agar

Summary

Summary: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.

Top Publications

  1. pmc Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus
    John D Perry
    Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, Tyne and Wear, United Kingdom
    J Clin Microbiol 42:4519-23. 2004
  2. ncbi Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
    Irith Wiegand
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, British Columbia, V6T 1Z4, Canada
    Nat Protoc 3:163-75. 2008
  3. pmc Comparison of the quantitative formalin ethyl acetate concentration technique and agar plate culture for diagnosis of human strongyloidiasis
    P M Intapan
    Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
    J Clin Microbiol 43:1932-3. 2005
  4. pmc Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities
    D M Wolk
    Southern Arizona VA Health Care System, Tucson, Arizona, USA
    J Clin Microbiol 47:3933-6. 2009
  5. ncbi Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture
    S Jongwutiwes
    Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 93:398-400. 1999
  6. pmc Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells
    Colin J Ingham
    Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, s Hertogenbosh, The Netherlands
    BMC Microbiol 8:36. 2008
  7. ncbi An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study
    Ieong Wong
    Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA
    Lab Chip 10:2710-9. 2010
  8. pmc Automated counting of bacterial colony forming units on agar plates
    Silvio D Brugger
    Institute for Infectious Diseases, University of Bern, Bern, Switzerland
    PLoS ONE 7:e33695. 2012
  9. ncbi Coculture of spermatogonia with somatic cells in a novel three-dimensional soft-agar-culture-system
    Jan Bernd Stukenborg
    Institute of Reproductive Medicine of the University, Domagkstrasse 11, 48129 Munster, Germany
    J Androl 29:312-29. 2008
  10. ncbi Cytotoxicity of dental alloys, metals, and ceramics assessed by millipore filter, agar overlay, and MTT tests
    G Sjögren
    NIOM Scandinavian Institute of Dental Materials, Haslum, Norway
    J Prosthet Dent 84:229-36. 2000

Detail Information

Publications276 found, 100 shown here

  1. pmc Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus
    John D Perry
    Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, Tyne and Wear, United Kingdom
    J Clin Microbiol 42:4519-23. 2004
    We describe here the development and evaluation of MRSA ID, a new chromogenic agar medium for the specific isolation and identification of methicillin-resistant Staphylococcus aureus (MRSA). We used S...
  2. ncbi Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
    Irith Wiegand
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, British Columbia, V6T 1Z4, Canada
    Nat Protoc 3:163-75. 2008
    The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the ..
  3. pmc Comparison of the quantitative formalin ethyl acetate concentration technique and agar plate culture for diagnosis of human strongyloidiasis
    P M Intapan
    Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
    J Clin Microbiol 43:1932-3. 2005
    The quantitative formalin ethyl acetate concentration technique (QFEC) was compared to agar plate culture (APC) for the detection of Strongyloides stercoralis larvae...
  4. pmc Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities
    D M Wolk
    Southern Arizona VA Health Care System, Tucson, Arizona, USA
    J Clin Microbiol 47:3933-6. 2009
    ..To assess the performance of common methods, the first comparison of MRSASelect agar (MS) and CHROMagar MRSA (CA), with and without broth enrichment followed by a 24-h subculture to MS, was performed...
  5. ncbi Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture
    S Jongwutiwes
    Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 93:398-400. 1999
    The efficacy of agar-plate culture has been evaluated for the detection of Strongyloides stercoralis and hookworm, compared with direct smear, the formalin-ether sedimentation technique and the filter-paper method...
  6. pmc Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells
    Colin J Ingham
    Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, s Hertogenbosh, The Netherlands
    BMC Microbiol 8:36. 2008
    ..The Gram-positive bacterium Paenibacillus vortex exhibits advanced cooperative motility on agar plates resulting in intricate colonial patterns with geometries that are highly sensitive to the environment...
  7. ncbi An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study
    Ieong Wong
    Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA
    Lab Chip 10:2710-9. 2010
    ..The design highlights a simple device-assembly process between PDMS microchannel and agar membrane through conformal contact, and can be easily adapted by microbiologists for their routine laboratory use...
  8. pmc Automated counting of bacterial colony forming units on agar plates
    Silvio D Brugger
    Institute for Infectious Diseases, University of Bern, Bern, Switzerland
    PLoS ONE 7:e33695. 2012
    Manual counting of bacterial colony forming units (CFUs) on agar plates is laborious and error-prone...
  9. ncbi Coculture of spermatogonia with somatic cells in a novel three-dimensional soft-agar-culture-system
    Jan Bernd Stukenborg
    Institute of Reproductive Medicine of the University, Domagkstrasse 11, 48129 Munster, Germany
    J Androl 29:312-29. 2008
    ..to isolate SSCs and to explore their expansion and differentiation potential in a novel three-dimensional Soft-Agar-Culture-System (SACS)...
  10. ncbi Cytotoxicity of dental alloys, metals, and ceramics assessed by millipore filter, agar overlay, and MTT tests
    G Sjögren
    NIOM Scandinavian Institute of Dental Materials, Haslum, Norway
    J Prosthet Dent 84:229-36. 2000
    ..Biocompatibility of dental materials is dependent on the release of elements from the materials. In addition, the composition, pretreatment, and handling of the materials influence the element release...
  11. pmc Selection of brain metastasis-initiating breast cancer cells determined by growth on hard agar
    Lixia Guo
    Department of Cancer Biology Cancer Metastasis Research Center, The University of Texas M D Anderson Cancer Center, Houston, Texas, USA
    Am J Pathol 178:2357-66. 2011
    ..Here, we demonstrate that plating GI-101A human breast cancer cells on hard (0.9%) agar selects for the subpopulation of metastasis-initiating cells...
  12. ncbi In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds
    Priya Ramnani
    Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK
    Anaerobe 18:1-6. 2012
    Fermentation properties and prebiotic potential of novel low molecular weight polysaccharides (LMWPs) derived from agar and alginate bearing seaweeds was investigated...
  13. pmc Evaluation of blood agar microtiter plates for culturing leishmania parasites to titrate parasite burden in spleen and peripheral blood of patients with visceral leishmaniasis
    Radheshyam Maurya
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    J Clin Microbiol 48:1932-4. 2010
    Serial dilution of blood and spleen biopsy specimens, plated on Novy-MacNeal-Nicolle (NNN) blood agar using microtiter culture plates, is a sensitive and reproducible method for detection and growth of Leishmania parasites...
  14. ncbi Validation of a quantitative real-time PCR assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model
    T W Ammann
    Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland
    J Periodontal Res 48:517-26. 2013
    ..PCR (qPCR) assay to quantify the bacteria used in our 10-species in vitro 'subgingival' biofilm model and to compare the quantitative outcome with fluorescence microscopy and colony-forming unit (CFU) counts on selective agar plates.
  15. ncbi Toxicity and bioavailability of copper nanoparticles to the terrestrial plants mung bean (Phaseolus radiatus) and wheat (Triticum aestivum): plant agar test for water-insoluble nanoparticles
    Woo Mi Lee
    Department of Environmental Science, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul 143 701, Korea
    Environ Toxicol Chem 27:1915-21. 2008
    ..The present study demonstrated a plant agar test for homogeneous exposure of nanoparticles to plant species...
  16. pmc Colonyzer: automated quantification of micro-organism growth characteristics on solid agar
    Conor Lawless
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle Upon Tyne NE45PL, UK
    BMC Bioinformatics 11:287. 2010
    High-throughput screens comparing growth rates of arrays of distinct micro-organism cultures on solid agar are useful, rapid methods of quantifying genetic interactions...
  17. ncbi Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay
    Paiboon Sithithaworn
    Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
    Trans R Soc Trop Med Hyg 97:398-402. 2003
    Cross-sectional surveys of parasitic infection were performed using the agar plate culture technique (APCT) and modified formalin-ethyl acetate concentration technique (MFECT) to assess the true prevalence of Strongyloides stercoralis ..
