sepharose

Summary

Top Publications

  1. ncbi Regulation of microfilament organization by Kaposi sarcoma-associated herpes virus-cyclin.CDK6 phosphorylation of caldesmon
    Maria Emanuela Cuomo
    Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, SW3 6JB London, United Kingdom
    J Biol Chem 280:35844-58. 2005
  2. ncbi A self-assembling process in articular cartilage tissue engineering
    Jerry C Hu
    Department of Bioengineering, Rice University, Houston, Texas 77005, USA
    Tissue Eng 12:969-79. 2006
  3. pmc Novel application of S-nitrosoglutathione-Sepharose to identify proteins that are potential targets for S-nitrosoglutathione-induced mixed-disulphide formation
    P Klatt
    Department of Estructura y Función de Proteínas, Centro de Investigaciones Biologicas, Instituto Reina Sofía de Investigaciones Nefrológicas, Consejo Superior de Investigaciones Cientificas, Velazquez 144, E 28006 Madrid, Spain
    Biochem J 349:567-78. 2000
  4. ncbi Purification and characterization of three toxins and two agglutinins from Abrus precatorius seed by using lactamyl-Sepharose affinity chromatography
    R Hegde
    Department of Biochemistry, Indian Institute of Science, Bangalore
    Anal Biochem 194:101-9. 1991
  5. ncbi Quantification of mRNA using real-time PCR and Western blot analysis of MAPK events in chondrocyte/agarose constructs
    David A Lee
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    Methods Mol Biol 695:77-97. 2011
  6. ncbi Integrin-mediated mechanotransduction in IL-1 beta stimulated chondrocytes
    T T Chowdhury
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Biomech Model Mechanobiol 5:192-201. 2006
  7. ncbi Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-Sepharose
    Wentao Hu
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Anal Biochem 398:245-53. 2010
  8. ncbi Dynamic compression inhibits the synthesis of nitric oxide and PGE(2) by IL-1beta-stimulated chondrocytes cultured in agarose constructs
    T T Chowdhury
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, United Kingdom
    Biochem Biophys Res Commun 285:1168-74. 2001
  9. ncbi Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory
    M M Diogo
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    J Chromatogr A 944:119-28. 2002
  10. ncbi Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures
    Terri Ann N Kelly
    Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
    J Biomech 39:1489-97. 2006

Detail Information

Publications224 found, 100 shown here

  1. ncbi Regulation of microfilament organization by Kaposi sarcoma-associated herpes virus-cyclin.CDK6 phosphorylation of caldesmon
    Maria Emanuela Cuomo
    Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, SW3 6JB London, United Kingdom
    J Biol Chem 280:35844-58. 2005
    ..These results establish a novel link between KSHV infection and the regulation of the actin cytoskeleton...
  2. ncbi A self-assembling process in articular cartilage tissue engineering
    Jerry C Hu
    Department of Bioengineering, Rice University, Houston, Texas 77005, USA
    Tissue Eng 12:969-79. 2006
    ..IHC and gel electrophoresis showed collagen type II production and absence of collagen type I. More importantly, self-assembled constructs reached well over one third the stiffness of native tissue...
  3. pmc Novel application of S-nitrosoglutathione-Sepharose to identify proteins that are potential targets for S-nitrosoglutathione-induced mixed-disulphide formation
    P Klatt
    Department of Estructura y Función de Proteínas, Centro de Investigaciones Biologicas, Instituto Reina Sofía de Investigaciones Nefrológicas, Consejo Superior de Investigaciones Cientificas, Velazquez 144, E 28006 Madrid, Spain
    Biochem J 349:567-78. 2000
    ..Here, we show that GSNO-Sepharose mimicks site-specific S-glutathionylation of the transcription factors c-Jun and p50 by free GSNO in vitro...
  4. ncbi Purification and characterization of three toxins and two agglutinins from Abrus precatorius seed by using lactamyl-Sepharose affinity chromatography
    R Hegde
    Department of Biochemistry, Indian Institute of Science, Bangalore
    Anal Biochem 194:101-9. 1991
    ..II, and -III, and two agglutinins, APA-I and -II, were purified from the seeds of Abrus precatorius by lactamyl-Sepharose affinity chromatography followed by gel filtration and DEAE-Sephacel column chromatography...
  5. ncbi Quantification of mRNA using real-time PCR and Western blot analysis of MAPK events in chondrocyte/agarose constructs
    David A Lee
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    Methods Mol Biol 695:77-97. 2011
    ..Ultimately, development of methods used to analyse mechano-sensitive pathways will provide important information for the identification of appropriate pharmacological and physiotherapeutic agents for the treatment of osteoarthritis...
  6. ncbi Integrin-mediated mechanotransduction in IL-1 beta stimulated chondrocytes
    T T Chowdhury
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Biomech Model Mechanobiol 5:192-201. 2006
    ..The non-binding control peptide, GRADSP, had no effect. These data clearly demonstrate that the metabolic response of the chondrocytes to dynamic compression in the presence and absence of IL-1 beta, are integrin mediated...
  7. ncbi Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-Sepharose
    Wentao Hu
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Anal Biochem 398:245-53. 2010
    Activated thiol-Sepharose (ATS) facilitates selection of thiol-containing proteins...
  8. ncbi Dynamic compression inhibits the synthesis of nitric oxide and PGE(2) by IL-1beta-stimulated chondrocytes cultured in agarose constructs
    T T Chowdhury
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, United Kingdom
    Biochem Biophys Res Commun 285:1168-74. 2001
    ..The inhibition of (*)NO and PGE(2) by dynamic compression is a finding of major significance that could contribute to the development of novel strategies for the treatment of cartilage-degenerative disorders...
  9. ncbi Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory
    M M Diogo
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    J Chromatogr A 944:119-28. 2002
    ..interaction chromatography of homo-deoxyoligonucleotides polyA, polyT and polyU with sizes up to 30 bases on a Sepharose gel derivatized with the 1,4-butanediol diglycidyl ether...
  10. ncbi Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures
    Terri Ann N Kelly
    Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
    J Biomech 39:1489-97. 2006
    ....
  11. ncbi Removal of endogenous retrovirus-like particles from CHO-cell derived products using Q sepharose fast flow chromatography
    Daniel M Strauss
    Process Research and Development, Late Stage Purification, Genentech Inc, One DNA Way, South San Francisco, CA 94080, USA
    Biotechnol Prog 25:1194-7. 2009
    ..We have determined the ability of a typical Q sepharose process to remove actual CHO RVLP impurities...
  12. ncbi Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of Cephamandole from Cephalosporin C
    M Terreni
    Pharmaceutical Biocatalysis Laboratories, Dipartimento di Chimica Farmaceutica, Universita degli Studi di Pavia, Via Taramelli 12, 27100, Pavia, Italy
    Bioorg Med Chem Lett 11:2429-32. 2001
    ....
  13. ncbi Nitric oxide-activated glutathione Sepharose
    Peter Klatt
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Campus of Cantoblanco, 28049 Madrid, Spain
    Methods Enzymol 359:245-55. 2002
  14. pmc Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression
    Carole Bougault
    UMR 5086, CNRS, Universite de Lyon, IFR 128, IBCP, Institut de Biologie et Chimie des Proteines, 7 passage du Vercors F 69367 Lyon FRANCE
    BMC Biotechnol 8:71. 2008
    ..The mouse model is of particular interest because of the availability of a large choice of bio-molecular tools suitable to study it, as well as genetically modified mice...
  15. ncbi Antibody purification: affinity chromatography - protein A and protein G Sepharose
    Ana Cristina Grodzki
    Receptors and Signal Transduction Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 588:33-41. 2010
    ..In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.
