polysaccharides

Summary

Top Publications

  1. ncbi Fucoidan: structure and bioactivity
    Bo Li
    School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, Henan, P R China
    Molecules 13:1671-95. 2008
  2. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
  3. pmc Carbohydrate-binding modules: fine-tuning polysaccharide recognition
    Alisdair B Boraston
    Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC, Canada V8W 3P6
    Biochem J 382:769-81. 2004
  4. ncbi A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds
    Albana Cumashi
    Department of Oncology and Neurosciences, University G D Annunzio Medical School and Foundation, 66013 Chieti, Italy, and Division of Immunogenetics, Hospital de Clinicas Jose de San Martin, Buenos Aires, Argentina
    Glycobiology 17:541-52. 2007
  5. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
  6. pmc Fucoidan extract induces apoptosis in MCF-7 cells via a mechanism involving the ROS-dependent JNK activation and mitochondria-mediated pathways
    Zhongyuan Zhang
    Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan
    PLoS ONE 6:e27441. 2011
  7. ncbi Ganoderma lucidum polysaccharides: immunomodulation and potential anti-tumor activities
    Zengtao Xu
    Institute of Chinese Medical Sciences, University of Macau, Macau, P R China
    Am J Chin Med 39:15-27. 2011
  8. pmc Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
    Ola Blixt
    Glycan Synthesis and Protein Expression Core D, Consortium for Functional Glycomics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 101:17033-8. 2004
  9. doi HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database software
    Louise Royle
    Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
    Anal Biochem 376:1-12. 2008
  10. ncbi Biomass recalcitrance: engineering plants and enzymes for biofuels production
    Michael E Himmel
    Chemical and Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA
    Science 315:804-7. 2007

Detail Information

Publications403 found, 100 shown here

  1. ncbi Fucoidan: structure and bioactivity
    Bo Li
    School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, Henan, P R China
    Molecules 13:1671-95. 2008
    ..This review summarizes the research progress on the structure and bioactivity of fucoidan and the relationships between structure and bioactivity...
  2. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
    ..consistent with this, antibodies able to block 2G12 binding to recombinant gp120 were not detected in significant quantities in 16 HIV-positive human serum samples...
  3. pmc Carbohydrate-binding modules: fine-tuning polysaccharide recognition
    Alisdair B Boraston
    Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC, Canada V8W 3P6
    Biochem J 382:769-81. 2004
    The enzymic degradation of insoluble polysaccharides is one of the most important reactions on earth...
  4. ncbi A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds
    Albana Cumashi
    Department of Oncology and Neurosciences, University G D Annunzio Medical School and Foundation, 66013 Chieti, Italy, and Division of Immunogenetics, Hospital de Clinicas Jose de San Martin, Buenos Aires, Argentina
    Glycobiology 17:541-52. 2007
    ..evaluation of P-selectin-mediated neutrophil adhesion to platelets under flow conditions revealed that only polysaccharides from Laminaria saccharina, L. digitata, Fucus evanescens, F. serratus, F. distichus, F...
  5. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
    ..This review discusses the increasingly sophisticated molecular mechanisms being discovered by which mammalian glycosylation governs physiology and contributes to disease...
  6. pmc Fucoidan extract induces apoptosis in MCF-7 cells via a mechanism involving the ROS-dependent JNK activation and mitochondria-mediated pathways
    Zhongyuan Zhang
    Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan
    PLoS ONE 6:e27441. 2011
    ..The present study was carried out to investigate the anti-tumor properties of FE in human carcinoma cells and further examine the underlying mechanisms of its activities...
  7. ncbi Ganoderma lucidum polysaccharides: immunomodulation and potential anti-tumor activities
    Zengtao Xu
    Institute of Chinese Medical Sciences, University of Macau, Macau, P R China
    Am J Chin Med 39:15-27. 2011
    ..lucidum, such as triterpenoids, polysaccharides, nucleosides, sterols, and alkaloids, among others. This paper examines the potential role of G...
  8. pmc Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
    Ola Blixt
    Glycan Synthesis and Protein Expression Core D, Consortium for Functional Glycomics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 101:17033-8. 2004
    ....
  9. doi HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database software
    Louise Royle
    Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
    Anal Biochem 376:1-12. 2008
    ..This technology is also suitable for preparing released glycans for other analytical techniques. Here we demonstrate its application to rheumatoid arthritis using 5 microl of patient serum...
  10. ncbi Biomass recalcitrance: engineering plants and enzymes for biofuels production
    Michael E Himmel
    Chemical and Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA
    Science 315:804-7. 2007
    ..To achieve sustainable energy production, it will be necessary to overcome the chemical and structural properties that have evolved in biomass to prevent its disassembly...
  11. doi The structure, function, and biosynthesis of plant cell wall pectic polysaccharides
    Kerry Hosmer Caffall
    University of Georgia, Department of Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, Athens, 30602, United States
    Carbohydr Res 344:1879-900. 2009
    ..There is a diversity of polysaccharides that make up the wall and that are classified as one of three types: cellulose, hemicellulose, or pectin...
  12. pmc Therapies from fucoidan; multifunctional marine polymers
    Janet Helen Fitton
    Marinova Pty Ltd, 249 Kennedy Drive, Cambridge, Tasmania 7170, Australia
    Mar Drugs 9:1731-60. 2011
    ..In recent years, the production of well characterized reproducible fucoidan fractions on a commercial scale has become possible making therapies from fucoidan a realizable goal...
  13. pmc Complex glycan catabolism by the human gut microbiota: the Bacteroidetes Sus-like paradigm
    Eric C Martens
    Center for Genome Sciences, Washington University School of Medicine, St Louis, Missouri 63108, USA
    J Biol Chem 284:24673-7. 2009
    ..These systems provide insights into how gut, as well as terrestrial and aquatic, Bacteroidetes survive in highly competitive ecosystems...
  14. pmc Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont
    Eric C Martens
    Center for Genome Sciences, Washington University School of Medicine, St Louis, MO 63108, USA
    Cell Host Microbe 4:447-57. 2008
    ..Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology...
  15. ncbi The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature
    Jocelyn K C Rose
    Department of Plant Biology, 228 Plant Science Building, Cornell University, Ithaca, NY 14853, USA
    Plant Cell Physiol 43:1421-35. 2002
    ....
  16. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  17. doi Fucoidans from brown seaweeds Sargassum hornery, Eclonia cava, Costaria costata: structural characteristics and anticancer activity
    Svetlana Ermakova
    Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia
    Appl Biochem Biotechnol 164:841-50. 2011
    ..The results clearly showed that fucoidans play an inhibitory role in colony formation in human melanoma and colon cancer cells and may be effective antitumor agents...
  18. pmc Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts
    Eric C Martens
    Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, Missouri, USA
    PLoS Biol 9:e1001221. 2011
    ..These polysaccharides are not digested by human enzymes, but are processed to absorbable short chain fatty acids by gut bacteria...
  19. ncbi Glycosylation and the immune system
    P M Rudd
    The Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Science 291:2370-6. 2001
    ..For example, in rheumatoid arthritis, an autoimmune disease, agalactosylated glycoforms of aggregated immunoglobulin G may induce association with the mannose-binding lectin and contribute to the pathology...
