hemocyanin

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Top Publications

  1. ncbi Modulation of the immune response using Rapana thomasiana hemocyanin
    Andrey Tchorbanov
    Institute of Microbiology, Bulgarian Academy of Sciences, Acad G Bonchev Str 26, 1113 Sofia, Bulgaria
    Int Immunopharmacol 8:1033-8. 2008
  2. ncbi Dendritic cell vaccination in combination with anti-CD25 monoclonal antibody treatment: a phase I/II study in metastatic melanoma patients
    Joannes F M Jacobs
    Department of Medical Oncology, Laboratory of Medical Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Cancer Res 16:5067-78. 2010
  3. pmc Cryo-EM structure of a molluscan hemocyanin suggests its allosteric mechanism
    Qinfen Zhang
    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou 510275, China
    Structure 21:604-13. 2013
  4. ncbi Keyhole limpet hemocyanin (KLH): a biomedical review
    J R Harris
    Institute of Zoology, University of Mainz, Germany
    Micron 30:597-623. 1999
  5. ncbi Enhanced structural stability of Concholepas hemocyanin increases its immunogenicity and maintains its non-specific immunostimulatory effects
    Sergio Arancibia
    Fundación Ciencia y Tecnología para Desarrollo, Santiago, Chile
    Eur J Immunol 42:688-99. 2012
  6. ncbi Hemocyanin from shrimp Litopenaeus vannamei shows hemolytic activity
    Yueling Zhang
    Department of Biology, and Marine Biology Institute, College of Science, Shantou University, Shantou 515063, People s Republic of China
    Fish Shellfish Immunol 27:330-5. 2009
  7. ncbi Similar enzyme activation and catalysis in hemocyanins and tyrosinases
    Heinz Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat, 55099 Mainz, Germany
    Gene 398:183-91. 2007
  8. ncbi Difference between hemocyanin subunits from shrimp Penaeus japonicus in anti-WSSV defense
    Kaiyu Lei
    School of Life Science, Xiamen University, Xiamen 361005, PR China
    Dev Comp Immunol 32:808-13. 2008
  9. ncbi Route of administration modulates the induction of dendritic cell vaccine-induced antigen-specific T cells in advanced melanoma patients
    W Joost Lesterhuis
    Department of Medical Oncology, Nijmegen Centre for Molecular Life Sciences, and Laboratory of Medical Immunology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Cancer Res 17:5725-35. 2011
  10. ncbi Tyrosinase/catecholoxidase activity of hemocyanins: structural basis and molecular mechanism
    H Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat Mainz, D 55099 Mainz, Germany
    Trends Biochem Sci 25:392-7. 2000

Detail Information

Publications296 found, 100 shown here

  1. ncbi Modulation of the immune response using Rapana thomasiana hemocyanin
    Andrey Tchorbanov
    Institute of Microbiology, Bulgarian Academy of Sciences, Acad G Bonchev Str 26, 1113 Sofia, Bulgaria
    Int Immunopharmacol 8:1033-8. 2008
    We have investigated the non-specific immunostimulatory and specific immunomodulatory effects of hemocyanin from marine gastropod Rapana thomasiana (RtH)...
  2. ncbi Dendritic cell vaccination in combination with anti-CD25 monoclonal antibody treatment: a phase I/II study in metastatic melanoma patients
    Joannes F M Jacobs
    Department of Medical Oncology, Laboratory of Medical Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Cancer Res 16:5067-78. 2010
    ..In this study we investigated whether transient monoclonal antibody-mediated depletion of CD25(high) regulatory T cells (Treg) is capable of enhancing the immunostimulatory efficacy of dendritic cell vaccines...
  3. pmc Cryo-EM structure of a molluscan hemocyanin suggests its allosteric mechanism
    Qinfen Zhang
    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou 510275, China
    Structure 21:604-13. 2013
    ..Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1) is an 8 MDa oligomer. Each subunit is made up of eight functional units (FUs)...
  4. ncbi Keyhole limpet hemocyanin (KLH): a biomedical review
    J R Harris
    Institute of Zoology, University of Mainz, Germany
    Micron 30:597-623. 1999
    ..In this review we present a broad survey of fundamental scientific and medically applied studies on keyhole limpet hemocyanin (KLH)...
