dithiothreitol

Summary

Summary: A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols.

Top Publications

  1. ncbi Redox regulation of the DNA repair function of the human AP endonuclease Ape1/ref-1
    M R Kelley
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA
    Antioxid Redox Signal 3:671-83. 2001
  2. ncbi High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol
    Shixuan Wu
    U S Department of Agriculture, Laramie, WY, USA
    Biotechniques 36:152-4. 2004
  3. ncbi Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol
    Keith Vosseller
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Proteomics 5:388-98. 2005
  4. pmc Vitamin K epoxide reductase contributes to protein disulfide formation and redox homeostasis within the endoplasmic reticulum
    Lori A Rutkevich
    Department of Biochemistry, University of Toronto, Toronto, ON, Canada
    Mol Biol Cell 23:2017-27. 2012
  5. ncbi Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response
    Hiromi Tajima
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Biochem Biophys Res Commun 374:242-7. 2008
  6. pmc Structural perturbation and enhancement of the chaperone-like activity of alpha-crystallin by arginine hydrochloride
    Volety Srinivas
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    Protein Sci 12:1262-70. 2003
  7. pmc Reversal by dithiothreitol treatment of the block in murine leukemia virus maturation induced by disulfide cross-linking
    Stephen Campbell
    HIV Drug Resistance Program, SAIC Frederick, National Cancer Institute Frederick, Natipnal Institutes of Health, Frederick, Maryland 21702, USA
    J Virol 76:10050-5. 2002
  8. ncbi Effects of oocyte activation and sperm preparation on the development of porcine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm injection
    Jian Hui Tian
    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, PR China
    Theriogenology 66:439-48. 2006
  9. ncbi Is dithiothreitol affecting cells and soluble mediators during sputum processing? A modified methodology to process sputum
    Patrizia Pignatti
    J Allergy Clin Immunol 110:667-8. 2002
  10. ncbi The secretory pathway is normal in dithiothreitol-treated cells, but disulfide-bonded proteins are reduced and reversibly retained in the endoplasmic reticulum
    H F Lodish
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    J Biol Chem 268:20598-605. 1993

Research Grants

Detail Information

Publications244 found, 100 shown here

  1. ncbi Redox regulation of the DNA repair function of the human AP endonuclease Ape1/ref-1
    M R Kelley
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA
    Antioxid Redox Signal 3:671-83. 2001
    ..These findings suggest that the Apel/ref-1 protein may be much more intimately regulated at the posttranslational level than initially imagined...
  2. ncbi High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol
    Shixuan Wu
    U S Department of Agriculture, Laramie, WY, USA
    Biotechniques 36:152-4. 2004
    ..We report here that pretreatment of P. pastoris with 0.1 M lithium acetate (LiAc) and 10 mM dithiothreitol (DTT) before electroporation increased transformation efficiency approximately 150-fold...
  3. ncbi Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol
    Keith Vosseller
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Proteomics 5:388-98. 2005
    ..The method (BEMAD) involves differential isotopic labeling through Michael addition with normal dithiothreitol (DTT) (d0) or deuterated DTT (d6), and enrichment of these peptides by thiol chromatography...
  4. pmc Vitamin K epoxide reductase contributes to protein disulfide formation and redox homeostasis within the endoplasmic reticulum
    Lori A Rutkevich
    Department of Biochemistry, University of Toronto, Toronto, ON, Canada
    Mol Biol Cell 23:2017-27. 2012
    ..In contrast, no involvement of QSOX1 in ER oxidative processes could be detected. These findings establish VKOR as a significant contributor to disulfide bond formation within the ER...
  5. ncbi Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response
    Hiromi Tajima
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Biochem Biophys Res Commun 374:242-7. 2008
    ..conditions, GFP fluorescence localization almost overlapped with an ER marker; however, tunicamycin and dithiothreitol treatment clearly increased GFP fluorescence in the nucleus suggesting that the N-terminal fragment of ..
  6. pmc Structural perturbation and enhancement of the chaperone-like activity of alpha-crystallin by arginine hydrochloride
    Volety Srinivas
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    Protein Sci 12:1262-70. 2003
    ..This study should prove useful in designing strategies to improve chaperone function for therapeutic applications...
  7. pmc Reversal by dithiothreitol treatment of the block in murine leukemia virus maturation induced by disulfide cross-linking
    Stephen Campbell
    HIV Drug Resistance Program, SAIC Frederick, National Cancer Institute Frederick, Natipnal Institutes of Health, Frederick, Maryland 21702, USA
    J Virol 76:10050-5. 2002
    ..We now show that treatment of these immature particles with a reducing agent (dithiothreitol) induces their maturation in vitro, as evidenced by proteolytic cleavage of Gag, Gag-Pol, and Env proteins and ..
  8. ncbi Effects of oocyte activation and sperm preparation on the development of porcine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm injection
    Jian Hui Tian
    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, PR China
    Theriogenology 66:439-48. 2006
    ..freeze-thaw cycles (without cryoprotectant) on oocyte activation and the effects of sperm pre-incubated with dithiothreitol (DTT) or Triton X-100 on early embryo development were also examined...
  9. ncbi Is dithiothreitol affecting cells and soluble mediators during sputum processing? A modified methodology to process sputum
    Patrizia Pignatti
    J Allergy Clin Immunol 110:667-8. 2002
  10. ncbi The secretory pathway is normal in dithiothreitol-treated cells, but disulfide-bonded proteins are reduced and reversibly retained in the endoplasmic reticulum
    H F Lodish
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    J Biol Chem 268:20598-605. 1993
    Addition of 2 mM dithiothreitol (DTT) to HepG2 human hepatoma cells blocks secretion of newly made albumin and causes it to accumulate in the endoplasmic reticulum in the reduced state...
  11. ncbi Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity
    Utpal B Pajvani
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:9073-85. 2003
    ..Surprisingly, Acrp30(C39S) or wild-type Acrp30 treated with dithiothreitol are significantly more bioactive than the higher order oligomeric forms of the protein with respect to ..
  12. ncbi Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulum
    M G Pollard
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143 0450, USA
    Mol Cell 1:171-82. 1998
    ..We conclude that there exists a specific cellular redox machinery required for disulfide-linked protein folding in the ER and that Ero1p is an essential component of this machinery...
  13. ncbi Dithiothreitol abrogates the effect of arsenic trioxide on normal rat liver mitochondria and human hepatocellular carcinoma cells
    Manash K Paul
    Department of Biology, Indian Institute of Science Education and Research, Mohali, MGSIPA Complex, Adjacent Sacred Heart School, Sector 26, Chandigarh 160019 corrected India
    Toxicol Appl Pharmacol 226:140-52. 2008
    ..b>Dithiothreitol (DTT) is a well-known dithiol agent reported to modulate the action of ATO...
  14. ncbi Cysteine-25 of adenylate kinase reacts with dithiothreitol to form an adduct upon aging of the enzyme
    X Li
    National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, 15 Datun Road, Beijing 100101, PR China
    FEBS Lett 507:169-73. 2001
    Adenylate kinase (AK) ages in solution in the presence of DL-dithiothreitol (DTT) with a gradual activity decrease...
  15. ncbi High efficiency transformation of Schizosaccharomyces pombe pretreated with thiol compounds by electroporation
    M Suga
    Department of Materials and Biosystem Engineering, Toyama University, 3190 Gofuku, Toyama City, Toyama 930 8555, Japan
    Yeast 18:1015-21. 2001
    ..Among the thiol compounds tested, dithiothreitol (DTT) was the most effective for pretreatment...
