mucins

Summary

Summary: High molecular weight mucoproteins that form a protective biofilm on the surface of EPITHELIAL CELLS where they provide a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.

Top Publications

  1. ncbi Muc2-deficient mice spontaneously develop colitis, indicating that MUC2 is critical for colonic protection
    Maria Van Der Sluis
    Division of Neonatology, Department of Pediatrics, Erasmus MC and Sophia Children s Hospital, Rotterdam, The Netherlands
    Gastroenterology 131:117-29. 2006
  2. pmc The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria
    Malin E V Johansson
    Department of Medical Biochemistry, University of Gothenburg, 405 30 Gothenburg, Sweden
    Proc Natl Acad Sci U S A 105:15064-9. 2008
  3. pmc Mucins in cancer: function, prognosis and therapy
    Donald W Kufe
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Cancer 9:874-85. 2009
  4. ncbi Mucins in cancer: protection and control of the cell surface
    Michael A Hollingsworth
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Nat Rev Cancer 4:45-60. 2004
  5. pmc Intestinal goblet cells and mucins in health and disease: recent insights and progress
    Young S Kim
    Genemed Biotechnologies, 458 Carlton Court, South San Francisco, CA 94080, USA
    Curr Gastroenterol Rep 12:319-30. 2010
  6. ncbi Colorectal cancer in mice genetically deficient in the mucin Muc2
    Anna Velcich
    Department of Oncology, Albert Einstein Cancer Center Montefiore Medical Center, 111 East 210 Street, Bronx, NY 10467, USA
    Science 295:1726-9. 2002
  7. ncbi Mucins in the mucosal barrier to infection
    S K Linden
    Mucosal Diseases Program, Mater Medical Research Institute and The University of Queensland, Level 3 Aubigny Place, Mater Hospitals, South Brisbane, Queensland, Australia
    Mucosal Immunol 1:183-97. 2008
  8. ncbi Composition and functional role of the mucus layers in the intestine
    Malin E V Johansson
    Department Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden
    Cell Mol Life Sci 68:3635-41. 2011
  9. ncbi Structure and function of the polymeric mucins in airways mucus
    David J Thornton
    Wellcome Trust Center for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Annu Rev Physiol 70:459-86. 2008
  10. pmc Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis
    Chad K Heazlewood
    Mucin and IBD Research Teams, Mucosal Diseases Program, Mater Medical Research Institute, and The University of Queensland, Aubigny Place, Mater Health Services, South Brisbane, Queensland, Australia
    PLoS Med 5:e54. 2008

Detail Information

Publications385 found, 100 shown here

  1. ncbi Muc2-deficient mice spontaneously develop colitis, indicating that MUC2 is critical for colonic protection
    Maria Van Der Sluis
    Division of Neonatology, Department of Pediatrics, Erasmus MC and Sophia Children s Hospital, Rotterdam, The Netherlands
    Gastroenterology 131:117-29. 2006
    ..Expression of mucin MUC2, the structural component of the colonic mucus layer, is lowered in inflammatory bowel disease. Our aim was to obtain insight in the role of Muc2 in epithelial protection...
  2. pmc The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria
    Malin E V Johansson
    Department of Medical Biochemistry, University of Gothenburg, 405 30 Gothenburg, Sweden
    Proc Natl Acad Sci U S A 105:15064-9. 2008
    ..These findings show that the Muc2 mucin can build a mucus barrier that separates bacteria from the colon epithelia and suggest that defects in this mucus can cause colon inflammation...
  3. pmc Mucins in cancer: function, prognosis and therapy
    Donald W Kufe
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Cancer 9:874-85. 2009
    Epithelia are protected from adverse conditions by a mucous barrier. The secreted and transmembrane mucins that constitute the mucous barrier are largely unrecognized as effectors of carcinogenesis...
  4. ncbi Mucins in cancer: protection and control of the cell surface
    Michael A Hollingsworth
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Nat Rev Cancer 4:45-60. 2004
  5. pmc Intestinal goblet cells and mucins in health and disease: recent insights and progress
    Young S Kim
    Genemed Biotechnologies, 458 Carlton Court, South San Francisco, CA 94080, USA
    Curr Gastroenterol Rep 12:319-30. 2010
    ..cells synthesize secretory mucin glycoproteins (MUC2) and bioactive molecules such as epithelial membrane-bound mucins (MUC1, MUC3, MUC17), trefoil factor peptides (TFF), resistin-like molecule beta (RELMbeta), and Fc-gamma binding ..
  6. ncbi Colorectal cancer in mice genetically deficient in the mucin Muc2
    Anna Velcich
    Department of Oncology, Albert Einstein Cancer Center Montefiore Medical Center, 111 East 210 Street, Bronx, NY 10467, USA
    Science 295:1726-9. 2002
    The gastrointestinal tract is lined by a layer of mucus comprised of highly glycosylated proteins called mucins. To evaluate the importance of mucin in intestinal carcinogenesis, we constructed mice genetically deficient in Muc2, the most ..
  7. ncbi Mucins in the mucosal barrier to infection
    S K Linden
    Mucosal Diseases Program, Mater Medical Research Institute and The University of Queensland, Level 3 Aubigny Place, Mater Hospitals, South Brisbane, Queensland, Australia
    Mucosal Immunol 1:183-97. 2008
    ..Mucin glycoproteins are secreted in large quantities by mucosal epithelia, and cell surface mucins are a prominent feature of the apical glycocalyx of all mucosal epithelia...
  8. ncbi Composition and functional role of the mucus layers in the intestine
    Malin E V Johansson
    Department Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden
    Cell Mol Life Sci 68:3635-41. 2011
    ..The progress in the last years in understanding the molecular nature of mucins, the main building blocks of mucus, has, however, changed this...
  9. ncbi Structure and function of the polymeric mucins in airways mucus
    David J Thornton
    Wellcome Trust Center for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Annu Rev Physiol 70:459-86. 2008
    ..In normal physiology, the secreted mucins, in particular the polymeric mucins MUC5AC and MUC5B, provide the organizing framework of the airways mucus gel ..
  10. pmc Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis
    Chad K Heazlewood
    Mucin and IBD Research Teams, Mucosal Diseases Program, Mater Medical Research Institute, and The University of Queensland, Aubigny Place, Mater Health Services, South Brisbane, Queensland, Australia
    PLoS Med 5:e54. 2008
    ..In this study we used random mutagenesis to produce two murine models of inflammatory bowel disease, characterised the basis and nature of the inflammation in these mice, and compared the pathology with human ulcerative colitis...
  11. ncbi Mucins, mucus, and sputum
    Judith A Voynow
    Department of Pediatrics, Duke University School of Medicine, Durham, NC 27710, USA
    Chest 135:505-12. 2009
    ..The mucins are composed of two major families: secreted mucins and membrane-associated mucins...
  12. ncbi Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites
    Karin Julenius
    Center for Biological Sequence Analysis, BioCentrum, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Glycobiology 15:153-64. 2005
    ..NetOGlyc 3.1 is made available at www.cbs.dtu.dk/services/netoglyc...
  13. ncbi Respiratory tract mucin genes and mucin glycoproteins in health and disease
    Mary Callaghan Rose
    Research Center for Genetic Medicine, Room 5700, Children s National Medical Center, 111 Michigan Avenue NW, Washington, DC 20010, USA
    Physiol Rev 86:245-78. 2006
    This review focuses on the role and regulation of mucin glycoproteins (mucins) in airway health and disease. Mucins are highly glycosylated macromolecules (> or =50% carbohydrate, wt/wt)...
