phosphatidylcholines

Summary

Summary: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.

Top Publications

  1. pmc Mass spectrometry images acylcarnitines, phosphatidylcholines, and sphingomyelin in MDA-MB-231 breast tumor models
    Kamila Chughtai
    FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands
    J Lipid Res 54:333-44. 2013
  2. ncbi Biomolecular simulations of membranes: physical properties from different force fields
    Shirley W I Siu
    Theoretical and Computational Membrane Biology, Center for Bioinformatics, Saarland University, P O Box 151150, 66041 Saarbrucken, Germany
    J Chem Phys 128:125103. 2008
  3. pmc Oxidized phospholipid inhibition of toll-like receptor (TLR) signaling is restricted to TLR2 and TLR4: roles for CD14, LPS-binding protein, and MD2 as targets for specificity of inhibition
    Clett Erridge
    Strathclyde Institute of Pharmacy and Biomedical Sciences, Univesity of Strathclyde, 204 George St, Glasgow G1 1XW, United Kingdom
    J Biol Chem 283:24748-59. 2008
  4. ncbi Phosphatidylcholine and choline homeostasis
    Zhaoyu Li
    Department of Biochemistry and the Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Lipid Res 49:1187-94. 2008
  5. ncbi High throughput lipidomic profiling of schizophrenia and bipolar disorder brain tissue reveals alterations of free fatty acids, phosphatidylcholines, and ceramides
    Emanuel Schwarz
    Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom
    J Proteome Res 7:4266-77. 2008
  6. ncbi The unfolded protein response is an important regulator of inflammatory genes in endothelial cells
    Peter S Gargalovic
    UCLA Department of Medicine, Los Angeles, CA 90095 1679, USA
    Arterioscler Thromb Vasc Biol 26:2490-6. 2006
  7. pmc CHARMM-GUI Membrane Builder for mixed bilayers and its application to yeast membranes
    Sunhwan Jo
    Department of Molecular Biosciences and Center for Bioinformatics, University of Kansas, Lawrence, Kansas 66047, USA
    Biophys J 97:50-8. 2009
  8. pmc Instability of cholesterol clusters in lipid bilayers and the cholesterol's Umbrella effect
    Jian Dai
    Department of Physics, Texas Tech University, Lubbock, Texas 79409, USA
    J Phys Chem B 114:840-8. 2010
  9. pmc Conformations of islet amyloid polypeptide monomers in a membrane environment: implications for fibril formation
    Mojie Duan
    Gustaf H Carlson School of Chemistry and Biochemistry, Clark University, Worcester, Massachusetts, United States of America
    PLoS ONE 7:e47150. 2012
  10. ncbi Polar head groups are important for barrier-protective effects of oxidized phospholipids on pulmonary endothelium
    Anna A Birukova
    Section of Pulmonary and Critical Medicine, Department of Medicine, Division of Biomedical Sciences, University of Chicago, 929 East 57th St, CIS Bldg, W410, Chicago, IL 60637, USA
    Am J Physiol Lung Cell Mol Physiol 292:L924-35. 2007

Detail Information

Publications354 found, 100 shown here

  1. pmc Mass spectrometry images acylcarnitines, phosphatidylcholines, and sphingomyelin in MDA-MB-231 breast tumor models
    Kamila Chughtai
    FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands
    J Lipid Res 54:333-44. 2013
    ..In this study, we performed multimodal imaging of acylcarnitines, phosphatidylcholines (PC), a lysophosphatidylcholine (LPC), and a sphingomyelin (SM) from different microenvironments of breast ..
  2. ncbi Biomolecular simulations of membranes: physical properties from different force fields
    Shirley W I Siu
    Theoretical and Computational Membrane Biology, Center for Bioinformatics, Saarland University, P O Box 151150, 66041 Saarbrucken, Germany
    J Chem Phys 128:125103. 2008
    ..Both Berger and GAFF underestimate the lipid self-diffusion. GAFF offers a consistent force field for the atomic scale simulation of biomembranes...
  3. pmc Oxidized phospholipid inhibition of toll-like receptor (TLR) signaling is restricted to TLR2 and TLR4: roles for CD14, LPS-binding protein, and MD2 as targets for specificity of inhibition
    Clett Erridge
    Strathclyde Institute of Pharmacy and Biomedical Sciences, Univesity of Strathclyde, 204 George St, Glasgow G1 1XW, United Kingdom
    J Biol Chem 283:24748-59. 2008
    ....
  4. ncbi Phosphatidylcholine and choline homeostasis
    Zhaoyu Li
    Department of Biochemistry and the Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Lipid Res 49:1187-94. 2008
    ..Recent studies indicate that choline is recycled in the liver and redistributed from kidney, lung, and intestine to liver and brain when choline supply is attenuated...
  5. ncbi High throughput lipidomic profiling of schizophrenia and bipolar disorder brain tissue reveals alterations of free fatty acids, phosphatidylcholines, and ceramides
    Emanuel Schwarz
    Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom
    J Proteome Res 7:4266-77. 2008
    ..Overall, our findings suggest that lipid abnormalities may be a disease intrinsic feature of both schizophrenia and bipolar disorder reflected by significant changes in the central nervous system as well as peripheral tissues...
  6. ncbi The unfolded protein response is an important regulator of inflammatory genes in endothelial cells
    Peter S Gargalovic
    UCLA Department of Medicine, Los Angeles, CA 90095 1679, USA
    Arterioscler Thromb Vasc Biol 26:2490-6. 2006
    ..The goal of our studies was to identify pathways mediating the induction of inflammatory genes by oxPAPC...
  7. pmc CHARMM-GUI Membrane Builder for mixed bilayers and its application to yeast membranes
    Sunhwan Jo
    Department of Molecular Biosciences and Center for Bioinformatics, University of Kansas, Lawrence, Kansas 66047, USA
    Biophys J 97:50-8. 2009
    ..The membrane(sat) simulations were in a liquid-ordered state, and agree with similar experimental cholesterol-containing membranes...
  8. pmc Instability of cholesterol clusters in lipid bilayers and the cholesterol's Umbrella effect
    Jian Dai
    Department of Physics, Texas Tech University, Lubbock, Texas 79409, USA
    J Phys Chem B 114:840-8. 2010
    ..This reorientation has a layered structure that extends 2-3 nm from the cholesterol molecule. This study demonstrates that the Umbrella hypothesis is valid in both saturated and unsaturated lipid bilayers...
  9. pmc Conformations of islet amyloid polypeptide monomers in a membrane environment: implications for fibril formation
    Mojie Duan
    Gustaf H Carlson School of Chemistry and Biochemistry, Clark University, Worcester, Massachusetts, United States of America
    PLoS ONE 7:e47150. 2012
    ..This motif that bends at His18 resembles the overall topology of IAPP fibrils. The conformational preorganization into the fibril-like topology provides a possible explanation for the fast aggregation rate of S20G IAPP...
  10. ncbi Polar head groups are important for barrier-protective effects of oxidized phospholipids on pulmonary endothelium
    Anna A Birukova
    Section of Pulmonary and Critical Medicine, Department of Medicine, Division of Biomedical Sciences, University of Chicago, 929 East 57th St, CIS Bldg, W410, Chicago, IL 60637, USA
    Am J Physiol Lung Cell Mol Physiol 292:L924-35. 2007
    ....
  11. ncbi A method for separation of phosphatidylcholine, triacylglycerol, non-esterified fatty acids and cholesterol esters from plasma by solid-phase extraction
    G C Burdge
    Institute of Human Nutrition, University of Southampton, Southampton, UK
    Br J Nutr 84:781-7. 2000
    ..There was no evidence of selective elution of individual fatty acid or CE species. In conclusion, this method represents an efficient, rapid alternative to TLC for isolation of these lipid classes from plasma...