  16. ncbi Bovine embryo development following ICSI: effect of activation, sperm capacitation and pre-treatment with dithiothreitol
    Cesare Galli
    Laboratorio di Tecnologie della Riproduzione, CIZ srl, Istituto Sperimentale Italiano Lazzaro Spallanzani, Via Porcellasco 7 f, 26100 Cremona, Italy
    Theriogenology 60:1467-80. 2003
    ..Finally, we studied the effect of dithiothreitol (DTT) pre-treatment of sperm or of injected oocytes...
  17. ncbi Reversible inhibitors of lambda integrase-mediated recombination efficiently trap Holliday junction intermediates and form the basis of a novel assay for junction resolution
    Jeffrey L Boldt
    Department of Biology and Center for Microbial Sciences, San Diego State University, San Diego, California 92182 4614, USA
    J Biol Chem 279:3472-83. 2004
    ..This assay is particularly useful for determining junction resolution rates in cases where complexes directly assembled on junction substrates undergo little or no catalysis...
  18. ncbi A new cell culture-based assay quantifies vitamin K 2,3-epoxide reductase complex subunit 1 function and reveals warfarin resistance phenotypes not shown by the dithiothreitol-driven VKOR assay
    A Fregin
    Institute of Human Genetics, University of Wurzburg, Wurzburg, Germany
    J Thromb Haemost 11:872-80. 2013
    ..VKORC1 function has historically been assessed in vitro using a dithiothreitol (DTT)-driven vitamin K 2,3-epoxide reductase (VKOR) assay...
  19. ncbi Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII intein
    Kenneth V Mills
    College of the Holy Cross, Department of Chemistry, Worcester, Massachusetts 01610, USA
    J Biol Chem 280:2714-20. 2005
    ..The pseudo first order rate constant of dithiothreitol-dependent N-terminal cleavage is 1 x 10(-4) s(-1). The first order rate constant of C-terminal cleavage is 1...
  20. ncbi Sperm movement in the ooplasm, dithiothreitol pretreatment and sperm freezing are not required for the development of porcine embryos derived from injection of head membrane-damaged sperm
    Hwan Yul Yong
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 63:783-94. 2005
    The present study investigated the correlation of sperm movement in the ooplasm, pretreatment of sperm with dithiothreitol (DTT) and sperm freezing with the development of porcine embryos derived from modified intracytoplasmic sperm ..
  21. ncbi Effects of dithiothreitol and boar on pronuclear formation and embryonic development following intracytoplasmic sperm injection in pigs
    Hiroyuki Watanabe
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 8555, Japan
    Theriogenology 65:528-39. 2006
    ..fertilization, male pronuclear formation and embryonic development following intracytoplasmic injection of dithiothreitol (DTT)-treated boar spermatozoa...
  22. ncbi UPR-independent dithiothreitol stress-induced genes in Aspergillus niger
    D A MacKenzie
    Department of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
    Mol Genet Genomics 274:410-8. 2005
    A subtraction library was prepared from cultures of Aspergillus niger that had or had not been exposed to dithiothreitol (DTT), in order to identify genes involved in the unfolded protein response (UPR) or in the response to reductive ..
  23. ncbi Selective retention of secretory proteins in the yeast endoplasmic reticulum by treatment of cells with a reducing agent
    E Jämsä
    Institute of Biotechnology, University of Helsinki, Finland
    Yeast 10:355-70. 1994
    ..Treatment of living cells with the reducing agent dithiothreitol (DTT) prevented disulfide formation of newly synthesized CPY and hsp150, resulting in retention of the ..
  24. ncbi Ascorbic and dehydroascorbic acids measured in plasma preserved with dithiothreitol or metaphosphoric acid
    S A Margolis
    Organic Analytical Chemistry Division, National Institute for Standards and Technology, Gaithersburg, MD 20899
    Clin Chem 36:1750-5. 1990
    ..83 mg/L. The stability and homogeneity of lyophilized plasma samples supplemented with ascorbic acid and dithiothreitol are documented...
  25. ncbi Mechanism of suppression of dithiothreitol-induced aggregation of bovine alpha-lactalbumin by alpha-crystallin
    Zoya M Bumagina
    A N Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia
    Biophys Chem 146:108-17. 2010
    The kinetics of dithiothreitol (DTT)-induced aggregation of alpha-lactalbumin from bovine milk has been studied using dynamic light-scattering technique...
  26. ncbi Different activation treatments for successful development of bovine oocytes following intracytoplasmic sperm injection
    S A Ock
    Gyeongsang National University, Republic of Korea and Madras Veterinary College, Tamil Nadu, India
    Zygote 11:69-76. 2003
    ..Motile spermatozoa selected by Percoll density gradient were treated with 5 mM dithiothreitol (DTT) and analysed for ultrastructural changes of the head using transmission electron microscopy (TEM)...
  27. ncbi Quantifying dithiothreitol displacement of functional ligands from gold nanoparticles
    De Hao Tsai
    Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Anal Bioanal Chem 404:3015-23. 2012
    b>Dithiothreitol (DTT)-based displacement is widely utilized for separating ligands from their gold nanoparticle (AuNP) conjugates, a critical step for differentiating and quantifying surface-bound functional ligands and therefore the ..
  28. ncbi Self-arrangement among charge-stabilized gold nanoparticles on a dithiothreitol reactivated octanedithiol monolayer
    Anders O Lundgren
    Department of Cell and Molecular Biology, Interface Biophysics, Goteborg University, SE 405 30 Goteborg, Sweden
    Nano Lett 8:3989-92. 2008
    Gold surfaces and structures modified with octanedithiol were reacted with dithiothreitol prior to immersion in buffered solutions of charge stabilized gold nanoparticles...
  29. ncbi Dependence of product formation from decomposition of nitroso-dithiols on the degree of nitrosation. Evidence that dinitroso-dithiothreitol acts solely as an nitric oxide releasing compound
    Sonja Liebeskind
    Institut fur Physiologische Chemie, Universitatsklinikum Essen, Hufelandstrasse 55, 45122, Essen, Germany
    Org Biomol Chem 6:2560-73. 2008
    ..In this paper, we demonstrate that nitrosated dl-dithiothreitol (DTT) decays via two independent pathways, that is, one producing exclusively nitric oxide and one producing (..
  30. ncbi Significant increases in potato Starch-Synthase and Starch-Branching-Enzyme activities by dilution with buffer containing dithiothreitol and polyvinyl alcohol 50 K
    Rupendra Mukerjea
    Laboratory of Carbohydrate Chemistry and Enzymology, The Roy J Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Carbohydr Res 367:25-8. 2013
    ..5), containing 1.0mM dithiothreitol (DTT) and 0...
  31. pmc Substitution of serine for proline in the active center of type 2 iodothyronine deiodinase substantially alters its in vitro biochemical properties with dithiothreitol but not its function in intact cells
    Iuri Martin Goemann
    Thyroid Section, Endocrine Division, Hospital de Clinicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035 903, Brazil
    Endocrinology 151:821-9. 2010
    ..show that despite an approximately 2000-fold higher Michaelis constant (K(m); T(4)) for D1 than for D2 using dithiothreitol (DTT) as cofactor, D1 expressed in intact cells produces T(3) at free T(4) concentrations many orders of ..
