factor x

Summary

Summary: Storage-stable glycoprotein blood coagulation factor that can be activated to factor Xa by both the intrinsic and extrinsic pathways. A deficiency of factor X, sometimes called Stuart-Prower factor deficiency, may lead to a systemic coagulation disorder.

Top Publications

  1. ncbi Coagulation factor X shields adenovirus type 5 from attack by natural antibodies and complement
    Zhili Xu
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Nat Med 19:452-7. 2013
  2. pmc Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors
    Raul Alba
    British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK
    Blood 116:2656-64. 2010
  3. ncbi Coagulation factor X activates innate immunity to human species C adenovirus
    Konstantin Doronin
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Science 338:795-8. 2012
  4. pmc Coagulation factor X mediates adenovirus type 5 liver gene transfer in non-human primates (Microcebus murinus)
    R Alba
    Division of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK
    Gene Ther 19:109-13. 2012
  5. pmc An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo
    B Gotoh
    Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan
    EMBO J 9:4189-95. 1990
  6. pmc The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors
    Jennifer Disse
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 286:5756-67. 2011
  7. pmc Substitution of adenovirus serotype 3 hexon onto a serotype 5 oncolytic adenovirus reduces factor X binding, decreases liver tropism, and improves antitumor efficacy
    Joshua J Short
    Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Mol Cancer Ther 9:2536-44. 2010
  8. ncbi Six novel mutations including triple heterozygosity for Phe31Ser, 514delT and 516T-->G factor X gene mutations are responsible for congenital factor X deficiency in patients of Nepali and Indian origin
    G Jayandharan
    Department of Hematology, Christian Medical College, Vellore, India
    J Thromb Haemost 3:1482-7. 2005
  9. ncbi Co-localization of Protein Z, Protein Z-Dependent protease inhibitor and coagulation factor X in human colon cancer tissue: implications for coagulation regulation on tumor cells
    Ewa Sierko
    Department of Oncology, Medical University, Bialystok, Poland
    Thromb Res 129:e112-8. 2012
  10. ncbi A hexon-specific PEGylated adenovirus vector utilizing blood coagulation factor X
    Hayato Matsui
    Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    Biomaterials 33:3743-55. 2012

Research Grants

Detail Information

Publications263 found, 100 shown here

  1. ncbi Coagulation factor X shields adenovirus type 5 from attack by natural antibodies and complement
    Zhili Xu
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Nat Med 19:452-7. 2013
    Adenovirus type 5 (Ad5) specifically binds coagulation factor X (FX), and FX is normally essential for intravenously injected Ad5 vectors to transduce the liver...
  2. pmc Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors
    Raul Alba
    British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK
    Blood 116:2656-64. 2010
    ..Recently, we demonstrated that coagulation factor X (FX) binds to Ad5-hexon protein at high affinity to mediate hepatocyte transduction after intravascular delivery...
  3. ncbi Coagulation factor X activates innate immunity to human species C adenovirus
    Konstantin Doronin
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Science 338:795-8. 2012
    ..We modeled the interface of human species C adenovirus (HAdv) interaction with coagulation factor X (FX) and introduced a mutation that abrogated formation of the HAdv-FX complex...
  4. pmc Coagulation factor X mediates adenovirus type 5 liver gene transfer in non-human primates (Microcebus murinus)
    R Alba
    Division of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK
    Gene Ther 19:109-13. 2012
    Coagulation factor X (FX)-binding ablated adenovirus type 5 (Ad5) vectors have been genetically engineered to ablate the interaction with FX, resulting in substantially reduced hepatocyte transduction following intravenous administration ..
  5. pmc An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo
    B Gotoh
    Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan
    EMBO J 9:4189-95. 1990
    ..required for Ca2+ binding, and was found to be highly homologous, if not identical, to the blood clotting factor X(FX), a member of the prothrombin family...
  6. pmc The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors
    Jennifer Disse
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 286:5756-67. 2011
    ....
  7. pmc Substitution of adenovirus serotype 3 hexon onto a serotype 5 oncolytic adenovirus reduces factor X binding, decreases liver tropism, and improves antitumor efficacy
    Joshua J Short
    Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Mol Cancer Ther 9:2536-44. 2010
    ..In this regard, it has recently been shown that the Ad5 capsid binds to the blood coagulation factor X (FX) via the Ad5 hexon protein. This interaction mediates the majority of Ad5 liver transduction...
  8. ncbi Six novel mutations including triple heterozygosity for Phe31Ser, 514delT and 516T-->G factor X gene mutations are responsible for congenital factor X deficiency in patients of Nepali and Indian origin
    G Jayandharan
    Department of Hematology, Christian Medical College, Vellore, India
    J Thromb Haemost 3:1482-7. 2005
    b>Factor X (FX) deficiency is a rare (1 : 100000) autosomal recessive disorder caused by heterogeneous mutations in FX gene. We have studied the molecular basis this disease in six Indian and one Nepali patients...
  9. ncbi Co-localization of Protein Z, Protein Z-Dependent protease inhibitor and coagulation factor X in human colon cancer tissue: implications for coagulation regulation on tumor cells
    Ewa Sierko
    Department of Oncology, Medical University, Bialystok, Poland
    Thromb Res 129:e112-8. 2012
    Several hemostatic system components, including factor X (FX), contribute to cancer progression. The Protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex directly inhibits factor Xa proteolytic activity...
  10. ncbi A hexon-specific PEGylated adenovirus vector utilizing blood coagulation factor X
    Hayato Matsui
    Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    Biomaterials 33:3743-55. 2012
    ..we developed a new method for hexon-specific PEGylation by mixing Ad vectors with PEGylated blood coagulation factor X (FX) (PEG-FX)...
  11. ncbi Phospholipid regulates the activation of factor X by tissue factor/factor VIIa (TF/VIIa) via substrate and product interactions
    James J Hathcock
    Department of Medicine, Mount Sinai School of Medicine, Box 1269, One Gustave Levy Place, New York, New York 10029, USA
    Biochemistry 44:8187-97. 2005
    ..to already relipidated TF (30/70 PS/PC) and found that added lipid can both enhance and inhibit the rate of factor X (F.X) activation. Using active-site-inhibited F.Xa we demonstrate that F...
  12. pmc Cell entry and trafficking of human adenovirus bound to blood factor X is determined by the fiber serotype and not hexon:heparan sulfate interaction
    Stephanie Corjon
    University Lyon 1, Inra UMR 754, Retrovirus and Comparative Pathology, Lyon, France
    PLoS ONE 6:e18205. 2011
    ..administered intravenously accumulate in the liver as the result of their direct binding to blood coagulation factor X (FX) and subsequent interaction of the FX-HAdV5 complex with heparan sulfate proteoglycan (HSPG) at the surface ..
  13. ncbi Macrophages synthesize factor X and secrete factor X/Xa-containing prothrombinase activity into the surrounding medium
    G Pejler
    Swedish University of Agricultural Sciences, Department of Veterinary Medical Chemistry, The Biomedical Center, Uppsala
    Thromb Haemost 84:429-35. 2000
    ..synovial cells from rheumatoid arthritis lesions express prothrombinase activity that was inhibited by anti-Factor X antibodies...
  14. ncbi Singlet oxygen inactivates fibrinogen, factor V, factor VIII, factor X, and platelet aggregation of human blood
    T W Stief
    Institute of Clinical Chemistry, Philipps University, D 35033, Marburg, Germany
    Thromb Res 97:473-80. 2000
    ..Plasmatic coagulation factors sensible to oxidation are fibrinogen, factor V, factor VIII, and factor X with a 50% effective dose of 2-3 mmol/l NaOCl or taurine-chloramine. Chloramines or chloramine-like agents (e.g...
