Genomes and Genes
Summary: A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols.
Publications244 found, 100 shown here
- Redox regulation of the DNA repair function of the human AP endonuclease Ape1/ref-1M R Kelley
Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA
Antioxid Redox Signal 3:671-83. 2001..These findings suggest that the Apel/ref-1 protein may be much more intimately regulated at the posttranslational level than initially imagined...
- High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitolShixuan Wu
U S Department of Agriculture, Laramie, WY, USA
Biotechniques 36:152-4. 2004..We report here that pretreatment of P. pastoris with 0.1 M lithium acetate (LiAc) and 10 mM dithiothreitol (DTT) before electroporation increased transformation efficiency approximately 150-fold...
- Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitolKeith Vosseller
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
Proteomics 5:388-98. 2005..The method (BEMAD) involves differential isotopic labeling through Michael addition with normal dithiothreitol (DTT) (d0) or deuterated DTT (d6), and enrichment of these peptides by thiol chromatography...
- Vitamin K epoxide reductase contributes to protein disulfide formation and redox homeostasis within the endoplasmic reticulumLori A Rutkevich
Department of Biochemistry, University of Toronto, Toronto, ON, Canada
Mol Biol Cell 23:2017-27. 2012..In contrast, no involvement of QSOX1 in ER oxidative processes could be detected. These findings establish VKOR as a significant contributor to disulfide bond formation within the ER...
- Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress responseHiromi Tajima
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
Biochem Biophys Res Commun 374:242-7. 2008..conditions, GFP fluorescence localization almost overlapped with an ER marker; however, tunicamycin and dithiothreitol treatment clearly increased GFP fluorescence in the nucleus suggesting that the N-terminal fragment of ..
- Structural perturbation and enhancement of the chaperone-like activity of alpha-crystallin by arginine hydrochlorideVolety Srinivas
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
Protein Sci 12:1262-70. 2003..This study should prove useful in designing strategies to improve chaperone function for therapeutic applications...
- Reversal by dithiothreitol treatment of the block in murine leukemia virus maturation induced by disulfide cross-linkingStephen Campbell
HIV Drug Resistance Program, SAIC Frederick, National Cancer Institute Frederick, Natipnal Institutes of Health, Frederick, Maryland 21702, USA
J Virol 76:10050-5. 2002..We now show that treatment of these immature particles with a reducing agent (dithiothreitol) induces their maturation in vitro, as evidenced by proteolytic cleavage of Gag, Gag-Pol, and Env proteins and ..
- Effects of oocyte activation and sperm preparation on the development of porcine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm injectionJian Hui Tian
Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, PR China
Theriogenology 66:439-48. 2006..freeze-thaw cycles (without cryoprotectant) on oocyte activation and the effects of sperm pre-incubated with dithiothreitol (DTT) or Triton X-100 on early embryo development were also examined...
- Is dithiothreitol affecting cells and soluble mediators during sputum processing? A modified methodology to process sputumPatrizia Pignatti
J Allergy Clin Immunol 110:667-8. 2002
- The secretory pathway is normal in dithiothreitol-treated cells, but disulfide-bonded proteins are reduced and reversibly retained in the endoplasmic reticulumH F Lodish
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
J Biol Chem 268:20598-605. 1993Addition of 2 mM dithiothreitol (DTT) to HepG2 human hepatoma cells blocks secretion of newly made albumin and causes it to accumulate in the endoplasmic reticulum in the reduced state...
- Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivityUtpal B Pajvani
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Biol Chem 278:9073-85. 2003..Surprisingly, Acrp30(C39S) or wild-type Acrp30 treated with dithiothreitol are significantly more bioactive than the higher order oligomeric forms of the protein with respect to ..
- Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulumM G Pollard
Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143 0450, USA
Mol Cell 1:171-82. 1998..We conclude that there exists a specific cellular redox machinery required for disulfide-linked protein folding in the ER and that Ero1p is an essential component of this machinery...
- Dithiothreitol abrogates the effect of arsenic trioxide on normal rat liver mitochondria and human hepatocellular carcinoma cellsManash K Paul
Department of Biology, Indian Institute of Science Education and Research, Mohali, MGSIPA Complex, Adjacent Sacred Heart School, Sector 26, Chandigarh 160019 corrected India
Toxicol Appl Pharmacol 226:140-52. 2008..b>Dithiothreitol (DTT) is a well-known dithiol agent reported to modulate the action of ATO...
- Cysteine-25 of adenylate kinase reacts with dithiothreitol to form an adduct upon aging of the enzymeX Li
National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, 15 Datun Road, Beijing 100101, PR China
FEBS Lett 507:169-73. 2001Adenylate kinase (AK) ages in solution in the presence of DL-dithiothreitol (DTT) with a gradual activity decrease...
- High efficiency transformation of Schizosaccharomyces pombe pretreated with thiol compounds by electroporationM Suga
Department of Materials and Biosystem Engineering, Toyama University, 3190 Gofuku, Toyama City, Toyama 930 8555, Japan
Yeast 18:1015-21. 2001..Among the thiol compounds tested, dithiothreitol (DTT) was the most effective for pretreatment...
- Bovine embryo development following ICSI: effect of activation, sperm capacitation and pre-treatment with dithiothreitolCesare Galli
Laboratorio di Tecnologie della Riproduzione, CIZ srl, Istituto Sperimentale Italiano Lazzaro Spallanzani, Via Porcellasco 7 f, 26100 Cremona, Italy
Theriogenology 60:1467-80. 2003..Finally, we studied the effect of dithiothreitol (DTT) pre-treatment of sperm or of injected oocytes...
- Reversible inhibitors of lambda integrase-mediated recombination efficiently trap Holliday junction intermediates and form the basis of a novel assay for junction resolutionJeffrey L Boldt
Department of Biology and Center for Microbial Sciences, San Diego State University, San Diego, California 92182 4614, USA
J Biol Chem 279:3472-83. 2004..This assay is particularly useful for determining junction resolution rates in cases where complexes directly assembled on junction substrates undergo little or no catalysis...
- A new cell culture-based assay quantifies vitamin K 2,3-epoxide reductase complex subunit 1 function and reveals warfarin resistance phenotypes not shown by the dithiothreitol-driven VKOR assayA Fregin
Institute of Human Genetics, University of Wurzburg, Wurzburg, Germany
J Thromb Haemost 11:872-80. 2013..VKORC1 function has historically been assessed in vitro using a dithiothreitol (DTT)-driven vitamin K 2,3-epoxide reductase (VKOR) assay...
- Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII inteinKenneth V Mills
College of the Holy Cross, Department of Chemistry, Worcester, Massachusetts 01610, USA
J Biol Chem 280:2714-20. 2005..The pseudo first order rate constant of dithiothreitol-dependent N-terminal cleavage is 1 x 10(-4) s(-1). The first order rate constant of C-terminal cleavage is 1...