  32. ncbi Synergistic cytotoxic effects of arsenic trioxide plus dithiothreitol on mice oral cancer cells
    Chia Wen Tsai
    Terry Fox Cancer Research Lab, Graduate Institute of Chinese Medical Science, School of Medicine, China Medical University, Taichung, 404 Taiwan, ROC
    Anticancer Res 30:3655-60. 2010
    ..The results showed that 10-20 μM dithiothreitol enhanced the cytotoxic effects of arsenic trioxide to 43.3~62...
  33. ncbi Reducing agent dithiothreitol facilitates activity of the capsaicin receptor VR-1
    L Vyklicky
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Neuroscience 111:435-41. 2002
    ..On the extracellular side, VR1 has three cysteine residues at positions 616, 621 and 634. Here we report that dithiothreitol (DTT) (2-60 mM), an agent that maintains -SH groups of cysteines in a reduced state, greatly facilitates ..
  34. ncbi Redox/pH dual stimuli-responsive biodegradable nanohydrogels with varying responses to dithiothreitol and glutathione for controlled drug release
    Yuan Jia Pan
    State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China
    Biomaterials 33:6570-9. 2012
    ..1) in the presence of 10 mM dithiothreitol (DTT) or glutathione (GSH)...
  35. ncbi Modification of actions of heat shock on development and apoptosis of cultured preimplantation bovine embryos by oxygen concentration and dithiothreitol
    L A de Castro e Paula
    Department of Animal Sciences, University of Florida, Gainesville, Florida, USA
    Mol Reprod Dev 75:1338-50. 2008
    ..low oxygen environment (5% oxygen) and (2) that these effects would be reversed by addition of the antioxidant dithiothreitol (DTT)...
  36. ncbi Direct evidence that two cysteines in the dopamine transporter form a disulfide bond
    Rong Chen
    Department of Pharmacology, The Ohio State University College of Medicine, 333 West 10th Avenue, Columbus, OH 43210, USA
    Mol Cell Biochem 298:41-8. 2007
    ..Our results and previous results are consistent with the notion that the disulfide bond between EL2 cysteines is required for DAT biosynthesis and/or its delivery to the cell surface...
  37. ncbi Beneficial effects of dithiothreitol on relative levels of glutathione S-transferase activity and thiols in oocytes, and cell number, DNA fragmentation and allocation at the blastocyst stage in the mouse
    Francisco Rausell
    Department of Functional Biology and Physical Anthropology, Faculty of Biological Sciences, University of Valencia, Valencia, Spain
    Mol Reprod Dev 74:860-9. 2007
    We analyzed the effect of in vitro aging of mouse oocytes in the presence of dithiothreitol (DTT) on relative levels of glutathione S-transferase (GST) activity and thiols in oocytes, and cell number, DNA fragmentation and cellular ..
  38. ncbi Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803
    D D Martin
    New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
    Biochemistry 40:1393-402. 2001
    ..Observed rate constants (k(obs)) for dithiothreitol-induced N-terminal cleavage, C-terminal cleavage, and trans-splicing were (1.0 +/- 0.5) x 10(-3), (1.9 +/- 0...
  39. ncbi Additive effects of bovine serum albumin, dithiothreitol, and glycerol on PCR
    M Nagai
    Department of Biochemistry, Iwate Medical University School of Dentistry, Japan
    Biochem Mol Biol Int 44:157-63. 1998
    The effects of bovine serum albumin, dithiothreitol, and glycerol on PCR were studied. PCR under standard conditions failed the amplification of an enterohemorrhagic E...
  40. ncbi A sulfhydryl oxidase from chicken egg white
    K L Hoober
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Biol Chem 271:30510-6. 1996
    ..b>Dithiothreitol was the best substrate of those tested, with a turnover number of 1030/min, a Km of 150 microM, and a pH ..
  41. ncbi Arginine hydrochloride enhances the dynamics of subunit assembly and the chaperone-like activity of alpha-crystallin
    V Srinivas
    Centre for Cellular and Molecular Biology, Hyderabad, India
    Mol Vis 11:249-55. 2005
    ..The purpose of the present study is to get an insight into the mechanism of the arginine induced enhancement of chaperone-like activity of alpha-crystallin...
  42. ncbi Insights into hydrophobicity and the chaperone-like function of alphaA- and alphaB-crystallins: an isothermal titration calorimetric study
    M Satish Kumar
    Biochemistry Division, National Institute of Nutrition, Hyderabad 500 007, India
    J Biol Chem 280:21726-30. 2005
    ..Our results indicate that although hydrophobicity appears to be a factor in determining the chaperone-like activity of alpha-crystallins, it does not quantitatively correlate with the chaperone function of alpha-crystallins...
  43. pmc The activity-induced reduction of myofibrillar Ca2+ sensitivity in mouse skeletal muscle is reversed by dithiothreitol
    Terence R Moopanar
    Institute for Biomedical Sciences, School of Medical Sciences, University of Sydney F13, NSW 2006, Australia
    J Physiol 571:191-200. 2006
    ..ii) If the membrane-permeant thiol-specific reducing agent dithiothreitol (0...
  44. ncbi Interaction of metals and thiols in cell damage and glutathione distribution: potentiation of mercury toxicity by dithiothreitol
    B Hultberg
    Department of Clinical Chemistry, Institute of Laboratory Medicine, University Hospital, S-22185 Lund, Sweden
    Toxicology 156:93-100. 2001
    ..The addition of dithiothreitol (DTT) but not the other thiols potentiated the effects of mercury ions on glutathione distribution and cell ..
  45. ncbi Effect of phosphorylation on alpha B-crystallin: differences in stability, subunit exchange and chaperone activity of homo and mixed oligomers of alpha B-crystallin and its phosphorylation-mimicking mutant
    Md Faiz Ahmad
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    J Mol Biol 375:1040-51. 2008
    ..We propose that the degree of phosphorylation of the alpha B-crystallin oligomers and temperature are key modulators to achieve a wide range of chaperone capabilities of the small heat shock protein, alpha -crystallin...
  46. ncbi Optimized dialysis and protease inhibition of sputum dithiothreitol supernatants
    Edward M Erin
    NHLI Clinical Studies Unit, Royal Brompton Hospital, Fulham Road, London SW3 6HP, UK
    Am J Respir Crit Care Med 177:132-41. 2008
    b>Dithiothreitol (DTT) is commonly used to liquefy induced sputum samples before assessment of cytology, but causes reduction of disulfide bonds and denaturation of proteins.
  47. ncbi Purification and characterization of a new trypsin inhibitor from Dimorphandra mollis seeds
    G C Mello
    Departamento de Bioquimica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil
    J Protein Chem 20:625-32. 2001
    ..The inhibitory activity was stable over a wide pH range and in the presence of DTT. The N-terminal sequence of DMTI-II showed a high degree of homology with other Kunitz-type inhibitors...
  48. pmc Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and bronchiectasis
    I S Woolhouse
    Department of Medicine, Queen Elizabeth Hospital, Birmingham, UK
    Thorax 57:667-71. 2002
    ..However, it is not known whether dithiothreitol (DTT), a reducing mucolytic agent regularly used to homogenise sputum, affects the detection of inflammatory ..
  49. ncbi Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression
    Nicole Magnasco Nichols
    New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
    Biochemistry 42:5301-11. 2003
    ..By inhibiting both the splicing pathway and spontaneous hydrolysis with Zn(2+), reactants can be diverted from the trans splicing to the trans cleavage pathway where DTT and EDTA can regulate N- and C-terminal cleavage, respectively...