  12. ncbi Lecithinized superoxide dismutase improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats
    Tamiji Tsubokawa
    Department of Physiology, Loma Linda University Medical Center, Loma Linda, CA 92354, USA
    Stroke 38:1057-62. 2007
    ..We tested the effects of PC-SOD in focal cerebral ischemia in the rat middle cerebral artery occlusion model (MCAO)...
  13. pmc Mechanism of islet amyloid polypeptide fibrillation at lipid interfaces studied by infrared reflection absorption spectroscopy
    D H J Lopes
    University of Dortmund, Department of Chemistry, Physical Chemistry I Biophysical Chemistry, D 44227 Dortmund, Germany
    Biophys J 93:3132-41. 2007
    ..We may assume that these dynamical conformational changes observed for negatively charged lipid interfaces play an important role as the first steps of IAPP-induced membrane damage in type II diabetes...
  14. pmc Structure of lipid bilayers
    J F Nagle
    Department of Physics, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1469:159-95. 2000
    ..The uncertainty in structural results for lipid bilayers is being reduced and best current values are provided for bilayers of five lipids...
  15. ncbi Physiological regulation of phospholipid methylation alters plasma homocysteine in mice
    Rene L Jacobs
    Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids and Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 280:28299-305. 2005
    ..These data suggest that phospholipid methylation in the liver is a major consumer of AdoMet and a significant source of plasma Hcy...
  16. ncbi Visualization of phosphatidylcholine, lysophosphatidylcholine and sphingomyelin in mouse tongue body by matrix-assisted laser desorption/ionization imaging mass spectrometry
    Hirofumi Enomoto
    Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1 20 1 Handayama, Higashi ku, Hamamatsu, Shizuoka, 431 3192, Japan
    Anal Bioanal Chem 400:1913-21. 2011
    ..This study represents the first visualization of PC, LPC and SM localization in the mouse tongue body...
  17. ncbi Peritoneal retention of liposomes: Effects of lipid composition, PEG coating and liposome charge
    S Dadashzadeh
    Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
    J Control Release 148:177-86. 2010
    ..DOTAP/PEG100 may also be a more efficient formulation because this formulation can provide a high level of anticancer drug into the peritoneal cavity and also can passively target the primary tumor...
  18. pmc Lipid interaction and membrane perturbation of human islet amyloid polypeptide monomer and dimer by molecular dynamics simulations
    Yun Zhang
    State Key Laboratory of Surface Physics, and Department of Physics, Fudan University, Shanghai, China
    PLoS ONE 7:e38191. 2012
    ..This study provides insight into the hIAPP-membrane interaction and the molecular mechanism of membrane disruption by hIAPP oligomers...
  19. pmc Microsecond simulations indicate that ethanol binds between subunits and could stabilize an open-state model of a glycine receptor
    Samuel Murail
    Department of Theoretical Physics, Royal Institute of Technology, Stockholm, Sweden
    Biophys J 100:1642-50. 2011
    ..Finally, ethanol appears to stabilize the GlyR model built on a presumably open form of the ligand-gated channel. This stabilization could help explain the effects of allosteric ligand binding in Cys-loop receptors...
  20. ncbi Biosynthesis of phosphatidylcholine in bacteria
    Christian Sohlenkamp
    Centro de Investigacion Sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Apdo Postal 565 A, Cuernavaca, Morelos, Mexico
    Prog Lipid Res 42:115-62. 2003
    ..The occurrence of two intermediates in prokaryotes usually found as intermediates in the eukaryotic CDP-choline pathway for PC biosynthesis raises the question whether some bacteria might form PC via a CDP-choline pathway...
  21. ncbi Nrf2 regulates antioxidant gene expression evoked by oxidized phospholipids in endothelial cells and murine arteries in vivo
    Henna Kaisa Jyrkkänen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute, University of Kuopio, Finland
    Circ Res 103:e1-9. 2008
    ..We suggest that the activation of Nrf2 by oxidized phospholipids provides a mechanism by which their deleterious effects are limited in the vasculature...
  22. ncbi Function and pathophysiological importance of ABCB4 (MDR3 P-glycoprotein)
    Ronald P J Oude Elferink
    AMC Liver Center, Academic Medical Center, Amsterdam, The Netherlands
    Pflugers Arch 453:601-10. 2007
    ..In this review, we will discuss the functional aspects of ABCB4 and the regulation of its expression. Furthermore, we will describe the clinical and biochemical consequences of complete and partial deficiency of ABCB4 function...
  23. pmc Overexpression of the Arabidopsis 10-kilodalton acyl-coenzyme A-binding protein ACBP6 enhances freezing tolerance
    Qin Fang Chen
    School of Biological Sciences, University of Hong Kong, Pokfulam, Hong Kong, China
    Plant Physiol 148:304-15. 2008
    ....
  24. ncbi Plasma sphingomyelin level as a risk factor for coronary artery disease
    X C Jiang
    Department of Medicine, Columbia University, New York, NY 10032, USA
    Arterioscler Thromb Vasc Biol 20:2614-8. 2000
    ..Plasma SM levels could be a marker for atherogenic remnant lipoprotein accumulation and may predict lipoprotein susceptibility to arterial wall sphingomyelinase...
  25. ncbi Interaction of the antimicrobial peptide pheromone Plantaricin A with model membranes: implications for a novel mechanism of action
    Hongxia Zhao
    Helsinki Biophysics and Biomembrane Group, Institute of Biomedicine, University of Helsinki, Finland
    Biochim Biophys Acta 1758:1461-74. 2006
    ....
  26. pmc Cholesterol-induced modifications in lipid bilayers: a simulation study
    S W Chiu
    Department of Molecular and Integrative Physiology, UIUC Programs in Biophysics, Neuroscience, and Bioengineering, and Beckman Institute, University of Illinois, Urbana 61801, USA
    Biophys J 83:1842-53. 2002
    ..We are continuing to analyze this tendency on longer timescales and for larger bilayer patches...
  27. pmc Characterization of cholesterol-sphingomyelin domains and their dynamics in bilayer membranes
    A V Samsonov
    Rush Medical College, Department of Molecular Biophysics and Physiology, Chicago, Illinois 60612, USA
    Biophys J 81:1486-500. 2001
    ..The fluid nature of rafts, demonstrated in this study, may be important for permitting dynamic interactions between proteins localized within rafts...
  28. pmc Impaired de novo choline synthesis explains why phosphatidylethanolamine N-methyltransferase-deficient mice are protected from diet-induced obesity
    Rene L Jacobs
    Group on the Molecular and Cell Biology of Lipids and Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 285:22403-13. 2010
    ..Thus, de novo synthesis of choline via PEMT has a previously unappreciated role in regulating whole body energy metabolism...
  29. ncbi A class of potent antimalarials and their specific accumulation in infected erythrocytes
    Kai Wengelnik
    CNRS UMR 5539, CP 107, CNRS UMR 5810, CP 22, Universite Montpellier II, Place E Bataillon, 34095 Montpellier Cedex 5, France
    Science 295:1311-4. 2002
    ..falciparum and P. cynomolgi...
  30. pmc Mass spectrometric imaging of highly curved membranes during Tetrahymena mating
    Sara G Ostrowski
    Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA
    Science 305:71-3. 2004
    ..This evidence suggests that biological fusion involves and might in fact be driven by a heterogeneous redistribution of lipids at the fusion site...
  31. ncbi Effect of recombinant ApoA-I Milano on coronary atherosclerosis in patients with acute coronary syndromes: a randomized controlled trial
    Steven E Nissen
    Department of Cardiovascular Medicine, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    JAMA 290:2292-300. 2003
    ..Infusion of recombinant ApoA-I Milano-phospholipid complexes produces rapid regression of atherosclerosis in animal models...