- Sperm movement in the ooplasm, dithiothreitol pretreatment and sperm freezing are not required for the development of porcine embryos derived from injection of head membrane-damaged spermHwan Yul Yong
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
Theriogenology 63:783-94. 2005The present study investigated the correlation of sperm movement in the ooplasm, pretreatment of sperm with dithiothreitol (DTT) and sperm freezing with the development of porcine embryos derived from modified intracytoplasmic sperm ..
- Effects of dithiothreitol and boar on pronuclear formation and embryonic development following intracytoplasmic sperm injection in pigsHiroyuki Watanabe
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 8555, Japan
Theriogenology 65:528-39. 2006..fertilization, male pronuclear formation and embryonic development following intracytoplasmic injection of dithiothreitol (DTT)-treated boar spermatozoa...
- UPR-independent dithiothreitol stress-induced genes in Aspergillus nigerD A MacKenzie
Department of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
Mol Genet Genomics 274:410-8. 2005A subtraction library was prepared from cultures of Aspergillus niger that had or had not been exposed to dithiothreitol (DTT), in order to identify genes involved in the unfolded protein response (UPR) or in the response to reductive ..
- Selective retention of secretory proteins in the yeast endoplasmic reticulum by treatment of cells with a reducing agentE Jämsä
Institute of Biotechnology, University of Helsinki, Finland
Yeast 10:355-70. 1994..Treatment of living cells with the reducing agent dithiothreitol (DTT) prevented disulfide formation of newly synthesized CPY and hsp150, resulting in retention of the ..
- Ascorbic and dehydroascorbic acids measured in plasma preserved with dithiothreitol or metaphosphoric acidS A Margolis
Organic Analytical Chemistry Division, National Institute for Standards and Technology, Gaithersburg, MD 20899
Clin Chem 36:1750-5. 1990..83 mg/L. The stability and homogeneity of lyophilized plasma samples supplemented with ascorbic acid and dithiothreitol are documented...
- Mechanism of suppression of dithiothreitol-induced aggregation of bovine alpha-lactalbumin by alpha-crystallinZoya M Bumagina
A N Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia
Biophys Chem 146:108-17. 2010The kinetics of dithiothreitol (DTT)-induced aggregation of alpha-lactalbumin from bovine milk has been studied using dynamic light-scattering technique...
- Different activation treatments for successful development of bovine oocytes following intracytoplasmic sperm injectionS A Ock
Gyeongsang National University, Republic of Korea and Madras Veterinary College, Tamil Nadu, India
Zygote 11:69-76. 2003..Motile spermatozoa selected by Percoll density gradient were treated with 5 mM dithiothreitol (DTT) and analysed for ultrastructural changes of the head using transmission electron microscopy (TEM)...
- Quantifying dithiothreitol displacement of functional ligands from gold nanoparticlesDe Hao Tsai
Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Anal Bioanal Chem 404:3015-23. 2012b>Dithiothreitol (DTT)-based displacement is widely utilized for separating ligands from their gold nanoparticle (AuNP) conjugates, a critical step for differentiating and quantifying surface-bound functional ligands and therefore the ..
- Self-arrangement among charge-stabilized gold nanoparticles on a dithiothreitol reactivated octanedithiol monolayerAnders O Lundgren
Department of Cell and Molecular Biology, Interface Biophysics, Goteborg University, SE 405 30 Goteborg, Sweden
Nano Lett 8:3989-92. 2008Gold surfaces and structures modified with octanedithiol were reacted with dithiothreitol prior to immersion in buffered solutions of charge stabilized gold nanoparticles...
- Dependence of product formation from decomposition of nitroso-dithiols on the degree of nitrosation. Evidence that dinitroso-dithiothreitol acts solely as an nitric oxide releasing compoundSonja Liebeskind
Institut fur Physiologische Chemie, Universitatsklinikum Essen, Hufelandstrasse 55, 45122, Essen, Germany
Org Biomol Chem 6:2560-73. 2008..In this paper, we demonstrate that nitrosated dl-dithiothreitol (DTT) decays via two independent pathways, that is, one producing exclusively nitric oxide and one producing (..
- Significant increases in potato Starch-Synthase and Starch-Branching-Enzyme activities by dilution with buffer containing dithiothreitol and polyvinyl alcohol 50 KRupendra Mukerjea
Laboratory of Carbohydrate Chemistry and Enzymology, The Roy J Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
Carbohydr Res 367:25-8. 2013..5), containing 1.0mM dithiothreitol (DTT) and 0...
- Substitution of serine for proline in the active center of type 2 iodothyronine deiodinase substantially alters its in vitro biochemical properties with dithiothreitol but not its function in intact cellsIuri Martin Goemann
Thyroid Section, Endocrine Division, Hospital de Clinicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035 903, Brazil
Endocrinology 151:821-9. 2010..show that despite an approximately 2000-fold higher Michaelis constant (K(m); T(4)) for D1 than for D2 using dithiothreitol (DTT) as cofactor, D1 expressed in intact cells produces T(3) at free T(4) concentrations many orders of ..
- Synergistic cytotoxic effects of arsenic trioxide plus dithiothreitol on mice oral cancer cellsChia Wen Tsai
Terry Fox Cancer Research Lab, Graduate Institute of Chinese Medical Science, School of Medicine, China Medical University, Taichung, 404 Taiwan, ROC
Anticancer Res 30:3655-60. 2010..The results showed that 10-20 μM dithiothreitol enhanced the cytotoxic effects of arsenic trioxide to 43.3~62...
- Reducing agent dithiothreitol facilitates activity of the capsaicin receptor VR-1L Vyklicky
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Neuroscience 111:435-41. 2002..On the extracellular side, VR1 has three cysteine residues at positions 616, 621 and 634. Here we report that dithiothreitol (DTT) (2-60 mM), an agent that maintains -SH groups of cysteines in a reduced state, greatly facilitates ..
- Redox/pH dual stimuli-responsive biodegradable nanohydrogels with varying responses to dithiothreitol and glutathione for controlled drug releaseYuan Jia Pan
State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China
Biomaterials 33:6570-9. 2012..1) in the presence of 10 mM dithiothreitol (DTT) or glutathione (GSH)...
- Modification of actions of heat shock on development and apoptosis of cultured preimplantation bovine embryos by oxygen concentration and dithiothreitolL A de Castro e Paula
Department of Animal Sciences, University of Florida, Gainesville, Florida, USA
Mol Reprod Dev 75:1338-50. 2008..low oxygen environment (5% oxygen) and (2) that these effects would be reversed by addition of the antioxidant dithiothreitol (DTT)...