  15. ncbi Adenovirus serotype 5 hexon mediates liver gene transfer
    Simon N Waddington
    Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free and University College Medical School, London NW3 2PF, UK
    Cell 132:397-409. 2008
    ..Liver infection by the FX-Ad5 complex is mediated through a heparin-binding exosite in the FX serine protease domain. This study reveals an unanticipated function for hexon in mediating liver gene transfer in vivo...
  16. ncbi Two coagulation factor X activators from Vipera a. ammodytes venom with potential to treat patients with dysfunctional factors IXa or VIIa
    Adrijana Leonardi
    Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova Street 39, SI 1000 Ljubljana, Slovenia
    Toxicon 52:628-37. 2008
    Two activators of coagulation factor X, 58kDa VAFXA-I and 70kDa VAFXA-II, were purified from the venom of long-nosed viper (Vipera ammodytes ammodytes) by chromatography on gel filtration, affinity, ion-exchange and hydroxyapatite media...
  17. ncbi Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes. A surface plasmon resonance study
    Eva Maria Erb
    Department of Clinical Chemistry, University Hospital Malmo, Lund University, Malmo, Sweden
    Eur J Biochem 269:3041-6. 2002
    The interaction of blood coagulation factor X and its Gla-containing fragments with negatively charged phospholipid membranes composed of 25 mol% phosphatidylserine (PtdSer) and 75 mol% phosphatidylcholine (PtdCho) was studied by surface ..
  18. ncbi Kinetics of coagulation factor X activation by platelet-bound factor IXa
    R Rawala-Sheikh
    Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140
    Biochemistry 29:2606-11. 1990
    ..5 nM), saturable binding sites for factor IXa that are involved in factor X activation [Ahmad, S.S., Rawala-Sheikh, R., & Walsh, P.N. (1989) J. Biol. Chem. 264, 3244-3251]...
  19. ncbi A factor X-TFPI hybrid protein inhibits B16 melanoma metastatic growth in mice
    Thomas J Girard
    Thromb Res 121:427-8. 2007
  20. ncbi Structure of two genes encoding parallel prothrombin activators in Tropidechis carinatus snake: gene duplication and recruitment of factor X gene to the venom gland
    M A Reza
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    J Thromb Haemost 5:117-26. 2007
    ..For example, in Tropidechis carinatus, trocarin D exists in the venom and acts as toxin, whereas coagulation factor (F) X exists in plasma and plays a role in hemostasis...
  21. ncbi Two parallel prothrombin activator systems in Australian rough-scaled snake, Tropidechis carinatus. Structural comparison of venom prothrombin activator with blood coagulation factor X
    Abu Reza
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Thromb Haemost 93:40-7. 2005
    ..Here, we report the complete cDNA sequence encoding the blood coagulation factor X (FX) from the liver of T. carinatus. Deduced T...
  22. ncbi Vitamin K epoxide reductase significantly improves carboxylation in a cell line overexpressing factor X
    Yan Mei Sun
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Blood 106:3811-5. 2005
    Previously we reported that we could increase the fraction of carboxylated factor X by reducing the affinity of the propeptide for its binding site on human gamma glutamyl carboxylase. We attributed this to an increased turnover rate...
  23. ncbi New insights into the functions and N-glycan structures of factor X activator from Russell's viper venom
    Hong Sen Chen
    Graduate Institute of Biochemical Sciences, National Taiwan University, Taiwan
    FEBS J 275:3944-58. 2008
    The coagulation factor X activator from Russell's viper venom (RVV-X) is a heterotrimeric glycoprotein. In this study, its three subunits were cloned and sequenced from the venom gland cDNAs of Daboia siamensis...
  24. ncbi Factor X deficiency: clinical manifestation of 102 subjects from Europe and Latin America with mutations in the factor 10 gene
    F H Herrmann
    Institute of Human Genetics, University of Greifswald, Greifswald, and Hess Children s Hospital, Klinikum Bremen Mitte, Bremen, Germany
    Haemophilia 12:479-89. 2006
    Inherited factor X deficiency (FXD) is a rare (1:1,000,000) recessive bleeding disorder...
  25. ncbi Activation peptides prolong the murine plasma half-life of human factor VII
    Linda Johansson
    Haemostasis Biochemistry, Novo Nordisk A S, Måløv, Denmark
    Blood 117:3445-52. 2011
    ..Our data support that activation peptides function as plasma retention signals and constitute a new manner to extend the half-life of FVII(a)...
  26. pmc Differential effects of murine and human factor X on adenovirus transduction via cell-surface heparan sulfate
    Anne K Zaiss
    Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 286:24535-43. 2011
    Serum coagulation factor X (FX) is proposed to play a major role in adenovirus tropism, promoting transduction by bridging the virus to cell-surface heparan sulfate proteoglycans (HSPGs)...
  27. ncbi Factor X activator from Vipera lebetina venom is synthesized from different genes
    Ene Siigur
    National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia
    Biochim Biophys Acta 1702:41-51. 2004
    Vipera lebetina venom contains specific coagulant Factor X activator (VLFXA) that cleaves the Arg52-Ile53 bond in the heavy chain of human factor X...
  28. pmc Herpes simplex virus type 1-encoded glycoprotein C contributes to direct coagulation factor X-virus binding
    Joel R Livingston
    Canadian Blood Services, Research and Development Department, University of British Columbia Centre for Blood Research, Department of Pathology and Laboratory Medicine, 2350 Health Sciences Mall, Vancouver, BC, Canada, V6T 1Z3
    Biochem J 393:529-35. 2006
    ..recently to initiate blood coagulation by FVIIa (activated Factor VII)-dependent proteolytic activation of FX (Factor X)...
  29. pmc Inhibition of acute vascular thrombosis in chimpanzees by an anti-human tissue factor antibody targeting the factor X binding site
    Jin an Jiao
    Sunol Molecular Corporation, Miramar, Florida, USA
    Thromb Haemost 103:224-33. 2010
    ..evaluate whether a human/mouse chimeric monoclonal antibody (ALT-836, formerly known as Sunol-cH36) blocking the factor X/factor IX (FX/FIX) binding site of tissue factor could achieve similar clinical benefits in an arterial ..
  30. ncbi Thrombin-activable factor X re-establishes an intrinsic amplification in tenase-deficient plasmas
    Virginie B Louvain-Quintard
    INSERM U428, Faculte de Pharmacie, Universite Paris V, 75270 Paris, France
    J Biol Chem 280:41352-9. 2005
    Classical hemophilia results from a defect of the intrinsic tenase complex, the main factor X (FX) activator. Binding of factor VIIa to tissue factor triggers coagulation, but little amplification of thrombin production occurs...
  31. ncbi The N-terminal epidermal growth factor-like domain in factor IX and factor X represents an important recognition motif for binding to tissue factor
    Degang Zhong
    Department of Internal Medicine and Department of Pharmacological and Physiological Sciences, Saint Louis University Health Sciences Center, St Louis, Missouri 63110, USA
    J Biol Chem 277:3622-31. 2002
    ..During physiologic clotting, the factor VIIa-tissue factor (FVIIa*TF) complex activates both factor IX (FIX) and factor X (FX). FVIIa represents the enzyme, and TF represents the membrane-bound cofactor for this reaction...