  18. pmc The stringent response is essential for Pseudomonas aeruginosa virulence in the rat lung agar bead and Drosophila melanogaster feeding models of infection
    Stefanie L Vogt
    Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
    Infect Immun 79:4094-104. 2011
    ..melanogaster and was also avirulent in the rat lung agar bead model of infection, a model in which the relA mutant is fully virulent...
  19. ncbi Agar culture of Piscirickettsia salmonis, a serious pathogen of farmed salmonid and marine fish
    Jarle Mikalsen
    National Veterinary Institute, Oslo, Norway
    FEMS Microbiol Lett 278:43-7. 2008
    ..fish, previously considered culturable only in eukaryotic cell-culture systems, was grown for the first time on agar and broth containing enhanced levels of cysteine, thus greatly increasing the potential for isolation, in vitro ..
  20. pmc Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate
    Z Zhong
    Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0322, USA
    Appl Environ Microbiol 67:5771-9. 2001
    An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15...
  21. pmc Comparative study for determination of Mycobacterium tuberculosis susceptibility to first- and second-line antituberculosis drugs by the Etest using 7H11, blood, and chocolate agar
    Ahmet Yilmaz Coban
    Department of Microbiology and Clinical Microbiology, Ondokuz Mayis University Medical School, Samsun, Turkey
    J Clin Microbiol 46:4095-8. 2008
    We investigated the performance of blood and chocolate agar as alternatives to Middlebrook 7H11 agar for testing the susceptibility of Mycobacterium tuberculosis to first-and second-line drugs by the Etest method...
  22. pmc Dynamics of bacterial swarming
    Nicholas C Darnton
    Rowland Institute at Harvard University, Cambridge, Massachusetts, USA
    Biophys J 98:2082-90. 2010
    When vegetative bacteria that can swim are grown in a rich medium on an agar surface, they become multinucleate, elongate, synthesize large numbers of flagella, produce wetting agents, and move across the surface in coordinated packs: ..
  23. ncbi Evaluation of a cefoxitin 30 microg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus
    R Skov
    National Center for Antimicrobials and Infection Control, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    J Antimicrob Chemother 52:204-7. 2003
    To evaluate the performance of a cefoxitin 30 microg disc on Iso-Sensitest agar, using a semi-confluent inoculum and overnight incubation at 35-36 degrees C, for detection of methicillin-resistant Staphylococcus aureus (MRSA).
  24. ncbi Isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 from ground beef using modified rainbow agar and post-immunomagnetic separation acid treatment
    Glenn E Tillman
    US Department of Agriculture, Food Safety and Inspection Service, Eastern Laboratory Outbreaks Section, Athens, Georgia 30605, USA
    J Food Prot 75:1548-54. 2012
    ..In this study, an agar medium with lower supplement concentrations, modified Rainbow agar (mRBA), was evaluated for recovery of STEC ..
  25. ncbi Culture of Piscirickettsia salmonis on enriched blood agar
    Michael J Mauel
    Thad Cochran National Warmwater Aquaculture Center, Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, PO Box 197, Stoneville, MS 38776, USA
    J Vet Diagn Invest 20:213-4. 2008
    ..This report describes the successful culture of P. salmonis on enriched blood agar.
  26. ncbi Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenic agar: a prospective study to evaluate costs and effects
    M W M Wassenberg
    Department of Medical Microbiology Department of Internal Medicine and Infectious Diseases, University Medical Center, Utrecht, The Netherlands
    Clin Microbiol Infect 16:1754-61. 2010
    ..BD Diagnostics, San Diego, CA, USA), Xpert MRSA (`GeneXpert') (Cepheid, Sunnyvale, CA, USA) and chromogenic agar (MRSA-ID) (bioMérieux, Marcy-l'Etoile, France) was performed in 14 Dutch hospitals...
  27. ncbi Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method
    Boyan Bonev
    School of Biomedical Sciences and Institute of Infection and Immunity, University of Nottingham, Nottingham NG7 2UH, UK
    J Antimicrob Chemother 61:1295-301. 2008
    The agar diffusion assay is one method for quantifying the ability of antibiotics to inhibit bacterial growth...
  28. ncbi A novel agar medium to detect hydrogen peroxide-producing bacteria based on the prussian blue-forming reaction
    Mitsumasa Saito
    Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Maidashi, Fukuoka, Japan
    Microbiol Immunol 51:889-92. 2007
    The classic method for H(2)O(2) detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H(2)O(2) produced by bacteria...
  29. ncbi Thin agar layer method for recovery of heat-injured Listeria monocytogenes
    D H Kang
    Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506 1600, USA
    J Food Prot 62:1346-9. 1999
    A thin agar layer (TAL) method was developed to recover heat-injured Listeria monocytogenes. Modified Oxford medium (MOX), a selective plating medium, inhibits heat-injured L...
  30. pmc The speed of sound and attenuation of an IEC agar-based tissue-mimicking material for high frequency ultrasound applications
    Chao Sun
    Centre for Cardiovascular Science, The University of Edinburgh, Edinburgh, United Kingdom
    Ultrasound Med Biol 38:1262-70. 2012
    This study characterized the acoustic properties of an International Electromechanical Commission (IEC) agar-based tissue mimicking material (TMM) at ultrasound frequencies in the range 10-47 MHz...
  31. ncbi Agar degradation by microorganisms and agar-degrading enzymes
    Won Jae Chi
    Division of Bioscience and Bioinformatics, Myongji University, Yongin, Gyeonggi Do, Korea
    Appl Microbiol Biotechnol 94:917-30. 2012
    b>Agar is a mixture of heterogeneous galactans, mainly composed of 3,6-anhydro-L-galactoses (or L-galactose-6-sulfates) D-galactoses and L-galactoses (routinely in the forms of 3,6-anhydro-L-galactoses or L-galactose-6-sulfates) alternately ..
  32. pmc Analysis of keystone enzyme in Agar hydrolysis provides insight into the degradation (of a polysaccharide from) red seaweeds
    Jan Hendrik Hehemann
    Department of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, British Columbia V8W 3P6, Canada
    J Biol Chem 287:13985-95. 2012
    ..This latter feature differs from the vast majority of glycoside hydrolases, which use a carboxylic acid, highlighting the alternative strategies that enzymes may utilize in catalyzing the cleavage of glycosidic bonds...
  33. pmc Rapid isolation and susceptibility testing of Leptospira spp. using a new solid medium, LVW agar
    Vanaporn Wuthiekanun
    Mahidol Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand
    Antimicrob Agents Chemother 57:297-302. 2013
    ..These included the type of base agar, the concentration of rabbit serum (RS), and the concentration and duration of CO(2) incubation during the initial ..
  34. ncbi Application of thin agar layer method for recovery of injured Salmonella typhimurium
    D H Kang
    Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506 1600, USA
    Int J Food Microbiol 54:127-32. 2000
    ..a selective plating medium, can inhibit heat-injured Salmonella typhimurium from growing, whereas tryptic soy agar (TSA), a nonselective medium, does not. To facilitate recovery of heat-injured S...
  35. ncbi Use of an agar-gel technique for large scale application to recover Ascaris suum larvae from intestinal contents of pigs
    H C Slotved
    Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark
    Acta Vet Scand 38:207-12. 1997
    ..The contents were mixed with agar to a final concentration of 1% agar and allowed to sediment...
  36. pmc Differentiation of murine male germ cells to spermatozoa in a soft agar culture system
    Mahmoud Abu Elhija
    The Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Asian J Androl 14:285-93. 2012
    ..In the present study, we developed in vitro culture conditions using three-dimensional agar culture system (SACS), which has the capacity to induce testicular germ cells to reach the final stages of ..
