glycopeptides

Summary

Summary: Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.

Top Publications

  1. ncbi Assay for the measurement of copeptin, a stable peptide derived from the precursor of vasopressin
    Nils G Morgenthaler
    Research Department, B R A H M S AG, Biotechnology Centre Hennigsdorf Berlin, Germany
    Clin Chem 52:112-9. 2006
  2. ncbi Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides
    Johannes Stadlmann
    Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Proteomics 8:2858-71. 2008
  3. ncbi Cotton HILIC SPE microtips for microscale purification and enrichment of glycans and glycopeptides
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Anal Chem 83:2492-9. 2011
  4. ncbi Enrichment of glycopeptides for glycan structure and attachment site identification
    Jonas Nilsson
    Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden
    Nat Methods 6:809-11. 2009
  5. ncbi Copeptin: a novel, independent prognostic marker in patients with ischemic stroke
    Mira Katan
    Department of Endocrinology, University Hospital Basel, Switzerland
    Ann Neurol 66:799-808. 2009
  6. ncbi Incremental value of copeptin for rapid rule out of acute myocardial infarction
    Tobias Reichlin
    Department of Internal Medicine, University Hospital, Basel, Switzerland
    J Am Coll Cardiol 54:60-8. 2009
  7. ncbi Utilizing ion-pairing hydrophilic interaction chromatography solid phase extraction for efficient glycopeptide enrichment in glycoproteomics
    Simon Mysling
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230, Denmark
    Anal Chem 82:5598-609. 2010
  8. ncbi Association of copeptin and N-terminal proBNP concentrations with risk of cardiovascular death in older patients with symptoms of heart failure
    Urban Alehagen
    Division of Cardiovascular Medicine, Department of Medicine and Health Sciences, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    JAMA 305:2088-95. 2011
  9. pmc Cell wall thickening is a common feature of vancomycin resistance in Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 41:5-14. 2003
  10. ncbi C-terminal provasopressin (copeptin) is a strong prognostic marker in patients with heart failure after an acute myocardial infarction: results from the OPTIMAAL study
    Adriaan A Voors
    Department of Cardiology, University Medical Center Groningen, PO Box 30 001, 9700 RB Groningen, The Netherlands
    Eur Heart J 30:1187-94. 2009

Detail Information

Publications357 found, 100 shown here

  1. ncbi Assay for the measurement of copeptin, a stable peptide derived from the precursor of vasopressin
    Nils G Morgenthaler
    Research Department, B R A H M S AG, Biotechnology Centre Hennigsdorf Berlin, Germany
    Clin Chem 52:112-9. 2006
    ..We present a method for quantifying AVP release by use of copeptin, a glycopeptide comprising the C-terminal part of the AVP prohormone...
  2. ncbi Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides
    Johannes Stadlmann
    Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Proteomics 8:2858-71. 2008
    Two LC-ESI-MS methods for the analysis of antibody glycosylation are presented. In the first approach, tryptic glycopeptides are separated by RP chromatography and analyzed by ESI-MS...
  3. ncbi Cotton HILIC SPE microtips for microscale purification and enrichment of glycans and glycopeptides
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Anal Chem 83:2492-9. 2011
    ..The performance of the tips was evaluated for microscale purification of tryptic IgG Fc N-glycopeptides. Cotton wool HILIC SPE microtips allowed the removal of salts, most nonglycosylated peptides, and detergents ..
  4. ncbi Enrichment of glycopeptides for glycan structure and attachment site identification
    Jonas Nilsson
    Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden
    Nat Methods 6:809-11. 2009
    ..We identified 36 N-linked and 44 O-linked glycosylation sites on glycoproteins from human cerebrospinal fluid...
  5. ncbi Copeptin: a novel, independent prognostic marker in patients with ischemic stroke
    Mira Katan
    Department of Endocrinology, University Hospital Basel, Switzerland
    Ann Neurol 66:799-808. 2009
    ..Vasopressin is a stress hormone. Its production rate is mirrored in circulating levels of copeptin, a fragment of provasopressin. We evaluated the prognostic value of copeptin in acute stroke patients...
  6. ncbi Incremental value of copeptin for rapid rule out of acute myocardial infarction
    Tobias Reichlin
    Department of Internal Medicine, University Hospital, Basel, Switzerland
    J Am Coll Cardiol 54:60-8. 2009
    ..The purpose of this study was to examine the incremental value of copeptin for rapid rule out of acute myocardial infarction (AMI)...
  7. ncbi Utilizing ion-pairing hydrophilic interaction chromatography solid phase extraction for efficient glycopeptide enrichment in glycoproteomics
    Simon Mysling
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230, Denmark
    Anal Chem 82:5598-609. 2010
    ..The method involves TFA ion pairing (IP) to increase the hydrophilicity difference between glycopeptides and nonglycosylated peptides. Three mobile phases were investigated, i.e...
  8. ncbi Association of copeptin and N-terminal proBNP concentrations with risk of cardiovascular death in older patients with symptoms of heart failure
    Urban Alehagen
    Division of Cardiovascular Medicine, Department of Medicine and Health Sciences, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    JAMA 305:2088-95. 2011
    ..Measurement of plasma concentrations of the biomarker copeptin may help identify patients with heart failure at high and low risk of mortality, although the value of copeptin measurement in elderly patients is not fully known...
  9. pmc Cell wall thickening is a common feature of vancomycin resistance in Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 41:5-14. 2003
    ..Cell wall thicknesses and MICs of glycopeptides (vancomycin and teicoplanin) and beta-lactams (imipenem and oxacillin) were determined for a total of 48 ..
  10. ncbi C-terminal provasopressin (copeptin) is a strong prognostic marker in patients with heart failure after an acute myocardial infarction: results from the OPTIMAAL study
    Adriaan A Voors
    Department of Cardiology, University Medical Center Groningen, PO Box 30 001, 9700 RB Groningen, The Netherlands
    Eur Heart J 30:1187-94. 2009
    ....
  11. ncbi Copeptin improves early diagnosis of acute myocardial infarction
    Till Keller
    Department of Medicine II, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
    J Am Coll Cardiol 55:2096-106. 2010
    ..Early identification of myocardial infarction in chest pain patients is crucial to identify patients at risk and to maintain a fast treatment initiation...
  12. pmc Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry
    Tao Liu
    Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, USA
    J Proteome Res 4:2070-80. 2005
    ..captured from the depleted plasma using hydrazide resin and enzymatically digested, and the bound N-linked glycopeptides were released using peptide-N-glycosidase F (PNGase F)...
  13. pmc Combined determination of highly sensitive troponin T and copeptin for early exclusion of acute myocardial infarction: first experience in an emergency department of a general hospital
    Ulrich Lotze
    Department of Internal Medicine, German Red Cross Hospital Sondershausen, Sondershausen, Germany
    Vasc Health Risk Manag 7:509-15. 2011
    ....
  14. ncbi Changes in plasma copeptin, the c-terminal portion of arginine vasopressin during water deprivation and excess in healthy subjects
    Gabor Szinnai
    Division of Endocrinology, Diabetology, and Clinical Nutrition, University Hospital, CH 4031 Basel, Switzerland
    J Clin Endocrinol Metab 92:3973-8. 2007
    ..Copeptin is the C-terminal part of provasopressin and has been shown to be a useful tool to indicate AVP concentration in critically ill patients...
  15. pmc Comparison of the temporal release pattern of copeptin with conventional biomarkers in acute myocardial infarction
    Youlan L Gu
    Department of Cardiology, Thorax Center, University Medical Center Groningen, University of Groningen, Hanzeplein 1, PO Box 30001, 9700 RB, Groningen, The Netherlands
    Clin Res Cardiol 100:1069-76. 2011
    ....