  32. pmc Recent progress on acyl CoA: lysophospholipid acyltransferase research
    Hideo Shindou
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Lipid Res 50:S46-51. 2009
    ..These discoveries have prompted a robust surge of research in this field. In this review, we focus on the cloning and characterization of lysophospholipid acyltransferases (LPLATs), which contribute to membrane asymmetry and diversity...
  33. ncbi Modulation of membrane curvature by phosphatidic acid and lysophosphatidic acid
    Edgar E Kooijman
    Department of Biochemistry of Membranes, Center for Biomembranes and Lipid Enzymology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Traffic 4:162-74. 2003
    ..Alternative models for the regulation of membrane fission based on the strong dependence of the molecular shape of (lyso)phosphatidic acid on pH and divalent cations are also discussed...
  34. ncbi Oxidized phosphatidylcholines in membrane-level cellular signaling: from biophysics to physiology and molecular pathology
    Roman Volinsky
    Helsinki Biophysics and Biomembrane Group, Department of Biomedical Engineering and Computational Science, Aalto University, Espoo, Finland
    FEBS J 280:2806-16. 2013
    ....
  35. ncbi Kinetic analyses of liver phosphatidylcholine and phosphatidylethanolamine biosynthesis using (13)C NMR spectroscopy
    Nicholas V Reo
    Department of Biochemistry and Molecular Biology, WSU Magnetic Resonance Laboratory, Wright State University, Cox Institute, Dayton, OH 45429, USA
    Biochim Biophys Acta 1580:171-88. 2002
    ..04 micromol/h/g liver) was 2-3 times greater than that of PtdC de novo synthesis. The metabolic pathway information provided by these studies makes the NMR method superior to earlier radioisotope studies...
  36. ncbi The use of phosphatidylcholine for correction of lower lid bulging due to prominent fat pads
    P G Rittes
    Clínica Patrícia Rittes, Sao Paulo, Brazil
    Dermatol Surg 27:391-2. 2001
    ..Patients with prominent periorbital fat pads often complain of having a persistent "tired" look and seek treatment from their dermatologist...
  37. ncbi Randomized double-blind clinical trial of subcutaneously injected deoxycholate versus a phosphatidylcholine-deoxycholate combination for the reduction of submental fat
    Adam M Rotunda
    David Geffen School of Medicine, University of California at Los Angeles, 90095, USA
    Dermatol Surg 35:792-803. 2009
    ..Subcutaneous injections consisting of the detergent, sodium deoxycholate, alone or in combination with the phospholipid, phosphatidylcholine, have been reported to reduce adipose tissue. The role of phosphatidylcholine remains unclear...
  38. ncbi Membrane properties induced by anionic phospholipids and phosphatidylethanolamine are critical for the membrane binding and catalytic activity of human cytochrome P450 3A4
    Keon Hee Kim
    Department of Genetic Engineering, Paichai University, Taejon 302 735, Korea
    Biochemistry 42:15377-87. 2003
    ..Taken together, it can be suggested that the chemical and physical properties of membranes induced by anionic phospholipids and PE are critical for the membrane binding and catalytic activity of CYP3A4...
  39. pmc A lipid-mediated quality control process in the Golgi apparatus in yeast
    Ludovic Pineau
    Universite de Poitiers, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6161 Transport des assimilats 86022 Poitiers Cedex, France
    Mol Biol Cell 19:807-21. 2008
    ....
  40. pmc Hydration of POPC bilayers studied by 1H-PFG-MAS-NOESY and neutron diffraction
    Klaus Gawrisch
    Laboratory of Membrane Biochemistry and Biophysics, NIAAA, NIH, Bethesda, MD 20892, USA
    Eur Biophys J 36:281-91. 2007
    ..Water molecules penetrate deep into the interfacial region of bilayers but water concentration in the hydrophobic core is below the detection limit of one water molecule per lipid, in excellent agreement with the cross-relaxation data...
  41. pmc Molecular dynamics studies of transportan 10 (tp10) interacting with a POPC lipid bilayer
    Christina M Dunkin
    Department of Chemistry and Biochemistry, University of North Carolina, Wilmington, North Carolina 28403, USA
    J Phys Chem B 115:1188-98. 2011
    ..The electrostatic attraction between Lys and phosphate groups is also believed to be the main bottleneck for the translocation of tp10 across the membrane...
  42. pmc Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: boundaries and composition of lipid rafts
    Rodrigo F M de Almeida
    Centro de Quimica Fisica Molecular, Instituto Superior Tecnico, Universidade Tecnica de Lisboa, Lisbon, Portugal
    Biophys J 85:2406-16. 2003
    ..The diagrams here described are used to rationalize literature results, some of them apparently discrepant, and to discuss lipid rafts within the framework of liquid-ordered/liquid-disordered phase coexistence...
  43. ncbi Phosphatidylcholine biosynthesis and its significance in bacteria interacting with eukaryotic cells
    Meriyem Aktas
    Ruhr Universitat Bochum, Lehrstuhl für Biologie der Mikroorganismen, Universitatsstrasse 150, NDEF 06 783, 44780 Bochum, Germany
    Eur J Cell Biol 89:888-94. 2010
    ..In the Pcs pathway, choline is condensed directly with CDP-diacylglycerol to form PC. This review focuses on the biosynthetic pathways and the significance of PC in bacteria with an emphasis on plant-microbe interactions...
  44. ncbi Solubilization of poorly water-soluble drugs by mixed micelles based on hydrogenated phosphatidylcholine
    Christopher Rupp
    Department of Pharmaceutics and Biopharmaceutics, Christian Albrecht University Kiel, Gutenbergstrasse 76, 24118 Kiel, Germany
    Int J Pharm 395:272-80. 2010
    ..Constant CMC-values could be obtained regardless if diazepam was encapsulated within the MM or unloaded MM were analysed...
  45. ncbi Phosphatidylcholine in the treatment of localized fat
    Doris Hexsel
    Doris Hexsel Dermatologic Clinic, Porto Alegre, Brazil
    J Drugs Dermatol 2:511-8. 2003
    ..From the authors' point of view, the off-label use of phosphatidylcholine in the treatment of fatty pads and small areas of localized fat is safe, low cost, and effective...
  46. ncbi Ganglioside G(M1)-mediated amyloid-beta fibrillogenesis and membrane disruption
    Eva Y Chi
    Department of Chemistry, The Institute for Biophysical Dynamics, and The James Franck Institute, University of Chicago, 929 E 57th Street, Chicago, Illinois 60637, USA
    Biochemistry 46:1913-24. 2007
    ....
  47. ncbi Plant-exuded choline is used for rhizobial membrane lipid biosynthesis by phosphatidylcholine synthase
    K E de Rudder
    Institute of Biotechnology, Technical University of Berlin, Seestrasse 13, D 13353 Berlin, Germany
    J Biol Chem 274:20011-6. 1999
    ..meliloti. We further demonstrate that roots of host plants of S. meliloti exude choline and that the amounts of exuded choline are sufficient to allow for maximal phosphatidylcholine biosynthesis in S. meliloti via the novel pathway...
  48. ncbi Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortus
    Raquel Conde-Alvarez
    Departamento de Microbiologia, Universidad de Navarra, Pamplona, Spain
    Cell Microbiol 8:1322-35. 2006
    ..The results suggest that Pcs and PmtA have complementary roles in vivo related to nutrient availability and that PC and the membrane properties that depend on this typical eukaryotic phospholipid are essential for Brucella virulence...