  5. ncbi Enhanced structural stability of Concholepas hemocyanin increases its immunogenicity and maintains its non-specific immunostimulatory effects
    Sergio Arancibia
    Fundación Ciencia y Tecnología para Desarrollo, Santiago, Chile
    Eur J Immunol 42:688-99. 2012
    ..Here, we study the immunological properties of a modified oxidized Concholepas concholepas hemocyanin (Ox-CCH) obtained by the oxidation of its carbohydrates using sodium periodate...
  6. ncbi Hemocyanin from shrimp Litopenaeus vannamei shows hemolytic activity
    Yueling Zhang
    Department of Biology, and Marine Biology Institute, College of Science, Shantou University, Shantou 515063, People s Republic of China
    Fish Shellfish Immunol 27:330-5. 2009
    b>Hemocyanin is an extracellular copper-containing protein present in the hemolymph of both mollusks and arthropods. The traditionally recognized function of hemocyanin is for oxygen transport...
  7. ncbi Similar enzyme activation and catalysis in hemocyanins and tyrosinases
    Heinz Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat, 55099 Mainz, Germany
    Gene 398:183-91. 2007
    ..The molecular basis of the catalytic process is presented based on recent crystal structures of tyrosinase and hemocyanin. Minor conformational differences at the active site seem to decide about whether the active site is only able ..
  8. ncbi Difference between hemocyanin subunits from shrimp Penaeus japonicus in anti-WSSV defense
    Kaiyu Lei
    School of Life Science, Xiamen University, Xiamen 361005, PR China
    Dev Comp Immunol 32:808-13. 2008
    b>Hemocyanin, the respiratory protein of arthropods and mollusks, was found to function against bacterial and fungal invasion...
  9. ncbi Route of administration modulates the induction of dendritic cell vaccine-induced antigen-specific T cells in advanced melanoma patients
    W Joost Lesterhuis
    Department of Medical Oncology, Nijmegen Centre for Molecular Life Sciences, and Laboratory of Medical Immunology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Cancer Res 17:5725-35. 2011
    ....
  10. ncbi Tyrosinase/catecholoxidase activity of hemocyanins: structural basis and molecular mechanism
    H Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat Mainz, D 55099 Mainz, Germany
    Trends Biochem Sci 25:392-7. 2000
    The enzymes tyrosinase, catecholoxidase and hemocyanin all share similar active sites, although their physiological functions differ...
  11. ncbi Bacterial tyrosinases
    Harald Claus
    Institute for Microbiology and Wine Research, University of Mainz, Becherweg 15, D 55099 Mainz, Germany
    Syst Appl Microbiol 29:3-14. 2006
    ..This review summarizes the present knowledge of bacterial tyrosinases, which are promising models in order to get more insights in structure, enzymatic reactions and functions of "type 3 copper" proteins in general...
  12. ncbi SIGNATURE: a single-particle selection system for molecular electron microscopy
    James Z Chen
    Howard Hughes Medical Institute, W M Keck Institute for Cellular Visualization, Department of Biochemistry, Brandeis University, Waltham, MA 22454 9110, USA
    J Struct Biol 157:168-73. 2007
    ..The system design emphasizes both functionality and usability. This software has been released to the EM community and has been successfully applied to macromolecular structural analyses...
  13. ncbi Direct injection of protamine-protected mRNA: results of a phase 1/2 vaccination trial in metastatic melanoma patients
    Benjamin Weide
    Department of Dermatology, Division of Dermatooncology, University of Tubingen, Tubingen, Germany
    J Immunother 32:498-507. 2009
    ..In 10 patients keyhole limpet hemocyanin (KLH) was added to the vaccine. Granulocyte macrophage colony-stimulating factor was applied as an adjuvant...
  14. ncbi In vitro anticancer effects of a novel immunostimulant: keyhole limpet hemocyanin
    Dale R Riggs
    Department of Surgery, West Virginia University, PO Box 9238, Morgantown, 26506, USA
    J Surg Res 108:279-84. 2002
    Keyhole limpet hemocyanin (KLH) is a recently described immune stimulant and hapten carrier derived from a circulating glycoprotein of the marine mollusk Megathura crenulata...
  15. ncbi Automatic particle selection: results of a comparative study
    Yuanxin Zhu
    Center for Integrative Molecular Biosciences and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 145:3-14. 2004
    ..The dataset consisted of 82 defocus pairs of high-magnification micrographs, containing keyhole limpet hemocyanin particles, acquired using cryoEM...