  16. pmc Hypervirulence of a rough variant of the Mycobacterium abscessus type strain
    E Catherinot
    Laboratoire de Microbiologie, Hopital Raymond Poincare, 104 Bd Raymond Poincaré, 92380 Garches, France
    Infect Immun 75:1055-8. 2007
    ..We show that this variant has lost the ability to produce glycopeptidolipids, is hyperlethal for C57BL/6 mice infected intravenously, and induces a strong tumor necrosis factor-alpha response by murine monocyte-derived macrophages...
  17. ncbi Plasma copeptin, a unifying factor behind the metabolic syndrome
    Sofia Enhörning
    Department of Clinical Science, Lund University, Malmo University Hospital, SE 205 02 Malmo, Sweden
    J Clin Endocrinol Metab 96:E1065-72. 2011
    ..We previously showed that high copeptin, the stable C-terminal fragment of AVP prohormone, is independently associated with hyperinsulinemia and future development of diabetes mellitus...
  18. pmc Agricultural antibiotics and human health
    David L Smith
    Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Med 2:e232. 2005
  19. ncbi Elucidation of glycoprotein structures by unspecific proteolysis and direct nanoESI mass spectrometric analysis of ZIC-HILIC-enriched glycopeptides
    Kristina Neue
    Institute of Medical Physics and Biophysics, University of Münster, Münster, Germany
    J Proteome Res 10:2248-60. 2011
    Protein glycosylation was explored by direct nanoESI MS and MS/MS analysis of ZIC-HILIC-enriched proteolytic glycopeptides without further separation or purification...
  20. ncbi Copeptin, a surrogate marker of vasopressin, is associated with accelerated renal function decline in renal transplant recipients
    Esther Meijer
    Division of Nephrology, Department of Internal Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Transplantation 88:561-7. 2009
    ..Whether endogenous VP is a renal risk factor in humans has not been investigated yet. We aimed to investigate whether, in renal transplant recipients, VP concentration is associated with change in renal function during follow-up...
  21. ncbi Correlation of plasma copeptin and vasopressin concentrations in hypo-, iso-, and hyperosmolar States
    Sandrina Balanescu
    Clinic of Internal Medicine, Hospital Center, Vogelsang 84, CH 2501 Biel Bienne, Switzerland
    J Clin Endocrinol Metab 96:1046-52. 2011
    ..However, it is unknown to date how circulating copeptin and vasopressin levels correlate at different plasma osmolalites...
  22. ncbi Combined testing of high-sensitivity troponin T and copeptin on presentation at prespecified cutoffs improves rapid rule-out of non-ST-segment elevation myocardial infarction
    Evangelos Giannitsis
    Medizinische Klinik, Abteilung für Innere Medizin III, Universitatsklinikum Heidelberg, Heidelberg, Germany
    Clin Chem 57:1452-5. 2011
    ....
  23. pmc Copeptin, a surrogate marker of vasopressin, is associated with disease severity in autosomal dominant polycystic kidney disease
    Esther Meijer
    Division of Nephrology, Department of Internal Medicine, University Medical Center Groningen, PO Box 30 001, 9700 RB Groningen, The Netherlands
    Clin J Am Soc Nephrol 6:361-8. 2011
    ..However, it is unknown whether endogenous vasopressin concentration is associated with disease severity in patients with ADPKD...
  24. ncbi Highly specific enrichment of glycopeptides using boronic acid-functionalized mesoporous silica
    Yawei Xu
    Department of Chemistry and Institutes of Biomedical Sciences and Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, P R China
    Anal Chem 81:503-8. 2009
    ..holds the attractive features of high surface area and large accessible porosity, was developed to enrich glycopeptides. This is the first time that mesoporous material has been introduced into glycoproteome...
  25. ncbi Gender and renal function influence plasma levels of copeptin in healthy individuals
    Sanjay S Bhandari
    Department of Cardiovascular Sciences, University of Leicester, Leicester LE2 7LX, U K
    Clin Sci (Lond) 116:257-63. 2009
    ..The present study suggests that gender and renal function specific partition values should be used to interpret copeptin values in future studies of this biomarker in HF or ischaemic heart disease...
  26. ncbi Vasopressin beyond water: implications for renal diseases
    Davide Bolignano
    CNR IBIM, Clinical Epidemiology and Physiopathology of Renal Diseases and Hypertension of Reggio Calabria, Reggio Calabria, Italy
    Curr Opin Nephrol Hypertens 19:499-504. 2010
    ..This review aims to analyze the relationships between arginine vasopressin (AVP) and chronic kidney disease (CKD) and to define the potential of vasopressin receptor antagonists beyond the treatment of water metabolism disorders...
  27. pmc Mechanisms of resistance and clinical relevance of resistance to β-lactams, glycopeptides, and fluoroquinolones
    Louis B Rice
    Department of Medicine, Warren Alpert Medical School of Brown University, Providence, RI, USA
    Mayo Clin Proc 87:198-208. 2012
    ..Antimicrobial classes for which resistance has become a major problem include the β-lactams, the glycopeptides, and the fluoroquinolones...
  28. ncbi Combined copeptin and troponin to rule out myocardial infarction in patients with chest pain and a history of coronary artery disease
    Patrick Ray
    Department of Emergency Medicine, Pitie Salpetriere Hospital, Assistance Publique Hopitaux de Paris AP HP, INSERM UMRS 956, Universite Pierre et Marie Curie Paris 6, 75013, Paris, France
    Am J Emerg Med 30:440-8. 2012
    ....
  29. pmc Biosynthesis and structure of aeruginoside 126A and 126B, cyanobacterial peptide glycosides bearing a 2-carboxy-6-hydroxyoctahydroindole moiety
    Keishi Ishida
    Institute of Biology, Humboldt University Berlin, 10115 Berlin, Germany
    Chem Biol 14:565-76. 2007
    ..Insertional mutagenesis of a NRPS component led to the discovery and structural elucidation of two glycopeptides that were designated aeruginoside 126A and aeruginoside 126B...
  30. ncbi Increased 90-day mortality in patients with acute heart failure with elevated copeptin: secondary results from the Biomarkers in Acute Heart Failure (BACH) study
    Alan Maisel
    VA San Diego Healthcare System, San Diego, CA, USA
    Circ Heart Fail 4:613-20. 2011
    ..However, AVP is difficult to measure due to its in vitro instability and rapid clearance. Copeptin, the C-terminal segment of preprovasopressin, is a stable and reliable surrogate biomarker for serum arginine vasopressin concentrations...
  31. ncbi GlycoSpectrumScan: fishing glycopeptides from MS spectra of protease digests of human colostrum sIgA
    Nandan Deshpande
    Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, New South Wales, 2109, Australia
    J Proteome Res 9:1063-75. 2010
    With the emergence of glycoproteomics, there is a need to develop bioinformatic tools to identify glycopeptides in protease digests of glycoproteins...
  32. pmc Linezolid vs glycopeptide antibiotics for the treatment of suspected methicillin-resistant Staphylococcus aureus nosocomial pneumonia: a meta-analysis of randomized controlled trials
    Allan J Walkey
    Boston University School of Medicine, The Pulmonary Center, 715 Albany St, R 304, Boston, MA 02118, USA
    Chest 139:1148-55. 2011
    ..Societal guidelines suggest linezolid may be the preferred treatment of MRSA nosocomial pneumonia. We investigated the efficacy of linezolid compared with glycopeptide antibiotics (vancomycin or teicoplanin) for nosocomial pneumonia...
  33. ncbi Structural characterization of N-linked oligosaccharides on monoclonal antibody cetuximab by the combination of orthogonal matrix-assisted laser desorption/ionization hybrid quadrupole-quadrupole time-of-flight tandem mass spectrometry and sequential enzy
    Jun Qian
    Department of Bioanalytical Science, ImClone Systems Incorporated, Branchburg, NJ 08876, USA
    Anal Biochem 364:8-18. 2007
    ..This study represents a unique approach in that MS/MS data were used to identify and confirm the oligosaccharide structures of a protein...
