glycerol

Summary

Summary: A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.

Top Publications

  1. pmc Osmotic stress signaling and osmoadaptation in yeasts
    Stefan Hohmann
    Department of Cell and Molecular Biology Microbiology, Goteborg University, S 405 30 Goteborg, Sweden
    Microbiol Mol Biol Rev 66:300-72. 2002
  2. ncbi Glycerol: a promising and abundant carbon source for industrial microbiology
    Gervásio Paulo da Silva
    Department of Education, Bahia State University Uneb, BR 407, Km 127, Senhor do Bonfim, BA, 48970 000, Brazil
    Biotechnol Adv 27:30-9. 2009
  3. pmc The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation
    R Ansell
    Department of General and Marine Microbiology, Gothenburg University, Sweden
    EMBO J 16:2179-87. 1997
  4. ncbi The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes
    M Rep
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    J Biol Chem 275:8290-300. 2000
  5. ncbi In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production
    Christoffer Bro
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs, Lyngby, Denmark
    Metab Eng 8:102-11. 2006
  6. pmc A systems-level analysis of perfect adaptation in yeast osmoregulation
    Dale Muzzey
    Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 138:160-71. 2009
  7. ncbi Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol
    Yanping Zhang
    Department of Chemical Engineering, Tsinghua University, Beijing, China
    Metab Eng 11:101-6. 2009
  8. ncbi Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol
    Chelladurai Rathnasingh
    Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609 735, Republic of Korea
    Biotechnol Bioeng 104:729-39. 2009
  9. pmc GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway
    J Albertyn
    Department of Microbiology and Biochemistry, Faculty of Science, University of the Orange Free State, Bloemfontein, South Africa
    Mol Cell Biol 14:4135-44. 1994
  10. pmc Borrelia burgdorferi requires glycerol for maximum fitness during the tick phase of the enzootic cycle
    Christopher J Pappas
    Department of Microbiology and Immunology, New York Medical College, Valhalla, United States of America
    PLoS Pathog 7:e1002102. 2011

Detail Information

Publications352 found, 100 shown here

  1. pmc Osmotic stress signaling and osmoadaptation in yeasts
    Stefan Hohmann
    Department of Cell and Molecular Biology Microbiology, Goteborg University, S 405 30 Goteborg, Sweden
    Microbiol Mol Biol Rev 66:300-72. 2002
    ..osmolarity, yeast cells rapidly stimulate a mitogen-activated protein (MAP) kinase cascade, the high-osmolarity glycerol (HOG) pathway, which orchestrates part of the transcriptional response...
  2. ncbi Glycerol: a promising and abundant carbon source for industrial microbiology
    Gervásio Paulo da Silva
    Department of Education, Bahia State University Uneb, BR 407, Km 127, Senhor do Bonfim, BA, 48970 000, Brazil
    Biotechnol Adv 27:30-9. 2009
    ..replace petroleum diesel, as it is produced from animal fats and vegetable oils, which generate about 10% (w/w) glycerol as the main by-product...
  3. pmc The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation
    R Ansell
    Department of General and Marine Microbiology, Gothenburg University, Sweden
    EMBO J 16:2179-87. 1997
    The two homologous genes GPD1 and GPD2 encode the isoenzymes of NAD-dependent glycerol 3-phosphate dehydrogenase in the yeast Saccharomyces cerevisiae...
  4. ncbi The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes
    M Rep
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    J Biol Chem 275:8290-300. 2000
    ..encode proteins that presumably contribute to protection against different types of damage or encode enzymes in glycerol, trehalose, and glycogen metabolism...
  5. ncbi In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production
    Christoffer Bro
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs, Lyngby, Denmark
    Metab Eng 8:102-11. 2006
    ..to score a number of strategies for metabolic engineering of the redox metabolism that will lead to decreased glycerol and increased ethanol yields on glucose under anaerobic conditions...
  6. pmc A systems-level analysis of perfect adaptation in yeast osmoregulation
    Dale Muzzey
    Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 138:160-71. 2009
    ..By monitoring multiple system quantities (e.g., cell volume, Hog1, glycerol) and using varied input waveforms (e.g...
  7. ncbi Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol
    Yanping Zhang
    Department of Chemical Engineering, Tsinghua University, Beijing, China
    Metab Eng 11:101-6. 2009
    ..the effect of introducing a heterogeneous NADH regeneration system into Klebsiella oxytoca on cell growth and glycerol metabolism. Expression of fdh gene from Candida boidinii in K...
  8. ncbi Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol
    Chelladurai Rathnasingh
    Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609 735, Republic of Korea
    Biotechnol Bioeng 104:729-39. 2009
    ..Our previous study demonstrated the feasibility of producing 3-HP from glycerol using the recombinant Escherichia coli SH254 expressing glycerol dehydratase (DhaB) and aldehyde dehydrogenase (..
  9. pmc GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway
    J Albertyn
    Department of Microbiology and Biochemistry, Faculty of Science, University of the Orange Free State, Bloemfontein, South Africa
    Mol Cell Biol 14:4135-44. 1994
    ..e., an increase in osmolarity of the growth medium, by enhanced production and intracellular accumulation of glycerol as a compatible solute...
  10. pmc Borrelia burgdorferi requires glycerol for maximum fitness during the tick phase of the enzootic cycle
    Christopher J Pappas
    Department of Microbiology and Immunology, New York Medical College, Valhalla, United States of America
    PLoS Pathog 7:e1002102. 2011
    ..contains an operon that is predicted to encode proteins that would mediate the uptake and conversion of glycerol to dihydroxyacetone phosphate...
  11. pmc Gpd1 and Gpd2 fine-tuning for sustainable reduction of glycerol formation in Saccharomyces cerevisiae
    Georg Hubmann
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31 bus 2438, B 3001 Heverlee, Flanders, Belgium
    Appl Environ Microbiol 77:5857-67. 2011
    Gpd1 and Gpd2 are the two isoforms of glycerol 3-phosphate dehydrogenase (GPDH), which is the rate-controlling enzyme of glycerol formation in Saccharomyces cerevisiae...
  12. ncbi Metabolic activities of biotechnological interest in Yarrowia lipolytica grown on glycerol in repeated batch cultures
    Anna Makri
    Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, 26504 GR, Greece
    Bioresour Technol 101:2351-8. 2010
    The growth of Yarrowia lipolytica on glycerol was studied in bioreactor repeated batch cultures and three distinct phases, namely biomass production phase, lipogenic phase and citric acid production phase were identified during growth ..
  13. pmc Tor1/Sch9-regulated carbon source substitution is as effective as calorie restriction in life span extension
    Min Wei
    Andrus Gerontology Center, University of Southern California, Los Angeles, CA, USA
    PLoS Genet 5:e1000467. 2009
    ..a common set of genes implicated in a metabolic switch from the TCA cycle and respiration to glycolysis and glycerol biosynthesis...
  14. ncbi Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking
    I S Pretorius
    Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch, ZA 7600, South Africa
    Yeast 16:675-729. 2000
    ..However, the staggering potential advantages of improved wine yeasts to both the winemaker and consumer in the third millennium are pointed out...
  15. ncbi The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress
    A K Pahlman
    Department of Cell and Molecular Biology, Microbiology, Goteborg University, Box 463, SE 40530 Goteborg, Sweden
    J Biol Chem 276:3555-63. 2001
    We have characterized the strongly homologous GPP1/RHR2 and GPP2/HOR2 genes, encoding isoforms of glycerol 3-phosphatase...
  16. pmc Modelling reveals novel roles of two parallel signalling pathways and homeostatic feedbacks in yeast
    Jörg Schaber
    Institute for Experimental Internal Medicine, Medical Faculty, Otto von Guericke University, Magdeburg, Germany
    Mol Syst Biol 8:622. 2012
    The high osmolarity glycerol (HOG) pathway in yeast serves as a prototype signalling system for eukaryotes...