  16. ncbi Proteomic characterization of protein phosphatase complexes of the mammalian nucleus
    Hue T Tran
    Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    Mol Cell Proteomics 3:257-65. 2004
    ..Microcystin-Sepharose chromatography and mass spectrometry were utilized to affinity purify and identify protein phosphatase-..
  17. ncbi Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression
    M M Knight
    IRC in Biomedical Materials, Queen Mary and Westfield College, London, UK
    Med Eng Phys 20:684-8. 1998
    ..72), although during static compression the cell deformation remained constant. This system may be used to examine cellular events under a range of dynamic mechanical stimuli...
  18. pmc Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs
    T T Chowdhury
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Arthritis Res Ther 10:R35. 2008
    ....
  19. ncbi A protocol for separation and isolation of small and/or large DNA fragments with high yield using CL4B Sepharose
    Kamal Datta
    Department of Nuclear Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    Anal Biochem 317:284-7. 2003
  20. pmc Efficient removal of PCR inhibitors using agarose-embedded DNA preparations
    D Moreira
    Laboratoire de Biologie Cellulaire BC4, URA CNRS 2227, Universite Paris Sud, Bâtiment 444, 91405 Orsay Cedex, France
    Nucleic Acids Res 26:3309-10. 1998
    ....
  21. ncbi Single-step method for purification of Shiga toxin-1 B subunit using receptor-mediated affinity chromatography by globotriaosylceramide-conjugated octyl sepharose CL-4B
    H Nakajima
    Department of Pathology, National Children's Medical Research Center, Taishido, Tokyo, Japan
    Protein Expr Purif 22:267-75. 2001
    ..using receptor-mediated affinity chromatography, in which Gb3Cer (globotriaosylceramide) was conjugated to octyl Sepharose CL-4B as a carrier...
  22. ncbi Bioaffinity immobilization of tannase from Aspergillus niger on concanavalin A-Sepharose CL-4B
    Shweta Sharma
    Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, India
    Biotechnol Appl Biochem 35:165-9. 2002
    Tannase from Aspergillus niger van Teighem was immobilized on concanavalin A-Sepharose via bioaffinity interaction. The immobilized enzyme showed a pH optimum similar to that of the free enzyme...
  23. ncbi Purinergic pathway suppresses the release of .NO and stimulates proteoglycan synthesis in chondrocyte/agarose constructs subjected to dynamic compression
    T T Chowdhury
    Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, UK
    J Cell Physiol 209:845-53. 2006
    ..The current study demonstrates for the first time the importance of a purinergic pathway in mediating the metabolic response to dynamic compression and suppressing an inflammatory effect...
  24. ncbi Improved in vitro development of OPU-derived bovine (Bos taurus) embryos by group culture with agarose-embedded helper embryos
    E M Senatore
    ARSIAL Centro Regionale per la Zootecnia, Rome, Italy
    Theriogenology 74:1643-51. 2010
    ..1%, higher than that in the 3 + 0 group (11.8%, P < 0.05). In conclusion, limited numbers of OPU/IVF oocytes had competent development when cultured with helpers (embedded in agarose to provide physical separation)...
  25. ncbi Rapid purification and characterization of nucleoside diphosphate kinase isoforms using ATP-sepharose affinity column chromatography
    S Y Kim
    College of Pharmacy, Ewha Womans University, Seoul, Korea
    Mol Cells 7:630-4. 1997
    ..with pET3C expression plasmids containing nm23-H1 or nm23-H2, were purified in one step to homogeneity using ATP-sepharose affinity column chromatography...
  26. ncbi Improved isolation of undersampled bacteriophages: finding of distant terminase genes
    Philip Serwer
    Department of Biochemistry, The University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    Virology 329:412-24. 2004
    ..Some had genomes with extremely distant relationships to those of other bacteriophages, based on a tree built from the large terminase genes. These methods find novel genomes by rapidly isolating and screening diverse bacteriophages...
  27. ncbi Dynamic compression counteracts IL-1 beta-induced release of nitric oxide and PGE2 by superficial zone chondrocytes cultured in agarose constructs
    T T Chowdhury
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London, UK
    Osteoarthritis Cartilage 11:688-96. 2003
    ..To examine the effect of IL-1 beta-induced *NO and PGE(2)release by stimulated superficial and deep chondrocyte/agarose constructs subjected to mechanical compression...
  28. ncbi Isolation of soybean trypsin inhibitors by affinity chromatography on anhydrotrypsin-Sepharose 4B
    A Pusztai
    Rowett Research Institute, Bucksburn, Aberdeen, Scotland, United Kingdom
    Anal Biochem 172:108-12. 1988
    ..Inactive material was removed by affinity chromatography on trypsin inhibitor-Sepharose 4B and the purified anhydrotrypsin with full binding capacity for trypsin inhibitors was coupled to cyanogen ..
  29. ncbi Pulsatile dynamic stiffness of cartilage-like materials and use of agarose gels to validate mechanical methods and models
    P Scandiucci de Freitas
    Laboratory for Orthopedic Biomechanics, LOB, University of Basel, c o Biozentrum Pharmazentrum, Klingelbergstrasse 50 70, 4056 Basel, Switzerland
    J Biomed Mater Res B Appl Biomater 78:347-57. 2006
    ..Also, agarose is hygroscopic, leading to unintended variations in gel composition. Agarose gels are problematic materials for validation or comparison of cartilage mechanical test methods and models...
  30. ncbi Purification of Shiga-like toxin 1 by pigeon egg white glycoproteins immobilized on Sepharose gels
    Hiroshi Tomoda
    Kitasato Institute for Life Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo 108 8641, Japan
    Anal Biochem 311:50-6. 2002
    ..Pigeon ovalbumin, ovomucoid, or the whole egg white were immobilized on periodate-oxidized Sepharose CL-6B gels by reductive amination...
  31. ncbi Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain
    David A Lee
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary College, University of London, London, UK
    Methods Mol Med 100:307-24. 2004
    ..The chapter thus provides an introduction to the experimental techniques used to examine chondrocyte mechanotransduction and downstream cell function...
  32. ncbi Inclusion of S-sepharose beads in the culture medium significantly improves recovery of secreted rBPI(21) from transfected CHO-K1 cells
    A H Horwitz
    XOMA US LLC, 1545 17th Street, Santa Monica, California, 90404, USA
    Protein Expr Purif 18:77-85. 2000
    ..To address these issues, we developed a novel process whereby sterile S-Sepharose beads were added directly to the cell culture medium...
  33. ncbi Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression
    Tina T Chowdhury
    Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Arch Biochem Biophys 417:105-11. 2003
    ..The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering...
  34. ncbi Purification of elongation factors EF-Tu and EF-G from Escherichia coli by covalent chromatography on thiol-sepharose
    T D Caldas
    Biochimie Génétique, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, Paris Cedex 05, 75251, France
    Protein Expr Purif 14:65-70. 1998
    ..We took advantage of this property to design a purification protocol based on thiol-Sepharose chromatography, a method involving thiol-disulfide interchange between protein thiol groups and the glutathione-..
  35. ncbi The influence of elaborated pericellular matrix on the deformation of isolated articular chondrocytes cultured in agarose
    M M Knight
    IRC in Biomedical Materials, Queen Mary and Westfield College, Mile End Road, London E1 4NS, UK
    Biochim Biophys Acta 1405:67-77. 1998
    ....
  36. ncbi Heparin elution of transcription factors from DNA-Sepharose columns
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 848:131-8. 1999
    A novel method using heparin for eluting transcription factors from DNA-Sepharose columns was characterized...