  14. ncbi Mucins and mucin binding proteins in colorectal cancer
    James C Byrd
    The Department of Gastrointestinal Medicine and Nutrition, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Unit 436 Houston, TX 77030, USA
    Cancer Metastasis Rev 23:77-99. 2004
    b>Mucins are high-molecular weight epithelial glycoproteins with a high content of clustered oligosaccharides O-glycosidically linked to tandem repeat peptides rich in threonine, serine, and proline...
  15. pmc Mucin 13: structure, function, and potential roles in cancer pathogenesis
    Diane M Maher
    Cancer Biology Research Center, 2301 East 60th Street North, Sioux Falls, SD 57104, USA
    Mol Cancer Res 9:531-7. 2011
    ..MUC13 exhibits the characteristics suitable as an early marker for cancer screening and presents a promising target for antibody-guided targeted therapy...
  16. ncbi Trefoil factors: initiators of mucosal healing
    Douglas Taupin
    The Canberra Hospital, Canberra, Australian Capital Territory, Australia
    Nat Rev Mol Cell Biol 4:721-32. 2003
    ..TFFs protect mucous epithelia from a range of insults and contribute to mucosal repair, although the signalling events that mediate these responses are only partially understood...
  17. ncbi Mucolytic bacteria with increased prevalence in IBD mucosa augment in vitro utilization of mucin by other bacteria
    Chin Wen Png
    Mater Medical Research Institute and Department of Medicine, University of Queensland, Mater Health Services, South Brisbane, Queensland, Australia
    Am J Gastroenterol 105:2420-8. 2010
    ..We hypothesized that mucolytic bacteria are increased in IBD, providing increased substrate to sustain nonmucolytic mucosa-associated bacteria...
  18. pmc Intestinal trefoil factor confers colonic epithelial resistance to apoptosis
    D R Taupin
    Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, 55 Fruit Street, GRJ 719, Boston, MA 02114 2696, USA
    Proc Natl Acad Sci U S A 97:799-804. 2000
    ..These findings support a central role for ITF in the maintenance of intestinal mucosal continuity, and conversely demonstrate the potential for ITF expression to confer resistance of colorectal tumors to therapy...
  19. ncbi The VSL#3 probiotic formula induces mucin gene expression and secretion in colonic epithelial cells
    C Caballero-Franco
    Institute of Parasitology, McGill University, Saint Anne de Bellevue, Quebec, Canada
    Am J Physiol Gastrointest Liver Physiol 292:G315-22. 2007
    ..These findings contribute to a better understanding of the complex and beneficial interaction between colonic epithelial cells and intestinal bacteria...
  20. ncbi Structure and function of the cell surface (tethered) mucins
    Christine L Hattrup
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Mayo Clinic Arizona, Scottsdale, AR 85259, USA
    Annu Rev Physiol 70:431-57. 2008
    Cell surface mucins are large transmembrane glycoproteins involved in diverse functions ranging from shielding the airway epithelium against pathogenic infection to regulating cellular signaling and transcription...
  21. pmc Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM
    B Logan Buck
    Department of Food Science, North Carolina State University, Box 7624, Raleigh, NC 27695, USA
    Appl Environ Microbiol 71:8344-51. 2005
    ..This study demonstrated that multiple cell surface proteins in L. acidophilus NCFM can individually contribute to the organism's ability to attach to intestinal cells in vitro...
  22. pmc Gel-forming mucins appeared early in metazoan evolution
    Tiange Lang
    Department of Medical Biochemistry, Institute of Biomedicine, Goteborg University, Box 440, SE 405 30 Goteborg, Sweden
    Proc Natl Acad Sci U S A 104:16209-14. 2007
    b>Mucins are proteins that cover and protect epithelial cells and are characterized by domains rich in proline, threonine, and serine that are heavily glycosylated (PTS or mucin domains)...
  23. ncbi Mucins in human neoplasms: clinical pathology, gene expression and diagnostic application
    Suguru Yonezawa
    Department of Human Pathology, Field of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
    Pathol Int 61:697-716. 2011
    b>Mucins are high molecular weight glycoproteins that play important roles in carcinogenesis and tumor invasion...
  24. pmc Structural diversity and specific distribution of O-glycans in normal human mucins along the intestinal tract
    Catherine Robbe
    Unité Mixte de Recherche CNRS USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d Ascq cedex, France
    Biochem J 384:307-16. 2004
    Purified human mucins from different parts of the intestinal tract (ileum, cecum, transverse and sigmoid colon and rectum) were isolated from two individuals with blood group ALe(b) (A-Lewis(b))...
  25. pmc Mucin dynamics in intestinal bacterial infection
    Sara K Linden
    Mucosal Diseases Program, Mater Medical Research Institute, Mater Health Services, South Brisbane, Queensland, Australia
    PLoS ONE 3:e3952. 2008
    ..coli. Mucin glycoproteins are the main component of the first barrier that bacteria encounter in the intestinal tract...
  26. ncbi A new role for mucins in immunity: insights from gastrointestinal nematode infection
    Sumaira Z Hasnain
    Immunity, Infection and Inflammation Program, Mater Medical Research Institute, Mater Health Services and the University of Queensland, Brisbane, QLD 4029, Australia
    Int J Biochem Cell Biol 45:364-74. 2013
    ..The framework of this barrier is provided by polymeric, gel-forming mucins. These enormous O-linked glycoproteins are synthesised, stored and secreted by goblet cells that are also the ..
  27. ncbi Expression analysis of highly polymorphic mucin proteins (Sm PoMuc) from the parasite Schistosoma mansoni
    Emmanuel Roger
    Parasitologie Fonctionnelle et Evolutive, UMR 5244, CNRS Université de Perpignan, Perpignan Cedex, France
    Mol Biochem Parasitol 157:217-27. 2008
    ..Our data show that these proteins share structural characteristics with highly glycosylated secreted mucins. The proteins are (i) only expressed in larval stages that interact with the mollusc, (ii) located in the apical ..
  28. ncbi Mucins in inflammatory bowel diseases and colorectal cancer
    Yong H Sheng
    Immunity, Infection and Inflammation Program, Mater Medical Research Institute, South Brisbane, Queensland, Australia
    J Gastroenterol Hepatol 27:28-38. 2012
    The gastrointestinal tract is protected by a mucus barrier with both secreted and cell-surface mucins contributing to the exclusion of luminal microbes and toxins...
  29. ncbi Microbial modulation of innate defense: goblet cells and the intestinal mucus layer
    B Deplancke
    Department of Animal Sciences, University of Illinois at Urbana-Campaign, 61801, USA
    Am J Clin Nutr 73:1131S-1141S. 2001
    ..A precise definition of the regulatory networks that interface with goblet cells may have broad biomedical applications because mucus alterations appear to characterize most diseases of mucosal tissues...
  30. ncbi Inhibition of MUC4 expression suppresses pancreatic tumor cell growth and metastasis
    Ajay P Singh
    Department of Biochemistry and Molecular Biology, Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, USA
    Cancer Res 64:622-30. 2004
    ....
  31. ncbi Role of intestinal mucins in innate host defense mechanisms against pathogens
    Poonam Dharmani
    Gastrointestinal Research Group, Faculty of Medicine, University of Calgary, Calgary, Alta, Canada
    J Innate Immun 1:123-35. 2009
    Gastrointestinal mucins produced by goblet cells comprise the main structural components of the mucus layer...
  32. pmc Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel
    Martin E Lidell
    Department of Medical Biochemistry, Goteborg University, Medicinaregatan 9A, S 413 90 Gothenburg, Sweden
    Proc Natl Acad Sci U S A 103:9298-303. 2006
    ..Heavily O-glycosylated and protease-resistant mucin domains characterize gel-forming mucins. Their N- and C-terminal cysteine-rich domains are involved in mucin polymerization, and these domains are likely ..
