sugar phosphates

Summary

Top Publications

  1. ncbi E. coli MEP synthase: steady-state kinetic analysis and substrate binding
    Andrew T Koppisch
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 41:236-43. 2002
  2. ncbi Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran
    Ho Min Kim
    Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejon, Korea 305 701
    Cell 130:906-17. 2007
  3. ncbi Determination of trehalose-6-phosphate in Arabidopsis seedlings by successive extractions followed by anion exchange chromatography-mass spectrometry
    Thierry L Delatte
    Department of Biomedical Analysis, Utrecht University, 3584 CA Utrecht, The Netherlands
    Anal Biochem 389:12-7. 2009
  4. pmc Systematic phenome analysis of Escherichia coli multiple-knockout mutants reveals hidden reactions in central carbon metabolism
    Kenji Nakahigashi
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    Mol Syst Biol 5:306. 2009
  5. ncbi Trehalose-6-phosphate synthase 1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation
    Peter J Eastmond
    Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York, YO10 5DD, UK
    Plant J 29:225-35. 2002
  6. ncbi Unravelling the regulatory mechanisms that modulate the MEP pathway in higher plants
    Elizabeth Cordoba
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Col Chamilpa, Cuernavaca, Morelos 62250, Mexico
    J Exp Bot 60:2933-43. 2009
  7. ncbi Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice
    Atsushi Okada
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo, Japan
    Plant Mol Biol 65:177-87. 2007
  8. ncbi The Arabidopsis csb3 mutant reveals a regulatory link between salicylic acid-mediated disease resistance and the methyl-erythritol 4-phosphate pathway
    M José Gil
    Instituto de Biología Molecular y Celular de Plantas IBMCP, UPV CSIC, Camino de Vera, S N, 46022 Valencia, Spain
    Plant J 44:155-66. 2005
  9. pmc Last step in the conversion of trehalose to glycogen: a mycobacterial enzyme that transfers maltose from maltose 1-phosphate to glycogen
    Alan D Elbein
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Biol Chem 285:9803-12. 2010
  10. ncbi Spatial distribution and hormonal regulation of gene products from methyl erythritol phosphate and monoterpene-secoiridoid pathways in Catharanthus roseus
    Audrey Oudin
    EA 2106 Biomolécules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Universite Francois Rabelais de Tours, Tours, France
    Plant Mol Biol 65:13-30. 2007

Research Grants

Detail Information

Publications214 found, 100 shown here

  1. ncbi E. coli MEP synthase: steady-state kinetic analysis and substrate binding
    Andrew T Koppisch
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 41:236-43. 2002
    ..Fosmidomycin was an uncompetitive inhibitor against NADPH and gave a pattern representative of slow, tight-binding competitive inhibition against DXP. These results are consistent with an ordered mechanism where NADPH binds before DXP...
  2. ncbi Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran
    Ho Min Kim
    Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejon, Korea 305 701
    Cell 130:906-17. 2007
    ..The interaction with Eritoran is mediated by a hydrophobic internal pocket in MD-2. Based on structural analysis and mutagenesis experiments on MD-2 and TLR4, we propose a model of TLR4-MD-2 dimerization induced by LPS...
  3. ncbi Determination of trehalose-6-phosphate in Arabidopsis seedlings by successive extractions followed by anion exchange chromatography-mass spectrometry
    Thierry L Delatte
    Department of Biomedical Analysis, Utrecht University, 3584 CA Utrecht, The Netherlands
    Anal Biochem 389:12-7. 2009
    ..Results reveal that T6P accumulation differed substantially in the four mutant lines and wild type (WT). It is concluded that the mutants circumvent the growth arrest observed in WT seedlings on 100mM trehalose by different mechanisms...
  4. pmc Systematic phenome analysis of Escherichia coli multiple-knockout mutants reveals hidden reactions in central carbon metabolism
    Kenji Nakahigashi
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    Mol Syst Biol 5:306. 2009
    ..The emergence of an alternative pathway not requiring any changes in gene expression, but rather relying on the accumulation of an intermediate metabolite may be a novel mechanism mediating the robustness of these metabolic networks...
  5. ncbi Trehalose-6-phosphate synthase 1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation
    Peter J Eastmond
    Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York, YO10 5DD, UK
    Plant J 29:225-35. 2002
    ..Our data establish for the first time that an enzyme of trehalose metabolism is essential in plants and is implicated in the regulation of sugar metabolism/embryo development via a different mechanism to that reported in S. cerevisiae...
  6. ncbi Unravelling the regulatory mechanisms that modulate the MEP pathway in higher plants
    Elizabeth Cordoba
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Col Chamilpa, Cuernavaca, Morelos 62250, Mexico
    J Exp Bot 60:2933-43. 2009
    ..The evidence accumulated suggests that this regulation might link the activity of this pathway with the plant's physiological conditions and the metabolic demand for the final products of this route...
  7. ncbi Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice
    Atsushi Okada
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo, Japan
    Plant Mol Biol 65:177-87. 2007
    ..These results suggest that activation of the MEP pathway is required to supply sufficient terpenoid precursors for the production of phytoalexins in infected rice plants...
  8. ncbi The Arabidopsis csb3 mutant reveals a regulatory link between salicylic acid-mediated disease resistance and the methyl-erythritol 4-phosphate pathway
    M José Gil
    Instituto de Biología Molecular y Celular de Plantas IBMCP, UPV CSIC, Camino de Vera, S N, 46022 Valencia, Spain
    Plant J 44:155-66. 2005
    ....
