rhodopsin

Summary

Summary: A photoreceptor protein found in retinal rods. It is a complex formed by the binding of RETINALDEHYDE, the oxidized form of retinol, to the protein OPSIN and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain.

Top Publications

  1. ncbi Millisecond-timescale, genetically targeted optical control of neural activity
    Edward S Boyden
    Department of Bioengineering, Stanford University, 318 Campus Drive West, Stanford, California 94305, USA
    Nat Neurosci 8:1263-8. 2005
  2. pmc Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry
    Alexxai V Kravitz
    Gladstone Institute of Neurological Disease, 1650 Owens St, San Francisco, California 94158, USA
    Nature 466:622-6. 2010
  3. pmc Channelrhodopsin-2, a directly light-gated cation-selective membrane channel
    Georg Nagel
    Max Planck Institut fur Biophysik, Marie Curie Strasse 15, 60439 Frankfurt, Germany
    Proc Natl Acad Sci U S A 100:13940-5. 2003
  4. ncbi Environmental genome shotgun sequencing of the Sargasso Sea
    J Craig Venter
    Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA
    Science 304:66-74. 2004
  5. ncbi Multimodal fast optical interrogation of neural circuitry
    Feng Zhang
    Department of Bioengineering, Stanford University, Stanford, California 94305, USA
    Nature 446:633-9. 2007
  6. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
  7. pmc The subcellular organization of neocortical excitatory connections
    Leopoldo Petreanu
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    Nature 457:1142-5. 2009
  8. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
  9. pmc Parvalbumin-expressing interneurons linearly transform cortical responses to visual stimuli
    Bassam V Atallah
    Computational Neurosciences Graduate Program, University of California San Diego, La Jolla, California 92093 0634, USA
    Neuron 73:159-70. 2012
  10. pmc Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function
    Shengli Zhao
    Department of Neurobiology, Duke University Medical Center, Durham, North Carolina, USA
    Nat Methods 8:745-52. 2011

Detail Information

Publications343 found, 100 shown here

  1. ncbi Millisecond-timescale, genetically targeted optical control of neural activity
    Edward S Boyden
    Department of Bioengineering, Stanford University, 318 Campus Drive West, Stanford, California 94305, USA
    Nat Neurosci 8:1263-8. 2005
    ..This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers...
  2. pmc Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry
    Alexxai V Kravitz
    Gladstone Institute of Neurological Disease, 1650 Owens St, San Francisco, California 94158, USA
    Nature 466:622-6. 2010
    ....
  3. pmc Channelrhodopsin-2, a directly light-gated cation-selective membrane channel
    Georg Nagel
    Max Planck Institut fur Biophysik, Marie Curie Strasse 15, 60439 Frankfurt, Germany
    Proc Natl Acad Sci U S A 100:13940-5. 2003
    ..ChR1 and ChR2, a related microbial-type rhodopsin from C. reinhardtii, were shown to be involved in generation of photocurrents of this green alga...
  4. ncbi Environmental genome shotgun sequencing of the Sargasso Sea
    J Craig Venter
    Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA
    Science 304:66-74. 2004
    ..2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors...
  5. ncbi Multimodal fast optical interrogation of neural circuitry
    Feng Zhang
    Department of Bioengineering, Stanford University, Stanford, California 94305, USA
    Nature 446:633-9. 2007
    ..NpHR and ChR2 form a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits...
  6. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
    ..Key findings from our studies in cell culture were recapitulated in photoreceptors expressing mutant rhodopsin in animal models of retinitis pigmentosa.
  7. pmc The subcellular organization of neocortical excitatory connections
    Leopoldo Petreanu
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    Nature 457:1142-5. 2009
    ..Our experiments reveal high specificity in the subcellular organization of excitatory circuits...
  8. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
    ..Analysis of the N-glycan in rhodopsin expressed by the HEK293S GnTI(-) stable cell line showed it to be Man(5)GlcNAc(2)...
  9. pmc Parvalbumin-expressing interneurons linearly transform cortical responses to visual stimuli
    Bassam V Atallah
    Computational Neurosciences Graduate Program, University of California San Diego, La Jolla, California 92093 0634, USA
    Neuron 73:159-70. 2012
    ..These results indicate that PV cells are ideally suited to modulate cortical gain and establish a causal relationship between a select neuron type and specific computations performed by the cortex during sensory processing...
  10. pmc Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function
    Shengli Zhao
    Department of Neurobiology, Duke University Medical Center, Durham, North Carolina, USA
    Nat Methods 8:745-52. 2011
    ..This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior...
  11. ncbi Crystal structure of rhodopsin: A G protein-coupled receptor
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
    Science 289:739-45. 2000
    ..We determined the structure of rhodopsin from diffraction data extending to 2.8 angstroms resolution...
  12. ncbi Ultrafast optogenetic control
    Lisa A Gunaydin
    Department of Bioengineering, Stanford University, Stanford, California, USA
    Nat Neurosci 13:387-92. 2010
    ....
  13. pmc Structure of an agonist-bound human A2A adenosine receptor
    Fei Xu
    Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 332:322-7. 2011
    ..The results define the molecule UK-432097 as a "conformationally selective agonist" capable of receptor stabilization in a specific active-state configuration...
  14. pmc High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor
    Vadim Cherezov
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 318:1258-65. 2007
    ..Although the location of carazolol in the beta2-adrenergic receptor is very similar to that of retinal in rhodopsin, structural differences in the ligand-binding site and other regions highlight the challenges in using rhodopsin ..
  15. ncbi An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology
    Alexander M Aravanis
    Department of Bioengineering, Stanford University Medical Center, Stanford, CA 94305, USA
    J Neural Eng 4:S143-56. 2007
    ....
  16. pmc Calpain and PARP activation during photoreceptor cell death in P23H and S334ter rhodopsin mutant rats
    Jasvir Kaur
    Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tubingen, Tubingen, Germany
    PLoS ONE 6:e22181. 2011
    ..Many human cases are caused by mutations in the rhodopsin gene...
  17. ncbi Crystal structure of the ligand-free G-protein-coupled receptor opsin
    Jung Hee Park
    Institut für Medizinische Physik und Biophysik CC2, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Nature 454:183-7. 2008
    In the G-protein-coupled receptor (GPCR) rhodopsin, the inactivating ligand 11-cis-retinal is bound in the seven-transmembrane helix (TM) bundle and is cis/trans isomerized by light to form active metarhodopsin II...
  18. pmc Neuron-type-specific signals for reward and punishment in the ventral tegmental area
    Jeremiah Y Cohen
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 482:85-8. 2012
    ..These results show that VTA GABAergic neurons signal expected reward, a key variable for dopaminergic neurons to calculate reward prediction error...
  19. pmc Driving fast-spiking cells induces gamma rhythm and controls sensory responses
    Jessica A Cardin
    McGovern Institute for Brain Research and Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts 02139, USA
    Nature 459:663-7. 2009
    ..Our data directly support the fast-spiking-gamma hypothesis and provide the first causal evidence that distinct network activity states can be induced in vivo by cell-type-specific activation...