  32. ncbi Two subpopulations of thrombin-activated platelets differ in their binding of the components of the intrinsic factor X-activating complex
    M A Panteleev
    National Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia
    J Thromb Haemost 3:2545-53. 2005
    ....
  33. ncbi Of four mutations in the factor VII gene in Tunisian patients, one novel mutation (Ser339Phe) in three unrelated families abrogates factor X activation
    Yonit Fromovich-Amit
    Amalia Biron Research Institute of Thrombosis and Hemostasis, Chaim Sheba Medical Center, Tel Hashomer, Israel
    Blood Coagul Fibrinolysis 16:369-74. 2005
    ..of commercial recombinant activated FVII or recombinant wild-type FVII and was normally activated by activated factor X. The major defect of FVII Ser339Phe was its inability to activate factor X in the presence of tissue factor...
  34. ncbi Interaction of the A1 subunit of factor VIIIa and the serine protease domain of factor X identified by zero-length cross-linking
    K A Lapan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry Rochester, New York, USA
    Thromb Haemost 80:418-22. 1998
    We have previously used a solid phase binding assay to localize a Factor X (FX) interactive site to the acidic C-terminus of the A1 subunit of FVIIIa (Lapan KA, Fay PJ. J Biol Chem 1997; 272: 2082-2088)...
  35. pmc Crystal structure of protein Z-dependent inhibitor complex shows how protein Z functions as a cofactor in the membrane inhibition of factor X
    Zhenquan Wei
    Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom
    Blood 114:3662-7. 2009
    ..PZ) binds to PZ-dependent inhibitor (ZPI) and accelerates the inhibition of the coagulation protease, activated factor X (FXa), in the presence of phospholipids and Ca2+. A 2...
  36. ncbi A novel inhibitor of factor X activation from the salivary glands of the bed bug Cimex lectularius
    J G Valenzuela
    Department of Entomology, University of Arizona, Tucson 85721, USA
    Exp Parasitol 83:184-90. 1996
    ..The anticoagulant principle inhibited the activation of factor X to factor Xa in the tenase complex (FVIII, FIXa, FX, phospholipids, and calcium)...
  37. ncbi Factor X levels, polymorphisms in the promoter region of factor X, and the risk of venous thrombosis
    M C de Visser
    Hemostasis and Thrombosis Research Center, Dept of Hematology, Leiden University Medical Center, The Netherlands
    Thromb Haemost 85:1011-7. 2001
    ..study on venous thrombosis (Leiden Thrombophilia Study, LETS) we investigated whether elevated coagulation factor X (FX) levels are a risk factor for venous thrombosis and whether FX levels are determined by polymorphisms in the ..
  38. ncbi Activation and inactivation of human factor X by proteases derived from Ficus carica
    Günter Richter
    Baxter BioScience, Vienna, Austria
    Br J Haematol 119:1042-51. 2002
    ..the prothrombin time of normal plasmas and plasmas deficient in coagulation factors, except plasma deficient in factor X (FX) and generated activated FX (FXa) in defibrinated plasma...
  39. pmc Requirements for receptor engagement during infection by adenovirus complexed with blood coagulation factor X
    Angela C Bradshaw
    Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS Pathog 6:e1001142. 2010
    Human adenoviruses from multiple species bind to coagulation factor X (FX), yet the importance of this interaction in adenovirus dissemination is unknown...
  40. ncbi Adenovirus 5 fibers mutated at the putative HSPG-binding site show restricted retargeting with targeting peptides in the HI loop
    Angelika B Kritz
    BHF Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK
    Mol Ther 15:741-9. 2007
    ..Cy3 labeling studies showed retarded trafficking of S*-containing fibers. Taken together, our data demonstrates that KO1S* mutant fibers are ineffective for cell retargeting strategies...
  41. pmc Identification of coagulation factor (F)X binding sites on the adenovirus serotype 5 hexon: effect of mutagenesis on FX interactions and gene transfer
    Raul Alba
    British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, G12 8TA, UK
    Blood 114:965-71. 2009
    Recent studies have demonstrated the importance of coagulation factor X (FX) in adenovirus (Ad) serotype 5-mediated liver transduction in vivo. FX binds to the adenovirus hexon hypervariable regions (HVRs)...
  42. pmc Effect of neutralizing sera on factor x-mediated adenovirus serotype 5 gene transfer
    Alan L Parker
    British Heart Foundation Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, 126 University Place, University of Glasgow, Glasgow G12 8TA, United Kingdom
    J Virol 83:479-83. 2009
    ..When such vectors are delivered intravenously, hepatocyte transduction is mediated by the hexon-coagulation factor X (FX) interaction...
  43. pmc A cluster of basic amino acids in the factor X serine protease mediates surface attachment of adenovirus/FX complexes
    Margaret R Duffy
    British Heart Foundation Glasgow Cardiovascular Research Centre, College of Medical, Veterinary and Life Sciences, University of Glasgow, 126 University Place, Glasgow G128TA, United Kingdom
    J Virol 85:10914-9. 2011
    ..following intravenous administration of adenovirus 5 (Ad5) is mediated by interaction between coagulation factor X (FX) and the hexon...
  44. pmc Coagulation factors IX and X enhance binding and infection of adenovirus types 5 and 31 in human epithelial cells
    Mari I Jonsson
    Division of Virology, Department of Clinical Microbiology, Umea University, SE 901 85 Umea, Sweden
    J Virol 83:3816-25. 2009
    ....
  45. ncbi Factor X deficiency
    Marzia Menegatti
    Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Department of Medicine and Medical Specialties, IRCCS Maggiore Hospital, Mangiagalli and Regina Elena Foundation, University of Milan, 20122 Milan, Italy
    Semin Thromb Hemost 35:407-15. 2009
    b>Factor X (FX) deficiency is a rare, recessively inherited bleeding disorder representing 10% of all rare bleeding diseases and affecting 1 in every 1,000,000 people...
  46. ncbi Factor X deficiency
    James Uprichard
    Haemophilia Centre and Haemostasis Unit, Royal Free Hospital, London, UK
    Blood Rev 16:97-110. 2002
    b>Factor X is one of the vitamin K-dependent serine proteases. It plays a crucial role in the coagulation cascade, as the first enzyme in the common pathway of thrombus formation...
  47. ncbi The local phospholipid environment modulates the activation of blood clotting
    Andrew W Shaw
    Department of Chemistry, College of Medicine, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Biol Chem 282:6556-63. 2007
    ..tissue factor complex. We also addressed whether this enzyme requires a pool of membrane-bound protein substrate (factor X) for efficient catalysis, or alternatively if it could efficiently activate factor X, which binds directly to the ..
  48. pmc Ixolaris binding to factor X reveals a precursor state of factor Xa heparin-binding exosite
    Robson Q Monteiro
    Instituto de Bioquimica Medica, Centro de Ciencias de Saude, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    Protein Sci 17:146-53. 2008
    ..Ixolaris may be a useful tool to study the physiological role of the FX HBPE and to evaluate this domain as a target for anticoagulant drugs...
  49. ncbi CMV-beta-actin promoter directs higher expression from an adeno-associated viral vector in the liver than the cytomegalovirus or elongation factor 1 alpha promoter and results in therapeutic levels of human factor X in mice
    L Xu
    Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA
    Hum Gene Ther 12:563-73. 2001
    ..An AAV vector with the CMV-beta-actin promoter upstream of the coagulation protein human factor X (hFX) was injected intravenously into neonatal mice. This resulted in expression of hFX at 548 ng/ml (6...