  20. pmc Variable loop glycan dependency of the broad and potent HIV-1-neutralizing antibodies PG9 and PG16
    Katie J Doores
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Virol 84:10510-21. 2010
    ..This information may be valuable for the design of immunogens to elicit PG9 and PG16-like antibodies, as well as constructs for cocrystallization studies...
  21. pmc Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens
    Katie J Doores
    Department of Immunology and Microbial Science and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:13800-5. 2010
    ..This study revises and extends our understanding of the glycan shield of HIV with implications for AIDS vaccine design...
  22. ncbi Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag
    Maria I Bilan
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii prosp 47, 119991 Moscow, Russian Federation
    Carbohydr Res 337:719-30. 2002
    ..NMR spectroscopy, and the data obtained were confirmed by methylation analysis of the native and desulfated polysaccharides. The fucoidan was shown to contain a linear backbone of alternating 3- and 4-linked alpha-L-fucopyranose 2-..
  23. ncbi A genomic view of the human-Bacteroides thetaiotaomicron symbiosis
    Jian Xu
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 299:2074-6. 2003
    ..proteome includes an elaborate apparatus for acquiring and hydrolyzing otherwise indigestible dietary polysaccharides and an associated environment-sensing system consisting of a large repertoire of extracytoplasmic function ..
  24. doi Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives
    Raj Kumar
    Radiation Biotechnology Unit, Division of Radiation Biology and Radiation Protection, Institute of Nuclear Medicine and Allied Sciences, New Delhi, 110054 India
    J Ind Microbiol Biotechnol 35:377-91. 2008
    ....
  25. ncbi Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides
    Angelina S Palma
    Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Northwick Park and St Mark s Campus, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
    J Biol Chem 281:5771-9. 2006
    ..zymosan, a particulate beta-glucan-rich extract of Saccharomyces cerevisiae, and binding is inhibited by polysaccharides rich in beta1,3- or both beta1,3- and beta1,6-linked glucose...
  26. ncbi Biosynthesis and assembly of capsular polysaccharides in Escherichia coli
    Chris Whitfield
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Annu Rev Biochem 75:39-68. 2006
    ..The remarkable structural diversity in capsular polysaccharides is illustrated by almost 80 capsular serotypes in Escherichia coli...
  27. doi Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Plant Biotechnol J 6:392-402. 2008
    ..The generated RNAi lines were stable, viable and did not show any obvious phenotype, thus providing a robust tool for the production of therapeutically relevant glycoproteins in plants with a humanized N-glycan structure...
  28. pmc N-glycosylation of colorectal cancer tissues: a liquid chromatography and mass spectrometry-based investigation
    Crina I A Balog
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 11:571-85. 2012
    ....
  29. pmc The antiviral activities and mechanisms of marine polysaccharides: an overview
    Wei Wang
    Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao 266003, China
    Mar Drugs 10:2795-816. 2012
    Recently, the studies on the antiviral activities of marine natural products, especially marine polysaccharides, are attracting more and more attention all over the world...
  30. doi An overview of mannan structure and mannan-degrading enzyme systems
    L R S Moreira
    Departamento de Biologia Celular, Laboratorio De Enzimologia, Universidade de Brasilia, CEP 70910 900 Brasilia, DF, Brazil
    Appl Microbiol Biotechnol 79:165-78. 2008
    ..Mannan-degrading enzymes have found applications in the pharmaceutical, food, feed, and pulp and paper industries. This review reports the structure of mannans and some biochemical properties and applications of mannan-degrading enzymes...
  31. pmc Role of complex carbohydrates in human immunodeficiency virus type 1 infection and resistance to antibody neutralization
    James M Binley
    Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121, USA
    J Virol 84:5637-55. 2010
    ....
  32. pmc Marine polysaccharides: a source of bioactive molecules for cell therapy and tissue engineering
    Karim Senni
    Seadev FermenSys SAS, Technopole Brest Iroise, France
    Mar Drugs 9:1664-81. 2011
    The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation ..
  33. pmc Rapid development of glycan-specific, broad, and potent anti-HIV-1 gp120 neutralizing antibodies in an R5 SIV/HIV chimeric virus infected macaque
    Laura M Walker
    Department of Immunology and Microbial Science and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:20125-9. 2011
    ....
  34. pmc Glycan labeling strategies and their use in identification and quantification
    L R Ruhaak
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300RC Leiden, The Netherlands
    Anal Bioanal Chem 397:3457-81. 2010
    ..In this review, various aspects of glycan labeling, separation, and detection strategies are discussed...
  35. pmc AXY8 encodes an α-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides
    Markus Günl
    University of California, Berkeley, California, 94720, USA
    Plant Cell 23:4025-40. 2011
    ..Furthermore, without the dominant apoplastic glycosylhydrolases, the XyG structure in the wall is mainly composed of XXXG and XXFG subunits...
  36. doi Plant cell wall polymers as precursors for biofuels
    Markus Pauly
    DOE Great Lakes Bioenergy Research Center, DOE Plant Research Lab, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    Curr Opin Plant Biol 13:305-12. 2010
    ..This new knowledge will be a valuable resource that can be used during efforts to generate designer biofuel crops using either selected breeding methods or recombinant DNA techniques...
  37. ncbi Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans
    Steven Bates
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Biol Chem 281:90-8. 2006
    ..This demonstrates the importance of N-glycan outer chain epitopes to the host-fungal interaction and virulence...
  38. pmc Mammalian glycosylation in immunity
    Jamey D Marth
    Department of Cellular and Molecular Medicine, The Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093, USA
    Nat Rev Immunol 8:874-87. 2008
    ..This Review focuses on the emerging immunological roles of the mammalian glycome...
  39. ncbi Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation
    Yoshikatsu Kaneko
    Laboratory of Molecular Genetics and Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 313:670-3. 2006
    ..This differential sialylation may provide a switch from innate anti-inflammatory activity in the steady state to generating adaptive pro-inflammatory effects upon antigenic challenge...
  40. ncbi Fucoidan induces apoptosis of human HS-sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways
    Yoshinobu Aisa
    Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan
    Am J Hematol 78:7-14. 2005
    ..These results demonstrate that fucoidan has direct anti-cancer effects on human HS-Sultan cells through caspase and ERK pathways...
  41. ncbi Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding
    Andrei J Petrescu
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U K
    Glycobiology 14:103-14. 2004
    ..Hydrophobic protein-glycan interactions and the low accessibility of glycosylation sites in folded proteins are common features and may be critical in mediating these functions...
  42. ncbi Heterofucans from Dictyota menstrualis have anticoagulant activity
    I R L Albuquerque
    Laboratório de Biotecnologia de Polímeros Naturais, Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, 59072 970 Natal, RN, Brazil
    Braz J Med Biol Res 37:167-71. 2004
    Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins...