  17. ncbi Cell wall integrity and high osmolarity glycerol pathways are required for adaptation of Alternaria brassicicola to cell wall stress caused by brassicaceous indolic phytoalexins
    Aymeric Joubert
    UMR PAVE No 77, IFR 149 QUASAV, 2 Bd Lavoisier, F 49045 Angers Cedex, France
    Cell Microbiol 13:62-80. 2011
    ..that activation of a cellular response to this damage requires cell wall integrity (CWI) and the high osmolarity glycerol (HOG) pathways...
  18. ncbi Chitosan-glycerol phosphate/blood implants elicit hyaline cartilage repair integrated with porous subchondral bone in microdrilled rabbit defects
    C D Hoemann
    Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, Canada Institute of Biomedical Engineering, Ecole Polytechnique, Montreal, QC, Canada
    Osteoarthritis Cartilage 15:78-89. 2007
    ..are repaired with more hyaline cartilage when the defect is treated with in situ-solidified implants of chitosan-glycerol phosphate (chitosan-GP) mixed with autologous whole blood...
  19. pmc Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli
    M Kenyon Applebee
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093, USA
    J Biol Chem 286:23150-9. 2011
    Herein we measure the effect of four adaptive non-synonymous mutations to the glycerol kinase (glpK) gene on catalytic function and regulation, to identify changes that correlate to increased fitness in glycerol media...
  20. ncbi A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli
    Ramon Gonzalez
    Department of Chemical and Biomolecular Engineering, Rice University, P O Box 1892, Houston, TX 77251 1892, USA
    Metab Eng 10:234-45. 2008
    Anaerobic fermentation of glycerol in the Enterobacteriaceae family has long been considered a unique property of species that synthesize 1,3-propanediol (1,3-PDO)...
  21. ncbi Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects
    A Chevrier
    Chemical Engineering, Ecole Polytechnique, PO Box 6079, Station Centre Ville, Montreal, Quebec, Canada
    Osteoarthritis Cartilage 15:316-27. 2007
    ..We have shown previously that autologous blood can be mixed with a polymer solution containing chitosan in a glycerol phosphate (GP) buffer (chitosan-GP), and that implantation of this polymer/blood composite onto marrow-stimulated ..
  22. ncbi Neuronal uptake and metabolism of glycerol and the neuronal expression of mitochondrial glycerol-3-phosphate dehydrogenase
    Nga Huynh Tran Nguyen
    Norwegian Defence Research Establishment, N 2027 Kjeller, Norway
    J Neurochem 85:831-42. 2003
    b>Glycerol is effective in the treatment of brain oedema but it is unclear if this is due solely to osmotic effects of glycerol or whether the brain may metabolize glycerol...
  23. ncbi Lipids containing polyunsaturated fatty acids synthesized by zygomycetes grown on glycerol
    Stamatia Bellou
    Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece
    Appl Biochem Biotechnol 166:146-58. 2012
    Several strains of Zygomycetes cultivated on glycerol produced mycelia rich in lipids containing higher amounts of neutral lipids (NL) than glycolipids plus sphingolipids and phospholipids (P), while biosynthesis of P in Mortierella ..
  24. ncbi Mechanisms of protein stabilization and prevention of protein aggregation by glycerol
    Vincent Vagenende
    Singapore MIT Alliance, National University of Singapore, 4 Engineering Drive 3, Singapore 117576
    Biochemistry 48:11084-96. 2009
    The stability of proteins in aqueous solution is routinely enhanced by cosolvents such as glycerol. Glycerol is known to shift the native protein ensemble to more compact states...
  25. pmc Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
    Jörg Schaber
    Theoretical Biophysics, Humboldt University, Invaliden Str 42, 10115 Berlin, Germany
    Eur Biophys J 39:1547-56. 2010
    ..The parameterized turgor models showed that activation of the osmosensing high osmolarity glycerol (HOG) signaling pathway correlates with turgor loss in a 1:1 relationship...
  26. pmc Pyruvate dehydrogenase kinase 4: regulation by thiazolidinediones and implication in glyceroneogenesis in adipose tissue
    Thomas Cadoudal
    Institut National de la Santé et de la Recherche Médicale Unité Mixte de Recherche S 747, Universite Paris Descartes, Centre Universitaire des Saints Peres, Paris, France
    Diabetes 57:2272-9. 2008
    ..Thiazolidinediones activate glyceroneogenesis. We studied the regulation by rosiglitazone of PDK2 and PDK4 isoforms and tested the hypothesis that glyceroneogenesis could be controlled by PDK...
  27. ncbi The high-osmolarity glycerol (HOG) and cell wall integrity (CWI) signalling pathways interplay: a yeast dialogue between MAPK routes
    José Manuel Rodriguez-Peña
    Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid UCM, IRYCIS, 28040 Madrid, Spain
    Yeast 27:495-502. 2010
    Two mitogen-activated protein kinase (MAPK) pathways, viz. the high-osmolarity glycerol (HOG) and the cell wall integrity (CWI) pathways, regulate stress responses in the yeast Saccharomyces cerevisiae...
  28. ncbi Yeast osmoregulation
    Stefan Hohmann
    Department of Cell and Molecular Biology, Goteborg University, Goteborg, Sweden
    Methods Enzymol 428:29-45. 2007
    ..Key to yeast osmoregulation is the production and accumulation of the compatible solute glycerol, which is partly controlled by the high osmolarity glycerol (HOG) signaling system...
  29. pmc Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA
    Ulf B Christensen
    Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nucleic Acids Res 30:4918-25. 2002
    ..The phosphoramidite of (S)-1-O-(4, 4'-dimethoxytriphenylmethyl)-3-O-(1-pyrenylmethyl)glycerol, an intercalating pseudo-nucleotide (IPN), was synthesised and by standard methods inserted into 7mer and 13mer ..
  30. ncbi Glycerol hyperhydration improves cycle time trial performance in hot humid conditions
    S Hitchins
    Department of Physiology and Pharmacology, James Cook University, Townsville, QLD, Australia
    Eur J Appl Physiol Occup Physiol 80:494-501. 1999
    ..Each trial was preceded by ingestion of either a glycerol solution [1 g x kg(-1) body mass (BM) in a diluted carbohydrate (CHO)-electrolyte drink] or a placebo of equal ..
  31. ncbi Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture
    Seraphim Papanikolaou
    Laboratory of General and Agricultural Microbiology, Department of Agricultural Biotechnology, Agricultural University of Athens, Votanikos, Greece
    Bioresour Technol 82:43-9. 2002
    Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol used as sole carbon substrate...
  32. ncbi Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF
    B L de Groot
    Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 294:2353-7. 2001
    Real time" molecular dynamics simulations of water permeation through human aquaporin-1 (AQP1) and the bacterial glycerol facilitator GlpF are presented...
  33. ncbi Control of high osmolarity signalling in the yeast Saccharomyces cerevisiae
    Stefan Hohmann
    Department of Cell and Molecular Biology, University of Gothenburg, Goteborg, Sweden
    FEBS Lett 583:4025-9. 2009
    ..The yeast HOG (High Osmolarity Glycerol) response pathway mediates cellular adaptation to hyperosmotic stress...
  34. ncbi High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica
    Waldemar Rymowicz
    Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50 375, Wroclaw, Poland
    Biotechnol Lett 31:377-80. 2009
    An acetate-negative mutant of Yarrowia lipolytica Wratislavia K1 was selected that, when grown with 300 g raw glycerol l(-1) at pH 3, produced 170 g erythritol l(-1) after 7 days, corresponding to a 56% yield and a productivity of 1 g l(-..
  35. ncbi Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry
    Syed Shams Yazdani
    Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
    Curr Opin Biotechnol 18:213-9. 2007
    ..b>Glycerol-rich streams generated in large amounts by the biofuels industry, especially during the production of biodiesel, ..
