-114. 2005
    ..The most advanced products are in clinical phase III trials but no product has achieved market approval yet in the US, Europe or Canada...
  99. ncbi Innate immune responses in Swine resuscitated from severe traumatic hemorrhagic shock with hemoglobin-based oxygen carrier-201
    C Hall
    Naval Medical Research Center, Combat Casualty Care Directorate, Silver Spring, Maryland 20910, USA
    Artif Cells Blood Substit Immobil Biotechnol 35:259-74. 2007
    ..There was a trend to higher plasma IL-10 in HBOC-201 and groups vs. Hextend. We conclude that in swine with severe controlled HS and soft tissue injury, immune responses are similar with resuscitation with HBOC-201 and Hextend...
  100. ncbi Fluid resuscitation with hemoglobin vesicles in a rabbit model of acute hemorrhagic shock
    Katsuyuki Terajima
    Department of Anesthesiology, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8603, Japan
    Shock 25:184-9. 2006
    ..HbV solution offers the advantages of systemic oxygenation without impairing microcirculation in the treatment of hemorrhagic shock...
  101. ncbi Hemoglobin-based oxygen carriers in trauma care: scientific rationale for the US multicenter prehosptial trial
    Ernest E Moore
    Department of Surgery, Denver Health Medical Center, and Department of Surgery, University of Colorado Health Sciences Center, Denver, CO 80204, USA
    World J Surg 30:1247-57. 2006
    The greatest need for blood substitutes worldwide is in patients with unanticipated acute blood loss, and trauma is the most likely scenario...

Research Grants62

  1. BIOENGINEERING DESIGN OF ARTIFICIAL BLOOD
    Marcos Intaglietta; Fiscal Year: 2011
    ..hemoglobin) overcomes the most significant hurdle to the development of modified hemoglobin-based blood substitutes, namely vasoconstriction...
  2. Biomimetic Approach to the Fabrication of Red Blood Cell Mimics
    Joseph M DeSimone; Fiscal Year: 2010
    ..and manufacture a blood substitute and at this time there are no currently approved products for use as blood substitutes in North America or Europe...
  3. Mechanisms of Hemoglobin Adaptation to Hypoxia in High-altitude Rodents
    Jay F Storz; Fiscal Year: 2012
    ..may be possible to replicate the mechanism with novel drug-based therapy, gene therapy, and hemoglobin-based blood substitutes. This highly interdisciplinary study will integrate the tools and theory of molecular population genetics, ..
  4. Development of PEG Hemoglobin blood substitute to treat hemorrhagic shock
    Abe Abuchowski; Fiscal Year: 2009
    ..to overcome many of these issues, efforts have been made to develop hemoglobin based oxygen carrier (HBOCs) blood substitutes which have advantages such as decreased chance of disease transmission, lack of a need for typing, and most ..
  5. Retrievable Blood Oxygen Carrier System
    Agnes E Ostafin; Fiscal Year: 2012
    Responding to ever increasing need for blood substitutes, scientists have developed two types of artificial oxygen carriers (AOC), polymeric hemoglobin and perfluorocarbon based AOC...
  6. Predicting Brain Recovery after Acute Hemorrhage and Resuscitation
    John J A Marota; Fiscal Year: 2010
    ..hemorrhage, as well as elucidate the impact and optimal timing of resuscitation with blood or artificial blood substitutes. These data would strongly support the application of this technology to assess and manage patients ..
  7. Design of Heme Protein-Based Blood Substitutes
    John S Olson; Fiscal Year: 2010
    ..We have used the mechanisms governing these properties to design second-generation extracellular rHb-based blood substitutes with more efficient O2 transport and little or no hypertensive side effect in pre-clinical animal studies...
  8. Molecular and Biological Activities of Alpha Hemoglobin Stabilizing Protein
    Mitchell J Weiss; Fiscal Year: 2012
    ..the role of AHSP in HbA synthesis may provide tools to optimize the manufacture of recombinant Hb-based blood substitutes. Finally, defining functional interactions between iron and AHSP could provide insights into the ..
  9. IRON AND OXYGEN CHEMISTRIES IN SIPUNCULAN BLOOD
    DONALD KURTZ; Fiscal Year: 1992
    ..applications which may arise from this work include new molecules for regulating redox chemistry of 02-carrying proteins and iron transport in human erythrocytes, and the use of hemerythrin as an oxygen carrier in blood substitutes.
  10. COMMUNICATION PATHWAYS IN CENTRAL CAVITY MODIFIED HBS
    Joel Friedman; Fiscal Year: 2000
    Many of the synthetic strategies to create hemoglobin based acellular blood substitutes, employ modifications that perturb the central cavity and extend the hydrodynamic volume...
  11. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 2004
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes.
  12. CONTROL OF 02 TRANSPORT IN HEART AND SKELETAL MUSCLES
    THOMAS GAYESKI; Fiscal Year: 1992
    ..will provide insight into the clinical risks and benefits of administering or withholding blood or blood substitutes. Transport reserves must accommodate spatial and temporal heterogeneities of red cell flux...