  33. ncbi Trypanosoma cruzi surface mucins: host-dependent coat diversity
    Carlos A Buscaglia
    Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus IIB INTECH, Universidad Nacional de General San Martn CONICET, San Martin 1650, Buenos Aires, Argentina
    Nat Rev Microbiol 4:229-36. 2006
    The surface of the protozoan parasite Trypanosoma cruzi is covered in mucins, which contribute to parasite protection and to the establishment of a persistent infection...
  34. pmc Mucins and inflammatory bowel disease
    T Shirazi
    University of Bristol, Bristol Royal Infirmary University Department of Medicine, UK
    Postgrad Med J 76:473-8. 2000
    ..The mucins that make up this layer consist of a protein backbone with oligosaccharides attached to specific areas of the ..
  35. ncbi Interleukin-13 induces goblet cell differentiation in primary cell culture from Guinea pig tracheal epithelium
    Mitsuko Kondo
    First Department of Medicine, Tokyo Women s Medical University, School of Medicine, Japan
    Am J Respir Cell Mol Biol 27:536-41. 2002
    ..These data suggest that human recombinant IL-13, but not IL-4, can induce differentiation into mature goblet cells that produce MUC5AC protein in guinea pig tracheal epithelial cells in vitro...
  36. ncbi Immunomodulation of monocyte-derived dendritic cells through ligation of tumor-produced mucins to Siglec-9
    Mariko Ohta
    Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kita ku, Kyoto, Japan
    Biochem Biophys Res Commun 402:663-9. 2010
    ..In the present study, we investigated whether or not the ligation of tumor-produced mucins with Siglec-9 expressed on immature DCs is related to escape from immunosurveillance in the tumor-bearing state...
  37. ncbi New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7
    K Rousseau
    Galton Laboratory, Department of Biology, University College London, United Kingdom
    Hybrid Hybridomics 22:293-9. 2003
    The separation and characterization of salivary mucins is not straightforward because of their large size, heterogeneity, and molecular interactions...
  38. pmc Mucins in the pathogenesis of breast cancer: implications in diagnosis, prognosis and therapy
    Partha Mukhopadhyay
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Biochim Biophys Acta 1815:224-40. 2011
    b>Mucins are high molecular weight, multifunctional glycoproteins comprised of two structural classes-the large transmembrane mucins and the gel-forming or secreted mucins...
  39. ncbi Trans-sialidase and mucins of Trypanosoma cruzi: an important interplay for the parasite
    M Eugenia Giorgi
    CIHIDECAR, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
    Carbohydr Res 346:1389-93. 2011
    ..a unique enzyme, the trans-sialidase (TcTS) involved in the transfer of sialic acid from host glycoconjugates to mucins of the parasite...
  40. ncbi Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4
    Jean Luc Desseyn
    Unite 560 INSERM, Place de Verdun, 59045 Lille Cedex, France
    Eur J Biochem 269:3150-9. 2002
    ..Based on these data, we concluded that we isolated the mouse ortholog of MUC4 we propose as Muc4. Expression studies showed that Muc4 is ubiquitous like SMC and MUC4, with highest levels of expression in trachea and intestinal tract...
  41. pmc Carcinoma mucins trigger reciprocal activation of platelets and neutrophils in a murine model of Trousseau syndrome
    Bojing Shao
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    Blood 118:4015-23. 2011
    ..Injecting carcinoma mucins into mice generates platelet-rich microthrombi dependent on P- and L-selectin but not thrombin...
  42. pmc Gastric MUC5AC and MUC6 are large oligomeric mucins that differ in size, glycosylation and tissue distribution
    Henrik Nordman
    Mucosal Biology Group, Department of Cell and Molecular Biology, Level C13, BMC Lund University, S 221 84, Lund, Sweden
    Biochem J 364:191-200. 2002
    Gastric MUC5AC and MUC6 mucins were studied using polyclonal antibodies. Immunohistochemistry showed MUC5AC to originate from the surface epithelium, whereas MUC6 was produced by the glands...
  43. ncbi Human mucin gene MUC5B, the 10.7-kb large central exon encodes various alternate subdomains resulting in a super-repeat. Structural evidence for a 11p15.5 gene family
    J L Desseyn
    INSERM 377 Laboratoire Gérard Biserte, Place de Verdun, 59045 Lille Cedex, France
    J Biol Chem 272:3168-78. 1997
    ..The super-repeat present in MUC5B is the largest ever determined in mucin genes and the central exon of this gene is, by far, the largest reported for a vertebrate gene...
  44. pmc Central role of Muc5ac expression in mucous metaplasia and its regulation by conserved 5' elements
    Hays W J Young
    Department of Pulmonary Medicine, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Am J Respir Cell Mol Biol 37:273-90. 2007
    Mucus hypersecretion contributes to morbidity and mortality in many obstructive lung diseases. Gel-forming mucins are the chief glycoprotein components of airway mucus, and elevated expression of these during mucous metaplasia precedes ..
  45. pmc Extracellular MUC3 mucin secretion follows adherence of Lactobacillus strains to intestinal epithelial cells in vitro
    D R Mack
    Department of Pediatrics, University of Ottawa, Canada
    Gut 52:827-33. 2003
    b>Mucins are large complex glycoproteins that protect intestinal mucosal surfaces by limiting access of environmental matter to their epithelial cells...
  46. pmc Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier
    PABLO ARGUESO
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA
    J Biol Chem 284:23037-45. 2009
    ..of defense has been the subject of much investigation, and there is evidence now implicating O-glycosylated mucins on the epithelial cell surface...
  47. pmc Tumor necrosis factor alpha-converting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells
    Matt X G Shao
    Cardiovascular Research Institute and Departments of Medicine and Physiology, University of California, San Francisco, CA 94143 0130, USA
    Proc Natl Acad Sci U S A 100:11618-23. 2003
    ..From these results, we conclude that TACE plays a critical role in mucin production by airway epithelial cells by means of a TACE ligand-EGFR cascade in response to various stimuli...
  48. ncbi Comparison of in vitro models to study bacterial adhesion to the intestinal epithelium
    J M Laparra
    Institute of Agrochemistry and Food Technology, Burjassot, Valencia, Spain
    Lett Appl Microbiol 49:695-701. 2009
    ..To evaluate the adhesion ability of intestinal bacteria to different in vitro models of intestinal epithelia, and to estimate the suitability of these models and the type of interactions involved...
  49. pmc Current status of mucins in the diagnosis and therapy of cancer
    Satyanarayana Rachagani
    Department of Biochemistry and Molecular Biology, Department of Pathology and Microbiology, Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 5870, USA
    Biofactors 35:509-27. 2009
    b>Mucins are the most abundant high molecular weight glycoproteins in mucus...
  50. pmc Fusobacterium nucleatum infection of colonic cells stimulates MUC2 mucin and tumor necrosis factor alpha
    Poonam Dharmani
    Gastrointestinal Research Group, Health Sciences Centre, University of Calgary, 3330 Hospital Drive, NW, Calgary, Alberta T2N 4N1, Canada
    Infect Immun 79:2597-607. 2011
    ..nucleatum may represent a challenging pathogen in the etiology of gut inflammatory diseases and highlight the importance of different pathotypes of candidate bacterial species in disease pathogenesis...
  51. pmc The genome of Akkermansia muciniphila, a dedicated intestinal mucin degrader, and its use in exploring intestinal metagenomes
    Mark W J van Passel
    Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
    PLoS ONE 6:e16876. 2011
    ..This anaerobic resident is associated with the protective mucus lining of the intestines...