  36. ncbi High osmolarity glycerol (HOG) pathway-induced phosphorylation and activation of 6-phosphofructo-2-kinase are essential for glycerol accumulation and yeast cell proliferation under hyperosmotic stress
    Hassan Dihazi
    Department of Nephrology and Rheumatology, University Hospital Gottingen, Robert Koch Strasse 40, D 37075 Gottingen, Germany
    J Biol Chem 279:23961-8. 2004
    ..The high osmolarity glycerol (HOG) MAPK pathway is activated by increased environmental osmolarity and results in a rise of the cellular ..
  37. pmc Point mutations in the aromatic/arginine region in aquaporin 1 allow passage of urea, glycerol, ammonia, and protons
    Eric Beitz
    Department of Pharmaceutical Biochemistry, University of Tubingen, Morgenstelle 8, D 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 103:269-74. 2006
    ..Phe-56 and His-180 (AQP1-F56A/H180A) enlarged the maximal diameter of the ar/R constriction 3-fold and enabled glycerol and urea to pass...
  38. pmc Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli
    Xueming Tang
    Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, People s Republic of China
    Appl Environ Microbiol 75:1628-34. 2009
    ..the production of 1,3-PD in Clostridium butyricum, dhaB1 and dhaB2, which encode the vitamin B(12)-independent glycerol dehydratase DhaB1 and its activating factor, DhaB2, respectively, were tandemly arrayed with the E...
  39. ncbi Thermal stability of alpha-amylase in aqueous cosolvent systems
    Jay Kant Yadav
    Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore 570020, India
    J Biosci 34:377-87. 2009
    ..of alpha-amylase were studied in the presence of different concentrations of trehalose, sorbitol, sucrose and glycerol. The optimum temperature of the enzyme was found to be 50 +/- 2 degrees C...
  40. ncbi Modulation of the glycerol and ethanol syntheses in the yeast Saccharomyces kudriavzevii differs from that exhibited by Saccharomyces cerevisiae and their hybrid
    F Noé Arroyo-López
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Edifici d Instituts, Parc Científic de Paterna, PO Box 22085, E 46071 Valencia, Spain
    Food Microbiol 27:628-37. 2010
    In the last years there is an increasing demand to produce wines with higher glycerol levels and lower ethanol contents...
  41. pmc Aquaporin-9 protein is the primary route of hepatocyte glycerol uptake for glycerol gluconeogenesis in mice
    Sabina Jelen
    From the Water and Salt Research Center, Department of Biomedicine, Aarhus University, Wilhelm Meyers Alle, DK 8000 Aarhus C, Denmark
    J Biol Chem 286:44319-25. 2011
    It has been hypothesized that aquaporin-9 (AQP9) is part of the unknown route of hepatocyte glycerol uptake...
  42. pmc Reciprocal phosphorylation of yeast glycerol-3-phosphate dehydrogenases in adaptation to distinct types of stress
    Yong Jae Lee
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut, USA
    Mol Cell Biol 32:4705-17. 2012
    ..Saccharomyces cerevisiae has two homologous glycerol-3-phosphate dehydrogenases, Gpd1 and Gpd2, that are required to endure various stresses, including hyperosmotic ..
  43. ncbi Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding
    Narendar K Khatri
    Institute for Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    Biotechnol J 6:452-62. 2011
    ..To address the problem, the adaptation phase with growth-limiting feeding of glycerol before the production phase was optimized...
  44. ncbi Ethanol production from biodiesel-derived crude glycerol by newly isolated Kluyvera cryocrescens
    Won Jae Choi
    Institute of Chemical and Engineering Sciences, Agency for Science, Technology and Research A STAR, 1 Pesek Road, Jurong Island, Singapore
    Appl Microbiol Biotechnol 89:1255-64. 2011
    The rapidly expanding market for biodiesel has increased the supply and reduced the cost of glycerol, making it an attractive sustainable feed stock for the fuel and chemical industry...
  45. ncbi Optimization of medium compositions favoring butanol and 1,3-propanediol production from glycerol by Clostridium pasteurianum
    Chuloo Moon
    Clean Energy Research Center, Korea Institute of Science and Technology, 39 1 Hawolgok Dong, Seongbuk gu, Seoul 136 791, Republic of Korea
    Bioresour Technol 102:10561-8. 2011
    Medium compositions favoring butanol and 1,3-propanediol (1,3-PDO) production from glycerol by Clostridium pasteurianum DSM525 were investigated using statistical experimental designs...
  46. ncbi Evolutionary differences in food preference rely on Gr64e, a receptor for glycerol
    Zev Wisotsky
    Interdepartmental Neuroscience Program, University of California, Riverside, California, USA
    Nat Neurosci 14:1534-41. 2011
    ..We found that Gr64e is required for neuronal and behavioral responses to glycerol, an abundant component of growing yeast and fermentation products...
  47. pmc Quantitative analysis of glycerol accumulation, glycolysis and growth under hyper osmotic stress
    Elzbieta Petelenz-Kurdziel
    Department of Chemistry and Molecular Biology Microbiology, University of Gothenburg, Goteborg, Sweden
    PLoS Comput Biol 9:e1003084. 2013
    ..Saccharomyces cerevisiae adapts to hyperosmotic stress by activating the HOG signaling cascade, which controls glycerol accumulation...
  48. ncbi Glycerol dissimilation and its regulation in bacteria
    E C Lin
    Annu Rev Microbiol 30:535-78. 1976
  49. ncbi Modulation of phospholipase D-mediated phosphatidylglycerol formation by differentiating agents in primary mouse epidermal keratinocytes
    Xiangjian Zheng
    Program in Cell Signaling, Institute of Molecular Medicine and Genetics, Department of Medicine Dermatology, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912 2630, USA
    Biochim Biophys Acta 1643:25-36. 2003
    ..In this report, PLD was demonstrated to utilize the physiological alcohol glycerol to form phosphatidylglycerol (PG) in vitro...
  50. pmc Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process
    Julien Pagliardini
    Universite de Toulouse, INSA, UPS, INP, LISBP, 135 av de Rangueil, F 31077 Toulouse, France
    Microb Cell Fact 9:36. 2010
    b>Glycerol is the major by-product accounting for up to 5% of the carbon in Saccharomyces cerevisiae ethanolic fermentation. Decreasing glycerol formation may redirect part of the carbon toward ethanol production...
  51. pmc Phase 2 comparison of a novel ammonia scavenging agent with sodium phenylbutyrate in patients with urea cycle disorders: safety, pharmacokinetics and ammonia control
    Brendan Lee
    Baylor College of Medicine, Houston, TX, USA
    Mol Genet Metab 100:221-8. 2010
    b>Glycerol phenylbutyrate (glyceryl tri (4-phenylbutyrate)) (GPB) is being studied as an alternative to sodium phenylbutyrate (NaPBA) for the treatment of urea cycle disorders (UCDs)...
  52. pmc Sphingolipids regulate the yeast high-osmolarity glycerol response pathway
    Mirai Tanigawa
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
    Mol Cell Biol 32:2861-70. 2012
    The yeast high-osmolarity glycerol response (HOG) mitogen-activated protein (MAP) kinase pathway is activated in response to hyperosmotic stress via two independent osmosensing branches, the Sln1 branch and the Sho1 branch...
  53. pmc Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells
    Jakub Orzechowski Westholm
    Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
    PLoS ONE 7:e31577. 2012
    ..Significantly, Gis1 and Rph1 control a number of genes involved in acetate and glycerol formation, metabolites that have been implicated in aging...
  54. pmc GPR-4 is a predicted G-protein-coupled receptor required for carbon source-dependent asexual growth and development in Neurospora crassa
    Liande Li
    Department of Plant Pathology, 1415 Boyce Hall, 900 University Avenue, University of California, Riverside, Riverside, CA 92521, USA
    Eukaryot Cell 5:1287-300. 2006
    ..Deltagpr-4 mutants have reduced mass accumulation compared to the wild type when cultured on high levels of glycerol, mannitol, or arabinose. The defect is most severe on glycerol and is cell density dependent...