  37. pmc A supplemented soft agar chemotaxis assay demonstrates the Helicobacter pylori chemotactic response to zinc and nickel
    Lisa Sanders
    Department of Microbiology and Environmental Toxicology, University of California at Santa Cruz, Santa Cruz, CA, USA
    Microbiology 159:46-57. 2013
    ..this conclusion, we employed both a temporal chemotaxis assay based on bacterial reversals and a supplemented soft agar spatial assay. We refined the temporal assay using a previously described chemorepellent, acid, and found that H...
  38. pmc Can the diagnosis of recurrent vulvovaginal candidosis be improved by use of vaginal lavage samples and cultures on chromogenic agar?
    N Novikova
    Department of Obstetrics and Gynecology, Lund University Hospital, SE 221 85 Lund, Sweden
    Infect Dis Obstet Gynecol 10:89-92. 2002
    To investigate if introital and vaginal flushing samples inoculated on chromogenic agar could increase the recovery rate and rapid identification of Candida and non-albicans species, as compared to culture of posterior vaginal fornix ..
  39. pmc Implementation of the thin layer agar method for diagnosis of smear-negative pulmonary tuberculosis in a setting with a high prevalence of human immunodeficiency virus infection in Homa Bay, Kenya
    Anandi Martin
    Institute of Tropical Medicine, Antwerp, Belgium
    J Clin Microbiol 47:2632-4. 2009
    The objective of this study was to evaluate the performance of a low-cost method, the thin layer agar (TLA) method, for the diagnosis of smear-negative patients. This prospective study was performed in Homa Bay District Hospital in Kenya...
  40. pmc Evaluation of five enzyme-linked immunosorbent assays and an agar gel immunodiffusion test for detection of antibodies to small ruminant lentiviruses
    J Brinkhof
    AHS Deventer, P O Box 9, 7400 AA Deventer, The Netherlands
    Clin Vaccine Immunol 14:1210-4. 2007
    ..To improve the quality of testing, five enzyme-linked immunosorbent assays (ELISAs) and an agar gel immunodiffusion test (AGIDT) were evaluated...
  41. ncbi Synthesis of agar microparticles using temperature-controlled microfluidic devices for Cordyceps militaris cultivation
    Yung Sheng Lin
    Department of Applied Cosmetology and Graduate Institute of Cosmetic Science, Hungkuang University, Taiwan
    Electrophoresis 32:3157-63. 2011
    A temperature-controlled microfluidic approach was developed for fabricating monodispersed agar beads with the potential to be a brand-new strategy for cultivating Cordyceps militaris...
  42. ncbi Inhibitory effects of silver ions on Legionella pneumophila grown on agar, intracellular in Acanthamoeba castellanii and in artificial biofilms
    C Unger
    Institute of Medical Microbiology and Hygiene, Faculty of Medicine Carl Gustav Carus, University of Technology, Dresden, Germany
    J Appl Microbiol 112:1212-9. 2012
    ..We undertook a series of experiments to investigate the susceptibility of Legionella pneumophila grown under extracellular and intracellular conditions and other water-related bacteria to silver ions...
  43. pmc Evaluation of Brilliance ESBL agar, a novel chromogenic medium for detection of extended-spectrum-beta- lactamase-producing Enterobacteriaceae
    Te Din Huang
    Laboratoire de Microbiologie, Cliniques Universitaires Mont Godinne UCL, 1 avenue G Therasse, 5530 Yvoir, Belgium
    J Clin Microbiol 48:2091-6. 2010
    The aim of this study was to evaluate the performance of Brilliance ESBL agar (OX; Oxoid, Basingstoke, United Kingdom), a novel chromogenic agar for the selective isolation and the presumptive identification of extended-spectrum-beta-..
  44. ncbi Simplified sunflower (Helianthus annuus) seed agar for differentiation of Candida dubliniensis from Candida albicans
    Z U Khan
    Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait
    Clin Microbiol Infect 10:590-2. 2004
    This study evaluated sunflower (Helianthus annuus) seed agar (SSA) for differentiation of Candida dubliniensis from Candida albicans on the basis of colony characteristics and chlamydospore production...
  45. ncbi Isolation of novel bacteria and actinomycetes using soil-extract agar medium
    Takefumi Hamaki
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399 4598, Japan
    J Biosci Bioeng 99:485-92. 2005
    ..discovered using an isolation technique consisting of (i) selection of microorganisms that grew on soil-extract agar medium, but not on conventional media, and (ii) detection of small microbial colonies with a microscope...
  46. pmc Evaluation of agar plates for direct enumeration of Campylobacter spp. from poultry carcass rinses
    Omar A Oyarzabal
    Department of Poultry Science, 260 Lem Morrison Drive, Auburn University, Auburn, Alabama 36849 5416, USA
    Appl Environ Microbiol 71:3351-4. 2005
    ..from poultry carcass rinses. Campy-Cefex and its modification produced the best results but were statistically similar to CAMPY, mCCDA, and Karmali...
  47. pmc Accurate, precise modeling of cell proliferation kinetics from time-lapse imaging and automated image analysis of agar yeast culture arrays
    Najaf A Shah
    Department of Genetics, University of Alabama School of Medicine, Birmingham, Alabama 35294, USA
    BMC Syst Biol 1:3. 2007
    ..cerevisiae gene deletion strains. Such studies have thus far been predominantly qualitative, using agar cell arrays to subjectively score growth differences...
  48. pmc Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test
    David P Kateete
    Department of Medical Microbiology, School of Biomedical Sciences, Makerere University College of Health Sciences, Upper Mulago hill road, Kampala, Uganda
    Ann Clin Microbiol Antimicrob 9:23. 2010
    ..aureus and is routinely done using either human or sheep plasma. This study evaluated Mannitol salt agar and the deoxyribonuclease (DNase) test for improving the efficiency of the tube coagulase test in resource limited ..
  49. pmc Comparison of Ashdown's medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei
    Sharon J Peacock
    Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
    J Clin Microbiol 43:5359-61. 2005
    Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens that proved positive for this organism...
  50. pmc Lateral flagella and swarming motility in Aeromonas species
    Sylvia M Kirov
    Discipline of Pathology, University of Tasmania, Hobart, Tasmania 7001, Australia
    J Bacteriol 184:547-55. 2002
    ..We established a reproducible swarming assay (0.5% Eiken agar in Difco broth, 30 degrees C) for Aeromonas spp...
  51. pmc Inhibition of PCR by agar from bacteriological transport media
    A P Gibb
    Provincial Laboratory for Public Health of Southern Alberta, Department of Microbiology and Infectious Diseases, University of Calgary, Canada
    J Clin Microbiol 36:275-6. 1998
    ..Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent...
  52. pmc Laboratory and clinical evaluation of screening agar plates for detection of carbapenem-resistant Enterobacteriaceae from surveillance rectal swabs
    Amos Adler
    Molecular Epidemiology and Antimicrobial Resistance Laboratory, Division of Epidemiology, Clinical Microbiology Laboratory, Tel Aviv Medical Center, Tel Aviv, Israel
    J Clin Microbiol 49:2239-42. 2011
    ..Three agar-based methods for direct CRE detection from rectal swabs were compared: CHROMagar-KPC (Chrom); MacConkey agar with ..
  53. ncbi Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA
    K B Anand
    Department of Microbiology, Armed Forces Medical College, Pune 440 040, India
    Indian J Med Microbiol 27:27-9. 2009
    ..The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecA gene by PCR.
  54. ncbi Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods
    Lyudmila Boyanova
    Department of Microbiology, Medical University of Sofia, Zdrave Street 2, 1431 Sofia, Bulgaria
    J Med Microbiol 54:481-3. 2005
    ..By the agar-well diffusion method, only 13...
  55. ncbi Improving the reproducibility of Pseudomonas aeruginosa swarming motility assays
    Julien Tremblay
    INRS Institut Armand Frappier, Laval Quebec, Canada
    J Basic Microbiol 48:509-15. 2008
    ..aeruginosa and show how different key parameters affect this type of motility (i.e. agar %, drying time under laminar flow, incubation temperature and pH)...