  43. pmc Specificity of polysaccharide use in intestinal bacteroides species determines diet-induced microbiota alterations
    Erica D Sonnenburg
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 141:1241-52. 2010
    ..elucidate the basis of Bacteroides proliferation in response to fructans, a class of fructose-based dietary polysaccharides. Structural and genetic analysis disclosed a fructose-binding, hybrid two-component signaling sensor that ..
  44. doi Glycosylation, galectins and cellular signaling
    Cecile Boscher
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada
    Curr Opin Cell Biol 23:383-92. 2011
    ..Many important questions remain concerning the regulation of the galectins, the glycan ligands and lattice interaction with other membrane domains and endocytic pathways...
  45. doi N-Glycans in cancer progression
    Ken S Lau
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Glycobiology 18:750-60. 2008
    ..These studies suggest a systems approach to cancer treatment where the surface distribution of receptors is targeted through metabolism and N-glycan branching to induce growth arrest...
  46. pmc ARABINAN DEFICIENT 1 is a putative arabinosyltransferase involved in biosynthesis of pectic arabinan in Arabidopsis
    Jesper Harholt
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 140:49-58. 2006
    ..Analysis of cell wall polysaccharides isolated from leaves and stems showed that arabinose content was reduced to about 75% and 46%, respectively, ..
  47. doi GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans
    Alessio Ceroni
    Division of Molecular Biosciences, Imperial College London, London SW72AZ, UK
    J Proteome Res 7:1650-9. 2008
    ..The aim of GlycoWorkbench is to offer complete support for the routine interpretation of MS data. The software is available for download from: http://www.eurocarbdb.org/applications/ms-tools...
  48. ncbi Glycans in cancer and inflammation--potential for therapeutics and diagnostics
    Danielle H Dube
    Department of Chemistry, University of California, Berkeley, Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Rev Drug Discov 4:477-88. 2005
    ..This review highlights changes in glycosylation associated with cancer and chronic inflammation and new therapeutic and diagnostic strategies that are based on the underlying glycobiology...
  49. pmc Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers
    Lan Luo
    State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
    Mar Drugs 11:399-417. 2013
    In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers ..
  50. doi Mechanisms and principles of N-linked protein glycosylation
    Flavio Schwarz
    Institute of Microbiology, Department of Biology, ETH Zurich, 8093 Zurich, Switzerland
    Curr Opin Struct Biol 21:576-82. 2011
    ..Based on structural, functional, and phylogenetic approaches, this review discusses the highly conserved processes that are at the basis of this unique general protein modification system...
  51. doi N-glycomic biomarkers of biological aging and longevity: a link with inflammaging
    Fabio Dall'olio
    Department of Experimental Pathology, University of Bologna, Via S Giacomo, 14 I 40126 Bologna, Italy
    Ageing Res Rev 12:685-98. 2013
    ..Thus, altered N-glycans are both powerful markers of aging and possible contributors to its pathogenesis...
  52. ncbi Apoptosis inducing activity of fucoidan in HCT-15 colon carcinoma cells
    Jae Hee Hyun
    School of Medicine, Institute of Medical Sciences, Jeju National University, South Kore
    Biol Pharm Bull 32:1760-4. 2009
    ..These data support the hypothesis that fucoidan may have potential in colon cancer treatment...
  53. pmc Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions
    Inka Brockhausen
    Department of Medicine and Biochemistry, Human Mobility Research Centre, Queen s University, Kingston General Hospital, Angada 1, Kingston, Ontario K7L 2V7, Canada
    EMBO Rep 7:599-604. 2006
    ..This review focuses on the glycosyltransferases of colon and breast cancer cells that determine the pathways of mucin-type O-glycosylation, and the proposed functional and pathological consequences of altered O-glycans...
  54. doi ESIMS analysis of fucoidan preparations from Costaria costata, extracted from alga at different life-stages
    Stanislav D Anastyuk
    GB Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp 159, 690022 Vladivostok, Russian Federation
    Carbohydr Polym 90:993-1002. 2012
    ..Generative alga in general produced highly sulfated galactofucan having lower MW along with less sulfated mannoglucuronofucan with higher MW, which was extensively synthesized by vegetative algae...
  55. pmc Fucanomics and galactanomics: marine distribution, medicinal impact, conceptions, and challenges
    Vitor H Pomin
    Program of Glycobiology, Institute of Medical Biochemistry, and University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941 590, Brazil
    Mar Drugs 10:793-811. 2012
    ..Sulfated fucans (SFs) and sulfated galactans (SGs) are relatively new classes of sulfated polysaccharides (SPs) that occur mostly in marine organisms, and exhibit a broad range of medicinal effects...
  56. doi Alternative glycosylation modulates function of IgG and other proteins - implications on evolution and disease
    Olga Gornik
    University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia
    Biochim Biophys Acta 1820:1318-26. 2012
    ..However, contrary to proteins which are defined by their individual genetic templates, glycans are encoded in a complex dynamic network of hundreds of genes which participate in the complex biosynthetic pathway of protein glycosylation...
  57. doi Elimination of β-mannose glycan structures in Pichia pastoris
    Daniel Hopkins
    GlycoFi Inc, a Wholly Owned Subsidiary of Merck and Co Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Glycobiology 21:1616-26. 2011
    ..Taken together, the elimination of β-Man containing glycoforms represents an important step forward for the Pichia production platform as a suitable system for the production of therapeutic glycoproteins...
  58. pmc Bacillus subtilis biofilm induction by plant polysaccharides
    Pascale B Beauregard
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:E1621-30. 2013
    ..B. subtilis biofilm formation was triggered by certain plant polysaccharides. These polysaccharides served as a signal for biofilm formation transduced via the kinases controlling the ..
  59. doi Inhibitory effect of Astragalus polysaccharides on lipopolysaccharide-induced TNF-a and IL-1β production in THP-1 cells
    Xiaojuan He
    Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
    Molecules 17:3155-64. 2012
    Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown...
  60. pmc Nasal Acai polysaccharides potentiate innate immunity to protect against pulmonary Francisella tularensis and Burkholderia pseudomallei Infections
    Jerod A Skyberg
    Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, USA
    PLoS Pathog 8:e1002587. 2012
    ..Several natural agonists were screened for their ability to enhance host resistance to infection, and polysaccharides derived from the Acai berry (Acai PS) were found to have potent abilities as an immunotherapeutic to treat F...
  61. doi Fucoidan from Undaria pinnatifida induces apoptosis in A549 human lung carcinoma cells
    Hye Jin Boo
    School of Medicine, Institute of Medical Sciences, Jeju National University, 66 Jejudaehakno, Jeju 690 756, South Korea
    Phytother Res 25:1082-6. 2011
    ..Together, these results indicate that fucoidan induces apoptosis of A549 human lung cancer cells through down-regulation of p38, PI3K/Akt, and the activation of the ERK1/2 MAPK pathway...
  62. doi Plant cell walls to ethanol
    Douglas B Jordan
    USDA Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL 61604, U S A
    Biochem J 442:241-52. 2012
    ....
  63. pmc A unique beta1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, BOKU, University of Natural Resources and Applied Life Sciences, A 1190 Viena, Austria
    Plant Cell 19:2278-92. 2007
    ....