  16. ncbi Immunodominant role of CCHA subunit of Concholepas hemocyanin is associated with unique biochemical properties
    María Inés Becker
    Fundación Ciencia y Tecnología para el Desarrollo, Avenida Eduardo Castillo Velasco 2902, Santiago, Chile
    Int Immunopharmacol 9:330-9. 2009
    b>Hemocyanin, the oxygen transporter metallo-glycoprotein from mollusks, shows strong relationship between its notable structural features and intrinsic immunomodulatory effects...
  17. ncbi Immunomonitoring tumor-specific T cells in delayed-type hypersensitivity skin biopsies after dendritic cell vaccination correlates with clinical outcome
    I Jolanda M de Vries
    Department of Tumor Immunology, Radbond University Nijmegen Medical Center, Geert Grooteplein 26 28, 6500 HB Nijmegen, Netherlands
    J Clin Oncol 23:5779-87. 2005
    ..In this study, we investigated the predictive value of the presence or absence of antigen-specific T cells in biopsies from delayed-type hypersensitivity (DTH) sites...
  18. ncbi Spectroscopic properties and conformational stability of Concholepas concholepas hemocyanin
    Krassimira Idakieva
    Institute of Organic Chemistry, Bulgarian Academy of Sciences, Akad G Bonchev Str Bl 9, Sofia, Bulgaria
    J Fluoresc 18:715-25. 2008
    The structure in solution and conformational stability of the hemocyanin from the Chilean gastropod mollusk Concholepas concholepas (CCH) and its structural subunits, CCH-A and CCH-B, were studied using fluorescence spectroscopy and ..
  19. ncbi Endogenous glucocorticoids play a positive regulatory role in the anti-keyhole limpet hemocyanin in vivo antibody response
    M Fleshner
    Department of Kinesiology and Applied Physiology, University of Colorado, Boulder, CO 80309, USA
    J Immunol 166:3813-9. 2001
    ..Rats were immunized with keyhole limpet hemocyanin (KLH), and blood samples were taken...
  20. ncbi Keyhole limpet hemocyanin: an effective adjunct against melanoma in vivo
    Irfan Rizvi
    Department of Surgery, Robert C Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, USA
    Am J Surg 194:628-32. 2007
    We have previously demonstrated the potent in vitro antiproliferative effects of keyhole limpet hemocyanin (KLH) against melanoma...
  21. ncbi Keyhole limpet hemocyanin induces the activation and maturation of human dendritic cells through the involvement of mannose receptor
    Pietro Presicce
    Dipartimento di Scienze e Tecnologie Biomediche, Cattedra di Immunologia, Universita degli Studi di Milano, Italy
    Mol Immunol 45:1136-45. 2008
    Keyhole limpet hemocyanin (KLH) is a xenoantigen largely used in vitro as an immunogen to study primary antigen-specific T cell responses and in vivo as a vaccine component with optimal carrier qualities...
  22. ncbi Keyhole limpet hemocyanin conjugate vaccines against cancer: the Memorial Sloan Kettering experience
    C Musselli
    Memorial Sloan Kettering Cancer Center, Department of Tumor Vaccinology, New York, NY 10021, USA
    J Cancer Res Clin Oncol 127:R20-6. 2001
    ..tumor antigens was best achieved using vaccines containing antigens chemically conjugated to keyhole limpet hemocyanin (KLH) plus a potent immunological adjuvant (QS-21). To date, by using this approach to vaccination...
  23. ncbi The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity
    Markus Perbandt
    Institute of Biochemistry and Molecular Biology I, University Hospital Hamburg Eppendorf, c o DESY, Building 22a, Notkestrasse 85, 22603 Hamburg, Germany
    Biochemistry 42:6341-6. 2003
    Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution...
  24. ncbi Antiviral properties of hemocyanin isolated from shrimp Penaeus monodon
    Xiaobo Zhang
    Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration, 361005, Xiamen, PR China
    Antiviral Res 61:93-9. 2004
    ..coupled with the purified WSSV or a fish iridovirus (Singapore grouper iridovirus, SGIV), and identified as hemocyanin by mass spectrometry...
  25. ncbi Assessing divergence time of Spirulida and Sepiida (Cephalopoda) based on hemocyanin sequences
    Kerstin Martina Warnke
    FR Paläontologie, Freie Universitat Berlin, Berlin, Germany
    Mol Phylogenet Evol 58:390-4. 2011
    The phylogenetic position of the mesopelagic decabrachian cephalopod Spirula is still a matter of debate. Since hemocyanin has successfully been used to calibrate a molecular clock for many molluscan species, a molecular clock was ..