  56. pmc Phenotypic switching in Candida lusitaniae on copper sulfate indicator agar: association with amphotericin B resistance and filamentation
    Nancy S Miller
    Boston Medical Center, Department of Laboratory Medicine, 88 East Newton Street H3600, Boston, MA 02118, USA
    J Clin Microbiol 44:1536-9. 2006
    ..CFU of an AmB-resistant (MIC of 16 to 32 microg/ml) clinical strain was plated on yeast-peptone-dextrose (YPD) agar with 1 mM CuSO(4), three colony colors were observed: light brown (LB) >> dark brown (DB) > white (W), ..
  57. pmc CHROMagar Yersinia, a new chromogenic agar for screening of potentially pathogenic Yersinia enterocolitica isolates in stools
    Nicolas Renaud
    Lille University Medical Center, Lille, France
    J Clin Microbiol 51:1184-7. 2013
    ..hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) but was significantly more specific and had a very low false-positive rate...
  58. ncbi Natural occurrence and growth reaction on canavanine-glycine-bromothymol blue agar of non-neoformans Cryptococcus spp. in Malaysia
    S T Tay
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    Mycoses 51:515-9. 2008
    ..Upon testing on canavanine-glycine-bromothymol blue (CGB) agar, all the C. albidus isolates remained unchanged. Interestingly, a total of 29 (76.3%) C...
  59. ncbi Screening femoral heads from living donors: a prospective study comparing swab-agar versus bone fragment-broth culture
    Veroniek Saegeman
    Department of Medical Diagnostic Sciences, University Hospital, Leuven, Belgium
    Acta Orthop Belg 77:381-5. 2011
    ..transport medium taken intraoperatively, subsequently transported to the microbiology laboratory and inoculated on agar and in broth was compared with the recovery from a bone fragment also taken intraoperatively but immediately ..
  60. pmc Investigation of reduced susceptibility to glycopeptides among methicillin-resistant Staphylococcus aureus isolates from patients in Ireland and evaluation of agar screening methods for detection of heterogeneously glycopeptide-intermediate S. aureus
    Margaret M Fitzgibbon
    National MRSA Reference Laboratory, St James s Hospital, James s St, Dublin 8, Ireland
    J Clin Microbiol 45:3263-9. 2007
    Methicillin-resistant Staphylococcus aureus (MRSA) isolates (n = 3,189) from 2,990 patients were investigated by agar screening and by the Etest macromethod for reduced susceptibility to glycopeptide. No vancomycin-resistant S...
  61. ncbi Comparison of commercial enzyme-linked immunosorbent assay kits with agar gel precipitation and hemagglutination-inhibition tests for detecting antibodies to avian influenza viruses
    Rikiya Shiraishi
    Research Institute for Animal Science in Biochemistry and Toxicology, 3 7 11 Hashimotodai, Midori ku, Sagamihara, Kanagawa 252 0132, Japan
    J Vet Med Sci 74:1217-20. 2012
    ..The sensitivities and specificities of the ELISA kits were compared with those of the agar gel precipitation (AGP) and hemagglutination-inhibition (HI) tests...
  62. ncbi Comparison of culture screening protocols for methicillin-resistant Staphylococcus aureus (MRSA) using a chromogenic agar (MRSA-Select)
    Seungok Lee
    Department of Laboratory Medicine, College of Medicine, Catholic University of Korea, Holy Family Hospital, Pucheon, Korea
    Ann Clin Lab Sci 38:254-7. 2008
    ..One swab was plated directly on MRSA-Select agar (D-MRSA-Select) and observed at 24 hr. The duplicate swab was incubated in tryptic soy broth (TSB) with 6...
  63. pmc Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens
    Bo Moon Shin
    Department of Laboratory Medicine, Inje University, Sanggye Paik Hospital, Seoul, Korea
    Ann Lab Med 34:15-9. 2014
    ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile...
  64. ncbi Improved recovery of Clostridium difficile spores with the incorporation of synthetic taurocholate in cycloserine-cefoxitin-fructose agar (CCFA)
    Niki Francis Foster
    School of Pathology and Laboratory Medicine, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia
    Pathology 44:354-6. 2012
    ..The aim of this study was to quantify the effect of the known germinant sodium taurocholate on recovery of C. difficile spores and to determine if the supplement impacts on the recovery of vegetative C. difficile...
  65. ncbi Determination of mercury in water and fish samples by cold vapor atomic absorption spectrometry after solid phase extraction on agar modified with 2-mercaptobenzimidazole
    N Pourreza
    Department of Chemistry, College of Science, Shahid Chamran University, Ahvaz, Iran
    J Hazard Mater 161:982-7. 2009
    ..The Hg(II) ions were retained on a mini-column packed with agar powder modified with 2-mercaptobenzimidazole at a flow rate of 6 mL min(-1)...
  66. ncbi Real-time evaluation of an optimized real-time PCR assay versus Brilliance chromogenic MRSA agar for the detection of meticillin-resistant Staphylococcus aureus from clinical specimens
    J Danial
    Department of Microbiology, Royal Infirmary of Edinburgh, Edinburgh, UK
    J Med Microbiol 60:323-8. 2011
    ..This study showed that implementation of a molecular test can be achieved with limited resources in a standard microbiology laboratory...
  67. ncbi Comparative evaluation of a chromogenic agar medium-PCR protocol with a conventional method for isolation of Vibrio parahaemolyticus strains from environmental and clinical samples
    Adrian Canizalez-Roman
    School of Medicine, Autonomous University of Sinaloa, 80246 Culiacán Sinaloa, Mexico
    Can J Microbiol 57:136-42. 2011
    ..Vibrio (CV) medium-PCR protocol and the conventional method (TCBS (thiosulfate-citrate-bile salts-sucrose) agar plus biochemical and Wagatsuma agar tests) for detection of V...
  68. ncbi Diversity of Pseudomonas strains isolated with King's B and Gould's S1 agar determined by repetitive extragenic palindromic-polymerase chain reaction, 16S rDNA sequencing and Fourier transform infrared spectroscopy characterisation
    K Johnsen
    Department of Ecology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    FEMS Microbiol Lett 173:155-62. 1999
    King's B and Gould's S1 agar were compared with regard to the isolation of Pseudomonas from four environmental samples...
  69. ncbi Thin layer agar compared to BACTEC MGIT 960 for early detection of Mycobacterium tuberculosis
    Anandi Martin
    Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium
    J Microbiol Methods 78:107-8. 2009
    We compared the sensitivity and time to detection of growth of Mycobacterium tuberculosis in the thin layer agar (TLA) compared to BACTEC MGIT960. The average time for growth of M. tuberculosis in TLA and BACTEC MGIT960 was 10.6 and 9...
  70. ncbi In vitro susceptibility of Bartonella species to 17 antimicrobial compounds: comparison of Etest and agar dilution
    Christina Dörbecker
    Institute for Medical Microbiology and Hygiene, University of Freiburg, Germany
    J Antimicrob Chemother 58:784-8. 2006
    ..strains including 21 Bartonella henselae isolates was performed for 17 antimicrobial agents (telithromycin, four macrolides, five fluoroquinolones, five aminoglycosides, doxycycline and rifampicin)...
  71. ncbi Specificity of absorbed ELISA and agar gel immuno-diffusion tests for paratuberculosis in goats with observations about use of these tests in infected goats
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570
    Aust Vet J 81:71-5. 2003
    ..To determine the specificity of serological tests that are currently used in veterinary diagnostic laboratories in Australia for detection of Mycobacterium avium subsp paratuberculosis infection in goats...
  72. ncbi Obstructing small bowel bezoars due to an agar diet: diagnosis using double balloon enteroscopy
    Taro Osada
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Intern Med 47:617-20. 2008
    ..The patient reported that she had eaten a large amount of highly-concentrated, agar dissolved in boiling water two days prior to presentation...