  50. ncbi Role of disulfide bonds in Acrp30/adiponectin structure and signaling specificity. Different oligomers activate different signal transduction pathways
    Tsu Shuen Tsao
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    J Biol Chem 278:50810-7. 2003
    ..HMW) forms of Acrp30 depends upon formation of Cys22-mediated disulfide bonds because their reduction with dithiothreitol or substitution of Cys22 with alanine led exclusively to trimers...
  51. ncbi Conformational change and inactivation of arginine kinase from shrimp Feneropenaeus chinensis in oxidized dithiothreitol solutions
    Ji Cheng Pan
    Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People s Republic of China
    Biochem Cell Biol 82:361-7. 2004
    The effect of oxidized dithiothreitol (DTT) on the conformation and function of arginine kinase from shrimp Feneropenaeus chinensis was investigated with the methods of intrinsic fluorescence, ANS fluorescence, size exclusion ..
  52. ncbi Caffeine and dithiothreitol delay ovine oocyte ageing
    Xiao Fang Ye
    Xinjiang Key Laboratory of Animal Biotechnology, Xinjiang Academy of Animal Sciences, Urumqi 830000, China
    Reprod Fertil Dev 22:1254-61. 2010
    ..somatic cell nuclear transfer and intracytoplasmic sperm injection in the presence or absence of caffeine or dithiothreitol (DTT) were examined...
  53. ncbi A bio-bar-code assay based upon dithiothreitol-induced oligonucleotide release
    C Shad Thaxton
    Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    Anal Chem 77:8174-8. 2005
    ..on the ability to liberate the adsorbed thiolated oligonucleotides from the gold nanoparticle surface with dithiothreitol (DTT), which simplifies the assay and increases its quantitative capabilities...
  54. ncbi The papaya Kunitz-type trypsin inhibitor is a highly stable beta-sheet glycoprotein
    Mohamed Azarkan
    University of Brussels, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme CP 609, 808, Route de Lennik, Bz 1070 Brussels, Belgium
    Biochim Biophys Acta 1764:1063-72. 2006
    ..Exposure to 2.5 mM dithiothreitol for 120 min caused a 33% loss of the inhibitory activity, while a loss of 75% was obtained in the presence of ..
  55. ncbi Reduction with dithiothreitol causes serglycin-specific defects in secretory granule integrity of bone marrow derived mast cells
    Tiago Braga
    Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, Uppsala, Sweden
    Mol Immunol 46:422-8. 2009
    ..Interference with such motifs of neuroendocrine cells with dithiothreitol (DTT) has previously been shown to cause cellular missorting...
  56. ncbi The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum
    A R Frand
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Mol Cell 1:161-70. 1998
    ..We show that glutathione is not required for CPY folding and conclude that Ero1p functions in a novel mechanism that sustains the ER oxidizing potential, supporting net formation of protein disulfide bonds...
  57. ncbi Dynamic rearrangement of the outer mouth of a K+ channel during gating
    Y Liu
    Department of Neurobiology, Harvard Medical School, Massachusetts General Hospital, Boston 02114, USA
    Neuron 16:859-67. 1996
    ..These results give a consistent picture in which C-type inactivation promotes a local rearrangement and constriction of the channel at the outer mouth...
  58. ncbi Structure and mechanism of peptide methionine sulfoxide reductase, an "anti-oxidation" enzyme
    W T Lowther
    Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 39:13307-12. 2000
    ..The structures support a unique, thiol-disulfide exchange mechanism that relies upon an essential cysteine as a nucleophile and additional conserved residues that interact with the oxygen atom of the sulfoxide moiety...
  59. ncbi Isolation and characterization of a trypsin-chymotrypsin inhibitor from the seeds of green lentil (Lens culinaris)
    Allen H K Cheung
    Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Protein Pept Lett 14:859-64. 2007
    ..The trypsin inhibitory activity of the inhibitor was sensitive to the reducing agent dithiothreitol. It was completely abrogated after treatment with 10 mM dithiothreitol for 20 minutes...
  60. ncbi The mode of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallin
    F A Bettelheim
    Chemistry Department, Adelphi University, Garden City, New York, 11530, USA
    Biochem Biophys Res Commun 261:292-7. 1999
    ..The chaperoned complex (alpha-crystallin plus alpha-lactalbumin) is in dynamic equilibrium both with the monomeric and the aggregated alpha-lactalbumin population...
  61. ncbi Botulinum neurotoxin types A, B, and E: fragmentations by autoproteolysis and other mechanisms including by O-phenanthroline-dithiothreitol, and association of the dinucleotides NAD(+)/NADH with the heavy chain of the three neurotoxins
    Bibhuti R Dasgupta
    Department of Food Microbiology and Toxicology, University of Wisconsin Madison, 1925 Willow Drive, Madison, WI 53706, USA
    Protein J 24:337-68. 2005
    ..The metal chelator O-phenanthroline (above critical miceller concentration) in the presence of dithiothreitol cleaved type E NT at limited sites generating discrete 114-, 87-, 49-, 42-, and 31-kDa fragments but degraded ..
  62. ncbi Combination of dithiothreitol and detergent treatment of spermatozoa causes paternal chromosomal damage
    Monika A Szczygiel
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
    Biol Reprod 67:1532-7. 2002
    Treatment of spermatozoa with either the nonionic detergent Triton X-100 (TX) or dithiothreitol (DTT) has been suggested to confer enhanced success on intracytoplasmic sperm injection (ICSI) in mice and humans...
  63. pmc Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo
    D Missiakas
    Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132
    Proc Natl Acad Sci U S A 90:7084-8. 1993
    ..coli from the lethal effects of dithiothreitol (DTT), and (ii) screening of insertion libraries of E. coli for DTT-sensitive mutants...
  64. ncbi Dithiothreitol induces the sacrificial antioxidant property of human serum albumin in a metal-catalyzed oxidation and gamma-irradiation system
    I G Kim
    Department of Radiation Biology, Korea Atomic Energy Research Institute, Yusong, Taejon
    Arch Biochem Biophys 388:1-6. 2001
    ..Intact or Cys-34-modified human serum albumin (HSA) was significantly degraded in the MCO system containing dithiothreitol (DTT) as electron donor, but as long as it lasted, HSA prohibited *OH or H2O2 from initiating molecular damage ..
  65. pmc High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol
    Maria Papagianni
    Department of Hygiene and Technology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece
    BMC Biotechnol 7:15. 2007
    ..In the present study, treatment with lithium acetate (LiAc) and dithiothreitol (DTT) in various combinations was applied to L. lactis spp...
  66. ncbi Suppression of DTT-induced aggregation of abrin by alphaA- and alphaB-crystallins: a model aggregation assay for alpha-crystallin chaperone activity in vitro
    G Bhanuprakash Reddy
    Biochemistry Division, National Institute of Nutrition, Hyderabad 500 007, India
    FEBS Lett 522:59-64. 2002
    ..In this study, we demonstrate that dithiothreitol (DTT)-induced aggregation of abrin B-chain could be monitored by light scattering similar to that of insulin...
  67. ncbi Cancer-preventive selenocompounds induce a specific redox modification of cysteine-rich regions in Ca(2+)-dependent isoenzymes of protein kinase C
    R Gopalakrishna
    Department of Cell and Neurobiology, School of Medicine, University of Southern California, Los Angeles 90033, USA
    Arch Biochem Biophys 348:25-36. 1997
    ....