  26. pmc Phase III multicenter clinical trial of the sialyl-TN (STn)-keyhole limpet hemocyanin (KLH) vaccine for metastatic breast cancer
    David Miles
    Mount Vernon Cancer Center, Northwood, Middlesex, United Kingdom
    Oncologist 16:1092-100. 2011
    ..evaluated time to progression (TTP) and overall survival in women with metastatic breast cancer (MBC) who received sialyl-TN (STn) keyhole limpet hemocyanin (KLH) vaccine. Secondary endpoints included vaccine safety and immune response.
  27. ncbi Intracutaneous and intravesical immunotherapy with keyhole limpet hemocyanin compared with intravesical mitomycin in patients with non-muscle-invasive bladder cancer: results from a prospective randomized phase III trial
    Rianne J M Lammers
    CuraTrial Site Management Organisation and Research, Arnhem, The Netherlands
    J Clin Oncol 30:2273-9. 2012
    ..Keyhole limpet hemocyanin (KLH) was beneficial in earlier studies...
  28. ncbi Evolution of molluscan hemocyanin structures
    Jürgen Markl
    Institute of Zoology, Johannes Gutenberg University, Mainz, Germany
    Biochim Biophys Acta 1834:1840-52. 2013
    b>Hemocyanin transports oxygen in the hemolymph of many molluscs and arthropods and is therefore a central physiological factor in these animals...
  29. ncbi Keyhole limpet hemocyanin, a novel immune stimulant with promising anticancer activity in Barrett's esophageal adenocarcinoma
    David W McFadden
    Department of Surgery, Robert C Byrd Health Science Center, West Virginia University, P O Box 9238, Morgantown, WV 26506 9238, USA
    Am J Surg 186:552-5. 2003
    Keyhole limpet hemocyanin (KLH) is a recently described immune stimulant and hapten carrier derived from a circulating glycoprotein of the marine mollusk Megathura crenulata...
  30. ncbi Characterization of hemocyanin from the peacock mantis shrimp Odontodactylus scyllarus (Malacostraca: Hoplocarida)
    Samantha Scherbaum
    Biocenter Grindel, Institute of Zoology and Zoological Museum, University of Hamburg, Martin Luther King Platz 3, 20146, Hamburg, Germany
    J Comp Physiol B 180:1235-45. 2010
    b>Hemocyanin is the blue respiratory protein of many arthropod species...
  31. ncbi Complete sequence of the 24-mer hemocyanin of the tarantula Eurypelma californicum. Structure and intramolecular evolution of the subunits
    R Voit
    Division of Molecular Biology of the Cell II, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    J Biol Chem 275:39339-44. 2000
    Hemocyanins are large oligomeric respiratory proteins found in many arthropods and molluscs. The hemocyanin of the tarantula Eurypelma californicum is a 24-mer protein complex with molecular mass of 1, 726,459 Da that consists of seven ..
  32. ncbi Current and new strategies in immunotherapy for superficial bladder cancer
    Frank G E Perabo
    Department of Urology, University Hospital, Bonn, Germany
    Urology 64:409-21. 2004
  33. ncbi The allosteric effector l-lactate induces a conformational change of 2x6-meric lobster hemocyanin in the oxy state as revealed by small angle x-ray scattering
    H Hartmann
    Institute of Molecular Biophysics, Johannes Gutenberg-University of Mainz, Welder-Weg 26, D-55128 Mainz, Germany
    J Biol Chem 276:19954-8. 2001
    ..of the physiological allosteric effector l-lactate on the oxygenated quaternary structure of the 2x6-meric hemocyanin from the lobster Homarus americanus employing small angle x-ray scattering (SAXS)...
  34. ncbi Molecular evolution of the arthropod hemocyanin superfamily
    T Burmester
    Institute of Zoology, University of Mainz, D 55099 Mainz, Germany
    Mol Biol Evol 18:184-95. 2001
    ..Monte Carlo shuffling approaches provided evidence against a discernible relationship of the arthropod hemocyanin superfamily and molluscan hemocyanins or nonarthropodan tyrosinases...
  35. ncbi T helper 2 biased de novo immune response to Keyhole Limpet Hemocyanin in humans
    D Spazierer
    Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna Competence Center, 1090 Vienna, Austria
    Clin Exp Allergy 39:999-1008. 2009
    ..Allergen-specific T helper 2 (Th2) cells play an important role in the pathogenesis of atopic disorders. To date, no model system exists in humans that would allow the monitoring of a developing de novo Th2 immune response in vivo...