  73. ncbi Xanthan gum: an economical substitute for agar in plant tissue culture media
    R Jain
    Department of Botany, University of Delhi, Delhi 7, India
    Plant Cell Rep 25:81-4. 2006
    ..Its suitability as a substitute to agar was demonstrated for in vitro seed germination, caulogenesis and rhizogenesis of Albizzia lebbeck, androgenesis in ..
  74. ncbi Validation of the agar proportion and 2 liquid systems for testing the susceptibility of Mycobacterium tuberculosis to moxifloxacin
    Claudio Piersimoni
    Department of Clinical Microbiology, United Hospitals, 60020 Ancona, Italy
    Diagn Microbiol Infect Dis 57:283-7. 2007
    ..sought to set up susceptibility test conditions for Mycobacterium tuberculosis against MOX by using the reference agar proportion method, the BACTEC 460 radiometric system, and the recently validated nonradiometric BACTEC MGIT 960 ..
  75. pmc Evaluation of a novel chromogenic agar medium for isolation and differentiation of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis isolates
    Nathan A Ledeboer
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Clin Microbiol 45:1556-60. 2007
    ..To this end, we evaluated a prototype chromogenic agar medium (VRE-BMX; bioMérieux, Marcy l'Etoile, France) used to recover VRE from clinical specimens...
  76. pmc Susceptibilities of Mycobacterium tuberculosis to isoniazid and rifampin on blood agar
    Ahmet Yilmaz Coban
    Ondokuz Mayis University, Medical School, Department of Microbiology and Clinical Microbiology, 55139 Samsun, Turkey
    J Clin Microbiol 43:1930-1. 2005
    In this study, blood agar was used instead of 7H10 agar for the susceptibility testing of 34 clinical isolates of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) in accordance with the NCCLS...
  77. pmc Tobacco agar, a new medium for differentiating Candida dubliniensis from Candida albicans
    Zia U Khan
    Department of Microbiology, Faculty of Medicine, Kuwait University, Safat, Kuwait
    J Clin Microbiol 42:4796-8. 2004
    ..In this study, we have evaluated the efficacy of tobacco agar for the differentiation of C. dubliniensis from C. albicans. On this medium at 28 degrees C, all 30 C...
  78. ncbi Self-produced extracellular stimuli modulate the Pseudomonas aeruginosa swarming motility behaviour
    Julien Tremblay
    INRS Institut Armand Frappier, Laval Quebec, H7V 1B7, Canada
    Environ Microbiol 9:2622-30. 2007
    ..iii) di-RLs and HAAs display different diffusion kinetics on a surface as di-RLs spread faster than HAAs in agar, (iv) di-RLs and HAAs have no effect on swimming cells, suggesting that swarming cells are different from swimming ..
  79. ncbi Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium
    Lyudmila A Romanenko
    Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospekt 100 Let Vladivostoku, 159 Russia
    Int J Syst Evol Microbiol 53:647-51. 2003
    ..in their lack of pigment production, their minimal and maximal growth temperatures and their ability to hydrolyse agar and carrageenan and in the utilization of organic compounds...
  80. pmc Testing susceptibility of multidrug-resistant Mycobacterium tuberculosis to second-line drugs by use of blood agar
    Dilek Satana
    Istanbul University, Istanbul Medical School, Department of Microbiology and Clinical Microbiology, Istanbul, Turkey
    J Clin Microbiol 48:4291-3. 2010
    ..acid (PAS), capreomycin (CAP), clofazimine (CFM), and ethionamide (ETH), were investigated on blood agar according to CLSI recommendations...
  81. ncbi Effect of ionic strength on surface-selective patch binding-induced phase separation and coacervation in similarly charged gelatin-agar molecular systems
    Shilpi Boral
    Polymer and Biophysics Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
    J Phys Chem B 114:12027-35. 2010
    ..of surface patch binding-induced protein-polysaccharide interaction leading to complex coacervation, between agar (a polyanionic polysaccharide) and gelatin B (a polyampholyte protein), both having similar net charge, at a ..
  82. pmc Agar-based disk diffusion assay for susceptibility testing of dermatophytes
    E I Nweze
    Center for Medical Mycology, University Hospitals Case Medical Center and Case Western Reserve University, Cleveland, OH 44106, USA
    J Clin Microbiol 48:3750-2. 2010
    Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes...
  83. ncbi A comparison of thiosulphate-citrate-bile salts-sucrose (TCBS) agar and thiosulphate-chloride-iodide (TCI) agar for the isolation of Vibrio species from estuarine environments
    C Pfeffer
    Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, USA
    Lett Appl Microbiol 36:150-1. 2003
    ..The purpose of this study was to compare a recently described medium, thiosulphate-chloride-iodide (TCI), for the isolation of estuarine vibrios with thiosulphate-citrate-bile salts-sucrose (TCBS)...
  84. pmc Detection of intermediately vancomycin-susceptible and heterogeneous Staphylococcus aureus isolates: comparison of Etest and Agar screening methods
    K Riederer
    Department of Medicine, St John Hospital and Medical Center, Detroit, Michigan, USA
    J Clin Microbiol 49:2147-50. 2011
    ..Growth on BHI-V4 was noted in all VISA and four (12.1%) hVISA isolates. None of the S-MRSA isolates grew on BHI-V4 agar. The sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values were 75.0%, 89.0%, 38...
  85. ncbi [Evaluation of blood agar medium for the growth of mycobacteria]
    Ahmet Yilmaz Coban
    Ondokuz Mayis University Faculty of Medicine, Department of Medical Microbiology, Samsun, Turkey
    Mikrobiyol Bul 45:617-22. 2011
    This study was aimed to evaluate the performance of blood agar for the growth of mycobacteria from clinical specimens sent to Mycobacteriology Laboratory of Samsun Chest Diseases Hospital...
  86. ncbi Fusobacterium necrophorum- detection and identification on a selective agar
    Steffen Bank
    Department of Clinical Microbiology, Viborg Hospital, Denmark
    APMIS 118:994-9. 2010
    ..In our laboratory, a F. necrophorum selective agar has been developed containing vancomycin and nalidixin, which inhibit the growth of most Gram-positive and many ..
  87. ncbi Agar polysaccharides from Gracilaria species (Rhodophyta, Gracilariaceae)
    E Marinho-Soriano
    Departamento de Oceanografia e Limnologia, Laboratorio de Macroalgas, Universidade Federal do Rio Grande do Norte, Via Costeira, Praia de Mãe Luiza s n, 59014 100, NatalRN, Brazil
    J Biotechnol 89:81-4. 2001
    Yield, physical and chemical properties of agar from three agarophytes species (Gracilaria gracilis, G. dura and G. bursa-pastoris) were determined. The agar yield from the three species varied significantly (P<0.01)...
  88. ncbi Comparative evaluation of chromogenic agar CM1046 and mFC agar for detection of E. coli and thermotolerant coliform bacteria from water samples
    T D Wohlsen
    Unitywater, Maroochydore Sewage Treatment Plant, Maroochydore, Queensland, Australia
    Lett Appl Microbiol 53:155-60. 2011
    The equivalence of Oxoid (CM 1046) Brilliance((TM)) E. coli/coliform selective agar to mFC agar, as used in the Australian/New Zealand Standard Method to detect thermotolerant coliforms and Escherichia coli in water samples, was assessed.
  89. ncbi Biostimulation of estuarine microbiota on substrate coated agar slides: a novel approach to study diversity of autochthonous Bdellovibrio- and like organisms
    Ashvini Chauhan
    Marine Molecular Microbial Ecology Laboratory, Environmental Sciences Institute, Florida A and M University, 1515, S MLK Blvd, 305 FSHSRC, Tallahassee, FL 32307, USA
    Microb Ecol 55:640-50. 2008
    ..b>Agar spiked with biostimulants such as yeast extract (YE), casamino acids (CA), or concentrated cells of Vibrio ..