  13. NOVEL CLASS OF FLUOROCARBONS AS BLOOD SUBSTITUTES
    Gordhan Patel; Fiscal Year: 1990
    A novel class of fluorochemical are proposed as blood substitutes. The proposed compositions will be synthesized, purified, and characterized...
  14. PHYSICAL BASIS FOR DIAGNOSTIC 19F MR IMAGING
    Peter Joseph; Fiscal Year: 1990
    ..blood perfusion and oxygen tension in the brain using magnetic resonance imaging (MRI) of fluorinated blood substitutes (FBS), and to compare the results with those obtained using several techniques which have been proposed ..
  15. New Paradigms in the Design of Blood Substitutes
    Joel Friedman; Fiscal Year: 2006
    ..Manjula, AECOM) in amounts needed for Projects 2(NMR), 3(optical spectroscopy and ligand reactivity), 4(EPR) and 5(physiology). ..
  16. NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS
    RAMACHANDRA HOSMANE; Fiscal Year: 2002
    ..organic reagents (PORs) for chemically modifying cell-free human hemoglobins for use as oxygen-carrying blood substitutes in emergency transfusions...
  17. CONSTRUCTION OF CAGE AND RACK WASHING FACILITY
    JOSEPH HARDER; Fiscal Year: 1992
    ..and cancer chemoprevention, cancer chemotherapy, immunology and immunotoxicology, artificial blood substitutes, and AIDS therapeutics...
  18. MULTINUCLEAR MRI OF TUMORS IN ANIMALS
    Peter Joseph; Fiscal Year: 1991
    ..The proton images will be used for their anatomical detail. The fluorine images will be of fluorinated blood substitutes and will dilineate vascular anatomy and measure the vascular oxygen tension...
  19. MOLECULAR CONTROLS OF HEMOGLOBIN FUNCTION
    Celia Bonaventura; Fiscal Year: 2000
    Our working hypothesis is that successful Hb-based blood substitutes may be generate by cross linking and sulfhydryl modification, with fine-tuning achieved by site-directed mutagenesis that is guided by knowledge of the molecular ..
  20. Engineering H-NOX Domains for Therapeutic Oxygen Delivery
    EMILY WEINERT; Fiscal Year: 2009
    ..There is currently a need for blood substitutes because of shortages in the blood supply and potential infection spread through donated blood, especially in ..
  21. MOLECULAR FINGERPRINTING OF CROSS LINKED HEMOGLOBINS
    Chien Ho; Fiscal Year: 2000
    ..features of cross-linked hemoglobins (Hbs) that provide suitable properties as Hb-based oxygen carriers or blood substitutes. This information will help design "ideal" cross-linked Hbs with desired oxygen affinity, cooperativity, and ..
  22. HEMOGLOBIN UTILIZATION AND VIRULENCE OF MENINGOCOCCI
    Igor Stojiljkovic; Fiscal Year: 2002
    ..meningitidis. Finally, understanding Hb-iron acquisition systems may assist scientists in design of a new family of Hb-based blood substitutes that do not increase the risk of infection by Hb-utilizing bacteria.
  23. RENOVATION OF BIOCHEMISTRY FACILITY
    Scott Bass; Fiscal Year: 2002
    ..Research in this space will focus on the development of antineoplastic and antiviral drugs, mechanisms of carcinogenesis and oxygen carrying blood substitutes for emergency transfusions.
  24. OXYGEN INDEPENDENT INTERFERENCE FREE GLUCOSE SENSORS
    Joseph Wang; Fiscal Year: 2000
    ..The use of perfluorochemicals, often employed as blood substitutes due to their remarkable oxygen solubility, as a major component of the biocomposite electrode, should ..
  25. The function and regulation of alpha hemoglobin stabilizing protein (AHSP) in hem
    Mitchell Weiss; Fiscal Year: 2007
    ..Second, our studies may provide valuable details as to how AHSP can be exploited as a molecular chaperone to improve the manufacture of recombinant Hb-based artificial blood substitutes.
  26. LACTATE SENSORS FOR BIOENGINEERING & CARDIAC MONITORING
    Dale Baker; Fiscal Year: 2001
    ..The fully characterized sensor will then be used in studies of myocardial ischemia and in the evaluation of blood substitutes.
  27. MULTINUCLEAR IN VIVO MRI/MRS--DEVELOPMENT & APPLICATION
    Robert Mattrey; Fiscal Year: 1990
    ..19F imaging of perfluorocarbon blood substitutes is important, since 19F relaxation rates are strongly influenced by 02 tension...
  28. CHELATING AGENTS AS BLOOD SUBSTITUTES
    Jack Baldwin; Fiscal Year: 1980
    ..The development of new oxygen carriers, based on chelates, is in progress. These are to be tested in vivo as oxygen transport equivalents. Modified forms of human hemoglobin are also being synthesized and tested for the same purposes. ..