  37. ncbi Hydrostatic pressure modulates proteoglycan metabolism in chondrocytes seeded in agarose
    Takashi Toyoda
    Keio University, Tokyo, Japan
    Arthritis Rheum 48:2865-72. 2003
    ..To investigate the effect of isolated hydrostatic pressure on proteoglycan metabolism in chondrocytes...
  38. ncbi Sepharose-unbinding ricin E as a source for ricin A chain immunotoxin
    B H Woo
    Drug Targeting Laboratory, College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, 440-746, Suwon City, South Korea
    J Immunol Methods 249:91-8. 2001
    To evaluate the Sepharose-unbinding ricin E as a preference source material for ricin A chain (RTA) in immunotoxin studies, RTA of ricin E (RTA(E)) was characterized and compared with RTA of the Sepharose-binding ricin D (RTA(D))...
  39. ncbi Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures
    Carole Bougault
    UMR5086, CNRS, IFR128, IBCP Institut de Biologie et Chimie des Protéines, Universite de Lyon, Lyon, France
    Nat Protoc 4:928-38. 2009
    ..The protocols can be carried out with a limited number of mouse embryos or newborns and this point is particularly important regarding genetically modified mice...
  40. ncbi Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs
    S P Raveenthiran
    School of Engineering and Materials Science, Queen Mary, University of London, London, E1 4NS, UK
    Biomech Model Mechanobiol 8:273-83. 2009
    ..The combination of mechanical and pharmacological interventions makes this study a powerful tool to examine further the interactions of biomechanics and cell signalling in osteoarthritis...
  41. ncbi Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffolds
    John D Kisiday
    Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    J Biomech 37:595-604. 2004
    ..Taken together, these results demonstrate the potential of dynamic compression for stimulating PG synthesis and accumulation for applications to in vitro culture of tissue engineered constructs prior to implantation...
  42. ncbi Templated agarose scaffolds support linear axonal regeneration
    Shula Stokols
    Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA
    Tissue Eng 12:2777-87. 2006
    ..The templating process can be useful in fabricating nerve guidance scaffolds for both central and peripheral nerve injuries, or any materials application requiring a precise array of linearly oriented channels...
  43. ncbi Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose
    Greg B G Moorhead
    Department of Biological Sciences, University of Calgary, Alberta, Canada
    Methods Mol Biol 365:39-45. 2007
    ..Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
  44. ncbi Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs
    Tina T Chowdhury
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Biorheology 43:413-29. 2006
    ..These data indicate interactions exist between the NOS and COX pathways, a finding which will provide new insights in the development of pharmacological or biophysical treatments for cartilage disorders such as OA...
  45. ncbi Comparative studies on discrete and concatemeric DNA-sepharose columns for purification of transcription factors
    H Gadgil
    Department of Biochemistry, University of Tennessee, Memphis 38163, USA
    J Chromatogr A 917:43-53. 2001
    ..Our study shows concatemeric columns do not have any distinct advantage for the three different transcription factors we studied including Sp1, the original justification for the concatemeric approach...
  46. ncbi Protein A Sepharose immunoadsorption can restore the efficacy of platelet concentrates in patients with Glanzmann's thrombasthenia and anti-glycoprotein IIb-IIIa antibodies
    Isabelle Martin
    Laboratoire d Hematologie, Centre de Traitement de l Hémophilie, Assistance Publique Hôpitaux de Paris and Faculté de Médecine Paris XI, Le Kremlin Bicetre, Paris, France
    Br J Haematol 119:991-7. 2002
    ..We used immunoadsorption on protein A Sepharose (IA-PA), which has been shown to be efficient in decreasing the titre of antibodies in several immune diseases, ..
  47. ncbi Enzymatically inactive trans-sialidase from Trypanosoma cruzi binds sialyl and beta-galactopyranosyl residues in a sequential ordered mechanism
    Adriane R Todeschini
    Instituto de Biofisica Carlos Chagas Filho, Centro de Ciências da Saúde Bloco G, Universidade Federal do Rio de Janeiro, 21 944970, Cidade Universitaria, Ilha do Fundao, Rio de Janeiro, Brasil
    J Biol Chem 279:5323-8. 2004
    ..cruzi/host cell interaction and could shed more light into the catalytic mechanism of the sialic acid transfer reaction of enzymatically active trans-sialidase...
  48. ncbi Stabilization of pancreatic ribonuclease A by immobilization on Sepharose-linked antibodies that recognize the labile region of the enzyme
    H Younus
    Interdisciplinary Biotechnology Unit, Faculty of Life Sciences, Aligarh Muslim University, India
    Biochim Biophys Acta 1548:114-20. 2001
    ..RNase immobilized on Sepharose support precoupled either with the antipeptide immunoglobulin (IgG) or anti-RNase IgG proved to be more ..
  49. ncbi Boronate-containing copolymers: polyelectrolyte properties and sugar-specific interaction with agarose gel
    Marina V Kuzimenkova
    Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P O Box 124, SE 22100, Lund, Sweden
    Macromol Biosci 6:170-8. 2006
    ..DMAA), and N-isopropylacrylamide (NIPAM) were found to adsorb on cross-linked agarose gel (Sepharose CL-6B) in the pH range from 7.5-9.2, due to specific boronate-sugar interactions...
  50. ncbi Activation of the p70 S6 kinase and phosphorylation of the 4E-BP1 repressor of mRNA translation by type I interferons
    Fatima Lekmine
    Robert H Lurie Comprehensive Cancer Center and Division of Hematology Oncology, Northwestern University Medical School and Lakeside Veterans Administration Medical Center, Chicago, Illinois 60611, USA
    J Biol Chem 278:27772-80. 2003
    ..Altogether, our data establish that the Type I IFN receptor-activated PI 3'-kinase pathway mediates activation of the p70 S6 kinase and inactivation of 4E-BP1, to regulate mRNA translation and induction of Type I IFN responses...
  51. ncbi Monosize poly(glycidyl methacrylate) beads for dye-affinity purification of lysozyme
    Evrim Banu Altıntas
    Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey
    Int J Biol Macromol 38:99-106. 2006
    ..The purity of the eluted lysozyme was analyzed by SDS-PAGE and found to be 88% with recovery about 79%. The specific activity of the eluted lysozyme was high as 43,600 U/mg...
  52. pmc Characterization of two heparan sulphate-binding sites in the mycobacterial adhesin Hlp
    Michelle I Portugal
    Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Av, Brasil 4365, Manguinhos, Rio de Janeiro, RJ 21045 900, Brazil
    BMC Microbiol 8:75. 2008
    ..In the present study, nuclear magnetic resonance (NMR) was used to map the binding site(s) of Hlp to heparan sulfate and identify the nature of the amino acid residues directly involved in this interaction...
  53. ncbi Identification and characterization of beta-D-galactosyl-transferase in chick corneas
    Susumu Sashima
    Radiochemistry Section, Faculty of Pharmacy, Meijo University, Nagoya, Japan
    Jpn J Ophthalmol 46:607-15. 2002
    ..To purify beta-galactosyltransferase, which is involved in the biosynthesis of keratan sulfate, from 2-day-old chick corneas...
  54. pmc Protein-protein interaction assays: eliminating false positive interactions
    Tuan N Nguyen
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    Nat Methods 3:135-9. 2006
  55. ncbi Biosensor-based micro-affinity purification for the proteomic analysis of protein complexes
    B Catimel
    The Ludwig Institute for Cancer Research, Melbourne Tumor Biology Branch, PO Box 2008, Royal Melbourne Hospital, Victoria 3050, Melbourne, Australia
    J Proteome Res 4:1646-56. 2005
    ..The results obtained using the SPU were compared with similar experiments performed using Sepharose beads.