  50. ncbi Effects of pre-analytical variables on the anti-activated factor X chromogenic assay when monitoring unfractionated heparin and low molecular weight heparin anticoagulation
    David L McGlasson
    59th Clinical Research Squadron, Wilford Hall Medical Center, 2200 Berquist Drive, Lackland AFB, TX 78236 5300, USA af mil
    Blood Coagul Fibrinolysis 16:173-6. 2005
    The purpose of this study was to determine whether the anti-activated factor X (anti-FXa) assay is less affected by pre-analytical variables in monitoring patients on unfractionated heparin (UFH) and low molecular weight heparin (LMWH) ..
  51. ncbi Successful treatment of systemic amyloidosis with hepatic involvement and factor X deficiency by high dose melphalan chemotherapy and autologous stem cell reinfusion
    Dimitri A Breems
    Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands
    Eur J Haematol 72:181-5. 2004
    ..We present two patients with a severe factor X deficiency and hepatomegaly as the presenting abnormalities of systemic amyloidosis...
  52. ncbi Effect of factor X inhibition on coagulation activation and cytokine induction in human systemic inflammation
    Ursula M Hollenstein
    Department of Clinical Pharmacology, Division of Infectious Diseases, Hanusch Krankenhaus, University of Tromso, Tromso, Norway
    J Infect Dis 186:1270-6. 2002
    ..Danaparoid therefore selectively attenuates endotoxin-induced coagulopathy, even with delayed administration when coagulation activation is well under way...
  53. pmc Crystal structure of an anticoagulant protein in complex with the Gla domain of factor X
    H Mizuno
    Department of Biotechnology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305 8602, Japan
    Proc Natl Acad Sci U S A 98:7230-4. 2001
    ..b>Factor X-binding protein (X-bp), an anticoagulant protein from snake venom, specifically binds to the Gla domain of factor ..
  54. ncbi Homozygous Factor X gene mutations Gly380Arg and Tyr163delAT are associated with perinatal intracranial hemorrhage
    Falko H Herrmann
    Greifswald University Hospital, Greifswald, Germany
    J Pediatr 146:128-30. 2005
    Intracranial hemorrhage (ICH) is a severe complication of Factor X (FX) deficiency. We report 6 homozygous patients with central nervous system (ICH) bleeds...
  55. ncbi Blood coagulation, fibrinolytic activity and lipid profile in subclinical thyroid disease: subclinical hyperthyroidism increases plasma factor X activity
    Cihangir Erem
    Karadeniz Technical University Faculty of Medicine, Department of Internal Medicine, Division of Endocrinology and Metabolism, Trabzon, Turkey
    Clin Endocrinol (Oxf) 64:323-9. 2006
    ....
  56. ncbi The interface between the EGF2 domain and the protease domain in blood coagulation factor IX contributes to factor VIII binding and factor X activation
    Caroline Fribourg
    Department of Plasma Proteins, Sanquin Research, 1066 CX Amsterdam, The Netherlands
    Biochemistry 45:10777-85. 2006
    ..All FIXa mutants, however, exhibited a reduced level of factor X (FX) activation...
  57. pmc Targeting adenoviruses with factor x-single-chain antibody fusion proteins
    Christopher Y Chen
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA
    Hum Gene Ther 21:739-49. 2010
    Abstract It has been shown that blood clotting factors, including factor X (FX), bind to the adenovirus serotype 5 (Ad5) hexon protein and target the virus to liver hepatocytes after intravenous injection...
  58. pmc Adenovirus serotype 5 hexon is critical for virus infection of hepatocytes in vivo
    O Kalyuzhniy
    Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195 7720, USA
    Proc Natl Acad Sci U S A 105:5483-8. 2008
    ..Here, we report that the major Ad5 capsid protein, hexon, binds human coagulation factor X (FX) with an affinity of 229 pM...
  59. pmc Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors
    Jenny A Greig
    British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow G12 8TA, UK
    Mol Ther 17:1683-91. 2009
    The binding of coagulation factor X (FX) to the hexon of adenovirus (Ad) 5 is pivotal for hepatocyte transduction...
  60. ncbi Ixolaris, a novel recombinant tissue factor pathway inhibitor (TFPI) from the salivary gland of the tick, Ixodes scapularis: identification of factor X and factor Xa as scaffolds for the inhibition of factor VIIa/tissue factor complex
    Ivo M B Francischetti
    Section of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0425, USA
    Blood 99:3602-12. 2002
    ..Ixolaris was expressed in insect cells and shown to inhibit factor VIIa (FVIIa)/tissue factor (TF)-induced factor X (FX) activation with an inhibitory concentration of 50% (IC(50)) in the picomolar range...
  61. pmc Antibodies against the activated coagulation factor X (FXa) in the antiphospholipid syndrome that interfere with the FXa inactivation by antithrombin
    Yao Hsu Yang
    Division of Rheumatology, Department of Medicine, University of California Los Angeles, 1000 Veteran Avenue, Los Angeles, CA 90095, USA
    J Immunol 177:8219-25. 2006
    ..Considering that activated coagulation factor X (FXa) is homologous to thrombin in the catalytic domains and is also regulated primarily by AT, we hypothesized ..
  62. ncbi Mechanisms of interactions of factor X and factor Xa with the acidic region in the factor VIII A1 domain
    Keiji Nogami
    Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 279:33104-13. 2004
    The 337-372 sequence of the factor VIIIa A1 subunit contains interactive sites for both zymogen factor X and the active enzyme, factor Xa...
  63. ncbi Plasmin converts factor X from coagulation zymogen to fibrinolysis cofactor
    E L Pryzdial
    Research and Development Department, Canadian Blood Services and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario K1G 4J5, Canada
    J Biol Chem 274:8500-5. 1999
    ..chromogenic assays, the fibrinolysis protease plasmin was found to irreversibly inhibit the pivotal function of factor X (FX) in coagulation...
  64. ncbi Binding of plasminogen and tissue plasminogen activator to plasmin-modulated factor X and factor Xa
    J E Grundy
    R and D Department, Canadian Blood Services, 1800 Alta Vista Drive, Ottawa, Ontario, Canada K1G 4J5
    Biochemistry 40:6293-302. 2001
    ..t-PA acceleration by FXgamma(47/13) or FXagamma(33/13) may therefore involve simultaneous presentation of two substrate molecules...
  65. ncbi Incorporation of blood clotting factor X into phospholipid model membranes: fluorescence microscopy imaging and subphase effects surrounding the lipid phase transition region
    Jeffrey Craft
    Department of Chemistry, Saint Louis University, 3501 Laclede Avenue, St Louis, MO 63103 2010, USA
    J Colloid Interface Sci 268:181-7. 2003
    b>Factor X is a blood clotting protein that associates at membrane surfaces to become activated during the coagulation cascade...
  66. pmc Activation of bovine factor X (Stuart factor): conversion of factor Xaalpha to factor Xabeta
    K Fujikawa
    Proc Natl Acad Sci U S A 72:3359-63. 1975
    Bovine factor X (molecular weight 55,100) is a blood coagulation factor present in plasma in a precursor or zymogen form. It is a glycoprotein which has been isolated as a two-chain structure held together by one or more disulfide bonds...
  67. ncbi Cloning and characterization of a cDNA encoding murine coagulation factor X
    Z Liang
    Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Indiana 46556, USA
    Thromb Haemost 80:87-91. 1998
    The cDNA encoding murine coagulation factor X (fX) was isolated and reconstructed from a lambdaZap cDNA library generated from murine liver mRNA. The cDNA contains 1486 bases starting at the 5'-translation initiation codon...