  52. ncbi Clinical potential of mucins in diagnosis, prognosis, and therapy of ovarian cancer
    Ajay P Singh
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA
    Lancet Oncol 9:1076-85. 2008
    Knowledge of mucins and their multiple roles in various normal and pathological processes has improved greatly in the past two decades...
  53. ncbi The N terminus of the MUC2 mucin forms trimers that are held together within a trypsin-resistant core fragment
    Klaus Godl
    Department of Medical Biochemistry, Goteborg University, Gothenburg, Sweden
    J Biol Chem 277:47248-56. 2002
    ....
  54. ncbi Biological significance of cancer-associated sialyl-Tn antigen: modulation of malignant phenotype in gastric carcinoma cells
    Sandra Pinho
    Institute of Molecular Pathology and Immunology of the University of Porto IPATIMUP, Rua Dr Roberto Frias, s n 4200 465 Porto, Portugal
    Cancer Lett 249:157-70. 2007
    ..Sialyl-Tn antigen is able to modulate a malignant phenotype inducing a more aggressive cell behavior, such as decreased cell-cell aggregation and increased ECM adhesion, migration and invasion...
  55. pmc MUC4 mucin interacts with and stabilizes the HER2 oncoprotein in human pancreatic cancer cells
    Pallavi Chaturvedi
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 5870, USA
    Cancer Res 68:2065-70. 2008
    ..Our findings add a new dimension to MUC4 function as a modulator of cell signaling and provide mechanistic evidence for its role in pancreatic cancer progression...
  56. ncbi Architecture of the large membrane-bound mucins
    Jean Luc Desseyn
    INSERM U837 JPARC, 59045 Lille, France
    Gene 410:215-22. 2008
    Epithelial membrane-bound mucins are high molecular mass glycoproteins that may be also secreted or released into the extracellular environment...
  57. ncbi MUC5AC and MUC5B mucins increase in cystic fibrosis airway secretions during pulmonary exacerbation
    Markus O Henke
    Philipps University Marburg, Department of Pulmonary Medicine, Baldingerstrasse 1, 35043 Marburg, Germany
    Am J Respir Crit Care Med 175:816-21. 2007
    Cystic fibrosis (CF) is believed to be associated with mucus hypersecretion; thus, the principal airway gel-forming mucins, MUC5AC and MUC5B, are also expected to be increased relative to non-CF secretions...
  58. ncbi The membrane-bound mucins: From cell signalling to transcriptional regulation and expression in epithelial cancers
    Nicolas Jonckheere
    INSERM, Jean Pierre Aubert Research Centre, Lille, France
    Biochimie 92:1-11. 2010
    The membrane-bound mucins belong to an ever-increasing family of O-glycoproteins...
  59. ncbi MUC1, MUC2, MUC5AC, and MUC6 expressions in gastric carcinomas: their roles as prognostic indicators
    H S Lee
    Department of Pathology, Seoul National University College of Medicine, Seoul, Korea
    Cancer 92:1427-34. 2001
    ..between clinicopathologic profiles and the patients' survival, the expression of MUC1, MUC2, MUC5AC, MUC6 mucins and the p53 protein were evaluated immunohistochemically in 300 consecutive gastric carcinomas using the tissue-..
  60. ncbi Pathogenesis of carcinoma of the papilla of Vater
    Hans Peter Fischer
    Department of Pathology, University of Bonn, Sigmund Freud Str 25, D 53127, Bonn, Germany
    J Hepatobiliary Pancreat Surg 11:301-9. 2004
    ..Consequently, the histologic classification should reflect the histogenesis of ampullary tumors from the two different types of papillary mucosa...
  61. ncbi Diagnostic ions for the rapid analysis by nano-electrospray ionization quadrupole time-of-flight mass spectrometry of O-glycans from human mucins
    Catherine Robbe
    Unité Mixte de Recherche CNRS USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d Ascq cedex, France
    Rapid Commun Mass Spectrom 18:412-20. 2004
    ..was used for sensitive mapping and sequencing of underivatized oligosaccharide alditols obtained from human mucins. Using subnanomolar amounts of oligosaccharides previously analyzed by nuclear magnetic resonance (NMR), series of ..
  62. ncbi The murine mCLCA3 (alias gob-5) protein is located in the mucin granule membranes of intestinal, respiratory, and uterine goblet cells
    Ina Leverkoehne
    Department of Pathology, School of Veterinary Medicine Hannover, Hannover, Germany
    J Histochem Cytochem 50:829-38. 2002
    ..The results suggest that mCLCA3 may be involved in the synthesis, condensation, or secretion of mucins.
  63. ncbi Inhibition of Escherichia coli O157:H7 attachment by interactions between lactic acid bacteria and intestinal epithelial cells
    Younghoon Kim
    Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju, Korea
    J Microbiol Biotechnol 18:1278-85. 2008
    ..Intestinal tract mucins are principal components of the mucus layer on epithelial surfaces, and perform a protective function against ..
  64. pmc Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model
    Pieter Van den Abbeele
    Laboratory of Microbial Ecology and Technology LabMET, Ghent University, Ghent, Belgium
    ISME J 7:949-61. 2013
    ..butyrate producers within this cluster, Roseburia intestinalis and Eubacterium rectale most specifically colonized mucins. These 16S rRNA gene-based results were confirmed at a functional level as butyryl-CoA:acetate-CoA transferase ..
  65. ncbi The Trypanosoma cruzi membrane mucin AgC10 inhibits T cell activation and IL-2 transcription through L-selectin
    Pilar Alcaide
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Madrid, 28049 Spain
    Int Immunol 16:1365-75. 2004
    ..Our results suggest that Ag C10, through binding to L-selectin, was able to inhibit different activation pathways that lead to inhibition of IL-2 secretion and T cell proliferation. This was independent of NO and IFN-gamma...
  66. ncbi Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor
    K Kohri
    Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94143 0130, USA
    Eur Respir J 20:1263-70. 2002
    ..The authors conclude that PA products induce mucin MUC5AC production in human airway epithelial cells via the expression and activation of epidermal growth factor receptor...
  67. ncbi O-glycosylation of the mucin type
    F G Hanisch
    Institute of Biochemistry, Medical Faculty of the University, , Germany
    Biol Chem 382:143-9. 2001
    ....
  68. pmc Structure and biological roles of mucin-type O-glycans at the ocular surface
    Ana Guzman-Aranguez
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA
    Ocul Surf 8:8-17. 2010
    b>Mucins are major components in mucus secretions and apical cell membranes on wet-surfaced epithelia...
  69. ncbi Cardioprotective proteins upregulated in the liver in response to experimental myocardial ischemia
    Shu Q Liu
    Biomedical Engineering Department, Northwestern University, Evanston, Illinois 60208 3107, USA
    Am J Physiol Heart Circ Physiol 303:H1446-58. 2012
    ..These observations suggest that the liver contributes to cardioprotection in MI by upregulating and releasing protective secretory proteins. These proteins may be used for the development of cardioprotective agents...
  70. pmc Mucin production during prenatal and postnatal murine lung development
    Michelle G Roy
    Department of Pulmonary Medicine, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Am J Respir Cell Mol Biol 44:755-60. 2011
    ..To identify potential roles for secreted gel--forming mucins in lung development, we isolated murine lungs on embryonic days (E) 12.5-18...
  71. ncbi Exploring the transcriptome of Atlantic salmon (Salmo salar) skin, a major defense organ
    Giulia Micallef
    Institute of Biological and Environmental Sciences, University of Aberdeen, Tillydrone Avenue, Aberdeen, UK
    Mar Biotechnol (NY) 14:559-69. 2012
    ..Several isotigs exhibited homology to mammalian mucins (MUC2, MUC5AC and MUC5B)...