  56. ncbi Screening for beta-poly(L-malate) binding proteins by affinity chromatography
    Thomas Göttler
    Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, D 93040 Regensburg, Germany
    Biochem Biophys Res Commun 341:1119-27. 2006
    ..We have developed an affinity chromatography employing 1,6-diamino-n-hexane-Sepharose-coupled poly(malic acid) to identify such proteins in cellular extracts of Physarum polycephalum...
  57. ncbi 4-N-trimethylaminobutyraldehyde dehydrogenase: purification and characterization of an enzyme from Pseudomonas sp. 13CM
    Maizom Hassan
    United Graduate School of Agricultural Sciences, Tottori University, Koyama, Tottori, Japan
    Biosci Biotechnol Biochem 72:155-62. 2008
    ..The K(m) values for 4-N-trimethylaminobutyraldehyde, 4-dimethylaminobutyraldehyde, and NAD(+) were 7.4, 51, and 125 microM respectively. The enzyme was inhibited by SH reagents, and by heavy metal ions...
  58. ncbi Novel hydrophobic interaction chromatography matrix for specific isolation and simple elution of immunoglobulins (A, G, and M) from porcine serum
    Gabriela Ramos-Clamont
    Centro de Investigación en Alimentación y Desarrollo A C Coordinación de Ciencia de los Alimentos, Apartado Postal 1735, Hermosillo, Sonora 83000, Mexico
    J Chromatogr A 1122:28-34. 2006
    A new, highly acetylated agarose matrix (HA-Sepharose) was synthesized and used as a hydrophobic interaction chromatography (HIC) medium to specifically isolate immunoglobulins (Igs) from porcine serum...
  59. ncbi FCP1 phosphorylation by casein kinase 2 enhances binding to TFIIF and RNA polymerase II carboxyl-terminal domain phosphatase activity
    Benoit Palancade
    UMR 8541 CNRS, Genetique Moleculaire, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    J Biol Chem 277:36061-7. 2002
    ..These findings unravel a new mechanism regulating CTD phosphorylation and hence class II gene transcription...
  60. ncbi Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography
    Tsutomu Arakawa
    Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks, CA 91360, USA
    J Biochem Biophys Methods 70:493-8. 2007
    ..Blue-Sepharose is often used to remove bovine serum albumin (BSA) from samples, but when we applied BSA to Blue-Sepharose in 20 ..
  61. ncbi Chemical and physicochemical characterization of the sialic acid-specific lectin from Cepaea hortensis
    Dieter Gerlach
    Friedrich Schiller University of Jena, Institute of Medical Microbiology, Semmelweisstr 4, Germany
    FEMS Microbiol Lett 214:61-8. 2002
    ..was isolated from the albumin glands of the garden snail Cepaea hortensis by affinity chromatography on fetuin-Sepharose following gel filtration on Superdex 200...
  62. ncbi Galactosyl-mimodye ligands for Pseudomonas fluorescens beta-galactose dehydrogenase
    C F Mazitsos
    Laboratory of Enzyme Technology, Department of Agricultural Biotechnology, Agricultural University of Athens, Greece
    Eur J Biochem 269:5391-405. 2002
    ..Therefore, these new mimodyes suggested by computational analysis, are candidates for application in affinity labeling and structural studies as well as for purification of galactose dehydrogenase...
  63. ncbi A heat shock protein70 fusion protein with alpha1-antitrypsin in plasma of type 1 diabetic subjects
    Paola Finotti
    Department of Pharmacology and Anaesthesiology, University of Padua, Largo E Meneghetti 2, 35131 Padua, Italy
    Biochem Biophys Res Commun 315:297-305. 2004
    ..The additional finding of circulating antibodies against the HSP70-alpha1AT protein supported its immunogenic properties with implications for diabetes and its complications...
  64. ncbi Identification of serine proteases from Leishmania braziliensis
    Herbert L M Guedes
    Laboratório de Bioquímica de Proteínas e Peptídeos, Departamento de Bioquimica e Biologia Molecular, Fundacao Oswaldo Cruz, Rio de Janeiro, RJ, Brazil
    Z Naturforsch C 62:373-81. 2007
    ..amazonensis serine proteases, demonstrating a conservation of this enzymatic class in the Leishmania genus. This is the first study to report the purification of a serine protease from Leishmania braziliensis...
  65. ncbi Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X
    Akira Seko
    Department of Biochemistry, Sasaki Institute, Kanda Surugadai 2 2, Tokyo 101 0062, Japan
    J Biol Chem 278:9150-8. 2003
    ..These results suggested that beta4GalT-IV is a 6SGN-specific beta4GalT and may be involved in the biosynthesis of various glycoproteins carrying a 6-O-sulfated N-acetyllactosamine moiety...
  66. ncbi Nucleotide-mimetic synthetic ligands for DNA-recognizing enzymes One-step purification of Pfu DNA polymerase
    S Melissis
    Laboratory of Enzyme Technology, Department of Agricultural Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR 11855 Athens, Greece
    J Chromatogr A 1122:63-75. 2006
    ..Quality control tests showed that Pfu Pol purified on the B-motif-complementing ligand is free of nucleic acids and contaminating nuclease activities, therefore, suitable for experimental use...
  67. ncbi Purification and characterization of recombinant, human acid ceramidase. Catalytic reactions and interactions with acid sphingomyelinase
    Xingxuan He
    Department of Human Genetics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 278:32978-86. 2003
    ..These studies provide new insights into acid ceramidase and the related lipid hydrolase, acid sphingomyelinase...
  68. ncbi Purification, characterization, and sequence analysis of two alpha-amylase isoforms from azuki bean, Vigna angularis, showing different affinity towards beta-cyclodextrin sepharose
    San San Mar
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Biosci Biotechnol Biochem 67:1080-93. 2003
    ..VAAmy1 apparently had lower affinity towards a beta-cyclodextrin Sepharose column than VAAmy2. Molecular weights of VAAmy1 and VAAmy2 were estimated to be 47,000 and 44,000, respectively...
  69. ncbi ADAM 12 cleaves extracellular matrix proteins and correlates with cancer status and stage
    Roopali Roy
    Program in Vascular Biology and Department of Surgery, Children s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 279:51323-30. 2004
    ..We purified ADAM 12 from the urine of breast cancer patients via Q-Sepharose anion exchange and gelatin-Sepharose affinity chromatography followed by protein identification by matrix-..
  70. ncbi Characterization of growth factor-binding structures in heparin/heparan sulfate using an octasaccharide library
    Satoko Ashikari-Hada
    Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 279:12346-54. 2004
    ..Thus, the library may provide new reagents to specifically regulate bindings of the growth factors to HS...
  71. ncbi Purification of sequence-specific DNA-binding proteins by affinity chromatography
    L A Kerrigan
    University of California San Diego, La Jolla, California, USA
    Curr Protoc Mol Biol . 2001
    ..An provides a method to couple DNA to commercially available CNBr-activated Sepharose, and a support protocol describes how to purify crude synthetic oligonucleotides by gel electrophoresis prior to ..
  72. ncbi Gentamicin binds to the lectin site of calreticulin and inhibits its chaperone activity
    Tomohisa Horibe
    Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, 1 1 1 Noji higashi, Kusatsu, Shiga 525 8577, Japan
    Biochem Biophys Res Commun 323:281-7. 2004
    ..Experiments with domain mutants suggest that the lectin site of CRT is the main target for gentamicin binding and that binding of gentamicin to this site inhibits the chaperone activity of CRT...
  73. ncbi A model for the salt effect on adsorption equilibrium of basic protein to dye-ligand affinity adsorbent
    Songping Zhang
    Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    Biotechnol Prog 20:207-14. 2004
    ..describing the salt effect on adsorption equilibrium of a basic protein, lysozyme, to Cibacron Blue 3GA-modified Sepharose CL-6B (CB-Sepharose) has been developed...
