heparitin sulfate

Summary

Summary: A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.

Top Publications

  1. pmc Human papillomavirus infection requires cell surface heparan sulfate
    T Giroglou
    Institute for Medical Microbiology and Hygiene, University of Mainz, 55101 Mainz, Germany
    J Virol 75:1565-70. 2001
  2. ncbi The structure of glycosaminoglycans and their interactions with proteins
    Neha S Gandhi
    Western Australian Biomedical Research Institute, Curtin University of Technology, Perth, WA 6845, Australia
    Chem Biol Drug Des 72:455-82. 2008
  3. ncbi Sanfilippo syndrome: a mini-review
    M J Valstar
    Department of Pediatrics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Inherit Metab Dis 31:240-52. 2008
  4. ncbi C. elegans Kallmann syndrome protein KAL-1 interacts with syndecan and glypican to regulate neuronal cell migrations
    Martin L Hudson
    Department of Molecular, Cell, and Developmental Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA
    Dev Biol 294:352-65. 2006
  5. ncbi FGFs, heparan sulfate and FGFRs: complex interactions essential for development
    D M Ornitz
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioessays 22:108-12. 2000
  6. pmc Productive dengue virus infection of human endothelial cells is directed by heparan sulfate-containing proteoglycan receptors
    Nadine Dalrymple
    Department of Molecular Genetics and Microbiology, Stony Brook University, Life Sciences Rm 126, Stony Brook, NY 11794 5222, USA
    J Virol 85:9478-85. 2011
  7. ncbi Endothelial cell glycocalyx modulates immobilization of leukocytes at the endothelial surface
    Alina A Constantinescu
    Department of Medical Physics, University of Amsterdam, Amsterdam, The Netherlands
    Arterioscler Thromb Vasc Biol 23:1541-7. 2003
  8. pmc Extracellular sugar modifications provide instructive and cell-specific information for axon-guidance choices
    Hannes E Bülow
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Curr Biol 18:1978-85. 2008
  9. pmc Perlecan maintains the integrity of cartilage and some basement membranes
    M Costell
    Department of Experimental Pathology, Lund University, S 221 85 Lund, Sweden
    J Cell Biol 147:1109-22. 1999
  10. ncbi Differential sulfations and epimerization define heparan sulfate specificity in nervous system development
    Hannes E Bülow
    Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY 10032 USA
    Neuron 41:723-36. 2004

Research Grants

  1. The Glycocalyx and Endothelial Barrier Regulation
    RANDAL DULL; Fiscal Year: 2005
  2. Strategy for Therapy of the Pseudoachondroplasia (PSACH) Phenotype: Understanding
    Jacqueline Hecht; Fiscal Year: 2006
  3. Correction of neural abnormalities in Hurler syndrome
    Pankaj Gupta; Fiscal Year: 2003
  4. Correction of Hurler syndrome by multipotent stem cells
    Pankaj Gupta; Fiscal Year: 2007
  5. PATHOBIOCHEMISTRY OF PROTEOGLYCANS IN DEVELOPMENT
    Scott Saunders; Fiscal Year: 2009
  6. Genetic Studies of Clubfoot (ITEV)
    Jacqueline T Hecht; Fiscal Year: 2010
  7. Microenvironment Modulates HIV Replication in Macrophage
    Philippe Gallay; Fiscal Year: 2007
  8. BIOLOGY OF BAMACAN IN TUMORIGENESIS
    Giancarlo Ghiselli; Fiscal Year: 2006
  9. 2006 Proteoglycans Gordon Research Conferences
    ALAN RAPRAEGER; Fiscal Year: 2006
  10. Heparan sulfate in neural development
    Yu Yamaguchi; Fiscal Year: 2007

Detail Information

Publications280 found, 100 shown here

  1. pmc Human papillomavirus infection requires cell surface heparan sulfate
    T Giroglou
    Institute for Medical Microbiology and Hygiene, University of Mainz, 55101 Mainz, Germany
    J Virol 75:1565-70. 2001
    ..Alpha-6 integrin is not a receptor for HPV-33 pseudoinfection...
  2. ncbi The structure of glycosaminoglycans and their interactions with proteins
    Neha S Gandhi
    Western Australian Biomedical Research Institute, Curtin University of Technology, Perth, WA 6845, Australia
    Chem Biol Drug Des 72:455-82. 2008
    ..New approaches to the development of GAG mimetics as possible new glycotherapeutics are also briefly covered...
  3. ncbi Sanfilippo syndrome: a mini-review
    M J Valstar
    Department of Pediatrics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Inherit Metab Dis 31:240-52. 2008
    ....
  4. ncbi C. elegans Kallmann syndrome protein KAL-1 interacts with syndecan and glypican to regulate neuronal cell migrations
    Martin L Hudson
    Department of Molecular, Cell, and Developmental Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA
    Dev Biol 294:352-65. 2006
    ..SDN-1 and GPN-1 are expressed in ventral neuroblasts and have redundant roles in KAL-1-dependent neuroblast migrations. Our findings suggest that KAL-1 interacts with multiple HSPGs to promote cell migration...
  5. ncbi FGFs, heparan sulfate and FGFRs: complex interactions essential for development
    D M Ornitz
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioessays 22:108-12. 2000
    ..The establishment of a role for heparan sulfate in FGFR activation in vivo suggests that tissue-specific differences in the structure of heparan may modulate the activity of FGF. BioEssays 22:108-112, 2000...
  6. pmc Productive dengue virus infection of human endothelial cells is directed by heparan sulfate-containing proteoglycan receptors
    Nadine Dalrymple
    Department of Molecular Genetics and Microbiology, Stony Brook University, Life Sciences Rm 126, Stony Brook, NY 11794 5222, USA
    J Virol 85:9478-85. 2011
    ....
  7. ncbi Endothelial cell glycocalyx modulates immobilization of leukocytes at the endothelial surface
    Alina A Constantinescu
    Department of Medical Physics, University of Amsterdam, Amsterdam, The Netherlands
    Arterioscler Thromb Vasc Biol 23:1541-7. 2003
    ....
  8. pmc Extracellular sugar modifications provide instructive and cell-specific information for axon-guidance choices
    Hannes E Bülow
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Curr Biol 18:1978-85. 2008
    ....
  9. pmc Perlecan maintains the integrity of cartilage and some basement membranes
    M Costell
    Department of Experimental Pathology, Lund University, S 221 85 Lund, Sweden
    J Cell Biol 147:1109-22. 1999
    ....
  10. ncbi Differential sulfations and epimerization define heparan sulfate specificity in nervous system development
    Hannes E Bülow
    Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY 10032 USA
    Neuron 41:723-36. 2004
    ..Our results demonstrate that the molecular diversity in HS encodes information that is crucial for different aspects of neuronal development...