  55. pmc Ammonia control and neurocognitive outcome among urea cycle disorder patients treated with glycerol phenylbutyrate
    George A Diaz
    Mount Sinai School of Medicine, Department of Genetics and Genomic Sciences, Department of Pediatrics, New York, NY, USA
    Hepatology 57:2171-9. 2013
    b>Glycerol phenylbutyrate is under development for treatment of urea cycle disorders (UCDs), rare inherited metabolic disorders manifested by hyperammonemia and neurological impairment...
  56. ncbi A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol
    Li Bo Yang
    Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China
    Bioresour Technol 151:120-7. 2014
    ..on erythritol and mannitol production by an osmophilic yeast strain of Yarrowia lipolytica CICC 1675 using glycerol as the sole carbon source was investigated...
  57. ncbi Production of 1,2-propanediol from glycerol in Saccharomyces cerevisiae
    Joon Young Jung
    Department of Chemical and Biological Engineering, Korea University, Seoul 136 713, Korea
    J Microbiol Biotechnol 21:846-53. 2011
    b>Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate...
  58. pmc Electrospray ionization multiple-stage linear ion-trap mass spectrometry for structural elucidation of triacylglycerols: assignment of fatty acyl groups on the glycerol backbone and location of double bonds
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 21:657-69. 2010
    ..structural elucidation of triacylglycerol (TAG) including (1) assignment the fatty acid substituents on the glycerol backbone and (2) location of the double bond(s) on the unsaturated fatty acyl groups is reported...
  59. ncbi Crude glycerol-based production of amino acids and putrescine by Corynebacterium glutamicum
    Tobias M Meiswinkel
    Chair of Genetics of Prokaryotes, Bielefeld University, D 33615 Bielefeld, Germany
    Bioresour Technol 145:254-8. 2013
    Corynebacterium glutamicum possesses genes for glycerol kinase and glycerol-3-phosphate dehydrogenase that were shown to support slow growth with glycerol only when overexpressed from a plasmid...
  60. ncbi Functional characterization of three aquaglyceroporins from Trypanosoma brucei in osmoregulation and glycerol transport
    Björn Bassarak
    Interfaculty Institute for Biochemistry, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 27:411-20. 2011
    Previous studies using bloodstream form Trypanosoma brucei have shown that glycerol transport in this parasite occurs via specific membrane proteins, namely a glycerol transporter and glycerol channels [1]...
  61. pmc Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli
    O I Sanders
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Bacteriol 179:3365-7. 1997
    ..Sequence analysis showed that the TnphoA insertion was located in the glpF gene, coding for the glycerol facilitator GlpF. The mutant was shown to be defective in polyol transport by GlpF...
  62. ncbi Citric acid production from glycerol-containing waste of biodiesel industry by Yarrowia lipolytica in batch, repeated batch, and cell recycle regimes
    Waldemar Rymowicz
    Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, PL 50 375, Wroclaw, Poland
    Appl Microbiol Biotechnol 87:971-9. 2010
    ..6 g g(-1) and a productivity of 0.71 g l(-1) h(-1) during batch cultivation in the medium with glycerol-containing waste of biodiesel industry...
  63. pmc Expression of GUT1, which encodes glycerol kinase in Saccharomyces cerevisiae, is controlled by the positive regulators Adr1p, Ino2p and Ino4p and the negative regulator Opi1p in a carbon source-dependent fashion
    M Grauslund
    Danisco Biotechnology, Danisco A S, Langebrogade 1, DK 1001 Copenhagen K, Denmark
    Nucleic Acids Res 27:4391-8. 1999
    In Saccharomyces cerevisiae glycerol utilization is mediated by two enzymes, glycerol kinase (Gut1p) and mitochondrial glycerol-3-phosphate dehydrogenase (Gut2p)...
  64. ncbi Progressive adipocyte hypertrophy in aquaporin-7-deficient mice: adipocyte glycerol permeability as a novel regulator of fat accumulation
    Mariko Hara-Chikuma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 280:15493-6. 2005
    Aquaporin-7 (AQP7) is a water/glycerol transporting protein expressed in adipocyte plasma membranes. We report here remarkable age-dependent hypertrophy in adipocytes in AQP7-deficient mice...
  65. ncbi Aquaporin 3 colocates with phospholipase d2 in caveolin-rich membrane microdomains and is downregulated upon keratinocyte differentiation
    Xiangjian Zheng
    Program in Cell Signaling, Institute of Molecular Medicine and Genetics, Department of Medicine, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Invest Dermatol 121:1487-95. 2003
    Aquaporin 3 is a channel that transports both water and glycerol. Aquaporin 3-deficient mice exhibit skin defects, including decreased glycerol content and impairment of water holding capacity, barrier recovery, and wound healing...
  66. ncbi The cerebral extracellular release of glycerol, glutamate, and FGF2 is increased in older patients following severe traumatic brain injury
    Pekka Mellergård
    Department of Neurosurgery, University Hospital, Linkoping, Sweden
    J Neurotrauma 29:112-8. 2012
    ..The concentrations of glycerol, glutamate, lactate, pyruvate, and eight different cytokines (IL-1β, IL-6, IL-10, IL-8, MIP-1β, RANTES, FGF2, ..
  67. ncbi Microbial utilization of crude glycerol for the production of value-added products
    Rosemary Dobson
    Wits Industrial Biotechnology Consortium, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand Johannesburg, Johannesburg 2050, South Africa
    J Ind Microbiol Biotechnol 39:217-26. 2012
    ..For example, interest in biodiesel has undergone a revival in recent times. However, the disposal of crude glycerol contaminated with methanol, salts, and free fatty acids as a by-product of biodiesel production presents an ..
  68. pmc The peptidoglycan sacculus of Myxococcus xanthus has unusual structural features and is degraded during glycerol-induced myxospore development
    Nhat Khai Bui
    Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Bacteriol 191:494-505. 2009
    ..The sacculus was degraded during the transition from vegetative cells to glycerol-induced myxospores...
  69. ncbi Improving ethanol productivity by modification of glycolytic redox factor generation in glycerol-3-phosphate dehydrogenase mutants of an industrial ethanol yeast
    Zhong peng Guo
    The Key Laboratory of Industrial Biotechnology, Ministry of Education, Center for Bioresources and Bioenergy, School of Biotechnology, Jiangnan University, 214122, Wuxi, People s Republic of China
    J Ind Microbiol Biotechnol 38:935-43. 2011
    The GPD2 gene, encoding NAD(+)-dependent glycerol-3-phosphate dehydrogenase in an industrial ethanol-producing strain of Saccharomyces cerevisiae, was deleted...
  70. ncbi Novel bioactive glycerol-based lysophospholipids: new data -- new insight into their function
    Anna Grzelczyk
    Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4 10, 90 924 Lodz, Poland
    Biochimie 95:667-79. 2013
    ..Therefore, the present review is devoted to novel bioactive glycerol-based lysophospholipids and recent findings concerning their functions and possible signaling pathways regulating ..
  71. pmc An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)
    Jennifer L Reed
    Department of Bioengineering, University of California, San Diego, Gilman Drive, La Jolla, CA 92092, USA
    Genome Biol 4:R54. 2003
    ..The most comprehensive E. coli model to date (E. coli iJE660a GSM) accounts for 660 genes and includes 627 unique biochemical reactions...
  72. pmc Microbicide excipients can greatly increase susceptibility to genital herpes transmission in the mouse
    Thomas R Moench
    ReProtect, Inc, Baltimore, MD 21286 USA
    BMC Infect Dis 10:331. 2010
    ..However, excipients have not previously been tested in susceptibility models...