  34. ncbi Spontaneous reversion of Mycobacterium abscessus from a smooth to a rough morphotype is associated with reduced expression of glycopeptidolipid and reacquisition of an invasive phenotype
    Susan T Howard
    Center for Pulmonary and Infectious Disease Control, University of Texas Health Center at Tyler, Tyler, TX 75708, USA
    Microbiology 152:1581-90. 2006
    ..These results indicate that the ability to switch between smooth and rough morphologies may allow M. abscessus to transition between a colonizing phenotype and a more virulent, invasive form...
  35. ncbi Copeptin, C-reactive protein, and procalcitonin as prognostic biomarkers in acute exacerbation of COPD
    Daiana Stolz
    Clinic of Pneumology and Pulmonary Cell Research, University Hospital Basel, Petersgraben 4, CH 4031 Basel, Switzerland
    Chest 131:1058-67. 2007
    ....
  36. ncbi High throughput quantitative analysis of serum proteins using glycopeptide capture and liquid chromatography mass spectrometry
    Hui Zhang
    Institute for Systems Biology, Seattle, WA 98103, USA
    Mol Cell Proteomics 4:144-55. 2005
    ..By focusing selectively on a few formerly N-linked glycopeptides per serum protein, the complexity of the analyte sample is significantly reduced and the sensitivity and ..
  37. pmc Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis
    Catherine Vilcheze
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Microbiol 69:1316-29. 2008
    ..Mutations in mshA demonstrate the non-essentiality of mycothiol for growth in vitro and in vivo, and provide a novel mechanism of ethionamide resistance in M. tuberculosis...
  38. pmc O-glycosylation modulates proprotein convertase activation of angiopoietin-like protein 3: possible role of polypeptide GalNAc-transferase-2 in regulation of concentrations of plasma lipids
    Katrine T B G Schjoldager
    Center for Glycomics, Department of Cellular and Molecular Medicine and School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK 2200 Copenhagen N, Denmark
    J Biol Chem 285:36293-303. 2010
    ..The study demonstrates that ANGPTL3 activation is modulated by O-glycosylation and that this step is probably controlled by GalNAc-T2...
  39. ncbi Copeptin: clinical use of a new biomarker
    Nils G Morgenthaler
    Research Department, B R A H M S AG, Biotechnology Centre, Henningsdorf Berlin, Germany
    Trends Endocrinol Metab 19:43-9. 2008
    ..Here we review recent findings regarding the relationship between AVP and copeptin, and affirm the value of AVP as a surrogate marker for AVP...
  40. pmc The mycobacterial glycopeptidolipids: structure, function, and their role in pathogenesis
    Jeffrey S Schorey
    Department of Biological Sciences, Eck Institute for Global Health and Infectious Diseases, University of Notre Dame, Notre Dame, IN 46556, USA
    Glycobiology 18:832-41. 2008
    ..These topics, along with new information on the enzymes involved in GPL biosynthesis, are the subject of this review...
  41. ncbi Copeptin, a stable peptide derived from the vasopressin precursor, is elevated in serum of sepsis patients
    Joachim Struck
    Department of Research, BRAHMS Aktiengesellschaft, Neuendorfstr 25, D 16761 Hennigsdorf, Germany
    Peptides 26:2500-4. 2005
    ....
  42. ncbi Cancer biomarkers defined by autoantibody signatures to aberrant O-glycopeptide epitopes
    Hans H Wandall
    Center for Glycomics, Departments of Cellular and Molecular Medicine and Oral Diagnostics, University of Copenhagen, Blegdamsvej 3, Copenhagen 2200 N, Denmark
    Cancer Res 70:1306-13. 2010
    ..The methods we have developed for chemoenzymatic synthesis of O-glycopeptides on microarrays may allow for broader mining of the entire cancer O-glycopeptidome.
  43. ncbi Identification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice
    Yasuhiro Yamaguchi
    Division of Integrative Cell Biology, Department of Embryogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University, Japan
    J Immunol 169:2516-23. 2002
    ..Our findings indicated that multiple beta-defensin isoforms specifically and cooperatively contribute to the innate immunity of the urogenital system...
  44. ncbi Hydrophilic affinity isolation and MALDI multiple-stage tandem mass spectrometry of glycopeptides for glycoproteomics
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    Anal Chem 76:6560-5. 2004
    ..Considering that structural issues are elucidated by studying glycopeptides and that the tandem MS of a tryptic peptide composed of several amino acid residues is enough for protein ..
  45. pmc Plasma copeptin and the risk of diabetes mellitus
    Sofia Enhörning
    Department of Clinical Sciences, Lund University, Clinical Research Center, Entrance 72, Bldg 91, Floor 12, Malmo University Hospital, SE 20502 Malmo, Sweden
    Circulation 121:2102-8. 2010
    ..Animal studies suggest that the arginine vasopressin system may play a role in glucose metabolism, but data from humans are limited...
  46. ncbi Copeptin: a biomarker of cardiovascular and renal function
    Nils G Morgenthaler
    From the Institut für Experimentelle Endokrinologie und Endokrinologisches Forschungszentrum, EnForCé, Charite, Berlin, Germany
    Congest Heart Fail 16:S37-44. 2010
    ....
  47. ncbi GlycoMod--a software tool for determining glycosylation compositions from mass spectrometric data
    C A Cooper
    Proteome Systems Ltd, Locked Bag 2073, North Ryde, Sydney, NSW 1670, Australia
    Proteomics 1:340-9. 2001
    ..Furthermore, known oligosaccharide structural constraints on monosaccharide composition are also incorporated into the program to limit the output...
  48. ncbi Copeptin, a fragment of the vasopressin precursor, as a novel predictor of outcome in heart failure
    B Stoiser
    Medical University of Vienna, Austria
    Eur J Clin Invest 36:771-8. 2006
    ..Vasopressin is also known to be related to the severity of heart disease. Copeptin--an inactive fragment of the vasopressin precursor--has not been previously investigated in the context of heart failure...
  49. ncbi Copeptin, a surrogate marker of vasopressin, is associated with microalbuminuria in a large population cohort
    Esther Meijer
    Department of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Kidney Int 77:29-36. 2010
    ..If future studies show that this association is causal, then drinking more water or pharmacological intervention to decrease plasma vasopressin may have beneficial effects on the kidney, especially in the elderly...
  50. pmc Plasma carboxy-terminal provasopressin (copeptin): a novel marker of insulin resistance and metabolic syndrome
    Umer Saleem
    Division of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Clin Endocrinol Metab 94:2558-64. 2009
    ..Stress-mediated hypothalamic-pituitary-adrenal axis activation, regulated by arginine vasopressin (AVP), may have a role in the pathophysiology of metabolic syndrome (MetSyn)...
  51. ncbi Mucin-type O-glycosylation--putting the pieces together
    Pia H Jensen
    Department of Chemistry and Biomolecular Sciences, Faculty of Science, Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia
    FEBS J 277:81-94. 2010
    ....
  52. ncbi Plasma copeptin levels before and during exogenous arginine vasopressin infusion in patients with advanced vasodilatory shock
    C Torgersen
    Department of Intensive Care Medicine, Bern University Hospital, Bern, Switzerland
    Minerva Anestesiol 76:905-12. 2010
    ....
  53. ncbi The role of glycosylation in protein antigenic properties
    E Lisowska
    Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław
    Cell Mol Life Sci 59:445-55. 2002
    ..Most data have been obtained from model studies on glycoprotens with well-defined structure or synthetic glycopeptides and their respective monoclonal antibodies...
  54. ncbi Prognostic value of copeptin: one-year outcome in patients with traumatic brain injury
    Guo Feng Yu
    Department of Neurosurgery, Quzhou People s Hospital, 2 Zhongloudi Road, Kecheng District, Quzhou 324000, China
    Peptides 33:164-9. 2012
    ..Thus, copeptin level is a useful, complementary tool to predict functional outcome and mortality 1 year after traumatic brain injury...