  11. pmc A systems biology approach for the investigation of the heparin/heparan sulfate interactome
    Alessandro Ori
    Institute of Integrative Biology and Centre for Glycobiology, University of Liverpool, Liverpool, United Kingdom
    J Biol Chem 286:19892-904. 2011
    ..The evolutionary role of HS was reinforced by the presence of a rudimentary HS biosynthetic machinery in a unicellular organism at the root of the metazoan lineage...
  12. ncbi Regulation of Wnt signaling and embryo patterning by an extracellular sulfatase
    G K Dhoot
    Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
    Science 293:1663-6. 2001
    ....
  13. pmc Initial interaction of herpes simplex virus with cells is binding to heparan sulfate
    D WuDunn
    Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637
    J Virol 63:52-8. 1989
    ..Consistent with their broad host ranges, the two HSV serotypes use as primary receptors ubiquitous cell surface components known to participate in interactions with the extracellular matrix and with other cell surfaces...
  14. pmc Efficient infection of cells in culture by type O foot-and-mouth disease virus requires binding to cell surface heparan sulfate
    T Jackson
    Pirbright Laboratory, Institute for Animal Health, Pirbright, Surrey, United Kingdom
    J Virol 70:5282-7. 1996
    ..The results show that entry of type O FMDV into cells is a complex process and suggest that the initial contact with the cell surface is made through heparan sulfate...
  15. ncbi A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry
    D Shukla
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Cell 99:13-22. 1999
    ....
  16. pmc Proteoglycans in health and disease: new concepts for heparanase function in tumor progression and metastasis
    Uri Barash
    Cancer and Vascular Biology Research Center, Rappaport Faculty of Medicine, Haifa, Israel
    FEBS J 277:3890-903. 2010
    ....
  17. ncbi Heparan sulfate-mediated binding of infectious dengue virus type 2 and yellow fever virus
    Raphaele Germi
    Laboratoire de Virologie Moléculaire et Structurale EA 2939, Universite Joseph Fourier, Grenoble, La Tronche, 38700, France
    Virology 292:162-8. 2002
    ..Heparin also inhibited Yellow fever virus infection by 97%. Using infectious virus, the present work shows the importance of heparan sulfate in binding and infection of these two flaviviruses...
  18. pmc Shaping morphogen gradients by proteoglycans
    Dong Yan
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Cold Spring Harb Perspect Biol 1:a002493. 2009
    ..Here, we review these data, highlighting recent findings that reveal mechanistic roles of HSPGs in controlling morphogen gradient formation...
  19. ncbi Shedding of the endothelial glycocalyx in patients undergoing major vascular surgery with global and regional ischemia
    Markus Rehm
    Clinic of Anesthesiology, Ludwig Maximilians University, Marchioninistrasse 15, 81377 Munich, Germany
    Circulation 116:1896-906. 2007
    ..As already demonstrated, this structure plays a key role in the regulation of inflammation and vascular permeability...
  20. ncbi The role of heparan sulphate in inflammation
    Christopher R Parish
    Division of Immunology and Genetics, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia
    Nat Rev Immunol 6:633-43. 2006
    ....
  21. ncbi The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate
    T Lind
    Department of Medical Biochemistry and Microbiology, Uppsala University, The Biomedical Center, Box 575, S 751 23 Uppsala, Sweden
    J Biol Chem 273:26265-8. 1998
    ..Thus at least two members of the EXT family of tumor suppressors encode glycosyltransferases involved in the chain elongation step of HS biosynthesis...
  22. ncbi Heparin and heparan sulfate: structure and function
    Dallas L Rabenstein
    Department of Chemistry, University of California, Riverside 92521, USA
    Nat Prod Rep 19:312-31. 2002
    ..The literature up to August 2001 is reviewed, and 208 references are cited...
  23. pmc Altered heparan sulfate structure in mice with deleted NDST3 gene function
    Srinivas R Pallerla
    Department of General Zoology and Genetics, Westfalische Wilhelms Universitat Munster, Munster, Germany
    J Biol Chem 283:16885-94. 2008
    ....
  24. ncbi Sulfated polysaccharides enhance the biological activities of bone morphogenetic proteins
    Takatora Takada
    Department of Biochemistry, Showa University, Tokyo 142 8555, Japan
    J Biol Chem 278:43229-35. 2003
    ..Taken together, these results suggest that sulfated polysaccharides enhance the biological activity of both homodimers and heterodimers of BMPs by continuously serving the ligands to their signaling receptors expressed on cell membranes...
  25. ncbi Plasma and urinary levels of dermatan sulfate and heparan sulfate derived disaccharides after long-term enzyme replacement therapy (ERT) in MPS I: correlation with the timing of ERT and with total urinary excretion of glycosaminoglycans
    Minke H de Ru
    Department of Pediatrics, Amsterdam Lysosome Centre Sphinx, Academic Medical Center, University Hospital of Amsterdam, Amsterdam, The Netherlands
    J Inherit Metab Dis 36:247-55. 2013
    ..We studied the concentrations of heparan sulfate and dermatan sulfate derived disaccharides (HS and DS, respectively) in the plasma and urine of seven patients and compared these levels with total urinary GAGs (uGAGs) levels...
  26. ncbi Syndecans as receptors and organizers of the extracellular matrix
    Xiaojie Xian
    Department of Biomedical Sciences, University of Copenhagen, Biocenter, Denmark
    Cell Tissue Res 339:31-46. 2010
    ..Developmental deficits in lower vertebrates in which syndecans are eliminated are also informative and suggest that, in mammals, redundancy is a key issue...
  27. pmc Human herpesvirus 8 envelope glycoprotein K8.1A interaction with the target cells involves heparan sulfate
    F Z Wang
    Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Virol 75:7517-27. 2001
    ..These findings suggest that gpK8.1A interaction with target cells involves cell surface HS-like moieties, and HHV-8 interaction with HS could be in part mediated by virion envelope-associated gpK8.1A...
  28. ncbi Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor
    Kenta Okamoto
    Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan
    J Gen Virol 93:761-70. 2012
    ..Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection...
  29. ncbi Interactions between heparan sulfate and proteins-design and functional implications
    Ulf Lindahl
    Department of Medical Biochemistry and Microbiology, University of Uppsala, Uppsala, Sweden
    Int Rev Cell Mol Biol 276:105-59. 2009
    ..These findings raise intriguing questions concerning the functional significance of regulation in HS biosynthesis...
  30. ncbi Perlecan domain V of Drosophila melanogaster. Sequence, recombinant analysis and tissue expression
    M V Friedrich
    Max Planck Institut fur Biochemie, Germany
    Eur J Biochem 267:3149-59. 2000
    ..This indicates, as for mammalian species, a distinct role of perlecan during Drosophila development...
  31. pmc Carrageenan is a potent inhibitor of papillomavirus infection
    Christopher B Buck
    Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland, USA
    PLoS Pathog 2:e69. 2006
    ..Some of these products block HPV infectivity in vitro, even when diluted a million-fold. Clinical trials are needed to determine whether carrageenan-based products are effective as topical microbicides against genital HPVs...