  74. ncbi MeCP2 behaves as an elongated monomer that does not stably associate with the Sin3a chromatin remodeling complex
    Robert J Klose
    Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, United Kingdom
    J Biol Chem 279:46490-6. 2004
    ..Our findings indicate the MeCP2 is not an obligate component of the Sin3a corepressor complex and may therefore engage a more diverse range of cofactors for repressive function...
  75. ncbi Chemically selective displacers for high-resolution protein separations in ion-exchange systems: effect of displacer-protein interactions
    Jia Liu
    Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Ricketts Building, 110 8th Street, Troy, New York 12180, USA
    Anal Chem 80:3357-64. 2008
    ..The ability to exploit protein displacer binding in concert with appropriate displacer resin affinities opens up new possibilities for creating selective displacement systems...
  76. ncbi A binding site for highly sulfated heparan sulfate is identified in the N terminus of the circumsporozoite protein: significance for malarial sporozoite attachment to hepatocytes
    John B Ancsin
    Department of Pathology and Molecular Medicine, Queen s University and the Syl and Molly Apps Research Center, Kingston General Hospital, Kingston, Ontario Canada, K7L 3N6
    J Biol Chem 279:21824-32. 2004
    ..high affinity heparin/HS binding activity and the ability to block the binding of recombinant CSP to heparin-Sepharose 4B. Analysis by isothermal titration calorimetry revealed that region I-plus has a binding constant of K(d) = 5...
  77. ncbi A protein-protein binding assay using coated microtitre plates: increased throughput, reproducibility and speed compared to bead-based assays
    Tim J Craig
    Physiological Laboratory, University of Liverpool, Crown Street, PO Box 147, Liverpool L69 3BX, UK
    J Biochem Biophys Methods 60:49-60. 2004
    ..we have modified this form of binding assay, using glutathione-coated 96-well plates rather than glutathione-Sepharose beads to bind the primary bait protein...
  78. ncbi Rational affinity purification of native Streptomyces family 10 xylanase
    Shigeyasu Ito
    Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990 8560, Japan
    J Biotechnol 110:137-42. 2004
    ..Lactosyl-Sepharose was prepared and tested as an affinity matrix...
  79. ncbi Cloning, characterization, and structural analysis of a C-type lectin from Bothrops insularis (BiL) venom
    Viviane Guimarães-Gomes
    Rede Proteômica do Rio de Janeiro and Laboratório de Hemostase e Venenos LabHemoVen, Departamento de Bioquímica Médica ICB, Universidade Federal do Rio de Janeiro, CEP 21941 590, Rio de Janeiro RJ, Brazil
    Arch Biochem Biophys 432:1-11. 2004
    ..BiL shows similarity to other C-type lectin family members. Modeling studies provide insights into BiL dimeric structure and its structural determinants for carbohydrate and calcium binding...
  80. ncbi The small heat shock protein IbpA of Escherichia coli cooperates with IbpB in stabilization of thermally aggregated proteins in a disaggregation competent state
    Marlena Matuszewska
    Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk, 80 822 Gdansk, Kladki 24, Poland
    J Biol Chem 280:12292-8. 2005
    ..IbpA and IbpB form mixed complexes, and IbpA stimulates association of IbpB with substrate...
  81. ncbi Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins
    Eiji Kinoshita
    Department of Functional Molecular Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734 8551, Japan
    J Sep Sci 28:155-62. 2005
    ..The affinity gel (Phos-tag agarose) was prepared by the quantitative reaction of N-hydroxysuccinimide-activated Sepharose and a Phos-tag derivative having a 2-aminoethylcarbamoyl group in dry CH3CN...
  82. ncbi Evaluation of a novel agarose-based synthetic ligand adsorbent for the recovery of antibodies from ovine serum
    Sunil Chhatre
    The Advanced Centre for Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom
    J Chromatogr B Analyt Technol Biomed Life Sci 860:209-17. 2007
    ..that the binding characteristics of the prototype adsorbent are similar to those obtained with Protein G Sepharose. This study indicates that the high-capacity prototype matrix is a feasible and potentially cost-effective ..
  83. ncbi Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin
    James N Arnold
    Medical Research Council Immunochemistry Unit, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 280:29080-7. 2005
    ..Antigen-bound IgM pentamers therefore do not activate complement via the lectin pathway, but MBL might have a role in the clearance of aggregated IgM...
  84. ncbi Trypanosoma cruzi: partial characterization of minor cruzipain isoforms non-adsorbed to Concanavalin A-Sepharose
    Vilma G Duschak
    Instituto de Investigaciones Biotecnologicas, Universidad Nacional de General San Martin, Av Gral Paz y Albarellos, Parque Tecnológico Miguelete, INTI Edificio 24, 1650 San Martin, Prov Buenos Aires, Argentina
    Exp Parasitol 104:122-30. 2003
    ..the partial characterization of a subset of atypical cruzipain molecules which do not bind to Concanavalin A-Sepharose column...
  85. ncbi Selective binding of the scavenger receptor C-type lectin to Lewisx trisaccharide and related glycan ligands
    Peter J Coombs
    Glycobiology Institute, Department of Biochemistry, University of Oxford, UK
    J Biol Chem 280:22993-9. 2005
    ..These studies suggest that SRCL might be involved in selective clearance of specific desialylated glycoproteins from circulation and/or interaction of cells bearing Lewis(x)-type structures with the vascular endothelium...
  86. ncbi Isolation and characterization of a microsomal acid retinyl ester hydrolase
    Thomas Linke
    Phytonutrients Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Biol Chem 280:23287-94. 2005
    ..Purification to homogeneity was achieved by sequential chromatography using SP-Sepharose cation exchange, phenyl-Sepharose hydrophobic interaction, concanavalin A-Sepharose affinity and Superose 12 gel ..
  87. ncbi An inter-subunit disulfide bond affects affinity of human lung extracellular superoxide dismutase to heparin
    Tomomi Ookawara
    Department of Biochemistry, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo 663 8501, Japan
    Free Radic Res 37:823-7. 2003
    ..Moreover, the results obtained here also suggest that the redox state of the tissues might regulate the function of the EC-SOD...
  88. ncbi [Purification and identification of human recombinant IFN-beta expressed in yeast Pichia pastoris]
    Qi wen Yu
    Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:1035-9. 2003
    ..of purifying and identifying recombinant human IFN-beta (rhIFN-beta) expressed in yeast Pichia pastoris, Blue Sepharose 6 fast flow (Blue S6FF) and immunological affinity chromatography (IAC) were compared in this report...
  89. ncbi Purification of recombinant human carbonic anhydrase-II by metal affinity chromatography without incorporating histidine tags
    Abir L Banerjee
    Department of Chemistry and Molecular Biology, North Dakota State University, Fargo, ND 58105, USA
    Protein Expr Purif 37:450-4. 2004
    ..We discovered that hCA-II preferentially binds to the Sepharose-iminodiacetate (IDA)-Zn(2+) column, and such binding does not require incorporation of either N- or C-terminal ..
  90. ncbi The preferred pathway of glycosaminoglycan-accelerated inactivation of thrombin by heparin cofactor II
    Ingrid M Verhamme
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2561, USA
    J Biol Chem 279:9785-95. 2004
    ..did not attenuate the DS-accelerated thrombin inactivation markedly, but DS displaced thrombin from heparin-Sepharose, suggesting that DS and heparin share a restricted binding site in or nearby exosite II, in addition to binding ..