  64. doi Structure and interactions of plant cell-wall polysaccharides by two- and three-dimensional magic-angle-spinning solid-state NMR
    Marilú Dick-Pérez
    Department of Chemistry and Ames Laboratory, Iowa State University, Ames, Iowa 50011, United States
    Biochemistry 50:989-1000. 2011
    ..NMR and uniformly (13)C-labeled Arabidopsis thaliana, we have assigned the resonances of the major polysaccharides in the intact and insoluble primary CW and determined the intermolecular contacts and dynamics of cellulose, ..
  65. ncbi The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell
    L B Talarico
    Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
    Antiviral Res 66:103-10. 2005
    Two homogeneous sulfated polysaccharides obtained from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata, the kappa/iota/nu carrageenan G3d and the dl-galactan hybrid C2S-3, were assayed for their antiviral properties ..
  66. pmc Chemical structures and bioactivities of sulfated polysaccharides from marine algae
    Guangling Jiao
    National Research Council Canada, Institute for Marine Biosciences, Halifax, NS, B3H 3Z1, Canada
    Mar Drugs 9:196-223. 2011
    Sulfated polysaccharides and their lower molecular weight oligosaccharide derivatives from marine macroalgae have been shown to possess a variety of biological activities...
  67. ncbi Lycium barbarum polysaccharides: Protective effects against heat-induced damage of rat testes and H2O2-induced DNA damage in mouse testicular cells and beneficial effect on sexual behavior and reproductive function of hemicastrated rats
    Qiong Luo
    College of Public Health, Wuhan University, Wuhan 430071, Hubei, PR China
    Life Sci 79:613-21. 2006
    ..b>Polysaccharides are the most important functional constituent in L. barbarum fruits...
  68. ncbi Hypoglycemic effect of Astragalus polysaccharide and its effect on PTP1B
    Yong Wu
    Department of Pathophysiology, Medical College of Wuhan University, Wuhan 430060, China
    Acta Pharmacol Sin 26:345-52. 2005
    ....
  69. pmc Removal of a single N-linked glycan in human immunodeficiency virus type 1 gp120 results in an enhanced ability to induce neutralizing antibody responses
    Yun Li
    Washington National Primate Center, University of Washington, 3000 Western Ave, Seattle, WA 98121, USA
    J Virol 82:638-51. 2008
    ..These results indicate the potential advantages for glycan modification in vaccine design, although the role of specific glycans requires further examination...
  70. ncbi The glycosylation and structure of human serum IgA1, Fab, and Fc regions and the role of N-glycosylation on Fcα receptor interactions
    T S Mattu
    Department of Biochemistry, Oxford University, United Kingdom
    J Biol Chem 273:2260-72. 1998
    ..This also suggests why in contrast to IgG Fc, the IgA1 N-glycans are not undergalactosylated in rheumatoid arthritis...
  71. doi Fucoidan induces apoptosis through activation of caspase-8 on human breast cancer MCF-7 cells
    Yumi Yamasaki-Miyamoto
    Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6 10 1 Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
    J Agric Food Chem 57:8677-82. 2009
    ..However, caspase-9-specific inhibitor exerted a moderate inhibitory effect on the cytotoxicity of fucoidan. These data indicated that fucoidan could induce apoptotic cell death through a caspase-8-dependent pathway in MCF-7 cells...
  72. pmc The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade
    Camille Bonomelli
    Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, United Kingdom
    PLoS ONE 6:e23521. 2011
    ..This study highlights differences in glycosylation between virion-associated and recombinant gp120...
  73. pmc Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana
    Eva Liebminger
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, A 1190 Viena, Austria
    Plant Cell 21:3850-67. 2009
    ..These findings show that class I alpha-mannosidases are essential for early N-glycan processing and play a role in root development and cell wall biosynthesis in Arabidopsis...
  74. ncbi Glycans and neural cell interactions
    Ralf Kleene
    Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
    Nat Rev Neurosci 5:195-208. 2004
    ..The tremendous structural diversity of glycan chains allows for immense combinatorial possibilities that might underlie the fine-tuning of cell-cell and cell-matrix interactions...
  75. doi Inhibitory effects of fucoidan on activation of epidermal growth factor receptor and cell transformation in JB6 Cl41 cells
    Na Yeon Lee
    College of Pharmacy, Chosun University, Gwangju, South Korea
    Food Chem Toxicol 46:1793-800. 2008
    ..Taken together, these results indicate that fucoidan might exert chemopreventive effects through the inhibition of phosphorylation of the EGFR...
  76. ncbi Complex N-glycan number and degree of branching cooperate to regulate cell proliferation and differentiation
    Ken S Lau
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Cell 129:123-34. 2007
    ..We have identified a mechanism for metabolic regulation of cellular transition between growth and arrest in mammals arising from apparent coevolution of N-glycan number and branching...
  77. pmc Glycomics profiling of Chinese hamster ovary cell glycosylation mutants reveals N-glycans of a novel size and complexity
    Simon J North
    Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 285:5759-75. 2010
    ..This analysis reveals the larger-than-expected complexity of N-glycans in CHO cell mutants that may be used in a broad variety of functional glycomics studies and for making recombinant glycoproteins...
  78. doi Biological activities of sulfated polysaccharides from tropical seaweeds
    L S Costa
    UFRN, Laboratório de Biotecnologia de Polímeros Naturais Biopol, Centro de Biociencias, Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, Av Sen Salgado Filho, 3000, 59072970 Natal, Rio Grande do Norte, Brazil
    Biomed Pharmacother 64:21-8. 2010
    Sulfated polysaccharides from 11 species of tropical marine algae (one edible specie of Rhodophyta, six species of Phaeophyta and four species of Chlorophyta) collected from Natal city coast (Northeast of Brazil) were evaluated for their ..
  79. pmc Protective effect of ginseng polysaccharides on influenza viral infection
    Dae Goon Yoo
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, United States of America
    PLoS ONE 7:e33678. 2012
    ..Daily treatment of vaccinated mice with GP improved their survival against heterosubtypic lethal challenge. This study demonstrates the first evidence that GP can be used as a remedy against influenza viral infection...
  80. pmc Copper-free click chemistry for dynamic in vivo imaging
    Jeremy M Baskin
    Department of Chemistry, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:16793-7. 2007
    ..With this technique, we studied the dynamics of glycan trafficking and identified a population of sialoglycoconjugates with unexpectedly rapid internalization kinetics...
  81. pmc Polysaccharides from wolfberry prevents corticosterone-induced inhibition of sexual behavior and increases neurogenesis
    Benson Wui Man Lau
    Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, People s Republic of China
    PLoS ONE 7:e33374. 2012
    ..The aim of this study is to determine the effect of Lycium barbarum polysaccharides (LBP) on male sexual behavior of rats...
  82. doi Dietary roles of non-starch polysaccharides in human nutrition: a review
    Vikas Kumar
    Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim 70599, Stuttgart, Germany
    Crit Rev Food Sci Nutr 52:899-935. 2012
    Nonstarch polysaccharides (NSPs) occur naturally in many foods. The physiochemical and biological properties of these compounds correspond to dietary fiber...