- Direct evidence that two cysteines in the dopamine transporter form a disulfide bondRong Chen
Department of Pharmacology, The Ohio State University College of Medicine, 333 West 10th Avenue, Columbus, OH 43210, USA
Mol Cell Biochem 298:41-8. 2007..Our results and previous results are consistent with the notion that the disulfide bond between EL2 cysteines is required for DAT biosynthesis and/or its delivery to the cell surface...
- Beneficial effects of dithiothreitol on relative levels of glutathione S-transferase activity and thiols in oocytes, and cell number, DNA fragmentation and allocation at the blastocyst stage in the mouseFrancisco Rausell
Department of Functional Biology and Physical Anthropology, Faculty of Biological Sciences, University of Valencia, Valencia, Spain
Mol Reprod Dev 74:860-9. 2007We analyzed the effect of in vitro aging of mouse oocytes in the presence of dithiothreitol (DTT) on relative levels of glutathione S-transferase (GST) activity and thiols in oocytes, and cell number, DNA fragmentation and cellular ..
- Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803D D Martin
New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
Biochemistry 40:1393-402. 2001..Observed rate constants (k(obs)) for dithiothreitol-induced N-terminal cleavage, C-terminal cleavage, and trans-splicing were (1.0 +/- 0.5) x 10(-3), (1.9 +/- 0...
- Additive effects of bovine serum albumin, dithiothreitol, and glycerol on PCRM Nagai
Department of Biochemistry, Iwate Medical University School of Dentistry, Japan
Biochem Mol Biol Int 44:157-63. 1998The effects of bovine serum albumin, dithiothreitol, and glycerol on PCR were studied. PCR under standard conditions failed the amplification of an enterohemorrhagic E...
- A sulfhydryl oxidase from chicken egg whiteK L Hoober
Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
J Biol Chem 271:30510-6. 1996..b>Dithiothreitol was the best substrate of those tested, with a turnover number of 1030/min, a Km of 150 microM, and a pH ..
- Arginine hydrochloride enhances the dynamics of subunit assembly and the chaperone-like activity of alpha-crystallinV Srinivas
Centre for Cellular and Molecular Biology, Hyderabad, India
Mol Vis 11:249-55. 2005..The purpose of the present study is to get an insight into the mechanism of the arginine induced enhancement of chaperone-like activity of alpha-crystallin...
- Insights into hydrophobicity and the chaperone-like function of alphaA- and alphaB-crystallins: an isothermal titration calorimetric studyM Satish Kumar
Biochemistry Division, National Institute of Nutrition, Hyderabad 500 007, India
J Biol Chem 280:21726-30. 2005..Our results indicate that although hydrophobicity appears to be a factor in determining the chaperone-like activity of alpha-crystallins, it does not quantitatively correlate with the chaperone function of alpha-crystallins...
- The activity-induced reduction of myofibrillar Ca2+ sensitivity in mouse skeletal muscle is reversed by dithiothreitolTerence R Moopanar
Institute for Biomedical Sciences, School of Medical Sciences, University of Sydney F13, NSW 2006, Australia
J Physiol 571:191-200. 2006..ii) If the membrane-permeant thiol-specific reducing agent dithiothreitol (0...
- Interaction of metals and thiols in cell damage and glutathione distribution: potentiation of mercury toxicity by dithiothreitolB Hultberg
Department of Clinical Chemistry, Institute of Laboratory Medicine, University Hospital, S-22185 Lund, Sweden
Toxicology 156:93-100. 2001..The addition of dithiothreitol (DTT) but not the other thiols potentiated the effects of mercury ions on glutathione distribution and cell ..
- Effect of phosphorylation on alpha B-crystallin: differences in stability, subunit exchange and chaperone activity of homo and mixed oligomers of alpha B-crystallin and its phosphorylation-mimicking mutantMd Faiz Ahmad
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
J Mol Biol 375:1040-51. 2008..We propose that the degree of phosphorylation of the alpha B-crystallin oligomers and temperature are key modulators to achieve a wide range of chaperone capabilities of the small heat shock protein, alpha -crystallin...
- Optimized dialysis and protease inhibition of sputum dithiothreitol supernatantsEdward M Erin
NHLI Clinical Studies Unit, Royal Brompton Hospital, Fulham Road, London SW3 6HP, UK
Am J Respir Crit Care Med 177:132-41. 2008b>Dithiothreitol (DTT) is commonly used to liquefy induced sputum samples before assessment of cytology, but causes reduction of disulfide bonds and denaturation of proteins.
- Purification and characterization of a new trypsin inhibitor from Dimorphandra mollis seedsG C Mello
Departamento de Bioquimica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil
J Protein Chem 20:625-32. 2001..The inhibitory activity was stable over a wide pH range and in the presence of DTT. The N-terminal sequence of DMTI-II showed a high degree of homology with other Kunitz-type inhibitors...
- Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and bronchiectasisI S Woolhouse
Department of Medicine, Queen Elizabeth Hospital, Birmingham, UK
Thorax 57:667-71. 2002..However, it is not known whether dithiothreitol (DTT), a reducing mucolytic agent regularly used to homogenise sputum, affects the detection of inflammatory ..
- Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progressionNicole Magnasco Nichols
New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
Biochemistry 42:5301-11. 2003..By inhibiting both the splicing pathway and spontaneous hydrolysis with Zn(2+), reactants can be diverted from the trans splicing to the trans cleavage pathway where DTT and EDTA can regulate N- and C-terminal cleavage, respectively...
- Role of disulfide bonds in Acrp30/adiponectin structure and signaling specificity. Different oligomers activate different signal transduction pathwaysTsu Shuen Tsao
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
J Biol Chem 278:50810-7. 2003..HMW) forms of Acrp30 depends upon formation of Cys22-mediated disulfide bonds because their reduction with dithiothreitol or substitution of Cys22 with alanine led exclusively to trimers...
- Conformational change and inactivation of arginine kinase from shrimp Feneropenaeus chinensis in oxidized dithiothreitol solutionsJi Cheng Pan
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People s Republic of China
Biochem Cell Biol 82:361-7. 2004The effect of oxidized dithiothreitol (DTT) on the conformation and function of arginine kinase from shrimp Feneropenaeus chinensis was investigated with the methods of intrinsic fluorescence, ANS fluorescence, size exclusion ..
- Caffeine and dithiothreitol delay ovine oocyte ageingXiao Fang Ye
Xinjiang Key Laboratory of Animal Biotechnology, Xinjiang Academy of Animal Sciences, Urumqi 830000, China
Reprod Fertil Dev 22:1254-61. 2010..somatic cell nuclear transfer and intracytoplasmic sperm injection in the presence or absence of caffeine or dithiothreitol (DTT) were examined...
- A bio-bar-code assay based upon dithiothreitol-induced oligonucleotide releaseC Shad Thaxton
Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
Anal Chem 77:8174-8. 2005..on the ability to liberate the adsorbed thiolated oligonucleotides from the gold nanoparticle surface with dithiothreitol (DTT), which simplifies the assay and increases its quantitative capabilities...