  68. ncbi Kinetics of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallin
    Frederick A Bettelheim
    Chemistry Department, Adelphi University, Garden City, NY 11530, USA
    Int J Biol Macromol 30:161-9. 2002
    ..k(4) is somewhat faster than k(3) and it is three times faster than k(s) governing the self-aggregation of lactalbumin in the absence of alpha-crystallin...
  69. ncbi Dithiothreitol causes HIV-1 integrase dimer dissociation while agents interacting with the integrase dimer interface promote dimer formation
    Manuel Tsiang
    Gilead Sciences, 333 Lakeside Drive, Foster City, California 94404, United States
    Biochemistry 50:1567-81. 2011
    ..Surprisingly, we found that dithiothreitol (DTT), a reducing agent essential for in vitro enzymatic activity of IN, weakened the interaction between IN ..
  70. ncbi Increased concentrations of radioisotopically-labeled complementary ribonucleic acid probe, dextran sulfate, and dithiothreitol in the hybridization buffer can improve results of in situ hybridization histochemistry
    Erik Hrabovszky
    Department of Biology, University of Massachusetts, Amherst, Massachusetts, USA
    J Histochem Cytochem 50:1389-400. 2002
    ..signals resulting when varying concentrations of (35)S-labeled cRNA probes, dextran sulfate (DS), and dithiothreitol (DTT) were used for ISHH...
  71. pmc Paradoxical acceleration of dithiothreitol-induced aggregation of insulin in the presence of a chaperone
    Zoya Bumagina
    A N Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia E Mails Z B N A B K
    Int J Mol Sci 11:4556-79. 2010
    The kinetics of dithiothreitol (DTT)-induced aggregation of human recombinant insulin and the effect of α-crystallin, a representative of the family of small heat shock proteins, on the aggregation process have been studied using dynamic ..
  72. ncbi Lipid peroxidation-dependent and -independent protein thiol modifications in isolated rat hepatocytes: differential effects of vitamin E and disulfiram
    P Dogterom
    Division of Toxicology, University of Leiden, The Netherlands
    Chem Biol Interact 71:291-306. 1989
    ..in cells, decreased to 50% by exposure to AA, DEM and BIU, could be reversed to 75% of the initial level by dithiothreitol (DTT) treatment, indicating that the protein thiols were partially modified into disulfides and partially into ..
  73. ncbi Tris(2-carboxyethyl)phosphine stabilization of RNA: comparison with dithiothreitol for use with nucleic acid and thiophosphoryl chemistry
    Steven S Rhee
    Department of Chemistry, Indiana University, Bloomington, IN 47405 7102, USA
    Anal Biochem 325:137-43. 2004
    ..The sulfhydryls of dithiothreitol (DTT) compete with thiophosphates for binding to the mercury within [(N-acryloylamino)phenyl] mercuric ..
  74. ncbi Coordination of heavy metals by dithiothreitol, a commonly used thiol group protectant
    A Kr zel
    Faculty of Chemistry, , Poland
    J Inorg Biochem 84:77-88. 2001
    D,L-Dithiothreitol (DTT), known also as Cleland reagent, is a thiol group protectant, used commonly in peptide and protein chemistry...
  75. ncbi Production of intracellular superoxide mediates dithiothreitol-dependent inhibition of apoptotic cell death
    Marie Veronique Clement
    Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore 117 597
    Antioxid Redox Signal 7:456-64. 2005
    ..of cysteine residues in and around the catalytic site of the proteases, cysteine- reducing agents such as dithiothreitol (DTT) are expected to facilitate caspase activity upon induction of apoptosis...
  76. ncbi Electrocyte (Na(+),K(+))ATPase inhibition induced by zinc is reverted by dithiothreitol
    M G L Ribeiro
    Laboratório de Físico Química Biológica, Instituto de Biofisica Carlos Chagas Filho, UFRJ CCS Ilha do Fundão, Av Brigadeiro Trompowsky, Rio de Janeiro, RJ, Brazil
    Int J Biochem Cell Biol 34:516-24. 2002
    ..electricus main electric organ homogenate. We tried to recover the enzyme activity using dithiothreitol, a reducing agent...
  77. pmc Active site mutations in yeast protein disulfide isomerase cause dithiothreitol sensitivity and a reduced rate of protein folding in the endoplasmic reticulum
    B Holst
    Department of Yeast Genetics, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK 2500 Copenhagen Valby, Denmark
    J Cell Biol 138:1229-38. 1997
    ..Exchanging the wild-type Eug1p C(L/I)HS active site sequences for C(L/I)HC increased the growth rate significantly, however, further highlighting the importance of the oxidizing function for optimal growth...
  78. pmc Inactivation of the SMN complex by oxidative stress
    Lili Wan
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
    Mol Cell 31:244-54. 2008
    ..Thus, the SMN complex is a redox-sensitive assemblyosome and an ROS target, suggesting that it may play a role in oxidative stress pathophysiology, which is associated with many degenerative diseases...
  79. ncbi 20 S proteasome from Saccharomyces cerevisiae is responsive to redox modifications and is S-glutathionylated
    Marilene Demasi
    Departmento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, 277 São Paulo, Sao Paulo 05508 900, Brazil
    J Biol Chem 278:679-85. 2003
    ..The present results indicate that at the physiological level the yeast 20 S proteasome is regulated by its sulfhydryl content, thereby coupling intracellular redox signaling to proteasome-mediated proteolysis...
  80. ncbi Highly conserved cysteines of mouse core 2 beta1,6-N-acetylglucosaminyltransferase I form a network of disulfide bonds and include a thiol that affects enzyme activity
    Ten Yang Yen
    Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132, USA
    J Biol Chem 278:45864-81. 2003
    ..by liquid chromatography/electrospray ionization tandem mass spectrometry in the absence and presence of dithiothreitol. Of nine highly conserved Cys residues, under both conditions, one (Cys217) is a free thiol, and eight are ..
  81. ncbi Single-molecule covalent chemistry with spatially separated reactants
    Tudor Luchian
    Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843 1114, USA
    Angew Chem Int Ed Engl 42:3766-71. 2003
  82. ncbi Familial amyotrophic lateral sclerosis mutants of copper/zinc superoxide dismutase are susceptible to disulfide reduction
    Ashutosh Tiwari
    Department of Neurology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 278:5984-92. 2003
    ..Decreased metal binding site occupancy and exposure to the disulfide-reducing agents dithiothreitol, Tris(2-carboxyethyl)phosphine (TCEP), or reduced glutathione increased the fraction of anomalously migrating ..
  83. pmc Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina
    Ling Yu
    Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, USA
    Biochim Biophys Acta 1773:1473-82. 2007
    ..These findings suggest that PRL-1 is a molecular component of the photoreceptor's response to oxidative stress acting upstream of the glutathione system...
  84. ncbi The Saccharomyces cerevisiae proteome of oxidized protein thiols: contrasted functions for the thioredoxin and glutathione pathways
    Natacha Le Moan
    Laboratoire Stress Oxydants et Cancer, Service de Biologie Moléculaire Systémique, Departement de Biologie Joliot Curie, CEA Saclay, F 91191 Gif sur Yvette Cedex, France
    J Biol Chem 281:10420-30. 2006
    ..Taken together, these results suggest the high selectivity of cytoplasmic protein thiol oxidation...
  85. ncbi Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase
    Gabino F Sanchez-Perez
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Científicas CSIC Universidad Autónoma de Madrid, Arturo Duperier 4, 28029 Madrid, Spain
    J Biol Chem 278:7285-93. 2003
    ..The conditions of refolding, dialysis in the presence of 10 mm dithiothreitol or 10 mm GSH with 1 mm GSSG, allowed the production of both isoforms, the nature of the redox agent ..