  32. pmc Sézary syndrome cells overexpress syndecan-4 bearing distinct heparan sulfate moieties that suppress T-cell activation by binding DC-HIL and trapping TGF-beta on the cell surface
    Jin Sung Chung
    Department of Dermatology, The University of Texas Southwestern Medical Center and Dermatology Section Medical Service, Dallas Veterans Affairs Medical Center, Dallas, TX 75390 9069, USA
    Blood 117:3382-90. 2011
    ..Finally, we showed toxin-conjugated DC-HIL to abrogate the ability of SS cells to proliferate in vitro. These findings indicate that SD-4 bearing distinct HS moieties plays a pathogenic role in SS and may be targeted for treatment...
  33. ncbi Perlecan is essential for cartilage and cephalic development
    E Arikawa-Hirasawa
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA
    Nat Genet 23:354-8. 1999
    ..Our findings suggest that these molecules affect similar signalling pathways...
  34. ncbi Contribution of EXT1, EXT2, and EXTL3 to heparan sulfate chain elongation
    Marta Busse
    Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway
    J Biol Chem 282:32802-10. 2007
    ..One of the mutations, EXT2-Y419X, resulted in a truncated protein. Interestingly, the capacity of wild type EXT2 to enhance HS chain length together with EXT1 was not shared by the EXT2-Y419X mutant...
  35. ncbi Interactions of fibrillin-1 with heparin/heparan sulfate, implications for microfibrillar assembly
    K Tiedemann
    , , Ratzeburger Allee 160, , Germany
    J Biol Chem 276:36035-42. 2001
    ..These studies suggest that binding of fibrillin-1 to proteoglycan-associated heparan sulfate chains is an important step in the assembly of microfibrils...
  36. ncbi Mapping of the binding of platelet-derived growth factor to distinct domains of the basement membrane proteins BM-40 and perlecan and distinction from the BM-40 collagen-binding epitope
    W Göhring
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Biochem 255:60-6. 1998
    ..Neither nidogen, BM-40 or perlecan domain III-2 interfered with the binding of PDGF to its alpha and beta receptors, however, suggesting that these interactions may be mainly used for storage of PDGF in the extracellular matrix...
  37. ncbi Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein
    Daisuke Kato
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and Global COE Program for Innovation in Human Health Sciences, 52 1 Yada, Suruga ku, Shizuoka shi, Shizuoka 422 8526, Japan
    Antiviral Res 88:236-43. 2010
    ..In conclusion, CSE showed antiviral activity as an entry inhibitor targeting E protein of dengue virus...
  38. pmc Organ-specific sulfation patterns of heparan sulfate generated by extracellular sulfatases Sulf1 and Sulf2 in mice
    Satoshi Nagamine
    Department of Molecular Neurobiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
    J Biol Chem 287:9579-90. 2012
    ..These findings indicate that Sulf1 and Sulf2 differentially contribute to the generation of organ-specific sulfation patterns of heparan sulfate...
  39. doi Changes in glycosaminoglycans and related proteoglycans in intrinsically aged human skin in vivo
    Jang Hee Oh
    Exp Dermatol 20:454-6. 2011
    ..Versican stain was increased in male aged skin, but not in female. Age- and gender-related changes in GAGs and PGs in intrinsically aged buttock skin elucidated in this study may play important roles in intrinsic skin ageing process...
  40. ncbi Generation and characterization of a series of monoclonal antibodies that specifically recognize [HexA(+/-2S)-GlcNAc]n epitopes in heparan sulfate
    Kiyoshi Suzuki
    Central Research Laboratories, Seikagaku Corporation, 3 1253 Tateno, Higashiyamato, Tokyo, 207 0021, Japan
    Glycoconj J 25:703-12. 2008
    ..Furthermore, ACH55 specifically stained Purkinje cells. These results suggest that there is unordinary expression of IdoA2S-GlcNAc and IdoA-GlcNAc in specific parts of the nervous system...
  41. pmc Finding a needle in a haystack: development of a combinatorial virtual screening approach for identifying high specificity heparin/heparan sulfate sequence(s)
    Arjun Raghuraman
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, 23298, USA
    J Med Chem 49:3553-62. 2006
    ....
  42. pmc The anticancer activity of lytic peptides is inhibited by heparan sulfate on the surface of the tumor cells
    Bodil Fadnes
    Department of Medical Biochemistry, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
    BMC Cancer 9:183. 2009
    ..The purpose of this study was to investigate the role of the two GAGs in the cytotoxic activity of CAPs...
  43. ncbi Role of the putative heparan sulfate glycosaminoglycan-binding site of the adenovirus type 5 fiber shaft on liver detargeting and knob-mediated retargeting
    Neus Bayo-Puxan
    Translational Research Laboratory, IDIBELL Institut Català d Oncologia, Av Gran Via s n km 2 7, L Hospitalet de Llobregat, 08907 Barcelona, Spain
    J Gen Virol 87:2487-95. 2006
    ..The insertion of ligands at the hexon or protein IX may be required to benefit from the fiber shaft mutation-detargeting properties...
  44. pmc Modulatory effects of heparin and short-length oligosaccharides of heparin on the metastasis and growth of LMD MDA-MB 231 breast cancer cells in vivo
    P Mellor
    Breast Cancer Research Group, School of Surgical and Reproductive Sciences, Newcastle University, Newcastle upon Tyne, UK
    Br J Cancer 97:761-8. 2007
    ..001) and area of metastases, suggesting a differing mechanism for the action of polymeric and heparin-derived oligosaccharides in the inhibition of tumour growth and metastases...
  45. ncbi The S100 family heterodimer, MRP-8/14, binds with high affinity to heparin and heparan sulfate glycosaminoglycans on endothelial cells
    Matthew J Robinson
    Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund and the in Situ Hybridization Service and Histopathology Unit, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 277:3658-65. 2002
    ..CD36 and RAGE, two other putative receptors for MRP-8/14, were not expressed by HMEC-1 cells. This binding activity may explain the immobilization of the MRP-8/14 complex on endothelium that is observed in vivo...
  46. pmc Heparan sulfate regulates amyloid precursor protein processing by BACE1, the Alzheimer's beta-secretase
    Zoe Scholefield
    School of Biosciences, University of Birmingham, Birmingham, UK
    J Cell Biol 163:97-107. 2003
    ..Furthermore, inhibition of cellular HS synthesis results in enhanced BACE1 activity. Our findings identify HS as a natural regulator of BACE1 and suggest a novel mechanism for control of APP processing...
  47. pmc Heparan sulfate is required for embryonic stem cells to exit from self-renewal
    Daniel C Kraushaar
    Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 285:5907-16. 2010
    ....
  48. ncbi Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state
    Fredrik Lanner
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    Stem Cells 28:191-200. 2010
    ..We conclude that N-sulfated HS is required for FGF4 signaling to maintain ES cells primed for differentiation in a heterogeneous state. Inhibiting this pathway facilitates a more naïve ground state...