  72. pmc The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins
    Angelina S Palma
    Glycosciences Laboratory, Department of Medicine, Imperial College London, Northwick Park and St Marks Campus, Watford Road, Harrow, Middlesex, UK
    Biochem Biophys Res Commun 408:548-52. 2011
    ....
  73. ncbi Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment
    Joan Vermeiren
    Laboratory of Microbial Ecology and Technology, Ghent University, Gent, Belgium
    FEMS Microbiol Ecol 79:685-96. 2012
    ..The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV...
  74. ncbi Expression of MUC5AC in colorectal carcinoma and relationship with prognosis
    Belma Kocer
    Department of Second General Surgery, Ankara Numune Teaching and Research Hospital, Ankara, Turkey
    Pathol Int 52:470-7. 2002
    Overexpression and alterations in the glycosylation of gastric mucins have been described in colorectal carcinoma...
  75. pmc Recent insights into the biological roles of mucin-type O-glycosylation
    E Tian
    Developmental Glycobiology Unit, NIDCR, National Institutes of Health, Building 30, Room 426, 30 Convent Drive, MSC 4370, Bethesda, MD 20892 4370, USA
    Glycoconj J 26:325-34. 2009
    ..Gaining an understanding of mucin-type O-glycans in these diverse systems will elucidate crucial conserved processes underlying many aspects of development and homeostasis...
  76. pmc Intraspecies variation in Trypanosoma cruzi GPI-mucins: biological activities and differential expression of α-galactosyl residues
    Rodrigo P Soares
    Centro de Pesquisas Rene Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil
    Am J Trop Med Hyg 87:87-96. 2012
    The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T...
  77. pmc Accelerated progression of gastritis to dysplasia in the pyloric antrum of TFF2 -/- C57BL6 x Sv129 Helicobacter pylori-infected mice
    James G Fox
    Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Am J Pathol 171:1520-8. 2007
    ..pylori-infected mice at 19 months pi. These data support a protective role for TFF2 in part by modulating levels of gastric interferon-gamma in the development of H. pylori-associated premalignancy of the distal stomach...
  78. pmc A morphometric study of mucins and small airway plugging in cystic fibrosis
    Pierre Regis Burgel
    Service de Pneumologie, Hopital Cochin, Universite Rene Descartes, 27 rue du Faubourg St Jacques, 75 679 Paris Cedex 14, France
    Thorax 62:153-61. 2007
    ..Little knowledge exists on structural changes and plugging in small airways in cystic fibrosis...
  79. ncbi IFN-gamma-induced expression of MUC4 in pancreatic cancer cells is mediated by STAT-1 upregulation: a novel mechanism for IFN-gamma response
    M Andrianifahanana
    Department of Biochemistry and Molecular Biology, The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA
    Oncogene 26:7251-61. 2007
    ..In conclusion, our findings identify a novel mechanism for MUC4 regulation in pancreatic cancer cells and unfold new perspectives on the foundation of IFN-gamma-dependent gene regulation...
  80. ncbi Structural organization and classification of the human mucin genes
    N Moniaux
    Unite 377 INSERM, Place de Verdun, 59045 Lille Cedex and Laboratoire de Biochimie et de Biologie Moleculaire de l'Hopital C. Huriez, 59037 Lille Cedex, France
    Front Biosci 6:D1192-206. 2001
    ..With evolution, defense mechanisms, such as the secretion of mucus has been developed. Mucins are the main components of mucus...
  81. pmc TFF2/SP-deficient mice show decreased gastric proliferation, increased acid secretion, and increased susceptibility to NSAID injury
    James J Farrell
    Massachusetts General Hospital, Gastrointestinal Unit, Boston, Massachusetts, USA
    J Clin Invest 109:193-204. 2002
    ..Taken together, these results suggest a physiologic role for TFF2 to promote mucosal healing through the stimulation of proliferation and downregulation of gastric acid secretion...
  82. ncbi Epidermal growth factor receptor-mediated innate immune responses and their roles in airway diseases
    P R Burgel
    Service de Pneumologie and UPRES EA 2511, Universite Paris Descartes, Hopital Cochin, Assistance Publique Hopitaux de Paris, Paris, France
    Eur Respir J 32:1068-81. 2008
    ..In considering each therapeutic intervention, the potential benefits must be considered in relation to potential deleterious effects...
  83. pmc Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast
    Nadia Vadaie
    Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA
    J Cell Biol 181:1073-81. 2008
    Signaling mucins are cell adhesion molecules that activate RAS/RHO guanosine triphosphatases and their effector mitogen-activated protein kinase (MAPK) pathways...
  84. ncbi Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose
    E Gaudier
    Human Nutrition and Gut Function Department, Institut National de la Recherche Agronomique, Nantes, France
    Am J Physiol Gastrointest Liver Physiol 287:G1168-74. 2004
    ..It is mainly composed of high molecular weight glycoproteins called mucins. Butyrate, a short-chain fatty acid produced during carbohydrate fermentation, has been shown to increase mucin ..
  85. ncbi Interleukin-1beta induces MUC2 and MUC5AC synthesis through cyclooxygenase-2 in NCI-H292 cells
    Yong Dae Kim
    Department of Otorhinolaryngology, College of Medicine, Yeungnam University, Nam Gu, Daegu, South Korea
    Mol Pharmacol 62:1112-8. 2002
    ..These results indicate that in human pulmonary epithelial cells, IL-1beta activates ERK or p38 to induce COX-2 production, which in turn induces MUC2 and MUC5AC production...
  86. pmc MUC1 and MUC2 in pancreatic neoplasia
    E Levi
    John Dingell Veterans Administration Medical Center, 4646 John Road, Detroit, MI 48201, USA
    J Clin Pathol 57:456-62. 2004
    ..Thus, in pancreatic neoplasia, MUC1 and MUC2 have potential diagnostic and prognostic value as markers of aggressive and indolent phenotypes, respectively, and have potential as therapeutic targets...
  87. ncbi Muc4/sialomucin complex, the intramembrane ErbB2 ligand, in cancer and epithelia: to protect and to survive
    Kermit L Carraway
    Department of Cell Biology and Anatomy, University of Miami School of Medicine, Florida 33101, USA
    Prog Nucleic Acid Res Mol Biol 71:149-85. 2002
    ..The soluble form facilitates the protection and lubrication of epithelia by mucous gels composed of gel-forming mucins, as in the airway, where Muc4 is proposed to participate in mucociliary transport as a constituent of the ..
  88. ncbi Structure and biosynthesis of human salivary mucins
    A Zalewska
    Department of Pharmaceutical Biochemistry Institute of Chemistry, Białystok, Poland
    Acta Biochim Pol 47:1067-79. 2000
    Human salivary glands secrete two types of mucins: oligomeric mucin (MG1) with molecular mass above 1 MDa and monomeric mucin (MG2) with molecular mass of 200-250 kDa...
  89. ncbi Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor
    J R Gum
    Gastrointestinal Research Laboratory 151M2, Department of Veterans Affairs Medical Center, San Francisco, California
    J Biol Chem 269:2440-6. 1994
    Secretory mucins consist of a protein backbone that is catenated by disulfide bonds, heavily O-glycosylated, and packaged into storage granules prior to release from cells...
  90. ncbi The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region
    J R Gum
    Gastrointestinal Research Laboratory, Department of Veterans Affairs Medical Center, San Francisco, California
    J Biol Chem 267:21375-83. 1992
    ..This latter subdomain exhibits varying degrees of sequence similarity to a wide range of mucins and mucin-like proteins including those isolated from rats, pigs, cows, and frogs...