  91. ncbi Sample displacement chromatography of Atlantic Salmon (Salmo salar) thrombin
    Even Manseth
    Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, Chr M Falsensgate 1, P O Box 5040, N 1432 As, Norway
    J Biochem Biophys Methods 60:39-47. 2004
    ..chromatography system with non-homogenous sample application in the order of increasing affinity to Heparin Sepharose. The results suggest that this modified method could be useful in protein purification...
  92. ncbi Refolding and purification of recombinant human PDE7A expressed in Escherichia coli as inclusion bodies
    Wito Richter
    Institute of Biochemistry, Medical Faculty, Institute of Biochemistry, University of Leipzig, Liebigstrasse 16, Leipzig, Germany
    Protein Expr Purif 25:138-48. 2002
    ..5. The PDE7A1(147-482-His) construct could be purified after dialysis and concentration steps by either Zn2+-IDA-Sepharose chromatography or ResourceQ ion-exchange chromatography to homogeneity...
  93. ncbi Biochemical properties and cDNa cloning of two new lectins from the plasma of Tachypleus tridentatus: Tachypleus plasma lectin 1 and 2+
    S C Chen
    Institute of Biological Chemistry, Academia Sinica, Nankang Borough, Taipei 115, Taiwan, Republic of China
    J Biol Chem 276:9631-9. 2001
    A Sepharose CL-4B-binding protein, Tachypleus plasma lectin 1 (TPL-1), and a lipopolysaccharide (LPS)-binding protein, Tachypleus plasma lectin-2 (TPL-2), have been isolated from the plasma of Tachypleus tridentatus and biochemically ..
  94. ncbi Lactate dehydrogenase from the northern krill Meganyctiphanes norvegica: comparison with LDH from the Antarctic krill Euphausia superba
    E Mulkiewicz
    , , Poland
    Comp Biochem Physiol B Biochem Mol Biol 128:233-45. 2001
    ..Antarctic krill isoenzyme exhibits high substrate affinities, high NAD inhibition, high inhibition at 10 mM pyruvate, lack of lactate inhibition, and high heat stability and resembles northern krill LDH-A*(4) isoenzyme...
  95. ncbi Phenylboronic acid-salicylhydroxamic acid bioconjugates. 2. Polyvalent immobilization of protein ligands for affinity chromatography
    J P Wiley
    Prolinx, Inc, 22322 20th Avenue SE, Bothell, Washington 98021, USA
    Bioconjug Chem 12:240-50. 2001
    ..were compared with respect to the efficiency with which they were immobilized on salicylhydroxamic acid-modified Sepharose (SHA-X-Sepharose) by boronic acid complex formation. When immobilized on moderate capacity SHA-X-Sepharose (5...
  96. ncbi Novel lectin-like proteins on the surface of human monocytic leukemia cell line THP-1 cells that recognize oxidized cells
    S Eda
    School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Arch Biochem Biophys 385:186-93. 2001
    ..macrophages were solubilized, and the membrane proteins obtained by affinity chromatographies using lactoferrin-Sepharose and band 3-Sepharose were purified by successive DE column chromatography and sodium dodecyl sulfate-..
  97. ncbi Comprehensive study on binding capacity of human immunoglobulin G to Avid AL affinity gel
    J Y Shi
    Unisyn Technologies, Inc, Tustin, CA 92680
    J Chromatogr A 686:61-71. 1994
    ..2 mg/ml gel using binding buffer containing sodium sulfate. Recombinant Protein A gel was used as a control. In this study it also exhibited enhanced binding capacity with a high salt concentration binding buffer...
  98. ncbi Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase
    H H Mu
    Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick 08901, USA
    Arch Biochem Biophys 388:155-64. 2001
    ..included ammonium sulfate fractionation, Sephacryl 300 HR chromatography, affinity starch adsorption, Q-Sepharose, and Mono Q chromatography...
  99. ncbi Protein adsorption on histidyl-aminohexyl-Sepharose 4B. II. Application to the negative one-step affinity purification of human beta2-microglobulin and immunoglobulin G
    O Pitiot
    , , CNRS-UPRESA 6 022, France
    J Chromatogr B Biomed Sci Appl 758:173-82. 2001
    ..from uremic patient's blood ultrafiltrate and plasma, respectively, was investigated on the histidyl-aminohexyl-Sepharose 4B adsorbent...
  100. ncbi Isolation of a novel agglutinin with complex carbohydrate binding specificity from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji
    T B Ng
    Department of Biochemistry, Chinese University of Hong Kong, Shatin, N T, Hong Kong, People s Republic of China
    Biochem Biophys Res Commun 290:563-8. 2002
    ..The protein was adsorbed on CM-Sepharose even in 20 mM ammonium acetate (pH 5...
  101. ncbi Sephadex-based cell-affinity adsorbents: preparation and performance
    Geert Besselink
    Department of Transfusion Technology, CLB, Sanquin Blood Supply Foundation, P O Box 9190, 1066 AC Amsterdam, The Netherlands
    Biotechnol Appl Biochem 35:55-60. 2002
    ..For comparison, cell binding to commercially obtained SpA-Sepharose was determined...

Research Grants62

  1. Mechanisms of tubular atrophy in renal disease
    Jeffrey R Schelling; Fiscal Year: 2012
    ..cells, phospholipids overlays and displacement of fluorescently labeled PI(4,5)P2 bound to NHE1 immobilized on Sepharose beads...
  2. Engineering a packaging nanomotor for delivery of RNA and other molecules
    Venigalla B Rao; Fiscal Year: 2010
    ..The his-tagged mutants will be overexpressed in E.coli and purified by affinity chromatography using 96-well Ni-Sepharose plates...
  3. Kinome Reprogramming in Response to Targeted Kinase Inhibitors
    Gary L Johnson; Fiscal Year: 2013
    ..Our methods utilize Multiplexed Inhibitor Beads (MIBs), consisting of Sepharose beads with covalently immobilized, linker adapted, kinase inhibitors...
  4. NEMATODE-INDUCED PULMONARY GRANULOMA FORMATION
    STEPHEN KAYES; Fiscal Year: 1991
    ..as regards their role in the induction and elicitation of artificial pulmonary granulomas that form around sepharose beads covalently coated with Toxocara exoantigens (TEX)...
  5. Tropomyosin in the antiangiogenic activity of HKa
    Keith McCrae; Fiscal Year: 2007
    ..Finally, affinity purification of chymotrypsin-digested tropomyosin on HKa-sepharose, as w ell as panning of a cyclic random peptide Library on HKa, led to tentative identification of HKa binding ..
  6. Effects of Alzheimer's disease on hippocampal alpha7-nAChR protein interactors
    Edward Hawrot; Fiscal Year: 2012
    ..its interacting proteins will be isolated from the homogenate by ligand affinity pulldown (?-bungarotoxin[Bgtx]-Sepharose beads)...
  7. ISOLATION OF ELONGATION ENZYMES & SITE OF SYNTHESIS
    DOMINICK CINTI; Fiscal Year: 1991
    ..Antibodies to the component enzymes will be prepared from rabbits, purified by employing Protein A sepharose and utilized to study turnover of the enzyme components in the microsomal membrane and to study the site of ..
  8. LIGAND RECEPTOR INTERACTIONS IN BACTERIAL ADHERENCE
    SOMAN ABRAHAM; Fiscal Year: 1993
    ..to and elution from mannan containing yeast cells or by ultrafiltration and gel filtration on columns of Sepharose 4B...
  9. CARBOHYDRATE-CONTAINING MACROMOLECULES IN AGING BRAIN
    ERIC BRUNNGRABER; Fiscal Year: 1980
    ..Glycopeptides that act as receptors for con A are separated by means of affinity chromatography (Con A-sepharose); this fraction will be subdivided to yield neutral and basic mannose-rich glycopeptides as well as an acidic ..