  49. pmc Early neurodegeneration progresses independently of microglial activation by heparan sulfate in the brain of mucopolysaccharidosis IIIB mice
    Jérôme Ausseil
    Unité Rétrovirus et Transfert Génétique, INSERM U622, Department of Neuroscience, Institut Pasteur, Paris, France
    PLoS ONE 3:e2296. 2008
    ..We addressed causative links between the biochemical, pathological and inflammatory disorders in a mouse model of this disease...
  50. ncbi Generation and application of type-specific anti-heparan sulfate antibodies using phage display technology. Further evidence for heparan sulfate heterogeneity in the kidney
    T H van Kuppevelt
    Department of Biochemistry, 160, Faculty of Medical Sciences, University of Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    J Biol Chem 273:12960-6. 1998
    ..This is the first time this technique has been successfully applied to obtain directly antibodies to (poly)saccharides...
  51. ncbi Heparanase involvement in physiology and disease
    N J Nasser
    Department of Oncology, Shaare Zedek Medical Center, Jerusalem, Israel
    Cell Mol Life Sci 65:1706-15. 2008
    ..Heparanase overexpression in a variety of malignant tumors suggests that it could be a target for anti-cancer therapy...
  52. ncbi Cell fusion induced by herpes simplex virus glycoproteins gB, gD, and gH-gL requires a gD receptor but not necessarily heparan sulfate
    P E Pertel
    Department of Medicine, Northwestern University Medical School, Chicago, Illinois, 60611, USA
    Virology 279:313-24. 2001
    ..Overall, the viral and cellular determinants of HSV-1-induced cell fusion are similar to those for viral entry, except that HSV-1 entry is significantly enhanced by binding of virus to cell surface heparan sulfate...
  53. ncbi Characterization of growth factor-binding structures in heparin/heparan sulfate using an octasaccharide library
    Satoko Ashikari-Hada
    Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 279:12346-54. 2004
    ..Thus, the library may provide new reagents to specifically regulate bindings of the growth factors to HS...
  54. ncbi Heparanase gene silencing, tumor invasiveness, angiogenesis, and metastasis
    Evgeny Edovitsky
    Department of Oncology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    J Natl Cancer Inst 96:1219-30. 2004
    ..We used gene-silencing strategies to evaluate the role of heparanase in malignancy and to explore the therapeutic potential of its specific targeting...
  55. pmc A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils
    Sara Massena
    Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
    Blood 116:1924-31. 2010
    ..In conclusion, an intravascular chemokine gradient sequestered by endothelial HS effectively directs crawling leukocytes toward transmigration loci close to the infection site...
  56. pmc The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate
    C McCormick
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Proc Natl Acad Sci U S A 97:668-73. 2000
    ..These findings provide a rationale to explain how inherited mutations in either of the two EXT genes can cause loss of activity, resulting in hereditary multiple exostoses...
  57. ncbi Heparan sulfate: anchor for viral intruders?
    D Spillmann
    Department of Medical Biochemistry and Microbiology, The Biomedical Center, Uppsala University, Box 582, 75 123 Uppsala, Sweden
    Biochimie 83:811-7. 2001
    ....
  58. pmc Structural diversity of heparan sulfate binding domains in chemokines
    Hugues Lortat-Jacob
    Institut de Biologie Structurale, Centre National de la Recherche Scientifique Commissariat à l Energie Atomique Université Joseph Fourier, Laboratoire de Biophysique Moleculaire, 41 Rue Horowitz, 38027 Grenoble Cedex 01, France
    Proc Natl Acad Sci U S A 99:1229-34. 2002
    ..Our results help to rationalize how unique sequences of HS specifically bind any given chemokine. The conclusions open the route for a rational design of compounds of therapeutical interest that could influence chemokine activity...
  59. ncbi Lipoprotein-heparan sulfate interactions in the Hh pathway
    Christina Eugster
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Dev Cell 13:57-71. 2007
    ..They further suggest that the complement of proteins present on lipoprotein particles can regulate the activity of morphogens...
  60. pmc Heparan sulfate biosynthesis enzymes EXT1 and EXT2 affect NDST1 expression and heparan sulfate sulfation
    Jenny Presto
    Department of Medical Biochemistry and Microbiology, Biomedical Center, University of Uppsala, Box 582, SE 751 23 Uppsala, Sweden
    Proc Natl Acad Sci U S A 105:4751-6. 2008
    ..Increased NDST activity in fibroblasts with a gene trap mutation in EXT1 supports this notion. These results support a model in which the enzymes of HS biosynthesis form a complex, or a GAGosome...
  61. ncbi Heparin-dependent attachment of respiratory syncytial virus (RSV) to host cells
    T Krusat
    Department of Medical Microbiology and Virology, Ruhr University, Bochum, Germany
    Arch Virol 142:1247-54. 1997
    ..Therefore heparin-like proteoglycans showed properties required for attachment of RSV to host cells...
  62. ncbi A simplified and sensitive fluorescent method for disaccharide analysis of both heparan sulfate and chondroitin/dermatan sulfates from biological samples
    Jon A Deakin
    Cancer Research UK Glyco Oncology Group, School of Cancer and Imaging Sciences, University of Manchester, Paterson Institute for Cancer Research, Manchester M20 4BX, UK
    Glycobiology 18:483-91. 2008
    ..This simplified method should find a widespread utility for analyzing glycosaminoglycans from limited animal and cell culture samples...
  63. pmc Role for 3-O-sulfated heparan sulfate as the receptor for herpes simplex virus type 1 entry into primary human corneal fibroblasts
    Vaibhav Tiwari
    University of Illinois at Chicago, Lions of Illinois Eye Research Institute, M C 648, 1855 West Taylor Street, Chicago, IL 60612, USA
    J Virol 80:8970-80. 2006
    ..Taken together, the data in this report provide novel information on the role of 3-OS HS in mediating infection of CF, a natural target cell type...
  64. ncbi Human herpesvirus 8 envelope-associated glycoprotein B interacts with heparan sulfate-like moieties
    S M Akula
    Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Virology 284:235-49. 2001
    ..These results suggest that gB plays an important role in the infectious process of HHV-8 and virus interaction with cell-surface HS-like moieties could be in part mediated by the envelope-associated gB...
  65. pmc Mouse model of Sanfilippo syndrome type B produced by targeted disruption of the gene encoding alpha-N-acetylglucosaminidase
    H H Li
    Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 96:14505-10. 1999
    ....
  66. ncbi Syndecan-1 and -4 synthesized simultaneously by mouse mammary gland epithelial cells bear heparan sulfate chains that are apparently structurally indistinguishable
    Masahiro Zako
    Division of Newborn Medicine, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 278:13561-9. 2003
    ....
  67. pmc Highly sulfated nonreducing end-derived heparan sulfate domains bind fibroblast growth factor-2 with high affinity and are enriched in biologically active fractions
    Hicham Naimy
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 286:19311-9. 2011
    ..The results suggest a role of the NRE of HS in FGF2 signaling and favor the formation of the symmetrical architecture on short NS domains...