  49. ncbi Massively parallel dip-pen nanolithography of heterogeneous supported phospholipid multilayer patterns
    Steven Lenhert
    Institut fur Nanotechnologie, Forschungszentrum Karlsruhe GmbH, 76021 Karlsruhe, Germany
    Small 3:71-5. 2007
  50. pmc Composition and localization of lipids in Penaeus merguiensis ovaries during the ovarian maturation cycle as revealed by imaging mass spectrometry
    Piyachat Chansela
    Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
    PLoS ONE 7:e33154. 2012
    ..In this study, we report on the compositions of major lipids, i.e. phosphatidylcholines (PCs), triacylglycerols (TAGs), and fatty acids (FAs), in the ovaries of the banana shrimp, Penaeus ..
  51. ncbi Role of the yeast phosphatidylinositol/phosphatidylcholine transfer protein (Sec14p) in phosphatidylcholine turnover and INO1 regulation
    J L Patton-Vogt
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 2683, USA
    J Biol Chem 272:20873-83. 1997
    ....
  52. pmc Perturbations of membrane structure by cholesterol and cholesterol derivatives are determined by sterol orientation
    Brett N Olsen
    Molecular Cell Biology Graduate Program, Center for Computational Biology, Washington University in St Louis, 700 South Euclid Avenue, Campus Box 8036, St Louis, Missouri 63110, USA
    J Am Chem Soc 131:4854-65. 2009
    ..This study shows that direct membrane perturbation by side-chain oxysterols is significant and suggests that these membrane perturbations may play a role in the oxysterol regulation of cholesterol homeostasis...
  53. ncbi Molecular dynamics simulations of T-20 HIV fusion inhibitor interacting with model membranes
    A M T Martins do Canto
    Departamento de Quimica, Universidade de Evora, Portugal
    Biophys Chem 159:275-86. 2011
    ..This lower ability to interact with membranes is probably correlated with its smaller inhibitory efficiency...
  54. ncbi Development of imaging mass spectrometry (IMS) dataset extractor software, IMS convolution
    Takahiro Hayasaka
    Department of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, Higashi ku, Hamamatsu, Shizuoka, Japan
    Anal Bioanal Chem 401:183-93. 2011
    ..Therefore, we believe that IMS Convolution with ROIs could automatically extract the meaningful peaks from large-volume IMS datasets for inexperienced users as well as for researchers who have performed the analysis...
  55. ncbi In vitro evaluation of liposomes containing bio-enhancers for the oral delivery of macromolecules
    Johannes Parmentier
    Department of Pharmaceutical Technology and Biopharmacy, University of Heidelberg, Heidelberg, Germany
    Eur J Pharm Biopharm 76:394-403. 2010
    ..5-fold without showing any cell toxicity or decrease in TER should be safe and effective candidates for the development of an oral delivery system for hydrophilic macromolecules...
  56. pmc 2H nuclear magnetic resonance order parameter profiles suggest a change of molecular shape for phosphatidylcholines containing a polyunsaturated acyl chain
    L L Holte
    Section of NMR Studies, NIAAA, National Institutes of Health, Rockville, Maryland 20852, USA
    Biophys J 68:2396-403. 1995
    ..resonance spectroscopy was used to determine the orientational order parameter profiles for a series of phosphatidylcholines with perdeuterated stearic acid, 18:0d35, in position sn-1 and 18:1 omega 9, 18:2 omega 6, 18:3 omega 3, 20:..
  57. ncbi Differential effect of surfactant and its saturated phosphatidylcholines on human blood macrophages
    Christian Gille
    University Children s Hospital, Department of Neonatology, Calwerstr 7, 72076 Tuebingen, Germany
    J Lipid Res 48:307-17. 2007
    ..These data identify individual surfactant PC species as modifiers of macrophage differentiation and suggest differential effects on innate and adaptive immune functions...
  58. ncbi Impedance analysis of phosphatidylcholine membranes modified with valinomycin
    Monika Naumowicz
    Institute of Chemistry, University of Bialystok, Al J Pilsudskiego 11 4, 15 443 Bialystok, Poland
    Eur Biophys J 35:239-46. 2006
    ....
  59. pmc Lipid lateral diffusion in ordered and disordered phases in raft mixtures
    Andrey Filippov
    Department of Biophysical Chemistry, Umea University, SE 901 87 Umea, Sweden
    Biophys J 86:891-6. 2004
    ..This means that on the millisecond timescale fluid, ordered domains are floating around in a sea of faster diffusing lipids, assigned to consist of mainly dioleoylphosphatidylcholine...
  60. ncbi Binding of antibiotic amphotericin B to lipid membranes: a 1H NMR study
    Janina Gabrielska
    Department of Physics and Biophysics, Agricultural University, Wrocław, Poland
    FEBS Lett 580:2677-85. 2006
    ..The same effects are also observed in the sterol-containing membranes, except that the effect on the hydrophobic core was exclusively observed in the membranes containing ergosterol...
  61. pmc Sphingomyelin-cholesterol domains in phospholipid membranes: atomistic simulation
    Sagar A Pandit
    Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, Illinois 60616, USA
    Biophys J 87:1092-100. 2004
    ..The calculated difference in thickness is consistent with data obtained in atomic force microscopy experiments...
  62. ncbi Mesotherapy and phosphatidylcholine injections: historical clarification and review
    Adam M Rotunda
    Department of Dermatology, University of Southern California School of Medicine, Los Angeles, California, USA
    Dermatol Surg 32:465-80. 2006
    ..Despite their attraction as purported "fat-dissolving" injections, the safety and efficacy of these novel cosmetic treatments remain ambiguous to most patients and physicians...
  63. ncbi Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane
    Mirja Wessel
    Lehrstuhl für Biologie der Mikroorganismen, Ruhr Universitat Bochum, Bochum, Germany
    Mol Microbiol 62:906-15. 2006
    ..Our results strongly suggest that PC in bacterial membranes is an important determinant for the establishment of host-microbe interactions...
  64. ncbi Use of phosphatidylcholine for the correction of lower lid bulging due to prominent fat pads
    Patrick J Treacy
    Department of Skin Research, Ailesbury Clinic, Dublin, Ireland
    J Cosmet Laser Ther 8:129-32. 2006
    ..However, there is a dearth of clinical data relating to efficacy and side effects...
  65. ncbi Prolongation of residence time of liposome by surface-modification with mixture of hydrophilic polymers
    Tamer Shehata
    Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University, 1 1 1 Tsushima naka, Okayama 700 8530, Japan
    Int J Pharm 359:272-9. 2008
    ..The decrease in the adsorbed amount of several opsonins and the increase in the adsorbed dysopsonins would be responsible for its lower affinity to the liver and long residence in the systemic circulation of PEG4%/PVA1% liposome...
  66. pmc Cholesterol packing around lipids with saturated and unsaturated chains: a simulation study
    Sagar A Pandit
    Department of Physics, University of South Florida, Tampa, FL 33620, USA
    Langmuir 24:6858-65. 2008
    ..Physically, this appears to be related to the fact that the two faces of the cholesterol molecule are different from each other and that the steric cross section of cholesterol molecules drops sharply near the small chain tails...
  67. pmc Loss of lysophosphatidylcholine acyltransferase 1 leads to photoreceptor degeneration in rd11 mice
    James S Friedman
    Neurobiology Neurodegeneration and Repair Laboratory, Biological Imaging Core, and Mechanisms of Retinal Diseases Section, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:15523-8. 2010
    ..Our studies add another dimension to an essential role for LPCAT1 in retinal photoreceptor homeostasis...