  10. CARHOHYDRATE MOIETIES OF GP120
    Khushi Matta; Fiscal Year: 1993
    ..The availability of antigen moiety linked to a solid support (Sepharose) will facilitate purification of the antibodies raised against the corresponding antigens...
  11. LYMPHATIC LESION PATHOGENESIS IN BRUGIA-INFECTED JIRDS
    THOMAS KLEI; Fiscal Year: 1991
    ..of worm extracts or secretions will be tested in vivo for their ability to induce granulomas when coupled to sepharose beads and embolized into the lungs of jirds with acute and chronic infections...
  12. MECHANISMS FOR INTESTINAL BILE ACID TRANSPORT
    Frederick Wilson; Fiscal Year: 1990
    ..subcellular fractions are solubilized and subjected to protein purification using a glycocholate derivative - Sepharose- 4B affinity column...
  13. IMMUNOREGULATORY EFFECTS OF THE INTERFERONS
    Howard Ozer; Fiscal Year: 1992
    ..receptor will be accomplished using affinity chromatography on interferonsepharose, on anti-receptor antibody sepharose, on wheat germ sepharose, and with HPLC...
  14. Altered endothelial heparan sulfate causes diaphragmatic hernias resembling CDH
    Jeffrey Esko; Fiscal Year: 2007
    ..Recombinant Slit3 protein has been produced in cultured cells and shown to bind heparin-Sepharose. The binding of recombinant Slit3 protein to wildtype and heparan sulfate-deficient endothelial cells will be ..
  15. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ..The investigators will also purify this PPase using microcystin -sepharose affinity columns to determine changes in the (A, B and C) subunit composition of PP2A under various experimental ..
  16. ROLE OF PHOSPHOLIPIDS IN MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1992
    ..based on the results of endothelial cell chemotaxis assays of extracted tissues and culture media after heparin- Sepharose chromatography, Western blots of isolated growth factors and immunolocalization with specific antibodies...
  17. AVIDIN-BIOTIN TECHNOLOGY FOR HORMONE RECEPTOR ISOLATION
    Klaus Hofmann; Fiscal Year: 1990
    ..and the ensuing hormone-receptor complex will be isolated from the detergent solubilized membranes on columns of Sepharose 4B immobilized succinoylvidin...
  18. ROLE OF RIBONUCLEOTIDE REDUCTASE IN DNA REPLICATION
    C Greenberg; Fiscal Year: 1990
    ..The defect in nrdB93 can be demonstrated in vivo by the rate of deoxyribonucleotide synthesis or in vitro by Sepharose-dATP columns and by two dimensional electrophoresis...
  19. CARBOHYDRATE BINDING PROTEIN OF B. JAPONICUM
    SIUCHEONG HO; Fiscal Year: 1993
    ..with the behavior of intact Rhizobium in four carbohydrate-specific binding assays: (a) adsorption to Lac-Sepharose beads; (b) heterotypic binding to cultured soybean (SB-1) cells; (c) heterotypic adhesion to soybean roots; and (..
  20. TRANSLATIONAL CONTROL OF PROTEIN SYNTHESIS BY HEMIN
    Martin Gross; Fiscal Year: 1990
    ..under a variety of conditions in the intact lysate and with ribosomes isolated from lysate by chromatography on Sepharose 6B...
  21. STUDIES OF IGE DEPENDENT HISTAMINE RELEASING FACTORS
    Susan MacDonald; Fiscal Year: 1991
    ..responsiveness to HRF is dependent on IgE: it is ablated by heat treating serum, by absorbing serum on anti-IgE Sepharose, and by mixing serum with excess IgE myeloma...
  22. PHOSPHOLIPIDS AND MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1999
    ..based on the results of endothelial cell chemotaxis assays of extracted tissues and culture media after heparin- Sepharose chromatography, Western blots of isolated growth factors and immunolocalization with specific antibodies...
  23. ANDROGEN RECEPTORS IN CANCEROUS PROSTATIC TISSUE
    Donald Tindall; Fiscal Year: 1993
    ..These techniques included both differential binding to DNA-Sepharose and testosterone-affinity chromatography...
  24. GENE TRANSFER TO AIRWAY EPITHELIAL CELLS
    Pi Wan Cheng; Fiscal Year: 2000
    ..We propose to test the hypothesis that the liposome-receptor ligand-DNA complex by Sepharose gel chromatography and then characterize the physicochemical properties of the putative transfection complex by ..
  25. LEUKOCYTE INTERACTIONS WITH EXTRACELLULAR MATRIX
    ARTHUR MERCURIO; Fiscal Year: 1999
    ..data on the 67kD and 36kD LBPs will be obtained by sequencing peptides derived from LBPs purified by laminin-Sepharose chromatography...
  26. REGULATION OF POLYCYCLIC AROMATIC HYDROCARBON TOXICITY
    WILLIAM HOUSER; Fiscal Year: 1992
    ..protein by affinity chromatography utilizing oligonucleotides containing consensus binding sequences coupled to Sepharose and; 6) Prepare AHH deficient rat H4-II-E cell lines analogous to those prepared from mouse Hepa-1 cells...
  27. CHARACTERIZATION OF THE LEUKOCYTE INS (1,4,5)P3 RECEPTOR
    PETER BRADFORD; Fiscal Year: 1991
    ..an affinity support using p-amino benzoiacid derivatized inositol 1,4,5-trisphosphate coupled to an activated CH-sepharose 4B matrix...
  28. ENZYMATIC N-METHYLATION OF CALMODULIN
    FRANK SIEGEL; Fiscal Year: 1993
    ..The two calmodulins will be separated and quantitated by sequential melittin-Sepharose chromatography and ion-exchange FPLC...
  29. NEUROSPORA DNA REPAIR
    Alice Schroeder; Fiscal Year: 1980
    ..focusing, they are very similar in salt, Mg ions and pH optima and show identical chromatographic behavior on Sepharose-Blue Dextran, DEAE, and DNA-cellulose. Further characterization of these activities will be undertaken...
  30. ROLE OF OSTEOPONTIN IN GRANULOMATOUS INFLAMMATION
    Jeffrey Berman; Fiscal Year: 2003
    ..A model will be used in which granulomas are induced by the embolization of sepharose beads containing antigens which induce the formation of granulomas dependent on Th1 and Th2 cytokines...
  31. ANTIMALARIAL ACTION AND RESISTANCE
    Donald Krogstad; Fiscal Year: 1991
    ..be identified by its ability to bind chloroquine using: gel electrophoresis and autoradiography, chloroquine-- sepharose column chromatography, inhibition of chloroquine accumulation by antibodies to a reconstituted vesicle ..
  32. GLYCOSYLATION IN EXPERIMENTAL CARCINOGENESIS
    S Rajalakshmi; Fiscal Year: 1992
    ..using a new affinity chromatography absorbant in which UDP-N-acetylglucosamine has been linked to thiopropyl-sepharose at the 5 position of the uracil via a 5-mercurimercaptide bond...
  33. PLASMINOGEN/ANGIOSTATIN CONVERTING ENZYME
    Gerald Soff; Fiscal Year: 2000
    ..These include Reactive Red 120-Agarose (Dye-Ligand chromatography), Hi-Q-Sepharose (anion exchange chromatography) and soybean trypsin inhibitor-sepharose (affinity chromatography)...
  34. NOVEL GLYCOCONJUGATE-BINDING PROTEINS OF LEUCOCYTES
    HOWARD ALLEN; Fiscal Year: 1990
    ..Galaptin will be isolated from normal human peripheral leucocytes by asialofetuin-Sepharose affinity chromatography...