  36. ncbi Small-angle X-ray scattering-based three-dimensional reconstruction of the immunogen KLH1 reveals different oxygen-dependent conformations
    Hermann Hartmann
    Institut für Molekulare Biophysik, Universitat Mainz, D 55099 Mainz, Germany
    J Biol Chem 279:2841-5. 2004
    For decades the respiratory protein keyhole limpet hemocyanin (KLH1) from the marine gastropod Megathura crenulata has been used widely as a potent immunostimulant, useful hapten carrier, and valuable agent in the treatment of bladder ..
  37. ncbi Pilot study of a heptavalent vaccine-keyhole limpet hemocyanin conjugate plus QS21 in patients with epithelial ovarian, fallopian tube, or peritoneal cancer
    Paul J Sabbatini
    Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Clin Cancer Res 13:4170-7. 2007
    To characterize the safety and immunogenicity of a heptavalent antigen-keyhole limpet hemocyanin (KLH) plus QS21 vaccine construct in patients with epithelial ovarian, fallopian tube, or peritoneal cancer in second or greater complete ..
  38. ncbi The occurrence of hemocyanin in Hexapoda
    Christian Pick
    Institute of Zoology and Zoological Museum, University of Hamburg, Hamburg, Germany
    FEBS J 276:1930-41. 2009
    ..However, we recently identified a functional hemocyanin in the stonefly Perla marginata (Plecoptera) and in the firebrat Thermobia domestica (Zygentoma)...
  39. ncbi Hemocyanin suggests a close relationship of Remipedia and Hexapoda
    Beyhan Ertas
    Biozentrum Grindel und Zoologisches Museum, Universitat Hamburg, Hamburg, Germany
    Mol Biol Evol 26:2711-8. 2009
    ..Here we report the sequence of hemocyanin from Speleonectes tulumensis Yager, 1987 (Remipedia, Speleonectidae)...
  40. pmc Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin
    T Kawabata
    Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd, Hyogo, Japan
    Proc Natl Acad Sci U S A 92:7774-8. 1995
    ..The cDNAs of both pro-PO subunits lacked signal peptide sequences. This result supports our contention that mature pro-PO accumulates in the cytoplasm of hemocytes and is released by cell rupture, as for arthropod hemocyanins...
  41. ncbi A phase I trial of tumor lysate-pulsed dendritic cells in the treatment of advanced cancer
    Alfred E Chang
    Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109 0932, USA
    Clin Cancer Res 8:1021-32. 2002
    ..The objectives of this study were to assess the toxicity and immunological response induced by the intradermal (i.d) administration of tumor lysate-pulsed dendritic cells (DCs)...
  42. ncbi Natural humoral immune competence and survival in layers
    L Star
    Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
    Poult Sci 86:1090-9. 2007
    ..competence of the chickens was studied by measuring levels of natural antibodies (NAb) binding to keyhole limpet hemocyanin (KLH) or lipopolysaccharide (LPS), respectively, and hemolytic (classical and alternative) complement activity ..
  43. pmc Structural mechanism of SDS-induced enzyme activity of scorpion hemocyanin revealed by electron cryomicroscopy
    Yao Cong
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 17:749-58. 2009
    ..SDS can convert inactive PO and the oxygen carrier hemocyanin (Hc) into enzymatically active PO...
  44. ncbi Nautilus pompilius hemocyanin: 9 A cryo-EM structure and molecular model reveal the subunit pathway and the interfaces between the 70 functional units
    Christos Gatsogiannis
    Institute of Zoology, Johannes Gutenberg University, D 55099 Mainz, Germany
    J Mol Biol 374:465-86. 2007
    ..Nautilus pompilius (Cephalopoda) hemocyanin is a cylindrical decamer of a 350 kDa polypeptide subunit that in turn is a "pearl-chain" of seven ..
  45. ncbi Keyhole limpet hemocyanin: 9-A CryoEM structure and molecular model of the KLH1 didecamer reveal the interfaces and intricate topology of the 160 functional units
    Christos Gatsogiannis
    Institute of Zoology, Johannes Gutenberg University, D 55099 Mainz, Germany
    J Mol Biol 385:963-83. 2009
    ..Our recently published 9-A cryo-electron microscopy/crystal structure hybrid model of a 3.4-MDa cephalopod hemocyanin decamer [Nautilus pompilius hemocyanin (NpH)] revealed the pathway of the seven-FU subunit (340 kDa), 15 types ..