  20. pmc High-efficiency channelrhodopsins for fast neuronal stimulation at low light levels
    Andre Berndt
    Experimental Biophysics, Humboldt Universitat zu Berlin, D 10115 Berlin, Germany
    Proc Natl Acad Sci U S A 108:7595-600. 2011
    ....
  21. ncbi Neural substrates of awakening probed with optogenetic control of hypocretin neurons
    Antoine R Adamantidis
    Department of Psychiatry and Behavioral Sciences, Stanford University, 701B Welch Road, Palo Alto, California 94304, USA
    Nature 450:420-4. 2007
    ..This study establishes a causal relationship between frequency-dependent activity of a genetically defined neural cell type and a specific mammalian behaviour central to clinical conditions and neurobehavioural physiology...
  22. pmc A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping
    Deniz Atasoy
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    J Neurosci 28:7025-30. 2008
  23. ncbi Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections
    Leopoldo Petreanu
    Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Neurosci 10:663-8. 2007
    ..In both hemispheres the L2/3-to-L5 projection is stronger than the L2/3-to-L2/3 projection. Our results suggest that laminar specificity may be identical for local and long-range cortical projections...
  24. pmc Non-redundant odor coding by sister mitral cells revealed by light addressable glomeruli in the mouse
    Ashesh K Dhawale
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    Nat Neurosci 13:1404-12. 2010
    ....
  25. pmc Characterization of engineered channelrhodopsin variants with improved properties and kinetics
    John Y Lin
    Department of Pharmacology, University of California, San Diego, California, USA
    Biophys J 96:1803-14. 2009
    ..ChEF and ChIEF allow more precise temporal control of depolarization, and can induce action potential trains that more closely resemble natural spiking patterns...
  26. ncbi Ultra light-sensitive and fast neuronal activation with the Ca²+-permeable channelrhodopsin CatCh
    Sonja Kleinlogel
    Max Planck Institute of Biophysics, Department of Biophysical Chemistry, Frankfurt am Main, Germany
    Nat Neurosci 14:513-8. 2011
    ..This paves the way for clinical use of light-gated channels...
  27. pmc Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration
    Laura Steindler
    Department of Microbiology, Oregon State University, Corvallis, Oregon, United States of America
    PLoS ONE 6:e19725. 2011
    ..These findings show that PR is a vital adaptation that supports Cand. P. ubique metabolism during carbon starvation, a condition that is likely to occur in the extreme conditions of ocean environments...
  28. pmc The structural basis of agonist-induced activation in constitutively active rhodopsin
    Jörg Standfuss
    Paul Scherrer Institut, 5232 Villigen PSI, Switzerland
    Nature 471:656-60. 2011
    ..Consequently, these structures correspond to receptors in their inactive states. The visual pigment rhodopsin is the only GPCR for which structures exist that are thought to be in the active state...
  29. pmc High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice
    H Wang
    Department of Neurobiology, Duke University, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 104:8143-8. 2007
    ..We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals...
  30. pmc Excitatory transmission from the amygdala to nucleus accumbens facilitates reward seeking
    Garret D Stuber
    Department of Psychiatry, UNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nature 475:377-80. 2011
    ..Thus, optogenetic manipulation of anatomically distinct synaptic inputs to the NAc reveals functionally distinct properties of these inputs in controlling reward-seeking behaviours...
  31. ncbi Crystal structure of metarhodopsin II
    Hui Woog Choe
    Institut für Medizinische Physik und Biophysik CC2, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Nature 471:651-5. 2011
    ..The photoreceptor rhodopsin couples to transducin and bears its ligand 11-cis-retinal covalently bound via a protonated Schiff base to the ..
  32. pmc Global and local fMRI signals driven by neurons defined optogenetically by type and wiring
    Jin Hyung Lee
    Department of Electrical Engineering, Psychiatry and Biobehavioral Sciences, Bioengineering, and Radiology, University of California, Los Angeles, California 90095, USA
    Nature 465:788-92. 2010
    ....
  33. ncbi Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures
    Feng Zhang
    Department of Bioengineering, Stanford University, Stanford, California, USA
    Nat Protoc 5:439-56. 2010
    ..Together, these methods may help in providing circuit-level insight into the dynamics underlying complex mammalian behaviors in health and disease...
  34. ncbi Transgenic mice with a rhodopsin mutation (Pro23His): a mouse model of autosomal dominant retinitis pigmentosa
    J E Olsson
    Berman Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114
    Neuron 9:815-30. 1992
    ..a mutant or wild-type allele from a patient with retinitis pigmentosa and a missense mutation (P23H) in the rhodopsin gene...
  35. pmc Ectopic expression of a microbial-type rhodopsin restores visual responses in mice with photoreceptor degeneration
    Anding Bi
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Neuron 50:23-33. 2006
    ..Using delivery by an adeno-associated viral vector, here, we show that long-term expression of a microbial-type rhodopsin, channelrhodopsin-2 (ChR2), can be achieved in rodent inner retinal neurons in vivo...
  36. pmc The cell biology of vision
    Ching Hwa Sung
    Dyson Vision Research Institute, Department of Ophthalmology, Weill Cornell Medical College, New York, NY 10065, USA
    J Cell Biol 190:953-63. 2010
    ..Studies of genes linked to human inherited blindness have been crucial to understanding the biogenesis of the outer segment and membrane-trafficking of photoreceptors...
  37. ncbi Crystal structure of opsin in its G-protein-interacting conformation
    Patrick Scheerer
    Institut für Medizinische Physik und Biophysik CC2, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Nature 455:497-502. 2008
    Opsin, the ligand-free form of the G-protein-coupled receptor rhodopsin, at low pH adopts a conformationally distinct, active G-protein-binding state known as Ops*...
  38. ncbi A G protein-coupled receptor at work: the rhodopsin model
    Klaus Peter Hofmann
    Institut für Medizinische Physik und Biophysik CC2, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Trends Biochem Sci 34:540-52. 2009
    ..Activation of the model GPCR, rhodopsin, is triggered by photoisomerization of its retinal ligand...
  39. pmc Accumulation of rhodopsin in late endosomes triggers photoreceptor cell degeneration
    Yashodhan Chinchore
    Department of Biology, Dartmouth College, Hanover, New Hampshire, United States of America
    PLoS Genet 5:e1000377. 2009
    ..Previous work using Drosophila as a model system and analysis of specific mutations in human rhodopsin have uncovered a connection between rhodopsin endocytosis and retinal degeneration...
  40. pmc Lateral competition for cortical space by layer-specific horizontal circuits
    Hillel Adesnik
    Howard Hughes Medical Institute, Center for Neural Circuits and Behavior, Neurobiology Section and Department of Neurosciences, University of California San Diego, La Jolla, California 92093 0634, USA
    Nature 464:1155-60. 2010
    ..Through this mechanism, cortical domains exploit horizontal projections to compete for cortical space...
  41. pmc Restoration of visual function in P23H rhodopsin transgenic rats by gene delivery of BiP/Grp78
    Marina S Gorbatyuk
    Department of Molecular Genetics, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 107:5961-6. 2010
    The P23H mutation within the rhodopsin gene (RHO) causes rhodopsin misfolding, endoplasmic reticulum (ER) stress, and activates the unfolded protein response (UPR), leading to rod photoreceptor degeneration and autosomal dominant ..