  9. pmc Last step in the conversion of trehalose to glycogen: a mycobacterial enzyme that transfers maltose from maltose 1-phosphate to glycogen
    Alan D Elbein
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Biol Chem 285:9803-12. 2010
    ....
  10. ncbi Spatial distribution and hormonal regulation of gene products from methyl erythritol phosphate and monoterpene-secoiridoid pathways in Catharanthus roseus
    Audrey Oudin
    EA 2106 Biomolécules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Universite Francois Rabelais de Tours, Tours, France
    Plant Mol Biol 65:13-30. 2007
    ..roseus cells, our results support that intercellular translocation likely plays an important role during monoterpene-secoiridoid assembly...
  11. ncbi Arbuscular mycorrhizal fungi induce the non-mevalonate methylerythritol phosphate pathway of isoprenoid biosynthesis correlated with accumulation of the 'yellow pigment' and other apocarotenoids
    M H Walter
    Leibniz Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Weinberg 3, D 06120 Halle Saale, Germany
    Plant J 21:571-8. 2000
    ..This is the first report demonstrating (i) that the plastidic MEP pathway is active in plant roots and (ii) that it can be induced by a fungus...
  12. pmc Distinct light-mediated pathways regulate the biosynthesis and exchange of isoprenoid precursors during Arabidopsis seedling development
    Manuel Rodriguez-Concepcion
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, 08028 Barcelona, Spain
    Plant Cell 16:144-56. 2004
    ....
  13. pmc Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5-phosphate synthase of the 2-C-methyl-D-erythritol-4-phosphate pathway in Arabidopsis
    J M Estevez
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 2001 Chamilpa, Apdo Postal 510 3 Cuernavaca Morelos 62271, Mexico
    Plant Physiol 124:95-104. 2000
    ..Microscopic analysis also shows a pleiotropic effect of the CLA1 gene mutation in mesophyll tissue formation...
  14. ncbi The plastidial MEP pathway: unified nomenclature and resources
    Michael A Phillips
    Departament de Genètica Molecular Vegetal, Centre de Recerca en Agrogenòmica CRAG CSIC IRTA UAB, C Jordi Girona 18, 08034 Barcelona, Spain
    Trends Plant Sci 13:619-23. 2008
    ..Here, we propose a reorganization of the various naming systems with the aim of facilitating the dissemination and sharing of genetic resources and tools central to plant isoprenoid research...
  15. ncbi fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
    Kia Joo Puan
    Division of Rheumatology, Department of Internal Medicine and the Interdisciplinary Group in Immunology, University of Iowa, EMRB 340F, Iowa City, IA 52242, USA
    FEBS Lett 579:3802-6. 2005
    ..Thus, the essential role for flavodoxin I in E. coli is in the MEP pathway for isoprenoid biosynthesis...
  16. pmc A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis
    S Takahashi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 95:9879-84. 1998
    ..coli...
  17. ncbi The MEP pathway: a new target for the development of herbicides, antibiotics and antimalarial drugs
    M Rodriguez-Concepcion
    Department de Bioquimica i Biologia Molecular, Facultat de Quimica, Universidad de Barcelona, Barcelona, Spain
    Curr Pharm Des 10:2391-400. 2004
    ..Current efforts should eventually contribute to an effective drug designed to fight against microbial pathogens that show resistance to currently available agents...
  18. ncbi Sugar signals and molecular networks controlling plant growth
    Sjef Smeekens
    Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Curr Opin Plant Biol 13:274-9. 2010
    ..It is crucial that these systems interact closely in regulating growth and in several cases crosstalk has been demonstrated. Importantly, these nutrient controlled systems must interact with other growth regulatory pathways...
  19. ncbi The herbicide ketoclomazone inhibits 1-deoxy-D-xylulose 5-phosphate synthase in the 2-C-methyl-D-erythritol 4-phosphate pathway and shows antibacterial activity against Haemophilus influenzae
    Yukiko Matsue
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    J Antibiot (Tokyo) 63:583-8. 2010
    ..These data reveal the inhibitory mechanism of ketoclomazone on DXP synthase...
  20. ncbi Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency
    Tamas Kardon
    Laboratory of Physiological Chemistry, de Duve Institute, Universite Catholique de Louvain, Ave Hippocrate 75, B 1200 Brussels, Belgium
    FEBS Lett 582:3330-4. 2008
    ..Cleavage of the latter by aldolase B would lead to the formation of erythrose, which would then be reduced to erythritol...
  21. ncbi Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli
    R J Kadner
    Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908
    J Gen Microbiol 138:2007-14. 1992
    The Escherichia coli uhp T gene encodes an active transport system for sugar phosphates. When the uhp T gene was carried on a multicopy plasmid, amplified levels of transport activity occurred, and growth of these strains was inhibited ..
  22. pmc Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics
    Manuel Rodriguez-Concepcion
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Martí i Franquès 1 7, 08028 Barcelona, Spain
    Plant Physiol 130:1079-89. 2002
  23. pmc Divergent regulation of terpenoid metabolism in the trichomes of wild and cultivated tomato species
    Katrin Besser
    Center for Novel Agricultural Products, Department of Biology, University of York, York YO10 5YW, United Kingdom
    Plant Physiol 149:499-514. 2009
    ..However, highly trichome-expressed sesquiterpene cyclase-like enzymes were ruled out as being involved in the production of major LA1777 sesquiterpenes...