  86. ncbi Juxtaposition of the two distal CX3C motifs via intrachain disulfide bonding is essential for the folding of Tim10
    Scott Allen
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:38505-13. 2003
    ..Taken together these data reveal this specific intramolecular disulfide bonding as the crucial mechanism for Tim10 folding and show that the inner cysteine pair has a more prominent role in this process...
  87. ncbi Reduction of pentavalent antimony by trypanothione and formation of a binary and ternary complex of antimony(III) and trypanothione
    Siucheong Yan
    Department of Chemistry and Open Laboratory of Chemical Biology, University of Hong Kong, Pokfulam Road, Hong Kong, PR China
    J Biol Inorg Chem 8:689-97. 2003
    ..Although thermodynamically stable, the complex is kinetically labile and the free and bound forms of thiolates exchange on the (1)H NMR timescale. Such a facile exchange may be crucial for the transport of Sb(III) within parasites...
  88. ncbi Structural and functional determinants in the S5-P region of HCN-encoded pacemaker channels revealed by cysteine-scanning substitutions
    Ka Wing Au
    Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong
    Am J Physiol Cell Physiol 294:C136-44. 2008
    ..Interestingly, the loss of function phenotype of F339C could be rescued by the reducing agent dithiothreitol (DTT)...
  89. pmc Effects of oxidation and reduction on contractile function in skeletal muscle fibres of the rat
    G D Lamb
    Department of Zoology, La Trobe University, Melbourne, Victoria 3086, Australia
    J Physiol 546:149-63. 2003
    ..oxidants hydrogen peroxide (H(2)O(2)) and 2,2'-dithiodipyridine (DTDP), and reductants, glutathione (GSH) and dithiothreitol (DTT), on the properties of the contractile apparatus of rat fast- and slow-twitch skeletal muscle fibres, in ..
  90. ncbi Quantification of protein thiols and dithiols in the picomolar range using sodium borohydride and 4,4'-dithiodipyridine
    Rosa E Hansen
    Institute of Molecular Biology, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Anal Biochem 363:77-82. 2007
    ..acid], DTNB) is the most widely used reagent for quantification of protein thiols, whereas dithiothreitol (DTT) is commonly used for disulfide reduction...
  91. ncbi Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer
    Valérie Noguera-Mazon
    CNRS, Unite Mixte de Recherche, 5180 Sciences Analytiques, Ecole Supérieure Chimie PhysiqueElectronique de Lyon, Domaine Scientifique de la Doua, Universite Claude Bernard, Lyon1, 69622 Villeurbanne, France
    J Biol Chem 281:31736-42. 2006
    ..D-Prx induces the dissociation of the Prx non-covalent homodimer, which can be recovered by reduction with dithiothreitol. This work demonstrates for the first time the existence of a redox-dependent dimer-monomer switch in the Prx ..
  92. pmc A single mutation at Tyr143 of human S-adenosylhomocysteine hydrolase renders the enzyme thermosensitive and affects the oxidation state of bound cofactor nicotinamide-adenine dinucleotide
    Robert Beluzić
    Division of Molecular Medicine, Institute Ruder Boskovic, Bijenicka 54, 10000 Zagreb, Croatia
    Biochem J 400:245-53. 2006
    ..In summary, a single mutation in the AdoHcyase affecting both the oxidation state of bound co-factor NAD and enzyme stability is present in a human with AdoHcyase deficiency...
  93. ncbi Transcriptional activation of dehalorespiration. Identification of redox-active cysteines regulating dimerization and DNA binding
    Stelian M Pop
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 281:26382-90. 2006
    ..When incubated with oxygen or diamide, CprK undergoes inactivation; subsequent treatment with dithiothreitol restores activity...
  94. ncbi The N-terminal domain of PILB from Neisseria meningitidis is a disulfide reductase that can recycle methionine sulfoxide reductases
    Junzhu Wu
    UMR CNRS UHP 7567, Maturation des ARN et Enzymologie Moléculaire, Faculte des Sciences, Bld des Aiguillettes, BP 239, 54506 Vandoeuvre les Nancy, France
    J Biol Chem 280:12344-50. 2005
    ..These results, which show that the N-terminal domain exhibits a disulfide reductase activity and probably has a thioredoxin-fold, are discussed in relation to its possible functional role in Neisseria...
  95. ncbi Inactivation of active and latent transforming growth factor beta by free thiols: potential redox regulation of biological action
    Robert Blakytny
    Department of Cancer Research, Fachklinik Hornheide, D 48157 Münster, Germany
    Int J Biochem Cell Biol 38:1363-73. 2006
    ..g. in burns), as a mechanism protecting from excess TGF-beta activity, as well as in conditions involving redox dysregulation, such as reperfusion injury of the heart, Alzheimer's disease, and cancer...
  96. ncbi Variable reactivity of an engineered cysteine at position 338 in cystic fibrosis transmembrane conductance regulator reflects different chemical states of the thiol
    Xuehong Liu
    Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Biol Chem 281:8275-85. 2006
    ..One of the altered states displays reactivity toward thiols like dithiothreitol and 2-mercaptoethanol as well as reagents: mixed disulfides (methanethiosulfonate reagents: MTSET+, MTSES-) ..
  97. ncbi Microsomal UDP-glucuronyltransferase in rat liver: oxidative activation
    María Eugenia Letelier
    Laboratory of Pharmacology, Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile
    Basic Clin Pharmacol Toxicol 96:480-6. 2005
    ..This activation was prevented or reversed by the reducing agents glutathione, cysteine or dithiothreitol when it was induced by the Fe(3+)/ascorbate...
  98. ncbi Characterization of the redox and metal binding activity of BsSco, a protein implicated in the assembly of cytochrome c oxidase
    Iveta Imriskova-Sosova
    Department of Biochemistry, Queen s University, Kingston, Ontario, K7L 3N6 Canada
    Biochemistry 44:16949-56. 2005
    ..Cobalt(II) exhibits weak binding, whereas Ni(II) and Zn(II) do not appear to bind. The high-affinity binding of metals by BsSco is triggered by its redox state, and this property could be important for its function in vivo...
  99. ncbi Reactivities of quinone-free DsbB from Escherichia coli
    Kenji Inaba
    Institute for Virus Research, Kyoto University, and CREST of Japan Science and Technology Agency, Kyoto 606 8507, Japan
    J Biol Chem 280:33035-44. 2005
    ..redox potentials of cysteine pairs of quinone-free DsbB are lower than that of DsbA, as far as determined in dithiothreitol redox buffer...
  100. ncbi Interactions of sodium selenite, glutathione, arsenic species, and omega class human glutathione transferase
    Robert A Zakharyan
    Department of Molecular and Cellular Biology, The University of Arizona, Tucson, Arizona 85721 0106, USA
    Chem Res Toxicol 18:1287-95. 2005
    ..The inhibition of the MMA(V) reducting activity of hGSTO1-1 by selenite was reversed by 1 mM DL-dithiothreitol (DTT) but not by reduced glutathione (GSH), which is a required substrate for the enzyme...
  101. ncbi Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasis
    Noriyuki Nagahara
    Department of Environmental Medicine, Nippon Medical School, Tokyo 113 8602, Japan
    J Biol Chem 280:34569-76. 2005
    ..5 microM and k(i) = 2.5 min(-1), K(i) = 178.6 microM, respectively). The activity was completely restored by dithiothreitol or thioredoxin with a reducing system containing thioredoxin reductase and NADPH, but glutathione did not ..