  55. pmc Copeptin, IGFBP-1, and cardiovascular prognosis in patients with type 2 diabetes and acute myocardial infarction: a report from the DIGAMI 2 trial
    Linda G Mellbin
    Department of Medicine, Karolinska Institutet, Stockholm, Sweden
    Diabetes Care 33:1604-6. 2010
    ..To determine whether C-terminal provasopressin (copeptin) explains the prognostic importance of insulin growth factor binding protein-1 (IGFBP-1) in patients with myocardial infarction and type 2 diabetes...
  56. ncbi Glycopeptide antibiotics: from conventional molecules to new derivatives
    Francoise Van Bambeke
    Unité de Pharmacologie Cellulaire et Moléculaire, Universite Catholique de Louvain, 73 70 avenue Mounier 73, Brussels 1200, Belgium
    Drugs 64:913-36. 2004
    ..In parallel, considerable efforts have been made to produce semisynthetic glycopeptides with improved pharmacokinetic and pharmacodynamic properties, and with activity towards resistant strains...
  57. pmc Functional characterization of the sigmaB-dependent yabJ-spoVG operon in Staphylococcus aureus: role in methicillin and glycopeptide resistance
    Bettina Schulthess
    Institute of Medical Microbiology, University of Zurich, Gloriastr 32, 8006 Zurich, Switzerland
    Antimicrob Agents Chemother 53:1832-9. 2009
    ..We therefore postulate that SpoVG is the major factor of the yabJ-spoVG operon required in S. aureus for capsule formation and antibiotic resistance...
  58. ncbi The use of copeptin, the stable peptide of the vasopressin precursor, in the differential diagnosis of sodium imbalance in patients with acute diseases
    Nicole Nigro
    Department of Endocrinology, University Hospital Basel, Basel, CH, Switzerland
    Swiss Med Wkly 141:w13270. 2011
    ..This study assessed copeptin concentrations in sick patients with serum sodium imbalance of different aetiology on admission to the emergency department...
  59. ncbi Evaluation of the Etest GRD for the detection of Staphylococcus aureus with reduced susceptibility to glycopeptides
    Steven N Leonard
    Anti Infective Research Laboratory, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA
    J Antimicrob Chemother 63:489-92. 2009
    ..The objective of this study was to compare a new method for detection of hVISA, the Etest GRD, to PAP-AUC and to macro Etest...
  60. pmc D-Ala-D-Ala ligases from glycopeptide antibiotic-producing organisms are highly homologous to the enterococcal vancomycin-resistance ligases VanA and VanB
    C G Marshall
    Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5
    Proc Natl Acad Sci U S A 94:6480-3. 1997
    ..lividans transformed with the ddl gene and confirms the predicted enzymatic activity. These results imply a close evolutionary relationship between resistance mechanisms in the clinics and in drug-producing bacteria...
  61. ncbi On-plate-selective enrichment of glycopeptides using boronic acid-modified gold nanoparticles for direct MALDI-QIT-TOF MS analysis
    Jia Tang
    Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, P R China
    Proteomics 9:5046-55. 2009
    ..These spots were used to selectively capture glycopeptides from peptide mixtures and the captured target peptides could be analyzed by MALDI-MS simply by deposition of 2,..
  62. ncbi Structure of human neutral endopeptidase (Neprilysin) complexed with phosphoramidon
    C Oefner
    Pharma Preclinical Research, F Hoffmann La Roche Ltd, Basel, CH 4070, Switzerland
    J Mol Biol 296:341-9. 2000
    ..The inhibitor is bound to one side of this cavity and its binding mode provides a detailed understanding of the ligand-binding and specificity determinants...
  63. ncbi Mass spectrometric glycan rearrangements
    Manfred Wuhrer
    Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden, The Netherlands
    Mass Spectrom Rev 30:664-80. 2011
    ..Similar phenomena have been reported for glycopeptides, labeled glycan structures and other biomolecules containing a carbohydrate part...
  64. ncbi Selective enrichment of sialic acid-containing glycopeptides using titanium dioxide chromatography with analysis by HILIC and mass spectrometry
    Giuseppe Palmisano
    Department of Biochemistry and Molecular Biology, The University of Southern Denmark, Odense, Denmark
    Nat Protoc 5:1974-82. 2010
    ..In this paper, we describe a protocol for the selective enrichment of SA-containing glycopeptides using a combination of titanium dioxide (TiO(2)) and hydrophilic interaction liquid chromatography (HILIC)...
  65. pmc Detection of copeptin in peripheral blood of patients with aneurysmal subarachnoid hemorrhage
    Xiang dong Zhu
    Department of Neurosurgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310000, PR China
    Crit Care 15:R288. 2011
    ..Copeptin has been proposed as a prognostic marker in acute illness. This study investigated the ability of copeptin to predict the disease outcome and cerebrovasospasm in the patients with aneurysmal subarachnoid hemorrhage...
  66. ncbi Resistance or decreased susceptibility to glycopeptides, daptomycin, and linezolid in methicillin-resistant Staphylococcus aureus
    Esteban Nannini
    Division of Infectious Diseases, School of Medicine, Universidad Nacional de Rosario, Rosario, Argentina
    Curr Opin Pharmacol 10:516-21. 2010
    Methicillin-resistant Staphylococcus aureus (MRSA) with decreased susceptibility to glycopeptides can be categorized as first, heteroresistant to vancomycin (hVISA); second, with intermediate susceptibility to vancomycin (VISA); and ..
  67. pmc Mutational analysis of cell wall biosynthesis in Mycobacterium avium
    Jean Pierre Laurent
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Bacteriol 185:5003-6. 2003
    ..Nineteen such mutants were isolated and mapped, including 10 with insertions in a functional island of cell wall biosynthetic genes that spans approximately 40 kb of the M. avium genome...
  68. pmc Antibacterial properties of the sperm-binding proteins and peptides of human epididymis 2 (HE2) family; salt sensitivity, structural dependence and their interaction with outer and cytoplasmic membranes of Escherichia coli
    Suresh Yenugu
    Laboratories for Reproductive Biology, Department of Pediatrics, University of North Carolina, Chapel Hill 27599 7500, USA
    Biochem J 372:473-83. 2003
    ..coli membranes. A much longer exposure time was required for human beta-defensin-1-mediated permeabilization of membranes, suggesting a possible difference in mode of action compared with the HE2 antibacterial peptides...
  69. ncbi Mycobacterium abscessus Glycopeptidolipids mask underlying cell wall phosphatidyl-myo-inositol mannosides blocking induction of human macrophage TNF-alpha by preventing interaction with TLR2
    Elizabeth R Rhoades
    Cornell Nanoscale Science and Technology Facility, Cornell University, Ithaca, NY 14853, USA
    J Immunol 183:1997-2007. 2009
    ..abscessus cell wall masks underlying cell wall lipids involved in stimulating the innate immune response, thereby facilitating colonization. Conversely spontaneous "unmasking" of cell wall lipids may promote airway inflammation...
  70. ncbi Quantitative determination of haptoglobin glycoform variants in psoriasis
    Bernardetta Maresca
    Dipartimento di Patologia Sistematica Sezione di Dermatologia, Universita di Napoli Federico II, Italy
    Biol Chem 391:1429-39. 2010
    ..and qualitative differences in glycan mass, between patients and controls, were found by mass spectrometry of glycopeptides from tryptic digests of protein isolated from both patients and controls...
  71. pmc Methylation of GPLs in Mycobacterium smegmatis and Mycobacterium avium
    Dharshini Jeevarajah
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    J Bacteriol 186:6792-9. 2004
    ..smegmatis methyltransferase mutants with M. avium genes revealed that MtfC and MtfB of the latter species have 4-O-methyltransferase activity and that MtfD is a 3-O-methyltransferase which can modify rhamnose of GPLs in M. smegmatis...