  68. pmc Structure and dynamics of zymogen human blood coagulation factor X
    Divi Venkateswarlu
    Department of Chemistry, Venable Hall, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biophys J 82:1190-206. 2002
    The solution structure and dynamics of the human coagulation factor X (FX) have been investigated to understand the key structural elements in the zymogenic form that participates in the activation process...
  69. ncbi Beriplex P/N reverses severe warfarin-induced overanticoagulation immediately and completely in patients presenting with major bleeding
    G Evans
    Department of Haematology, Addenbrooke's Hospital, Cambridge University Hospitals NHS Trust, Cambridge CB2 2QQ, UK
    Br J Haematol 115:998-1001. 2001
    ..The median INR before treatment was greater than 20 and, after treatment, 1.1. All patients had a satisfactory clinical response with immediate cessation of bleeding, and no thromboembolic complications occurred...
  70. ncbi In utero transplantation of wild-type fetal liver cells rescues factor X-deficient mice from fatal neonatal bleeding diatheses
    E D Rosen
    W M Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
    J Thromb Haemost 1:19-27. 2003
    b>Factor X (FX)-deficient embryos suffer partial embryonic lethality with approximately 30% of the embryos arresting at midgestation. The remaining animals survive to term but die perinatally mainly from abdominal or intracranial hemorrhage...
  71. ncbi Prothrombin complex concentrate (Beriplex P/N) for emergency anticoagulation reversal: a prospective multinational clinical trial
    I Pabinger
    Department of Internal Medicine, Division of Haematology and Haemostaseology, Medical University Vienna, Vienna, Austria
    J Thromb Haemost 6:622-31. 2008
    ..OBJECTIVES. To determine the effectiveness and safety of emergency OAT reversal by a balanced pasteurized nanofiltered PCC (Beriplex P/N) containing coagulation factors II, VII, IX, and X, and anticoagulant proteins C and S...
  72. pmc A viable mouse model of factor X deficiency provides evidence for maternal transfer of factor X
    S J Tai
    Division of Hematology, The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    J Thromb Haemost 6:339-45. 2008
    Activated factor X (FXa) is a vitamin K-dependent serine protease that plays a pivotal role in blood coagulation by converting prothrombin to thrombin...
  73. ncbi The influence of acetaldehyde and glycosaminoglycans upon factor Xa- and factor X-deficient plasma
    Arthur S Brecher
    Department of Chemistry, Bowling Green State University, OH 43403, USA
    Can J Physiol Pharmacol 80:879-86. 2002
    ..effects of glycosaminoglycans and acetaldehyde (AcH)--glycosaminoglycan (GAG) mixtures upon Factor Xa- (FXa) and Factor X-deficient plasma (FXDP) have been studied by activated partial thromboplastin time (APTT) studies. Heparin at 0...
  74. ncbi The assembly of the factor X-activating complex on activated human platelets
    S S Ahmad
    The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Thromb Haemost 1:48-59. 2003
    Platelet membranes provide procoagulant surfaces for the assembly and expression of the factor X-activating complex and promote the proteolytic activation and assembly of the prothrombinase complex resulting in normal hemostasis...
  75. ncbi Multiple vitamin K-dependent coagulation zymogens promote adenovirus-mediated gene delivery to hepatocytes
    Alan L Parker
    British Heart Foundation Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, 126 University Place, University of Glasgow, G12 8TA, United Kingdom
    Blood 108:2554-61. 2006
    ..Our results indicate a common and pivotal role for distinct vitamin K-dependent coagulation factors in mediating hepatocyte transduction by adenoviruses in vitro and in vivo...
  76. ncbi Blockade of tissue factor-factor X binding attenuates sepsis-induced respiratory and renal failure
    Karen E Welty-Wolf
    Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Physiol Lung Cell Mol Physiol 290:L21-31. 2006
    ..We hypothesized that blockade of factor X binding to the tissue factor-factor VIIa complex would prevent sepsis-induced damage to the lungs and other ..
  77. ncbi The recruitment of blood coagulation factor X into snake venom gland as a toxin: the role of promoter cis-elements in its expression
    Shiyang Kwong
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    Thromb Haemost 102:469-78. 2009
    ..Trocarin D is hypothesised to have evolved from its factor X counterpart (TrFX) through gene duplication and recruitment...
  78. pmc Redundant and synergistic mechanisms control the sequestration of blood-born adenovirus in the liver
    Nelson C Di Paolo
    Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington, USA
    Mol Ther 17:675-84. 2009
    ..Therefore, our study is the first demonstration of the principal possibility of ablating the sequestration of blood-born Ad in the liver via specific inactivation of a defined set of mechanisms that control this process...
  79. pmc Functional role of O-linked and N-linked glycosylation sites present on the activation peptide of factor X
    L Yang
    Edward A Doisy Department of Biochemistry, Saint Louis University School of Medicine, Saint Louis, MO 63104, USA
    J Thromb Haemost 7:1696-702. 2009
    There are two O-linked and two N-linked glycosylation sites on the activation peptide of factor X (FX) involving residues Thr-17, Thr-29, Asn-39 and Asn-49.
  80. ncbi The role of the factor X activation peptide: a deletion mutagenesis approach
    Amy E Rudolph
    Department of Pathology, Division of Laboratory Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Thromb Haemost 88:756-62. 2002
    To understand the role of the factor X (fX) activation peptide (AP), a deletion mutagenesis approach was employed...
  81. ncbi Snake venom activators of factor X: an overview
    G Tans
    Cardiovascular Research Institute Maastricht CARIM, Maastricht University, PO Box 616, NL 6200 MD Maastricht, The Netherlands
    Haemostasis 31:225-33. 2001
    Activators of blood coagulation factor X have been described in the venom of many snake species belonging to the genus Viperidae and Crotalidae as well as from a few Elapid species...
  82. pmc Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell's viper venom-factor X activator (RVV-X)
    Montamas Suntravat
    Program of Medical Microbiology, Faculty of Graduate School, Chulalongkorn University, Bangkok 10330, Thailand
    Toxicon 56:544-53. 2010
    ..b>Factor X activator activity was also measured in 28 venoms, and D. r. siamensis venom was 5-6 times higher than those of B...
  83. ncbi Factor X activator from Vipera lebetina snake venom, molecular characterization and substrate specificity
    E Siigur
    National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia
    Biochim Biophys Acta 1568:90-8. 2001
    ..We showed that V. lebetina venom contains factor X activator (VLFXA) and factor V activator, fibrinolytic enzymes...
  84. ncbi Congenital deficiency of vitamin K-dependent coagulation factors associated with central nervous system anomalies
    John Puetz
    Hemophilia Treatment Center, 1465 S Grand, St Louis Missouri, 63104, USA
    Thromb Haemost 91:819-21. 2004
  85. ncbi Role of the N-terminal epidermal growth factor-like domain of factor X/Xa
    Farooqahmed S Kittur
    Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 279:24189-96. 2004
    The functional importance of the N-terminal epidermal growth factor-like domain (EGF-N) of factor X/Xa (FX/Xa) was investigated by constructing an FX mutant in which the exon coding for EGF-N was deleted from FX cDNA...
  86. ncbi Warfarin-reversal: results of a phase III study with pasteurised, nanofiltrated prothrombin complex concentrate
    Ingrid Pabinger-Fasching
    Haematology and Haemostaseology Division, Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria
    Thromb Res 122:S19-22. 2008
    ..In conclusion, Beriplex P/N is an effective and well-tolerated therapy for rapid, complete and predictable reversal of anticoagulation in patients with acute bleeding or requiring emergency surgery...