  24. ncbi Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system
    Carin K Vanderpool
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 54:1076-89. 2004
    ..Decreased ptsG mRNA results in decreased production of glucose transport machinery, thus limiting further accumulation of glucose phosphate...
  25. pmc The nonmevalonate pathway supports both monoterpene and sesquiterpene formation in snapdragon flowers
    Natalia Dudareva
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 102:933-8. 2005
    ..The trafficking of IPP occurs unidirectionally from the plastids to cytosol. The MEP pathway operates in a rhythmic manner controlled by the circadian clock, which determines the rhythmicity of terpenoid emission...
  26. ncbi A systems biology investigation of the MEP/terpenoid and shikimate/phenylpropanoid pathways points to multiple levels of metabolic control in sweet basil glandular trichomes
    Zhengzhi Xie
    Department of Plant Sciences and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA
    Plant J 54:349-61. 2008
    ..Additionally, precursors for different classes of terpenoids, including mono- and sesquiterpenoids, appear to be almost exclusively supplied by the MEP pathway, and not the mevalonate pathway, in basil glandular trichomes...
  27. ncbi Co-expression of three MEP pathway genes and geraniol 10-hydroxylase in internal phloem parenchyma of Catharanthus roseus implicates multicellular translocation of intermediates during the biosynthesis of monoterpene indole alkaloids and isoprenoid-derive
    Vincent Burlat
    EA 2106, Plant Biocompounds and Biotechnology, UFR des Sciences et Techniques, Universite de Tours, 37200 Tours, France
    Plant J 38:131-41. 2004
    ..Similarly, the translocation of intermediates from the phloem parenchyma is probably also required during the biosynthesis of hormones and photosynthetic primary metabolites derived from the MEP pathway...
  28. ncbi Supply of precursors for carotenoid biosynthesis in plants
    Manuel Rodriguez-Concepcion
    Centre for Research in Agricultural Genomics CRAG CSIC IRTA UAB, Jordi Girona 18, 08034 Barcelona, Spain
    Arch Biochem Biophys 504:118-22. 2010
    ....
  29. ncbi Trehalose 6-phosphate: a signal of sucrose status
    Matthew J Paul
    Centre for Crop Genetic Improvement, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
    Biochem J 412:e1-2. 2008
    ..Using this technology, the authors showed large changes in T6P content that reflect tissue sucrose status. Overall, this elegant work makes an important contribution towards our understanding of the function of T6P in plants...
  30. ncbi Mevalonic acid partially restores chloroplast and etioplast development in Arabidopsis lacking the non-mevalonate pathway
    Noriko Nagata
    Laboratory for Biochemical Resources, Plant Science Center, RIKEN, Wako, Saitama 351 0198, Japan
    Planta 216:345-50. 2002
    ..These results suggest that mevalonic acid-derived products contribute to the formation of functional plastidic isoprenoids, such as the chlorophylls and carotenoids required for plastid development...
  31. pmc Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of dolichols in plants
    Karolina Skorupinska-Tudek
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland
    J Biol Chem 283:21024-35. 2008
    ..This study also illustrates an innovative application of mass spectrometry for qualitative and quantitative evaluation of the contribution of individual metabolic pathways to the biosynthesis of natural products...
  32. ncbi An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana
    B Leyman
    Vlaams Interuniversitair Instituut voor Biotechnologie VIB, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Flanders, Belgium
    Trends Plant Sci 6:510-3. 2001
    ..Incompatibility between high trehalose levels and chaperone-assisted protein folding might be a reason why plants have evolved to accumulate some alternative stress-protection compounds to trehalose...
  33. ncbi LytB protein catalyzes the terminal step of the 2-C-methyl-D-erythritol-4-phosphate pathway of isoprenoid biosynthesis
    Boran Altincicek
    Jomaa Pharmaka GmbH, Frankfurter Strasse 50, 35392, Giessen, Germany
    FEBS Lett 532:437-40. 2002
    ..The maximal specific activity of 6.6+/-0.3 micromol x min(-1) x mg(-1) protein was determined at pH 7.5 and 60 degrees C. The k(cat) value of the LytB protein was 3.7+/-0.2 s(-1) and the K(m) value for HMBPP was 590+/-60 microM...
  34. ncbi Inhibition of Toll-like receptor 4 with eritoran attenuates myocardial ischemia-reperfusion injury
    Akira Shimamoto
    Department of Thoracic and Cardiovascular Surgery, Mie University Graduate School of Medicine, 2 174, Edobashi, Tsu, Mie 514 8507, Japan
    Circulation 114:I270-4. 2006
    ..To investigate the ligand-activating TLR4 during MI/R injury, we evaluated the effect of eritoran, a specific TLR4 antagonist, on MI/R injury, with the goal of defining better therapeutic options for MI/R injury...
  35. pmc Inhibition of SNF1-related protein kinase1 activity and regulation of metabolic pathways by trehalose-6-phosphate
    Yuhua Zhang
    Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom
    Plant Physiol 149:1860-71. 2009
    ..T6P, but not other sugars and sugar phosphates, inhibited SnRK1 in Arabidopsis (Arabidopsis thaliana) seedling extracts strongly (50%) at low concentrations ..