- The papaya Kunitz-type trypsin inhibitor is a highly stable beta-sheet glycoproteinMohamed Azarkan
University of Brussels, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme CP 609, 808, Route de Lennik, Bz 1070 Brussels, Belgium
Biochim Biophys Acta 1764:1063-72. 2006..Exposure to 2.5 mM dithiothreitol for 120 min caused a 33% loss of the inhibitory activity, while a loss of 75% was obtained in the presence of ..
- Reduction with dithiothreitol causes serglycin-specific defects in secretory granule integrity of bone marrow derived mast cellsTiago Braga
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, Uppsala, Sweden
Mol Immunol 46:422-8. 2009..Interference with such motifs of neuroendocrine cells with dithiothreitol (DTT) has previously been shown to cause cellular missorting...
- The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulumA R Frand
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Mol Cell 1:161-70. 1998..We show that glutathione is not required for CPY folding and conclude that Ero1p functions in a novel mechanism that sustains the ER oxidizing potential, supporting net formation of protein disulfide bonds...
- Dynamic rearrangement of the outer mouth of a K+ channel during gatingY Liu
Department of Neurobiology, Harvard Medical School, Massachusetts General Hospital, Boston 02114, USA
Neuron 16:859-67. 1996..These results give a consistent picture in which C-type inactivation promotes a local rearrangement and constriction of the channel at the outer mouth...
- Structure and mechanism of peptide methionine sulfoxide reductase, an "anti-oxidation" enzymeW T Lowther
Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, University of Oregon, Eugene, Oregon 97403, USA
Biochemistry 39:13307-12. 2000..The structures support a unique, thiol-disulfide exchange mechanism that relies upon an essential cysteine as a nucleophile and additional conserved residues that interact with the oxygen atom of the sulfoxide moiety...
- Isolation and characterization of a trypsin-chymotrypsin inhibitor from the seeds of green lentil (Lens culinaris)Allen H K Cheung
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Protein Pept Lett 14:859-64. 2007..The trypsin inhibitory activity of the inhibitor was sensitive to the reducing agent dithiothreitol. It was completely abrogated after treatment with 10 mM dithiothreitol for 20 minutes...
- The mode of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallinF A Bettelheim
Chemistry Department, Adelphi University, Garden City, New York, 11530, USA
Biochem Biophys Res Commun 261:292-7. 1999..The chaperoned complex (alpha-crystallin plus alpha-lactalbumin) is in dynamic equilibrium both with the monomeric and the aggregated alpha-lactalbumin population...
- Botulinum neurotoxin types A, B, and E: fragmentations by autoproteolysis and other mechanisms including by O-phenanthroline-dithiothreitol, and association of the dinucleotides NAD(+)/NADH with the heavy chain of the three neurotoxinsBibhuti R Dasgupta
Department of Food Microbiology and Toxicology, University of Wisconsin Madison, 1925 Willow Drive, Madison, WI 53706, USA
Protein J 24:337-68. 2005..The metal chelator O-phenanthroline (above critical miceller concentration) in the presence of dithiothreitol cleaved type E NT at limited sites generating discrete 114-, 87-, 49-, 42-, and 31-kDa fragments but degraded ..
- Combination of dithiothreitol and detergent treatment of spermatozoa causes paternal chromosomal damageMonika A Szczygiel
Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
Biol Reprod 67:1532-7. 2002Treatment of spermatozoa with either the nonionic detergent Triton X-100 (TX) or dithiothreitol (DTT) has been suggested to confer enhanced success on intracytoplasmic sperm injection (ICSI) in mice and humans...
- Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivoD Missiakas
Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132
Proc Natl Acad Sci U S A 90:7084-8. 1993..coli from the lethal effects of dithiothreitol (DTT), and (ii) screening of insertion libraries of E. coli for DTT-sensitive mutants...
- Dithiothreitol induces the sacrificial antioxidant property of human serum albumin in a metal-catalyzed oxidation and gamma-irradiation systemI G Kim
Department of Radiation Biology, Korea Atomic Energy Research Institute, Yusong, Taejon
Arch Biochem Biophys 388:1-6. 2001..Intact or Cys-34-modified human serum albumin (HSA) was significantly degraded in the MCO system containing dithiothreitol (DTT) as electron donor, but as long as it lasted, HSA prohibited *OH or H2O2 from initiating molecular damage ..
- High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitolMaria Papagianni
Department of Hygiene and Technology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece
BMC Biotechnol 7:15. 2007..In the present study, treatment with lithium acetate (LiAc) and dithiothreitol (DTT) in various combinations was applied to L. lactis spp...
- Suppression of DTT-induced aggregation of abrin by alphaA- and alphaB-crystallins: a model aggregation assay for alpha-crystallin chaperone activity in vitroG Bhanuprakash Reddy
Biochemistry Division, National Institute of Nutrition, Hyderabad 500 007, India
FEBS Lett 522:59-64. 2002..In this study, we demonstrate that dithiothreitol (DTT)-induced aggregation of abrin B-chain could be monitored by light scattering similar to that of insulin...
- Cancer-preventive selenocompounds induce a specific redox modification of cysteine-rich regions in Ca(2+)-dependent isoenzymes of protein kinase CR Gopalakrishna
Department of Cell and Neurobiology, School of Medicine, University of Southern California, Los Angeles 90033, USA
Arch Biochem Biophys 348:25-36. 1997....
- Kinetics of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallinFrederick A Bettelheim
Chemistry Department, Adelphi University, Garden City, NY 11530, USA
Int J Biol Macromol 30:161-9. 2002..k(4) is somewhat faster than k(3) and it is three times faster than k(s) governing the self-aggregation of lactalbumin in the absence of alpha-crystallin...
- Dithiothreitol causes HIV-1 integrase dimer dissociation while agents interacting with the integrase dimer interface promote dimer formationManuel Tsiang
Gilead Sciences, 333 Lakeside Drive, Foster City, California 94404, United States
Biochemistry 50:1567-81. 2011..Surprisingly, we found that dithiothreitol (DTT), a reducing agent essential for in vitro enzymatic activity of IN, weakened the interaction between IN ..
- Increased concentrations of radioisotopically-labeled complementary ribonucleic acid probe, dextran sulfate, and dithiothreitol in the hybridization buffer can improve results of in situ hybridization histochemistryErik Hrabovszky
Department of Biology, University of Massachusetts, Amherst, Massachusetts, USA
J Histochem Cytochem 50:1389-400. 2002..signals resulting when varying concentrations of (35)S-labeled cRNA probes, dextran sulfate (DS), and dithiothreitol (DTT) were used for ISHH...