  87. ncbi Pharmacokinetics and anticoagulant properties of the factor VIIa-tissue factor inhibitor recombinant Nematode Anticoagulant Protein c2 following subcutaneous administration in man. Dependence on the stoichiometric binding to circulating factor X
    George P Vlasuk
    Corvas International, Inc, 3030 Science Park Road, San Diego, California, 92121, USA
    Thromb Haemost 90:803-12. 2003
    ..the factor VIIa/tissue factor (fVIIa/TF) complex that requires the prerequisite binding to zymogen or activated factor X (fX)...
  88. ncbi A novel therapeutic approach combining human plasma-derived Factors VIIa and X for haemophiliacs with inhibitors: evidence of a higher thrombin generation rate in vitro and more sustained haemostatic activity in vivo than obtained with Factor VIIa alone
    K Tomokiyo
    The Chemo Sero Therapeutic Research Institute, Kaketsuken, Okubo, Kumamoto, Japan
    Vox Sang 85:290-9. 2003
    ..of plasma-derived FVIIa with other blood coagulation factors, and demonstrated that a therapeutic approach combining plasma-derived FVIIa and Factor X (FX) was more useful for treating haemophiliacs with inhibitors than FVIIa alone.
  89. ncbi Association of pharmacokinetic (CYP2C9) and pharmacodynamic (factors II, VII, IX, and X; proteins S and C; and gamma-glutamyl carboxylase) gene variants with warfarin sensitivity
    Eriko Shikata
    Department of Hospital Pharmacy, Faculty of Medicine, Tottori University, Yonago, Japan
    Blood 103:2630-5. 2004
    ....
  90. ncbi Similar molecular interactions of factor VII and factor VIIa with the tissue factor region that allosterically regulates enzyme activity
    Robert F Kelley
    Departments of Protein Engineering and Physiology, Genentech Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Biochemistry 43:1223-9. 2004
    ..We conclude that the proteolytic domain of both VII and VIIa interacts with this region of sTF in a nearly identical fashion. Therefore, zymogen VII can readily adopt a VIIa-like conformation required for binding to TF...
  91. ncbi Herpes simplex virus type 1-encoded glycoprotein C enhances coagulation factor VIIa activity on the virus
    Michael R Sutherland
    Canadian Blood Services, Research and Development Department, Canada
    Thromb Haemost 92:947-55. 2004
    ..initiator, whose cofactor function is required for physiological factor VIIa (FVIIa)-mediated activation of factor X (FX) to FXa...
  92. ncbi CoagMDB: a database analysis of missense mutations within four conserved domains in five vitamin K-dependent coagulation serine proteases using a text-mining tool
    Rebecca E Saunders
    Department of Biochemistry and Molecular Biology, University College London, London, United Kingdom
    Hum Mutat 29:333-44. 2008
    ..Five coagulation serine proteases-factor VII (F7), factor IX (F9), factor X (F10), protein C (PROC), and thrombin (F2)-exhibit high sequence similarities and all require vitamin K...
  93. ncbi [Attempted suicide with super warfarin]
    P Rodrigo Casanova
    Rev Esp Anestesiol Reanim 52:506-7. 2005
  94. ncbi Pharmacokinetics of Beriplex P/N prothrombin complex concentrate in healthy volunteers
    Helmut Ostermann
    University Hospital Munich Grosshadern, Medical Clinic III, Department of Haematology and Oncology, Ludwig Maximilian University, Munich, Germany
    Thromb Haemost 98:790-7. 2007
    ..Rapid PCC infusion produced prompt sustained increases in coagulation factors and anticoagulant proteins with no clinical evidence of thrombosis or viral transmission...
  95. ncbi Influence of coagulation factor, vitamin K epoxide reductase complex subunit 1, and cytochrome P450 2C9 gene polymorphisms on warfarin dose requirements
    Christina L Aquilante
    Department of Pharmacy Practice, University of Florida College of Pharmacy, Gainesville, FL 32610, USA
    Clin Pharmacol Ther 79:291-302. 2006
    ....
  96. ncbi Hemextin AB complex, a unique anticoagulant protein complex from Hemachatus haemachatus (African Ringhals cobra) venom that inhibits clot initiation and factor VIIa activity
    Yajnavalka Banerjee
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore Singapore 117543
    J Biol Chem 280:42601-11. 2005
    ..Molecular interactions of the hemextin AB complex with FVIIa/TF.FVIIa will provide a new paradigm in the search for anticoagulants that inhibit the initiation of blood coagulation...
  97. ncbi Effects of isoflavones on the coagulation and fibrinolytic system of postmenopausal women
    Danyelle R A Rios
    Department of Clinical, Toxicological and Bromatological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil
    Nutrition 24:120-6. 2008
    ..We evaluated the effects of soy isoflavone supplementation on hemostasis in healthy postmenopausal women...
  98. ncbi Plasma tissue factor plus activated peripheral mononuclear cells activate factors VII and X in cardiac surgical wounds
    Takashi Hattori
    Harrison Department of Surgical Research, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Am Coll Cardiol 46:707-13. 2005
    ..The purpose of this study was to test the hypothesis that activated monocytes with soluble plasma tissue factor (pTF) activate factors VII and X to generate thrombin...
  99. ncbi Prerequisites for recombinant factor VIIa-induced thrombin generation in plasmas deficient in factors VIII, IX or XI
    T Livnat
    The Amalia Biron Research Institute of Thrombosis and Hemostasis, Chaim Sheba Medical Centre, Tel Hashomer, Israel
    J Thromb Haemost 4:192-200. 2006
    ..Tissue factor (TF) and or activated platelets were shown to be essential for the rFVIIa activity...
  100. ncbi Anticoagulant modulation of blood cells and platelet reactivity by garlic oil in experimental diabetes mellitus
    O C Ohaeri
    Department of Biochemistry, Faculty of Medical Sciences, University of Jos, Jos, Nigeria
    Biosci Rep 26:1-6. 2006
    ....
  101. ncbi Traces of factor VIIa modulate thromboplastin sensitivity to factors V, VII, X, and prothrombin
    S A Smith
    Department of Biochemistry, University of Illinois at Urbana Champaign, 506 S Mathews, Urbana, IL 61801, USA
    J Thromb Haemost 4:1553-8. 2006
    ..We hypothesized that trace contamination of tissue-derived thromboplastins with FVII(a) blunts sensitivity to plasma FVII levels...

Research Grants62

  1. Regulation of factor VIII Levels and Activity by Members of LDL Receptor Family
    Dudley K Strickland; Fiscal Year: 2013
    ..This complex activates factor X, the next proenzyme in the coagulation cascade...
  2. PROTEINS OF COAGULATION PATHWAYS
    John H Griffin; Fiscal Year: 2013
    ..Recombinant factor X/IX chimeras will be used to determine apo(a) binding domain on fXa while a panel of apo(a) deletion mutants will ..
  3. Lipid Regulation of Thrombin Generation
    Barry R Lentz; Fiscal Year: 2012
    ..in blood coagulation? Factor IXa with its cofactor VIIIa and factor VIIa with its cofactor tissue factor activate factor X to Xa. Factors IXa and VIIa are structurally similar to Xa, as is cofactor VIIIa to Va...
  4. Locus-Specific Imprinting on the Mammalian X Chromosome
    MICHAEL J O'NEILL; Fiscal Year: 2012
    ..X-linked imprinted genes have only recently been discovered, and it is important to gain an understanding of the role these genes play in brain development and how they are regulated. ..