  73. pmc Prevention of skin tumorigenesis and impairment of epidermal cell proliferation by targeted aquaporin-3 gene disruption
    Mariko Hara-Chikuma
    Cardiovascular Research Institute, 1246 Health Sciences East Tower, Box 0521, University of California San Francisco, San Francisco, CA 94143 0521, USA
    Mol Cell Biol 28:326-32. 2008
    Aquaporin-3 (AQP3) is a water/glycerol-transporting protein expressed strongly at the plasma membranes of basal epidermal cells in skin. We found that human skin squamous cell carcinoma strongly overexpresses AQP3...
  74. pmc Mechanism of selectivity in aquaporins and aquaglyceroporins
    Jochen S Hub
    Computational Biomolecular Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 105:1198-203. 2008
    ..We studied the selectivity of aquaporin-1 (AQP1) and the bacterial glycerol facilitator, GlpF, for O(2), CO(2), NH(3), glycerol, urea, and water...
  75. ncbi Gene expression profiles and intracellular contents of stress protectants in Saccharomyces cerevisiae under ethanol and sorbitol stresses
    Tomohiro Kaino
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Appl Microbiol Biotechnol 79:273-83. 2008
    ..During various stresses, the yeast Saccharomyces cerevisiae induces glycerol or trehalose synthesis, but the fluctuations in gene expression and intracellular levels of proline in yeast are ..
  76. ncbi Production of arabitol from glycerol: strain screening and study of factors affecting production yield
    Srujana Koganti
    Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325 3906, USA
    Appl Microbiol Biotechnol 90:257-67. 2011
    b>Glycerol is a major by-product from biodiesel production, and developing new uses for glycerol is imperative to overall economics and sustainability of the biodiesel industry...
  77. ncbi Yarrowia lipolytica as a potential producer of citric acid from raw glycerol
    S Papanikolaou
    Laboratoire des Sciences du Genie Chimique, Vandoeuvre lès Nancy France
    J Appl Microbiol 92:737-44. 2002
    To study the biochemical response of Yarrowia lipolytica LGAM S(7)1 during growth on raw glycerol (the main by-product of bio-diesel production units) in order to produce metabolic products of industrial significance.
  78. pmc Structural context shapes the aquaporin selectivity filter
    David F Savage
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 107:17164-9. 2010
    ..contains pure water channels, whereas a second subfamily contains channels that conduct small alditols such as glycerol, in addition to water...
  79. ncbi Accumulation of a cocoa-butter-like lipid by Yarrowia lipolytica cultivated on agro-industrial residues
    Seraphim Papanikolaou
    Laboratoire des Sciences du Genie Chimique, rue du Bois de la Champelle, 54500, Vandoeuvre les Nancy, France
    Curr Microbiol 46:124-30. 2003
    ..on mixtures of saturated free fatty acids (an industrial derivative of animal fat called stearin), technical glycerol (the main by-product of bio-diesel production facilities), and glucose...
  80. ncbi When the stress of your environment makes you go HOG wild
    Patrick J Westfall
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    Science 306:1511-2. 2004
    ..mitogen-activated protein kinase cascade, known in budding yeast Saccharomyces cerevisiae as the high osmolarity glycerol (HOG) pathway...
  81. pmc Survival of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis frozen in skim milk- tryptone-glucose-glycerol medium
    Tarja Kaijalainen
    KTL, National Public Health Institute, Oulu, Helsinki, Finland
    J Clin Microbiol 42:412-4. 2004
    In STGG (skim milk, tryptone, glucose, glycerol) medium at -80 degrees C, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates survived for at least 3 years, and the same species have survived in ..
  82. ncbi The induction of trehalose and glycerol in Saccharomyces cerevisiae in response to various stresses
    Lili Li
    South China University of Technology, Guangzhou, PR China
    Biochem Biophys Res Commun 387:778-83. 2009
    Trehalose and glycerol have been implicated as potential stress protectants that accumulate in yeasts during various stress conditions...
  83. ncbi Gis1 is required for transcriptional reprogramming of carbon metabolism and the stress response during transition into stationary phase in yeast
    Nianshu Zhang
    Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Microbiology 155:1690-8. 2009
    ..In particular, Rim15p is required for the expression of genes involved in gluconeogenesis/glycolysis and glycerol biosynthesis only when ethanol is used as the carbon source...
  84. ncbi Association between elevated brain tissue glycerol levels and poor outcome following severe traumatic brain injury
    Tobias Clausen
    Department of Anesthesiology and Intensive Care Medicine, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    J Neurosurg 103:233-8. 2005
    b>Glycerol is considered to be a marker of cell membrane degradation and thus cellular lysis. Recently, it has become feasible to measure via microdialysis cerebral extracellular fluid (ECF) glycerol concentrations at the patient's bedside...
  85. pmc Continuous-flow enzyme assay on a microfluidic chip for monitoring glycerol secretion from cultured adipocytes
    Anna M Clark
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Anal Chem 81:2350-6. 2009
    ..coupled perfusion of cultured adipocytes with on-line fluorescence-based enzyme assay was developed to monitor glycerol secretions in real time from cultured adipocytes...
  86. ncbi An evolved Escherichia coli strain for producing hydrogen and ethanol from glycerol
    Hongbo Hu
    Department of Chemical Engineering, 220 Jack E Brown Building, Texas A and M University College Station, TX 77843 3122, USA
    Biochem Biophys Res Commun 391:1033-8. 2010
    b>Glycerol is an attractive feedstock for biofuels since it accumulates as a byproduct during biodiesel operations; hence, here we consider converting glycerol to hydrogen using the formate hydrogen lyase system of Escherichia coli which ..
  87. pmc Identification of positive regulators of the yeast fps1 glycerol channel
    Sara E Beese
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, Maryland, USA
    PLoS Genet 5:e1000738. 2009
    The yeast Fps1 protein is an aquaglyceroporin that functions as the major facilitator of glycerol transport in response to changes in extracellular osmolarity...
  88. pmc Glycerol metabolism and PrfA activity in Listeria monocytogenes
    Biju Joseph
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Gebäude E1, 97080 Wurzburg, Germany
    J Bacteriol 190:5412-30. 2008
    Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source...
  89. ncbi Acute IL-6 treatment increases fatty acid turnover in elderly humans in vivo and in tissue culture in vitro
    E W Petersen
    Copenhagen Muscle Research Center, Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 288:E155-62. 2005
    ..Moreover, IL-6 appears to be activating lipolysis independently of elevations in GH and/or cortisol and appears to be a potent catalyst for fat oxidation in muscle cells...
  90. ncbi Copper inhibits the water and glycerol permeability of aquaporin-3
    Marina Zelenina
    Department of Woman and Child Health, Karolinska Institutet, Stockholm S 171 77, Sweden
    J Biol Chem 279:51939-43. 2004
    ..Here we report that both water and glycerol permeability of human AQP3 is inhibited by copper. Inhibition is fast, dose-dependent, and reversible...
  91. ncbi Patch-clamp studies in the CNS illustrate a simple new method for obtaining viable neurons in rat brain slices: glycerol replacement of NaCl protects CNS neurons
    Jiang Hong Ye
    Department of Anesthesiology, New Jersey Medical School UMDNJ, 185 South Orange Avenue, Newark, NJ 07103 2714, USA
    J Neurosci Methods 158:251-9. 2006
    ..to use a modified artificial cerebrospinal fluid (ACSF) in which all NaCl was replaced initially by equi-osmotic glycerol. This modified glycerol-based ACSF was used during slice preparation...
  92. pmc A member of the sugar transporter family, Stl1p is the glycerol/H+ symporter in Saccharomyces cerevisiae
    Célia Ferreira
    Carlsberg Laboratory, Copenhagen Valby, Denmark
    Mol Biol Cell 16:2068-76. 2005
    b>Glycerol and other polyols are used as osmoprotectants by many organisms. Several yeasts and other fungi can take up glycerol by proton symport...