  68. ncbi Oxidized phospholipids are more potent antagonists of lipopolysaccharide than inducers of inflammation
    Olga V Oskolkova
    Department of Vascular Biology and Thrombosis Research, Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstrasse 17, A 1090 Vienna, Austria
    J Immunol 185:7706-12. 2010
    ..Quantification of the most abundant oxidized phosphatidylcholines by HPLC/tandem mass spectrometry showed that circulating concentrations of total oxidized ..
  69. pmc Mixed micelles loaded with silybin-polyene phosphatidylcholine complex improve drug solubility
    Rui Ling Duan
    Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
    Acta Pharmacol Sin 32:108-15. 2011
    ..To prepare a novel formulation of phosphatidylcholine (PC)-bile salts (BS)-mixed micelles (MMs) loaded with silybin (SLB)-PC complex for parenteral applications...
  70. ncbi Analysis of short-term changes in the Arabidopsis thaliana glycerolipidome in response to temperature and light
    Asdrubal Burgos
    Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam Golm, Germany
    Plant J 66:656-68. 2011
    ..Observed changes are discussed in the context of current knowledge, and new hypotheses have been formulated concerning the early stages of the plant response to changing light and temperature conditions...
  71. pmc 2H-NMR study and molecular dynamics simulation of the location, alignment, and mobility of pyrene in POPC bilayers
    Barbara Hoff
    IMVM, Bioprocess Engineering, University of Karlsruhe, 76131 Karlsruhe, Germany
    Biophys J 88:1818-27. 2005
    ..The good agreement can be explained by the fact that the different kind of motions of a pyrene molecule are already averaged within a few nanoseconds, which is the timescale covered by the MD simulation...
  72. pmc A gating model for the archeal voltage-dependent K(+) channel KvAP in DPhPC and POPE:POPG decane lipid bilayers
    Daniel Schmidt
    Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
    J Mol Biol 390:902-12. 2009
    ..By delaying the voltage sensor's return to the hyperpolarized conformation, VSTx1 favors the inactivated state of KvAP...
  73. pmc Activation of aortic endothelial cells by oxidized phospholipids: a phosphoproteomic analysis
    Alejandro Zimman
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California 90095 1770, USA
    J Proteome Res 9:2812-24. 2010
    ..Many changes in protein phosphorylation induced by Ox-PAPC are reported here for the first time and provide new insights into the mechanism of activation by oxidized lipids, including phosphorylation-based signal transduction...
  74. pmc Dynamic structure of vesicle-bound melittin in a variety of lipid chain lengths by solid-state NMR
    Shuichi Toraya
    Faculty of Engineering, Yokohama National University, Yokohama, Japan
    Biophys J 87:3323-35. 2004
    ..These analyses further lead to the conclusion that the alpha-helices of melittin molecules penetrate the hydrophobic cores of the bilayers incompletely as a pseudo-trans-membrane structure and induce fusion and disruption of vesicles...
  75. ncbi Multiphoton excitation fluorescence microscopy in planar membrane systems
    Jonathan Brewer
    Membrane Biophysics and Biophotonics group MEMPHYS, Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
    Biochim Biophys Acta 1798:1301-8. 2010
    ..This correspondence occurs when the lateral pressure of the monolayer is 26+/-2 mN/m and 28+/-3 mN/m for DOPC and DPPC, respectively...
  76. ncbi Increase of oxidant-related triglycerides and phosphatidylcholines in serum and small intestinal mucosa during development of intestinal polyp formation in Min mice
    Kazutaka Ikeda
    Department of Metabolome, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Cancer Sci 102:79-87. 2011
    ..As a result, oxidized phosphatidylcholines (PC) such as aldehyde and carboxylic acid types were increased, even at an early stage of intestinal polyp ..
  77. ncbi Phospholipids block nuclear factor-kappa B and tau phosphorylation and inhibit amyloid-beta secretion in human neuroblastoma cells
    N R Pandey
    Lipoprotein and Atherosclerosis Research Group, University of Ottawa Heart Institute, 40 Ruskin Street, Ottawa, ON K1Y 4W7, Canada
    Neuroscience 164:1744-53. 2009
    ..Taken together, these data suggest that DLPC can act through MAPK to block neuronal inflammatory cascades and prevent potential pathological consequences in the neuronal metabolism of amyloid and tau proteins...
  78. ncbi Oxidized phospholipids mediate occludin expression and phosphorylation in vascular endothelial cells
    Lucas DeMaio
    Dept of Biomedical Engineering and Division of Cardiovascular Medicine, DRB 398, USC, Los Angeles, CA 90089, USA
    Am J Physiol Heart Circ Physiol 290:H674-83. 2006
    ..OxPAPC-induced disruption of TJs, which likely facilitates transmigration of LDL and inflammatory cells into the subendothelial layers, may be mediated by reactive oxygen species...
  79. ncbi Molecular dynamics simulations of unsaturated lipid bilayers: effects of varying the numbers of double bonds
    Marja T Hyvönen
    Wihuri Research Institute, Kalliolinnantie 4, 00140 Helsinki, Finland
    Eur Biophys J 34:294-305. 2005
    ..Moreover, the double bond region of tetraunsaturated chains is shown to span all the way from the bilayer centre to the head group region...
  80. pmc Unfolding and extraction of a transmembrane alpha-helical peptide: dynamic force spectroscopy and molecular dynamics simulations
    Sonia Antoranz Contera
    Bionanotechnology IRC, Physics Department, University of Oxford, Oxford OX1 3PU, United Kingdom
    Biophys J 89:3129-40. 2005
    ..The second takes into account the ramped bias that the linker feels as it is being driven out of the adhesion complex and scales as F approximately (ln V)2/3...
  81. ncbi Measurement of pulmonary surfactant disaturated-phosphatidylcholine synthesis in human infants using deuterium incorporation from body water
    Paola E Cogo
    Department of Pediatrics, University of Padova, Padova, Italy
    J Mass Spectrom 40:876-81. 2005
    ..This study is a simpler and less invasive method compared to previously published methods on surfactant kinetics by means of stable isotopes...
  82. ncbi Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes
    Paola Cogo
    Department of Pediatrics, University of Padova, Padova, and Pharmacy, Salesi Children Hospital, Ancona, Italy
    Pediatr Res 57:519-22. 2005
    ..SP-B half-life seemed to be shorter than DSPC half-life. These results agree with existing animal data. We conclude that the measurement of SP-B kinetics is feasible in vivo in humans by stable isotope technology...
  83. pmc Liposomes, disks, and spherical micelles: aggregate structure in mixtures of gel phase phosphatidylcholines and poly(ethylene glycol)-phospholipids
    Markus Johnsson
    Department of Physical Chemistry, Uppsala University, SE 751 23 Uppsala, Sweden
    Biophys J 85:3839-47. 2003
    ..Increasing the temperature, from 25 degrees C to above the gel-to-liquid crystalline phase transition temperature for the respective lipid mixtures, has a relatively small effect on the aggregate structure...
  84. ncbi Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3
    Toshiaki Houjou
    Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya City, Aichi 467 8603, Japan
    Rapid Commun Mass Spectrom 18:3123-30. 2004
    ..The present paper deals only with qualitative identification of individual molecular species, and the related quantitative studies are now underway...
  85. ncbi Voltage gating of VDAC is regulated by nonlamellar lipids of mitochondrial membranes
    Tatiana K Rostovtseva
    Laboratory of Physical and Structural Biology and National Center for Medical Rehabilitation Research, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:37496-506. 2006
    ..We propose that the specific lipid composition of the mitochondria outer membrane and/or of contact sites might influence MOM permeability by regulating VDAC gating...
  86. ncbi Investigation of natural phosphatidylcholine sources: separation and identification by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2) of molecular species
    Julie Le Grandois
    Laboratoire de Chimie Analytique et Sciences de l Aliment, IPHC DSA, Universite de Strasbourg, CNRS, 67400 Illkirch, France
    J Agric Food Chem 57:6014-20. 2009
    ..b>Phosphatidylcholines (PCs) were purified from total lipid extracts of different food matrices, and their molecular species were ..