  72. ncbi Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins
    Judith Stepper
    Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
    FEBS Lett 585:645-50. 2011
    O-Glycosylation is a ubiquitous eukaryotic post-translational modification, whereas early reports of S-linked glycopeptides have never been verified...
  73. ncbi Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography
    Yasuhiro Takegawa
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001 0021, Japan
    J Chromatogr A 1113:177-81. 2006
    Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns...
  74. pmc Analysis of glycoproteins in human serum by means of glycospecific magnetic bead separation and LC-MALDI-TOF/TOF analysis with automated glycopeptide detection
    Katrin Sparbier
    Bruker Daltonik GmbH, Permoserstrasse 15, 04318 Leipzig, Germany
    J Biomol Tech 18:252-8. 2007
    ..selective capturing of glycopeptides and -proteins was attained by means of magnetic particles specifically functionalized with lectins or boronic ..
  75. ncbi Protein glycosylation analysis by HILIC-LC-MS of Proteinase K-generated N- and O-glycopeptides
    Gerhild Zauner
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    J Sep Sci 33:903-10. 2010
    ..The resulting glycopeptides were then resolved by nanoscale hydrophilic interaction liquid chromatography-electrospray multistage MS...
  76. ncbi Characterization of glycopeptides by combining collision-induced dissociation and electron-transfer dissociation mass spectrometry data
    William R Alley
    National Center for Glycomics and Glycoproteomics, Indiana University, Bloomington, IN 47405, USA
    Rapid Commun Mass Spectrom 23:161-70. 2009
    ..This is being achieved with a mass spectrometer capable of alternating between CID and ETD on-the-fly during an LC/MS/MS analysis. This is demonstrated here with several tryptic glycopeptides.
  77. pmc Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobact
    Nichollas E Scott
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Mol Cell Proteomics 10:M000031-MCP201. 2011
    ..We developed strategies for enriching C. jejuni HB93-13 glycopeptides using zwitterionic hydrophilic interaction chromatography and examined novel fragmentation, including ..
  78. pmc Sialic acid-focused quantitative mouse serum glycoproteomics by multiple reaction monitoring assay
    Masaki Kurogochi
    Graduate School of Life Science, Frontier Research Center for the Post Genomic Science and Technology, Hokkaido University, Kita ku, Sapporo, Japan
    Mol Cell Proteomics 9:2354-68. 2010
    ..analysis of de-glycosylated peptides identified 270 mouse serum peptides (95 glycoproteins) as sialylated glycopeptides, of which 67 glycopeptides were fully characterized by MS/MS analyses in a straightforward manner...
  79. ncbi Lipopentapeptide induces a strong host humoral response and distinguishes Mycobacterium avium subsp. paratuberculosis from M. avium subsp. avium
    Franck Biet
    UR1282, Infectiologie Animale, Santé Publique, INRA Centre de Tours, F 37380 Nouzilly, France
    Vaccine 26:257-68. 2008
    ..avium subsp. paratuberculosis (Map), the etiologic agent of Johne's disease in cattle, and several conflicting data have been produced...
  80. ncbi Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging
    Hiroyuki Kaji
    Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, Minamiosawa 1 1, Hachioji, Tokyo 192 0397, Japan
    Nat Protoc 1:3019-27. 2006
    ..The steps of this approach are: (1) lectin column-mediated affinity capture of glycopeptides generated by protease digestion of protein mixtures; (2) purification of the enriched glycopeptides by ..
  81. pmc Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis
    Yukari Amano
    Graduate School of Bio agricultural Sciences, Nagoya University, Chikusa, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 104:18333-8. 2007
    ..The present results suggest that this paralogous LRR-RK family integrates growth-promoting signals mediated by two structurally distinct sulfated peptides: PSY1 and PSK...
  82. ncbi Challenges of determining O-glycopeptide heterogeneity: a fungal glucanase model system
    Maja N Christiansen
    Biomolecular Frontiers Research Centre, Department of Chemistry and Bimolecular Sciences, Faculty of Science, Macquarie University, Sydney, NSW, 2109, Australia
    Anal Chem 82:3500-9. 2010
    ..The protease digests produced many glycopeptides as determined by CID-MS/MS, but ETD fragmentation of these resulted in only a few interpretable spectra, ..
  83. ncbi Quantitation of saccharide compositions of O-glycans by mass spectrometry of glycopeptides and its application to rheumatoid arthritis
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    J Proteome Res 9:1367-73. 2010
    ..and site-specific profiling of N-linked glycans of glycoproteins is conducted by mass spectrometry of glycopeptides. However, our knowledge of mucin-type O-glycans including site occupancy and profile variance, as well as ..
  84. pmc A glycosyltransferase involved in biosynthesis of triglycosylated glycopeptidolipids in Mycobacterium smegmatis: impact on surface properties
    Caroline Deshayes
    INSERM UMR570, Faculte de Medecine Necker Enfants Malades, 156 rue de Vaugirard, Paris Cedex 15, F 75730, France
    J Bacteriol 187:7283-91. 2005
    ..Phylogenetic analysis indicated that Gtf3 is a member of a yet-uncharacterized glycosyltransferase family conserved among the mycobacteria...
  85. ncbi Copeptin and peroxiredoxin-4 independently predict mortality in patients with nonspecific complaints presenting to the emergency department
    Christian H Nickel
    Emergency Department, University Hospital, Basel, Switzerland
    Acad Emerg Med 18:851-9. 2011
    ....
  86. ncbi Stress hormones predict cerebrovascular re-events after transient ischemic attacks
    M Katan
    Department of Endocrinology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
    Neurology 76:563-6. 2011
    ..This study assessed prognostic reliability of 2 distinct stress hormones, copeptin and cortisol, for the risk stratification of re-events in patients with TIA...
  87. ncbi The impact of the absence of glycopeptidolipids on the ultrastructure, cell surface and cell wall properties, and phagocytosis of Mycobacterium smegmatis
    Gilles Etienne
    Institut de Pharmacologie et de Biologie Structurale, Unité Mixte de Recherche du Centre de National de Recherche Scientifique et de l Université Paul Sabatier UMR 5089, 205 route de Narbonne, 31077 Toulouse Cedex 04, France
    Microbiology 148:3089-100. 2002
    ..smegmatis. Altogether, these data showed that, in addition to being distinctive markers of numerous mycobacterial species, GPLs play a role in the bacterial phenotype, surface properties and cell wall permeability...
  88. pmc A high-throughput O-glycopeptide discovery platform for seromic profiling
    Ola Blixt
    Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3b, Copenhagen, Denmark
    J Proteome Res 9:5250-61. 2010
    ..amine-reactive hydrogel-coated microarray glass surface, allowing high-throughput display of large numbers of glycopeptides. Utilizing a repertoire of recombinant glycosyltransferases enabled further diversification of the array ..
  89. ncbi Analysis of glycopeptides using lectin affinity chromatography with MALDI-TOF mass spectrometry
    Kazutosi Kubota
    Research Team for Functional Genomics and Research Team for Molecular Biomarkers, Tokyo Metropolitan Institute of Gerontology, Foundation for Research on Aging and Promotion of Human Welfare, 35 2 Sakae cho, Itabashi ku, Tokyo 173 0015, Japan
    Anal Chem 80:3693-8. 2008
    b>Glycopeptides prepared from 1 nmol of a mixture of glycoproteins, transferrin, and ribonuclease B by lysylendopeptidase digestion were isolated by lectin and cellulose column chromatographies, and then they were analyzed by matrix-..
  90. pmc Combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides
    Milan Madera
    Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA
    Anal Chem 77:4081-90. 2005
    ..The described analytical systems are amenable to the applications aiming at fractionation of complex glycopeptide mixtures and determination of the sites of glycosylation...