  93. ncbi Anaerobic fermentation of glycerol by Escherichia coli: a new platform for metabolic engineering
    Yandi Dharmadi
    Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77251 1892, USA
    Biotechnol Bioeng 94:821-9. 2006
    The worldwide surplus of glycerol generated as inevitable byproduct of biodiesel fuel and oleochemical production is resulting in the shutdown of traditional glycerol-producing/refining plants and new applications are needed for this now ..
  94. ncbi Glycerol hyperhydration fails to improve endurance performance and thermoregulation in humans in a warm humid environment
    Frank E Marino
    Human Movement Studies Unit and Human Performance Laboratory, Charles Sturt University, NSW 2795 Bathurst, Australia
    Pflugers Arch 446:455-62. 2003
    It is equivocal whether glycerol hyperhydration improves exercise performance and thermoregulation in the heat...
  95. pmc Evolutionary programming as a platform for in silico metabolic engineering
    Kiran Raosaheb Patil
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs, Lyngby, Denmark
    BMC Bioinformatics 6:308. 2005
    ....
  96. pmc Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol
    Cong T Trinh
    Department of Chemical Engineering and Materials Science and BioTechnology Institute, University of Minnesota, 240 Gortner Laboratory, 1479 Gortner Ave, St Paul, MN 55108, USA
    Appl Environ Microbiol 75:6696-705. 2009
    Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E...
  97. ncbi The quantification of gluconeogenesis in healthy men by (2)H2O and [2-(13)C]glycerol yields different results: rates of gluconeogenesis in healthy men measured with (2)H2O are higher than those measured with [2-(13)C]glycerol
    M T Ackermans
    Department of Clinical Chemistry, Laboratory of Endocrinology and Radiochemistry, Academic Medical Center, University of Amsterdam, The Netherlands
    J Clin Endocrinol Metab 86:2220-6. 2001
    The quantification of gluconeogenesis (GNG) by (2)H2O and [2-(13)C]glycerol and the mass isotopomer dilution analysis of glucose does not involve assumptions regarding the enrichment of the oxaloacetate precursor pool...
  98. pmc Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation
    E Albers
    Department of Chemical Reaction Engineering, Chalmers University of Technology, Goteborg, Sweden
    Appl Environ Microbiol 62:3187-95. 1996
    To prevent the loss of raw material in ethanol production by anaerobic yeast cultures, glycerol formation has to be reduced...
  99. ncbi Baseline levels of glucose metabolites, glutamate and glycerol in malignant glioma assessed by stereotactic microdialysis
    Michael Roslin
    Department of Neurosurgery, Umea University Hospital, Umea, Sweden
    J Neurooncol 61:151-60. 2003
    ..samples were collected the next day and subsequently analysed for glucose, lactate, pyruvate, glutamate and glycerol. The main objective was to establish base-line levels of the studied substances...
  100. pmc Energetics of glycerol conduction through aquaglyceroporin GlpF
    Morten Ø Jensen
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 99:6731-6. 2002
    Aquaglyceroporin GlpF selectively conducts water and linear polyalcohols, such as glycerol, across the inner membrane of Escherichia coli...
  101. pmc The activity of yeast Hog1 MAPK is required during endoplasmic reticulum stress induced by tunicamycin exposure
    Francisco Torres-Quiroz
    Department of Biotechnology, Instituto de Agroquimica y Tecnologia de los Alimentos, Consejo Superior de Investigaciones Cientificas, E 46100 Burjassot, Valencia, Spain
    J Biol Chem 285:20088-96. 2010
    ..Here, we show that the yeast high osmolarity glycerol (HOG) pathway plays a role in ER stress resistance...

Research Grants68

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  3. Pyruvate Dehydrogenase kinase as Therapeutic Target for Hepatic Steatosis, Obesit
    Robert A Harris; Fiscal Year: 2012
    ..Fatty acids synthesized in the liver in the absorptive state should be esterified to glycerol and shipped to the adipose tissue for storage, never retained in the liver...
  4. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
    ..formation under different cooling conditions and for different concentrations of cryoprotectant agents, such as glycerol. Currently available machines are macroscopic in nature and do not allow such fine measurement resolution...
  5. Atypical PKC Knockout Models: Effect on Glucose and Lipid Homeostasis
    Robert V Farese; Fiscal Year: 2013
    ..We postulate that diminished glucose transport in Alambda -KO adipocytes limits glycerol-PO4 availability for fat synthesis, and resulting leanness, possibly via hypoleptinemia, downregulates hepatic ..
  6. Mechanisms and Consequences of Intermittent Hypoxia-Induced Lipolysis
    Jonathan C Jun; Fiscal Year: 2013
    ..Mice exhibit rapid increases in FFA and glycerol levels during IH...
  7. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    Elton T Young; Fiscal Year: 2013
    ..metabolism is induced that allows them to utilize a variety of secondary carbon sources, ranging from ethanol and glycerol to complex stored carbohydrates and lipids...
  8. Novel Small Molecule Therapeutics to Treat Obesity
    SUSAN MEDGHALCHI; Fiscal Year: 2010
    ..GPAT (glycerol-3- phosphate acyltransferase) is the initial committed step in acylglycerol synthesis and has garnered interest ..
  9. Cellular Mechanisms for Increased Gluconeogenesis in Type 2 Diabetes
    Varman T Samuel; Fiscal Year: 2013
    ..g. glycerol and glutamine) and unbiased gene expression profiling...
  10. Chondroitin Sulfate Proteoglycan and Myelination in Intraventricular Hemorrhage
    Praveen Ballabh; Fiscal Year: 2010
    ..Our approach is to use a rabbit model that induces IVH with intraperitoneal glycerol. This model results in pups with hemorrhage to exhibit inflammation, cell death and subsequently, lesser ..
  11. Biogenesis of lipoarabinomannan in mycobacteria
    Mary Jackson; Fiscal Year: 2013
    ..the global family of phosphatidylinositol mannosides that carries one to four fatty acids (attached to the glycerol, inositol and/or mannose) and one to six mannose residues...
  12. Obesity related pancreatic fat worsens local injury via unsaturated fatty acids
    VIJAY PREM SINGH; Fiscal Year: 2013
    ..This was accompanied by a large increase in fatty acids and glycerol in the medium, with levels equivalent to those in aspirates from severe pancreatic necrosis in humans...
  13. Novel endonuclease-targeted approaches to nephroprotection
    Alexei G Basnakian; Fiscal Year: 2013
    ..We plan to use complimentary in vivo (glycerol-induced rhabdomyolysis) and in vitro (hemin or myoglobin) approaches to induce tubular epithelial cells injury...
  14. Analysis of the Spatiotemporal Regulation of Lipolysis
    Lisa M DiPilato; Fiscal Year: 2012
    ..to determine the functional role of these complexes at lipid droplets in the context of lipolysis by measuring glycerol release and PKA activity upon genetic knockdown of caveolin and AKAPs using RNAi approach, as well as upon ..
  15. Endocannabinoid Discrimination
    Jenny L Wiley; Fiscal Year: 2013
    ..receptors, their signaling pathways, two predominant endogenous ligands [anandamide (AEA) and 2-arachidonyl glycerol (2-AG)], and their synthetic and metabolic pathways...
  16. Glycerolipid synthesis in liver
    ROSALIND ANNE COLEMAN; Fiscal Year: 2013
    ..The pathway of TAG and phospholipid biosynthesis is initiated by the rate-limiting enzyme, glycerol-3-phosphate acyltransferase (GPAT)...
  17. MOLECULAR GENETICS OF THERMOGENESIS
    MARTIN ELLIOT YOUNG; Fiscal Year: 2013
    ..In addition, a second gene, Gdm, which encodes the mitochondrial glycerol 3-phosphate dehydrogenase and has many of the same phenotypes and properties as SLC25A25, including its location ..