  46. ncbi Modulation of haemocyanin oxygen affinity: properties and physiological implications in a changing world
    C R Bridges
    Institut fur Zoophysiologie, Lehrstuhl für Stoffwechselphysiologie, Heinrich Heine Universitat Dusseldorf, Germany
    J Exp Biol 204:1021-32. 2001
    ..The physiological implications of all these findings are discussed in the context of maintaining a flexible response to a changing environment...
  47. pmc Are maternal antibodies really that important? Patterns in the immunologic development of altricial passerine house sparrows (Passer domesticus)
    Marisa O King
    School of Biological Sciences, Washington State University, Pullman, Washington, United States of America
    PLoS ONE 5:e9639. 2010
    ....
  48. ncbi Role of a copper-specific metallothionein of the blue crab, Callinectes sapidus, in copper metabolism associated with degradation and synthesis of hemocyanin
    Marius Brouwer
    College of Marine Sciences, Department of Coastal Sciences, University of Southern Mississippi, Ocean Springs, MS 39564, USA
    J Inorg Biochem 88:228-39. 2002
    ..in copper metabolism associated with the synthesis and degradation of the oxygen-binding copper protein, hemocyanin, we (1) cloned and sequenced hemocyanin cDNA, (2) examined interaction of the CuMTs with endoplasmic reticulum (..
  49. ncbi Tarantula hemocyanin shows phenoloxidase activity
    H Decker
    Institute for Molecular Biophysics, University of Mainz, Welder Weg 26, D 55128 Mainz, Germany
    J Biol Chem 273:25889-92. 1998
    ..We report here in contrast that the copper protein hemocyanin of the tarantula Eurypelma californicum exhibits two different functions. These occur at the same active site...
  50. pmc A hemocyanin from the Onychophora and the emergence of respiratory proteins
    Kristina Kusche
    Institute of Zoology, Johannes Gutenberg University Mainz, D 55099 Mainz, Germany
    Proc Natl Acad Sci U S A 99:10545-8. 2002
    ..Here, we show that the hemolymph of the Epiperipatus sp. (Onychophora: Peripatidae) contains an arthropod-type hemocyanin, demonstrating that such protein exists outside the Euarthropoda...
  51. ncbi 10-A cryoEM structure and molecular model of the Myriapod (Scutigera) 6x6mer hemocyanin: understanding a giant oxygen transport protein
    Jürgen Markl
    Institute of Zoology, Johannes Gutenberg University, 55099 Mainz, Germany
    J Mol Biol 392:362-80. 2009
    Oxygen transport in Myriapoda is maintained by a unique 6x6mer hemocyanin, that is, 36 subunits arranged as six hexamers (1x6mers)...
  52. ncbi Complete hemocyanin subunit sequences of the hunting spider Cupiennius salei: recent hemocyanin remodeling in entelegyne spiders
    Pia Ballweber
    Institute of Zoology, Johannes Gutenberg University, D 55099 Mainz, Germany
    J Biol Chem 277:14451-7. 2002
    ..Scorpions and orthognath spiders possess a highly conserved 4 x 6-mer hemocyanin that consists of at least seven distinct subunit types (termed a to g)...
  53. ncbi SDS-induced phenoloxidase activity of hemocyanins from Limulus polyphemus, Eurypelma californicum, and Cancer magister
    H Decker
    Institute for Molecular Biophysics, University of Mainz, D55128 Mainz, Germany
    J Biol Chem 276:17796-9. 2001
    ..In chelicerates, the oxygen carrier hemocyanin seems to function as the phenoloxidase...
  54. ncbi Identification and characterization of keyhole limpet hemocyanin N-glycans mediating cross-reactivity with Schistosoma mansoni
    Hildegard Geyer
    Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D 35392 Giessen, Germany
    J Biol Chem 280:40731-48. 2005
    Keyhole limpet hemocyanin (KLH) of the mollusc Megathura crenulata is known to serologically cross-react with Schistosoma mansoni glycoconjugates in a carbohydrate-dependent manner...
  55. ncbi Molecular cloning, characterization and mRNA expression of copper-binding protein hemocyanin subunit in Chinese mitten crab, Eriocheir sinensis
    Shengming Sun
    School of Life Science, East China Normal University, Shanghai 200062, PR China
    Fish Shellfish Immunol 33:1222-8. 2012
    b>Hemocyanin is a copper-binding protein and plays a crucial role in the physiological processes in crustacean...