  83. pmc Anticancer properties and mechanisms of fucoidan on mouse breast cancer in vitro and in vivo
    Meilan Xue
    Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao, China
    PLoS ONE 7:e43483. 2012
    ..In this study, we examined the influence of crude fucoidan on mouse breast cancer in vitro and in vivo...
  84. ncbi Structure, assembly and regulation of expression of capsules in Escherichia coli
    C Whitfield
    Department of Microbiology, University of Guelph, Ontario, Canada
    Mol Microbiol 31:1307-19. 1999
    ..Capsular polysaccharides represent a major surface antigen, the K antigen, and more than 80 distinct K serotypes result from ..
  85. doi The cell wall polysaccharide metabolism of the brown alga Ectocarpus siliculosus. Insights into the evolution of extracellular matrix polysaccharides in Eukaryotes
    Gurvan Michel
    UPMC University Paris 6, UMR 7139 Marine Plants and Biomolecules, Station Biologique de Roscoff, F 29682 Roscoff, Bretagne, France
    New Phytol 188:82-97. 2010
    ..some components with plants (cellulose) and animals (sulfated fucans), but they also contain some unique polysaccharides (alginates)...
  86. pmc Polysaccharides cellulose, poly-beta-1,6-n-acetyl-D-glucosamine, and colanic acid are required for optimal binding of Escherichia coli O157:H7 strains to alfalfa sprouts and K-12 strains to plastic but not for binding to epithelial cells
    Ann G Matthysse
    Department of Biology, Coker Hall, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Appl Environ Microbiol 74:2384-90. 2008
    ..coli O157:H7 to sprouts, the polysaccharides required for binding are not redundant and each polysaccharide may play a distinct role...
  87. doi Identification of two polysaccharides from Prunella vulgaris L. and evaluation on their anti-lung adenocarcinoma activity
    Liang Feng
    Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, China
    Molecules 15:5093-103. 2010
    ..for tumor therapy in Traditional Chinese Medicine for centuries, however, systematic research on extracted PV polysaccharides believed to possess various biological activities, as well as their preventive and anti-tumor effects on lung ..
  88. pmc In vivo imaging of membrane-associated glycans in developing zebrafish
    Scott T Laughlin
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 320:664-7. 2008
    ..Using a multicolor detection strategy, we performed a spatiotemporal analysis of glycan expression and trafficking and identified patterns that would be undetectable with conventional molecular imaging approaches...
  89. pmc Sub-compartmental organization of Golgi-resident N-glycan processing enzymes in plants
    Jennifer Schoberer
    School of Life Sciences, Oxford Brookes University, Headington Campus, Gipsy Lane, Oxford OX30BP, UK
    Mol Plant 4:220-8. 2011
    ..Yet, the signals, mechanism(s), and molecular factors involved are still unknown. Here, we address recent findings and summarize the current understanding of this fundamental process in plant cell biology...
  90. doi Antitumor and anti-inflammatory activities of polysaccharides isolated from Ganoderma lucidum
    Soniamol Joseph
    Phytochemistry and Phytopharmacology Division, Tropical Botanic Garden and Research Institute, Palode Thiruvananthapuram 695562, India
    Acta Pharm 61:335-42. 2011
    In this study, polysaccharides were isolated from Ganoderma lucidum (Polyporaceae) and their antitumor and anti-inflammatory activities were investigated using in vivo models. Potential antitumor activity was shown by G...
  91. doi Therapeutic effects of astragalus polysaccharides on inflammation and synovial apoptosis in rats with adjuvant-induced arthritis
    Jun Bing Jiang
    College of Veterinary Medicine and Animal Science, Shanxi Agricultural University, Taigu
    Int J Rheum Dis 13:396-405. 2010
    ..This study aims to evaluate therapeutic effects and the mechanisms of astragalus polysaccharides (APS) on adjuvant-induced arthritis (AA) in rats.
  92. pmc Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula
    Leandro Silva Costa
    Laboratório de Biotecnologia de Polímeros Naturais Biopol, Departamento de Bioquimica, Centro de Biociencias, Universidade Federal do Rio Grande do Norte UFRN, Natal, Rio Grande do Norte, Brazil
    Mar Drugs 9:952-66. 2011
    ..69 and 13.83 μM, respectively. These results clearly indicate the beneficial effect of S. filipendula polysaccharides as antiproliferative and antioxidant...
  93. pmc Carbohydrate-binding modules promote the enzymatic deconstruction of intact plant cell walls by targeting and proximity effects
    Cecile Herve
    Faculty of Biological Sciences, Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
    Proc Natl Acad Sci U S A 107:15293-8. 2010
    ..These studies demonstrate that CBMs can potentiate the action of a cognate catalytic module toward polysaccharides in intact cell walls through the recognition of nonsubstrate polysaccharides...
  94. doi Aspergillus fumigatus: cell wall polysaccharides, their biosynthesis and organization
    Amandine Gastebois
    Unite des Aspergillus, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724, Paris, Cedex 15, France
    Future Microbiol 4:583-95. 2009
    ..The cell wall, in the majority of fungi, is composed of polysaccharides, and understanding the biochemical organization and biogenesis of an A...
  95. doi Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica
    Jing Wang
    Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
    Int J Biol Macromol 46:6-12. 2010
    ..Available data presented the content of sulfate group, the molar ratio of sulfate/fucose and sulfate/total sugar, and the molecular weight played an important role on antioxidant and anticoagulant activity...
  96. pmc Evidence for branching in cryptococcal capsular polysaccharides and consequences on its biological activity
    Radames J B Cordero
    Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA
    Mol Microbiol 79:1101-17. 2011
    ..neoformans PS...
  97. ncbi Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides
    D L Topping
    Commonwealth Scientific and Industrial Research Organization, Health Sciences and Nutrition, Adelaide, Australia
    Physiol Rev 81:1031-64. 2001
    ..Human colonic bacteria ferment RS and nonstarch polysaccharides (NSP; major components of dietary fiber) to short-chain fatty acids (SCFA), mainly acetate, propionate, and ..
  98. doi An extended set of monoclonal antibodies to pectic homogalacturonan
    Yves Verhertbruggen
    Centre for Plant Sciences, University of Leeds, United Kingdom
    Carbohydr Res 344:1858-62. 2009
    ..The epitopes bound by these antibodies display some similarities and some differences to the epitopes recognized by the previously isolated and established pectic homogalacturonan probes JIM5 and JIM7...
  99. doi Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis
    Harry J Flint
    Microbial Ecology Group, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
    Nat Rev Microbiol 6:121-31. 2008
    The microbiota of the mammalian intestine depend largely on dietary polysaccharides as energy sources...
  100. ncbi Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases
    Florence Goubet
    Department of Biochemistry, Cambridge University, Building O, Downing Site, Cambridge, CB2 1QW, United Kingdom
    Anal Biochem 300:53-68. 2002
    A method to characterize plant cell wall polysaccharides is presented...