  18. Cannabinoid Mechanisms in Human Gastrointestinal Motor and Sensory Functions
    MICHAEL L CAMILLERI; Fiscal Year: 2010
    ..There are endogenous cannabinoids (or endocannabinoids) such as anandamide and 2-arachidonyl glycerol. Anandamide is inactivated by a fatty acid amide hydrolase (FAAH) in vivo...
  19. Adipogenic Toxicity Study of Obesogenic Drugs
    Yuanxiang Zhao; Fiscal Year: 2013
    ..or continuous accumulation of fat in adipocytes, whereas loss of fat results from the breakdown of fat into glycerol and fatty acids in adipocytes. Weight gain could result from accumulation of fat or decrease in fat reduction...
  20. Nano-sized Cell Guidance System for Ischemic Tissue Repair
    Hyunjoon Kong; Fiscal Year: 2010
    ..We hypothesize that a hyper-branched poly(glycerol) linked with both epitopes binding with transplanted cells and those binding with vascular cell adhesion ..
  21. Transdermal delivery of 2-Arachidonoyl glycerol (2-AG) for the treatment of arthr
    Audra L Stinchcomb; Fiscal Year: 2012
    ....
  22. Determining the Function of PNPLA3 Utilizing Metabolomics and Stable Isotope Labe
    MATTHEW ALVIN MITSCHE; Fiscal Year: 2013
    ..Alternative stable isotope labeling strategies, such as 13C-glycerol or 13C-fatty acid incorporation, will also be employed to compliment the D2O incorporation data...
  23. Regulation and Function of MGAT in Hepatic TAG Synthesis and Metabolic Disease
    ANGELA MARIE HALL; Fiscal Year: 2013
    ..Lipin 1 encodes a key enzyme in the glycerol 3-phosphate (G3P) triglyceride (TAG) synthesis pathway and therefore the observed hepatic TAG accumulation is ..
  24. Reduction in CVD risk in children by physical activity
    Robert C Hickner; Fiscal Year: 2010
    ..We propose to implement the novel use of microdialysis to assess interstitial glucose and glycerol concentrations as indices of blood glucose and adipose tissue lipolysis, respectively, and adipose tissue blood ..
  25. Endocannabinoid modulation of pain-depressed behavior
    LAURA J SIMSELLEY; Fiscal Year: 2013
    ..receptors, their signaling pathways, two predominant endogenous ligands [anandamide (AEA) and 2-arachidonyl glycerol (2-AG)], and their synthetic and metabolic pathways...
  26. Inhibition of ATGL-Mediated Lipolysis by G0S2
    Jun Liu; Fiscal Year: 2013
    ..Lipolysis, the process in which TAG is hydrolyzed to FFAs and glycerol, has been a subject of intense investigation for decades, with much focus on hormone-sensitive lipase (HSL)...
  27. Regulation of renal and bone marrow injury by extracellular vesicle non-coding RN
    Peter J Quesenberry; Fiscal Year: 2013
    ..optimum approach for separating and characterizing MSC-dv carrying "healing" microRNA to renal tissue injured by glycerol exposure or by ischemia/reperfusion or marrow tissue (stem cells) injured by irradiation...
  28. Cell Wall Integrity Signaling in Yeast
    David E Levin; Fiscal Year: 2013
    ..This pathway culminates in the reduction of turgor pressure by release of intracellular glycerol through the Fps1 glycerol channel...
  29. FUNCTION AND REGULATION OF GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 2
    MARIA R GONZALEZ BARO; Fiscal Year: 2012
    ..The first and committed step in glycerolipid synthesis is the acylation of glycerol-3-phosphate, catalyzed by the enzyme glycerol-3-phosphate acyltransferase (GPAT)...
  30. Targeting the lipid synthesis enzyme, DGAT1, for breast cancer prevention
    IVAN PETER URAY; Fiscal Year: 2013
    ..diacylglcyerol acyl-transferase (DGAT1) enzyme catalyzes the formation of triglycerides by esterifying diacyl-glycerol with activated fatty acids...
  31. The Role of MTP in Lipid Droplet Formation in Adipocytes
    Larry L Swift; Fiscal Year: 2013
    ..effects of MTP deficiency on differentiation, lipid droplet formation and maturation, de novo lipogenesis, and glycerol metabolism...
  32. Activation and Inhibition of Aldehyde Dehydrogenase 2
    Thomas D Hurley; Fiscal Year: 2013
    ..Nitroglycerin or glycerol trinitrate (GTN) is a potent vasodilator due to its ability to promote the relaxation of smooth muscle cells in ..
  33. Pope-Presynaptic modulation of anticholinesterase toxicity
    Carey N Pope; Fiscal Year: 2013
    ..Endocannabinoids (eCBs, e.g., anandamide, 2-arachidonyl glycerol, 2-AG) modulate neurotransmission by activating presynaptic cannabinoid receptors and inhibiting neurotransmitter ..
  34. Effects of Dietary Omega-3 Fatty Acids on Akt Signaling in Prostate Cancer
    Yong Q Chen; Fiscal Year: 2013
    ..In mammals, the first carbon (sn-1 position) of the glycerol backbone in phosphatidylinositols (PIP) is usually linked to a saturated fatty acid, the second carbon (sn-2 ..
  35. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2011
    ..From these studies, we have found that in 1 to 1.5 M EG or glycerol, nucleation occurs near -400C, the homogeneous nucleation temperature of water...
  36. Scalable Units for Building Vascularized Cardiac Graft
    Lisa E Freed; Fiscal Year: 2013
    ..In Aim 1, we will create elastomeric scaffolds from poly (glycerol-sebacate) and elastin, and test the hypothesis that scaffold structural features (i.e...
  37. Device for Hyperfast Scalable Vitrification of Germplasm in Large Volumes
    IGOR KATKOV; Fiscal Year: 2013
    ..depend on the CPA concentration, For example, hundreds of thousands of ?C/min are needed to vitrify a water-glycerol solution that is tolerable for ALL CPA species concentrations...
  38. Cell Biology of Mycobacterium tuberculosis Infection
    David G Russell; Fiscal Year: 2010
    ..This lipase will generate free fatty acids and glycerol, and release cholesterol from LDL, all excellent carbon sources for M. tuberculosis...
  39. Regulation of the Cardiac Sodium Channel by SIRTUIN1
    Barry London; Fiscal Year: 2013
    ..Brugada Syndrome has also been linked to a mutation in the gene for the glycerol-3-phosphate dehydrogenase-like (GPD1-L) protein, which is dependent on the balance of cellular NADH and NAD+, and ..
  40. Novel Pattern Specific Isotopic Labeling of Aromatic Amino Acids
    Robert McFeeters; Fiscal Year: 2010
    ..A bacterial system has been modified to make and secrete elevated amounts of phenylalanine. Using 2-13C glycerol as the sole carbon source, C?, C?, and C? labeled phenylalanine has been made with >98% 13C incorporation...
  41. Borrelia burgdorferi tick phase genes and Lyme disease pathogenesis
    IRA S SCHWARTZ; Fiscal Year: 2013
    ..profile during spirochete colonization of the vector;2) the Glp operon that encodes proteins required for glycerol uptake and utilization, a process likely to be particularly important during B...
  42. CaMKII, endocannabinoids, synaptic plasticity and motor function
    Roger J Colbran; Fiscal Year: 2013
    ..DGL?), the rate-limiting enzyme for Ca2+-dependent synthesis of the most abundant brain eCB, 2-arachidonyl glycerol (2-AG). In addition, baseline hyperactivity of T286A-KI mice can be rescued by inhibiting 2-AG breakdown...