  56. ncbi Adoptive transfer of natural antibodies to non-immunized chickens affects subsequent antigen-specific humoral and cellular immune responses
    Aart Lammers
    Adaptation Physiology Group, Department of Animal Sciences, Wageningen Institute of Animal Sciences, Wageningen University, Marijkeweg 40, PG 6709, Wageningen, The Netherlands
    Dev Comp Immunol 28:51-60. 2004
    ..chickens, pooled plasma obtained from non-immunized (naïve) 15 months old hens was subjected to keyhole limpet hemocyanin (KLH) antigen-affinity chromatography...
  57. pmc Recombinant Shiga toxin B-subunit-keyhole limpet hemocyanin conjugate vaccine protects mice from Shigatoxemia
    Paola Marcato
    Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada
    Infect Immun 73:6523-9. 2005
    ..The study revealed that sera from mice immunized with such a preparation, conjugated to keyhole limpet hemocyanin and administered with the Ribi adjuvant system, displayed the highest Shiga toxin 2 B-subunit-specific ..
  58. ncbi Quadrupole time-of-flight mass spectrometry of the native hemocyanin of the deep-sea crab Bythograea thermydron
    Franck Zal
    Equipe Ecophysiologie, UMR 7127 CNRS UPMC, Station Biologique, BP 74, 29682 Roscoff Cedex, France
    Biomacromolecules 3:229-31. 2002
    ..for the first time the structure obtained by a commercial ESI quadrupole time-of-flight spectrometer on a native hemocyanin of deep-sea crab Bythograea thermydron with a molecular mass of 1.3 MDa...
  59. ncbi Comparative modeling of the latent form of a plant catechol oxidase using a molluskan hemocyanin structure
    Carsten Gerdemann
    Anorganisch Chemisches Institut der Universität Münster, Wilhelm Klemm Strasse 8, 48149, Munster, Germany
    J Inorg Biochem 89:155-8. 2002
    ..Struct. Biol. 5 (1998) 1084] and of hemocyanin from giant octopus (Octopus dofleini) [J. Mol. Biol. 278 (1998) 855]...
  60. ncbi Putative phenoloxidases in the tunicate Ciona intestinalis and the origin of the arthropod hemocyanin superfamily
    A Immesberger
    Institute of Zoology, University of Mainz, 55099, Mainz, Germany
    J Comp Physiol B 174:169-80. 2004
    In addition to the respiratory copper-containing proteins for which it is named, the arthropod hemocyanin superfamily also includes phenoloxidases and various copperless storage proteins (pseudo-hemocyanins, hexamerins and hexamerin ..
  61. ncbi Location of intrinsic and inducible phenoloxidase activity in molluscan hemocyanin
    Nurul Islam Siddiqui
    Laboratory of Biochemistry, Chemistry Department, Katholieke Universiteit Leuven, Celestijnenlaan 200 G, 3001 Leuven Heverlee, Belgium
    Biochem Biophys Res Commun 348:1138-44. 2006
    ..The subtilisin treatment of Hp f also induced monoPO activity, considered to be at the origin of browning of the sample...
  62. ncbi Retinyl palmitate does not have an adjuvant effect on the antibody response of chicks to keyhole limpet hemocyanin regardless of vitamin A status
    K A Livingston
    Department of Animal Science, University of California, Davis, CA, USA
    Poult Sci 90:965-70. 2011
    ..At 14 d of age, chicks were challenged with keyhole limpet hemocyanin (KLH), Freund's incomplete adjuvant (FIA), KLH and FIA, KLH and RP administered in the same injection, or KLH ..
  63. ncbi Recent findings on phenoloxidase activity and antimicrobial activity of hemocyanins
    Heinz Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat, 55099 Mainz, Germany
    Dev Comp Immunol 28:673-87. 2004
  64. ncbi Detecting particles in cryo-EM micrographs using learned features
    Satya P Mallick
    Department of Electrical and Computer Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    J Struct Biol 145:52-62. 2004
    ..The method has been evaluated on a publicly available dataset of 82 cryoEM images of keyhole lympet hemocyanin (KLH)...
  65. ncbi Detection of different quantitative trait loci for antibody responses to keyhole lympet hemocyanin and Mycobacterium butyricum in two unrelated populations of laying hens
    M Siwek
    Animal Breeding and Genetics Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
    Poult Sci 82:1845-52. 2003
    Quantitative trait loci involved in the primary antibody response to keyhole lympet hemocyanin (KLH) and Mycobacterium butyricum were detected in two independent populations of laying hens...