  68. pmc Interaction of Chlamydia trachomatis with mammalian cells is independent of host cell surface heparan sulfate glycosaminoglycans
    Richard S Stephens
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Infect Immun 74:1795-9. 2006
    ..These data do not support the hypothesis and demonstrate that host cell surface heparan sulfate does not serve an essential functional role in chlamydial infectivity...
  69. pmc Human papillomavirus type 31b infection of human keratinocytes does not require heparan sulfate
    Nicole A Patterson
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA
    J Virol 79:6838-47. 2005
    ..These data suggest that HPV31b infection of human keratinocytes, the natural host cell for HPV infections in vivo, does not require a heparan-sulfonated receptor, whereas heparan sulfate is important for infection of some other cells...
  70. pmc Preactivation-based, one-pot combinatorial synthesis of heparin-like hexasaccharides for the analysis of heparin-protein interactions
    Zhen Wang
    Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA
    Chemistry 16:8365-75. 2010
    ..This provides useful information for further development of more potent compounds towards FGF-2 binding, which can have potential applications in wound healing and anticancer therapy...
  71. pmc Heparanase 2 interacts with heparan sulfate with high affinity and inhibits heparanase activity
    Flonia Levy-Adam
    Cancer and Vascular Biology Research Center, Bruce Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel
    J Biol Chem 285:28010-9. 2010
    ..03). Hpa2 appears to restrain tumor metastasis, likely by attenuating heparanase enzymatic activity, conferring a favorable outcome of head and neck cancer patients...
  72. ncbi Heparanase is expressed in osteoblastic cells and stimulates bone formation and bone mass
    Vardit Kram
    Bone Laboratory, The Hebrew University of Jerusalem, Jerusalem, Israel
    J Cell Physiol 207:784-92. 2006
    ..Collectively, our data suggest that proteoglycans tonically suppress osteoblast function and that this inhibition is alleviated by HS degradation with heparanase...
  73. ncbi Molecular genetics of mucopolysaccharidosis type IIIA and IIIB: Diagnostic, clinical, and biological implications
    G Yogalingam
    Lysosomal Diseases Research Unit, Department of Chemical Pathology, Women s and Children s Hospital, North Adelaide, South Australia, Australia
    Hum Mutat 18:264-81. 2001
    ..However, molecular characterization of MPS-IIIA patients has revealed a high incidence of particular mutations of different geographical origins, which will be beneficial for the molecular diagnosis of MPS-IIIA...
  74. pmc N-sulfation of heparan sulfate regulates early branching events in the developing mammary gland
    Kevin T Bush
    Department of Pediatrics, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 287:42064-70. 2012
    ..Together with previous work, the data indicate that different stages of ductal branching and lobuloalveolar formation are regulated by distinct sets of heparan sulfate biosynthetic enzymes in an appropriate growth factor context...
  75. pmc Inhibitory peptides of the sulfotransferase domain of the heparan sulfate enzyme, N-deacetylase-N-sulfotransferase-1
    Tarsis F Gesteira
    Departamentos de Bioquímica, Universidade Federal de Sa o Paulo, 04044 020 Sa o Paulo, Brazil
    J Biol Chem 286:5338-46. 2011
    ..The discovery of inhibitory peptides in this way suggests a method for developing peptide inhibitors of heparan sulfate biosynthesis...
  76. pmc Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis
    Sassan HajMohammadi
    Section of Cardiology, Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire, USA
    J Clin Invest 111:989-99. 2003
    ..Instead, 3-OST-1-deficient mice exhibited unanticipated phenotypes suggesting that HS(act) or additional 3-OST-1-derived structures may serve alternate biologic roles...
  77. ncbi Chondroitin sulfate characterized by the E-disaccharide unit is a potent inhibitor of herpes simplex virus infectivity and provides the virus binding sites on gro2C cells
    Kicki Bergefall
    Department of Clinical Virology, Goteborg University, Sweden
    J Biol Chem 280:32193-9. 2005
    ..Knowledge of the determinants of antiviral properties of CS-E will help in the development of inhibitors of herpes simplex virus infections in humans...
  78. pmc Portable sulphotransferase domain determines sequence specificity of heparan sulphate 3-O-sulphotransferases
    T Yabe
    Angiogenesis Research Center, Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Biochem J 359:235-41. 2001
    ..Thus sequence-specific properties of 3-OSTs are defined by a self-contained sulphotransferase domain and are not directly influenced by the divergent N-terminal region...
  79. pmc Enzyme interactions in heparan sulfate biosynthesis: uronosyl 5-epimerase and 2-O-sulfotransferase interact in vivo
    M A Pinhal
    Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0687, USA
    Proc Natl Acad Sci U S A 98:12984-9. 2001
    ..These findings provide in vivo evidence for the formation of complexes among enzymes involved in heparan sulfate biosynthesis. The functional significance of these complexes may relate to the rapidity of heparan sulfate formation...
  80. ncbi Tinkering with heparan sulfate sulfation to steer development
    Bushra Gorsi
    Division of Cardiac and Endocrine Sciences, University of Manchester, UK, M13 9NT
    Trends Cell Biol 17:173-7. 2007
    ..In addition, the latest discovery of HS acting enzymes, the Sulfs, responsible for extracellular tweaking of HS sulfation levels subsequent to biosynthesis will be considered...
  81. ncbi Endogenous heparan sulfate and heparin modulate bone morphogenetic protein-4 signaling and activity
    Shaukat A Khan
    Hematology and Oncology Section, Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Am J Physiol Cell Physiol 294:C1387-97. 2008
    ....
  82. pmc Direct visualization of specifically modified extracellular glycans in living animals
    Matthew Attreed
    Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, USA
    Nat Methods 9:477-9. 2012
    ..This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo...
  83. pmc Cell entry and trafficking of human adenovirus bound to blood factor X is determined by the fiber serotype and not hexon:heparan sulfate interaction
    Stephanie Corjon
    University Lyon 1, Inra UMR 754, Retrovirus and Comparative Pathology, Lyon, France
    PLoS ONE 6:e18205. 2011
    ....
  84. ncbi A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1
    Vaibhav Tiwari
    Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA
    J Gen Virol 85:805-9. 2004
    ..Taken together, these results indicate that 3-OS HS can play a crucial role in virus entry and cell fusion...
  85. pmc Transforming growth factor-beta1 induces heparan sulfate 6-O-endosulfatase 1 expression in vitro and in vivo
    Xinping Yue
    Section of Pulmonary Diseases, Critical Care and Environmental Medicine, Department of Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    J Biol Chem 283:20397-407. 2008
    ..Taken together, our data indicate that Sulf1 is a TGF-beta1-responsive gene both in vitro and in vivo and may function as a negative regulator of TGF-beta1-induced fibrogenesis...
  86. ncbi Molecular modeling of the interaction between heparan sulfate and cellular growth factors: bringing pieces together
    Nicolas Sapay
    Centre de Recherches sur les Macromolecules Vegetales CNRS, 601 rue de la Chimie, BP 53, 38041 Grenoble Cedex 9, France
    Glycobiology 21:1181-93. 2011
    ..This approach shed light on the plasticity of the growth factors interacting with heparan sulfate. This approach can be extended to the study of other protein/glycosaminoglycan complexes...
