interleukin 3

Summary

Summary: A multilineage cell growth factor secreted by lymphocytes, epithelial cells, and astrocytes which stimulates clonal proliferation and differentiation of various types of blood and tissue cells. Also called multi-CSF, it is considered one of the hematopoietic colony stimulating factors.

Top Publications

  1. ncbi Role for interleukin-3 in mast-cell and basophil development and in immunity to parasites
    C S Lantz
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 392:90-3. 1998
  2. pmc Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes
    S Ikushima
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 94:2609-14. 1997
  3. ncbi Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL
    R Salgia
    Division of Hematologic Malignancies, Dana Farber Cancer Institute, Boston, Massachusetts 02115
    J Biol Chem 270:5039-47. 1995
  4. ncbi Regulation of mast cell survival by IgE
    K Asai
    Division of Allergy, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Immunity 14:791-800. 2001
  5. pmc Trophic factor withdrawal: p38 mitogen-activated protein kinase activates NHE1, which induces intracellular alkalinization
    A R Khaled
    Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA
    Mol Cell Biol 21:7545-57. 2001
  6. pmc Differential expression of secretory granule proteases in mouse mast cells exposed to interleukin 3 and c-kit ligand
    M F Gurish
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    J Exp Med 175:1003-12. 1992
  7. pmc Interleukin 3-dependent activation of DREAM is involved in transcriptional silencing of the apoptotic Hrk gene in hematopoietic progenitor cells
    C Sanz
    Unidad de Genetica Molecular, Hospital Universitario Marques de Valdecilla, 39008 Santander, Spain
    EMBO J 20:2286-92. 2001
  8. ncbi Erythropoietin receptor haploinsufficiency and in vivo interplay with granulocyte-macrophage colony-stimulating factor and interleukin 3
    Armin G Jegalian
    Department of Molecular and Medical Pharmacology, Molecular Biology Institute, University of California Los Angeles School of Medicine, Los Angeles, CA 90095 1735, USA
    Blood 99:2603-5. 2002
  9. ncbi MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors
    Brian J P Huntly
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Cancer Cell 6:587-96. 2004
  10. ncbi Induction of apoptosis by a secreted lipocalin that is transcriptionally regulated by IL-3 deprivation
    L R Devireddy
    Howard Hughes Medical Institute, Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Science 293:829-34. 2001

Detail Information

Publications309 found, 100 shown here

  1. ncbi Role for interleukin-3 in mast-cell and basophil development and in immunity to parasites
    C S Lantz
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 392:90-3. 1998
    ..These findings establish a role for IL-3 in immunity to parasites and indicate that one of the functions of IL-3 in host defence against infection is to expand populations of haematopoietic effector cells...
  2. pmc Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes
    S Ikushima
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 94:2609-14. 1997
    ..When introduced into interleukin 3 (IL-3)-dependent mouse pro-B lymphocytes, E2A-HLF prevents apoptosis induced by growth factor deprivation, ..
  3. ncbi Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL
    R Salgia
    Division of Hematologic Malignancies, Dana Farber Cancer Institute, Boston, Massachusetts 02115
    J Biol Chem 270:5039-47. 1995
    ..The predicted structure of paxillin implicates this molecule in protein-protein interactions involved in signal transduction from growth factor receptors and the BCR/ABL oncogene fusion protein to the cytoskeleton...
  4. ncbi Regulation of mast cell survival by IgE
    K Asai
    Division of Allergy, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Immunity 14:791-800. 2001
    ..The ability of IgE to enhance mast cell survival and Fc epsilon RI expression may contribute to amplified allergic reactions...
  5. pmc Trophic factor withdrawal: p38 mitogen-activated protein kinase activates NHE1, which induces intracellular alkalinization
    A R Khaled
    Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA
    Mol Cell Biol 21:7545-57. 2001
    ..Thus, loss of trophic cytokine signaling induced the p38 MAPK pathway, which phosphorylated NHE1 at specific sites, inducing intracellular alkalinization...
  6. pmc Differential expression of secretory granule proteases in mouse mast cells exposed to interleukin 3 and c-kit ligand
    M F Gurish
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    J Exp Med 175:1003-12. 1992
    ..When bone marrow-derived MC (BMMC) were developed with recombinant interleukin 3 (rIL-3) and maintained with this cytokine in the absence or presence of recombinant c-kit ligand (rKL), they ..
  7. pmc Interleukin 3-dependent activation of DREAM is involved in transcriptional silencing of the apoptotic Hrk gene in hematopoietic progenitor cells
    C Sanz
    Unidad de Genetica Molecular, Hospital Universitario Marques de Valdecilla, 39008 Santander, Spain
    EMBO J 20:2286-92. 2001
    ....
  8. ncbi Erythropoietin receptor haploinsufficiency and in vivo interplay with granulocyte-macrophage colony-stimulating factor and interleukin 3
    Armin G Jegalian
    Department of Molecular and Medical Pharmacology, Molecular Biology Institute, University of California Los Angeles School of Medicine, Los Angeles, CA 90095 1735, USA
    Blood 99:2603-5. 2002
    ..To evaluate the interaction between EPO and granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin 3 (IL-3), GM-CSF(-/-) or IL-3(-/-) mice were interbred with EpoR(+/)(-) mice...
  9. ncbi MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors
    Brian J P Huntly
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Cancer Cell 6:587-96. 2004
    ..These data demonstrate that some, but not all, leukemia oncogenes can confer properties of leukemic stem cells to hematopoietic progenitors destined to undergo apoptotic cell death...
  10. ncbi Induction of apoptosis by a secreted lipocalin that is transcriptionally regulated by IL-3 deprivation
    L R Devireddy
    Howard Hughes Medical Institute, Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Science 293:829-34. 2001
    ..We conclude that IL-3 deprivation activates 24p3 transcription, leading to synthesis and secretion of 24p3, which induces apoptosis through an autocrine pathway...
  11. ncbi Biology of common beta receptor-signaling cytokines: IL-3, IL-5, and GM-CSF
    Margarita Martinez-Moczygemba
    Biology of Inflammation Center, Departments of Medicine and Immunology, Baylor College of Medicine, Houston, TX, USA
    J Allergy Clin Immunol 112:653-65; quiz 666. 2003
    ..Delineating the biology of these cytokines is enabling the development of new strategies for diagnosing and treating these diseases and modulating immune responses...
  12. ncbi Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt
    L del Peso
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Science 278:687-9. 1997
    ..Thus, the proapoptotic function of BAD is regulated by the PI 3-kinase-Akt pathway...
  13. pmc Human thymus contains IFN-alpha-producing CD11c(-), myeloid CD11c(+), and mature interdigitating dendritic cells
    N Bendriss-Vermare
    Schering-Plough, Laboratory for Immunological Research, Dardilly, France
    J Clin Invest 107:835-44. 2001
    ..The detection of Spi-B mRNA, not only upon in vitro maturation of pDCs, but also in freshly purified IDCs, suggests that in vivo pDCs may differentiate into IDCs...
  14. ncbi The role of c-Kit and its ligand, stem cell factor, in mast cell apoptosis
    Y A Mekori
    Department of Medicine, Meir General Hospital, Tel Aviv, Israel
    Int Arch Allergy Immunol 107:136-8. 1995
    ..Thus, microenvironmental factors play an important role in regulating mast cell numbers by effecting survival in the periphery...