  101. ncbi The sweet and sour of cancer: glycans as novel therapeutic targets
    Mark M Fuster
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of California, San Diego, La Jolla, California, 92093 0687, USA
    Nat Rev Cancer 5:526-42. 2005
    ..Such novel agents might be used alone or in combination with operative and/or chemoradiation strategies for treating cancer...

Research Grants73

  1. Novel Bioconjugate Vaccines to Prevent Staphylococcus aureus Infection
    JEAN CLAIRE LEE; Fiscal Year: 2013
    ..coli for the tetravalent bioconjugate. By glyco-engineering technology, capsular polysaccharides are conjugated to known protective peptides without denaturing the protein carrier, thus conserving B cell ..
  2. Biofilm Infections in Postsurgical, Trauma, and Critically Ill Patients
    Carol L Wells; Fiscal Year: 2013
    ..surrounded by a complex extracellular polymeric substance composed of proteins, glycoproteins, glycolipids, polysaccharides, and DNA...
  3. Localization and characterization of the Clostridium difficile biofilm
    Adam Driks; Fiscal Year: 2013
    ..Using laser-scanning confocal microscopy, we found that polysaccharides and nucleic acids are present in the matrix...
  4. GENETICS AND VIRULENCE OF PNEUMOCOCCAL CAPSULAR TYPES
    JANET L YOTHER; Fiscal Year: 2012
    ..Essential to the ability to cause disease are the capsular polysaccharides. Although 91 distinct capsular serotypes have been identified, all perform similar functions...
  5. Novel Glycosaminoglycan Ethers for Prevention of Metastasis
    Alana L Welm; Fiscal Year: 2011
    ..GlycoMira has developed a family of polyanionic, metabolically stabilized polysaccharides, the semi- synthetic glycosaminoglycan ethers (SAGEs)...
  6. Biofilm matrix-degrading enzymes for the treatment and prevention of S. aureus-as
    Jeffrey B Kaplan; Fiscal Year: 2013
    ..The extracellular matrix of most biofilms contains polymers such as polysaccharides and DNA...
  7. The Role of Syntrophic Bacteria in Methanogenic Metabolism in the Human Gut
    Catherine Lozupone; Fiscal Year: 2013
    ..g. complex plant polysaccharides)...
  8. Target discovery and immunoassay for diagnosis of invasive aspergillosis
    Thomas R Kozel; Fiscal Year: 2013
    ..for the InMAD approach to target discovery is already in hand for tularemia, melioidosis and relapsing fever where candidate proteins and polysaccharides that are shed into serum during infection have been identified.
  9. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng George Wang; Fiscal Year: 2013
    ..Surface-located Polysaccharides (PSs) of bacteria have great potentials to be used as vaccines for preventing bacterial infections...
  10. Novel Glycosaminoglycan Derivatives for Treatment of Bladder Inflammation
    JUSTIN RODNEY SAVAGE; Fiscal Year: 2011
    ..Two anti-inflammatory sulfated polysaccharides are currently used for therapy, but neither is particularly effective...
  11. Toll-like receptor signaling in the generation of B1b cell memory
    Kishore R Alugupalli; Fiscal Year: 2012
    ..B cell responses to T cell-independent antigens such as bacterial polysaccharides are generated primarily by cross-linking B cell antigen receptors (BCR)...
  12. Bordetella Biofilms and Pathogenesis
    RAJENDAR K DEORA; Fiscal Year: 2011
    ..Bps is similar to PGA/PIA/PNAG (poly-2-1,6-N- acetylglucosamine) polysaccharides of other bacteria including a number of pathogens...
  13. Multi-specific Antibody Therapy by targeting S. aureus toxins and polysaccharides
    M Javad Aman; Fiscal Year: 2013
    ..The pathogenicity of S. aureus is dependent on many virulence factors, including cell surface proteins and polysaccharides, as well as secreted toxins that cause tissue damage, promote bacterial dissemination and metastatic growth ..
  14. Interactions between Bacterial Tyrosine Kinases and Phosphatases
    Ranajeet Ghose; Fiscal Year: 2013
    ..BY-kinase mediated signal transduction plays a central role in the synthesis of polysaccharides responsible for capsule and biofilm formation, the former acting as antiphagocytic virulence factors for many ..
  15. Mechanism of cellulose synthesis and transport across biological membranes
    Jochen Zimmer; Fiscal Year: 2013
    ..are best described as multi-cellular, usually sessile, bacterial aggregates stabilized by extracellular polysaccharides and are implicated in a number of pathogenic conditions...
  16. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2011
    ..g. spatial distribution of biofilm matrix and fungi, and linkage composition of the polysaccharides. This training will provide expertise in virology, structure determination, and cell culture techniques...
  17. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2013
    ..g. spatial distribution of biofilm matrix and fungi, and linkage composition of the polysaccharides. This training will provide expertise in virology, structure determination, and cell culture techniques...
  18. Mechanisms of Mycoplasmal Disease Pathogenesis
    KEVIN F DYBVIG; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The surface of virtually all bacterial pathogens is coated with polysaccharides (exopolysaccharides, EPS) that can protect bacteria from host defenses and modulate the ability of the bacteria to ..
  19. V. cholerae proteins are required for biofilm growth-vaccine targets
    HAVVA FITNAT YILDIZ; Fiscal Year: 2011
    ..Biofilms are complex structures composed of bacterial cells, proteins, and polysaccharides which allow the bacteria to exist in hostile environments...
  20. SEQUENCING THE POLYSACCHARIDE COMPONENT OF PROTEOGLYCANS
    Robert J Linhardt; Fiscal Year: 2012
    ..Currently it is difficult to analyze and not possible to sequence these complex polysaccharides. This has prevented a firm understanding of the structure- activity relationship of this important class of ..
  21. Elderly Immune Response to Pneumococcal Polysaccharide
    M A JULIA WESTERINK; Fiscal Year: 2010
    ..aging affects the B cell clonotype and frequency of somatic mutation in the immune response to pneumococcal polysaccharides and that these changes result in altered antibody avidity and functional activity...
  22. Mechanism of HigB-mediated ribosome-dependent mRNA degradation
    MARC ANTHONY SCHURECK; Fiscal Year: 2013
    ..adhesive and are protected from antibiotics by a self-produced extracellular matrix of DNA, protein and polysaccharides. Therefore, it is critical to have a fundamental understanding of the pathways leading to this antibiotic-..
  23. Impact of Diet on Intestinal Microbiota-Host Dynamics
    Justin Sonnenburg; Fiscal Year: 2013
    ..theta), devote vast portions of their genomes to the utilization of undigested dietary plant polysaccharides (i.e., dietary fiber)...
  24. Lectin-Carbohydrate Interactions in the Host-Parasite System
    Xiao Qiang Yu; Fiscal Year: 2010
    ..subalbatus. Identify surface proteins or surface polysaccharides of C. elegans and B. malayi that can be recognized by Manduca immulectin-2 or Armigeres C-type lectins...