- Paradoxical acceleration of dithiothreitol-induced aggregation of insulin in the presence of a chaperoneZoya Bumagina
A N Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia E Mails Z B N A B K
Int J Mol Sci 11:4556-79. 2010The kinetics of dithiothreitol (DTT)-induced aggregation of human recombinant insulin and the effect of α-crystallin, a representative of the family of small heat shock proteins, on the aggregation process have been studied using dynamic ..
- Lipid peroxidation-dependent and -independent protein thiol modifications in isolated rat hepatocytes: differential effects of vitamin E and disulfiramP Dogterom
Division of Toxicology, University of Leiden, The Netherlands
Chem Biol Interact 71:291-306. 1989..in cells, decreased to 50% by exposure to AA, DEM and BIU, could be reversed to 75% of the initial level by dithiothreitol (DTT) treatment, indicating that the protein thiols were partially modified into disulfides and partially into ..
- Tris(2-carboxyethyl)phosphine stabilization of RNA: comparison with dithiothreitol for use with nucleic acid and thiophosphoryl chemistrySteven S Rhee
Department of Chemistry, Indiana University, Bloomington, IN 47405 7102, USA
Anal Biochem 325:137-43. 2004..The sulfhydryls of dithiothreitol (DTT) compete with thiophosphates for binding to the mercury within [(N-acryloylamino)phenyl] mercuric ..
- Coordination of heavy metals by dithiothreitol, a commonly used thiol group protectantA Kr zel
Faculty of Chemistry, , Poland
J Inorg Biochem 84:77-88. 2001D,L-Dithiothreitol (DTT), known also as Cleland reagent, is a thiol group protectant, used commonly in peptide and protein chemistry...
- Production of intracellular superoxide mediates dithiothreitol-dependent inhibition of apoptotic cell deathMarie Veronique Clement
Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore 117 597
Antioxid Redox Signal 7:456-64. 2005..of cysteine residues in and around the catalytic site of the proteases, cysteine- reducing agents such as dithiothreitol (DTT) are expected to facilitate caspase activity upon induction of apoptosis...
- Electrocyte (Na(+),K(+))ATPase inhibition induced by zinc is reverted by dithiothreitolM G L Ribeiro
Laboratório de Físico Química Biológica, Instituto de Biofisica Carlos Chagas Filho, UFRJ CCS Ilha do Fundão, Av Brigadeiro Trompowsky, Rio de Janeiro, RJ, Brazil
Int J Biochem Cell Biol 34:516-24. 2002..electricus main electric organ homogenate. We tried to recover the enzyme activity using dithiothreitol, a reducing agent...
- Active site mutations in yeast protein disulfide isomerase cause dithiothreitol sensitivity and a reduced rate of protein folding in the endoplasmic reticulumB Holst
Department of Yeast Genetics, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK 2500 Copenhagen Valby, Denmark
J Cell Biol 138:1229-38. 1997..Exchanging the wild-type Eug1p C(L/I)HS active site sequences for C(L/I)HC increased the growth rate significantly, however, further highlighting the importance of the oxidizing function for optimal growth...
- Inactivation of the SMN complex by oxidative stressLili Wan
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
Mol Cell 31:244-54. 2008..Thus, the SMN complex is a redox-sensitive assemblyosome and an ROS target, suggesting that it may play a role in oxidative stress pathophysiology, which is associated with many degenerative diseases...
- 20 S proteasome from Saccharomyces cerevisiae is responsive to redox modifications and is S-glutathionylatedMarilene Demasi
Departmento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, 277 São Paulo, Sao Paulo 05508 900, Brazil
J Biol Chem 278:679-85. 2003..The present results indicate that at the physiological level the yeast 20 S proteasome is regulated by its sulfhydryl content, thereby coupling intracellular redox signaling to proteasome-mediated proteolysis...
- Highly conserved cysteines of mouse core 2 beta1,6-N-acetylglucosaminyltransferase I form a network of disulfide bonds and include a thiol that affects enzyme activityTen Yang Yen
Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132, USA
J Biol Chem 278:45864-81. 2003..by liquid chromatography/electrospray ionization tandem mass spectrometry in the absence and presence of dithiothreitol. Of nine highly conserved Cys residues, under both conditions, one (Cys217) is a free thiol, and eight are ..
- Single-molecule covalent chemistry with spatially separated reactantsTudor Luchian
Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843 1114, USA
Angew Chem Int Ed Engl 42:3766-71. 2003
- Familial amyotrophic lateral sclerosis mutants of copper/zinc superoxide dismutase are susceptible to disulfide reductionAshutosh Tiwari
Department of Neurology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 278:5984-92. 2003..Decreased metal binding site occupancy and exposure to the disulfide-reducing agents dithiothreitol, Tris(2-carboxyethyl)phosphine (TCEP), or reduced glutathione increased the fraction of anomalously migrating ..
- Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retinaLing Yu
Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, USA
Biochim Biophys Acta 1773:1473-82. 2007..These findings suggest that PRL-1 is a molecular component of the photoreceptor's response to oxidative stress acting upstream of the glutathione system...
- The Saccharomyces cerevisiae proteome of oxidized protein thiols: contrasted functions for the thioredoxin and glutathione pathwaysNatacha Le Moan
Laboratoire Stress Oxydants et Cancer, Service de Biologie Moléculaire Systémique, Departement de Biologie Joliot Curie, CEA Saclay, F 91191 Gif sur Yvette Cedex, France
J Biol Chem 281:10420-30. 2006..Taken together, these results suggest the high selectivity of cytoplasmic protein thiol oxidation...
- Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferaseGabino F Sanchez-Perez
Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Científicas CSIC Universidad Autónoma de Madrid, Arturo Duperier 4, 28029 Madrid, Spain
J Biol Chem 278:7285-93. 2003..The conditions of refolding, dialysis in the presence of 10 mm dithiothreitol or 10 mm GSH with 1 mm GSSG, allowed the production of both isoforms, the nature of the redox agent ..
- Juxtaposition of the two distal CX3C motifs via intrachain disulfide bonding is essential for the folding of Tim10Scott Allen
School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
J Biol Chem 278:38505-13. 2003..Taken together these data reveal this specific intramolecular disulfide bonding as the crucial mechanism for Tim10 folding and show that the inner cysteine pair has a more prominent role in this process...
- Reduction of pentavalent antimony by trypanothione and formation of a binary and ternary complex of antimony(III) and trypanothioneSiucheong Yan
Department of Chemistry and Open Laboratory of Chemical Biology, University of Hong Kong, Pokfulam Road, Hong Kong, PR China
J Biol Inorg Chem 8:689-97. 2003..Although thermodynamically stable, the complex is kinetically labile and the free and bound forms of thiolates exchange on the (1)H NMR timescale. Such a facile exchange may be crucial for the transport of Sb(III) within parasites...