  15. ncbi Primitive interleukin 3 null hematopoietic cells transduced with BCR-ABL show accelerated loss after culture of factor-independence in vitro and leukemogenic activity in vivo
    Xiaoyan Jiang
    Terry Fox Laboratory, British Columbia Cancer Agency, and the Department of Medicine, Laboratory Medicine, and Medical Genetics, University of British Columbia, Vancouver, BC, Canada
    Blood 100:3731-40. 2002
    Primitive chronic myeloid leukemia cells display a unique autocrine interleukin 3 (IL-3)/granulocyte-colony-stimluating factor (G-CSF) mechanism that may explain their abnormal proliferation and differentiation control...
  16. ncbi IL-6 precludes the differentiation induced by IL-3 on expansion of CD34+ cells from cord blood
    Araceli Encabo
    Instituto de Biologia Celular, Organismo Público Valenciano de Investigación, Valencia, Spain
    Haematologica 88:388-95. 2003
    ....
  17. pmc Transgenic mice for interleukin 3 develop motor neuron degeneration associated with autoimmune reaction against spinal cord motor neurons
    C Chavany
    Genetics and Molecular Immunology Section, Laboratory of Immunology National Eye Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda MD 20892, USA
    Proc Natl Acad Sci U S A 95:11354-9. 1998
    b>Interleukin 3 (IL-3) stimulates the proliferation and differentiation of various haematopoietic progenitor cells...
  18. pmc Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 transduce signals through two STAT5 homologs
    A L Mui
    Department of Cell Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304 1104, USA
    EMBO J 14:1166-75. 1995
    ..In IL-3-dependent hemopoietic cells, both forms of STAT5 are expressed and activated in response to IL-3...
  19. pmc Distinct methylation of the interferon gamma (IFN-gamma) and interleukin 3 (IL-3) genes in newly activated primary CD8+ T lymphocytes: regional IFN-gamma promoter demethylation and mRNA expression are heritable in CD44(high)CD8+ T cells
    D R Fitzpatrick
    Leukocyte Biology Unit, The Queensland Institute of Medical Research, Post Office Royal Brisbane Hospital, Queensland 4029, Australia
    J Exp Med 188:103-17. 1998
    ....
  20. ncbi Requirement for Mdm2 in the survival effects of Bcr-Abl and interleukin 3 in hematopoietic cells
    A W Goetz
    Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology, Stuttgart, Germany
    Cancer Res 61:7635-41. 2001
    ..These findings imply that, similarly to physiological growth factors such as IL-3, Bcr-Abl can promote cell survival through modulating the p53-Mdm2 pathway...
  21. ncbi Cultured human intestinal mast cells express functional IL-3 receptors and respond to IL-3 by enhancing growth and IgE receptor-dependent mediator release
    Thomas Gebhardt
    Department of Gastroenterology, Hepatology and Endocrinology, Medical School of Hannover, Hannover, Germany
    Eur J Immunol 32:2308-16. 2002
    ..In conclusion, our data show that human intestinal mast cells express functional IL-3R, indicating that IL-3 not only regulates growth and function of immature, but also that of mature human mast cells...
  22. ncbi Interleukin-3 withdrawal induces an early increase in mitochondrial membrane potential unrelated to the Bcl-2 family. Roles of intracellular pH, ADP transport, and F(0)F(1)-ATPase
    A R Khaled
    Laboratory of Molecular Immunoregulation, Division of Basic Sciences, NCI, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 276:6453-62. 2001
    ....
  23. ncbi Stimulation of mouse bone marrow cells with kit ligand, FLT3 ligand, and thrombopoietin leads to efficient retrovirus-mediated gene transfer to stem cells, whereas interleukin 3 and interleukin 11 reduce transduction of short- and long-term repopulating c
    A W Wognum
    Institute of Hematology, Erasmus Universiteit, 3000 DR Rotterdam, The Netherlands
    Hum Gene Ther 11:2129-41. 2000
    ..cells preconditioned with various combinations of Kit ligand (KL), FLT-3 ligand (FL), thrombopoietin (TPO), interleukin 3 (IL-3), and IL-11...
  24. ncbi Transcriptional regulation of the mucosal mast cell-specific protease gene, MMCP-2, by interleukin 10 and interleukin 3
    N Ghildyal
    Department of Medicine, Harvard Medical School, Brigham and Women s Hospital, Boston, Massachusetts 02115
    J Biol Chem 267:8473-7. 1992
    ....
  25. pmc Interleukin 3 stimulates protein synthesis by regulating double-stranded RNA-dependent protein kinase
    T Ito
    Johns Hopkins Oncology Center, Baltimore, MD 21231
    Proc Natl Acad Sci U S A 91:7455-9. 1994
    In a murine interleukin 3 (IL-3)-dependent cell line, IL-3 deprivation resulted in increased autophosphorylation of double-stranded RNA-dependent protein kinase (PKR) that has been reported to inhibit protein synthesis by phosphorylating ..
  26. ncbi Inhibitory effect of interleukin 3 on early development of human B-lymphopoiesis
    K Miyamoto
    Department of Clinical Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Br J Haematol 114:690-7. 2001
    b>Interleukin 3 (IL-3) is known as a stimulator of proliferation and differentiation of non-lymphoid cells, but information about the activity of IL-3 in lymphopoiesis is limited...
  27. ncbi Cytokine receptor repertoire and cytokine responsiveness of Ho(dull)/Rh(dull) stem cells with differing potentials for G1/S phase progression
    G Prem Veer Reddy
    Vattikuti Urology Institute, Henry Ford Health Sciences Center, Detroit, MI, USA
    Exp Hematol 30:792-800. 2002
    ....
  28. ncbi Homing of purified murine lymphohematopoietic stem cells: a cytokine-induced defect
    Jan Cerny
    Department of Research, Roger Williams Medical Center, Providence, RI 02908, USA
    J Hematother Stem Cell Res 11:913-22. 2002
    ..Exposure of marrow stem cells to IL-3, IL-6, IL-11, and steel factor induces a stem cell homing defect, which probably underlies the engraftment defect previously characterized under these conditions...
  29. doi Interleukin 3 and schizophrenia
    Xiangning Chen
    UT Southwestern Medical Center, Department of Psychiatry, 5323 Harry Hines Blvd, NE5 110, Dallas, TX 75390 9070, USA
    Am J Psychiatry 165:13-4. 2008
  30. pmc Interleukin 3-dependent mediator release in basophils triggered by C5a
    Y Kurimoto
    Institute of Clinical Immunology, Inselspital, Bern, Switzerland
    J Exp Med 170:467-79. 1989
    ....
  31. ncbi Akt activation promotes degradation of tuberin and FOXO3a via the proteasome
    David R Plas
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylania 19104, USA
    J Biol Chem 278:12361-6. 2003
    ..Together the data indicate that activated Akt stimulates proteasomal degradation of its substrates and suggest that Akt-dependent cell growth and survival are induced through the degradation of negative regulators of these processes...
  32. ncbi Interleukin 3 (IL3) polymorphisms associated with decreased risk of asthma and atopy
    Byung Lae Park
    Department of Genetic Epidemiology, SNP Genetics, Inc, 11th Floor, MaeHun B D, 13 Chongro 4 Ga, Chongro Gu, Seoul, 110 834, South Korea
    J Hum Genet 49:517-27. 2004
    ..007). This information about the genetic association of important genes with asthma might provide valuable insights into strategies for the pathogenesis of asthma and atopy...