  91. ncbi Oxazole-modified glycopeptides that target arthritis-associated class II MHC A(q) and DR4 proteins
    Ida E Andersson
    Department of Chemistry, Umea University, SE 901 87 Umea, Sweden
    Org Biomol Chem 8:2931-40. 2010
    ..Here, we describe the design and synthesis of a small series of modified CII259-273 glycopeptides with oxazole heterocycles replacing three potentially labile peptide bonds...
  92. pmc Identification by genomic and genetic analysis of two new genes playing a key role in intermediate glycopeptide resistance in Staphylococcus aureus
    Adriana Renzoni
    Service of Infectious Diseases, Geneva University Hospital, Geneva, Switzerland
    Antimicrob Agents Chemother 53:903-11. 2009
    ..In conclusion, our study demonstrates the key role of two novel loci in endogenous, low-level glycopeptide resistance in S. aureus whose precise molecular functions warrant further investigation...
  93. ncbi Role of glycopeptides as part of initial empirical treatment of febrile neutropenic patients: a meta-analysis of randomised controlled trials
    Konstantinos Z Vardakas
    Alfa Institute of Biomedical Sciences, Athens, Greece
    Lancet Infect Dis 5:431-9. 2005
    We did a meta-analysis of randomised controlled trials studying glycopeptides as part of the initial empirical treatment of febrile neutropenic patients with a beta-lactam and with or without an aminoglycoside...
  94. ncbi Basic pharmacodynamics of antibacterials with clinical applications to the use of beta-lactams, glycopeptides, and linezolid
    William A Craig
    University of Wisconsin and William S Middleton Memorial VA Hospital, 2500 Overlook Terrace, Room D 2221, Madison, WI 53705, USA
    Infect Dis Clin North Am 17:479-501. 2003
    ..Penetration of these drugs into respiratory secretions, such as ELF, is enhanced for linezolid and reduced for vancomycin. This may give linezolid an advantage over vancomycin in certain respiratory infections...
  95. pmc Affinity enrichment and characterization of mucin core-1 type glycopeptides from bovine serum
    Zsuzsanna Darula
    Proteomics Research Group, Biological Research Center of the Hungarian Academy of Sciences, Szeged P O Box 521, Szeged H 6701, Hungary
    Mol Cell Proteomics 8:2515-26. 2009
    ..Structural elucidation by mass spectrometry is usually inconclusive as the CID spectra of most glycopeptides are dominated by carbohydrate-related fragments, preventing peptide identification...
  96. pmc Genetic changes associated with glycopeptide resistance in Staphylococcus aureus: predominance of amino acid substitutions in YvqF/VraSR
    Yoshihisa Kato
    Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd, Kohoku Ku, Yokohama, Japan
    J Antimicrob Chemother 65:37-45. 2010
    ..To further understand the mechanism of intermediate-level glycopeptide resistance, resulting from multiple endogenous mutations, in both laboratory-derived and clinically isolated Staphylococcus aureus...
  97. pmc Selective isolation of glycoproteins and glycopeptides for MALDI-TOF MS detection supported by magnetic particles
    Katrin Sparbier
    Bruker Daltonik GmbH, Leipzig, Germany
    J Biomol Tech 16:407-13. 2005
    ..According to the type of glycosylation (high man-nose, hybrid, or complex type) the different proteins were enriched by ConA or boronic acid-functionalized beads...
  98. ncbi Characterization of gel-separated glycoproteins using two-step proteolytic digestion combined with sequential microcolumns and mass spectrometry
    Martin R Larsen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense, Denmark
    Mol Cell Proteomics 4:107-19. 2005
    ..specific and nonspecific enzymatic treatment followed by selective purification and characterization of the glycopeptides using graphite powder microcolumns in combination with mass spectrometry...
  99. ncbi HE2beta and HE2gamma, new members of an epididymis-specific family of androgen-regulated proteins in the human
    K G Hamil
    Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill 27599 7500, USA
    Endocrinology 141:1245-53. 2000
    ..It is secreted into the lumen and binds to the sperm surface in the postacrosomal and neck regions. The beta2 form is expressed in principal cells primarily in efferent ducts...
  100. pmc An N-glucosylated peptide detecting disease-specific autoantibodies, biomarkers of multiple sclerosis
    Francesco Lolli
    Laboratory of Peptide and Protein Chemistry and Biology, Polo Scientifico, University of Florence, I 50019 Sesto Fiorentino, Italy
    Proc Natl Acad Sci U S A 102:10273-8. 2005
    ..Therefore, a CSF114(Glc)-based immunoassay on sera may have important prognostic value in monitoring MS disease progression guiding optimal therapeutic treatment...
  101. ncbi Copeptin in the differential diagnosis of hyponatremia
    Wiebke Fenske
    Endocrinology and Diabetes Unit, Department of Medicine I, University of Wurzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
    J Clin Endocrinol Metab 94:123-9. 2009
    ..This study was designed to evaluate the diagnostic potential of copeptin, the C-terminal part of provasopressin, as a new marker in the differential diagnosis of hyponatremia...

Research Grants64

  1. Photocleavable Bead Technology for Glycomics
    Mark Lim; Fiscal Year: 2013
    ..Large combinatorial bead-libraries of glycopeptides offer an alternative to microarrays, but normally utilize "panning" methods to measure interactions with the ..
  2. RAMP Mediated Gene Delivery
    Kevin G Rice; Fiscal Year: 2012
    ..RAMPs will be prepared on solid support by joining a melittin peptide, PEG-peptide and glycopeptides by disulfide bond formation...
  3. A fully synthetic carbohydrate-based cancer vaccine
    Geert Jan Boons; Fiscal Year: 2013
    ..candidates to establish strategies to overcome the poor immunogenicity of tumor-associated carbohydrates and glycopeptides. We have found that a three-component vaccine composed of a TLR2 agonist, a promiscuous peptide T-helper ..
  4. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2012
    ..autoantibodies in sera of patients with IgAN using a glycan microarray including various glycans and synthetic glycopeptides representing the IgA1 hinge region, and establish an ELISA method for potential diagnosis of IgAN from serum ..
  5. Novel Approaches to Access GPIs and GPI-Anchored Proteins for the Study of GPI An
    Zhongwu Guo; Fiscal Year: 2012
    ..Aim 2 is to develop a practical method for the chemical synthesis of natively linked GPI-anchored peptides/glycopeptides or proteins/glycoproteins...
  6. Alliance of Glycobiologists for Detection of Cancer
    Radoslav Goldman; Fiscal Year: 2013
    ..We propose to identify glycopeptides of additional proteins carrying these HCC specific N-glycan modifications by newly optimized mass ..
  7. Knowledge-based Vaccines Built on Paradigm Changes in Antigen Presentation
    Dennis L Kasper; Fiscal Year: 2013
    ..In the proposed studies, we will determine whether CD4+ T cells can differentiate glycopeptides from peptides and whether glycopeptide epitopes can induce IgM-to-IgG switching...
  8. Glycoconjugate therapeutic peptides for improved treatment of human diseases
    ADAM CHARLES FISHER; Fiscal Year: 2013
    ..to this growing, unsolved problem by engineering bacteria as a platform for the biosynthesis of therapeutic glycopeptides. These novel strains of Escherichia coli are useful for the expression of recombinant peptides conjugated to ..
  9. T CELL IMMUNITY TO EPITHELIAL TUMOR MUCINS
    Olivera J Finn; Fiscal Year: 2013
    ..We will use vaccines based on immunogenic MUC1 glycopeptides defined in the last grant period as well as MUC1 specific TCR transgenic mice developed in the same period...
  10. Synthesis of MUC1 glycopeptides and generation of antibodies for the indentificat
    Katja Michael; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Synthesis of MUC1 glycopeptides and generation of antibodies for the identification of epitopes on cancer cells...
  11. Protective Glycopeptide Immunodeterminants of Ehrlichia chaffeensis
    Jere W McBride; Fiscal Year: 2010
    ..chaffeensis are defined by O-linked hexose glycopeptides within major immunoreactive glycoproteins, including gp47...