  91. pmc The StcE protease contributes to intimate adherence of enterohemorrhagic Escherichia coli O157:H7 to host cells
    Thomas E Grys
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, 1300 University Ave, Room 481 MSC, Madison, WI 53706, USA
    Infect Immun 73:1295-303. 2005
    ..Thus, StcE may help block host clearance of E. coli O157:H7 by destruction of some classes of glycoproteins, and it contributes to intimate adherence of E. coli O157:H7 to the HEp-2 cell surface...
  92. pmc Two atypical enteropathogenic Escherichia coli strains induce the production of secreted and membrane-bound mucins to benefit their own growth at the apical surface of human mucin-secreting intestinal HT29-MTX cells
    Monica A M Vieira
    INSERM, UMR756 Signalisation et Physiopathologie des Cellules Epithéliales, Chatenay Malabry Cedex, France
    Infect Immun 78:927-38. 2010
    ..we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins...
  93. ncbi Hath1 up-regulates gastric mucin gene expression in gastric cells
    Akiko Sekine
    Department of Molecular Oncology, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1 5 45, Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Biochem Biophys Res Commun 344:1166-71. 2006
    ..These data indicate that Hath1 is one of the transcriptional regulators for MUC6 and MUC5AC in GC cells. It is also possible that loss of Hath1 expression may play a role in gastric carcinogenesis...
  94. ncbi Intestinal barrier failure during experimental necrotizing enterocolitis: protective effect of EGF treatment
    Jessica A Clark
    Department of Pediatrics, University of Arizona, Tucson, AZ 85724 5073, USA
    Am J Physiol Gastrointest Liver Physiol 291:G938-49. 2006
    ..EGF treatment maintains intestinal integrity at the site of injury by accelerating goblet cell maturation and mucin production and normalizing expression of TJ proteins, leading to improved intestinal barrier function...
  95. pmc Human MUC1 carcinoma-associated protein confers resistance to genotoxic anticancer agents
    Jian Ren
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 5:163-75. 2004
    ..These findings indicate that MUC1 attenuates the apoptotic response to DNA damage and that this oncoprotein confers resistance to genotoxic anticancer agents...
  96. pmc Glycosylation variants of mucins and CEACAMs as candidate biomarkers for the diagnosis of pancreatic cystic neoplasms
    Brian B Haab
    Van Andel Research Institute, Grand Rapids, MI, USA
    Ann Surg 251:937-45. 2010
    ..Here we explored whether glycosylation variants on specific proteins in cyst fluid samples could serve as biomarkers to aid in this diagnosis...
  97. ncbi Trefoil peptides: from structure to function
    L Thim
    Department of Protein Chemistry, Novo Nordisk A S, Bagsvaerd, Denmark
    Cell Mol Life Sci 53:888-903. 1997
    ..This review summarises our present knowledge of trefoil peptide structures, their sites of expression, and their protection and repair functions, with a focus on the mechanism by which these peptides exert their biological function...
  98. ncbi Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs
    N Porchet
    Unité 16 INSERM, Lille, France
    Biochem Biophys Res Commun 175:414-22. 1991
    ..with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-..
  99. pmc Colitis-associated variant of TLR2 causes impaired mucosal repair because of TFF3 deficiency
    Daniel K Podolsky
    Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas, USA
    Gastroenterology 137:209-20. 2009
    ..The aim of this study was to determine whether Toll-like receptor (TLR) 2 and modulation of GC-derived trefoil factor (TFF) 3 are functionally linked in the intestine...
  100. ncbi Generation and characterization of anti-MUC4 monoclonal antibodies reactive with normal and cancer cells in humans
    Nicolas Moniaux
    Department of Biochemistry and Molecular Biology and Eppley Institute for Research in Cancer and Allied Disease, University of Nebraska Medical Center, Omaha, Nebraska 68198 4525, USA
    J Histochem Cytochem 52:253-61. 2004
    ..Anti-MUC4 MAb may represent a powerful tool for the study of MUC4 function under normal and pathological conditions and for diagnosis of solid tumors including those in the breast, pancreas, lungs, and ovaries...
  101. ncbi The MUC2 gene product: a human intestinal mucin
    A Allen
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, UK
    Int J Biochem Cell Biol 30:797-801. 1998
    ..The MUC2 product is polymerised end to end through disulphide bridges to form large secreted polymeric gel-forming mucins (Mr approximately 10(7))...

Research Grants71

  1. BIOSYNTHESIS OF TRACHEAL MUCOUS GLYCOPROTEINS
    Pi Wan Cheng; Fiscal Year: 2010
    ..Secreted from goblet cells on the surface epithelium and mucus cells in the submucosal glands, mucins not only are the major determinant of the viscoelastic properties of mucus secretion but also can serve as the ..
  2. Nanoparticle Coupled Antioxidants for Respiratory Illness in Veterans
    RODNEY JON SCHLOSSER; Fiscal Year: 2013
    ..We will use a murine model of allergic fungal rhinosinusitis with exacerbation by smoke or DEP exposure to determine the efficacy of antiMUC1-SOD/CAT-NPs. ..
  3. Regulation of Airway Mucin Gene Expression by Epigenetic Mechanism
    Reen Wu; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Mucins are major contributors to the visco-elastic properties of mucus secretion, which plays an important role in the mucociliary clearance in conducting airways...
  4. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013
    ..The ion transport properties of the upper and lower airways wil be determined, as these are reflective of the hydration status of the ASL. Tissue samples from mice 3, 6, 12, and 24 months old will be studied in Ussing chambers. ..
  5. ROLE OF MUC1 IN THE GENESIS OF ALLERGIC ASTHMA
    Kwang Chul Kim; Fiscal Year: 2010
    ..In this project, we will try to understand the mechanism at the cellular and molecular level. Successful completion of this project will shed valuable insights in our understanding of the development of asthma. ..
  6. Mouse models for esophageal Cox-2 oxidative stress and DNA damage
    John P Lynch; Fiscal Year: 2013
    ....
  7. Counter-Regulation of TLR-Driven Mucosal Inflammation
    Erik P Lillehoj; Fiscal Year: 2010
    ..Successful accomplishment of this project will provide pharmacological strategies to control excessive inflammation during airway bacterial colonization of mucosal surfaces. ..
  8. Mechanisms of oxidant-induced chronic bronchitis
    ROSANNA C MALBRAN FORTEZA; Fiscal Year: 2012
    ....
  9. Nanotheranostics for Early Colorectal Cancer Detection and Treatment
    Jeffrey S Souris; Fiscal Year: 2013
    ..with lectins/aptamers/peptides that target M-T stage-dependent, aberrant polyp glycan/glycoprotein expression (mucins MUC1 and MUC15, mucin-like tumor-associated glycoprotein TAG-72, and mucin surface glycan [unreadable]-L-fucose)...
  10. The Role of Beta-Gal Mediated Interactions in Prostate Cancer Metastasis
    VLADISLAV V GLINSKII; Fiscal Year: 2013
    ..abstract_text> ..
  11. Modeling oxidative stress and DNA damage using GI organotypic culture systems
    John P Lynch; Fiscal Year: 2013
    ..It will also foster the development of novel therapeutic and preventive strategies that will improve patient care for these important clinical conditions. ..
  12. H. influenzae: Role of Sialic Acid in Virulence
    Michael A Apicella; Fiscal Year: 2013
    ..Finally, studies of isolates from human subjects colonized with NTHi 2019strR will examine the role of phase variation on LOS structure and determine if foreign DNA is acquired by NTHi during colonization. ..
  13. Oral Mucins as a Diagnostic Indicator and Therapeutic Treatment for Xerostomia
    DAVID JOHN CULP; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Mucins are a primary component of mucus covering those parts of the body exposed to the environment, including the airways, digestive tract, urogenital system, and the oral cavity...