- Structural and functional determinants in the S5-P region of HCN-encoded pacemaker channels revealed by cysteine-scanning substitutionsKa Wing Au
Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong
Am J Physiol Cell Physiol 294:C136-44. 2008..Interestingly, the loss of function phenotype of F339C could be rescued by the reducing agent dithiothreitol (DTT)...
- Effects of oxidation and reduction on contractile function in skeletal muscle fibres of the ratG D Lamb
Department of Zoology, La Trobe University, Melbourne, Victoria 3086, Australia
J Physiol 546:149-63. 2003..oxidants hydrogen peroxide (H(2)O(2)) and 2,2'-dithiodipyridine (DTDP), and reductants, glutathione (GSH) and dithiothreitol (DTT), on the properties of the contractile apparatus of rat fast- and slow-twitch skeletal muscle fibres, in ..
- Quantification of protein thiols and dithiols in the picomolar range using sodium borohydride and 4,4'-dithiodipyridineRosa E Hansen
Institute of Molecular Biology, University of Copenhagen, DK 2100 Copenhagen, Denmark
Anal Biochem 363:77-82. 2007..acid], DTNB) is the most widely used reagent for quantification of protein thiols, whereas dithiothreitol (DTT) is commonly used for disulfide reduction...
- Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimerValérie Noguera-Mazon
CNRS, Unite Mixte de Recherche, 5180 Sciences Analytiques, Ecole Supérieure Chimie PhysiqueElectronique de Lyon, Domaine Scientifique de la Doua, Universite Claude Bernard, Lyon1, 69622 Villeurbanne, France
J Biol Chem 281:31736-42. 2006..D-Prx induces the dissociation of the Prx non-covalent homodimer, which can be recovered by reduction with dithiothreitol. This work demonstrates for the first time the existence of a redox-dependent dimer-monomer switch in the Prx ..
- A single mutation at Tyr143 of human S-adenosylhomocysteine hydrolase renders the enzyme thermosensitive and affects the oxidation state of bound cofactor nicotinamide-adenine dinucleotideRobert Beluzić
Division of Molecular Medicine, Institute Ruder Boskovic, Bijenicka 54, 10000 Zagreb, Croatia
Biochem J 400:245-53. 2006..In summary, a single mutation in the AdoHcyase affecting both the oxidation state of bound co-factor NAD and enzyme stability is present in a human with AdoHcyase deficiency...
- Transcriptional activation of dehalorespiration. Identification of redox-active cysteines regulating dimerization and DNA bindingStelian M Pop
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
J Biol Chem 281:26382-90. 2006..When incubated with oxygen or diamide, CprK undergoes inactivation; subsequent treatment with dithiothreitol restores activity...
- The N-terminal domain of PILB from Neisseria meningitidis is a disulfide reductase that can recycle methionine sulfoxide reductasesJunzhu Wu
UMR CNRS UHP 7567, Maturation des ARN et Enzymologie Moléculaire, Faculte des Sciences, Bld des Aiguillettes, BP 239, 54506 Vandoeuvre les Nancy, France
J Biol Chem 280:12344-50. 2005..These results, which show that the N-terminal domain exhibits a disulfide reductase activity and probably has a thioredoxin-fold, are discussed in relation to its possible functional role in Neisseria...
- Inactivation of active and latent transforming growth factor beta by free thiols: potential redox regulation of biological actionRobert Blakytny
Department of Cancer Research, Fachklinik Hornheide, D 48157 Münster, Germany
Int J Biochem Cell Biol 38:1363-73. 2006..g. in burns), as a mechanism protecting from excess TGF-beta activity, as well as in conditions involving redox dysregulation, such as reperfusion injury of the heart, Alzheimer's disease, and cancer...
- Variable reactivity of an engineered cysteine at position 338 in cystic fibrosis transmembrane conductance regulator reflects different chemical states of the thiolXuehong Liu
Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon 97239, USA
J Biol Chem 281:8275-85. 2006..One of the altered states displays reactivity toward thiols like dithiothreitol and 2-mercaptoethanol as well as reagents: mixed disulfides (methanethiosulfonate reagents: MTSET+, MTSES-) ..
- Microsomal UDP-glucuronyltransferase in rat liver: oxidative activationMaría Eugenia Letelier
Laboratory of Pharmacology, Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile
Basic Clin Pharmacol Toxicol 96:480-6. 2005..This activation was prevented or reversed by the reducing agents glutathione, cysteine or dithiothreitol when it was induced by the Fe(3+)/ascorbate...
- Characterization of the redox and metal binding activity of BsSco, a protein implicated in the assembly of cytochrome c oxidaseIveta Imriskova-Sosova
Department of Biochemistry, Queen s University, Kingston, Ontario, K7L 3N6 Canada
Biochemistry 44:16949-56. 2005..Cobalt(II) exhibits weak binding, whereas Ni(II) and Zn(II) do not appear to bind. The high-affinity binding of metals by BsSco is triggered by its redox state, and this property could be important for its function in vivo...
- Reactivities of quinone-free DsbB from Escherichia coliKenji Inaba
Institute for Virus Research, Kyoto University, and CREST of Japan Science and Technology Agency, Kyoto 606 8507, Japan
J Biol Chem 280:33035-44. 2005..redox potentials of cysteine pairs of quinone-free DsbB are lower than that of DsbA, as far as determined in dithiothreitol redox buffer...
- Interactions of sodium selenite, glutathione, arsenic species, and omega class human glutathione transferaseRobert A Zakharyan
Department of Molecular and Cellular Biology, The University of Arizona, Tucson, Arizona 85721 0106, USA
Chem Res Toxicol 18:1287-95. 2005..The inhibition of the MMA(V) reducting activity of hGSTO1-1 by selenite was reversed by 1 mM DL-dithiothreitol (DTT) but not by reduced glutathione (GSH), which is a required substrate for the enzyme...
- Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasisNoriyuki Nagahara
Department of Environmental Medicine, Nippon Medical School, Tokyo 113 8602, Japan
J Biol Chem 280:34569-76. 2005..5 microM and k(i) = 2.5 min(-1), K(i) = 178.6 microM, respectively). The activity was completely restored by dithiothreitol or thioredoxin with a reducing system containing thioredoxin reductase and NADPH, but glutathione did not ..
- Control of virulence in Neisseria meningitidisYih Ling Tzeng; Fiscal Year: 2012..invasion, in regulation of capsule expression as well as the initial identification of a host signal mimic: dithiothreitol (DTT) stress...
- EFFECT OF AGING ON INTERORGAN GLUTATHIONE HOMEOSTASISMurad Ookhtens; Fiscal Year: 1999..2) Perfusions of isolated livers and incubations of freshly-isolated hepatocytes with dithiothreitol (DTT), N- acetylcysteine (NAC) and dimercaprol, in addition to GSH, CYSH and CYSS. 6...