  36. pmc Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate
    S Herz
    Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    Proc Natl Acad Sci U S A 97:2486-90. 2000
    ..This compound does not serve as substrate for the formation of carotenoids by isolated chromoplasts and is assumed to be an in vitro product without metabolic relevance...
  37. pmc A mutant impaired in the production of plastome-encoded proteins uncovers a mechanism for the homeostasis of isoprenoid biosynthetic enzymes in Arabidopsis plastids
    Ursula Flores-Pérez
    Departament de Genètica Molecular de Plantes, Centre for Research on Agricultural Genomics, 08034 Barcelona, Spain
    Plant Cell 20:1303-15. 2008
    ....
  38. ncbi Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids
    J Schwender
    Botanisches Institut, Lehrstuhl II, , Germany
    Planta 212:416-23. 2001
    ....
  39. ncbi Isolation and characterization of maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes missouriensis
    Birgit Niehues
    Westfalische Wilhelms Universitat, Institut fur Mikrobiologie, Corrensstrasse 3, 48149, Munster, Germany
    Arch Microbiol 180:233-9. 2003
    ..Tryptic peptides of the enzyme were sequenced, and the sequences of these peptides will allow construction of degenerate primers to identify the gene coding for this unique kinase...
  40. pmc CHLOROPLAST BIOGENESIS genes act cell and noncell autonomously in early chloroplast development
    María de la Luz Gutiérrez-Nava
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos 62271, Mexico
    Plant Physiol 135:471-82. 2004
    ..The isolation and characterization of clb mutants represents the first systematic study of genes required for early chloroplast development in Arabidopsis...
  41. ncbi Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 54:1063-75. 2004
    ..In addition, we show that PNPase and RhlB within the degradosome cooperate to eliminate short degradation intermediates of ptsG mRNA...
  42. ncbi Knock-down of the MEP pathway isogene 1-deoxy-D-xylulose 5-phosphate synthase 2 inhibits formation of arbuscular mycorrhiza-induced apocarotenoids, and abolishes normal expression of mycorrhiza-specific plant marker genes
    Daniela S Floss
    Abteilung Sekundärstoffwechsel, Leibniz Institut für Pflanzenbiochemie IPB, Weinberg 3, D 06120 Halle Saale, Germany
    Plant J 56:86-100. 2008
    ..They further validate the concept of a distinct role for DXS2 in secondary metabolism, and offer a novel tool to selectively manipulate the levels of secondary isoprenoids by targeting their precursor supply...
  43. ncbi Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein
    Murielle Wolff
    Universite Louis Pasteur, UMR CNRS 7123, Institut Le Bel, Strasbourg, France
    FEBS Lett 541:115-20. 2003
    ..Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical...
  44. ncbi Cross-talk between the cytosolic mevalonate and the plastidial methylerythritol phosphate pathways in tobacco bright yellow-2 cells
    Andrea Hemmerlin
    CNRS, UPR 2357, Institut de Biologie Moleculaire des Plantes, 28 rue Goethe, 67083 Strasbourg Cedex, France
    J Biol Chem 278:26666-76. 2003
    ..Best rates of incorporation of exogenous stably labeled precursors were observed in the presence of both inhibitors, thereby avoiding internal isotope dilution...
  45. ncbi Sugar perception and signaling--an update
    Johannes Hanson
    Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Curr Opin Plant Biol 12:562-7. 2009
    ..bZIP transcription factors were found to mediate effects of sugar signaling on gene expression and metabolite content...
  46. ncbi Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of (13)C-labelled C(4) to C(7) sugar phosphates
    J K MacLeod
    Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    J Mass Spectrom 36:500-8. 2001
    ..The results confirm that GC/MS of the alkoxime-TMS derivatives is a viable method for measuring (13)C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with (13)CO(2).
  47. ncbi Quantitative analysis of intracellular sugar phosphates and sugar nucleotides in encapsulated streptococci using HPAEC-PAD
    Esteban Marcellin
    Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD, Australia
    Biotechnol J 4:58-63. 2009
    ..Quantification of sugar phosphates and nucleotide sugars was performed using high-performance anion exchange chromatography combined with pulsed ..
  48. pmc Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol
    F Rohdich
    Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    Proc Natl Acad Sci U S A 96:11758-63. 1999
    ..A gene from Arabidopsis thaliana with similarity to ygbP carries a putative plastid import sequence, which is well in line with the assumed localization of the deoxyxylulose pathway in the plastid compartment of plants...
  49. ncbi Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric detection
    Ji Seon Jeong
    Department of Oriental Pharmaceutical Sciences, Kyung Hee University, Dongdaemoon Gu Hoegi dong 1, Seoul, South Korea
    J Chromatogr A 1164:167-73. 2007
    We have developed an improved analytical method for the determination of sugar phosphates using sodium carbonate (Na(2)CO(3)) for high-performance anion-exchange chromatography-pulsed amperometric detection...
  50. pmc A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT
    Ana Nobre
    Centro de Neurociencias e Biologia Celular, Departamento de Zoologia, Universidade de Coimbra, 3004 517 Coimbra, Portugal
    J Bacteriol 190:7939-46. 2008
    ..xylanophilus, a representative of an ancient lineage of the actinobacteria, and discuss a possible scenario for the exceptional occurrence of treT in this extremophilic bacterium...
  51. pmc Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4-phosphate pathway
    Arturo Guevara-García
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Morelos 62271, Mexico
    Plant Cell 17:628-43. 2005
    ..Our results show that the first enzyme of the pathway, 1-deoxy-d-xylulose 5-phosphate synthase, is feedback regulated in response to the interruption of the flow of metabolites through the MEP pathway...