  42. ncbi Crystal structure of the human beta2 adrenergic G-protein-coupled receptor
    Søren G F Rasmussen
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA
    Nature 450:383-7. 2007
    ..The beta2AR structure differs from rhodopsin in having weaker interactions between the cytoplasmic ends of transmembrane (TM)3 and TM6, involving the ..
  43. pmc Millisecond-timescale optical control of neural dynamics in the nonhuman primate brain
    Xue Han
    Media Lab, Synthetic Neurobiology Group, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Neuron 62:191-8. 2009
    ....
  44. ncbi Apoptosis: final common pathway of photoreceptor death in rd, rds, and rhodopsin mutant mice
    G Q Chang
    Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina 27710
    Neuron 11:595-605. 1993
    Mutations in the retinal degeneration, retinal degeneration slow(/peripherin) and rhodopsin genes cause photoreceptor degeneration in humans and mice...
  45. ncbi The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure
    Tetsuji Okada
    Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, 2 41 6 Aomi, Koto ku, Tokyo 135 0064, Japan
    J Mol Biol 342:571-83. 2004
    A new high-resolution structure is reported for bovine rhodopsin, the visual pigment in rod photoreceptor cells. Substantial improvement of the resolution limit to 2...
  46. pmc Temporal dynamics of neuronal activation by Channelrhodopsin-2 and TRPA1 determine behavioral output in Drosophila larvae
    Stefan R Pulver
    Department of Biology, Brandeis University, National Center of Behavioral Genomics and Volen Center for Complex Systems, Waltham, Massachusetts 02454 9110, USA
    J Neurophysiol 101:3075-88. 2009
    ..Overall, this work suggests that understanding the cellular effects of these genetic tools and their temporal dynamics is important for the design and interpretation of behavioral experiments...
  47. pmc High-resolution labeling and functional manipulation of specific neuron types in mouse brain by Cre-activated viral gene expression
    Sandra J Kuhlman
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
    PLoS ONE 3:e2005. 2008
    ....
  48. ncbi Proteorhodopsins: an array of physiological roles?
    Jed A Fuhrman
    Department of Biological Sciences and Wrigley Institute, University of Southern California, Los Angeles, California 90089 0371, USA
    Nat Rev Microbiol 6:488-94. 2008
    Metagenomic analyses have revealed widespread and diverse retinal-binding rhodopsin proteins (named proteorhodopsins) among numerous marine bacteria and archaea, which has challenged the notion that solar energy can only enter marine ..
  49. pmc AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training
    Yexica Aponte
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA
    Nat Neurosci 14:351-5. 2011
    ..Furthermore, feeding was evoked selectively over drinking without training or prior photostimulus exposure, which suggests that AGRP neurons serve a dedicated role coordinating this complex behavior...
  50. ncbi Evaluation of the rhodopsin knockout mouse as a model of pure cone function
    G B Jaissle
    Department II, University Eye Hospital, Tubingen, Germany
    Invest Ophthalmol Vis Sci 42:506-13. 2001
    To determine a time window in the rhodopsin knockout (Rho(-/-)) mouse during which retinal function is already sufficiently developed but cone degeneration is not yet substantial, thus representing an all-cone retina.
  51. pmc ER stress is involved in T17M rhodopsin-induced retinal degeneration
    Mansi M Kunte
    Department of Cell Biology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas, USA
    Invest Ophthalmol Vis Sci 53:3792-800. 2012
    The human rhodopsin (Rho) mutation T17M leads to autosomal dominant retinitis pigmentosa (adRP)...
  52. ncbi Modeling study of the light stimulation of a neuron cell with channelrhodopsin-2 mutants
    Nir Grossman
    Institute of Biomedical Engineering, Imperial College London, London, UK
    IEEE Trans Biomed Eng 58:1742-51. 2011
    ..The model and the conclusion presented in this study can help to interpret experimental results, design illumination protocols, and seek improvement strategies in the nascent optogenetic field...
  53. pmc The columnar and laminar organization of inhibitory connections to neocortical excitatory cells
    Dennis Kätzel
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    Nat Neurosci 14:100-7. 2011
    ..Although inhibitory connection patterns were stereotypical, the abundance of individual motifs varied between regions and cells, potentially reflecting functional specializations...
  54. pmc Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells
    Bo Xiong
    Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Biol 10:e1001438. 2012
    ..We propose that upon light stimulation, Crag is required for trafficking of Rh from the trans-Golgi network to rhabdomere membranes via a Rab11-dependent vesicular transport...
  55. pmc Proteorhodopsin phototrophy promotes survival of marine bacteria during starvation
    Laura Gomez-Consarnau
    Marine Microbiology, School of Natural Sciences, Linnaeus University, Kalmar, Sweden
    PLoS Biol 8:e1000358. 2010
    ..Thus, proteorhodopsin phototrophy confers a fitness advantage to marine bacteria, representing a novel mechanism for bacterioplankton to endure frequent periods of resource deprivation at the ocean's surface...
  56. pmc A Drosophila metallophosphoesterase mediates deglycosylation of rhodopsin
    Jinguo Cao
    Institute of Life Science, The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, China
    EMBO J 30:3701-13. 2011
    ..During the maturation of Rh1 rhodopsin, a Drosophila light receptor, the oligosaccharide chain is trimmed extensively...
  57. ncbi Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors
    E de Castro
    Department of Biochemistry, University of Geneva, Switzerland
    Biochem Biophys Res Commun 216:133-40. 1995
    Recoverin is a calcium sensor that regulates rhodopsin phosphorylation in a calcium-dependent manner...
  58. pmc Bbs2-null mice have neurosensory deficits, a defect in social dominance, and retinopathy associated with mislocalization of rhodopsin
    Darryl Y Nishimura
    Department of Pediatrics, Division of Medical Genetics, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 101:16588-93. 2004
    ..Photoreceptor cell death is preceded by mislocalization of rhodopsin, indicating a defect in transport...
  59. pmc Iroquois complex genes induce co-expression of rhodopsins in Drosophila
    Esteban O Mazzoni
    Center for Developmental Genetics, Department of Biology, New York University, New York, New York, United States of America
    PLoS Biol 6:e97. 2008
    ..The exclusive expression of one rhodopsin per photoreceptor is a widespread phenomenon, although exceptions exist...
  60. ncbi A point mutation of the rhodopsin gene in one form of retinitis pigmentosa
    T P Dryja
    Howe Laboratory of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114
    Nature 343:364-6. 1990
    ..As the gene coding for rhodopsin is also assigned to the long arm of chromosome 3 and is expressed in rod photoreceptors that are affected early ..
  61. ncbi Mechanisms of cell death in rhodopsin retinitis pigmentosa: implications for therapy
    Hugo F Mendes
    Division of Pathology, Institute of Ophthalmology, University College London, 11 43 Bath Street, London, EC1V 9EL, UK
    Trends Mol Med 11:177-85. 2005
    ..Mutations in rhodopsin are the most common cause of autosomal-dominant RP (ADRP)...