  87. ncbi Acid-base equilibria at interface separating electrolyte solution and lipid bilayer formed from phosphatidylcholine
    Aneta D Petelska
    Laboratory of Interfacial Electrochemistry, Faculty of Chemistry, University of Warsaw, Pasteur St 1, 02 093 Warsaw, Poland
    Biophys Chem 104:13-9. 2003
    ..We take suitable equations to describe the dependence of interfacial tension of a lipid bilayer membrane on H(+) and OH(-) ion concentrations. A theoretical equation is derived to describe this dependence in the whole pH range...
  88. ncbi The interfacial tension of the lipid membrane formed from lipid-cholesterol and lipid-lipid systems
    Aneta D Petelska
    Institute of Chemistry, University of Bialystok, Al J Pilsudskiego 11 4, 15 443 Bialystok, Poland
    Cell Biochem Biophys 44:205-11. 2006
    ....
  89. ncbi Polyunsaturation in lipid membranes: dynamic properties and lateral pressure profiles
    Samuli Ollila
    Laboratory of Physics and Helsinki Institute of Physics, Helsinki University of Technology, P O Box 1100, FI 02015 HUT, Finland
    J Phys Chem B 111:3139-50. 2007
    ..This is compensated for by changes in the membrane-water interface region in terms of increasing peak heights of the lateral pressure profile. Implications of the findings are briefly discussed...
  90. ncbi Alterations of phospholipid concentration and species composition of the intestinal mucus barrier in ulcerative colitis: a clue to pathogenesis
    Annika Braun
    Department of Gastroenterology, University Hospital Heidelberg, Heidelberg, Germany
    Inflamm Bowel Dis 15:1705-20. 2009
    ..The objective of this study was to systematically investigate phospholipids in the intestinal mucus of humans suffering from inflammatory bowel diseases, where a barrier defect is strongly supposed to be pathogenetic...
  91. pmc XBP1: a link between the unfolded protein response, lipid biosynthesis, and biogenesis of the endoplasmic reticulum
    Rungtawan Sriburi
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
    J Cell Biol 167:35-41. 2004
    ..These data suggest that XBP1(S) links the mammalian UPR to phospholipid biosynthesis and ER biogenesis...
  92. pmc High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells
    Dylan M Owen
    Chemical Biology Centre, Imperial College, London, UK
    Mol Membr Biol 27:178-89. 2010
    ..The observed spatial patterning of membrane order in the immunological synapse depends on active receptor signalling...
  93. pmc p190RhoGAP mediates protective effects of oxidized phospholipids in the models of ventilator-induced lung injury
    Anna A Birukova
    Lung Injury Center, Section of Pulmonary and Critical Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, USA
    Exp Cell Res 317:859-72. 2011
    ..These results demonstrate for the first time a key role of p190RhoGAP for the vascular endothelial barrier protection in VILI...
  94. ncbi A choline-deficient diet exacerbates fatty liver but attenuates insulin resistance and glucose intolerance in mice fed a high-fat diet
    Peter J Raubenheimer
    University of Edinburgh, Centre for Cardiovascular Science, Queen s Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK
    Diabetes 55:2015-20. 2006
    ..We conclude that liver fat accumulation per se does not cause insulin resistance during high-fat feeding and that choline deficiency may shunt potentially toxic free fatty acids toward innocuous storage triglyceride in the liver...
  95. ncbi Inhibition of LPS- and CpG DNA-induced TNF-alpha response by oxidized phospholipids
    Zheng Ma
    Center for Pharmacogenetics, Univ of Pittsburgh School of Pharmacy, 639 Salk Hall, Pittsburgh, PA 15213, USA
    Am J Physiol Lung Cell Mol Physiol 286:L808-16. 2004
    ..Further studies on the mechanisms of action may lead to development of a new type of anti-inflammatory drug for treatment of acute inflammatory diseases such as sepsis...
  96. ncbi Paxillin-beta-catenin interactions are involved in Rac/Cdc42-mediated endothelial barrier-protective response to oxidized phospholipids
    Anna A Birukova
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Am J Physiol Lung Cell Mol Physiol 293:L199-211. 2007
    ..In turn, paxillin knockdown by specific siRNA attenuated AJ enhancement in response to OxPAPC. These results show for the first time the novel interactions between FA and AJ protein complexes critical for EC barrier regulation by OxPAPC...
  97. ncbi Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine
    Mohammad Reza Hojjati
    Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
    J Lipid Res 47:673-6. 2006
    ..6 +/- 3.8% for PC. The interassay coefficient of variation of the assay was 1.7 +/- 0.05% for SM and 3.1 +/- 0.13% for PC. These two new methods are amenable to automation and can be adapted for large-scale, high-throughput assays...
  98. pmc Microscopic observations reveal that fusogenic peptides induce liposome shrinkage prior to membrane fusion
    Fumimasa Nomura
    Department of Molecular Biology, School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 101:3420-5. 2004
    ..Our results suggest that a perturbation of lipid bilayers, which probably resulted from alterations in the bending folds of membranes, is a critical factor in fusion efficiency...
  99. pmc A correlation between lipid domain shape and binary phospholipid mixture composition in free standing bilayers: A two-photon fluorescence microscopy study
    L A Bagatolli
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
    Biophys J 79:434-47. 2000
    ..This last finding extends previous observations of GUVs composed of DPPE/DPPC and DLPC/DPPC mixtures (, Biophys. J. 78:290-305)...
  100. pmc Amphotericin B channels in the bacterial membrane: role of sterol and temperature
    Berenice Venegas
    Centro de Ciencias Fisicas, UNAM, 62251 Cuernavaca, Morelos, Mexico
    Biophys J 85:2323-32. 2003
    ..The results support the idea that membrane structure is a determining factor in the action of the antibiotic...
  101. ncbi An unexpected requirement for phosphatidylethanolamine N-methyltransferase in the secretion of very low density lipoproteins
    Anna A Noga
    Department of Biochemistry and the Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Canada
    J Biol Chem 277:42358-65. 2002
    ..Because the levels of PC in the hepatocytes and hepatoma cells were unaffected by the lack of PEMT expression, there appears to be an unexpected requirement for PEMT in the secretion of apoB100-containing VLDLs...

Research Grants72

  1. Prostate MRI and MRS: Correlations with Gene Expression
    Sandra Gaston; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  2. Retinoid Metabolism and Alcohol Induced Disease
    William S Blaner; Fiscal Year: 2010
    ..We are proposing studies that will provide a more complete understanding of relationships between alcohol consumption, vitamin A metabolism and actions, and alcoholic organ damage. ..
  3. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Alex Brown; Fiscal Year: 2010
    ..The funds requested in this proposal will make an invaluable contribution to the support of this conference and in particular the development of young investigators. ..
  4. Deregulated lipid metabolism in stroke
    Robert J Dempsey; Fiscal Year: 2013
    ..This proposal explores the therapeutic potential of D609 and how it affects lipid second messenger ceramide that regulates the cell cycle both in vitro and in vivo stroke models. ..
  5. Regulation of Hepatic Lipid and Glucose Metabolism by PC-TP/StarD2
    David E Cohen; Fiscal Year: 2013
    ..signaling;2) To ascertain the pathogenic roles of PC-TP and Them2 in ER stress;and 3) To define roles of phosphatidylcholines in the activation of Them2 and TSC2 by PC-TP...