  29. Oxygen Carriers for Hypovolemic Circulatory Collapse
    Robert Winslow; Fiscal Year: 2007
    ..As such, the molecules we have produced are not "blood substitutes" per se, but a new class of therapeutic agent, effective in small volume, that "target" O2 to capillaries ..
  30. Bioengineering Methods to Study Blood Substitutes
    Anthony Cheung; Fiscal Year: 2004
    ..abstract_text> ..
  31. PORPHYRIN IONS AND RADICALS IN METABOLIC PROCESSES
    David Dolphin; Fiscal Year: 1991
    ..Water soluble hemes, capable or reversibly binding dioxygen will be studied as possible blood substitutes.
  32. Instrument to Measure Oxygen Affinity of Hemoglobin
    Kim Vandegriff; Fiscal Year: 2004
    ..abstract_text> ..
  33. PET Evaluation of Hemorrhagic Shock and Resuscitation
    Vibhudutta Awasthi; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  34. NITRIC OXIDE 'NO' AND THE KIDNEY
    Chris Baylis; Fiscal Year: 2003
    ..Finally, she will evaluate the validity of the use of plasma and urinary NOX and will use Electron Spin Resonance (ESR) spectroscopy as an alternative method of determining NO production in vivo. ..
  35. COLLOIDAL IRON-OXIDE NANOBEACONS FOR THERANOSTIC USE IN ATHEROSCLEROSIS
    Gregory M Lanza; Fiscal Year: 2010
    ..Demonstrate image-guided drug delivery of antiangiogenic therapy potential with ligand-targeted non-gadolinium nanoplatform in vivo. ..
  36. Mechanically Stable Blood Substitutes
    Andre Francis Palmer; Fiscal Year: 2010
    ..be engineered to create mechanically stable and shape changing vesicular dispersions for use as artificial blood substitutes. We postulate that control of liposome structure and mechanics can be engineered by varying liposome size ..
  37. EFFECTS OF AGE ON SIGNALING IN HUMAN OSTEOBLASTOGENESIS
    Julie Glowacki; Fiscal Year: 2010
    ....
  38. MOLECULAR ICE CONTROL FOR ENGINEERED TISSUES
    Michael Taylor; Fiscal Year: 2003
    ..The potential markets are enormous. Cryopreservation methods, which result in retained viability and function, will be licensed to the tissue engineering industry. ..
  39. TRANSFUSION TRIGGER EXTENSION BY PLASMA EXPANDERS
    Marcos Intaglietta; Fiscal Year: 2009
    ..Strategies for optimal follow up of plasma resuscitation and exchange with blood transfusion will be investigated. ..
  40. MECHANOSENSITIVE MODULATION OF AIRWAY CONTRACTION
    Chi Ming Hai; Fiscal Year: 2008
    ..Understanding the basic science of this cellular mechanism may lead to new methods for the treatment of airway diseases such as asthma. ..
  41. FUNCTIONAL ASPECTS OF OXYGEN DELIVERY
    Marcos Intaglietta; Fiscal Year: 2004
    ..Our research aims at advancing our understanding of tissue oxygenation and provides a new conceptual framework with which to analyze the ischemic process. ..
  42. RESEARCH TRAINING IN TRANSFUSION MEDICINE
    Jeffrey McCullough; Fiscal Year: 2003
    ..abstract_text> ..
  43. The Glycocalyx and Endothelial Barrier Regulation
    RANDAL DULL; Fiscal Year: 2005
    ..This K08 will provide the foundation for a research career dedicated to understanding the molecular mechanisms of endothelial barrier regulation. ..
  44. TISSUE ENGINEERING OF GROWING JOINTS
    Julie Glowacki; Fiscal Year: 2002
    ..In vivo fate of engineered joints will be assessed in our model of murine joint transplantation and will be further verified in the pig. ..
  45. Pancreas Transporter Development and Validation
    Michael Taylor; Fiscal Year: 2006
    ..This research program is supported by collaboration with three major clinical centers interested in validation of this perfusion technology for their future use. [unreadable] [unreadable]..
  46. Indiana and the Legacy of State and Local Eugenics 1907-2007
    William Schneider; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  47. Ischemia Reperfusion Injury in the Diabetic Heart
    Paul McDonagh; Fiscal Year: 2007
    ..The results will improve our understanding of the role of chronic and acute inflammation in I/R injury in the diabetic heart and suggest new, thoughtful approaches to treat this growing problem. [unreadable] [unreadable]..
  48. IMCD phosphodiesterase in pregnancy; role in ECFVE
    Chris Baylis; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  49. Effect of Aging & Vitamin D Status on Osteoblastogenesis
    Julie Glowacki; Fiscal Year: 2008
    ..abstract_text> ..
  50. Activation of clotting & cell adhesion: gas embolism
    David M Eckmann; Fiscal Year: 2010
    ..This work is the basis for neuroprotection in gas embolism-induced stroke, a persistent, growing health threat without treatment. ..