  87. pmc Lack of heparan sulfate expression in B-cell lines: implications for Kaposi's sarcoma-associated herpesvirus and murine gammaherpesvirus 68 infections
    Nadine Jarousse
    Department of Molecular and Cell Biology, University of California, 142 Life Sciences Addition, Berkeley, CA 94720, USA
    J Virol 82:12591-7. 2008
    ..Thus, our results indicate that MHV68 and KSHV cycles are blocked in B-cell lines at the binding step due to a lack of surface HS...
  88. ncbi Sustained release and osteogenic potential of heparan sulfate-doped fibrin glue scaffolds within a rat cranial model
    Maria Ann Woodruff
    Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore
    J Mol Histol 38:425-33. 2007
    ..These results further emphasize the important role HS plays in augmenting wound healing, and its successful delivery in a hydrogel provides a novel alternative to autologous bone graft and growth factor-based therapies...
  89. ncbi Heparin, heparan sulfate and heparanase in cancer: remedy for metastasis?
    Jin ping Li
    Department of Medical Biochemistry and Microbiology, The Biomedical Center, Box 582, S 75123, Uppsala University, Uppsala, Sweden
    Anticancer Agents Med Chem 8:64-76. 2008
    ..Understanding the functional roles and the corresponding molecular mechanisms of heparin, heparan sulfate and heparanase in cancer development may pave the way for exploring remedies against tumor metastasis...
  90. pmc The 3'-phosphoadenosine 5'-phosphosulfate transporters, PAPST1 and 2, contribute to the maintenance and differentiation of mouse embryonic stem cells
    Norihiko Sasaki
    Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan
    PLoS ONE 4:e8262. 2009
    ..We propose that PAPST-dependent sulfation of HS or CS chains, which is regulated developmentally, regulates the extrinsic signaling required for the maintenance and normal differentiation of mESCs...
  91. ncbi Membrane-type matrix metalloproteinases 1 and 2 exhibit broad-spectrum proteolytic capacities comparable to many matrix metalloproteinases
    M P d'Ortho
    INSERM U296, Faculte de Medecine, Creteil, France
    Eur J Biochem 250:751-7. 1997
    ..Their location at the surface of cells implies that they could play a significant role in the modulation of cell-matrix interactions...
  92. ncbi Possible existence of common internalization mechanisms among arginine-rich peptides
    Tomoki Suzuki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 277:2437-43. 2002
    ..These results strongly suggest that the arginine-rich basic peptides share a certain part of the internalization pathway...
  93. pmc Composition of the endothelial glycocalyx and its relation to its thickness and diffusion of small solutes
    Lujia Gao
    Department of Bioengineering, The Pennsylvania State University, University Park, PA 16802, USA
    Microvasc Res 80:394-401. 2010
    ..This behavior suggests that the removal of heparan sulfate may cause a collapse of the glycocalyx which counters decreases in thickness by compacting the layer to maintain a constant resistance to filtration...
  94. ncbi Validation of a heparan sulfate-derived disaccharide as a marker of accumulation in murine mucopolysaccharidosis type IIIA
    Barbara King
    Lysosomal Diseases Research Unit, Department of Genetic Medicine, Children, Youth and Women s Health Service, 72 King William Road, North Adelaide SA, Australia
    Mol Genet Metab 87:107-12. 2006
    ..This finding therefore provides evidence for the use of the disaccharide HNS-UA to monitor the effect of therapies for this condition in humans, when treatment strategies are devised...
  95. ncbi Glycosaminoglycans as regulators of stem cell differentiation
    Raymond A A Smith
    Stem Cell Glycobiology Group, Materials Science, University of Manchester, Manchester, M1 7HS, UK
    Biochem Soc Trans 39:383-7. 2011
    ..We predict that this will allow us to control ES cell pluripotency and differentiation in a three-dimensional setting, allowing the generation of differentiated cell types for use in drug discovery/testing or in therapeutics...
  96. pmc Selective filtering of particles by the extracellular matrix: an electrostatic bandpass
    Oliver Lieleg
    Lehrstuhl für Zellbiophysik E27, Technische Universitat Munchen, Garching, Germany
    Biophys J 97:1569-77. 2009
    ..g., the mucus layer of endothelial cells or the hydrogel in the nuclear core complex...
  97. ncbi Comparable stabilisation, structural changes and activities can be induced in FGF by a variety of HS and non-GAG analogues: implications for sequence-activity relationships
    Timothy R Rudd
    School of Biological Sciences, University of Liverpool, Liverpool, L69 7ZB, Great Britain
    Org Biomol Chem 8:5390-7. 2010
    ....
  98. pmc Structure and epitope distribution of heparan sulfate is disrupted in experimental lung hypoplasia: a glycobiological epigenetic cause for malformation?
    Sophie M Thompson
    Institute of Integrative Biology, University of Liverpool, Liverpool, UK
    BMC Dev Biol 11:38. 2011
    ....
  99. pmc Mucopolysaccharidosis type I, unique structure of accumulated heparan sulfate and increased N-sulfotransferase activity in mice lacking α-l-iduronidase
    Rebecca J Holley
    Stem Cell Glycobiology, School of Materials, The University of Manchester, M13 9PL Manchester, UK
    J Biol Chem 286:37515-24. 2011
    ..This mechanism may have severe implications during disease progression but, now identified, could help direct improved therapeutic strategies...
  100. ncbi Dual role of the fringe connection gene in both heparan sulphate and fringe-dependent signalling events
    E M Selva
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 3:809-15. 2001
    ..Embryos with the frc mutation display defects in Wingless, Hedgehog and fibroblast growth factor signalling. Clonal analysis shows that fringe-dependent Notch signalling is disrupted in frc mutant tissue...
  101. ncbi Heterogeneity of heparan sulfates in human lung
    Nicole C Smits
    Department of Biochemistry, University Medical Center Nijmegen, Nijmegen Center for Molecular Life Sciences, Nijmegen, 6500 HB Nijmegen, The Netherlands
    Am J Respir Cell Mol Biol 30:166-73. 2004
    ..Taken together, these data suggest a specific role for HS epitopes in human lung. The antibodies obtained may be valuable tools to study HS in pulmonary diseases...

Research Grants65

  1. The Glycocalyx and Endothelial Barrier Regulation
    RANDAL DULL; Fiscal Year: 2005
    ..This K08 will provide the foundation for a research career dedicated to understanding the molecular mechanisms of endothelial barrier regulation. ..
  2. Strategy for Therapy of the Pseudoachondroplasia (PSACH) Phenotype: Understanding
    Jacqueline Hecht; Fiscal Year: 2006
    ..abstract_text> ..
  3. Correction of neural abnormalities in Hurler syndrome
    Pankaj Gupta; Fiscal Year: 2003
    ....