  6. Lysosomal phospholipase A2 in autoimmune disease
    JAMES ALAN SHAYMAN; Fiscal Year: 2013
    ..Understanding the mechanisms whereby impaired degradation of phospholipids result in the autoimmune phenotype may provide new insights into the pathogenesis of lupus and potential new targets for therapy. ..
  7. REGULATION OF PHOSPHOLIPID SYNTHESIS
    George M Carman; Fiscal Year: 2013
    ..The proposed work will shed light on how PAP activity is regulated, and open new avenues for understanding the control and integration of convergent and divergent lipid metabolic pathways emanating from PA. ..
  8. Phospholipid-Mediated Metabolic Control in Liver and Brown Adipose Tissue
    David E Cohen; Fiscal Year: 2013
    ..The central hypothesis is that key regulatory events occur when PC-TP binds specific membrane phosphatidylcholines and then activates thioesterase superfamily member 2 (Them2)...
  9. Lipoprotein Structure and Function by Individual Particle Electron Tomography
    Gang Ren; Fiscal Year: 2013
    ....
  10. GI-Safer Formulation of Indomethacin for use in Preterm Neonates
    UPENDRA K MARATHI; Fiscal Year: 2010
    ..This grant proposal will partially fund the development of a new molecular complex of indomethacin and phosphatidylcholine, a new formulation of indomethacin that significantly reduces GI injury. ..
  11. GI-Safer Formulation of Indomethacin for use in Preterm Neonates
    UPENDRA K MARATHI; Fiscal Year: 2013
    ....
  12. Control of liver autophagy by phosphatidic acid signaling
    GILBERT DIPAOLO; Fiscal Year: 2013
    ....
  13. Shared Mechanisms of Valvular and Vascular Calcification
    JOEL DAVID MORRISETT; Fiscal Year: 2012
    ....
  14. Treatment of Acute Coronary Syndromes with Recombinant LCAT Infusion
    BRIAN ROBERT KRAUSE; Fiscal Year: 2011
    ....
  15. Roles for Phospholipase D2 in metabolism and cardiovascular homeostasis
    ROCHELLE NELSON; Fiscal Year: 2013
    ..These studies will also provide information necessary for new pharmacological targets aimed at the prevention of cardiovascular disease risk factors including hypertension and atherosclerosis. ..
  16. Regulation of Hepatic Glucose Homeostasis by Phosphatidylcholine Transfer Protein
    Baran Ersoy; Fiscal Year: 2012
    ..regulates glucose metabolism, which is significant because the faty acyl composition of the membrane phosphatidylcholines varies in health and disease...
  17. Nanoliposome-based Treatment of Amyloid Protein (AL) Toxicity
    RAYMOND QUEZON MIGRINO; Fiscal Year: 2013
    ....
  18. Host-associated regulation of P. aeruginosa colonization and virulence
    Deborah A Hogan; Fiscal Year: 2013
    ..aeruginosa rendering it more susceptible to existing antimicrobial therapies. ..
  19. PHOSPHOLIPID METABOLISM AND MEMBRANE FUNCTION
    George M Carman; Fiscal Year: 2013
    ..We will examine the role and regulation of the DGK1-encoded DAG kinase enzyme in this metabolism (specific aim 3). ..
  20. Roles of Phospholipase D in AT1 Receptor Endocytosis
    Guangwei Du; Fiscal Year: 2010
    ..AT1R activation in different subcellular locations results in distinct signaling outputs. We will determine whether PLD2 downregulation changes the kinetic and spatial patterns of Ang II signaling, and as a result, affects cell growth. ..
  21. Inhibition of Fat Absorption as a Mechanism to Treat Obesity
    CHARLES MILTON MANSBACH; Fiscal Year: 2013
    ..These changes will also be correlated with PCTV generating activity of the ER. This will give physiological conformation of our in vitro findings. ..
  22. Gut flora metabolism of dietary phosphatidylcholine and cardiovascular disease
    Stanley L Hazen; Fiscal Year: 2013
    ....
  23. Roles of Sphingolipids in the Pathophysiology of Obesity and Diabetes
    LAUREN ASHLEY COWART; Fiscal Year: 2013
    ....
  24. Genetic regulation of racial differences in platelet reactivity
    MICHAEL ALLAN HOLINSTAT; Fiscal Year: 2013
    ..Understanding the racial difference in platelet activity will fill a significant gap in our understanding of why blacs suffer a higher morbidity and mortality than whites following MI and stroke. ..
  25. NSAID-Phosphatidylcholine Association: Insight in GI Ulcer Pathogenesis/Therapy
    Lenard M Lichtenberger; Fiscal Year: 2010
    ..Furthermore, by pre-associating soy PC with NSAIDs we have developed a novel family GI-safer, therapeutically effective PC-NSAIDs that can be made available to the public using an accelerated FDA regulatory pathway. ..
  26. Anionic Surfactant Lipid Regulation of Inflammation and Infection in the Lung
    Dennis R Voelker; Fiscal Year: 2013
    ....
  27. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani V Subbaiah; Fiscal Year: 2012
    ..These studies will provide novel insights into the physiological role of this important phospholipid, and could possibly identify novel therapeutic targets against inflammation and atherosclerosis. ..
  28. ALIMENTARY TRACT LIPIDS IN HEALTH AND DISEASE
    MARTIN CONRAD CAREY; Fiscal Year: 2013
    ..abstract_text> ..
  29. Myelin Composition Influence on Bilayer Interactions in Multiple Sclerosis
    Jacob N Israelachvili; Fiscal Year: 2010
    ....
  30. Synthetic surfactant and toxic chemical lung injury
    Frans J Walther; Fiscal Year: 2011
    ....
  31. Biophysics of HDL Dysfunction
    Mary G Sorci-Thomas; Fiscal Year: 2013
    ..Development of these novel assays will make it possible, for the first time, to perform large clinical studies to fully test the idea that dysfunctional HDL is better indicator of atherosclerotic risk. ..
  32. Unraveling the Genetic Architecture of Very High HDL cholesterol though Transcrip
    Michael Miller; Fiscal Year: 2010
    ..It is hoped that the identification of novel genes implicated in high HDL states that are associated with longevity will facilitate the development of novel therapies aimed at reducing heart disease. ..
  33. CELLULAR AND MOLECULAR BIOLOGY OF LIPOPROTEIN METABOLISM
    Michael C Phillips; Fiscal Year: 2013
    ..The reasons for this protective effect are not understood fully and this project seeks to uncover the molecular mechanisms underlying the beneficial properties of HDL. ..
  34. Postprandial Vitamin A
    WILLIAM BLANER; Fiscal Year: 2009
    ..Specific Aim 5 will define the role of RBP in facilitating vitamin A mobilization for adipocytes and delineating how this is linked to the proposed role of adipose-derived RBP in impairing insulin responsiveness ..
  35. Host sphingolipids and fungal infection
    Maurizio Del Poeta; Fiscal Year: 2011
    ..The studies that we propose in this application will reveal new regulatory mechanisms involved in the killing of Cn by neutrophils. We will identify the nexus between SMS, PKD and infection. ..
  36. Function and Inhibition of Plasmodium Lipid Decarboxylases
    Choukri Ben Mamoun; Fiscal Year: 2013
    ..falciparum development as well as fostering the design of specific inhibitors. This work will provide new therapeutic insights for combating a disease that affects 250 million people worldwide and causes 1 million deaths each year. ..
  37. De Novo Lipogenesis and Metabolic Disease
    Clay F Semenkovich; Fiscal Year: 2013
    ..By establishing compartmentalized FAS as a mediator of physiological signals, this project could improve human health by identifying novel therapeutic targets for a wide range of metabolic disorders associated with diabetes and obesity. ..