  52. ncbi Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein
    Myriam Seemann
    Universite Louis Pasteur, UMR 7123 CNRS, Institut Le Bel 4 rue Blaise Pascal, 67 070 Strasbourg Cedex, France
    Angew Chem Int Ed Engl 41:4337-9. 2002
  53. ncbi A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase
    Susanna Sauret-Güeto
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona Av Diagonal 645, 08028 Barcelona, Spain
    FEBS Lett 580:736-40. 2006
    ....
  54. ncbi Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Jean François Hoeffler
    Université Louis Pasteur CNRS, Institut Le Bel, Strasbourg, France
    Eur J Biochem 269:4446-57. 2002
    ..The equilibrium was, however, largely displaced in favour of the formation of MEP. The reduction step required the presence of a divalent cation such as Mg(2+) or Mn(2+)...
  55. pmc Lethal mutations in the isoprenoid pathway of Salmonella enterica
    Rita M Cornish
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    J Bacteriol 188:1444-50. 2006
    ..The results complement the biochemical and bioinformatic approaches to the elucidation of the genes involved in the MEP pathway and also identify key residues for activity in the enzymes of the pathway...
  56. ncbi Synthesis of (3R,4S)-3,4,5-trihydroxy-4-methylpentylphosphonic acid as a potential inhibitor of the nonmevalonate pathway
    Toshiaki Yoshioka
    Graduate School of Life Sciences, Tohoku University, Sendai, Miygai, Japan
    Biosci Biotechnol Biochem 68:1259-64. 2004
    ..We describe here the synthesis of (3R,4S)-3,4,5-trihydroxy-4-methylpentylphosphonic acid as a candidate for inhibiting MEP cytidylyltransferase, which is the third step on the nonmevalonate pathway...
  57. pmc Characterization of the depletion of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase in Escherichia coli and Bacillus subtilis
    Tracey L Campbell
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    J Bacteriol 184:5609-18. 2002
    ..Synthetic lethal behavior in MEC synthase-depleted cells was prevalent for cell wall-active antibiotics...
  58. pmc Mycoplasma penetrans is capable of activating V gamma 9/V delta 2 T cells while other human pathogenic mycoplasmas fail to do so
    Matthias Eberl
    Biochemisches Institut, Infektiologie, Justus Liebig Universitat Giessen, Friedrichstrasse 24, 35392 Giessen, Germany
    Infect Immun 72:4881-3. 2004
    ..Consequently, this pathogen is capable of stimulating human V gamma 9/V delta 2 T cells...
  59. ncbi Isoprenoid biosynthesis via the MEP pathway. Synthesis of (3,4)-3,4-dihydroxy-5-oxohexylphosphonic acid, an isosteric analogue of 1-deoxy-D-xylulose 5-phosphate, the substrate of the 1-deoxy-D-xylulose 5-phosphate reducto-isomerase
    Odile Meyer
    Universite Louis Pasteur, CNRS UMR 7123, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France
    Org Biomol Chem 1:4367-72. 2003
    ..The enzyme was, however, less efficient with the methylene phosphonate analogue than with the natural substrate...
  60. pmc 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin
    Rakesh K Dhiman
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Bacteriol 187:8395-402. 2005
    ..Thus, M. tuberculosis resistance to fosmidomycin is not due to intrinsic properties of Rv2870c, and the enzyme appears to be a valid drug target in this pathogen...
  61. ncbi Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphoric acid analogues as alternate substrates for methylerythritol phosphate synthase
    David T Fox
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    J Org Chem 70:1978-85. 2005
    ..These results are consistent with a retro-aldol/aldol mechanism rather than an alpha-ketol rearrangement for the enzyme-catalyzed conversion of DXP to MEP...
  62. ncbi Pools of 14C-malic acid as a substrate for pyruvate production for the DOXP/MEP pathway of biosynthesis of carotenoids in chloroplasts
    V S Saakov
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg, 194223 Russia
    Dokl Biochem Biophys 400:7-13. 2005
  63. ncbi Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli
    David T Fox
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 44:8360-8. 2005
    ..These observations are consistent with a retro-aldol/aldol mechanism for the rearrangement during conversion of DXP to MEP...
  64. ncbi Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase
    Argyrides Argyrou
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 43:4375-84. 2004
    ..The results are discussed in terms of significant differences in the commitment factors for the various metal ions and pyridine nucleotides...
  65. pmc Evolution of the isoprene biosynthetic pathway in kudzu
    Thomas D Sharkey
    Department of Botany, University of Wisconsin, Madison, Wisconsin 53706, USA
    Plant Physiol 137:700-12. 2005
    ....
  66. ncbi The interplay between classical and alternative isoprenoid biosynthesis controls gammadelta T cell bioactivity of Listeria monocytogenes
    Maire Begley
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    FEBS Lett 561:99-104. 2004
    ..Vgamma9/Vdelta2 T cell bioactivity is increased in DeltalytB mutants where HMB-PP accumulation is expected, and lost in DeltagcpE mutants which fail to produce HMB-PP...
  67. ncbi Early steps of deoxyxylulose phosphate pathway in chromoplasts of higher plants
    Monika Fellermeier
    Biozentrum der Universität Halle, Weinbergweg 22, D 06120 Halle, Germany
    Phytochemistry 64:199-207. 2003
    ....