  62. pmc Apoptotic photoreceptor cell death in mouse models of retinitis pigmentosa
    C Portera-Cailliau
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287 9257
    Proc Natl Acad Sci U S A 91:974-8. 1994
    ..Although mutations in the rhodopsin, peripherin, and cGMP phosphodiesterase genes have been identified in some forms of RP, it remains to be ..
  63. ncbi Adaptation in vertebrate photoreceptors
    G L Fain
    Department of Physiological Science, University of California, Los Angeles, California 90095 1527, USA
    Physiol Rev 81:117-151. 2001
    ..light is absorbed within the outer segment of a vertebrate photoreceptor, the conformation of the photopigment rhodopsin is altered to produce an activated photoproduct called metarhodopsin II or Rh(*)...
  64. ncbi Retinopathy induced in mice by targeted disruption of the rhodopsin gene
    M M Humphries
    Wellcome Ocular Genetics Unit, Genetics Department, Trinity College, Dublin, Ireland
    Nat Genet 15:216-9. 1997
    ..Genetic linkage between an autosomal dominant RP locus and rhodopsin, the photoreactive pigment of the rod cells, led to the identification of mutations within the rhodopsin gene in ..
  65. pmc G protein-coupled receptor rhodopsin
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Biochem 75:743-67. 2006
    The rhodopsin crystal structure provides a structural basis for understanding the function of this and other G protein-coupled receptors (GPCRs)...
  66. pmc Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin
    Xiang Li
    Department of Neurosciences, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 102:17816-21. 2005
    ..To develop these tools we demonstrated that two light-activated signaling proteins, vertebrate rat rhodopsin 4 (RO4) and the green algae channelrhodospin 2 (ChR2), could be used to control neuronal excitability and ..
  67. pmc Novel adeno-associated virus serotypes efficiently transduce murine photoreceptors
    Mariacarmela Allocca
    Telethon Institute of Genetics and Medicine, Via P Castellino 111, 80131 Napoli, Italy
    J Virol 81:11372-80. 2007
    ..In addition, following subretinal administration of AAV, the rhodopsin promoter allows significantly higher levels of photoreceptor expression than the other ubiquitous or ..
  68. ncbi The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints
    Robert Fredriksson
    Department of Neuroscience, Biomedical Center, Box 593, 75 124 Uppsala, Sweden
    Mol Pharmacol 63:1256-72. 2003
    ..Our results show, with high bootstrap support, five main families, named glutamate, rhodopsin, adhesion, frizzled/taste2, and secretin, forming the GRAFS classification system...
  69. pmc Identification of an outer segment targeting signal in the COOH terminus of rhodopsin using transgenic Xenopus laevis
    B M Tam
    Program in Neuroscience, Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Cell Biol 151:1369-80. 2000
    Mislocalization of the photopigment rhodopsin may be involved in the pathology of certain inherited retinal degenerative diseases...
  70. pmc A user's guide to channelrhodopsin variants: features, limitations and future developments
    John Y Lin
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Exp Physiol 96:19-25. 2011
    ..Using this information, valuable qualities and deficits of each ChR variant are summarized. Optimal uses and potential future improvements of ChRs as optogenetic tools are also discussed...
  71. pmc Genome analysis of the proteorhodopsin-containing marine bacterium Polaribacter sp. MED152 (Flavobacteria)
    Jose M Gonzalez
    Department of Microbiology and Cell Biology, University of La Laguna, ES 38206 La Laguna, Tenerife, Spain
    Proc Natl Acad Sci U S A 105:8724-9. 2008
    ..The genome will serve as a model to study the cellular and molecular processes in bacteria that express proteorhodopsin, their adaptation to the oceanic environment, and their role in carbon-cycling...
  72. ncbi Proteorhodopsin in the ubiquitous marine bacterium SAR11
    Stephen J Giovannoni
    Department of Microbiology
    Nature 438:82-5. 2005
    ..The gene is expressed by cells grown in either diurnal light or in darkness, and there is no difference between the growth rates or cell yields of cultures grown in light or darkness...
  73. ncbi Optogenetic control of cardiac function
    Aristides B Arrenberg
    Department of Physiology, University of California, San Francisco, CA 94158, USA
    Science 330:971-4. 2010
    ..Our studies combine optogenetics and light-sheet microscopy to reveal the emergence of organ function during development...
  74. pmc Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa
    Claudio Punzo
    Harvard Medical School, Department of Genetics and Howard Hughes Medical Institute, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Neurosci 12:44-52. 2009
    ..These data suggest that the non-autonomous cone death in retinitis pigmentosa could, at least in part, be a result of the starvation of cones...
  75. pmc Light-powering Escherichia coli with proteorhodopsin
    Jessica M Walter
    Department of Physics, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:2408-12. 2007
    ..Proteorhodopsin allows Escherichia coli cells to withstand environmental respiration challenges by harvesting light energy...
  76. pmc Assembling the marine metagenome, one cell at a time
    Tanja Woyke
    DOE Joint Genome Institute, Walnut Creek, California, United States of America
    PLoS ONE 4:e5299. 2009
    ..A combination of single cell genomics and metagenomics enabled us to analyze the genome content, metabolic adaptations, and biogeography of these taxa...
  77. pmc Transgenic mice carrying the dominant rhodopsin mutation P347S: evidence for defective vectorial transport of rhodopsin to the outer segments
    T Li
    Berman Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114, USA
    Proc Natl Acad Sci U S A 93:14176-81. 1996
    To explore the pathogenic mechanism of dominant mutations affecting the carboxyl terminus of rhodopsin that cause retinitis pigmentosa, we generated five lines of transgenic mice carrying the proline-347 to serine (P347S) mutation...
  78. ncbi Phototransduction and retinal degeneration in Drosophila
    Tao Wang
    Department of Biological Chemistry, Center for Sensory Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Pflugers Arch 454:821-47. 2007
    ..Other proteins play important roles either in the formation or maturation of rhodopsin, or in regeneration of phosphatidylinositol 4,5-bisphosphate (PIP2), which is required for the photoresponse...
  79. ncbi Light stimulates growth of proteorhodopsin-containing marine Flavobacteria
    Laura Gomez-Consarnau
    Marine Microbiology, Department of Biology and Environmental Sciences, University of Kalmar, SE 39182 Kalmar, Sweden
    Nature 445:210-3. 2007
    ..Thus, our results show that the phototrophy conferred by proteorhodopsin can provide critical amounts of energy, not only for respiration and maintenance but also for active growth of marine bacterioplankton in their natural environment...
  80. ncbi Rhodopsin plays an essential structural role in Drosophila photoreceptor development
    J P Kumar
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Development 121:4359-70. 1995
    Null mutations of the Drosophila Rh1 rhodopsin gene, ninaE, result in developmental defects in the photosensitive membranes, the rhabdomeres, of compound eye photoreceptors R1-R6...
  81. pmc Reconstitution of gloeobacter rhodopsin with echinenone: role of the 4-keto group
    Sergei P Balashov
    Department of Physiology and Biophysics, University of California, Irvine, California 92697 4560, United States
    Biochemistry 49:9792-9. 2010
    ..light-harvesting antenna to the light-driven proton pump xanthorhodopsin, into a different protein, gloeobacter rhodopsin expressed in Escherichia coli, and demonstrated that it transfers energy to the retinal chromophore [Imasheva, E...