  14. Probiotics and HIF signaling in Alcoholic Liver Disease
    Wenke Feng; Fiscal Year: 2013
    ..Protection of the mucus layer involves mucins and a number of regulatory peptides, such as intestinal trefoil factors (ITFs), which play critical rols in ..
  15. IL8-induced Post-transcriptional Regulation of the MUC5AC mucin gene
    Mary C Rose; Fiscal Year: 2010
    ..b>Mucins are hypersecreted and overproduced in lung diseases, thereby contributing to airway mucus obstruction and to ..
  16. H. influenzae: Role of Sialic Acid in Virulence
    Michael Apicella; Fiscal Year: 2009
    ..abstract_text> ..
  17. Effect of Helicobacter pylori cell shape on pathogenesis
    LAURA ELIZABETH MARTINEZ; Fiscal Year: 2013
    ..We will also investigate the mechanisms by which PG recycling mutants cause elevated pro-inflammatory cytokine release (aim 3B). ..
  18. Alcohol and Breast Cancer
    Jia Luo; Fiscal Year: 2012
    ..Understanding the interactions among alcohol, ErbB2 and MUC1 will also provide an important insight into the pathogenesis of some human diseases related to alcohol abuse as well as alcohol's teratogenic effect during development. ..
  19. Sulfotransferases in the Synthesis of L-Selectin Ligands
    Steven D Rosen; Fiscal Year: 2010
    ..To evaluate this hypothesis, we will determine whether isolated airway mucins can activate neutrophils through binding to L-selectin and cause the secretion of broncho-active substances...
  20. Bile Acids, Genetic Control and Colonic Function in Irritable Bowel Syndrome
    MICHAEL L CAMILLERI; Fiscal Year: 2013
    ....
  21. Role of mucin-type O-glycosylation in Trypanosoma cruzi biology
    Christopher M West; Fiscal Year: 2012
    ..of mucin-type glycoproteins include the sugar 1GlcNAc forming the bridge between O-glycans and Thr residues of the mucins, 2Gal residues found both in the pyranosidic and/or furanosidic forms, and terminal 13Gal or sialic-acid residues, ..
  22. Development of Therapeutic Pan-Filovirus Macaque Monoclonal Antibodies
    Sven G Enterlein; Fiscal Year: 2013
    ..Upon successful completion of this Phase I SBIR we envision a Phase II focused on demonstration of protective efficacy in NHP models and standard pre-clinical development activities in preparation for regulatory filing. ..
  23. Glycosyltransferase Golgi Retention Mechanism
    Pi Wan Cheng; Fiscal Year: 2013
    ..glycoproteins and C2GnT-M synthesizes all three branch structures, core 2, core 4, and I antigen, in secreted mucins while the function of C2GnT-3 is unknown...
  24. Oligosaccharide moieties of human milk glycans that inhibit pathogens
    David S Newburg; Fiscal Year: 2013
    ..Glycans, including glycoproteins, glycolipids, mucins, and glycosaminoglycans, contain complex oligosaccharide structures attached to proteins, lipids, and other ..
  25. Development of an Anti-PSGL-1 Antibody to Treat Crohn's Disease
    Scott A Rollins; Fiscal Year: 2011
    ..S. The drug being developed addresses a major unmet medical need for a therapy that can maintain remission and/or ameliorate the devastating effects of this disease. ..
  26. Oxyntic Atrophy and Novel Gastric Lineages
    JAMES RICHARD GOLDENRING; Fiscal Year: 2013
    ..mucosa is assembled from a heterogeneous group of epithelial lineages responsible for the normal secretion of mucins, pepsinogen and HCl. Chronic injury associated with H...
  27. Impact of Inflammatory Genes on Glandular Hyperplasia in Children with Sinusitis
    Mary C Rose; Fiscal Year: 2010
    ....
  28. CYTOKINE INFLAMMATORY MEDIATORS AND MUCIN REGULATION IN MIDDLE EAR EPITHELIUM
    Joseph E Kerschner; Fiscal Year: 2013
    ..Specifically, gel-forming mucins (GFM), produced by middle ear mucosa (MEM), are known to be the primary cause of hearing loss which develops in ..
  29. Trafficking mechanisms for secretory vesicles in pancreatic duct epithelial cells
    Duk Su Koh; Fiscal Year: 2013
    ..The experiments are broadly relevant to treatment of disorders of the digestive system. ..
  30. Control of Mucin Glycan Branching in Membrane-bound and Secreted Mucins
    Pi Wan Cheng; Fiscal Year: 2010
    ..They are found primarily in secreted mucins and membrane-bound glycoproteins, including some mucins...
  31. Airway Epithelial Responses to Allergic Inflammation
    David J Erle; Fiscal Year: 2010
    ..Asthma is a major public health problem that affects tens of millions of Americans and this work will help to identify and assess several potential new targets for asthma treatment. ..
  32. TIM Family of Genes: Role in T Cell Immunity and Tolerance
    Vijay K Kuchroo; Fiscal Year: 2012
    ..abstract_text> ..
  33. Regulation of Vibrio cholerae motility, stress response and detachment from intes
    ANISIA J SILVA-BENITEZ; Fiscal Year: 2013
    ..As an in vitro model we will use HT29-18N2 cultured cells which can be induced to differentiate into mucin-secreting goblet cells. As an in vivo model we will use adult rabbit ileal loops. ..
  34. Development of a novel second-generation pan-filovirus subunit vaccine
    Kelly Lyn Warfield; Fiscal Year: 2012
    ..In this proposal, using a novel approach, we seek to develop a vaccine that is easy to produce and would protect against several strains of filoviruses. ..
  35. Bacteria-Mucin Interactions at the Ocular Surface
    Ilene K Gipson; Fiscal Year: 2012
    ..in the absence of known or suspected ocular surface trauma), interact with and affect membrane-associated mucins to gain access to epithelial cells...
  36. Molecular Pathways Regulating Airway Goblet Cell Mucin Secretion
    Burton F Dickey; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The secreted gel-forming mucins of the airway provide important protective functions through the clearance of inhaled pathogens and particulates;however, mucin hypersecretion causes airflow ..
  37. Novel Molecules in Airway Epithelial Endoplasmic Reticulum
    Bradley W Schroeder; Fiscal Year: 2012
    ..David Erle. Excessive amounts of mucins, enormous glycoproteins that appear to be critical for the viscoelastic properties of mucus, contribute to ..
  38. Tim3 and Mycobacterium tuberculosis
    Samuel M Behar; Fiscal Year: 2013
    ..Does IL-1 and its downstream mediators activate human MX to kill Mtb? 7 Aim 3. How does the Tim3/Gal9 interaction modulate immunity to tuberculosis in vivo? ..
  39. Virulence in Enteroaggregative Escherichia coli
    James P Nataro; Fiscal Year: 2013
    ..In this project, we will further characterize the mechanisms of EAEC- induced diarrhea with an eye toward development of effective preventive measures. ..
  40. Effects of saliva on herpes simplex virus infection of oralcells
    CLAUDE F KRUMMENACHER; Fiscal Year: 2013
    ..data showing that jacalin depletes saliva of its activity suggest a role for carbohydrates on glycoproteins, mucins and/or IgA. Thus we will examine the role of carbohydrates and use this lectin to improve purification...
  41. Oxidative stress in asthma initiation
    Sanjiv Sur; Fiscal Year: 2010
    ..Future studies of POPAC may identify novel therapeutic approaches in asthma. ..
  42. Dual Oxidase in Airway Epithelial Injury and Inflammation
    Albert van Der Vliet; Fiscal Year: 2013
    ..Successful accomplishment of these studies would identify DUOX1 as a novel and attractive therapeutic target in the management of asthma and/or its exacerbations. ..