  68. ncbi The methylerythritol phosphate pathway is functionally active in all intraerythrocytic stages of Plasmodium falciparum
    Maria B Cassera
    Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, 05508 900 Sao Paulo, Brazil
    J Biol Chem 279:51749-59. 2004
    ..falciparum, and de novo biosynthesis of pyridoxal in a protozoan is reported. Its absence in the human host makes both pathways very attractive as potential new targets for antimalarial drug development...
  69. ncbi Cloning and expression of IspDF from Mesorhizobium loti. Characterization of a bifunctional protein that catalyzes non-consecutive steps in the methylerythritol phosphate pathway
    Charles A Testa
    Department of Chemistry, University of Utah, Salt Lake City, 84112, USA
    Biochim Biophys Acta 1764:85-96. 2006
    ..loti ORF mll0395 complements lethal disruptions in both ispD and ispF in Salmonella typhimurium. These results indicate that IspDF is a bifunctional protein, which catalyzes the first and third steps in the conversion of MEP to cMEDP...
  70. pmc Absence of substrate channeling between active sites in the Agrobacterium tumefaciens IspDF and IspE enzymes of the methyl erythritol phosphate pathway
    Christian Lherbet
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 45:3548-53. 2006
    ..The time dependencies indicate that the enzyme-generated intermediates are not transferred from the IspD active site in IspDF to the active site of IspE or from the active site in IspE to the active site of the IspF module of IspDF...
  71. pmc Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway
    Lorenzo Carretero-Paulet
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, c Marti i Franques 1, 08028 Barcelona, Spain
    Plant Physiol 129:1581-91. 2002
    ..Our results are consistent with the participation of the Arabidopsis DXR gene in the control of the 2-C-methyl-D-erythritol 4-phosphate pathway...
  72. ncbi Biosynthetic origins of the isoprene units of gaudichaudianic acid in Piper gaudichaudianum (Piperaceae)
    Adriana A Lopes
    Instituto de Quimica, Universidade Estadual Paulista, C P 355, CEP 14801 970 Araraquara, SP, Brazil
    Phytochemistry 68:2053-8. 2007
    ..The results confirmed that both plastidic and cytoplasmic pathways are able to provide isopentenyl diphosphate units for prenylation of p-hydroxybenzoic acid...
  73. ncbi Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis
    S B Richard
    Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Biol 8:641-8. 2001
    ..Moreover, these complexes represent the first experimental structures for any cytidyltransferase with both substrates and products bound...
  74. ncbi LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli
    B Altincicek
    Jomaa Pharmaka GmbH, Giessen, Germany
    FEBS Lett 499:37-40. 2001
    ..Cells deleted for the essential lytB gene were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of lytB...
  75. ncbi Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli
    N Campos
    Department de Bioquimica i Biologia Molecular, Universitat de Barcelona, Spain
    FEBS Lett 488:170-3. 2001
    ..Our data demonstrate that the gcpE gene is essential for the MEP pathway in Escherichia coli and indicate that this gene is required for the trunk line of the isoprenoid biosynthetic route...
  76. pmc Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate: a novel system for the genetic analysis of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis
    N Campos
    Department de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, c Marti i Franques 1, 08028 Barcelona, Spain
    Biochem J 353:59-67. 2001
    ..We used this system to show that the ygbP, ychB and ygbB genes are essential in E. coli and that the steps catalysed by the products of these genes belong to the trunk line of the MEP pathway...
  77. ncbi Functional involvement of a deoxy-D-xylulose 5-phosphate reductoisomerase gene harboring locus of Synechococcus leopoliensis in isoprenoid biosynthesis
    B Miller
    Fraunhofer Institut für Atmosphärische Umweltforschung, Kreuzeckbahnstr 19, D 82467, Garmisch Partenkirchen, Germany
    FEBS Lett 481:221-6. 2000
    ....
  78. ncbi A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study
    Francis X Cunningham
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Photosynth Res 92:245-59. 2007
    ..Those cDNAs newly described include ones that encode phytoene synthase, beta-carotene hydroxylase, deoxyxylulose-5-phosphate synthase, isopentenyl diphosphate isomerase, and geranylgeranyl diphosphate synthase...
  79. ncbi Construction and characterization of Escherichia coli disruptants defective in the yaeM gene
    T Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biosci Biotechnol Biochem 63:776-8. 1999
    ..This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli...
  80. pmc Characterization of the Mycobacterium tuberculosis 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase: potential for drug development
    Hyungjin Eoh
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Bacteriol 189:8922-7. 2007
    ..tuberculosis IspD had Km values of 58.5 microM for MEP and 53.2 microM for CTP. Calculated kcat and kcat/Km values were 0.72 min(-1) and 12.3 mM(-1) min(-1) for MEP and 1.0 min(-1) and 18.8 mM(-1) min(-1) for CTP, respectively...
  81. ncbi Contribution of hydroxymethylbutenyl diphosphate synthase to carotenoid biosynthesis in bacteria and plants
    Ursula Flores-Pérez
    Departament de Genètica Molecular de Plantes, Consorci CSIC IRTA, Jordi Girona 18, 08034 Barcelona, Spain
    Biochem Biophys Res Commun 371:510-4. 2008
    ..Our data suggest that enhanced flux through the MEP pathway for peak demand periods in bacteria and plastids does not require increased HDS activity...