  12. The role of N-glycosylation on beta-sheet protein folding energetics
    JOSHUA L PRICE; Fiscal Year: 2010
    ..of the ability of these thiol-functionalized Asn-linked sugars to facilitate the chemical ligation of short glycopeptides to peptide thioesters at ligation junctions that would be useful for full-length CD2ad synthesis;(3) ..
  13. Smaller Particle Fused-Core Silicas for Higher Performance Separations
    Barry E Boyes; Fiscal Year: 2011
    ..chromatographic (HPLC) separations for both small molecules and larger molecules such as proteins, peptides, glycopeptides, and glycans...
  14. Novel Materials and Methods for Separations of Glycopeptides and Glycans
    Barry E Boyes; Fiscal Year: 2011
    ..Hydrophilic interaction liquid chromatography (HILIC) has great use in separations of glycopeptides and released glycans, exhibiting remarkable selectivity for the separations of glycoforms...
  15. SYNTHESIS OF ANTITUMOR NATURAL PRODUCTS
    Samuel J Danishefsky; Fiscal Year: 2013
    ..The capacity to synthesize peptides, glycopeptides, proteins, and glycoproteins in homogeneous form will provide important opportunities...
  16. BIOSYNTHESIS OF COMPLEX CARBOHYDRATES IN BRAIN
    LENNART RODEN; Fiscal Year: 1993
    ..Xylose-containing glycopeptides with alternating serine and glycine residues and two compounds with phosphorylated xylose will also be ..
  17. NCL with induced a-Helices: de Novo Chemical Synthesis of the ATAD2 bromodomain
    GARDNER SILAS CREECH; Fiscal Year: 2013
    ..Currently, however, chemical synthesis of proteins is limited to relatively small sized polypeptides and glycopeptides, due to a number of practical factors...
  18. Glycoprotein biomarkers for the early detection of aggressive prostate cancer
    Hui Zhang; Fiscal Year: 2013
    ..Aim 2, we will develop highly sensitive, specific, and high throughput MS based SRM assays for the candidate glycopeptides identified from Aim 1...
  19. Convergent Chemoenzymatic Synthesis of Glycopeptides and Glycoproteins
    Lai Xi Wang; Fiscal Year: 2013
    ..research is to develop a general and highly efficient chemo enzymatic method for making diverse homogeneous glycopeptides and glycoproteins of biomedical significance...
  20. Novel Materials and Methods for Separations of Glycopeptides and Glycans
    Barry E Boyes; Fiscal Year: 2013
    ..Hydrophilic interaction liquid chromatography (HILIC) has great use in separations of glycopeptides and released glycans, exhibiting remarkable selectivity for the separations of glycoforms...
  21. Acquisition of Microwave-Assisted Automated Peptide Synthesizer
    David H Live; Fiscal Year: 2010
    ..Synthetic glycopeptides provide powerful alternative agents for investigating many aspects of glycoproteins...
  22. Glycan Markers for the Early Detection of Breast Cancer
    William S Hancock; Fiscal Year: 2011
    ..point of the glycan to the polypeptide backbone and the accurate mass and fragmentation pattern of individual glycopeptides in the LT-FTMS and LTMS with both CID and ETD will be determined...
  23. SBIR TOPIC 294 PHASE 1 - DEVELOPMENT OF GLYCOSYLATION - SPECIFIC RESEARCH
    Bill Jackson; Fiscal Year: 2011
    ..We will also develop high affinity reagents to specific glycopeptides which are likely to find enormous utility in the understanding of cancer glycobiology.
  24. Molecular Recognition by Bleomycin
    Sidney M Hecht; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The bleomycins are a family of glycopeptides derived antitumor antibiotics originally isolated from Streptomyces verticillus...
  25. New Reactions for Direct, Native Peptide Ligations
    JEFFREY WILLIAM BODE; Fiscal Year: 2010
    ..These studies will provide new methods for the synthesis of biomolecule targets including proteins, glycopeptides, and peptidomimetics...
  26. Synthetic Variable Domain Glycopeptides for Neutralizing Epitope Characterization
    Lai Xi Wang; Fiscal Year: 2013
    ..We hypothesize that unique V1/V2 and V3 glycopeptides constitute the neutralizing epitopes for these bNAbs...
  27. ORGANIC NANOTUBES DESIGN AND BIOLOGICAL APPLICATIONS
    M Ghadiri; Fiscal Year: 2009
    ..from the design of novel heterocyclic a,e-and a,(3-cyclic peptide nanotubes, metal ion-dependent systems, glycopeptides, and prodrug concepts to rational and combinatorial library approaches directed at the design and discovery of ..
  28. MYCOBACTERIAL CELL WALL AND DRUG DISCOVERY
    Patrick Brennan; Fiscal Year: 2007
    ..with a probing of their biosynthesis,[unreadable] based on observations that they originate in water-soluble glycopeptides[unreadable] and are susceptible to D cycloserine...
  29. Ellipsoid Particles for High Performance LC Separations
    Barry E Boyes; Fiscal Year: 2013
    ..chromatographic (HPLC) separations for both small molecules and larger molecules such as proteins, peptides, glycopeptides, and glycans...
  30. CARBOHYDRATE-CONTAINING MACROMOLECULES IN AGING BRAIN
    ERIC BRUNNGRABER; Fiscal Year: 1980
    ..The defatted residue is treated with papain to solubilize glycopeptides and glycosaminoglycans...
  31. ROLE OF THYROGLOBULIN IN THYROXINE BIOSYNTHESIS
    ALLEN RAWITCH; Fiscal Year: 1990
    ..Tryptic glycopeptides will be isolated and sequenced in order to define the sites of glycosylation with the primary structure ..
  32. Development of a rapid glycosylation profiling system using mass spec data
    HEATHER R DESAIRE; Fiscal Year: 2012
    The ultimate goal of this proposal is to develop a fully-automated approach for analysis of glycopeptides and to apply that approach to the analysis of several different HIV-Env glycoproteins...
  33. IgA-secreting B cell lines: a novel tool for studies of IgA nephropathy
    Jan Novak; Fiscal Year: 2009
    ..greatly by our development of high throughput methods of analysis, state-of-the-art techniques for analysis of glycopeptides, and our extensive basic and clinical collaborations that permit testing of the hypothesis by experimental ..
  34. STRUCTURES OF NEURECTODERM TUMOR GLYCOPROTEINS
    MARY GLICK; Fiscal Year: 1992
    ..We will continue to study the glycopeptides from NIH 3T3 and alpha 1-1 cells by lectin affinity and NMR analysis...
  35. Novel Glycosylated Delta Opioid Receptor Agonists for Chronic Inflammatory Pain
    KIMBERLEY GANNON; Fiscal Year: 2009
    ..We intend to synthesize 15-20 novel glycopeptides from the deltorphin scaffold (Specific Aim 1) and screen the compounds for in vitro binding, selectivity and ..
  36. CHARACTERIZATION OF MICROBIAL LECTINS
    Patricia Murray; Fiscal Year: 1992
    ..We have prepared a large number of well-characterized glycopeptides as structural analogues of salivary glycoproteins...
  37. Accurate profiles of IgA1 O-glycan heterogeneity in IgA nephropathy
    MATTHEW RENFROW; Fiscal Year: 2009
    ..Thus, a method is needed that can provide detailed structural information about the population of O-glycopeptides within each sample...
  38. SBIR TOPIC321:CHEMICALLY DEFINED GLYCAN LIBRARIES FOR REFERENCE STANDARDS AND GLY
    Jian Zhang; Fiscal Year: 2013
    ..David Live at CCRC in Phase I to assemble the glycosyl building blocks to glycopeptides for proof-of-concept demonstration...