  25. REGULATION OF PROTECTIVE HUMORAL IMMUNITY TO STREPTOCOCCUS PNEUMONIAE BY PD-1
    Karen M Haas; Fiscal Year: 2012
    ..receptor and its ligands as a strategy to enhance protective antibody responses to pneumococcal polysaccharides (PPSs) found in the capsule of Streptococcus pneumoniae. S...
  26. Live Attenuated Recombinant Bacterial Delivery of Polysaccharide Vaccine Antigens
    Joanna B Goldberg; Fiscal Year: 2010
    Bacterial polysaccharides are excellent targets for vaccine development as they are surface exposed and often the most protective antigens expressed on a pathogen...
  27. Polysaccharide Ecocomposite Materials: Recyclable Synthesis and Applications
    Chieu D Tran; Fiscal Year: 2012
    ..which limit synthesis and applications of composite materials based on the two most abundant biorenewable polysaccharides, cellulose (CEL) and chitotan (CS)...
  28. Synthesis and properties of a bacterial bioadhesive
    Yves V Brun; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Bacteria often utilize polysaccharides as adhesive structures to attach to surfaces, to form biofilms, and to infect host cells...
  29. UAB Multi-user Fermentation Facility Upgrade
    NORMAN P HIGGINS; Fiscal Year: 2010
    ..The needs of this group included isolating enzymes, lipids, nucleic acids, and polysaccharides from a range of cultured organisms including bacteria, yeast, and tissue culture cells...
  30. Effects of neonatal microbial exposure on anti-polysaccharide B cell development
    JOHN FRANKLIN KEARNEY; Fiscal Year: 2013
    ..pursued by three aims: 1) to identify factors affecting the establishment of the B cell clonal repertoire to polysaccharides expressed by S. pneumoniae, S. pyogenes (GAS), S. agalactiae (GBS) and E...
  31. Antigen processing of pneumococcal conjugate vaccines
    John R Schreiber; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Pneumococcal (Pn) capsular polysaccharides (PS) are T independent antigens that do not induce memory responses and are poor immunogens in infants...
  32. Biosynthesis & Function of a Bacillus anthracis-specific cell wall polysaccharide
    Russell W Carlson; Fiscal Year: 2010
    ..Preliminary data and results in the literature support that these polysaccharides could be crucial for important functions with regard to the growth and virulence of pathogenic Bacillus ..
  33. New Frontiers at the Interface of Immunity and Glycobiology
    Andrew D Robertson; Fiscal Year: 2011
    ..our understanding of the innate immune response, while other findings reveal that glycolipids and bacterial polysaccharides can lead to NKT and T cell activation...
  34. Assembly and Function of the Yeast Spore Wall
    Aaron M Neiman; Fiscal Year: 2013
    ..The cell wall is composed of interlocked proteins and polysaccharides and serves as a buffer between the cell and its environment...
  35. Regulation of Virulence of Cryptococcus neoformans
    Hiten D Madhani; Fiscal Year: 2013
    ..Thus, we will test the hypothesis that Gat201 activates the production of chitin and/or chitin-derived polysaccharides and that these are necessary for C...
  36. Symbiosis of E. coli and the Intestinal Microbiota in a Mouse Model
    Tyrrell Conway; Fiscal Year: 2013
    ..coli. Since E. coli is unable to hydrolyze complex polysaccharides, which are the primary nutrient source in the gut, and because E...
  37. Crystallization of outer membrane proteins for export of polysaccharide capsule
    Mark A Saper; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Bacteria often export high molecular weight polysaccharides that can form a capsule associated with the bacterial surface, or be secreted into the surrounding environment as exopolysaccharide...
  38. Countermeasure for Chlorine Inhalation
    SIGRIDUR G SUMAN; Fiscal Year: 2011
    ..cells to form a variety of oxidized species, mainly chloramines and oxidized sulfur, on proteins and polysaccharides. Some oxidation is accompanied by damaging changes to enzymes and key cellular components through free-..
  39. PROTEOGLYCANS IN NORMAL AND SCARRED CORNEAS
    James L Funderburgh; Fiscal Year: 2012
    ..will focus on the biosynthesis and biological functions of stromal glycosaminoglycans (GAGs), sulfated polysaccharides linked to proteoglycan proteins...
  40. MOLECULAR MECHANISMS OF HISTOPLASMA PATHOGENESIS
    William E Goldman; Fiscal Year: 2011
    ..period was focused on identifying and characterizing Histoplasma yeast phase-specific genes, proteins, and polysaccharides. Two of these, CBP (a secreted protein) and α-(1,3)-glucan (a cell wall polysaccharide), became the ..
  41. The role of polysaccharide surface capsules in Bacteroides glycan degradation
    Eric C Martens; Fiscal Year: 2013
    ..These species ubiquitously produce multiple capsular polysaccharides (CPS) on their cell surfaces...
  42. CD1d and NKT Cell Activation and Function in Infection
    Michael B Brenner; Fiscal Year: 2013
    ..data reveal that fungi strikingly activate iNKT cells and appear to use a mechanism mediated by complex polysaccharides in their cells walls such as the characteristic 2-glucans, rather than fungal lipid antigens...
  43. Complex glycan utilization by human gut Bacteroides
    Eric C Martens; Fiscal Year: 2013
    ..RELEVANCE (See instructions): Human gut bacteria are essential for the transformation of complex dietary polysaccharides, many of which we cannot digest on our own, into forms that we readily absorb...
  44. Biosynthesis of Polysaccharides
    Peng George Wang; Fiscal Year: 2012
    ..In spite of the remarkable structural diversity of polysaccharides, only three major elongation/polymerization mechanisms, namely wzy- dependent, ABC-transporter dependent and ..
  45. Enzymatic Therapies for Cystic Fibrosis Biofilms
    PHILLIP J BRUMM; Fiscal Year: 2011
    ..is composed of bacterial cells surrounded by a three dimensional matrix of diverse substances, including polysaccharides, proteins, nucleic acids, and lipids;the major structural component appears to be polysaccharides produced by ..
  46. CAPSULE REGULATION AND VIRULENCE IN CRYPTOCOCCUS NEOFORMANS
    Tamara L Doering; Fiscal Year: 2013
    ..neoformans is an extensive polysaccharide capsule. The structures of the capsule polysaccharides are known and aspects of capsule construction have been studied, but there is only limited understanding of ..
  47. Antibody immunity to serotype 3 Streptococcus pneumoniae
    Liise Anne Pirofski; Fiscal Year: 2013
    ..disease (IPD) changed markedly after the introduction in 2000 of a protein conjugate vaccine with capsular polysaccharides (PPS) of seven of the most common disease-causing serotypes (ST) of Streptococcus pneumoniae (pneumococcus) (..
  48. Ginseng and its Constituents in Complementary Breast Cancer Therapy
    Laura Murphy; Fiscal Year: 2010
    ..We provide preliminary evidence that ginseng polysaccharides stimulate the innate immune system and induce macrophages to produce increased cytokine release...
  49. Molecular Mechanisms of L. monocytogenes Adhesion Through Surface Biopolymers
    NEHAL IBRAHIM ABU LAIL; Fiscal Year: 2010
    ..has been formulated in part due to my earlier work that pointed to the importance of bacterial surface polysaccharides in controlling the attachment of bacteria to surfaces...