  43. Modulation of MUC1 Mucin Trafficking
    Rebecca P Hughey; Fiscal Year: 2010
    ..Our success in these studies will provide novel and fundamental insights into both MUC1-dependent signaling and mislocalization in tumors, which will contribute to a new level of understanding of MUC1 biology in human cancer. ..
  44. Interactions of Ebola virus glycoproteins with host cells
    Paul Bates; Fiscal Year: 2013
    ....
  45. Regulation of TH2 memory/effector cells during allergic inflammation
    Yui Hsi Wang; Fiscal Year: 2013
    ....
  46. Carbohydrate Antigenic Biomarkers for Epithelial Cancers
    Minoru Fukuda; Fiscal Year: 2013
    ..using glycan arrays generated from the prostate cancer cell line, PC-3, and from AE3 antigen-positive epithelial mucins. The second is cell transfection of specific glycosyltransferases and other glyco-modifying enzymes such as ..
  47. Serum biomarkers for early detection of H. pylori-associated gastric cancer
    Jay V Solnick; Fiscal Year: 2013
    ..abstract_text> ..
  48. Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
    Darlene A Dartt; Fiscal Year: 2013
    ..Agonists, antagonists, chemical inhibitors, and siRNA will be used to characterize responses. ..
  49. Development of human thioredoxin as a mucolytic therapy for Cystic Fibrosis
    Peter B Heifetz; Fiscal Year: 2012
    ..This mucus barrier is composed primarily of cross-linked chains of proteins (mucins) complexed with nucleic acids released as a consequence of persistent lung inflammation and infection...
  50. Molecular Markers for the Diagnosis of Pancreatic Cancer
    Surinder K Batra; Fiscal Year: 2012
    ..The proposed investigations are expected to improve the current diagnosis of pancreatic cancer and can easily be translated to clinics after successful multi-center evaluation. ..
  51. Regulation of NF-kappaB and IKK by proinflammatory stimuli
    Michael Karin; Fiscal Year: 2013
    ..The proposed studies will be based on a combination of biochemistry, cell biology, and mouse physiology, an integrated approach that was proven very effective in the past. ..
  52. FLOW EFFECTS ON ENDOTHELIAL/TROPHOBLAST INTERACTION
    Gordon C Douglas; Fiscal Year: 2013
    ..Aim 4 determines whether MUC1 modulates ER function by regulating the intracellular localization of ERs. The final aim uses an optical trap to determine whether cell surface MUC1 responds to directly applied force. ..
  53. The Role of MAP 3 kinase 1 in Ocular Surface Morphogenesis
    Ying Xia; Fiscal Year: 2012
    ..Information derived from this work will pave the way for further investigation of genetic and environmental interactions in ocular surface development and identification of the etiology of congenital corneal anomalies. ..
  54. Preventing fucose-dependent binding of aspergillus and pseudomonas to lung mucin
    John V Fahy; Fiscal Year: 2012
    ..In this way we will prevent microbial adhesion to mucins and mucin-covered cell surfaces, and we will inhibit the mucin cross-linking caused by these multimeric lectins...
  55. Pulmonary Formulation of tPA for Plastic Bronchitis
    Kathleen A Stringer; Fiscal Year: 2010
    ..The information we acquire from this project is relevant to public health because it will improve the health and care of severely ill children. ..
  56. Modulation of conjunctival goblet cell differentiation by immunoregulatory cells
    Cintia Sade de Paiva; Fiscal Year: 2010
    ..These studies will provide new information about natural mechanisms to control inflammation on the ocular surface and may provide new insight into treating ocular surface inflammatory diseases. ..
  57. MUCINS OF THE OCULAR SURFACE
    Ilene K Gipson; Fiscal Year: 2013
    ....
  58. The Pathobiology of Airway Disease in Alpha-1 Antitrypsin Deficiency
    Michael A Campos; Fiscal Year: 2010
    ..abstract_text> ..
  59. Functional Study of Mucin MUC1
    Sandra J Gendler; Fiscal Year: 2013
    ..Results from these studies should give us clearer insight into the role of MUC1 in the epithelium and hematopoietic system in both inflammation and cancer signaling pathways. ..
  60. Up-regulation of Mucin Gene Transcription-Otitis Media
    Jian dong Li; Fiscal Year: 2013
    ..Overall, these studies will unveil novel molecular mechanisms underlying mucin up-regulation and identify novel therapeutic targets, leading to new therapeutic strategy for inhibiting mucus overproduction in OM (significance). ..
  61. The Impact of Tobacco Exposure on the Lungs Innate Defense System
    Robert Tarran; Fiscal Year: 2013
    ..Such knowledge may help dispel the myth that new tobacco products represent a safer alternative to cigarettes. ..
  62. Molecular mechanisms of squamous metaplasia in dry eye
    Nancy A McNamara; Fiscal Year: 2013
    ..ocular surface to a keratinized, "skin-like" epithelium;and (2) disruption of ocular surface and goblet cell mucins. This process, known as squamous metaplasia (SQM), is a devastating, end-stage consequence of dry eye disease that ..
  63. Dependency of O-3 Induced Lung Mucus Hypersecretion on NQ01
    W Michael Foster; Fiscal Year: 2010
    ..NQO1, generates reactive oxygen metabolites and leads to an increase in MUC5AC mRNA expression and production of mucins by airway epithelial cells...
  64. REGULATION OF GLANDULAR MUCOUS CELL SECRETION
    DAVID CULP; Fiscal Year: 2001
    ..b>Mucins are secreted in large part by mucous glands (e.g...
  65. DISTRIBUTION AND TRANSPORT OF LIPIDS IN MUCIN GELS
    Timothy Wiedmann; Fiscal Year: 2000
    ..Moreover, elucidation of the limiting physical chemistry of lipids and mucin will form the basis for the structure/function of mucous membranes throughout the body. ..
  66. METASTASIS SPECIFIC CARBOHYDRATE ANTIGENS
    Reuben Lotan; Fiscal Year: 1991
    The amount of sialyl-dimeric LeX antigen (NeuAc2-3Ga11-4(Fuc1- 3)GlcNAc1-3Gal1-4(Fuc1-3)GlcNAc1-) expressed on mucins in human colorectal carcinoma tissues increases upon tumors' progression to the metastatic phenotype...
  67. MOLECULAR ANALYSIS OF MUCIN TYPE O GLYCOSYLATION
    Michael Hollingsworth; Fiscal Year: 2000
    ..The substrates to be used include tandem repeats from different mucins and from mucin-like proteins such as the p-selection ligand PSGL-1...
  68. REGULATION OF TRACHEA MUCIN SYNTHESIS AND GLYCOSYLATION
    Joseph Mendicino; Fiscal Year: 1990
    ..These preliminary studies provide considerable encouragement for success of the studies described in this application...
  69. Trophoblast Selectins and Their Uterine Mucin Ligands
    Susan Fisher; Fiscal Year: 2006
    ..MUC7 mediates neutrophil adhesion in the range of shear stresses encountered at oral surfaces, a noel function for mucins. In this context we noted work from other groups that shows uterine expression of MUC1, which also carries sialyl ..
  70. T. gondii as a model for investigation of Cryptosporidium glycoprotein antigens
    ROBERTA M O apos CONNOR; Fiscal Year: 2010
    ..To address the need for an expression system that mimics post-translational modifications of the native Cp mucins, we expressed Cp glycoproteins in the related apicomplexan Toxoplasma gondii (Tg)...
  71. DEVELOPMENT OF CELL LINES FROM SUBLINGUAL GLANDS
    DAVID CULP; Fiscal Year: 1992
    b>Mucins are the primary secretory component of salivary mucous glands, such as the major sublingual gland and many of the minor salivary glands...