- DEPENDENCE OF P53 ON THIOL MAINTENANCE PROTEINSGary Merrill; Fiscal Year: 2001..determine whether p53 modifications that result in Trr1-independent transcriptional activity in vivo result in dithiothreitol-independent DNA binding activity in vitro...
- DISULFIDE SURFACE STRUCTURE IN LEGIONELLA PATHOGENESISPaul Hoffman; Fiscal Year: 1990..Virulent (V), but not avirulent (AV) isogenic strains release proteins when treated with dithiothreitol suggesting that differences in MOMP structure might correlate with virulence...
- COMPLEX CARBOHYDRATESLAWRENCE MWASI; Fiscal Year: 2002..The enzymatic activity could be kept fairly stable in 20% glycerol containing o.5 mM dithiothreitol. The enzyme showed a pH optimum of about 8.0 in 50 mM Tris buffer...
- Proteomics of Complex 1 Inhibition in GSH-Depleted CellsBradford Gibson; Fiscal Year: 2004..activity appears to be a result of cysteine oxidation which is reversible by the thiol-reducing agent dithiothreitol. These results suggest that early observed GSH losses in PD may be directly responsible for the noted ..
- METALLOPROTEINASE IN HORMONE DEPENDENT BREAST CANCERY Shi; Fiscal Year: 1999..So far, the known inhibitors of the enzyme are EDTA, dithiothreitol, and leuptin...
- PROPERTIES OF THE HIGH-AFFINITY INSULIN RECEPTORMichael Czech; Fiscal Year: 1980..It exists as a disulfide linked complex (300,000 daltons) which is reduced to a 125,000 monomer by dithiothreitol. Development of a selective precipitation procedure to rapidly and specifically resolve the detergent-..
- CANCER CELL-DERIVED PA OF UNUSUALLY LARGE SIZEShashikumar Harvey; Fiscal Year: 1990..2000,000 dalton peptide chain. Reduction with increasing concentrations of dithiothreitol in the presence and absence of SDS will be used to verify this interpretation...
- MECHANISMS OF RADIOPROTECTION & TOXICITY OF SH COMPOUNDSKATHRYN HELD; Fiscal Year: 1992..The thiols to be used are dithiothreitol, glutathione, and cysteamine and its derivatives, including WR-1065...
- MUTATIONAL ANALYSIS OF ANGIOTENSIN II RECEPTORSDaniel Yee; Fiscal Year: 2001..of disulfide bridges and as a source for the differing sensitivities of the two subtypes to the reducing agent dithiothreitol; (iv) identifying the structural elements that are responsible for AT2 subtype selective binding; and (v) ..
- Molecular Neurobiology of Drug AbuseDONALD KUHN; Fiscal Year: 2006..Kuhn will remain extensively engaged in scientific mentoring activities. ..
- NEUROSCIENCE TRAINING IN DRUG ABUSEDONALD KUHN; Fiscal Year: 2004..abstract_text> ..
- PP2A-Like Constitutive ERK/MAPK Phosphatase in BrainTimothy Foley; Fiscal Year: 2003....
- Methamphetamine Neurotoxicity and Microglial ActivationDONALD KUHN; Fiscal Year: 2009..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
- MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTSDONALD KUHN; Fiscal Year: 2002..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
- PKC SIGNALING AND THE TREATMENT OF BIPOLAR DISORDERDONALD KUHN; Fiscal Year: 2002....
- Receptor Affects Fungal Mucosal Colonization in HIVMary Ann Jabra Rizk; Fiscal Year: 2005..dubliniensis to be preferentially selected. ..
- MOLECULAR BASIS OF VIRULENCE FACTORS OF ORAL FUNGAL SP.Mary Ann Jabra Rizk; Fiscal Year: 2006..Long range plans include using the mutants generated to study Candida mannoprotein-specific host immunomodulation, such as stimulation of cytokines and chemokines. ..
- Neutralizing Compounds for Viral Hemorrhagic FeverRichard Larson; Fiscal Year: 2005....
- FUNCTIONAL ARCHITECTURE OF RETINAL ROD CNG ION CHANNELSSHARONA GORDON; Fiscal Year: 2006..Through these experiments we expect to gain significant insight into how the cGMP-gated ion channels function during visual transduction. ..
- MOLECULAR MECHANISMS OF LUNG SURFACTANT SECRETIONLin Liu; Fiscal Year: 2007..Accomplishing the goals of this proposal will give a valuable direction to the therapy of pulmonary diseases such as neonatal respiratory distress syndrome. ..
- DEVELOPMENT OF AN ORAL CARRIERLance L Simpson; Fiscal Year: 2010..To demonstrate an absence of toxicity of the modified carrier and the modified carrier~tetanus antigen construct If successful, this work should demonstrate that a mucosal tetanus vaccine is worthy of evaluation as a clinical product. ..
- Misregulation of apoptosis in cloned pig embryosHeide Schatten; Fiscal Year: 2007..Pilot data from this R03 small grants program research will be used to apply for funding through the R01 mechanism. [unreadable] [unreadable] [unreadable]..
- Nitric oxide and mitochondrial biogenesis in sepsisClaude A Piantadosi; Fiscal Year: 2010..abstract_text> ..
- Aminoglycoside uptake and clearance in mammalian cellsTakatoshi Karasawa; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Antioxidant Interaction of Selenium and Vitamins C & EJames May; Fiscal Year: 2008..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
- Regulation of Oxidative Stress in the mdx DiaphragmJOHN LAWLER; Fiscal Year: 2008..unreadable] [unreadable] [unreadable] [unreadable]..
- Proteasome function in the aging retinaDeborah A Ferrington; Fiscal Year: 2010..Results from these experiments will help verify that immunoproteasome is an essential component of the retinal stress response and plays a fundamental role in maintaining retinal health. ..
- The Effect of Alcohol on NADPH oxidase structure in pancreatic stellate cellsKym Faull; Fiscal Year: 2008..Successful completion of the experiments described in this proposal are essential for the development of targeted therapeutic agents for treatment of alcohol related pancreatitis. [unreadable] [unreadable] [unreadable]..
- Redox Regulation of Cardiac Electrical RemodelingGEORGE ROZANSKI; Fiscal Year: 2009..PHS 398 (Rev. 09/04) Page 2 Principal Investigator/Program Director (Last, first, middle): Rozanski, George, J ..
- Collaborative Development of a Vaccine against Cutaneous and Visceral LeishmaniasMARY EDYTHE WILSON; Fiscal Year: 2010..Through a coordinated approach in the three laboratories, we will systematically perform pre-clinical testing against several Leishmania species, with the goal of developing a pan-leishmania protective vaccine. ..
- Mechanisms of Alveolar Fluid TransportLin Liu; Fiscal Year: 2009..An understanding of the mechanisms of fluid transport in the lung will provide valuable insights and directions toward therapeutic intervention in fetal lung diseases and the resolution of pulmonary edema. ..