Research Grants66

  1. Control of virulence in Neisseria meningitidis
    Yih Ling Tzeng; Fiscal Year: 2012
    ..invasion, in regulation of capsule expression as well as the initial identification of a host signal mimic: dithiothreitol (DTT) stress...
  2. EFFECT OF AGING ON INTERORGAN GLUTATHIONE HOMEOSTASIS
    Murad Ookhtens; Fiscal Year: 1999
    ..2) Perfusions of isolated livers and incubations of freshly-isolated hepatocytes with dithiothreitol (DTT), N- acetylcysteine (NAC) and dimercaprol, in addition to GSH, CYSH and CYSS. 6...
  3. DEPENDENCE OF P53 ON THIOL MAINTENANCE PROTEINS
    Gary Merrill; Fiscal Year: 2001
    ..determine whether p53 modifications that result in Trr1-independent transcriptional activity in vivo result in dithiothreitol-independent DNA binding activity in vitro...
  4. DISULFIDE SURFACE STRUCTURE IN LEGIONELLA PATHOGENESIS
    Paul Hoffman; Fiscal Year: 1990
    ..Virulent (V), but not avirulent (AV) isogenic strains release proteins when treated with dithiothreitol suggesting that differences in MOMP structure might correlate with virulence...
  5. COMPLEX CARBOHYDRATES
    LAWRENCE MWASI; Fiscal Year: 2002
    ..The enzymatic activity could be kept fairly stable in 20% glycerol containing o.5 mM dithiothreitol. The enzyme showed a pH optimum of about 8.0 in 50 mM Tris buffer...
  6. Proteomics of Complex 1 Inhibition in GSH-Depleted Cells
    Bradford Gibson; Fiscal Year: 2004
    ..activity appears to be a result of cysteine oxidation which is reversible by the thiol-reducing agent dithiothreitol. These results suggest that early observed GSH losses in PD may be directly responsible for the noted ..
  7. METALLOPROTEINASE IN HORMONE DEPENDENT BREAST CANCER
    Y Shi; Fiscal Year: 1999
    ..So far, the known inhibitors of the enzyme are EDTA, dithiothreitol, and leuptin...
  8. PROPERTIES OF THE HIGH-AFFINITY INSULIN RECEPTOR
    Michael Czech; Fiscal Year: 1980
    ..It exists as a disulfide linked complex (300,000 daltons) which is reduced to a 125,000 monomer by dithiothreitol. Development of a selective precipitation procedure to rapidly and specifically resolve the detergent-..
  9. CANCER CELL-DERIVED PA OF UNUSUALLY LARGE SIZE
    Shashikumar Harvey; Fiscal Year: 1990
    ..2000,000 dalton peptide chain. Reduction with increasing concentrations of dithiothreitol in the presence and absence of SDS will be used to verify this interpretation...
  10. MECHANISMS OF RADIOPROTECTION & TOXICITY OF SH COMPOUNDS
    KATHRYN HELD; Fiscal Year: 1992
    ..The thiols to be used are dithiothreitol, glutathione, and cysteamine and its derivatives, including WR-1065...
  11. MUTATIONAL ANALYSIS OF ANGIOTENSIN II RECEPTORS
    Daniel Yee; Fiscal Year: 2001
    ..of disulfide bridges and as a source for the differing sensitivities of the two subtypes to the reducing agent dithiothreitol; (iv) identifying the structural elements that are responsible for AT2 subtype selective binding; and (v) ..
  12. Molecular Neurobiology of Drug Abuse
    DONALD KUHN; Fiscal Year: 2006
    ..Kuhn will remain extensively engaged in scientific mentoring activities. ..
  13. NEUROSCIENCE TRAINING IN DRUG ABUSE
    DONALD KUHN; Fiscal Year: 2004
    ..abstract_text> ..
  14. PP2A-Like Constitutive ERK/MAPK Phosphatase in Brain
    Timothy Foley; Fiscal Year: 2003
    ....
  15. Methamphetamine Neurotoxicity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2009
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  16. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2002
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
  17. PKC SIGNALING AND THE TREATMENT OF BIPOLAR DISORDER
    DONALD KUHN; Fiscal Year: 2002
    ....
  18. Receptor Affects Fungal Mucosal Colonization in HIV
    Mary Ann Jabra Rizk; Fiscal Year: 2005
    ..dubliniensis to be preferentially selected. ..
  19. MOLECULAR BASIS OF VIRULENCE FACTORS OF ORAL FUNGAL SP.
    Mary Ann Jabra Rizk; Fiscal Year: 2006
    ..Long range plans include using the mutants generated to study Candida mannoprotein-specific host immunomodulation, such as stimulation of cytokines and chemokines. ..
  20. Neutralizing Compounds for Viral Hemorrhagic Fever
    Richard Larson; Fiscal Year: 2005
    ....
  21. FUNCTIONAL ARCHITECTURE OF RETINAL ROD CNG ION CHANNELS
    SHARONA GORDON; Fiscal Year: 2006
    ..Through these experiments we expect to gain significant insight into how the cGMP-gated ion channels function during visual transduction. ..
  22. MOLECULAR MECHANISMS OF LUNG SURFACTANT SECRETION
    Lin Liu; Fiscal Year: 2007
    ..Accomplishing the goals of this proposal will give a valuable direction to the therapy of pulmonary diseases such as neonatal respiratory distress syndrome. ..
  23. DEVELOPMENT OF AN ORAL CARRIER
    Lance L Simpson; Fiscal Year: 2010
    ..To demonstrate an absence of toxicity of the modified carrier and the modified carrier~tetanus antigen construct If successful, this work should demonstrate that a mucosal tetanus vaccine is worthy of evaluation as a clinical product. ..
  24. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..Pilot data from this R03 small grants program research will be used to apply for funding through the R01 mechanism. [unreadable] [unreadable] [unreadable]..
  25. Nitric oxide and mitochondrial biogenesis in sepsis
    Claude A Piantadosi; Fiscal Year: 2010
    ..abstract_text> ..
  26. Aminoglycoside uptake and clearance in mammalian cells
    Takatoshi Karasawa; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  27. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  28. Regulation of Oxidative Stress in the mdx Diaphragm
    JOHN LAWLER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  29. Proteasome function in the aging retina
    Deborah A Ferrington; Fiscal Year: 2010
    ..Results from these experiments will help verify that immunoproteasome is an essential component of the retinal stress response and plays a fundamental role in maintaining retinal health. ..
  30. The Effect of Alcohol on NADPH oxidase structure in pancreatic stellate cells
    Kym Faull; Fiscal Year: 2008
    ..Successful completion of the experiments described in this proposal are essential for the development of targeted therapeutic agents for treatment of alcohol related pancreatitis. [unreadable] [unreadable] [unreadable]..
  31. Redox Regulation of Cardiac Electrical Remodeling
    GEORGE ROZANSKI; Fiscal Year: 2009
    ..PHS 398 (Rev. 09/04) Page 2 Principal Investigator/Program Director (Last, first, middle): Rozanski, George, J ..
  32. Collaborative Development of a Vaccine against Cutaneous and Visceral Leishmanias
    MARY EDYTHE WILSON; Fiscal Year: 2010
    ..Through a coordinated approach in the three laboratories, we will systematically perform pre-clinical testing against several Leishmania species, with the goal of developing a pan-leishmania protective vaccine. ..