  33. pmc Mitogenic signalling and substrate specificity of the Flk2/Flt3 receptor tyrosine kinase in fibroblasts and interleukin 3-dependent hematopoietic cells
    M Dosil
    Department of Molecular Biology, Princeton University, New Jersey 08544 1014
    Mol Cell Biol 13:6572-85. 1993
    ..In transfected interleukin 3 (IL-3)-dependent Ba/F3 lymphoid cells, activation of the chimeric receptor can abrogate IL-3 requirement and ..
  34. ncbi Death and Baxes: mechanisms of lymphotrophic cytokines
    Annette R Khaled
    Biomolecular Science Center, Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL, USA
    Immunol Rev 193:48-57. 2003
    ..The IL-7 survival and death pathways in lymphocytes may be representative of many trophic factors in different cell types; yet we conclude that much of the mechanism remains to be discovered...
  35. ncbi A novel function for transforming growth factor-beta1: upregulation of the expression and the IgE-independent extracellular release of a mucosal mast cell granule-specific beta-chymase, mouse mast cell protease-1
    H R Miller
    Department of Veterinary Clinical Studies, Royal Dick School of Veterinary Studies, The University of Edinburgh, Easter Bush Veterinary Centre, Easter Bush, Roslin, Midlothian, Scotland
    Blood 93:3473-86. 1999
    ..In conclusion, the expression and extracellular release of the IMMC-specific chymase, mMCP-1, is strictly regulated by TGF-beta1...
  36. pmc Human peripheral blood basophils primed by interleukin 3 (IL-3) produce IL-4 in response to immunoglobulin E receptor stimulation
    T Brunner
    Institute of Clinical Immunology, Bern, Switzerland
    J Exp Med 177:605-11. 1993
    ....
  37. ncbi Stat5 expression is critical for mast cell development and survival
    Christopher P Shelburne
    Department of Biology, Virginia Commonwealth University, Richmond, VA 23284 2012, USA
    Blood 102:1290-7. 2003
    ..These results indicate that Stat5A and Stat5B are critical regulators of in vitro and in vivo mast cell development and survival...
  38. ncbi BCR-ABL mutants spread resistance to non-mutated cells through a paracrine mechanism
    J Liu
    INSERM, Unité 837, Equipe 3, Institut de Recherche sur le Cancer de Lille, Lille, France
    Leukemia 22:791-9. 2008
    ..In co-culture, mutated cells were able to spread resistance to non-mutated cells through overexpression of interleukin 3, activation of MEK/ERK and JAK2/STAT5 pathways, and downregulation of Bim...
  39. pmc Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein
    G Q Daley
    Whitehead Institute for Biomedical Research, Cambridge Center, MA 02142
    Proc Natl Acad Sci U S A 85:9312-6. 1988
    ..cell types, retroviral constructs encoding P210bcr/abl were used to infect the bone marrow-derived interleukin 3-dependent Ba/F3 cell line...
  40. ncbi Interleukin 3 and granulocyte-macrophage colony-stimulating factor are not required for induction of chronic myeloid leukemia-like myeloproliferative disease in mice by BCR/ABL
    S Li
    The Center for Blood Research, Dana-Farber Cancer Institute, and Harvard Institutes of Medicine and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA
    Blood 97:1442-50. 2001
    ..with Philadelphia chromosome (Ph)-positive chronic myeloid leukemia (CML) express aberrant transcripts for interleukin 3 (IL-3) and granulocyte colony-stimulating factor (G-CSF), and exhibit autonomous proliferation in serum-free ..
  41. pmc Conditional immortalization of mouse myelomonocytic, megakaryocytic and mast cell progenitors by the Hox-2.4 homeobox gene
    A C Perkins
    Walter and Eliza Hall Institute of Medical Research, Australia
    EMBO J 12:3835-46. 1993
    ..Thus ectopic expression of Hox-2.4 enhances self-renewal of immature myeloid progenitors and progression to a fully malignant state is favoured by somatic mutations conferring autocrine production of IL-3...
  42. ncbi Polymorphisms in the interleukin 3 gene show strong association with susceptibility to Graves' disease in Chinese population
    X Chu
    Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China
    Genes Immun 10:260-6. 2009
    ..Initial analysis suggested that a non-synonymous SNP rs40401 (P27S) of interleukin 3 (IL3) was associated with GD, and further fine-mapping showed that rs40401, or its perfect proxy SNP rs31480 ..
  43. ncbi Interleukin-3 stimulates migration and proliferation of vascular smooth muscle cells: a potential role in atherogenesis
    M F Brizzi
    Department of Internal Medicine, University of Turin, Italy
    Circulation 103:549-54. 2001
    ....
  44. pmc Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line
    Y Weinstein
    Proc Natl Acad Sci U S A 83:5010-4. 1986
    ..were compared with those of other myeloid cell lines, and the known sequence of differentiation induced by interleukin 3. Similar to other myeloid cell lines, the NFS-60 cells do not terminally differentiate in response to ..
  45. ncbi The shc adaptor protein forms interdependent phosphotyrosine-mediated protein complexes in mast cells stimulated with interleukin 3
    L Velazquez
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Blood 96:132-8. 2000
    ..On stimulation of hematopoietic cells with interleukin 3 (IL-3), Shc becomes phosphorylated and may therefore contribute to IL-3 signaling...
  46. pmc Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3
    M E Rothenberg
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    J Clin Invest 81:1986-92. 1988
    ..These phenotypic changes in the eosinophil may be advantageous to host defense against helminthic infections but may be disadvantageous in conditions such as allergic disease...
  47. ncbi CIS, a cytokine inducible SH2 protein, is a target of the JAK-STAT5 pathway and modulates STAT5 activation
    A Matsumoto
    Insititute of Life Science, Kurume University, Japan
    Blood 89:3148-54. 1997
    ..Thus, CIS is a feedback modulator of STAT5; its expression is induced by STAT5 and it negatively modulates STAT5 activation...
  48. ncbi A diphtheria toxin-interleukin 3 fusion protein is cytotoxic to primitive acute myeloid leukemia progenitors but spares normal progenitors
    Michaela Feuring-Buske
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, V5Z 1L3 Canada
    Cancer Res 62:1730-6. 2002
    The relative cytotoxicity of a diphtheria toxin (DT) human interleukin 3(IL3) fusion protein (DT(388)IL3) was tested against primitive normal (n = 3)and acute myeloid leukemia (AML) progenitors (n = 7)...
  49. ncbi Stat5B shuttles between cytoplasm and nucleus in a cytokine-dependent and -independent manner
    Rong Zeng
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Immunol 168:4567-75. 2002
    ....
  50. pmc Interleukin 3-dependent survival by the Akt protein kinase
    Z Songyang
    Department of Biology, Massachusetts Institute of Technology, 68 380, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 94:11345-50. 1997
    b>Interleukin 3 (IL-3)-dependent survival of hematopoietic cells is known to rely on the activity of multiple signaling pathways, including a pathway leading to activation of phosphoinositide 3-kinase (PI 3-kinase), and protein kinase Akt ..
  51. ncbi Differential expression and phosphorylation of distinct STAT3 proteins during granulocytic differentiation
    Diane L Hevehan
    Department of Chemical Engineering, Northwestern University, Evanston, IL 60208 3120, USA
    Blood 99:1627-37. 2002
    ....