  5. INTERACTION OF PLATELETS WITH COAGULATION FACTORS IX & X
    Peter Walsh; Fiscal Year: 1992
    ..high molecular weight kininogen, and factor Va and promote the proteolytic activation of factor XII, factor XI, factor X, and prothrombin...
  6. METAL DEPENDENT COMPLEXES IN BLOOD COAGULATION
    Barbara Furie; Fiscal Year: 2002
    ..We will determine if the Gla domains of factor X and prothrombin or the EGF domains of factor X interact with factor Va using synthetic peptides with ..
  7. MOLECULAR INTERACTIONS OF BLOOD COAGULATION FACTOR XA
    Katherine High; Fiscal Year: 1999
    Blood coagulation Factor X occupies a central role in the clotting cascade. It can be activated by either Factor VIIa-tissue factor (extrinsic tenase) or by Factor IXa-VIIIa-phospholipid (intrinsic tenase)...
  8. MOLECULAR INTERACTIONS OF BLOOD COAGULATION FACTOR X
    RICHARD PAINTER; Fiscal Year: 1991
    b>Factor X plays a pivotal role in the coagulation scheme with its activation being at the point of convergence of the intrinsic and extrinsic activation pathways leading to the final stages of hemostasis...
  9. SIGNAL PEPTIDASE STRUCTURE AND FUNCTION
    MARK LIVELY; Fiscal Year: 1999
    ..peptide by signal peptidase have been described that have been described that result in hemophilia (coagulation factor X), familial neurohypophyseal diabetes insipidus (vasopressin), and a form of familial hypoparathyroidism (..
  10. Structural studies of blood coagulation proteins
    Barry Stoddard; Fiscal Year: 2006
    ..This complex activates factor X as part of a proteolytic cascade that generates fibrin polymers...
  11. AMPLIFIED IMMUNOASSAY OF BACTERIA AND RECOVERY
    SAGYPASH SADIEV; Fiscal Year: 1999
    ..of bacteria via surface LPS antigens by a modified ELISA using fluoresceinated and Russell s viper venom factor X activator-conjugates (RVV-XA-Ab) and capture on an anti- fluorescein matrix, and detected using a coagulation-..
  12. FUNCTIONS OF ENDOTHELIAL CELL FACTOR IX BINDING PROTEIN
    David Stern; Fiscal Year: 1992
    ..by production of antisera, both monoclonal and polyclonal, to assess its role in kinetic studies of Factor IX and Factor X activation, and the binding of radiolabelled Factor IX/IXa to endothelium...
  13. DEFINITION OF FUNCTIONAL SITES ON COAGULATION FACTOR X
    WILLIAM CHURCH; Fiscal Year: 1993
    Both the intrinsic and extrinsic coagulation pathways terminate with the activation of the blood protein Factor X. Activated Factor X in turn forms a supramolecular complex, termed prothrombinase, consisting of an enzyme (Factor X alpha), ..
  14. VITAMIN K-DEPENDENT PROTEINS IN LIVER & ISOLATED CELLS
    Reidar Wallin; Fiscal Year: 1992
    ..Antibodies against rat prothrombin and factor X will be used to identify 14CO2-Labeled protein precursors in an in vitro carboxylation system of Type II cells...
  15. PROTHROMBIN ACTIVATION ON BLOOD MONONUCLEAR CELLS
    PAULA TRACY; Fiscal Year: 1990
    ..to assemble an endogenous prothrombinase through synthesis, secretion and activation of factor V, factor X and prothrombin...
  16. CELLULAR INTERACTIONS OF PLASMA PROTEINS
    Peter Walsh; Fiscal Year: 2001
    ..The information gained from these studies will make possible the rational development of therapeutic interventions designed to treat hemorrhagic syndromes or to inhibit or prevent thrombogenesis in blood vessels. ..
  17. PROCOAGULANT PROPERTIES OF THE SICKLE CELL MEMBRANE
    Jolyon Jesty; Fiscal Year: 1992
    ..using highly sensitive assays for the phospholipid dependence of two major clotting reactions: the activation of factor X by the factor IXa-VIIIa complex, and of prothrombin by the factor Xa-Va complex...
  18. Factor X: A Cardiac-Specific Transcriptional Regulator
    Charles Ordahl; Fiscal Year: 2006
    ..abstract_text> ..
  19. ULTRASENSITIVE IMMUNOASSAYS FOR CLOTTING FACTORS
    George Doellgast; Fiscal Year: 1991
    ..amplification system based on preparing snake venom conjugates of peptides (specifically Russell's viper venom factor X activator)...
  20. ISOLATION AND ACTION OF ADULT HEPATOCYTE MITOGENS
    Hyam Leffert; Fiscal Year: 1991
    ..Two hypotheses will be tested: (1) Rat plasma Factor X, a coagulation zymogen with a putative homology to the primary sequence of epidermal growth factor ("EGF"), ..
  21. Induction of Tissue Factor by Patient Sera in HUS
    Eric Grabowski; Fiscal Year: 2007
    ..These inhibitors include recombinant-TF pathway inhibitor, an antibody directed against activated factor X, recombinant-activated protein C, and active site-inhibited r-factor VIIa...
  22. ANALYSIS OF FACTOR VIII COAGULANT ANTIGEN
    Mark Weinstein; Fiscal Year: 1990
    ..2) Examine VIII:CAg proteolytically modified by thrombin, plasmin, activated protein C, factor IXa, factor X, and calcium activated protease...
  23. Molecular Recognition in Factor VIIa Induced Coagulation
    S Bajaj; Fiscal Year: 2005
    This application is based upon the conviction that activation of factor X by the tissue factor/factor VIIa (TF/VIIa) complex represents a key step in the initiation of coagulation by the extrinsic pathway...
  24. MEMBRANE PROTEINS IN BLOOD COAGULATION
    Barbara Furie; Fiscal Year: 2003
    ..Factor VIII binds to membranes and the role of a peptide based upon the structure of Factor VIII to accelerate Factor X activation by Factor IXa....
  25. FACTOR X ACTIVATION BY THE EXTRINSIC PATHWAY
    Sriram Krishnaswamy; Fiscal Year: 2006
    b>Factor X activation by the extrinsic pathway is pivotal for the initiation of blood coagulation...
  26. Effect of Mood on Impulsivity Among Adolescent Smokers
    Chad Gwaltney; Fiscal Year: 2005
    ..The long-term objective of this research is to improve adolescent smoking prevention and cessation efforts by gaining a better understanding of the mechanisms leading to smoking in this population. ..
  27. MOLECULAR BIOLOGY AND PHYSIOLOGY OF HUMAN FACTOR VII
    Steven Idell; Fiscal Year: 1992
    ..VIIa to compete with near equal affinity for binding to the cofactor and the inhibition of the formed complex by factor X(Xa) and a serum lipoprotein. Factor VIIa may also be inhibited by antithrombin III in the presence of heparin...
  28. INHIBITORS OF THE EXTRINSIC BLOOD COAGULATION PATHWAY
    TERENCE BRUNCK; Fiscal Year: 1991
    ..the formation of the catalytically active binary complex of tissue factor with VIIa and the activation of Factor X. In Phase II, we intend to develop therapeutic molecules based on the active peptides discovered in Phase I...
  29. CONTROL OF TRNA GENE EXPRESSION IN BOMBYX MORI
    KAREN SPRAGUE; Fiscal Year: 1993
    ..Much of our effort will focus on a novel transcription component, Factor X, that our laboratory has recently isolated...