  82. pmc Analysis of the isoprenoid biosynthesis pathways in Listeria monocytogenes reveals a role for the alternative 2-C-methyl-D-erythritol 4-phosphate pathway in murine infection
    Maire Begley
    Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
    Infect Immun 76:5392-401. 2008
    ..In vivo bioluminescence imaging also confirmed in vivo expression of the gcpE gene (MEP pathway) during murine infection...
  83. ncbi Are iridoids in leaf beetle larvae synthesized de novo or derived from plant precursors? A methodological approach
    Astrid R B Søe
    Max Planck Institute for Chemical Ecology, Department of Bioorganic Chemistry, Jena, Germany
    Isotopes Environ Health Stud 40:175-80. 2004
    ..Owing to this close similarity in the natural 13C abundances in the plant and insect compounds, a determination of iridoid-origin in leaf beetle secretion may only be possible by use of isotopically labelled compounds...
  84. ncbi Isoprenoid biosynthesis authenticates the classification of the green alga Mesostigma viride as an ancient streptophyte
    Carina Grauvogel
    Institut fur Genetik, Technische Universitat Braunschweig, Spielmannstrasse 7, D 38106 Braunschweig, Germany
    Gene 396:125-33. 2007
    ..Our data indicate that the MVA pathway is a characteristic trait of Plantae in general and propose that it was specifically lost in a common ancestor of Chlorophyta...
  85. pmc Structure of 2C-methyl-D-erythritol 2,4- cyclodiphosphate synthase: an essential enzyme for isoprenoid biosynthesis and target for antimicrobial drug development
    Lauris E Kemp
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Proc Natl Acad Sci U S A 99:6591-6. 2002
    ..Our model will therefore serve as a template to facilitate the structure-based design of potential antimicrobial agents targeting two of the most serious human diseases, tuberculosis and malaria...
  86. ncbi Cloning and functional characterization of 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (GbMECT) gene from Ginkgo biloba
    Sang Min Kim
    Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, Sinlim Dong, Gwanak Gu, Seoul 151 921, Republic of Korea Plant Metabolism Research Center, Kyung Hee University, Yongin 449 701, Republic of Korea
    Phytochemistry 67:1435-41. 2006
    ..Full 88 N-terminal residues were necessary to deliver the protein into the chloroplast as shown by protein-targeting analysis with GFP as a reporter protein in Arabidopsis thaliana protoplasts...
  87. ncbi Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli
    M Hintz
    Jomaa Pharmaka GmbH, Frankfurter Strasse 50, 35392 Giessen, Germany
    FEBS Lett 509:317-22. 2001
    ..HMB-PP is 10(4) times more potent in activating human Vgamma9/Vdelta2 T cells than isopentenyl pyrophosphate...
  88. pmc The relationship between the methyl-erythritol phosphate pathway leading to emission of volatile isoprenoids and abscisic acid content in leaves
    Csengele Barta
    Consiglio Nazionale delle Ricerche, Istituto di Biologia Agroambientale e Forestale, 00016 Monterotondo Scalo, Italy
    Plant Physiol 141:1676-83. 2006
    ..However, stomatal conductance was reduced, indicating that stomatal aperture is not regulated by the MEP-dependent foliar ABA pool, under these specific cases...
  89. pmc Accumulation of a potent gammadelta T-cell stimulator after deletion of the lytB gene in Escherichia coli
    Matthias Eberl
    Biochemisches Institut, Justus Liebig Universitat Giessen, Giessen, Germany
    Immunology 106:200-11. 2002
    ....
  90. ncbi Biosynthesis of terpenoids: efficient multistep biotransformation procedures affording isotope-labeled 2C-methyl-D-erythritol 4-phosphate using recombinant 2C-methyl-D-erythritol 4-phosphate synthase
    S Hecht
    , , Lichtenbergstrasse 4, D-85747 Garching, Germany
    J Org Chem 66:7770-5. 2001
    ..The overall yield based on the respective isotope-labeled starting material is approximately 50%...
  91. pmc Biosynthetic pathway of insect juvenile hormone III in cell suspension cultures of the sedge Cyperus iria
    J C Bede
    Department of Zoology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada M5S 3G5
    Plant Physiol 127:584-93. 2001
    ..iria are similar to the insect pathway and that the final enzymatic reaction in JH III biosynthesis is catalyzed by a cytochrome P(450) monooxygenase...
  92. ncbi Targeting the methyl erythritol phosphate (MEP) pathway for novel antimalarial, antibacterial and herbicidal drug discovery: inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) enzyme
    Nidhi Singh
    Department of Medicinal Chemistry and Laboratory for Applied Drug Design and Synthesis, School of Pharmacy, University of Mississippi, University, MS 38677 1848, USA
    Curr Pharm Des 13:1161-77. 2007
    ....
  93. ncbi Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Biochem 131:313-7. 2002
    ..A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity...
  94. pmc The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum
    Lars Gaigalat
    Institut für Genomforschung, Universitat Bielefeld, D 33594 Bielefeld, Germany
    BMC Mol Biol 8:104. 2007
    ....
  95. pmc Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties
    M Wyss
    VFB Department, F Hoffmann La Roche Ltd, 4070 Basel, Switzerland
    Appl Environ Microbiol 65:367-73. 1999
    ..activity with a broad range of phosphate compounds, including phenyl phosphate, p-nitrophenyl phosphate, sugar phosphates, alpha- and beta-glycerophosphates, phosphoenolpyruvate, 3-phosphoglycerate, ADP, and ATP...