  90. ncbi Stability of agar in the seaweed Gracilaria eucheumatoides (Gracilariales, Rhodophyta) during postharvest storage
    Jumelita B Romero
    Mindanao State University Tawi Tawi, Sanga Sanga, Bongao, 7500 Tawi Tawi, Philippines
    Bioresour Technol 99:8151-5. 2008
    ..The yield, chemical composition, physical and textural properties of alkali-treated agar extract was determined at different time intervals within 31 months of storage at dried state after harvest...
  91. ncbi The acoustic properties, centered on 20 MHZ, of an IEC agar-based tissue-mimicking material and its temperature, frequency and age dependence
    M P Brewin
    Department of Clinical Physics, Royal London Hospital, London, UK
    Ultrasound Med Biol 34:1292-306. 2008
    The purpose of this study was to characterize the ultrasonic properties of agar-based tissue-mimicking materials (TMMs) at ultrasound frequencies centered around 20 MHz...
  92. ncbi A clinical evaluation of the agar alginate combined impression: dimensional accuracy of dies by new master crown technique
    Priyadarshanee Manorika Ratnaweera
    Fixed Prosthodontics, Department of Restorative Sciences, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Japan
    J Med Dent Sci 50:231-8. 2003
    To evaluate the dimensional accuracy of several impression methods including agar alginate combined impression in vivo; the marginal accuracy of stone dies was determined using a new electroformed master crown technique.
  93. ncbi Agar-plated bacteria found in the activated sludge of lab-scale SBR and CFSTR systems
    D F Juang
    Department of Healthcare Administration, Mei Ho Institute of Technology, 23, Ping Kuang Road, Nei Pu, Ping Tong 912, Taiwan, ROC
    Bioresour Technol 89:75-9. 2003
    We identified and compared the predominant agar-plated bacteria in the activated sludge of two popular wastewater treatment systems, the sequencing batch reactor (SBR) and the continuous-flow stirred tank reactor (CFSTR)...
  94. ncbi Antibacterial properties of aged dental cements evaluated by direct-contact and agar diffusion tests
    Israel Lewinstein
    Department of Oral Rehabilitation, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
    J Prosthet Dent 93:364-71. 2005
    ..Since failure of fixed partial dentures is most frequently caused by caries, it would be advantageous if cements possessed antibacterial properties...
  95. ncbi Evaluation of immunomagnetic separation in combination with ALOA Listeria chromogenic agar for the isolation and identification of Listeria monocytogenes in ready-to-eat foods
    Shaila Wadud
    Laboratory Services Division, University of Guelph, 95 stone Rd West, Guelph, ON, Canada N1H 8J7
    J Microbiol Methods 81:153-9. 2010
    ..IMS-ALOA method is a valuable alternate test method for the screening of L. monocytogenes in a variety of foods especially ready-to-eat foods...
  96. ncbi Evaluation of blood agar for susceptibility testing of Mycobacterium tuberculosis against first-line antituberculous drugs: results from two centers
    A Y Coban
    J Chemother 20:388-90. 2008
  97. pmc Validation of the use of Middlebrook 7H10 agar, BACTEC MGIT 960, and BACTEC 460 12B media for testing the susceptibility of Mycobacterium tuberculosis to levofloxacin
    Cynthia A Sanders
    California Department of Health Services, Richmond, CA 94804, USA
    J Clin Microbiol 42:5225-8. 2004
    ..test conditions for Mycobacterium tuberculosis against levofloxacin by the traditional reference method, agar proportion (AP), the commonly used BACTEC 460 radiometric system, and the newer BACTEC MGIT 960 method...
  98. pmc Factors affecting daughter cells' arrangement during the early bacterial divisions
    Pin Tzu Su
    Institute of Microbiology and Immunology, National Yang Ming University, Taipei, Taiwan, Republic of China
    PLoS ONE 5:e9147. 2010
    On agar plates, daughter cells of Escherichia coli mutually slide and align side-by-side in parallel during the first round of binary fission...
  99. ncbi Antimicrobial activity of highly stable silver nanoparticles embedded in agar-agar matrix as a thin film
    S Ghosh
    Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560 012, India
    Carbohydr Res 345:2220-7. 2010
    Highly stable silver nanoparticles (Ag NPs) in agar-agar (Ag/agar) as inorganic-organic hybrid were obtained as free-standing film by in situ reduction of silver nitrate by ethanol...
  100. pmc Differentiation of Candida dubliniensis from Candida albicans on staib agar and caffeic acid-ferric citrate agar
    A Al Mosaid
    Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland
    J Clin Microbiol 39:323-7. 2001
    ..dubliniensis to produce rough colonies and chlamydospores (chlamydoconidia) on Staib agar (SA) provided a simple means of differentiating it from its close relative C. albicans...
  101. ncbi Comparison of microbial adherence to antiseptic and antibiotic central venous catheters using a novel agar subcutaneous infection model
    Trupti A Gaonkar
    College of Physicians and Surgeons, Department of Surgery, 630 West 168th Street, New York, NY 10032, USA
    J Antimicrob Chemother 52:389-96. 2003
    An agar subcutaneous infection model (agar model), which simulates the rat subcutaneous infection model (rat model), was developed to assess the ability of antimicrobial catheters to resist microbial colonization...

Research Grants63

  1. THE ROLE OF CELL SHAPE AND CELL WALL IN HELICOBACTER PYLORI PATHOGENESIS
    NINA REDA SALAMA; Fiscal Year: 2010
    ..All mutants tested show slight, but reproducible defects in halo formation in soft agar suggesting compromised swimming or chemotaxis...
  2. Role of IGF-IR tyrosine kinase in NPM-ALK-expressing T-cell lymphoma
    Hesham M Amin; Fiscal Year: 2013
    ..An experimental inhibitor of IGF-IR induced apoptosis and cell cycle arrest and abrogated colony formation in soft agar of NPM-ALK+ ALCL cell lines...
  3. EGFRvIII in Pancreatic Cancer
    Maneesh Jain; Fiscal Year: 2010
    ..cells is sufficient to lead to a transformed phenotype;that the cells will gain the ability to form foci in soft agar and to form tumors in nude mice...
  4. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2013
    ..Additionally, we discovered that Lp exhibits a swarming phenotype on agar surfaces that is dependent upon T2S...
  5. Inflammasomes in pathogenesis of mesothelioma
    Arti Shukla; Fiscal Year: 2013
    ..markers of mesothelial to fibroblast transition (MFT) and colony formation ability of these cells in soft agar. Also, the IL-1[unreadable] receptor blocker Anakinra, will also be used to confirm the role of IL-1 in these ..
  6. Ack1: Activation and Consequences in Prostate Cancer
    HENRY SHELTON EARP; Fiscal Year: 2012
    ..control or kinase dead Ack1), demonstrated minimally increased growth in culture, substantially increased soft agar growth and, as noted, remarkably accelerated tumorigenesis in nude mice, even when castrated prior to tumor ..
  7. Wnt antagonist genes in kidney tumor progression and metastasis
    Rajvir Dahiya; Fiscal Year: 2012
    ..Also analyze in vitro invasiveness (extra-cellular matrix binding assay, invasion assay and soft agar colony forming efficiency) of transfected and parental cells...
  8. A Novel Surface Sensing Pathway in Proteus mirabilis
    Philip N Rather; Fiscal Year: 2013
    ..When grown on solid surfaces, such as Foley catheters or agar plates, P...
  9. Non-invasive Fluorescent Imaging Mycobacterium tuberculosis Extrapulmonary Infect
    Ying Kong; Fiscal Year: 2013
    ..Research on EPTB using animal models is hampered by slow growth of M. tuberculosis on agar plates...
  10. Dissecting the role of mitochondrial DNA mutations in cancer
    Sean David Taylor; Fiscal Year: 2013
    ..Tumorigenic potential will be measured using soft agar and matrigel mobility assays...