- Assay for Formylpeptide Receptor Family Ligands(RMI)Bruce Edwards; Fiscal Year: 2005..We expect that such compounds will prove to be important tools with which to advance the understanding and therapy of inflammation-associated diseases. ..
- Photodynamic therapy of oral candidiasisCONSTANTINE HAIDARIS; Fiscal Year: 2005..2b. To demonstrate proof of principle of PDT in the treatment of C. albicans infection in the oral cavity of rats in vivo. ..
- GP63 EXPRESSION IN LEISHMANIA CHAGASIMary Wilson; Fiscal Year: 2001..They will also provide a model for differential expression of tandemly linked genes in this group of protozoa. ..
- PURCHASE OF MASS SPECTROMETRIC INSTRUMENTATIONKym Faull; Fiscal Year: 2004..Descriptions of the research of the seven major users of the instrument are provided. ..
- NITRIC OXIDE PRODUCTION BY ANG II IN ENDOTHELIUMSusan Olson; Fiscal Year: 2004..These studies are of significant medical importance to heart and pulmonary disease, which involve pathological changes in blood pressure. ..
- RECEPTOR MEDIATED OXIDATIVE STRESSHOWARD SHERTZER; Fiscal Year: 2003..These studies will improve our understanding of how PHAHs such as TCDD generate oxidative stress and mutagenesis, leading to better models for risk assessment and novel strategies for intervention in PHAH-mediated toxicity. ..
- Cytoskeletal organization in apicomplexan parasitesHeide Schatten; Fiscal Year: 2005..Pilot data from this RO3 research will be used to apply for funding through the RO1 mechanism. ..
- Prevention of Recombinant Protein Aggregation in E.coliShaorong Chong; Fiscal Year: 2005..As a long-term goal, this project aims to provide insights into the mechanism of the aggregation by these disease-related proteins and the means to prevent aggregation. ..
- Immunogenetics of Leishmania chagasi InfectionMary Wilson; Fiscal Year: 2005..chagasi infection, and to identify specific alleles that influence human immune responses and thereby modify the course of disease. ..
- OXIDATIVE DNA DAMAGE AND REPAIR IN CNS CELLSMark Kelley; Fiscal Year: 2003..abstract_text> ..
- The Major Surface Protease of Trypanosomatid ProtozoaMary Wilson; Fiscal Year: 2003..chagasi whose coding sequences predict they lack the usual GPI anchor, and to contrast the amino acid sequences that regulate the intracellular trafficking of these unusual MSPs with the MSPs that possess a GPI membrane anchor. ..
- Molecular determinants of ACTH receptor for ligand bindingYingkui Yang; Fiscal Year: 2006..The information will serve to establish a foundation on which to build a comprehensive understanding of the signaling events that regulate ACTH mediated adrenal function in physiologic and diseased states. ..
- Lung cancer chemoprevention and tocopheryl succinateRichard Wright; Fiscal Year: 2005..The overall goal of this work is that it may contribute to an eventual reduction in the incidence or severity of human lung cancers. ..
- PHybrid Quadrupole/Orthogonal Time-of-Flight Mass SpecKym Faull; Fiscal Year: 2002..Reflecting this diversity of use, descriptions of the research of the major users of the instrument are provided...
- Functional Organization of the Myelin MembraneChristopher Taylor; Fiscal Year: 2003..in forming rafts in myelin, 3) provide a more complete understanding of the organization of proteins and lipids in myelin as well as identify potential targets for the therapeutic intervention in many myelin related disorders/diseases ..
- Structure of the Glycine Receptor's Alcohol Binding SiteINGRID LOBO; Fiscal Year: 2003..abstract_text> ..
- Role of the Unfolded Protein Response in Type 2 DiabetesFEROZ PAPA; Fiscal Year: 2008..The UCSF Department of Medicine is fully committed to Dr. Papa's career development, and will make all necessary resources available to facilitate the success of the project. ..
- Discriminative Stimulus Effects of Alcohol in MiceWouter Koek; Fiscal Year: 2007..This future research will increase our understanding of risk factors for alcoholism and drug abuse and will help the development of effective approaches to prevention and treatment. [unreadable] [unreadable] [unreadable]..
- Biophysics of CNS ConnexinsVYTAUTAS VERSELIS; Fiscal Year: 2006..abstract_text> ..
- Proteomics of altered protein in macular degenerationDEBORAH FERRINGTON; Fiscal Year: 2006..Identification of proteins that are uniquely altered will help provide valuable insight into the mechanism of AMD. ..
- Therapeutic/Mechanistic Role of Ape1 in Germ Cell TumorsMark Kelley; Fiscal Year: 2007..If any mutants are shown to sensitize the GCT cell lines to chemo-/IR agents, as expected, this will set the stage for future gene therapy approaches to sensitize GCT's to therapy. ..
- Mechanisms of Alveolar Epithelial Cell DifferentiationLin Liu; Fiscal Year: 2006..abstract_text> ..
- "Ligand Independent" endocrine disruption by pesticidesPatrick Wong; Fiscal Year: 2005..abstract_text> ..
- Agmatine Mediated Arrest of ProliferationJoseph Satriano; Fiscal Year: 2003..UCSD has established itself as a leading academic institution and thus provides an excellent environment for the exchange of ideas and techniques essential for the growth of the PI's career. ..
- ALDOSTERONE, HYPERTENSION AND THE BRAINElise Gomez Sanchez; Fiscal Year: 2003..4) Study of the metabolism of aldosterone and corticosterone and the regulation of the synthesis of low polarity derivatives in the brain, including the identification of the sites of formation of aldosterone acyl esters. ..
- CYTOMIMETIC ORGANIC CHEMISTRYFREDRIC MENGER; Fiscal Year: 2007....
- Probing the pore of CFTR using chemical modificationXuehong Liu; Fiscal Year: 2003..mechanism to explain how HFS type diets cause insulin resistance. and hypertension, and how a LFCC diet, antioxidant therapy or exercise training aid in the control of insulin resistance and hypertension. ..
- Mitochondria distribution in cloned pig embryosHeide Schatten; Fiscal Year: 2005..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
- A tyrosinase-targeted drug design for the treatment of melanomaMajid Moridani; Fiscal Year: 2007..In this work we will be developing novel phenol based drugs for treatment of melanoma. [unreadable] [unreadable] [unreadable]..
- Redox Control of Radiation ResponseJohn Biaglow; Fiscal Year: 2006..Ultimately, we hope to develop specific strategies for modifying thiol redox homeostasis in vivo, to make human tumors more sensitive to ionizing radiation. ..
- MOLECULAR MECHANISMS OF PACEMAKER CHANNEL FUNCTIONMichael Sanguinetti; Fiscal Year: 2008..These studies promise to further our understanding of the biological oscillator that controls heart rhythm. ..