  96. pmc Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase
    P M Nassau
    Glaxo Wellcome Medicines Research Centre, Stevenage, Hertfordshire, United Kingdom
    J Bacteriol 178:1047-52. 1996
    ..The assay is based upon high-pressure liquid chromatography separation of sugar phosphates released from both forms of UDP-galactose by phosphodiesterase treatment...
  97. ncbi Glutamate synthesis in barley roots: the role of the plastidic glucose-6-phosphate dehydrogenase
    Sergio Esposito
    Dipartimento di Biologia Vegetale, Universita di Napoli Federico II, Via Foria 223, 80139 Naples, Italy
    Planta 216:639-47. 2003
    ..Glucose and other sugar phosphates were not as effective in supporting Glu synthesis with respect to Glc-6-P; only ribose-5-phosphate, an ..
  98. ncbi Impaired aerobic glycolysis in muscle phosphofructokinase deficiency results in biphasic post-exercise phosphocreatine recovery in 31P magnetic resonance spectroscopy
    T Grehl
    Department of Neurology, BG Kliniken Bergmannsheil, Ruhr University Bochum, Germany
    Neuromuscul Disord 8:480-8. 1998
    ..glance, the early depletion of P(i) below initial resting levels and the rate of phosphate splitting from sugar phosphates seemed to become the limiting factor for the rate of the oxidative phosphorylation and creatine kinase ..
  99. ncbi Involvement of rapid nucleotide synthesis in recovery from phosphate starvation of Catharanthus roseus cells
    Yuling Yin
    Department of Advanced Bioscience, Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo, 112 8610 Japan
    J Exp Bot 58:1025-33. 2007
    ..Immediately after administration, the most heavily labelled organic compounds are nucleotides, followed by sugar phosphates. Two weeks Pi starvation slowed down the speed of incorporation of (33)P into nucleotides...
  100. ncbi Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis
    U Andersson
    Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund Institute of Technology, Lund University, P O Box 124, Lund SE 221 00, Sweden
    J Biol Chem 276:42707-13. 2001
    ..of trehalose 6-phosphate into beta-glucose 1-phosphate and glucose 6-phosphate by measuring intermediate sugar phosphates in cell extracts from trehalose-cultivated lactococci...
  101. pmc Streamlined pentafluorophenylpropyl column liquid chromatography-tandem quadrupole mass spectrometry and global (13)C-labeled internal standards improve performance for quantitative metabolomics in bacteria
    Song Yang
    Department of Chemical Engineering, University of Washington, Seattle, WA 98195 2180, USA
    J Chromatogr A 1217:7401-10. 2010
    ..All 50 tested metabolite standards from 5 classes (amino acids, carboxylic acids, nucleotides, acyl-CoAs and sugar phosphates) displayed good chromatographic separation and sensitivity on the PFPP column...

Research Grants9

  1. Connecting metabolic stress and cell physiology in Escherichia coli
    GREGORY ROSS RICHARDS; Fiscal Year: 2012
    ....
  2. LENS PROTEIN GLYCATION AND CATARACT DEVELOPMENT
    EDATHARA ABRAHAM; Fiscal Year: 1999
    ..aging and diabetic humans, and during in vitro glycation of rat and human crystallins with various sugars and sugar phosphates. Such information is essential to understand why glycation would induce protein conformational changes, ..
  3. EXOGENOUS INDUCTION OF THE E.COLI UHPT GENE
    ROBERT KADNER; Fiscal Year: 2004
    ..The Escherichia coli uhp locus encodes an active transport system, UhpT, for uptake of sugar phosphates and related substrates...
  4. Role of Inter-alpha Inhibitors in Anthrax Intoxication
    Steven Opal; Fiscal Year: 2007
    ..Our research is focused on the development of a new and safe treatment based on the ability of inter-alpha proteins to prevent the fatal consequences of anthrax infection. [unreadable] [unreadable] [unreadable] [unreadable]..
  5. MOLECULAR BIOLOGY OF TRANSALDOLASE
    Andras Perl; Fiscal Year: 2004
    ..The proposed studies with the availability of unique reagents and existing model systems will further our knowledge on the role of TAL in the PPP, apoptosis signaling, and related disease processes. ..
  6. Mitochondrial dysfunction in patients with SLE
    Andras Perl; Fiscal Year: 2004
    ..Thus, checkpoints of mitochondrial hyperpolarization could represent novel targets of pharmacological intervention in patients with SLE. ..
  7. Interaction of Yersinia with dendritic cells
    Igor Brodsky; Fiscal Year: 2007
    ..This knowledge will be important in the development of future strategies against human pathogens. ..
  8. Genetic and Immunological Impact of the HRES-1/Rab4 Locus in SLE
    Andras Perl; Fiscal Year: 2008
    ..Under Specific Aim 3, we will test the hypothesis that the HindIIIG653C allele, alone or in combination with other genetic factors of lupus-associated haplotypes, enhances the expression of HRES-1/Rab4. ..
  9. BIOSYNTHESIS APPROACH TO NOVEL BIOACTIVE AMINOCYCLITOLS
    Taifo Mahmud; Fiscal Year: 2010
    ..The knowledge and methods that arise from these studies will also be widely applicable to expanding the chemical diversity of other families of bioactive natural products. ..