  30. HTS Assay for Discovery:Small Molecule Regulators (RMI)
    Vineet Gupta; Fiscal Year: 2005
    ..recognizes a wide variety of ligands, including the complement fragment iC3b, fibrinogen, blood clotting factor X and CD54 (ICAM-1)...
  31. LUNG LAVAGE PROCOAGULANTS IN LUNG INJURY AND REPAIR
    Steven Idell; Fiscal Year: 1990
    ..A factor X activating procoagulant activity has been found in bronchoalveolar lavage (BAL) from the lungs of patients with ..
  32. TUMOR PROCOAGULANT;ROLE IN METASTASIS
    MOHAN CHELLADURAI; Fiscal Year: 1993
    ..also associated wit normal tissue; (ii) cancer procoagulant, a cysteine proteinase that directly activates factor X and (iii) a platelet aggregating activity/procoagulant activity (PAA/PCA) detected in several tumor cell lines...
  33. COFACTOR & RECEPTOR INTERACTION OF FACTORS IX, X & VII
    DARREL STAFFORD; Fiscal Year: 2000
    ..Kinetic analysis will be used to characterize the factor Ixa-VIIIa interaction. The rate of factor X activation is a measurement which will be made...
  34. Platelet Receptor Mediated Factor X Activation
    Peter Walsh; Fiscal Year: 2005
    ..promote the catalysis of two sequential calcium-dependent reactions in blood coagulation: the activation of factor X (FX) by a complex of FIXa and FVIIIa and the conversion of prothrombin to thrombin by a complex of FXa and FVa...
  35. The Role of Membranes in Thrombus Formation In Viva
    Barbara Furie; Fiscal Year: 2005
    ..of cell membranes is important for the assembly of the coagulation enzyme complexes tenase, the activator of factor X and prothrombinase, the activator of prothrombin on the membrane surface...
  36. Drosophila DNA Replication Origins
    John Tower; Fiscal Year: 2005
    ..Both proteins may interact with or be part of factor X. Finally, novel genetic methods will be used to identify additional trans regulators of amplification including ..
  37. Murine Reagents and Analytical Tools
    RYAN DORFMAN; Fiscal Year: 2006
    ..and characterized a group of nine mouse plasma proteins including the enzymatic forms of both prothrombin and factor X. A total of seven new polyclonal antibodies to murine antigens were generated...
  38. TISSUE FACTOR IN BLOOD COAGULATION
    Yale Nemerson; Fiscal Year: 2002
    ..of tissue factor in complex with the enzyme, factor VIIa and the next protein involved in the coagulation scheme, factor X. These projects involve research laboratories at Mt. Sinai School of Medicine and Yale University...
  39. Coagulation Regulates Inflammation in Acute Lung Injury
    MARTHA CARRAWAY; Fiscal Year: 2004
    ..The TF-VIIa complex activates Factor X and generates thrombin and fibrin in the alveolar and interstitial compartments...
  40. MOLECULAR BIOLOGY OF FACTOR X ACTIVATION
    JOHN LOLLAR; Fiscal Year: 1999
    ..These studies may have a direct impact in the treatment of hemophilia and the development of novel antithrombotic drugs. ..
  41. GENE TRANSFER IN MODELS OF ARTERIAL INJURY
    Robert Simari; Fiscal Year: 2000
    ..and to develop cytotoxic approaches to limit intimal formation, and (3) to examine the role of thrombosis in intimal formation following vascular injury by investigating a factor X gene and a gene that encodes a factor Xa inhibitor.
  42. ENZYMES OF BLOOD COAGULATION AND FIBRINOLYSIS
    William Lawson; Fiscal Year: 1980
    ..Methods for the large-scale isolation of human factor X from Cohn fraction III have been developed, and its conversion to and storage as factor Xa will also be ..
  43. NUTRITIONAL AND HORMONAL REGULATION OF HEPATIC GENES
    HOWARD TOWLE; Fiscal Year: 2009
    ..hepatic nuclear complex composed of the transcription factors ChREBP (ChoRE-Binding Protein) and Mix (Max-like factor X) has been shown to bind to the ChoRE and mediate the effects of elevated glucose...
  44. ADVANCING BIOMEDICAL RESEARCH THROUGH MBRS-SCORE
    ANTONIO COLOM; Fiscal Year: 2003
    ..Professor of Chemistry, will test the hypothesis that N-glycosylation is essential for a naturally expressed Factor VIII:C blood clotting activity, and discrete changes in its N-glycan structure affects the ability to activate Factor X.
  45. Physiologic and Pharmacologic Regulation of Factor IXa
    John Sheehan; Fiscal Year: 2009
    ..Finally, these studies will provide proof of principle for this exosite as a critical therapeutic target, and identify lead compounds for a novel class of antithrombotics. ..
  46. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    Alireza R Rezaie; Fiscal Year: 2010
    ..To test these hypotheses, we propose to investigate the molecular determinants of the specificity of factor X/Xa (fX/fXa) assemblies into the respective activation complexes using wild-type and mutant proteins...
  47. Investigation of protein interactions in intrinsic blood coagulation pathway
    Divi Venkateswarlu; Fiscal Year: 2007
    ..Intrinsic pathway of blood coagulation involves the proteolytic activation of zymogenic factor X by serine protease IXa in the presence of phospholipids and divalent metal ions...
  48. Vascular Biology of Tissue Factor
    Wolfram Ruf; Fiscal Year: 2008
    ..These studies have, and will continue to produce original data and advanced knowledge of the TF driven thrombotic and inflammatory diseases and new routes to reduce the impact of these diseases. ..
  49. PE BIOSYSTEMS VOYAGER DE MALDI-TOF
    Kenneth Mann; Fiscal Year: 2001
    ..The fulfillment of the specific aims described in detail in the twelve research proposals would be facilitated by the addiction of a Voyager-DE MALDI-TOF ..
  50. VASCULAR BIOLOGY IN CANCER
    Thomas Edgington; Fiscal Year: 2006
    ..Weekly seminars and review of data are conducted by each of the faculty members with their group. M.D., Ph.D. and M.D./Ph.D. candidates are recruited and prepared for an independent investigative career. ..
  51. MICROSTRUCTURAL HETEROGENEITY IN MEMBRANES
    Barry R Lentz; Fiscal Year: 2010
    ..form at the point of contact between membranes held in contact by neuronal fusion proteins;and 7] Determine the ability of a neuronal calcium-binding protein (synaptotagmin) to perturb, and trigger fusion between, model membranes brought ..
  52. HUMAN TISSUE FACTOR AND ITS PLASMA INHIBITOR
    George Broze; Fiscal Year: 2001
    ....
  53. Antiidiotype mAb:an Antiangiogenic /Antimetastatic Agent
    FRANCIS MARKLAND; Fiscal Year: 2002
    ..According to the Angiogenesis Foundation, diseases that may be treatable with angiogenesis-based drugs encompass markets representing 20% of the $322 billion global pharmaceutical market. ..
  54. TRANSCRIPTION FACTOR NFATP IN CHONDROGENESIS
    Laurie Glimcher; Fiscal Year: 2004
    ....
  55. ROLE OF SPECIFIC PROTEINS IN SPERMATOGENESIS
    W Kistler; Fiscal Year: 2007
    ..The in vivo relevance of the protein to promoter activation will be shown by ChIP assays, and additional studies will be done to understand the function of this transcriptional transactivator. ..