  35. Proteomics of M-L antigens modulating cation transport
    PETER LAUF; Fiscal Year: 2004
    ..M antisera by affinity chromatography on LK and HK SRBC membrane ghosts covalently immobilized on CNBr-activated Sepharose. 2...
  36. PURIFICATION OF BOVINE PLASMINOGEN PROACTIVATOR
    ROBERT RADCLIFFE; Fiscal Year: 1980
    ..The same series of precipitations followed by chromatography on lysine Sepharose works also for bovine activator, but the yield is lower...
  37. Reagents to Investigate Glycosylated Macromolecules
    DOUG CHAN; Fiscal Year: 2007
    ..g., glass or sepharose beads) with or without a photocleavable linker; and (iii) fluorescent, radiolabeled, or other analytical ..
  38. STRUCTURE AND FUNCTION STUDIES ON HEPATIC LIPASE
    MICHAEL KOMAROMY; Fiscal Year: 1992
    ..The proteins will be tested for their ability to bind to heparin-Sepharose. Further subcloning of the binding region will completely define the site...
  39. CARNITINE PALMITOYLTRANSFERASE I ISOFORM FUNCTION
    Tod Gulick; Fiscal Year: 2005
    ..This will be correlated with isozyme submito loci and topology in parallel strategies: 1. Efficacy of Sepharose-coupled substrate and mal-CoA (which are cytosol-restricted) on isozyme activity; 2...
  40. GENETIC CONTROL OF RIBOSOME ASSEMBLY
    PAUL SYPHERD; Fiscal Year: 1980
    ..5 M NH4Cl wash of ribosomes. 2. The factor binds to DEAE at PH 7.0. 3. The factor binds to sepharose at low ionic strength. 4. Activity is heat labile (10 min at 50C). 5. Factor is not inactivated by NEM or PCMB...
  41. BIOCHEMISTRY OF NORMAL AND LEUKEMIC LYMPHOCYTES
    CREED ABELL; Fiscal Year: 1980
    ..detect specific surface components involved in the activation, membrane preparations will be resolved by lectin-Sepharose chromatography and by two-dimensional gel electrophoresis...
  42. MECHANISTIC STUDIES OF HYDROXYLASES
    JOSEPH VILLAFRANCA; Fiscal Year: 1992
    ..the purification of the enzyme by isolating monoclonal antibodies to PAM for the preparation of antibody-linked Sepharose columns...
  43. INTERACTION OF PROGESTERONE RECEPTOR WITH ATP
    David Toft; Fiscal Year: 1980
    ..The binding of receptor to ATP has been shown using ATP-Sepharose affinity chromatography. This binding is reversible and shows a preference for ATP...
  44. GLUCOSE MEDIATED POLYMERIC INSULIN DELIVERY SYSTEM
    Larry Brown; Fiscal Year: 1990
    ..In this system, glucose oxidase is immobilized to Sepharose beads or to the polymer backbone of the insulin delivery matrix...
  45. Small molecule microarrays for intracellular proteins
    Kit Lam; Fiscal Year: 2005
    ..Selected small molecule ligands will be resynthesized on Sepharose beads and used as affinity matrix to capture the binding proteins or protein-complexes...
  46. MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
    Daniel Goodenough; Fiscal Year: 1993
    ..has been demonstrated to be competent to rebind to tight junctions at the morphologically correct sites, will be sepharose- coupled for affinity chromatography...
  47. CONTROL OF MACROPHAGE ANTIGEN-PRESENTING FUNCTION
    David Beller; Fiscal Year: 1991
    ..the basis for the preferential response of selected T cells to mIL-1 or to IL-1 that has been crosslinked on sepharose. These studies will include evaluation of sIL-1 responsive and non-responsive subclones for number, affinity, ..
  48. 4-Hydroxyestradiol Receptor in Breast Cancer
    Jose Russo; Fiscal Year: 2005
    ..Specifically, we plan to: 1. Synthesize 4-hydroxyestradiol-linked sepharose 6B and use it to isolate the receptor from mouse uterine tissue, and 2...
  49. T CELL COLONY FORMATION IN NORMALS AND AIDS
    ALAN WINKELSTEIN; Fiscal Year: 1991
    ..synthesis assay and (6) comparisons of the mitogenic activities of phorbol myristate acetate (TPA) and Sepharose-bound T3 with PHA. These compounds appear to activate T cells by mechanisms distinct from those expressed by PHA...
  50. CELLULAR MECHANISMS OF LUNG DEVELOPMENT
    Alan Stiles; Fiscal Year: 1991
    ..radioactive amino acid labelling of secreted proteins, affinity chromatography with SmC antibodies coupled to sepharose and HPLC purification of the antibody bound radio-labelled proteins...
  51. CIRCULATING AND CELL BOUND IMMUNE COMPLEXES
    ARGYRIOS THEOFILOPOULOS; Fiscal Year: 1980
    ..2. Attempts will be made to isolate serum immune complexes using Raji cells, conglutinin-Sepharose or staphylococcal protein A-Sepharose as in vitro adsorbents. 3...
  52. IDENTIFICATION OF TSP RECEPTORS ON HUMAN CELLS
    Martha Aiken; Fiscal Year: 1992
    ..Either TSP, an isolated domain of TSP, or TSP peptide retaining platelet binding capacity will be coupled to Sepharose. EDTA and TSP will be used to elute the bound receptor from the column...
  53. A Meningococcal LOS Vaccine
    JOHN GRIFFISS; Fiscal Year: 2009
    ..We purified human LOS IgGs by passage of IVIG through Sepharose to which we coupled L1;L1,1a,8;L2,4;L8v;L2;and L7 LOS...
  54. REGULATION OF EXOCYTOSIS IN ALVEOLAR TYPE II CELLS
    Mark Pian; Fiscal Year: 1992
    ..The effect of preventing internalization of SP-A coupled to Sepharose will also be tested...
  55. CYCLIC AMP DEPENDENT PROTEIN KINASES IN HYPERTENSION
    Diwan Singh; Fiscal Year: 1980
    ..Use of 8-aminohexyl ATP-Sepharose 4B along with the most commonly used purification procedures will be employed to achieve this goal...
  56. Assembly Visualized Routine Flourescence Microscopy
    Philip Serwer; Fiscal Year: 2008
    ..Applications are anticipated in the analysis of the assembly of disease-causing viruses and determining pathways of oncogenesis. ..
  57. Enhanced Capillary DNA Sequencing
    Philip Serwer; Fiscal Year: 2004
    ..Each adaptation will help solve a current genomic problem, for example, either closing of a partially assembled genome sequence or performing high accuracy analysis of mutations. ..
  58. DNA Packaging Motors
    Philip Serwer; Fiscal Year: 2005
    ..Analysis of bacteriophage DNA packaging motors is expected to provide a basis for understanding the role of motors in disease, especially disease caused by a virus that has a DNA packaging motor. ..
  59. Novel Antioxidant Mechanisms of Surfactant Proteins
    Francis McCormack; Fiscal Year: 2006
    ..The results will provide important insights into the roles of the surfactant proteins in preventing oxidative lung disease and may be useful for the design of novel recombinant proteins and peptides of therapeutic value. ..
  60. SURFACTANT PROTEIN A IN PULMONARY HOST DEFENSE
    Francis McCormack; Fiscal Year: 2002
    ..These studies will elucidate the role of SP-A in the innate immune defense of the lung, and may assist in the design of optimal antimicrobial strategies. ..
  61. Lymphangioleiomyomatosis Research Project
    Francis McCormack; Fiscal Year: 2002
    ..Efforts have been made to attract established investigators from related disciplines who can complement the expertise of the LAM investigators. ..
  62. Lymphangioleimyomatosis Research Conference
    Francis McCormack; Fiscal Year: 2004
    ..abstract_text> ..