  52. pmc Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction
    O Silvennoinen
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, TN 38105
    Proc Natl Acad Sci U S A 90:8429-33. 1993
    b>Interleukin 3 (IL-3) regulates the proliferation and differentiation of hematopoietic cells. Although the IL-3 receptor chains lack kinase catalytic domains, IL-3 induces tyrosine phosphorylation of cellular proteins...
  53. ncbi Stat5: an essential regulator of mast cell biology
    Christopher P Shelburne
    Department of Biology, Virginia Commonwealth University, Richmond, VA 23284 2012, USA
    Mol Immunol 38:1187-91. 2002
    ..These results indicate that Stat5 A and B are critical regulators of in vitro and in vivo mast cell biology...
  54. ncbi Cooperative and redundant effects of STAT5 and Ras signaling in BCR/ABL transformed hematopoietic cells
    R R Hoover
    Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
    Oncogene 20:5826-35. 2001
    ..These data illustrate both cooperative and redundant effects of STAT5 and Ras signaling in BCR/ABL transformed cells, with STAT5 playing a dominant role in resistance to DNA damage-induced apoptosis...
  55. pmc Interleukin-3, but not granulocyte-macrophage colony-stimulating factor and interleukin-5, inhibits apoptosis of human basophils through phosphatidylinositol 3-kinase: requirement of NF-kappaB-dependent and -independent pathways
    Xueyan Zheng
    iCAPTURE Center, McDonald Research Laboratory, University of British Columbia, St Paul s Hospital, 1081 Burrard Street, Vancouver, BC, Canada V6Z 1Y6
    Immunology 107:306-15. 2002
    ....
  56. pmc Mitochondrial respiratory control is lost during growth factor deprivation
    Eyal Gottlieb
    Abramson Family Cancer Research Institute, and Department of Cancer Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 99:12801-6. 2002
    ..Together, these data suggest that the regulation of mitochondrial outer membrane permeability contributes to respiratory control...
  57. ncbi Human umbilical vein endothelial cells support interleukin-3- and interleukin-5-induced eosinophil differentiation from cord blood CD34+ cells
    K Ochiai
    Department of Internal Medicine, Toho University School of Medicine, Sakura Hospital, Sakura, Japan
    Int Arch Allergy Immunol 120:2-6. 1999
    ..To show the supportive effects of endothelial cells on eosinophil differentiation, we examined the effects of cocultured HUVEC on IL-3 and IL-5-induced eosinophil differentiation from human umbilical cord blood CD34+ cells...
  58. ncbi Interleukin 3 and schizophrenia: the impact of sex and family history
    X Chen
    Department of Psychiatry and Virginia Institute for Psychiatric and Behavior Genetics, Virginia Commonwealth University, Richmond, VA 23298, USA
    Mol Psychiatry 12:273-82. 2007
    ..In this paper, we report evidence that multiple single nucleotide polymorphisms in and around interleukin 3 (IL3) are associated with the disease in the Irish Study of High-Density Schizophrenia Families (ISHDSF), the ..
  59. ncbi Differential regulation of human eosinophil IL-3, IL-5, and GM-CSF receptor alpha-chain expression by cytokines: IL-3, IL-5, and GM-CSF down-regulate IL-5 receptor alpha expression with loss of IL-5 responsiveness, but up-regulate IL-3 receptor alpha expr
    Bernard Gregory
    Department of Allergy and Clinical Immunology, National Heart and Lung Institute Division, Faculty of Medicine, Imperial College, London, United Kingdom
    J Immunol 170:5359-66. 2003
    ....
  60. ncbi A novel mechanism of tumor suppression by destabilizing AU-rich growth factor mRNA
    Georg Stoecklin
    Institute of Medical Microbiology, University of Basel, Petersplatz 10, CH 4003 Basel, Switzerland
    Oncogene 22:3554-61. 2003
    ....
  61. ncbi Characterization of the interactions between stromal and haematopoietic progenitor cells in expansion cell culture models
    N M Bilko
    Medical Centre of Tissue and Cell Therapy Embryotech, National University Kyiv Mohyla Academy, Kiev, Ukraine
    Cell Biol Int 29:83-6. 2005
    ....
  62. ncbi Neurotrophic action of interleukin 3 and granulocyte-macrophage colony-stimulating factor on murine sympathetic neurons
    Y Kannan
    Department of Veterinary Physiology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan
    Neuroimmunomodulation 8:132-41. 2000
    We investigated neurotrophic effects of interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on cultured sympathetic neurons obtained from mouse superior cervical ganglia...
  63. ncbi Convergence of signaling by interleukin-3, granulocyte-macrophage colony-stimulating factor, and mast cell growth factor on JAK2 tyrosine kinase
    M F Brizzi
    Dipartimento di Scienze Biomediche e Oncologia Umana, Universita di Torino, Italy
    J Biol Chem 269:31680-4. 1994
    ....
  64. ncbi A proapoptotic function of p21 in differentiating granulocytes
    Louis Ghanem
    University of Pittsburgh School of Medicine, Department of Medicine, Hillman Cancer Center, Lab 2 18, 5117 Center Avenue, Pittsburgh, PA 15213, USA
    Leuk Res 29:1315-23. 2005
    ..Our results also indicate there are distinct and opposing effects of p21 on monocytic and granulocytic survival. Aberrantly high levels of p21 may contribute to disease processes involving excessive apoptosis of granulocyte precursors...
  65. ncbi Association between nucleated red blood cells in blood and the levels of erythropoietin, interleukin 3, interleukin 6, and interleukin 12p70
    Axel Stachon
    Institute of Clinical Chemistry, Transfusion, and Laboratory Medicine, BG University Hospital Bergmannsheil, Ruhr University, D 44789 Bochum, Germany
    Shock 24:34-9. 2005
    ..Moreover, the increased number of reticulocytes in the blood of NRBC-positive patients may indicate that the appearance of NRBC is not associated with disturbed bone marrow function as far as the erythropoiesis is concerned...
  66. ncbi IL-3-mediated TNF production is necessary for mast cell development
    Harry V Wright
    Department of Biology, Virginia Commonwealth University, Richmond, 23284, USA
    J Immunol 176:2114-21. 2006
    ..Finally, TNF-deficient mice demonstrated a 50% reduction in peritoneal mast cell numbers, indicating that TNF is an important mast cell survival factor both in vitro and in vivo...
  67. ncbi p21Waf1 inhibits granulocytic differentiation of 32Dcl3 cells
    Louis Ghanem
    University of Pittsburgh School of Medicine, Department of Hematology Oncology, Pittsburgh, PA 15213, USA
    Leuk Res 30:1285-92. 2006
    ....
  68. ncbi Stimulation of human endothelium with IL-3 induces selective basophil accumulation in vitro
    Lina H K Lim
    Department of Medicine, Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    J Immunol 176:5346-53. 2006
    ..These data provide novel insights into how IL-3, acting directly on endothelium, can cause basophils to preferentially interact with blood vessels under physiological flow conditions and be selectively recruited to sites of inflammation...
  69. ncbi Transcription factors mediating interleukin-3 survival signals
    Jeffrey Jong Young Yen
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
    Vitam Horm 74:147-63. 2006
    ..The biological significance of the existence of these multiple survival-specific transcription pathways will also be discussed...
  70. ncbi Cytokines direct the regulation of Bim mRNA stability by heat-shock cognate protein 70
    Hirotaka Matsui
    Department of Molecular Oncology and Leukemia Program Project, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734 8553, Japan
    Mol Cell 25:99-112. 2007
    ..This unanticipated role of a chaperone/cochaperone complex in mRNA stability appears to be critical for hematopoiesis and leukemogenesis...