  43. Imaging for Y90 Microscphere SIRT Planning
    Anthony J McGoron; Fiscal Year: 2012
    ..More recently, a new synthetic biodegradable elastomer Poly(glycerol Dodecanedioate) (PGD) has been developed for medical devices and tissue engineering scaffolds...
  44. Yeast MAPK Signaling Networks: Specificity, Regulation and Function
    Jeremy W Thorner; Fiscal Year: 2013
    ..Also well studied are the high-osmolarity glycerol (HOG) pathway for coping with hyperosmotic stress (Hog1 MAPK), the filamentous growth response triggered by ..
  45. Metabolic changes in ovarian cancer cells initiated by metastasis to adipose tiss
    Ernst Lengyel; Fiscal Year: 2013
    ..experiments will be performed to determine how specific adipocyte derived Fas including oxidized lipids, and glycerol regulate lipid and glucose metabolism in OvCa cells...
  46. Nanocells for vascular normalization therapies
    Hyunjoon Kong; Fiscal Year: 2013
    ..independently tuned by (1) integrating into the nanocell via self- assembly process, a targeting module, a poly (glycerol) substituted with varying numbers of alkyl chains and leaky endothelium-targeting oligopeptides, and (2) further ..
  47. Core-Shell Fiber Mesh for the Treatment of UI and PFD in Elderly Women
    MICHAEL P H LAU; Fiscal Year: 2011
    ..The fiber core and shell are composed of poly(glycerol sebacate) (PGS) and poly(1,3-Bis- (carboxyphenoxy)propane) (PCPP), respectively selected to tunably surface erode ..
  48. Cannabinergic Ligands and Drugs
    Alexandros Makriyannis; Fiscal Year: 2012
    ..2) Novel 2-arachidonoyl glycerol (2AG) and arachidonoylethanolamine (AEA) analogs, with well-defined conformations...
  49. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2013
    ..From these studies, we have found that in 1 to 1.5 M EG or glycerol, nucleation occurs near -400C, the homogeneous nucleation temperature of water...
  50. Novel Polymeric nanoparticles for drug delivery applications
    Anthony J McGoron; Fiscal Year: 2013
    ..Recently, a synthetic biodegradable elastomer Poly(glycerol Dodecanedioate) (PGD) has been developed for medical devices and tissue engineering...
  51. Modulation of NF-kB Signaling by Immunoprobiotics
    James Versalovic; Fiscal Year: 2011
    The overall hypothesis of this proposal is that glycerol/glycerophospholipid metabolism is a focal point for determining the relative capacities of probiotics to suppress NF-?B and MAP kinase signaling in mammalian cells...
  52. Imaging endocannabinoid degradation in the mouse brain
    Sherrye T Glaser; Fiscal Year: 2010
    ..THC mimics the action of the endocannabinoids anandamide (AEA) and 2- arachidonyl glycerol (2-AG)...
  53. METABOLIC REGULATION OF INSULIN SECRETION
    Orian S Shirihai; Fiscal Year: 2013
    ..What are the mechanisms of increased basal insulin secretion by oleate, mono-oleoyl-glycerol and ROS? 2. What are the protective mechanisms that counteract basal hypersecretion and how do they fail? 3...
  54. NMR and NIR detection of phospholipase activity in vivo
    Edward J Delikatny; Fiscal Year: 2012
    ..The fluorophore will be attached to different positions of the glycerol backbone to interrogate the activity of different phospholipase isoforms...
  55. Alcohol dependence and brain endocannabinoid function
    Loren H Parsons; Fiscal Year: 2013
    ..have gathered evidence that EtOH consumption increases levels of the endogenous cannabinoid (eCB) 2- arachidonoyl glycerol (2-AG) in rodent brain, while long-term intermittent EtOH exposure down-regulates eCB signaling in brain regions ..
  56. PHOSPHOLIPID OXIDATION AND THE SCAVENGER RECEPTOR
    Stanley L Hazen; Fiscal Year: 2011
    ..We recently identified a novel family of oxidized choline glycerol-phospholipids that serve as high affinity ligands for CD36 (oxPCCD36)...
  57. CA IN MYELIN PRODUCING CELLS
    Ken McCarthy; Fiscal Year: 1993
    ..Developmental markers to be used for oligodendroglia are glycerol phosphate dehydrogenase (GPDH), galactocerebroside (GC) and myelin basic protein (MBP)...
  58. Method and Device for Rodent Germplasm Vitrification
    John K Critser; Fiscal Year: 2010
    ..g. glycerol and DMSO), or with relatively low concentrations (<1M) of CPAs, is suitable for cryopreservation of ..
  59. Cannabimimetic Ligands and Endocannabinoid Active Sites
    MAKRIYANNIS ALEXANDROS; Fiscal Year: 2011
    ..endocannabinoid deactivation including the two hydrolytic enzymes, Fatty Acid Amide Hytlrolase (FAAH) and Monacyl Glycerol Ligase (MGL), the oxidative enzyme Cyclooxygenase-2 (COX-2), and the Anandamide Transport system (ANT)...
  60. MECHANISMS OF WATER TRANSPORT IN RENAL EPITHELIA
    Alan S Verkman; Fiscal Year: 2013
    ..The hypothesis will be tested that AQP3-faciliated glycerol transport alters cellular glycerol metabolism, which is a key determinant of cellular ATP/energetics, MAPK ..
  61. MOLECULAR BASIS OF CANNABINOID ACTIVITY
    Alexandros Makriyannis; Fiscal Year: 2013
    ..We now propose to study the three key brain esterases involved in endocannabinoid deactivation, namely, monoacyl glycerol lipase (MGL), fatty acid amide hydrolase (FAAH) and [unreadable]/[unreadable]-hydrolase domain-containing 6 (..
  62. INTERACTIONS OF TRPV1 AND TRPA1 AND INFLAMMATORY PAIN
    Armen N Akopian; Fiscal Year: 2010
    ..Specific Aim #3: Determine whether inflammation-induced diacylglycerol and 2-arachydonic glycerol responses mediated by TRPA1 and controlled by TRPV1 in sensory neurons This conceptually innovative hypothesis ..
  63. Development of Nanoscale Plasmonic Devices for Creation of a Next Generation Surf
    David H Waldeck; Fiscal Year: 2012
    ..We will demonstrate the capabilities of this new technology by investigating aspects of glycerol metabolism and the specific protein-protein interactions that are essential in its regulation...
  64. Inhibition of Endocannabinoid Biosynthesis via Diacylglycerol Lipase
    RICHARD DUCLOS; Fiscal Year: 2009
    ..involves a systematic synthesis of DAGL inhibitors structurally derived from DAGL substrate (1,2-diacyl-sn-glycerol) and the selective inhibitor tetrahydrolipstatin (THL), which has poor solubility and absorption characteristics...
  65. ALTERNATIVE ROLES FOR PHOSPHOENOLPYRUVATE CARBOXYKINASE
    Paul Ray; Fiscal Year: 1991
    ..C PEPCK supports glycerogenesis in adipose tissue although glycerol formation from its most common source, glucose, does not require PEPCK...
  66. FREEZING STORAGE OF HEART AT HIGH SUBZERO TEMPERATURES
    Tingchung Wang; Fiscal Year: 1993
    ..high subzero temperatures (-8 degrees C or higher) employing modest levels of natural cryoprotectants (glucose, glycerol, sorbitol, etc.)...
  67. ENZYMES IN PHOSPHATIDIC ACID BIOSYNTHESIS
    Hei Sook Sul; Fiscal Year: 2002
    ..serves as the backbone of glycerophospholipid biosynthesis, is synthesized de novo by the sequential acylation of glycerol-3-phosphate...
  68. INTERMEDIARY METABOLISM OF LEISHMANIA
    JACOB BLUM; Fiscal Year: 1993
    ..as sole carbon source in the presence of air or oxygen release acetate, pyruvate, succinate, and small amounts of glycerol into the buffered salt solution...