  11. Mechanisms of Overexpressed TrkB in Inducing Pancreatic Cancer Metastasis
    Paul J Chiao; Fiscal Year: 2013
    ..Overexpression of TrkBT1 in nonmetastatic Colo357FG cells induced colony formation in soft agar and liver metastasis in nude mice, but how the TrkBT1 variant, which lacks kinase-domain, induces pancreatic cancer ..
  12. Hormone-regulated Prostate Cancer Metastasis
    Michael L Lu; Fiscal Year: 2010
    ..Ectopic expression of a PAK6 constitutive active mutant promotes cell motility and anchorage-independent soft agar cell growth of human prostate cancer LNCap cells...
  13. A Tumor Suppressor Function for the c-Fes Tyrosine Kinase in Colon Cancer
    THOMAS EDWARD SMITHGALL; Fiscal Year: 2012
    ..examined, and re-introduction of Fes using a recombinant retrovirus suppressed colony-forming activity in soft agar and invasiveness in the Matrigel assay...
  14. Identification and therapeutic application of miRNA-drivers in lung cancer
    ANDREA LYNN KASINSKI; Fiscal Year: 2013
    ..Kasinski is actively pursuing first in cell culture and will advance into animal models. This assay in soft agar is selecting for miRNAs that can specifically cause normal human lung bronchial epithelial cells to become ..
  15. Mechanism of Merlin-mediated contact inhibition and tumor suppression.
    Filippo G Giancotti; Fiscal Year: 2013
    ..Finally, depletion of DCAF1 suppresses the ability of Merlin-deficient tumor cells to grow in soft agar and to form tumors in nude mice...
  16. HDAC inhibition for the chemoprevention of lung cancer
    Johann Christoph Brandes; Fiscal Year: 2013
    ..the histone H3K9 methyltransferase G9A, together with a reduction in anchorage independent colony formation in soft-agar. We hypothesize that due to its direct and indirect epigenetic effects valproate can a) permanently reverse and b) ..
  17. Isolating novel fungi for antibiotic discovery
    Losee Lucy Ling; Fiscal Year: 2012
    ..The trap consists of two layers of sterile agar sandwiched between semi-permeable membranes that is inserted into soil...
  18. Cancer gene discovery using Sleeping Beauty transposon-mediated mutagenesis
    TIMOTHY KAEHLER STARR; Fiscal Year: 2010
    ..both normal and transformed, to determine their effect on growth, apoptosis, the ability to form colonies in soft agar, and regulation of key signaling pathways...
  19. SIGNAL TRANSDUCTION BY TYROSINE PHOSPHORYLATION
    TONY R HUNTER; Fiscal Year: 2013
    ..cell cycle progression, apoptosis and autophagy, morphological transformation, anchorage independent growth in soft agar, and tumorigenesis in nude mice...
  20. Searching for Novel Genes in Heparan Sulfate Biosynthesis
    Emylie Seamen; Fiscal Year: 2013
    ..Additionally, we will determine the ability of tumor cells expressing the candidate shRNAs to form colonies in soft agar and induce the formation of tumors in animals...
  21. Cancer gene discovery using Sleeping Beauty transposon-mediated mutagenesis
    TIMOTHY KAEHLER STARR; Fiscal Year: 2013
    ..both normal and transformed, to determine their effect on growth, apoptosis, the ability to form colonies in soft agar, and regulation of key signaling pathways...
  22. Development of a transformative approach to the rapid detection, isolation, and i
    BIRGIT URSULA JAKI; Fiscal Year: 2013
    ..TLC, automated "spray-on" sampling, gradient elution, semi-automatic extraction), microbiology (TB agar-overlay with luminescence detection), and MS spectroscopy (LC-MS2) will be used to target the isolation of lead ..
  23. Analysis of cap-dependent translational repression by microRNAs in oncogensis
    Carl D Novina; Fiscal Year: 2013
    ..and test proliferation rates, cell-cycle progression, escape from replicative senescence, colony formation in soft agar, and invasive activity...
  24. MicroRNA-190 and Oxidative Stress in Arsenic carcinogenesis
    Fei Chen; Fiscal Year: 2013
    ..basal or As3+-induced cell transformation and carcinogenesis by assays of anchorage-independent growth in soft agar (colony formation) and inoculation of the cells in the lung of nude mice...
  25. Monoclonal Antibody to FGF2 for Treatment of Hepatocellular Carcinoma and Other C
    K Jin Kim; Fiscal Year: 2010
    ..The ability of GAL-F2 to inhibit FGF2- induced cell proliferation and migration, and to inhibit soft agar colony formation by human hepatoma cell lines, will be investigated...
  26. A High-Throughput Screen for Specific Anti-M. tuberculosis Compounds
    Kim Lewis; Fiscal Year: 2012
    ..An environmental sample such as soil is mixed with agar and sandwiched between two semi-permeable membranes of a diffusion chamber which is returned to the environment...
  27. Proteus mirabilis swarming: Initiation and role during urinary tract infection
    CHELSIE ELIZABETH ARMBRUSTER; Fiscal Year: 2013
    ..because it will provide insight into how bacteria decide to perform coordinated multicellular functions, both on an agar surface and during infection of a host...
  28. Mechanics of Bacterial Swarming
    Howard C Berg; Fiscal Year: 2011
    ..and extend these measurements to the body of the swarm, and 3) to learn whether fluid is drawn from the underlying agar by osmotic flow...
  29. Silencing CD44v6 Expression Prevents Intestinal Tumor Growth
    Suniti Misra; Fiscal Year: 2013
    ..4) Knocking down CD44v6 in sorted cancer stem cells reverses their drug resistance and the growth in soft agar. 5) Knocking down CD44v6 in pre-adenoma Apc10...
  30. Role of Nuclear Factor I A (NFIA) gene in glioma
    Hae Ri Song; Fiscal Year: 2013
    ..experimental systems, however, overexpression of NFIA increased colony formation of U87 human GBM cell line in soft agar and accelerated growth of U87 intracranial tumors in mouse brains...
  31. The role of histone phosphorylation in arsenic-induced cell transformation and sk
    Zigang Dong; Fiscal Year: 2012
    ..histone H3 and H2B siRNA gene knockdown and overexpressing stable cell lines to test the role of H3 and H2B in soft agar cell transformation assays...
  32. Elucidating p53 transcriptional networks involved in pancreatic cancer suppressio
    Laura D Attardi; Fiscal Year: 2013
    ..We will identify those with tumor suppressor activity using RNA interference approaches in soft agar assays and orthotopic pancreatic cancer models...
  33. Susceptibility Testing, Treatment Choices, and Outcomes of MDR-GNB Infections
    Lisa Maragakis; Fiscal Year: 2009
    ..different in vitro microbiology testing methods such as automated susceptibility testing, E-test, disk diffusion, agar dilution, or various synergy tests lead to different treatment choices, including altered dosing or route of ..
  34. Mechanisms of Life Span Extension By Dietary Deprivation
    Matt Kaeberlein; Fiscal Year: 2012
    ..We have recently developed a novel DR method in C. elegans where adult animals are maintained on a nutrient-agar surface without bacterial food source (dietary deprivation = DD)...
  35. The role of entosis in human cancers
    MICHAEL H OVERHOLTZER; Fiscal Year: 2013
    ..of cells from extracellular matrix in vitro, and is prevalent in anchorage-independent growth assays in soft agar. In breast tumors, cell-in- cell structures are found in early-stage (DCIS) tumors, and also in late stage invasive ..
  36. ANTIBIOTIC SUSCEPTIBILITIES OF PERIODONTAL BACTERIA
    Clay Walker; Fiscal Year: 1993
    ..Minimal inhibitory concentrations (MICs) will be determined by agar dilution technique for these bacteria to determine if significant differences exist within species present both ..