  71. ncbi A degradation-resistant c-Myb mutant cooperates with Bcl-2 in enhancing proliferative potential and survival of hematopoietic cells
    Francesca Corradini
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson Medical College, Philadelphia, PA, USA
    Blood Cells Mol Dis 39:292-6. 2007
    ....
  72. ncbi Phase I clinical study of diphtheria toxin-interleukin 3 fusion protein in patients with acute myeloid leukemia and myelodysplasia
    ARTHUR FRANKEL
    Scott and White Cancer Research Institute, Temple, TX, USA
    Leuk Lymphoma 49:543-53. 2008
    DT(388)IL3 fusion protein containing the catalytic and translocation domains of diphtheria toxin fused to human interleukin 3 was administered in an inter-patient dose escalation trial by 15 min i.v...
  73. ncbi Signal transducers and activators of transcription 3 augments the transcriptional activity of CCAAT/enhancer-binding protein alpha in granulocyte colony-stimulating factor signaling pathway
    Akihiko Numata
    Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, 812 8582, Japan
    J Biol Chem 280:12621-9. 2005
    ..Additionally, cooperation of C/EBPalpha with other Stat3-activated proteins are required for the induction of some G-CSF responsive genes including lysozyme M and the G-CSF receptor...
  74. ncbi Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification
    Erik A Nelson
    Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School and Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 279:54724-30. 2004
    ....
  75. ncbi IL-3-dependent murine mast cells undergo apoptosis on removal of IL-3. Prevention of apoptosis by c-kit ligand
    Y A Mekori
    Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
    J Immunol 151:3775-84. 1993
    ....
  76. ncbi The pims and outs of survival signaling: role for the Pim-2 protein kinase in the suppression of apoptosis by cytokines
    Eileen White
    Howard Hughes Medical Institute, Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, New Jersey 08854, USA
    Genes Dev 17:1813-6. 2003
  77. ncbi Regulation of survival pathways by IL-3 and induction of apoptosis following IL-3 withdrawal
    D E Johnson
    Department of Medicine, University of Pittsburgh, and the University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213 2582, USA
    Front Biosci 3:d313-24. 1998
    ..This review will discuss the current state of knowledge regarding the molecular mechanisms of: a) suppression of apoptosis by IL-3, and b) activation of apoptosis following IL-3 withdrawal...
  78. ncbi Transcriptional regulation of mouse granulocyte-macrophage colony-stimulating factor/IL-3 locus
    C S Osborne
    Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, Australia
    J Immunol 155:226-35. 1995
    ..Because the mouse GM-CSF enhancer is considerably less active than its human homologue we suggest that the mouse GM-CSF gene has evolved with less dependence upon the upstream enhancer for its activation...
  79. pmc Chromatin acetylation and remodeling at the Cis promoter during STAT5-induced transcription
    Anne Rascle
    Department of Discovery Research, DNAX Research Inc, 901 California Avenue, Palo Alto, CA 94304, USA
    Nucleic Acids Res 31:6882-90. 2003
    ..These data reveal novel implications of STAT5 in chromatin regulation during cytokine-induced transcription, thus contributing to a better understanding of the mechanism of transcriptional activation by STAT5...
  80. ncbi Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils
    P Wang
    Allergy Department, Schering Plough Research Institute, Kenilworth, NJ 07033, USA
    J Immunol 160:4427-32. 1998
    ..We suggest from these experiments that in human blood eosinophils, IL-5R alpha gene transcription and IL-5R alpha mRNA metabolism can be regulated by mechanisms that are distinct from those used for IL-3R alpha and GM-CSFR alpha...
  81. pmc gp130 and c-Kit signalings synergize for ex vivo expansion of human primitive hemopoietic progenitor cells
    X Sui
    Department of Clinical Oncology, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 92:2859-63. 1995
    ....
  82. ncbi Expansion of dendritic cell precursors from human CD34(+) progenitor cells isolated from healthy donor blood; growth factor combination determines proliferation rate and functional outcome
    Hetty J Bontkes
    Department of Pathology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands
    J Leukoc Biol 72:321-9. 2002
    ..Sequential cultures of both cocktails resulted in high numbers of potent APC, which can be useful DC-based cancer vaccines...
  83. ncbi Bcr-Abl efficiently induces a myeloproliferative disease and production of excess interleukin-3 and granulocyte-macrophage colony-stimulating factor in mice: a novel model for chronic myelogenous leukemia
    X Zhang
    Rosenstiel Basic Medical Sciences Research Center, The Department of Biochemistry, and the Department of Biology, Brandeis University, Waltham, MA, USA
    Blood 92:3829-40. 1998
    ..Our first use of this model for analysis of the molecular mechanisms involved in CML raises the possibility that excess expression of hematopoietic growth factors such as IL-3 and GM-CSF may contribute to the clinical phenotype of CML...
  84. ncbi Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A
    X Deng
    Sealy Center for Oncology and Hematology and Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555 1048, USA
    J Biol Chem 273:34157-63. 1998
    b>Interleukin 3 (IL-3) stimulates the net growth of murine factor-dependent NSF/N1.H7 and FDC-P1/ER myeloid cells by stimulating proliferation and suppressing apoptosis...
  85. ncbi Toxicology and pharmacokinetics of DT388IL3, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human interleukin 3 (IL3), in cynomolgus monkeys
    Kimberley A Cohen
    Department of Comparative Medicine and Cancer Biology, Wake Forest Health Sciences, Winston Salem, NC 27157, USA
    Leuk Lymphoma 45:1647-56. 2004
    ..Thus, we propose this agent is a promising drug for AML patients...
  86. pmc Interaction between interleukin 3 and dystrobrevin-binding protein 1 in schizophrenia
    Todd L Edwards
    Center for Human Genetics Research, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Schizophr Res 106:208-17. 2008
    ..We also observed a significant main effect in DTNBP1, which survived correction for multiple comparisons, and numerous nominally significant effects in several genes...
  87. ncbi Interleukin 3 improves the ex vivo expansion of primitive human cord blood progenitor cells and maintains the engraftment potential of scid repopulating cells
    T Rossmanith
    Department of Hematology and Oncology, III University Medical Center Frankfurt, Germany
    Stem Cells 19:313-20. 2001
    ..Flt-3 ligand (FL), stem cell factor (SCF), and thrombopoietin (TPO) are considered indispensable, the role of interleukin 3 (IL-3) is still controversial: it has been reported either to support or abrogate the reconstituting ability ..
  88. ncbi IL-3 affects endothelial cell-mediated smooth muscle cell recruitment by increasing TGF beta activity: potential role in tumor vessel stabilization
    Patrizia Dentelli
    Department of Internal Medicine, University of Torino, Corso Dogliotti 14, 10126 Torino, Italy
    Oncogene 23:1681-92. 2004
    ..Thus, the positive immunostaining for IL-3 by TILs in 'in situ' breast cancers sustains the possibility that early in tumor development, IL-3 can contribute to the chronic immaturity of these vessels...