  82. pmc The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi
    Arunkumar Krishnan
    Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden
    PLoS ONE 7:e29817. 2012
    G protein-coupled receptors (GPCRs) in humans are classified into the five main families named Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin according to the GRAFS classification...
  83. pmc Color-tuned channelrhodopsins for multiwavelength optogenetics
    Matthias Prigge
    Institute of Biology, Experimental Biophysics, Humboldt Universitat zu Berlin, 10115 Berlin, Germany
    J Biol Chem 287:31804-12. 2012
    ..This novel panel of channelrhodopsin variants may serve as an important toolkit element for dual-color cell stimulation in neural circuits...
  84. pmc Rhodopsin gene expression determines rod outer segment size and rod cell resistance to a dominant-negative neurodegeneration mutant
    Brandee A Price
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 7:e49889. 2012
    ..We have addressed both questions by performing an in vivo titration with rhodopsin gene copies in genetically engineered mice that express only normal rhodopsin or an autosomal dominant allele, ..
  85. pmc XPORT-dependent transport of TRP and rhodopsin
    Erica E Rosenbaum
    Department of Ophthalmology and Visual Sciences, Department of Genetics, and Eye Research Institute, University of Wisconsin, Madison, WI 53792, USA
    Neuron 72:602-15. 2011
    ..Here, we identify XPORT (exit protein of rhodopsin and TRP) as a critical chaperone for TRP and its G protein-coupled receptor (GPCR), rhodopsin (Rh1)...
  86. pmc Optogenetic interrogation of dopaminergic modulation of the multiple phases of reward-seeking behavior
    Antoine R Adamantidis
    Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California 94305, USA
    J Neurosci 31:10829-35. 2011
    ..Collectively, these data suggest that activation of dopaminergic neurons facilitates the development of positive reinforcement during reward-seeking and behavioral flexibility...
  87. pmc Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery
    Zhiheng Jia
    Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794 8181, USA
    Circ Arrhythm Electrophysiol 4:753-60. 2011
    ..However, functionality of optogenetic tools has not been fully explored outside neuroscience, and a nonviral, nonembryogenesis-based strategy for optogenetics has not been shown before...
  88. pmc Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II
    Xavier Deupi
    Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
    Proc Natl Acad Sci U S A 109:119-24. 2012
    ..Here, we have used the M257Y(6.40) constitutively active mutant of the photoreceptor rhodopsin in combination with the specific binding of a C-terminal fragment from the G protein alpha subunit (GαCT) to ..
  89. pmc A novel six-rhodopsin system in a single archaeon
    Hsu Yuan Fu
    Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan
    J Bacteriol 192:5866-73. 2010
    ..Here, we report a newly discovered six-rhodopsin system in a single archaeon, Haloarcula marismortui, which shows a more diverse absorbance spectral distribution ..
  90. ncbi Mechanism of rhodopsin kinase regulation by recoverin
    Konstantin E Komolov
    Biochemistry Group, Faculty V, IBU, University of Oldenburg, Oldenburg, Germany
    J Neurochem 110:72-9. 2009
    Recoverin is suggested to inhibit rhodopsin kinase (GRK1) at high [Ca(2+)] in the dark state of the photoreceptor cell. Decreasing [Ca(2+)] terminates inhibition and facilitates phosphorylation of illuminated rhodopsin (Rh*)...
  91. ncbi Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase
    Matthew K Higgins
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 281:19426-32. 2006
    Recoverin is a calcium-dependent inhibitor of rhodopsin kinase...
  92. pmc Optogenetic long-term manipulation of behavior and animal development
    Christian Schultheis
    Institute of Biochemistry, Goethe University, Frankfurt, Germany
    PLoS ONE 6:e18766. 2011
    ..Furthermore, photostimulated ASJ neurons could acutely trigger dauer-exit. Thus, slow ChR2s can be employed to long-term photoactivate behavior and to trigger alternative animal development...
  93. pmc AAV delivery of wild-type rhodopsin preserves retinal function in a mouse model of autosomal dominant retinitis pigmentosa
    Haoyu Mao
    Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA
    Hum Gene Ther 22:567-75. 2011
    ..Earlier, a study on mice carrying mutated rhodopsin transgenes on either RHO + / +  or RHO + /- backgrounds suggested that the amount of wild-type ..
  94. pmc ER stress in retinal degeneration in S334ter Rho rats
    Vishal M Shinde
    Department of Cell Biology and Anatomy, University of North Texas Health Science Center, North Texas Eye Research Institute, Fort Worth, Texas, United States of America
    PLoS ONE 7:e33266. 2012
    The S334ter rhodopsin (Rho) rat (line 4) bears the rhodopsin gene with an early termination codon at residue 334 that is a model for several such mutations found in human patients with autosomal dominant retinitis pigmentosa (ADRP)...
  95. ncbi A high-throughput screening method for small-molecule pharmacologic chaperones of misfolded rhodopsin
    Syed M Noorwez
    Department of Ophthalmology, the Charlie Mack Overstreet Laboratories for Retinal Diseases, University of Florida, Gainesville, Florida 32610, USA
    Invest Ophthalmol Vis Sci 49:3224-30. 2008
    Many mutations in rhodopsin, including P23H, result in misfolding and mislocalization of the protein. It has been demonstrated that pharmacologic chaperones are effective in assisting the proper folding and targeting of P23H opsin...
  96. ncbi Different SAR86 subgroups harbour divergent proteorhodopsins
    Gazalah Sabehi
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Environ Microbiol 6:903-10. 2004
    ....
  97. pmc Interlocked feedforward loops control cell-type-specific Rhodopsin expression in the Drosophila eye
    Robert J Johnston
    Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA
    Cell 145:956-68. 2011
    ..for the transcription factor gene defective proventriculus (dve) as a critical node in the network regulating Rhodopsin expression. dve is a shared component of two opposing, interlocked feedforward loops (FFLs)...
  98. ncbi AAV-mediated delivery of ciliary neurotrophic factor prolongs photoreceptor survival in the rhodopsin knockout mouse
    F Q Liang
    F. M. Kirby Center for Molecular Ophthalmology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA
    Mol Ther 3:241-8. 2001
    ..adeno-associated virus (AAV)-mediated delivery of the gene encoding CNTF delays photoreceptor death in the rhodopsin knockout (opsin(-/-)) mouse, an animal model of RP...
  99. ncbi Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin
    James B Ames
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    J Biol Chem 281:37237-45. 2006
    ..of the neuronal calcium sensor branch of the EF-hand superfamily, serves as a calcium sensor that regulates rhodopsin kinase (RK) activity in retinal rod cells...
  100. ncbi The amino terminus of the fourth cytoplasmic loop of rhodopsin modulates rhodopsin-transducin interaction
    E P Marin
    Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 275:1930-6. 2000
    b>Rhodopsin is a seven-transmembrane helix receptor that binds and catalytically activates the heterotrimeric G protein transducin (G(t))...
  101. pmc Morphological, physiological, and biochemical changes in rhodopsin knockout mice
    J Lem
    New England Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 96:736-41. 1999
    ..developed normally, and rods elaborated outer segments of normal size but with half the normal complement of rhodopsin. Photoreceptor cells in these retinas also degenerated but did so over a much slower time course...