  33. Mechanisms of Alveolar Fluid Transport
    Lin Liu; Fiscal Year: 2009
    ..An understanding of the mechanisms of fluid transport in the lung will provide valuable insights and directions toward therapeutic intervention in fetal lung diseases and the resolution of pulmonary edema. ..
  34. Assay for Formylpeptide Receptor Family Ligands(RMI)
    Bruce Edwards; Fiscal Year: 2005
    ..We expect that such compounds will prove to be important tools with which to advance the understanding and therapy of inflammation-associated diseases. ..
  35. Photodynamic therapy of oral candidiasis
    CONSTANTINE HAIDARIS; Fiscal Year: 2005
    ..2b. To demonstrate proof of principle of PDT in the treatment of C. albicans infection in the oral cavity of rats in vivo. ..
  36. GP63 EXPRESSION IN LEISHMANIA CHAGASI
    Mary Wilson; Fiscal Year: 2001
    ..They will also provide a model for differential expression of tandemly linked genes in this group of protozoa. ..
  37. PURCHASE OF MASS SPECTROMETRIC INSTRUMENTATION
    Kym Faull; Fiscal Year: 2004
    ..Descriptions of the research of the seven major users of the instrument are provided. ..
  38. NITRIC OXIDE PRODUCTION BY ANG II IN ENDOTHELIUM
    Susan Olson; Fiscal Year: 2004
    ..These studies are of significant medical importance to heart and pulmonary disease, which involve pathological changes in blood pressure. ..
  39. RECEPTOR MEDIATED OXIDATIVE STRESS
    HOWARD SHERTZER; Fiscal Year: 2003
    ..These studies will improve our understanding of how PHAHs such as TCDD generate oxidative stress and mutagenesis, leading to better models for risk assessment and novel strategies for intervention in PHAH-mediated toxicity. ..
  40. Cytoskeletal organization in apicomplexan parasites
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 research will be used to apply for funding through the RO1 mechanism. ..
  41. Prevention of Recombinant Protein Aggregation in E.coli
    Shaorong Chong; Fiscal Year: 2005
    ..As a long-term goal, this project aims to provide insights into the mechanism of the aggregation by these disease-related proteins and the means to prevent aggregation. ..
  42. Immunogenetics of Leishmania chagasi Infection
    Mary Wilson; Fiscal Year: 2005
    ..chagasi infection, and to identify specific alleles that influence human immune responses and thereby modify the course of disease. ..
  43. OXIDATIVE DNA DAMAGE AND REPAIR IN CNS CELLS
    Mark Kelley; Fiscal Year: 2003
    ..abstract_text> ..
  44. The Major Surface Protease of Trypanosomatid Protozoa
    Mary Wilson; Fiscal Year: 2003
    ..chagasi whose coding sequences predict they lack the usual GPI anchor, and to contrast the amino acid sequences that regulate the intracellular trafficking of these unusual MSPs with the MSPs that possess a GPI membrane anchor. ..
  45. Molecular determinants of ACTH receptor for ligand binding
    Yingkui Yang; Fiscal Year: 2006
    ..The information will serve to establish a foundation on which to build a comprehensive understanding of the signaling events that regulate ACTH mediated adrenal function in physiologic and diseased states. ..
  46. Lung cancer chemoprevention and tocopheryl succinate
    Richard Wright; Fiscal Year: 2005
    ..The overall goal of this work is that it may contribute to an eventual reduction in the incidence or severity of human lung cancers. ..
  47. PHybrid Quadrupole/Orthogonal Time-of-Flight Mass Spec
    Kym Faull; Fiscal Year: 2002
    ..Reflecting this diversity of use, descriptions of the research of the major users of the instrument are provided...
  48. Functional Organization of the Myelin Membrane
    Christopher Taylor; Fiscal Year: 2003
    ..in forming rafts in myelin, 3) provide a more complete understanding of the organization of proteins and lipids in myelin as well as identify potential targets for the therapeutic intervention in many myelin related disorders/diseases ..
  49. Structure of the Glycine Receptor's Alcohol Binding Site
    INGRID LOBO; Fiscal Year: 2003
    ..abstract_text> ..
  50. Role of the Unfolded Protein Response in Type 2 Diabetes
    FEROZ PAPA; Fiscal Year: 2008
    ..The UCSF Department of Medicine is fully committed to Dr. Papa's career development, and will make all necessary resources available to facilitate the success of the project. ..
  51. Discriminative Stimulus Effects of Alcohol in Mice
    Wouter Koek; Fiscal Year: 2007
    ..This future research will increase our understanding of risk factors for alcoholism and drug abuse and will help the development of effective approaches to prevention and treatment. [unreadable] [unreadable] [unreadable]..
  52. Biophysics of CNS Connexins
    VYTAUTAS VERSELIS; Fiscal Year: 2006
    ..abstract_text> ..
  53. Proteomics of altered protein in macular degeneration
    DEBORAH FERRINGTON; Fiscal Year: 2006
    ..Identification of proteins that are uniquely altered will help provide valuable insight into the mechanism of AMD. ..
  54. Therapeutic/Mechanistic Role of Ape1 in Germ Cell Tumors
    Mark Kelley; Fiscal Year: 2007
    ..If any mutants are shown to sensitize the GCT cell lines to chemo-/IR agents, as expected, this will set the stage for future gene therapy approaches to sensitize GCT's to therapy. ..
  55. Mechanisms of Alveolar Epithelial Cell Differentiation
    Lin Liu; Fiscal Year: 2006
    ..abstract_text> ..
  56. "Ligand Independent" endocrine disruption by pesticides
    Patrick Wong; Fiscal Year: 2005
    ..abstract_text> ..
  57. Agmatine Mediated Arrest of Proliferation
    Joseph Satriano; Fiscal Year: 2003
    ..UCSD has established itself as a leading academic institution and thus provides an excellent environment for the exchange of ideas and techniques essential for the growth of the PI's career. ..
  58. ALDOSTERONE, HYPERTENSION AND THE BRAIN
    Elise Gomez Sanchez; Fiscal Year: 2003
    ..4) Study of the metabolism of aldosterone and corticosterone and the regulation of the synthesis of low polarity derivatives in the brain, including the identification of the sites of formation of aldosterone acyl esters. ..
  59. CYTOMIMETIC ORGANIC CHEMISTRY
    FREDRIC MENGER; Fiscal Year: 2007
    ....
  60. Probing the pore of CFTR using chemical modification
    Xuehong Liu; Fiscal Year: 2003
    ..mechanism to explain how HFS type diets cause insulin resistance. and hypertension, and how a LFCC diet, antioxidant therapy or exercise training aid in the control of insulin resistance and hypertension. ..
  61. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
  62. A tyrosinase-targeted drug design for the treatment of melanoma
    Majid Moridani; Fiscal Year: 2007
    ..In this work we will be developing novel phenol based drugs for treatment of melanoma. [unreadable] [unreadable] [unreadable]..
  63. Redox Control of Radiation Response
    John Biaglow; Fiscal Year: 2006
    ..Ultimately, we hope to develop specific strategies for modifying thiol redox homeostasis in vivo, to make human tumors more sensitive to ionizing radiation. ..
  64. MOLECULAR MECHANISMS OF PACEMAKER CHANNEL FUNCTION
    Michael Sanguinetti; Fiscal Year: 2008
    ..These studies promise to further our understanding of the biological oscillator that controls heart rhythm. ..