  89. ncbi BCR-ABL and interleukin 3 promote haematopoietic cell proliferation and survival through modulation of cyclin D2 and p27Kip1 expression
    Y Parada
    Ludwig Institute for Cancer Research and Section of Virology and Cell Biology, Imperial College School of Medicine at St Mary's, Norfolk Place, London W2 1PG, United Kingdom
    J Biol Chem 276:23572-80. 2001
    ..Furthermore, our data also suggest that cyclin D2 functions upstream of p27(Kip1), cyclin E, and cyclin D3, and therefore, plays an essential part in integrating the signals from IL-3 and BCR-ABL with the pRB/E2F pathway...
  90. pmc The serine/threonine kinase Pim-2 is a transcriptionally regulated apoptotic inhibitor
    Casey J Fox
    Abramson Family Cancer Research Institute, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 17:1841-54. 2003
    ..These results establish Pim-2 as a direct link between growth factor-induced transcription and a novel, kinase-dependent pathway that promotes cell-autonomous survival...
  91. ncbi c-kit ligand mediates increased expression of cytosolic phospholipase A2, prostaglandin endoperoxide synthase-1, and hematopoietic prostaglandin D2 synthase and increased IgE-dependent prostaglandin D2 generation in immature mouse mast cells
    M Murakami
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    J Biol Chem 270:3239-46. 1995
    ....
  92. ncbi Protein kinase C plays an inhibitory role in interleukin 3- and interleukin 4-mediated mast cell proliferation
    E Chaikin
    Institute of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Biol Chem 265:22109-16. 1990
    b>Interleukin 3 (IL-3) is required for the survival and proliferation of mouse bone marrow derived mast cells (BMMC)...
  93. ncbi New selective amplifier genes containing c-Mpl for hematopoietic cell expansion
    Takeyuki Nagashima
    DNAVEC Research, Incorporated, 1 25 11, Kannondai, Tsukuba shi, Ibaraki ken 305 0856, Japan
    Biochem Biophys Res Commun 303:170-6. 2003
    ..The Mpl-type SAGs induced more potent proliferation of Ba/F3 and cynomolgus CD34(+) cells than the GCR-type SAG. One mutant Mpl-type SAG (Delta GCRMplTmR) successfully lost the responsiveness to TPO without affecting the Tm-dependence...
  94. ncbi Stem cell factor increases colony-forming unit-spleen number in vitro in synergy with interleukin-6, and in vivo in Sl/Sld mice as a single factor
    D M Bodine
    Clinical Hematology Branch NHLBI, Bethesda, MD 20892
    Blood 79:913-9. 1992
    ..These results show that SCF alone can increase CFU-S number in vivo, and in combination with other growth factors increases CFU-S numbers in vitro...
  95. pmc Hematopoietic progenitors express neural genes
    James Goolsby
    Department of Neurology, University of Maryland School of Medicine, and Multiple Sclerosis Center of Excellence, Veterans Affairs Medical Center, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 100:14926-31. 2003
    ..The fact that these CD34+ cells also express transcription factors (Rex-1 and Oct-4) that are found in early development elicits the hypothesis that they may be pluripotent embryonic-like stem cells...
  96. ncbi The lipocalin 24p3, which is an essential molecule in IL-3 withdrawal-induced apoptosis, is not involved in the G-CSF withdrawal-induced apoptosis
    Kenjirou Kamezaki
    The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Eur J Haematol 71:412-7. 2003
    ..Lipocalin 24p3, reported to be induced in hematopoietic cells by interleukin 3 (IL-3) depletion, induces hematopoietic cell apoptosis despite the presence of IL-3...
  97. ncbi PU.1 supports proliferation of immature erythroid progenitors
    Robert C Fisher
    Program in Stem Cell Biology and Regenerative Medicine, Shands Cancer Center, Department of Molecular Genetics and Microbiology, Box 100232, Room R4 293, University of Florida, Gainesville, FL 32610, USA
    Leuk Res 28:83-9. 2004
    ..1 target genes known to affect cytokine signaling and gene regulation in the erythroid lineage. These data suggest that PU.1 plays an important role in regulating the proliferation of immature erythroid progenitors...
  98. pmc Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells
    A Kawasaki
    Department of Hematology/Oncology, Osaka University Medical School, Osaka 565-0871, Japan
    J Cell Biol 152:275-87. 2001
    ..Also, the induced expression of AIM-1(K/R) in CMK cells provoked polyploidization up to 32N. These results suggested that downregulation of AIM-1 at M phase may be involved in abortive mitosis and polyploid formation of megakaryocytes...
  99. pmc CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells
    H Nakano
    Department of Medicine and Division of Cardiology, Duke University Medical Center, Durham, NC 27710, USA
    J Exp Med 194:1171-8. 2001
    ..Their survival is increased and they develop a DC-like morphology in interleukin 3 and CpG...
  100. ncbi Sodium caseinate induces expression and secretion of murine multipotent myeloid cell line 32D macrophage colony-stimulating factor
    Gerardo Ramos
    Laboratorio de Biología Celular y Molecular del Cáncer L 324, Facultad de Estudios Superiores FES Zaragoza, Universidad Nacional Autonoma de Mexico, Calle Batalla 5 de Mayo s n, Mexico City, 09230 Mexico
    Arch Med Res 35:109-13. 2004
    ....
  101. ncbi Chemokine SDF-1 enhances circulating CD34(+) cell proliferation in synergy with cytokines: possible role in progenitor survival
    J J Lataillade
    Laboratoire d Immunologie Cellulaire, centre de transfusion sanguine des armées Jean Julliard, Clamart Cedex, France
    Blood 95:756-68. 2000
    ..Furthermore, both adhesion-induced CXCR-4 overexpression and SDF-1 stimulating activity may be of clinical relevance for improving cell therapy settings in stem cell transplantation...

Research Grants62

  1. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..If successful, this grant could help explain the causes of iron deficiency anemia and could help in designing better drugs to combat iron deficiency anemia. ..
  2. The Role of PKR in a Novel IL-3 Signal Transduction Pathway
    William Stratford May; Fiscal Year: 2012
    b>Interleukin 3 (IL-3) is a multi-potential hematopoietic growth factor that activates cellular signaling pathways to mediate growth and differentiation of bone marrow progenitor cells...
  3. Computational Design of Small Molecule Drugs Inhibiting the Zinc Finger Transcrip
    David Sherris; Fiscal Year: 2010
    ..Work from this project will further advance our knowledge on zinc finger protein transcription factor/DNA interactions and may thus open the door to a whole new range of therapeutic agents. ..
  4. The Contribution of Chronic Inflammation to Pulmonary Adenocarcinoma
    MICHAEL LAWRENCE DOUGAN; Fiscal Year: 2010
    ....
  5. IL-4 Signal Transduction
    JOHN D COLGAN; Fiscal Year: 2013
    ..NFIL3 (nuclear factor, interleukin 3 regulated) is the most strongly induced transcription factor by IL-4 stimulation in a STAT6-dependent manner...
  6. Elucidation of Novel Anti-apoptotic Pathways in Erythropoiesis
    Yongping Wang; Fiscal Year: 2013
    ..The PI's clinical career in transfusion medicine will be nicely complemented by this research to understand and influence red blood cell formation. ..
  7. t(8;21) and SON in Blood Cell Proliferation and Differentiation
    Dong Er Zhang; Fiscal Year: 2013
    ..These studies will address important questions about hematopoiesis and leukemogenesis, which may provide valuable insight into the treatment of leukemia and other cancers. ..
  8. Role of Gab2 and Shp2 in Hematopoietic Signalling
    Benjamin G Neel; Fiscal Year: 2010
    ..We also will determine the mechanisms and consequences of Jak2 hyperactivation, and test whether Shp2 mutations may also play a role in adult MPD. ..