Research Grants71

  1. Structural Basis of Rhodopsin Function
    Javier Navarro; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Rhodopsin is the photoreceptor responsible for dim light vision, and represents the paradigm for the large superfamily of G protein-coupled receptors (GPCRs)...
  2. Cre, Dre Dual Lineage Model for Calcium Imaging and Optogenetic Manipulations
    Petr Tvrdik; Fiscal Year: 2013
    ..This technology will make it possible to stimulate, for example, a specific population of neurons with channel rhodopsin and monitor surrounding glia with calcium indicators, and vice versa...
  3. Ceramide Metabolism and Photoreceptor Homeostasis
    Usha R Acharya; Fiscal Year: 2013
    ..pathway is important for viability of photoreceptors, visual signaling through Phospholipase C, and turnover of Rhodopsin 1 in photoreceptors...
  4. A Ribozyme Rescue Strategy for Dry Age-Related Macular Degeneration
    John M Sullivan; Fiscal Year: 2013
    ..The strategy is: 1) reduce rhodopsin (RHO) to constrain ATR formation that results mostly from rod pigment bleaching, and 2) constrain retinoid cycle ..
  5. Melanopsin Signal Transduction Studied by FTIR Spectroscopy
    Kenneth J Rothschild; Fiscal Year: 2012
    ..Remarkably, the properties of melanopsin strongly resemble invertebrate rhodopsin instead of the extensively studied vertebrate "visual" rhodopsins...
  6. Mechanisms of assembly of photoreceptor G protein complexes
    Barry M Willardson; Fiscal Year: 2013
    ..Many severe retinopathies have been linked to misfolding mutations in rhodopsin and other photoreceptor proteins...
  7. Regulation of TRP channels and visual transduction
    Craig Montell; Fiscal Year: 2013
    ..in this proposal are designed to challenge the current view that the only effector pathway for light-activated rhodopsin is a heterotrimeric G-protein/PLC pathway...
  8. Unfolded Protein Response as a Therapeutic Target for ADRP Animal Models
    Marina Gorbatyuk; Fiscal Year: 2013
    ..More than 100 mutations in rhodopsin account for approximately 30% of ADRP cases with varying severity of visual impairment...
  9. Development of Small Heat Shock Proteins as Therapeutic Agents in the Eye
    JONATHAN MARK PETRASH; Fiscal Year: 2010
    ..can suppress formation of insoluble high molecular weight oligomeric complexes initiated by aggregation-prone rhodopsin and lens crystallin mutants...
  10. FTIR STUDY OF SIGNAL TRANSDUCTION IN SENSORY RHODOPSINS
    Kenneth J Rothschild; Fiscal Year: 2010
    ..Examples of SRs include SRI and SRII from archaebacteria, which control phototaxis, Anabaena sensory rhodopsin (ASR) from freshwater cyanobacteria, which functions as photochromic sensors, some forms of proteorhodopsin (PR) ..
  11. MEMBRANE BASIS OF VISUAL EXCITATION
    Michael F Brown; Fiscal Year: 2012
    ..is to characterize the events at the membrane level that yield the triggering of visual signal transduction by rhodopsin. The significance is that rhodopsin is a prototype for G protein-coupled receptors (GPCRs) that are the targets ..
  12. Novel Adeno-Associated Viral Therapy for X-linked Retinitis Pigmentosa
    James M Wilson; Fiscal Year: 2010
    ..the promoter will then be optimized, testing both a constitutive (CMV) promoter and a photoreceptor- specific (rhodopsin) promoter to determine if one promoter has an efficacy advantage over the other...
  13. Jouberin and Nephrocystin in Joubert Syndrome
    Joseph G Gleeson; Fiscal Year: 2013
    ..We have identified essential signaling functions of these genes in kidney homeostasis, rhodopsin transport, Wnt-, small GTPase-, and phosphatidyl inositol signaling using a combination of mouse modeling, cell ..
  14. GENETIC DISSECTION OF PHOTOTRANSDUCTION
    Baruch Minke; Fiscal Year: 2011
    ..Both Drosophila and presumably the light sensitive RGC are characterized by bi-stable rhodopsin that initiates phototranduction with Transient Receptor Potential (TRP) channels as the final target...
  15. Investigation of Gi/o Signaling in the 5HT System
    DAVINA GUTIERREZ; Fiscal Year: 2010
    ..myself, has recently developed a non-invasive technique to control the Gi/o pathway by light using vertebrate rhodopsin. I have created mice that will allow for the neuron-type specific expression of vertebrate rhodopsin in the ..
  16. Investigating Regulation of Integral Membrane Protein Cofactor Biosynthesis using
    Ronald F Peck; Fiscal Year: 2011
    ..b>Rhodopsin is found in the eyes of many animals, including humans, and consists of an opsin membrane protein and a Vitamin ..
  17. The Regulation of Plasma Membrane Estrogen Receptor-a Levels by Endocytosis
    Reymundo Dominguez; Fiscal Year: 2012
    ..They also strongly suggest that the regulation of membrane ER[unreadable] is analogous to the regulation of rhodopsin receptor and other GPCRs...
  18. Structural dissection of the visual signaling network in cone cells
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2013
    ..While rhodopsin comprises ~90% of protein in rod disc membranes, the composition and organization of opsins in cone cells have ..
  19. RPE MESSENGERS, TRANSCRIPTION AND PHOTORECEPTOR RENEWAL
    NICOLAS GUILLERMO BAZAN; Fiscal Year: 2013
    ..Using RPE cell cultures, rhodopsin transgenic rats and the Ccl2/Cx3cr1-deficient mouse we will define counteracting inflammatory and apoptotic ..
  20. Regulation of Drosophila arrestins in light adaptation
    Bih Hwa Shieh; Fiscal Year: 2012
    ..of this proposal is to investigate how phosphorylation modulates arrestin activity to control the output of rhodopsin thereby fine tuning the visual signaling, using Drosophila eye as a model system...
  21. MOLECULAR STUDIES OF RETINAL DEGENERATION IN DROSOPHILA
    Nansi J Colley; Fiscal Year: 2013
    ..aspects of protein biosynthesis and transport: (1) The SNARE proteins and their role in vesicular transport of rhodopsin and other constituents of phototransduction, and (2) the family of mannosidases and their key roles in trimming ..
  22. Functional analysis of the CAMTA Transcription Factors
    Hong Sheng Li; Fiscal Year: 2013
    ..responding to the Ca2+ sensor calmodulin, is indispensable for rapid deactivation of the light-stimulated GPCR rhodopsin in the Drosophila eye...
  23. Acquisition of a Leica TCS SP5 Confocal Microscope For A Multi-User Facility
    Patricia A Conrad; Fiscal Year: 2013
    ..trafficking in rod photoreceptor cells, deficiencies in which lead to blindness, one focuses on the structure of rhodopsin and its role in retinal degeneration, and one examines mutations in sensory hair cells in the inner ear which ..