  39. CARBOHYDRATE FRACTIONATION FACILITY
    Pamela Stanley; Fiscal Year: 1990
    ..techniques for rapidly separating and quantitating complex mixtures of monosaccharides, oligosaccharides and glycopeptides with maximal resolution and sensitivity...
  40. GLYCOPEPTIDE RESISTANCE LOCI IN STAPHYLOCOCCUS AUREUS
    Susan Boyle Vavra; Fiscal Year: 2001
    DESCRIPTION (Adapted from the applicant's abstract): The glycopeptides (GP) vancomycin (Vm) and teicoplanin (Tco) are the agents of choice in treating infections caused by methicillin-resistant staphylococci which are often multiply ..
  41. MECHANISMS OF GLYCOPEPTIDE RESISTANCE IN STAPHYLOCOCCI
    Robert Daum; Fiscal Year: 2006
    ..The glycopeptides (GP) have been the most reliable alternatives for the therapy of S...
  42. CHEMISTRY OF INHIBIN AND RELATED HORMONES
    DARRELL WARD; Fiscal Year: 1990
    ..For these two studies we will use selective protein degradation methods to isolate the glycopeptides and disulfide-containing peptides, respectively...
  43. Sulfhydryl Cross-linked Targeted Gene Delivery
    KEVIN RICE; Fiscal Year: 2006
    ..specificity will be achieved by derivatizing a cross-linking peptide with a single N-glycan resulting in glycopeptides that direct targeting to either the asialoglycoprotein receptor on hepatocytes or the mannose receptor on ..
  44. CHARACTERIZATION OF HUMAN CARCINOMA T & TN AUTOANTIGENS
    GEORG SPRINGER; Fiscal Year: 1991
    ..Purification of T- & Tn-active glycopeptides obtained by pronase digestion and their reductively beta-eliminated oligosaccharides (OS) by gel filtration, ..
  45. ABI QSTAR XL Q-Q TOF Mass Spectrometer
    BRADLEY HAYES; Fiscal Year: 2004
    ..an ABI QSTAR XL QqTOF mass spectrometer and MALDI source for the structural characterization of glycans and glycopeptides. This instrument will support the research aims of a diverse group of well-funded investigators with a common ..
  46. EXOGENOUS SUBSTRATES FOR GLYCOPROTEIN BIOSYNTHESIS
    Christopher Warren; Fiscal Year: 1992
    ..this proposal, the influence of peptide structure on processing will be explored by the chemical synthesis of glycopeptides with different peptide sequences adjacent to the glycosylation site...
  47. BIOORGANIC APPROACH TO CARBOHYDRATE RECOGNITION
    RANDALL HALCOMB; Fiscal Year: 2001
    ..These glycopeptides would be evaluated as inhibitors or substrates of the Core 1 galactosyltransferase, and enzyme that catalyzes ..
  48. Profile serum proteins by glycopeptide capture and LC-MS
    Hui Zhang; Fiscal Year: 2006
    ..isolation of the peptides that are N-linked glycosylated in the intact protein using solid-phase extraction of glycopeptides (SPEG) on a robotic workstation, the analysis of these now de-glycosylated peptides by liquid chromatography ..
  49. ANTITUMOR ACTIVE COMPONENTS OF BCG CELL WALLS
    Gary Gray; Fiscal Year: 1993
    ..underlying our work is that the macrophage would phagocytize and catabolize an invading bacterium, releasing glycopeptides unique to bacteria to serve as signals for immune amplification...
  50. TAU GLYCOSYLATION IN ALZHEIMERS DISEASE
    Cheng Xin Gong; Fiscal Year: 2001
    ..The glycoylation sites will be mapped by generation and isolation of glycopeptides, and their amino acid sequencing and matrix-assisted laser desorption/ionization-Mass spectrometry...
  51. Biochemical Role of Airway Mucins in Cystic Fibrosis
    GOVERDHAN SACHDEV; Fiscal Year: 2003
    ..aeruginosa by preparing glycopeptides and individual saccharides from CF and control mucins...
  52. 500 MHZ NMR INSTRUMENTATION FOR SHARED USE
    Craig Townsend; Fiscal Year: 1991
    ..2) Synthetic and structural investigations of oligosaccarides, glycopeptides and glycolipids for research in carbohydrate recognition and hepatic receptor binding studies...
  53. A proteomic approach for early diagnosis of diabetes
    Julian Watts; Fiscal Year: 2005
    ..quantitative plasma protein profiling, based upon our recently developed method for solid-phase extraction of glycopeptides (SPEG) and peptide detection and identification by mass spectrometry (MS); 2) the application of software ..
  54. ENHANCED B VIRUS DIAGNOSIS/CONTROL IN PRIMATE COLONIES
    Julia Hilliard; Fiscal Year: 2000
    ..Specific Aims: (1) identify glycoproteins and proteins from which each of the infected cell polypeptides/glycopeptides are derived; (2) sequence major gene homologues encoded by B virus and identify divergent and conserved ..
  55. SYNTHESIS, CONFORMATION AND APPLICATION OF GLYCOPEPTIDES
    Laszlo Otvos; Fiscal Year: 2000
    ..In the first tow cycles of this grant, we have developed new methodologies for the solid-phase synthesis of glycopeptides and have established the principle effects glycosylation exerts on the conformational, in vitro stability and ..
  56. GLYCOPEPTIDES AS TARGETED DRUG DELIVERY CARRIERS
    KEVIN RICE; Fiscal Year: 2000
    ..The aim of this proposal is to optimize low molecular weight glycopeptides for hepatic gene delivery...
  57. Translational and Clinical Research on Cancer: Glycomics Applications
    James Pierce; Fiscal Year: 2009
    ..Japan and the United States have developed new technologies that allow a comprehensive analysis of glycans and glycopeptides from diseased human tissues and fluids as a means to identify potential cancer and other disease markers...
  58. STRUCTURE AND FUNCTION OF VIRAL GLYCOPEPTIDES
    Lawrence Hunt; Fiscal Year: 1980
    ..The affects of viral transformation, virus infection and cell growth on the oligosaccharide biosynthetic machinery will also be addressed...
  59. Fluorous Solution-Phase Synthesis of Peptides
    Nicola Pohl; Fiscal Year: 2005
    Ready access to various oligosaccharides and glycopeptides is crucial for the development of glycobiology and glycomics-the system-wide study of the interactions of oligosaccharides with other biomolecules such as proteins...
  60. LC-ELECTROSPRAY QUADRUPOLE ION TRAP MASS SPECTROMETER
    Dominic Desiderio; Fiscal Year: 2000
    ..Structures of biologically active compounds: (i) bacterial cell wall constituents (lipopolysaccharides and glycopeptides) involved in macrophage activation; and (ii) blood-derived phospholipid growth factors...
  61. SURFACE GLYCOPEPTIDES, GROWTH REGULATION IN ANIMAL CELLS
    Costante Ceccarini; Fiscal Year: 1980
    ..Surface glycopeptides from a variety of cells will be labeled with radioactive carbohydrate precursors and after brief proteolysis, ..
  62. A New Platform to Screen Serum for Cancer Membrane Proteins
    Daniel Martin; Fiscal Year: 2007
    ..glycoproteins from four diverse culture models of human prostate cancer to generate an ensemble of target glycopeptides. To enable quantification, we generate isotopically heavy versions of these peptides by concatenating the DMA ..
  63. MALDI TOF/TOF TANDEM MASS SPECTROMETER
    Alma Burlingame; Fiscal Year: 2000
    ..with high resolution and high mass accuracy for-high energy CID sequencing of biomolecules such as peptides, glycopeptides and oligosaccharides to project investigators with established needs...
  64. LC/MS/MS Purchase
    Robert Hodges; Fiscal Year: 2003
    ..identification of cell surface proteins through selection and structural determination of their tryptic glycopeptides. (B. Bendiak) Structure and mechanism in coordinated gene regulation. (M...