  38. STRUCTURE AND FUNCTION OF PHOSPHOETHANOLAMINE METHYLTRANSFERASES: NEW ANTI-PARAS
    JOSEPH MARTIN JEZ; Fiscal Year: 2013
    ....
  39. Phosphatidylcholine biosynthesis and miltefosine mode of action in Leishmania
    Rachel Zufferey; Fiscal Year: 2010
    ..The proposed research is significant, because it is expected to advance our understanding of phosphatidylcholine biogenesis in Leishmania and of miltefosine's mode of action. ..
  40. Phospholipase D1 in Alzheimer???s Precursor Protein trafficking and processing
    KIMBERLY SHAUNTAE POINT DU JOUR; Fiscal Year: 2013
    ....
  41. Homeostasis of the ER in Differentiating B-cells
    Robert A Barrington; Fiscal Year: 2013
    ..Understanding how the UPR works in normal cellular processes is necessary for rational exploration of drugs to modulate this pathway in disease states. ..
  42. The Role of Phospholipid Turnover in Membrane Protein Function
    JANA L VOGT; Fiscal Year: 2013
    ..In addition, the lipid requirements of a PM permease whose activity is reduced in response to loss of Ypk1 or Plb1 will be examined using unilamellar lipid vesicles. ..
  43. Mechanisms of neurodegeneration and brain iron accumulation
    Zhongmin Alex Ma; Fiscal Year: 2010
    ..Previously we rep rted that inhibition of iPLA2 results in the increase of cell-membranous phosphatidylcholines containin polyunsaturated fatty acid residues that induces phosphorylation of p53 through activation of ..
  44. Aspirin-PC for Chemoprevention of Colorectal Cancer
    Lenard M Lichtenberger; Fiscal Year: 2013
    ..These studies, therefore will establish the feasibility of using PL2200 for chemoprevention while lowering the risk of adverse GI events, resulting in the development of an efficacious and safe drug for prevention of colorectal cancer. ..
  45. TNF-alpha Control of a Surfactant Enzyme in Lung Injury
    Rama K Mallampalli; Fiscal Year: 2010
    ..abstract_text> ..
  46. PHOSPHOLIPID DYNAMICS IN MEMBRANE ASSEMBLY
    Dennis R Voelker; Fiscal Year: 2012
    ..This fundamental work is most relevant to areas of human disease concerned with control of cell growth and mitochondrial dysfunction, such as cancer and heart disease. ..
  47. LPCAT1 is essential for perinatal lung function and survival
    John M Shannon; Fiscal Year: 2013
    ....
  48. Septic Lung Injury and Surfactant
    Rama K Mallampalli; Fiscal Year: 2013
    ..Execution of studies outlined in this application will provide a major advance in the conceptual framework for how sepsis causes lung impairment by altering surfactant production. ..
  49. Dynamics of LCAT activation and lipoprotein remodeling
    Jere P Segrest; Fiscal Year: 2013
    ..abstract_text> ..
  50. Imaging of atheroma macrophage activities by rational liposomal shell design
    Patrick Kee; Fiscal Year: 2013
    ..If successful, this will compliment existing imaging technologies and allow better characterization of coronary lesions and patient management. ..
  51. Mechanisms of Lung Phospholipid Homeostasis
    Rama K Mallampalli; Fiscal Year: 2012
    ..Execution of these studies will lay the groundwork for a significant mechanistic advance with regard to the pathobiology of surfactant metabolism and mechanisms for alveolar homeostasis during inflammatory lung injury. ..
  52. Phospholipase D proteins in immunoreceptor-mediated signaling
    Weiguo Zhang; Fiscal Year: 2013
    ..This study will also facilitate the identification of intracellular targets for drug design to modulate leukocyte function during infection, transplantation, and treatment of cancer. ..
  53. Structural basis for HIV-1 Gag interactions with cellular constituents
    JAMIL SUBHI SAAD; Fiscal Year: 2013
    ..The proposed studies will help in understanding the precise molecular mechanism of HIV Gag trafficking and assembly, which may aid in the development of new strategies for antiviral drug design. ..
  54. PHOSPHOLIPIDS IN GOLGI SECRETORY FUNCTION
    Vytas A Bankaitis; Fiscal Year: 2013
    ....
  55. Apolipoprotein A-I structure in high density lipoproteins
    W Sean Davidson; Fiscal Year: 2013
    ....
  56. Clinical Evaluation of GI Safer Naproxen for the Treatment of Osteoarthritis
    UPENDRA K MARATHI; Fiscal Year: 2010
    ....
  57. Development of a Liposome Doxorubicin Product Drug Release Assay
    Peter Working; Fiscal Year: 2013
    ..abstract_text> ..
  58. Adenoviral Induced Acute Lung Injury and Surfactant
    Rama K Mallampalli; Fiscal Year: 2010
    ....
  59. Cone opsins in photoreceptor degeneration
    Yingbin Fu; Fiscal Year: 2013
    ..We expect these studies will define the mechanism on why S-cones degenerate faster than M-cones in RPE65/LRAT-LCA. ..
  60. Synthesis and Application of Novel Apolipoprotein Mimetics
    M Reza Ghadiri; Fiscal Year: 2012
    ..Our results may establish a platform for potential therapeutic agents to treat atherosclerosis by the regression of atherosclerotic plaque in vivo. ..
  61. The effect of macrophage sphingomyelin synthase (SMS) on atherosclerosis
    Xian Cheng Jiang; Fiscal Year: 2010
    ....
  62. PC-NSAIDs for Chemoprevention of Colorectal Cancer
    Lenard M Lichtenberger; Fiscal Year: 2013
    ....
  63. Function of the Nuclear Receptor LRH-1
    David D Moore; Fiscal Year: 2010
    ..They also show antidiabetic effects, and a particular goal is to explore how they work and whether they have potential therapeutic utility. ..
  64. MECHANISM OF TRH EFFECTS IN FETAL LUNG DEVELOPMENT
    UDAY DEVASKAR; Fiscal Year: 1999
    ....
  65. Phospholipid Nanocarriers Improve Chemotherapy by Targeted Drug Delivery
    Hayat Onyuksel; Fiscal Year: 2010
    ....
  66. MOLECULAR PHYSIOLOGY OF BILIARY LIPID SECRETION
    David Cohen; Fiscal Year: 2000
    ..mechanisms whereby bile salts promote biliary secretion of cholesterol together with highly specific phosphatidylcholines. Preliminary studies in model systems suggest that bile salts in submicellar concentrations promote ..
  67. MECHANISM AND ROLE OF MICROSOMAL ETHANOL OXIDATION
    Charles Lieber; Fiscal Year: 2001
    ..Such information is aimed at elucidating key aspects of alcohol mediated pathology in a way which may eventually affect alcohol related medical complications, their prevention and treatment. ..
  68. GI-Safer Naproxen Formulation R&D for the Treatment of Osteoarthritis and Related
    Upendra Marathi; Fiscal Year: 2009
    ..abstract_text> ..
  69. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  70. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
    ..All of the preliminary data was collected by undergraduates and the funding of this project would involve 15 to 20 undergraduates in meaningful research over the three-year term of the grant. [unreadable] [unreadable]..
  71. DOES PULMONARY SURFACTANT REACT WITH INHALED POLLUTANTS?
    BARBARA FINLAYSON PITTS; Fiscal Year: 1993
    ..The major surface-active components are phosphatidylcholines (PC), in which as much as 30% of the fatty acids are unsaturated, presenting potential reaction sites for ..
  72. EFFECT OF N-3 FATTY ACIDS ON HDL LIPID INTERACTION
    John Parks; Fiscal Year: 1992
    ..In addition, emphasis will be placed on the investigation of effects of n-3 fatty acid enrichment in PL species with regard to apoprotein binding to monolayers and recombinant particle structure...