  24. Retinal degeneration caused by alterations in protein tyrosine O-sulfation
    Muayyad R Al-Ubaidi; Fiscal Year: 2013
    ..phenotype, we plan to backcross the DKO mice to two well-studied RP models representing the P23H mutation in the rhodopsin gene (VPP) and the haploinsufficiency of the retinal degeneration slow (rds+/-) protein...
  25. Endoplasmic Reticulum Stress in Retinal Degeneration
    Jonathan Lin; Fiscal Year: 2013
    ..Over 100 distinct mutations have been identified in the rhodopsin gene that lead to RP...
  26. Molecular basis for the regulation of G protein-coupled receptor kinases
    John J G Tesmer; Fiscal Year: 2012
    ..produced high resolution crystal structures that represent all three GRK subfamilies, including that of GRK1 (rhodopsin kinase), GRK2 (2-adrenergic receptor kinase 1), and GRK6, as well as structures of GRK2 in complex with ..
  27. Mapping the Inputs onto Neocortical Chandelier Cells
    Jason M Tucciarone; Fiscal Year: 2013
    ..To validate the functional connectivity of inputs to ChCs, Channel Rhodopsin Assisted Circuit Mapping (CRACM) will be used to map the long range presynaptic cells in areas such the ..
  28. Advanced Naturally Designed Channelrhodopsins for Photocontrol of Neural Activity
    JOHN LEE SPUDICH; Fiscal Year: 2010
    ..If needed, site-specific mutagenesis and chimera construction guided by our years of experience in microbial rhodopsin structure-function, photochemistry, and spectral tuning will be applied to further optimize the desired ..
  29. Molecular and Physiological Function of the Tubby Gene Family
    Juergen K Naggert; Fiscal Year: 2013
    ..Currently, the molecular function of TULPs is not known. However, accumulation of rhodopsin containing vesicles in the retina of tubby and Tulp1 mutant mice, and interaction with genes and proteins ..
  30. Regulation of Rhodopsin Expression
    Donald J Zack; Fiscal Year: 2012
    ....
  31. Models of Retinal Degeneration
    Matthew M Lavail; Fiscal Year: 2013
    ..serve as genetic controls and with different eye pigmentation and rates of retinal degeneration;3) P23H mutant rhodopsin transgenic rat lines with 3 different rates of degeneration;and 4) S334ter mutant rhodopsin transgenic rat lines ..
  32. Discovery of antibodies that bind G protein-coupled receptors
    Matthew P DeLisa; Fiscal Year: 2012
    ..coli cells using simple clonal selection. Studies here will focus on the following mammalian GPCRs from the rhodopsin family of receptors: adenosine A2A, cannabinoid CB1 and CB2, the long splice variant of dopamine D2 and ..
  33. Noninvasive Visualization and Analysis of Organ Systems using OCT and Digital Fun
    Steven J Pittler; Fiscal Year: 2010
    ..projects (RO1 EY018143, Analysis of rod photoreceptor GARP and cGMP-gated cation channel in retina;RO1 EY019311, Rhodopsin Trafficking and Retinal Degenerations;RO1 EY006109, Quantitative Analysis of Aging Retina;RO1 EY05922, ..
  34. Rhodopsin Gene Correction and Gene Knockout in Rod Cells
    John H Wilson; Fiscal Year: 2013
    ..such therapies by testing key elements of treatment strategies designed to introduce specific changes into the rhodopsin gene in rod photoreceptor cells in mice...
  35. Delivery of Channel Rhodopsin for Optogenetic Control of Neurons in the Cochlea
    Daniel J Lee; Fiscal Year: 2013
    ..We propose that photosensitization of SGCs and insertion of an optical electrode for controlled stimulation of the inner ear may provide the basis for future CI technology based on light. ..
  36. Illuminating the process of rod outer segment morphogenesis
    Yoshikazu Imanishi; Fiscal Year: 2013
    Abstract: Three membrane proteins in the rod photoreceptors, rhodopsin, peripherin/rds and cGMP-gated channels, localize to different sub-membrane compartments of the outer segments...
  37. Assaying the Anthelmintic Potential of a Novel Flatworm-Specific GPCR Family
    Timothy A Day; Fiscal Year: 2013
    ..These receptors constitute the largest subfamily of Rhodopsin family GPCRs in schistosomes, yet their sequences are quite unique...
  38. Role of Endogenous Opiate Systems in Cocaine Relapse after Long-Term Abstinence
    David W Self; Fiscal Year: 2013
    ..in early and late withdrawal using an optogenetic approach combining inducible viral vector-mediated channel-rhodopsin-2 expression in mice expressing Cre recombinase under POMC promoter control...
  39. Novel Functionally-Selective Serotonin 5HT2 Drugs for Amphetamines Abuse/Disorder
    Drake Morgan; Fiscal Year: 2013
    ..human adrenergic [unreadable]2 GPCR, an innovative advance over previous models that used homology to the bovine rhodopsin GPCR...
  40. CREB and Memory Allocation in the Basolateral Amygdala
    THOMAS W ROGERSON; Fiscal Year: 2012
    ..to use Herpes Simplex Viral (HSV) vectors that can manipulate CREB levels in 15-20% of BLA neurons, and Channel Rhodopsin 2 (ChR2) that allows for the specific activation of these neurons...
  41. Central amygdala input circuits control stress-induced anxiety after chronic ETOH
    Garret D Stuber; Fiscal Year: 2013
    ..stress-induced anxiety after CIA exposure, an AAV5-eYFP vector with a CAMKII promoter containing the rhodopsin derivatives halorhodopsin (NpHR3...
  42. Rhodopsin endocytic trafficking and Drosophila visual sensitivity
    Hong Sheng Li; Fiscal Year: 2010
    ..The major light receptor Rh1 rhodopsin in Drosophila eye is a model molecule for genetic characterization of GPCR signaling and regulation...
  43. MULTIPLEX CIS-REGULATORY ANALYSIS IN MAMMALIAN CELLS
    Joseph C Corbo; Fiscal Year: 2012
    ..In Aim 1 we will dissect, at single nucleotide resolution, the photoreceptor- specific Rhodopsin (Rho) promoter...
  44. RECOVERY OF PHOTOTRANSDUCTION
    Ching Kang Jason Chen; Fiscal Year: 2013
    ..This application is built on three novel preliminary findings: 1) rhodopsin kinase may act as a GTPase accelerating protein (GAP) to ensure timely recovery of phototransduction under dim ..
  45. HTS to Identify Novel Chemical Probes for CCR6
    GREGORY PAUL ROTH; Fiscal Year: 2010
    Chemokine receptors are members of one of the largest gene families encoded within the human genome;the class A, rhodopsin-like, G protein coupled receptors (GPCRs)...
  46. The Role of TULP1 in Photoreceptor Cells
    Stephanie A Hagstrom; Fiscal Year: 2013
    ..In tulp1-/- mice, prior to photoreceptor degeneration, rod and cone opsins are mislocalized, and rhodopsin-bearing extracellular vesicles accumulate around the inner segment (IS), indicating that Tulp1 is necessary for ..
  47. Engineering Microbial Rhodopsins as Optical Voltage Sensors
    ADAM EZRA COHEN; Fiscal Year: 2013
    ..Our starting material is a microbial rhodopsin protein called green proteorhodopsin (GPR)...