  9. Hematopoietic stem cell cytokine control of developmental vascularization
    George E Davis; Fiscal Year: 2013
    ..e. stem cell factor-SCF;interleukin 3- IL-3;and stromal-derived factor-1 alpha- SDF-1[unreadable]) control human endothelial cell (EC) tube ..
  10. IL3 GROWTH FACTOR SIGNALING
    William Stratford May; Fiscal Year: 2010
    ....
  11. MAST CELL MEMBRANE GLYCOLIPID EXPRESSION AND FUNCTION
    Michael Raizman; Fiscal Year: 1991
    ..The binding of cholera toxin to ganglioside GM1 on the plasma membranes of interleukin 3- dependent, mouse bone marrow culture-derived mast cells induces an increase in both the histamine content and ..
  12. PATHOGENESIS OF ALLERGIC DISEASES OF THE SKIN & LUNGS
    Allen Kaplan; Fiscal Year: 1992
    ..cell degranulation and determine its relationship to histamine-releasing cytokines we have identified such as interleukin 3 and GM-CSF...
  13. GP91-PHOX TRANSCRIPTION FACTORS AND HEMATOPOIESIS
    Elizabeth Eklund; Fiscal Year: 2000
    ..The third aim is to investigate the in vivo changes of proteins binding to the gp91phox promoter, an extension of the in vitro footprinting methods that have been utilized to date for this project. ..
  14. IN-VITRO AND IN-VIVO EFFECTS OF GM-CSF ON ADULT ANLL
    Camille Abboud; Fiscal Year: 1992
    ..responses to recombinant cytokines such as GM-CSF, granulocyte colony-stimulating factor (G-CSF), interleukin 3 (IL-3) and macrophage colony-stimulating factor (M-CSF); 3) perform binding studies of 125I-GM-CSF and G-CSF ..
  15. HUMAN IL3 ACTIVATION OF PROTEIN KINASE C AND BCL-2 GENE
    Yufang Shi; Fiscal Year: 2001
    ....
  16. HIV and Plasmacytoid dendritic cells in the thymus
    Christel Uittenbogaart; Fiscal Year: 2007
    Plasmacytoid dendritic cells (pDC) are lymphoid dendritic cells that express high levels of interleukin 3 (IL-3) receptor (CD 123) and produce type I interferons, cytokines with antiviral and immunoregulatory effects, pDC are present in ..
  17. DEVELOPMENT OF NEW SCID MOUSE MODELS FOR AIDS RESEARCH
    Leonard Shultz; Fiscal Year: 1999
    ..These small animal models will be tested for human lymphoid and hematopoietic stem cell engraftment and will be utilized for the evaluation of HIV vaccines. ..
  18. EOSINOPHIL APOPTOSIS IN ASTHMA
    Gerald Litwack; Fiscal Year: 2001
    ..Furthermore, manipulation of these pathways will be a key area for therapeutic intervention in asthma. ..
  19. IN VITRO STUDIES OF THE MECHANISM OF ANAPHYLAXIS
    FRANK AUSTEN; Fiscal Year: 1999
    ..cDNA library; the cDNA will be used to examine transcription of these proteins during phenotypic changes in interleukin 3 (IL-3)-dependent bone marrow- derived murine mast cells (BMMC) elicited by fibroblasts or during regulation of ..
  20. ROLE OF T LYMPHOCYTE FACTORS IN HEMOPOIESIS 0
    Michael Prystowsky; Fiscal Year: 1992
    ..These studies will further our understanding of the role that T lymphocytes play in regulating hemopoiesis...
  21. Targeting protein synthesis in AML
    ARTHUR FRANKEL; Fiscal Year: 2006
    ..These studies will hopefully lead to the design of pivotal phase II and III clinical trials which will define the role of DT388IL3 in the management of AML. ..
  22. EFFECT OF PERTUSSIS TOXIN ON HEMOPOIESIS
    Peter Quesenberry; Fiscal Year: 1991
    ..These studies should enable us to elucidate the first step of hemopoietic growth factor activation on bone marrow cells and indicate which metabolic pathways will be appropriate for further investigation...
  23. ERYTHROPOIETIN AND THE REGULATION OF ERYTHROPOIESIS
    Sanford Krantz; Fiscal Year: 2003
    ..These experiments have direct application to understanding the anemia of chronic disease and the increased apoptosis in MDS, which produces a marked decrease in human blood cells. ..
  24. POST-TRANSCRIPTIONAL CONTROL OF THE HEART AND LUNGS
    Carlos Gonzalez; Fiscal Year: 2004
    ..These studies will help us expand our knowledge on AREs and ARE-BPs, and how these interactions control the expression of mRNAs involved in cardiovascular and pulmonary diseases, as well as in cancers and immune disorders. ..
  25. HUMAN IMMUNODEFICIENCY VIRUS AND HEMATOPOIESIS
    Colin Sieff; Fiscal Year: 1993
    ..T cells are probably the only source of the multi-poietin interleukin 3 (IL-3), a CSF with profound effects on the survival and proliferation of hematopoietic progenitor cells: ..
  26. SRC-LIKE KINASES IN INTERLEUKIN-3 RECEPTOR SIGNALING
    Steven Anderson; Fiscal Year: 2001
    ..These approaches should also help to identify both substrates phosphorylated, as well as signaling pathways activated, in a Src kinase-dependent manner. ..
  27. STUDIES OF MEGAKARYOCYTOPOIESIS
    Samuel Burstein; Fiscal Year: 1991
    ....
  28. BASOPHIL ACTIVATION AND RECRUITMENT IN ALLERGIC DISEASE
    Bruce Bochner; Fiscal Year: 1993
    ....
  29. IMMUNOREGULATION OF ERYTHROPOIESIS
    Lee Levitt; Fiscal Year: 1992
    ..These studies should provide new information in the biology of hematopoietic immunoregulation and are relevant to our understanding of T cell membrane biology and T cell activation...
  30. CYTOKINES AND AIDS DEMENTIA COMPLEX
    Iain Campbell; Fiscal Year: 2000
    ....
  31. SIGNAL TRANSDUCTION BY TEL/TYROSINE KINASE FUSIONS
    David Sternberg; Fiscal Year: 2003
    ..The candidate's career goals and research career development plan are detailed in Section II of this proposal. ..
  32. IMMUNOPHYSIOLOGY OF KERATINOCYTE CYTOKINES
    Thomas Kupper; Fiscal Year: 1991
    ..produce factors now known to be identical to cytokines produced by immune cells (Interleukin 1 (IL-1), Interleukin 3 (IL-3), and granulocyte macrophage colony stimulating factor (GM-CSF))...
  33. CELL BIOLOGY OF MEGAKARYOCYTOPOIESIS
    Robert Leven; Fiscal Year: 1992
    ..In addition these studies may lead to a useful in vitro assay for the purification of thrombopoietin. Overall, a better understanding of the control of megakaryocytopoiesis will be the outcome of this project...
  34. HIV and Plasmacytoid dendritic cells in the thymus
    Christel Uittenbogaart; Fiscal Year: 2002
    ..pDC express high levels of interleukin 3 (IL-3) receptor (CD123) and are present in cord and peripheral blood, T cell areas of the lymph nodes and in ..