  50. Nanomechanics of the extracellular matrix
    Julio M Fernandez; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The extracellular matrix is a complex web of proteins and polysaccharides that provides the mechanical scaffold that guides the development and preserves the integrity of organs and tissues...
  51. Genomic and metabolomic foundations of human-microbial symbiosis in the gut
    JEFFREY IVAN GORDON; Fiscal Year: 2013
    ..traits we have not had to evolve on our own, including the ability to process otherwise indigestible dietary polysaccharides. This grant renewal focuses on characterizing the genomic and "metabolomic" features of the microbiota's ..
  52. Swarming as a model for surface-sensing in Salmonella typhimurium
    Rasika M Harshey; Fiscal Year: 2012
    ..Swarmer cells generally make more flagella, and excrete surfactants and polysaccharides that aid surface motility by generating wetness and providing lubrication...
  53. RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION
    Russell W Carlson; Fiscal Year: 2010
    ..These adaptations involve changes to cell surface polysaccharides, such as lipopolysaccharides (LPSs) and capsular polysaccharides...
  54. CD1d-restricted NKT cells in microbial infection
    Manfred Brigl; Fiscal Year: 2012
    ..pneumoniae (Aim 1). In addition, we will isolate lipids and lipidated polysaccharides from S...
  55. PGT Inhibitors Mapped From a Tunicamycin Blueprint
    Barbara Imperiali; Fiscal Year: 2013
    ..many glycoconjugate assembly pathways including those that lead to bacterial lipopolysaccharides and capsular polysaccharides, teichoic acids, and N- and O-linked glycoproteins...
  56. Evaluation of a Novel Anti-Caries Approach to Modulate Virulence of S. mutans
    Hyun Koo; Fiscal Year: 2012
    ..The extracellular polysaccharides (EPS) are synthesized by microorganisms (Streptococcus mutans, a key contributor) and promote biochemical and ..
  57. Mycoplasma Polysaccharides and Control of Infection
    KEVIN F DYBVIG; Fiscal Year: 2012
    ..We believe that M. pulmonis and M. pneumoniae both produce at least two polysaccharides that are critical for pathogenesis...
  58. Structures, Functions, &Genetic Diversity of Oligosaccharides in Milk
    Carlito B Lebrilla; Fiscal Year: 2013
    ..However, despite advances in the commercialization of simple plant oligosaccharides and polysaccharides, these products of plant metabolism are unable to substantially alter the human gut microbial ecology nor its ..
  59. Protective Human Anti-Candida Antibodies
    Mason X Zhang; Fiscal Year: 2013
    ..The cell surface of C. albicans is enriched with manose oligosaccharides and polysaccharides known as mannan that plays an important role in Candida-human interactions...
  60. Novel antibacterial agents resistant to inactivation by polyelectrolytes
    Paul A Janmey; Fiscal Year: 2010
    ..and mucins, and the bacterial-derived polyanions lipopolysaccharide (LPS), lipoteichoic acid (L TA) and polysaccharides such as alginate...
  61. Interactions of a commensal bacterium with the intestinal mucosal surface
    Breck A Duerkop; Fiscal Year: 2012
    ..These organisms make critical contributions to human metabolism by helping to break down complex polysaccharides that are ingested as part of the diet...
  62. Glycomics of Heparan Sulfate in Bacterial Pathogenesis
    Pyong Woo Park; Fiscal Year: 2010
    ..We will determine the effects of 3-O-sulfation on the capacity of HS polysaccharides to regulate soluble antimicrobial factors, CXC chemokine-induced neutrophil recruitment, and anti-bacterial ..
  63. Sulfated Polysaccharide Derivatives for the Treatment of Rosacea
    NARAYANAM RAO; Fiscal Year: 2009
    ..Most importantly, these sulfated and alkylated polysaccharides potently inhibit of the interaction of the receptor for advanced glycation end-products (RAGE) with its many ..
  64. STAPHYLOCOCCUS AUREUS: CAPSULAR ANTIGENS AND IMMUNITY
    Jean Lee; Fiscal Year: 1993
    ..Most S. aureus isolates are microencapsulated, i.e., they produce ureonic acid-containing extracellular polysaccharides that can be detected biochemically or serologically in bacterial cell extracts...
  65. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng Wang; Fiscal Year: 2009
    ..bacterial glycosylation system includes a highly promiscuous protein, PglL, which catalyzes the transfer of polysaccharides from a diphospho-lipid donor to target proteins...
  66. STRUCTURE OF THE RECEPTORS FOR ORAL BACTERIAL ADHESINS
    C Bush; Fiscal Year: 1991
    ..specific antibodies will be used to select representative strains of Streptococcus sanguis whose capsular polysaccharides are know to act as the receptors for the fimbrial lectins of Actinomyces species...
  67. Production of Polysaccharide-Protein Conjugates as Vaccines
    Wen Yi; Fiscal Year: 2009
    ..coli O157. Bacterial infections constitute one of the major health problems worldwide. Polysaccharides (or O-antigens), forming a thick capsule that surrounds the bacterial pathogen, represent a major determinant ..
  68. BACTEROIDES FRAGILIS CAPSULES--SYNTHESIS AND VIRULENCE
    Arthur Tzianabos; Fiscal Year: 1999
    ..abscess formation in the infected host and induces a form of T-cell immunity that is unusual for bacterial polysaccharides. The CPC comprises a complex of two discrete, surface expressed polysaccharides, termed polysaccharides A and ..
  69. POPULATION BIOLOGY OF SURFACE POLYSACCHARIDES IN CHOLERA
    John Morris; Fiscal Year: 2004
    ..of epidemic-associated 037 strains, there is increasing recognition that modifications in bacterial surface polysaccharides play a key role in the emergence of new, epidemic-associated strains...
  70. INNOVATIVE INTRAVESICAL TREATMENT APPROACH FOR IC
    Veer Bhavanandan; Fiscal Year: 2001
    ..are aimed at replacing the missing glycoconjugates by intravesical or oral administration of sulfated polysaccharides, such as heparin and pentosan polysulfate (Elmiron)...
  71. Molecular Principles of FGF-HSGAG Interactions
    Ram Sasisekharan; Fiscal Year: 2006
    ..The third major biopolymer is the polysaccharides. One of the most important groups of polysaccharides, in terms of its influence on important biological ..
  72. CATABOLISM OF POLYSACCHARIDES BY COLONIC BACTEROIDES
    Abigail Salyers; Fiscal Year: 2003
    ..One important activity of the colonic bacteria is the fermentation of dietary and host-derived polysaccharides. Fermentation products are absorbed by mucosal cells and used as a source of carbon and energy...
  73. Carboxylated polysaccharides for drug delivery to the eye
    Wendy Townsend; Fiscal Year: 2006
    ..Ocular Gel has the potential to improve both the effectiveness of ophthalmic drugs and increase patient compliance, for glaucoma as well as many other ocular conditions. [unreadable] [unreadable] [unreadable]..