  48. A Ribozyme Rescue Strategy for Autosomal Dominant Retinitis Pigmentosa
    John M Sullivan; Fiscal Year: 2012
    ..Expected results are knowledge of the minimum amount of WT human rhodopsin that is needed to keep a mammalian rod photoreceptor alive and vital. Aim 3...
  49. Quality control mechanisms against misfolded rhodopsins in Drosophila.
    HYUNG D RYOO; Fiscal Year: 2013
    ..One of the most common causes of Autosomal Dominant Retinitis Pigmentosa (ADRP) are mutations in the rhodopsin gene that disrupt its encoded protein's folding property...
  50. Synaptic Mechanisms of Addiction-Related Behaviors in the Nucleus Accumbens
    BRAD ALAN GRUETER; Fiscal Year: 2012
    ..of in vivo cocaine experience on these specific inputs will be determined using virally expressed channel rhodopsin (ChR2)...
  51. Synaptic Mechanisms of Addiction-Related Behaviors in the Nucleus Accumbens
    BRAD ALAN GRUETER; Fiscal Year: 2013
    ..of in vivo cocaine experience on these specific inputs will be determined using virally expressed channel rhodopsin (ChR2)...
  52. Coordination of Gene Expression in Retinal Development
    KENNETH MITTON; Fiscal Year: 2009
    ..Stringent regulation of the rhodopsin gene offers a useful paradigm of rod-specific gene expression to explore the interactions between activators, ..
  53. Neurochemical Basis of Opiate Addiction
    HORACE LOH; Fiscal Year: 2013
    ..Since the ?-opioid receptor (OPRM1) is a member of the rhodopsin family of G protein-coupled receptors (GPCR), the model for GPCR desensitization involving the G protein-..
  54. Contact site mapping of transient protein-protein complexes at the molecular leve
    Yumei Huang; Fiscal Year: 2012
    ..In phase II, the combination of the above approaches will be tested and refined iteratively using rhodopsin and transducin as a model system...
  55. Genetics and Therapy of Age-related Retinal Dysfunction in A/J Mice
    Tadao Maeda; Fiscal Year: 2013
    ..decline in human vision manifested by dramatic slowing of rod-mediated dark adaptation attributable to delayed rhodopsin regeneration...
  56. RHODOPSIN TRAFFICKING AND PHOTORECEPTOR MEMBRANE RENEWAL
    DUSANKA DANIELLE DERETIC; Fiscal Year: 2013
    ..goal of the proposed research is to elucidate the molecular mechanisms by which mutations in genes that regulate rhodopsin trafficking and photoreceptor membrane renewal lead to retinal diseases...
  57. P23H Rhodopsin Mutant Swine Model of Reninitis Pigmentosa
    Jason W Ross; Fiscal Year: 2012
    ..we produced six male miniature swine (mini-swine) on a NIH cc haplotype carrying a mutant Pro23His (P23H) human rhodopsin transgene at the National Swine Resource Research Center at the University of Missouri-Columbia...
  58. SPECTRAL AND METABOLIC BASIS OF VISUAL RESPONSES
    M Carter Cornwall; Fiscal Year: 2013
    ..mechanistically distinct stages that are associated with dephosphorylation of C-terminal residues on regenerated rhodopsin and/or transducin translocation...
  59. Trasnducin Interactions with Photoreceptor Membranes
    Theodore G Wensel; Fiscal Year: 2013
    ..of retinal rod outer segments, in which a light signal, photon capture by the G-protein coupled receptor (GPCR), rhodopsin, is converted into a highly amplified electrical signal, hyperpolarization of the transmembrane voltage...
  60. Chromophore Effects in Genetically Diverse Forms of Retinal Dystrophy
    Debra A Thompson; Fiscal Year: 2010
    ..Deficits in 11cRAL production or availability impede the folding, trafficking and stability of rhodopsin and cone opsins, thereby contributing to programmed cell death, particularly affecting the cone cells...
  61. Export of Protein in Escherichia coli
    Linda L Randall; Fiscal Year: 2013
    ..system comprising not only the translocon, but also the accessory proteins SecDF/YajC as well as bacterial rhodopsin as a means to generate protonmotive force...
  62. Postnatal Cajal-Retzius neurons as pacemakers of neocortical network activity
    Carlos Portera-Cailliau; Fiscal Year: 2013
    ..will allow us not only to specifically visualize these cells in Layer 1 but also to conditionaly express channel-rhodopsin using viral vectors and a Cre-Lox approach...
  63. Toward a High Resolution Structure of an Olfactory Receptor
    Steven L Youngentob; Fiscal Year: 2010
    ..e. rhodopsin)...
  64. PHYSIOLOGY AND PATHOPHYSIOLOGY OF PHOTORECEPTORS
    TIMOTHY KRAFT; Fiscal Year: 2002
    ..is a final common pathway resulting in loss of vision for many insults to the eye, including many mutations of rhodopsin or other proteins of the phototransduction cascade...
  65. REGULATORY DOMAINS OF G PROTEIN COUPLED RECEPTORS
    Ellen Weiss; Fiscal Year: 2002
    ..b>Rhodopsin, the photoreceptor of the vertebrate rod cell, has been used as a structural model for investigating the ..
  66. RAMAN INVESTIGATIONS OF THE MOLECULAR BASIS OF VISION
    Richard Mathies; Fiscal Year: 2000
    ..We want to understand how rhodopsin shifts the absorption of its 11-cis retinal protonated Schiff base chromophore from 440 nm in solution to 500 nm ..
  67. RHODOPSIN KINASE: REGULATION, FUNCTION AND STRUCTURE
    Krzysztof Palczewski; Fiscal Year: 1993
    Phosphorylation of rhodopsin by specific protein kinase, rhodopsin kinase is a part of the visual transduction system. This phosphorylation following light absorption is one mechanism of shutting off the excitation process...
  68. OPSIN CGMP-ACTIVATED SINGLE CHANNEL ACTIVITY
    JAMES CLACK; Fiscal Year: 1991
    The goal of this project is determine whether vertebrate opsin or rhodopsin can function as a cyclic GMP-activated channel Opsin will be purified, reconstituted into phospholipid vesicles, and single channel activity will be recorded ..
  69. ANALYSIS OF RHODOPSIN-LIKE PHOTORECEPTORS IN ALGAE
    Kenneth Foster; Fiscal Year: 1991
    The objectives of this project are to understand how rhodopsin is activated and how the signals involving rhodopsin function in the photoreceptor cell...
  70. ROLE OF SUGARS IN PHAGOCYTOSIS OF ROD OUTER SEGMENTS
    EDWARD KEAN; Fiscal Year: 1991
    We are investigating the mechanisms involved in the interaction between rhodopsin-containing membranes and the cells of the retinal pigment epithelium (RPE)...
  71. RHODOPSIN TOPOGRAPHY IN THE ROD DISC MEMBRANE
    W Clay Smith; Fiscal Year: 2010
    ..provided by applicant): The process of vision begins with the absorption of light by the visual pigment, rhodopsin. In a carefully choreographed process, rhodopsin transmits this information in the form of a conformational ..