  4. Correction of Hurler syndrome by multipotent stem cells
    Pankaj Gupta; Fiscal Year: 2007
    ..If MAPC transplantation is of benefit in the Hurler model, similar strategies may be developed for treating diverse neurodegenerative disorders. ..
  5. PATHOBIOCHEMISTRY OF PROTEOGLYCANS IN DEVELOPMENT
    Scott Saunders; Fiscal Year: 2009
    ..abstract_text> ..
  6. Genetic Studies of Clubfoot (ITEV)
    Jacqueline T Hecht; Fiscal Year: 2010
    ..Identification of high-risk genotypes can lead to the development of prevention programs in selected populations and may suggest gene-based prevention strategies. ..
  7. Microenvironment Modulates HIV Replication in Macrophage
    Philippe Gallay; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  8. BIOLOGY OF BAMACAN IN TUMORIGENESIS
    Giancarlo Ghiselli; Fiscal Year: 2006
    ..3) The contribution of bamacan in vivo to colon tumorigenesis by generating transgenic mice over-expressing bamacan in the intestinal epithelium. ..
  9. 2006 Proteoglycans Gordon Research Conferences
    ALAN RAPRAEGER; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  10. Heparan sulfate in neural development
    Yu Yamaguchi; Fiscal Year: 2007
    ..We will perform histological, cell biological, and physiological analyses to determine the physiological role of HS in CNS synapses. ..
  11. Oligosaccharides Regulating Cell Function
    Ram Sasisekharan; Fiscal Year: 2006
    ..This grant application represents a high risk/high reward undertaking aimed at developing LMP as an effective treatment for of debilitating diseases caused by the deregulation of apoptosis, such as cancer. [unreadable] [unreadable]..
  12. Structural Specificity of Heparan Sulfate for Herpes Infections
    JIAN NONE LIU; Fiscal Year: 2010
    ..Our results could lead to a novel approach to treat herpes simplex virus infections and improve the basic understanding on the biosynthesis of HS with specific sulfonation patterns. ..
  13. MICROBIOLOGY OF CHLAMYDIA TRACHOMATIS ATTACHMENT
    Richard Stephens; Fiscal Year: 2005
    ....
  14. Molecular Principles of FGF-HSGAG Interactions
    Ram Sasisekharan; Fiscal Year: 2006
    ..abstract_text> ..
  15. Proteolytic cleavage of the Hendra virus fusion protein
    REBECCA DUTCH; Fiscal Year: 2006
    ..abstract_text> ..
  16. Role of Inter-alpha Inhibitors in Anthrax Intoxication
    Steven Opal; Fiscal Year: 2007
    ..Our research is focused on the development of a new and safe treatment based on the ability of inter-alpha proteins to prevent the fatal consequences of anthrax infection. [unreadable] [unreadable] [unreadable] [unreadable]..
  17. CCNY/MSKCC BIOMEDICAL ENGINEERING PARTNERSHIP
    JOHN TARBELL; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  18. ENZYMATIC DEGRADATION OF GLYCOSAMINOGLYCANS
    Ram Sasisekharan; Fiscal Year: 2010
    ..We believe that our study would truly accelerate the progress of understanding GAG-structure-function relationships in fundamental biological processes-a research area that is gaining increasing importance. ..
  19. SHEAR STRESS EFFECTS ON ENDOTHELIAL TRANSPORT
    John M Tarbell; Fiscal Year: 2010
    ..abstract_text> ..
  20. Structure of Chlamydia-Specific Host Cell Vacuoles
    Richard S Stephens; Fiscal Year: 2010
    ..This research will establish the mechanism of microbial survival in cells that will serve as targets for prevention and control of chlamydial infection. ..
  21. Regulation of angiogenesis by syndecan-1
    ALAN RAPRAEGER; Fiscal Year: 2009
    ....
  22. Glycoside Inhibitors of Tumor Metastasis
    Jeffrey Esko; Fiscal Year: 2009
    ..Solid tumor formation and inflammatory reactions will be analyzed to confirm the selective inhibitory action of the analog on tumor metastasis. ..
  23. HMPV Fusion protein-host cell interactions
    REBECCA DUTCH; Fiscal Year: 2008
    ..This research will address significant questions concerning the cellular factors which interact with the HMPV fusion protein to facilitate attachment of the virus and entry into cells. [unreadable] [unreadable] [unreadable]..
  24. PROTEOGLYCANS OF THE PLASMA MEMBRANE
    ALAN RAPRAEGER; Fiscal Year: 2008
    ..We anticipate that completion of these studies will provide new and unexpected insights into the function of this receptor, and will lead to the development of new cancer treatments. ..
  25. Growth Factors in Vascular Biology
    MATTHEW NUGENT; Fiscal Year: 2008
    ..These studies will have wide ranging impact on vascular biology and will likely reveal previously undefined mechanisms of growth factor control that could help identify targets for clinical modulation. ..
  26. Structure-Activity Relationships of LMWHs
    Ram Sasisekharan; Fiscal Year: 2008
    ..anti-Xa and anti-IIa and their pharmacokinetic in vivo elimination ..
  27. Design of Gene Delivery System to Target Hepatocytes
    Mark Davis; Fiscal Year: 2008
    ..The long-term objective of our proposal is to design and engineer a generalized, synthetic system for ultimately delivering nucleic acid-based therapeutics to hepatocytes in humans. ..
  28. Syndecan Agonists and Antagonists as Microbicides
    Philippe Gallay; Fiscal Year: 2007
    ..Thus, microbicides targeting gp120-syndecan interactions will exhibit a broad inhibitory spectrum against sexually transmitted pathogens. [unreadable] [unreadable] [unreadable]..
  29. Heparan Sulfate Proteoglycans and HIV Pathogenesis
    Philippe Gallay; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  30. Database for Glycan Structures
    Ram Sasisekharan; Fiscal Year: 2005
    ..This forms the framework for the present STTR grant application as a fast-track Phases I and II grant application that is highly integrated towards addressing the requirements of the glycomics field. ..
  31. Cellular Microbiology of Chlamydia pneumoniae
    Richard Stephens; Fiscal Year: 2005
    ..pneumoniae during persistent infection and during growth in different cell types. 4) Identify chlamydial products and test persistently-expressed C. pneumoniae gene products for their ability to induce host cell responses. ..
  32. LASER DOPPLER VELOCIMETER SYSTEM FOR BIOMEDICAL STUDIES
    JOHN TARBELL; Fiscal Year: 2001
    ..The system will be the primary measurement system for the ongoing research in cardiovascular prosthetics including mechanical heart valve cavitation and ventricular assist devices. ..
  33. PROTEOGLYCANS AND BRAIN DEVELOPMENT
    ARTHUR LANDER; Fiscal Year: 2004
    ..These studies should provide insights into how an important and poorly understood class of molecules regulates basic developmental processes, particularly in the nervous system. ..
  34. A POXVIRAL PROTEIN THAT INHIBITS CHEMOKINE FUNCTION
    Mitchell Krathwohl; Fiscal Year: 2003
    ..4) To determine the regions of MC148R that are important for blocking chemotaxis by constructing mutant proteins at both the amino terminus and the internal heparin binding site. ..