  37. Evaluating Antimicrobial Compounds from Coral-Associated Pseudovibrio Bacteria
    Maria I Vizcaino; Fiscal Year: 2010
    ..We will use an agar- overlay antimicrobial assay to determine the antimicrobial production of P...
  38. PKCz;a novel therapeutic target for pancreatic cancer 'stem cells'
    Amanda M Butler; Fiscal Year: 2013
    ..the ability of these cells to clonaly expand, migrate toward the chemokine, SDF-1, enhanced ability to grow in soft agar, and increased expression of SC markers. Preliminary data suggests a role for PKC? in PCSCs...
  39. CLOSTRIDIUM DIFFICILE INFECTION
    JERGANG CHANG; Fiscal Year: 1991
    ..For specific aim 2, a comparison of 3 detection methods is proposed. The routine microbiological isolation on CCFA agar, detection of toxin A by EIA and detection of toxin B by cytotoxicity...
  40. Contact sensing and C. albicans-host interaction
    Carol A Kumamoto; Fiscal Year: 2013
    ..A plasma membrane protein that is needed for invasive growth in response to contact with agar medium and may be a sensor of contact has been characterized...
  41. Cellular Cooperation and Cheating: an Experimental and Mathematical Analysis
    Wenying Shou; Fiscal Year: 2009
    ..cheater tolerance of the cooperative system in a well-mixed liquid culture (no spatial structure) with those on an agar pad (with spatial structure)...
  42. Contact sensing and C. albicans-host interaction
    Carol Kumamoto; Fiscal Year: 2009
    ..A plasma membrane protein that is needed for invasive growth in response to contact with agar medium and may be a sensor of contact has been characterized...
  43. Functional analysis of transcriptional program regulated by the c-MYC oncoprotein
    Steven B McMahon; Fiscal Year: 2012
    ..mediated depletion of one of the novel targets, MTA1, blocks the ability of MYC to induce growth of cells in soft agar. Additional targets from the screen (POLRMT and CD30), have been thoroughly characterized at the molecular level...
  44. EFFECTS OF BENZO[A]PYRENE IN TRANSGENIC MODEL OF CERVICA
    ELLEN RORKE; Fiscal Year: 2002
    ..cervix compared to normal cervix, and examine the tumorigenic phenotype of the cells in nude mice, in soft agar, and in microinvasion assays...
  45. CTGF in Pancreatic Tumor Growth
    Amato J Giaccia; Fiscal Year: 2010
    ..Induction of CTGF had no effect on cell growth on plastic, but significantly enhanced spheroid growth in soft agar. Most striking was the increase in cell adhesion induced by CTGF expression...
  46. Mechanism of HTLV-1 activation of the SRF pathway.
    Maureen Shuh; Fiscal Year: 2006
    ..studies have shown that Tax induces the immortalization of primary cells and causes the formation of soft agar colonies and tumors in nude mice...
  47. Probes for fluids in bacterial swarms
    Howard C Berg; Fiscal Year: 2013
    ..g., agar. The cells become multinucleate, elongate, synthesize large numbers of flagella, and advance across the surface in ..
  48. ODC AS A THERAPEUTIC TARGET--GENE FUNCTION ANALYSIS
    Gordon Luk; Fiscal Year: 1992
    ..These parameters will be correlated with the cells' growth rate, ability to grow in soft agar, loss of contact inhibition, tumorigenic potential in nude mice, and response to ODC inhibition by difluoromethyl ..
  49. MOLECULAR BASIS FOR INVASIVENESS IN A PATHOGENIC FUNGUS
    ANNE DRANGINIS; Fiscal Year: 1999
    ..Diploid strains of Saccharomyces switch from a single cell form to a filamentous form, while haploid strains invade agar. Both these processes are regulated by a MAP kinase cascade in Saccharomyces that results in activation of the ..
  50. MECHANISM UNDERLYING DCYVD MEDIATED PREFERENTIAL STIMULA
    Kapil Bhalla; Fiscal Year: 1993
    ..In biologic studies, utilizing soft agar cloning methods, we will identify high dose dCyd and dCyd analog (e.g...
  51. GROWTH FACTOR SIGNALING AND TRAFFIC IN ANGIOGENESIS
    Igor Prudovsky; Fiscal Year: 2005
    ..soluble Jagged1 NIH 3T3 cells transfectants as assessed by low cell seed density-mediated colony formation in soft agar and a significant increase in the tyrosine phosphorylation of Src and it F-actin-binding protein substrate, ..
  52. ROLE OF PHOSPHOLIPASE C IN TRANSFORMATION
    Albert Wong; Fiscal Year: 1993
    ..and the properties associated with transformation including effects on cell growth, cloning efficiency in soft agar, and tumorigenicity in athymic mice...
  53. Azoles and Candida in AIDS - A Whole Cell Response
    THEODORE WHITE; Fiscal Year: 2004
    ..phenotype identified in our laboratory, in which certain susceptible isolates are able to form small colonies on agar plates containing fluconazole...
  54. Targeting HMGA1 in Pancreatic Tumor Progression
    Linda M S Resar; Fiscal Year: 2010
    ..Specifically, cells engineered to overexpress activated RAS and HMGA1a form foci in soft agar and tumors in nude mice...
  55. PHYSIOLOGY OF PITUITARY CELL GLUCOCORTICOID BINDING
    Robert Harrison; Fiscal Year: 1993
    ..cells were treated with a chemical mutagen and glucocorticoid-resistant clones identified as large colonies on soft agar containing dexamethasone + G418. Fourteen clones were studied...
  56. ANALYSIS OF IDIOTYPE MUTANTS
    Heinz Kohler; Fiscal Year: 1980
    ..We have cloned in soft agar several sublines from an in vitro adapted S107 line by overlay with heterologous anti-S107 sera and PC-antigen...
  57. Role for DKC1 in cell proliferation and transformation
    Faizan Alawi; Fiscal Year: 2009
    ..Rat 1a-MYC-ER fibroblasts;and investigate the ability of MYC-ER to stimulate anchorage-independent growth in soft agar. In Aim 2, we will investigate the effects of DKC1 overexpression in c-Myc-null and wild type Rat 1a cells...
  58. RANDOMIZED CLINICAL TRIAL OF A CAPILLARY CLONING SYSTEM
    Daniel Von Hoff; Fiscal Year: 1990
    ..However, there are considerable problems with the conventional two layer agar system performed in 35 mm petri dishes...
  59. TAXIS OF RHIZOBIA TO LOCALIZED SITES ON LEGUME ROOTS
    KOSTIA BERGMAN; Fiscal Year: 1990
    ..Attractant activity of purified chemicals and plant root extracts is assayed in a simple, sort agar plate assay...
  60. SWITCHING IN CANDIDA ALBICANS
    David Soll; Fiscal Year: 1990
    ..and at high frequency 10-2 between a number of specific general phenotypes identified by colony morphology on agar. These phenotypes include "star", "ring", "irregular wrinkle", "hat", "stipple" and "fuzzy"...
  61. Role of EGFR ligand-containing exosomes in colorectal cancer
    Robert J Coffey; Fiscal Year: 2013
    ..We will assess the contribution of exosomal AREG and mutant KRAS from mutant KRAS-expressing donor cells to soft agar growth, invasion and prolonged AKT signaling for recipient non-transformed intestinal epithelial cell lines...
  62. RAS MEDIATED, ANCHORAGE INDEPENDENT GROWTH
    ROBERT KRAUSS; Fiscal Year: 2002
    ..In contrast to their transformed parent line, these revertant cell lines do not form colonies in soft agar. Additionally, they suppress the transformed phenotype in somatic cell hybrids and are resistant to ..
  63. IkB Kinase alpha (IKKalpha): Role in Skin Carcinogenesis
    Yinling Hu; Fiscal Year: 2007
    ..Our preliminary results show that the immortalized IKKalpha-/- keratinocytes are able to form cell colonies in soft agar and grow into poorly differentiated spindle cell carcinomas in nude mice following subcutaneous injection...