  35. REGULATION OF HEMATOPOIETIC GROWTH FACTOR INTERLEUKIN 4
    Melissa Brown; Fiscal Year: 1992
    ....
  36. IN UTERO TRANSPLANTATION IN BETA-THALASSEMIC
    Ewa Carrier; Fiscal Year: 2001
    ..The candidate will have developed an independent research program involving in utero transplantation and have progressed to a more advanced phase of her academic career. ..
  37. CHARACTERIZATION OF BONE MARROW DERIVED GRANULOCYTES
    James Bender; Fiscal Year: 1991
    ..The fifth goal is to determine the relationship between proliferative capacity and development of functional activity in maturing bone marrow granulocytes...
  38. IL3,IL5, and GM-CSF Signaling and Asthma
    Christian Schindler; Fiscal Year: 2004
    ..Specifically we propose to: 1. Further characterize StIP1. 2. Functionally characterize the STAT four-bundle-helix. 3. Explore the role of Stat5 signal decay in primary leukocytes. ..
  39. PROLIFERATIVE SIGNALS FROM IL-3 RECEPTORS TO NUCLEUS
    Theodore Gabig; Fiscal Year: 1993
    ..The long term goals of this study are to purify the most relevant or novel proteins involved in this signalling process, to clone and sequence their corresponding cDNA's, and define their function in the signalling process...
  40. MOLECULAR BASIS OF HEMATOPOIETIC GROWTH FACTOR SIGNALING
    Stephen Peiper; Fiscal Year: 1993
    ....
  41. Mechanism(s) of macrophage-mediated demyelination
    AFIF HENTATI; Fiscal Year: 2006
    ....
  42. SERUM FREE MEDIUM KIT FOR THE CULTURE OF HUMAN CELLS
    Ronald Brown; Fiscal Year: 1992
    ..During the course of these studies they will use standard clonal and liquid culture techniques, cell identification techniques and assess a variety of purified culture reagents...
  43. CSF DEFICIENCY AND ANTITUMOR IMMUNITY
    Glenn Dranoff; Fiscal Year: 2004
    ..3). To develop strategies for reconstituting tumor defense in mice deficient in GM-CSF, IL-3, and IFN-gamma. ..
  44. MOLECULAR GENETICS OF HUMAN RED BLOOD CELL SPECTRIN
    JOHN WINKELMANN; Fiscal Year: 1992
    ....
  45. INDEPENDENT AND COORDINATE REGULATION OF IL3 AND GMCSF
    Douglas Taylor; Fiscal Year: 1999
    ....
  46. STRUCTURE/FUNCTION ANALYSIS OF IL3 AND ITS RECEPTOR
    Kenneth Kaushansky; Fiscal Year: 2000
    ..Kaushansky and colleagues propose to use a novel approach to identify ligand binding sites on the interleukin 3 (IL-3) receptor (IL-3R)...
  47. Role of Lipocalin 24p3 in Apoptosis and Leukemia
    Michael R Green; Fiscal Year: 2010
    ..undergoing maximum transcriptional stimulation following induction of apoptosis by cytokine-deprivation of interleukin 3 (IL-3) dependent cells...
  48. RETINOID RESPONSIVE GENES/EARLY MYELOID DIFFERENTIATION
    Noel Maun; Fiscal Year: 2001
    ..abstract_text> ..
  49. Cytokine Regulated Growth Of Hematopoietic Cells
    James Ihle; Fiscal Year: 2007
    ..The focus of the studies have been with interleukin 3 (IL-3), a cytokine that affects cells of many stages of hematopoiesis but also has utilized receptors such as ..
  50. CRYSTALLIZATION OF IL-3 AN ANTIBODY MEDIATED APPROACH
    THOMAS HOPP; Fiscal Year: 1991
    DESCRIPTION: (Adapted from the applicant's abstract) Interleukin 3 will be crystallized using a new approach that may provide a general method for generating crystals of recombinant proteins. Human IL-3 will be produced in E...
  51. DOPAMINE TRANSPORTER: SUBSTRATE & COCAINE BINDING SITES
    JONATHAN JAVITCH; Fiscal Year: 2007
    ..4) To map the intracellular and extracellular accessibility during different functional states of substituted-cysteines through IL1 and IL3 of DAT. ..
  52. AIDS RELATED LYMPHOMAS--CLINICAL AND BIOLOGIC STUDIES
    Leo Gordon; Fiscal Year: 1993
    ..The aims of the proposal will be 1) To determine the biologically active dose of the growth factor interleukin 3 (IL-3) in a Phase I trial in neutropenic HIV positive patients, and to asses its effect on p24 antigen ..
  53. REGULATION OF MYELOID PROGENITOR CELL DIFFERENTIATION
    LOUIS PELUS; Fiscal Year: 1992
    ..The interegulatory roles of recombinant murine and human growth factors G-CSF, GM-CSF, M-CSF (CSF1) Interleukin 3 and Interkeukin 1, and inhibitors, interferons alpha, beta and gamma Tumor Necrosis Factor (TNF) and ..
  54. EXPRESSION AND REPRESSION OF THE IL-3 GENE
    Maurice Wolin; Fiscal Year: 1993
    ..In this manner we will characterize the expression of IL-3 in cells in response to conventional T-cell activators and to increases in the intracellular second messenger, cyclic AMP...
  55. MECHANISMS OF CHRONIC PROSTATITIS
    Don Kreutzer; Fiscal Year: 2000
    ....
  56. PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOCYTES
    Carleton Stewart; Fiscal Year: 1991
    ..Results of these studies will have an enormous impact on our understanding of how a normal cell's production and function is controlled in health and disease...
  57. CYTOKINE SIGNALING PATHWAYS REGULATING A G1 CHECKPOINT
    FREDERICK QUELLE; Fiscal Year: 2003
    ..Finally, we will directly assay the status of G/1 cell cycle regulators, including cyclin D-dependent kinases and their inhibitors, and correlate their activities with the UV-induction and cytokine release of the G/1 checkpoint. ..
  58. ERBB-4 TARGETED THERAPY WITH RECOMBINANT HEREGULIN
    Dajun Yang; Fiscal Year: 2001
    ..This program is designed to ultimately generate specific, effective recombinant toxins with unique targets that have therapeutic potential by themselves or as adjuvant to conventional chemotherapy into clinical trial in near future. ..
  59. MECHANISMS OF HEMOPOIETIC IMMORTALIZATION
    H Boswell; Fiscal Year: 1991
    ..The mode of expression of the newly transfected genes will be studied to further define the potential role of autocrine regulatory mechanisms in leukemogenesis...
  60. IL-3 RESPONSE--CONTROL BY G PROTEINS
    Theodore Gabig; Fiscal Year: 1991
    ..This assay will be used in the subsequent purification of reconstitutively active G protein-coupled PIP2- specific phospholipase C from the plasma membranes of IL-3 dependent cell lines...
  61. CHARACTERIZATION OF TWO NOVEL BCL-2-ASSCOCIATED PROTEINS
    Daniel Johnson; Fiscal Year: 1999
    ..Investigations of interactions between the BAP proteins and other members of the Bcl-2 protein family will be performed in vitro, using purified recombinant proteins. ..
  62. EFFECT OF RETROVIRAL INFECTION ON BONE MARROW
    Michael Prystowsky; Fiscal Year: 1992
    ..These studies should give us insignt into the effect of retroviral infection on hemopoiesis and should give us experience on how to use exogenous growth factors to reserve some of these effects...