  35. HEPARIN-BINDING PEPTIDES FROM CELL ADHESION PROTEINS
    DALLAS RABENSTEIN; Fiscal Year: 2003
    ..These objectives will be achieved using information obtained from high field nuclear magnetic resonance (NMR) spectroscopy experiments. ..
  36. PROTEOGLYCANS OF THE PLASMA MEMBRANE
    ALAN RAPRAEGER; Fiscal Year: 2003
    ..Results will lead to a better understanding of epithelial morphogenesis and to the mechanisms that regulate tumor cell metastasis and invasion. ..
  37. Collagen-binding proteins in softening of uterine cervix
    Robert Kokenyesi; Fiscal Year: 2003
    ....
  38. CHARACTERIZATION OF FRACTALKINE IN VITRO AND IN VIVO
    Lili Feng; Fiscal Year: 2003
    ....
  39. RETROGRADE SIGNALING IN NERVE TERMINAL FORMATION
    HSIAO MING PENG; Fiscal Year: 2002
    ..The principles garnered from this study should lead to a better understanding of the causes of these maladies. ..
  40. BRAIN EXTRACELLULAR MATRIX--ROLES IN NEURAL FUNCTION AND
    Yu Yamaguchi; Fiscal Year: 2002
    ....
  41. STERIC-STABILIZED LIPOSOMES FOR SYSTEMIC GENE DELIVERY
    KENNETH LONGMUIR; Fiscal Year: 2002
    ..2) Gene expression, pharmokinetics, and biodistribution will be assessed for prolonged circulation times. 3) Organ tissues will be examined histologically to assess gene expression in cell types outside the vasculature. ..
  42. GORDON RESEARCH CONFERENCE ON PROTEOGLYCANS
    ARTHUR LANDER; Fiscal Year: 2002
    ....
  43. FUNCTIONAL ANALYSIS OF GLYPICANS IN BRAIN DEVELOPMENT
    Yu Yamaguchi; Fiscal Year: 2001
    ..These studies not only provide new insight into the mechanism of neurogenesis but may shed light on the pathogenesis of congenital brain malformations that may derive from defective proliferation of neural precursor cells. ..
  44. Heparin like Glycosaminoglycan Oligosaccharides
    Ram Sasisekharan; Fiscal Year: 2004
    ..Similar technologies for DNA and proteins include things like gene chips that have revolutionized the study of these molecules. We hope advancements, like out technology, will do the same thing for HLGAGs. ..
  45. INTL CONG OF VIROLOGY, SAN FRANCISCO, ASV TRAVEL REQUEST
    PATRICIA SPEAR; Fiscal Year: 2005
    ....
  46. Role of Heparan Sulfate in FGF Action
    ALAN RAPRAEGER; Fiscal Year: 2005
    ..The long term outcome of this work will be left the derivation of chemical mimetics that can be used as therapeutics in the treatment of cancer and the prevention of birth defects. ..
  47. Role of hyaluronan in developing and adult CNS
    Yu Yamaguchi; Fiscal Year: 2005
    ..The insight derived from these studies could lead to the development of hyaluronan based therapeutic approaches for neurodegenerative and demyelinating diseases. ..
  48. MIDCAREER INVESTIGATOR AWARD IN CEREBROVASCULAR RESEARCH
    Larry Goldstein; Fiscal Year: 2004
    ....
  49. FMRI in Pre-Symptomatic PS1-related Alzheimer's Disease
    John Ringman; Fiscal Year: 2005
    ..UCI, with its strong dementia research program, growing MRI capabilities and opportunity to collaborate at the nearby UCLA Brain Imaging Center, is an excellent environment for this endeavor. ..
  50. Treatment of Hyperglycemia Ischemic Stroke(THIS)Trial
    Askiel Bruno; Fiscal Year: 2004
    ..Additional measurements will include vital signs, serum electrolytes, hemoglobin A-1C, and a global test for multiple clinical outcomes at 3 months. ..
  51. OPTIMIZING MANAGEMENT OF TRANSIENT ISCHEMIC ATTACKS
    S Johnston; Fiscal Year: 2005
    ..This research will provide important results for clinicians and policy makers, and will foster the applicant's development as a health services researcher in cerebrovascular disease. ..
  52. Inhibitors of HIV-Dendritic Cell Interactions as Microbicides
    Philippe Gallay; Fiscal Year: 2008
    ..This approach may not only provide valuable novel microbicides, but it will also allow us to assess the contribution of LC and DC to HIV transmission. ..
  53. Role of heparanase in osteolytic bone metastasis
    Ralph Sanderson; Fiscal Year: 2007
    ..abstract_text> ..
  54. CELLULAR MECHANISM OF DIABETIC NEPHROPATHY
    Lili Feng; Fiscal Year: 2004
    ..Insights gained in the proposed studies will lead to the development of new therapeutic strategies to this life-threatening disease. ..
  55. Herpes simplex virus receptors and signal transduction
    PATRICIA SPEAR; Fiscal Year: 2003
    ..The studies described in this proposal are distinct from those supported by the parent grant but address questions that arise from findings made in the parent project. ..
  56. ENTRY OF HERPES SIMPLEX VIRUS INTO CELLS
    PATRICIA SPEAR; Fiscal Year: 2008
    ..abstract_text> ..
  57. Bioengineering for In Vivo-Viral Gene Delivery
    KENNETH LONGMUIR; Fiscal Year: 2007
    ..The development of this technology will have broad implications for the controlled self-assembly of a wide range of nanoparticle systems, particularly those intended for in vivo drug delivery. [unreadable] [unreadable]..
  58. SYNDECAN FUNCTION ON MYELOMA CELLS
    Ralph Sanderson; Fiscal Year: 2004
    ..Insight gained from these studies will be used to design new therapeutic strategies aimed at halting tumor growth and metastasis. ..
  59. An Artificial Golgi: Controlled GAG Synthesis and Screening
    ROBERT J contact LINHARDT; Fiscal Year: 2010
    ..This artificial Golgi uses a digital microfluidic platform for synthesis and screen activity on a cell microarray to develop a structure activity relationship for fibroblast growth factor signaling. ..
  60. Mechanisms of Nucleic Acid Uptake and The Role of cMDH
    Basil Hanss; Fiscal Year: 2008
    ..2) To determine the functional role of cytosolic malatedehydrogenase in nucleic acid transport; 3) To establish the topology of cMDH relative to plasma membrane. ..
  61. Glycosaminoglycan-Protein Interactions
    Robert Linhardt; Fiscal Year: 2003
    ....
  62. Heparin Glycan Microarray for Screening
    Robert Linhardt; Fiscal Year: 2008
    ..A phase II proposal will be prepared to develop these commercial applications. [unreadable] [unreadable] [unreadable]..
  63. Innate immune evasion and/or antagonism by eastern equine encephalitis virus
    KATHERINE RYMAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..