  66. ncbi The opioid antagonist naloxone induces a shift from type 2 to type 1 cytokine pattern in BALB/cJ mice
    P Sacerdote
    Department of Pharmacology, University of Milano, Milan, Italy
    Blood 95:2031-6. 2000
    ..BALB/cJ mice were immunized with 50 or 100 microg of the protein antigen keyhole-limpet hemocyanin (KLH), and treated acutely or chronically with the opioid antagonist naloxone...
  67. ncbi Processing of crayfish hemocyanin subunits into phenoloxidase
    So Young Lee
    College of Pharmacy, Pusan National University, Jangjeong Dong, Kumjeong Ku, Busan, Republic of Korea
    Biochem Biophys Res Commun 322:490-6. 2004
    b>Hemocyanin and phenoloxidase are both copper-binding proteins involved in the immune system for a wide range of animal species. In crayfish, these proteins were purified and characterized from plasma and hemocytes, respectively...
  68. ncbi Functional properties of hemocyanin from Oncomelania hupensis, the intermediate host of Schistosoma japonicum
    Daoyi Guo
    Key Laboratory of Organo Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal University, Ganzhou 341000, People s Republic of China
    Exp Parasitol 123:277-81. 2009
    ..Here we report the functional properties of hemocyanin of O...
  69. pmc Evaluation of human antibody responses to keyhole limpet hemocyanin on a carbohydrate microarray
    Oyindasola Oyelaran
    Laboratory of Medicinal Chemistry, National Cancer Institute, Frederick, MD, USA
    Proteomics Clin Appl 4:285-94. 2010
    Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer...
  70. ncbi Keyhole limpet hemocyanin: molecular structure of a potent marine immunoactivator. A review
    J R Harris
    Institute of Zoology, University of Mainz, Germany
    Eur Urol 37:24-33. 2000
    In this short review we present a survey of the available biochemical and electron microscopic data on keyhole limpet hemocyanin (KLH).
  71. ncbi Keyhole limpet hemocyanin immunotherapy of bladder cancer: laboratory and clinical studies
    D L Lamm
    Department of Urology, Robert C Byrd Health Science Center, West Virginia University, Morgantown 26506 9251, USA
    Eur Urol 37:41-4. 2000
    Since the serendipitous observation by Olsson in 1974 that patients immunized with 5 mg of keyhole limpet hemocyanin (KLH) had a marked reduction in recurrence of superficial bladder cancer, multiple laboratory and clinical studies have ..
  72. ncbi Monoclonal antibodies to molluskan hemocyanin from Concholepas concholepas demonstrate common and specific epitopes among subunits
    Harold Oliva
    Department of Research and Development, BIOSONDA Corp, Eduardo Castillo Velasco 2902, Santiago, Chile
    Hybrid Hybridomics 21:365-74. 2002
    We studied the reactivity of mouse monoclonal antibodies (MAbs) against the hemocyanin from the Chilean marine gastropod Concholepas concholepas (CCH)...
  73. ncbi Cops and robbers: putative evolution of copper oxygen-binding proteins
    H Decker
    Institute for Molecular Biophysics, University of Mainz, Germany
    J Exp Biol 203:1777-82. 2000
    ..Recent results reveal that haemocyanin also exhibits phenoloxidase activity. A scenario is proposed for the evolutionary relationships among copper oxygen-binding proteins (COPs)...
  74. ncbi The refined structure of functional unit h of keyhole limpet hemocyanin (KLH1-h) reveals disulfide bridges
    Elmar Jaenicke
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat, Jakob Welder Weg 26, Mainz, Germany
    IUBMB Life 63:183-7. 2011
    ..The overall molecular architecture of arthropod and molluscan hemocyanin is very different, although they possess a similar binuclear type 3 copper center to bind oxygen in a side-on ..
  75. ncbi Identification and agglutination properties of hemocyanin from the mud crab (Scylla serrata)
    Fang Yan
    Department of Biology and Guangdong Provincial Key laboratory of Marine Biotechnology, Shantou University, Shantou 515063, People s Republic of China
    Fish Shellfish Immunol 30:354-60. 2011
    ..b>Hemocyanin is an important non-specific immune protein present in the hemolymph of both mollusks and arthropods...
  76. pmc QUAFIT: a novel method for the quaternary structure determination from small-angle scattering data
    Francesco Spinozzi
    Dipartimento di Scienze della Vita e dell Ambiente, Università Politecnica delle Marche and Consorzio Nazionale Interuniversitario per le Scienze Fisiche della Materia, Ancona, Italy
    Biophys J 103:511-21. 2012
    ..QUAFIT has been tested by studying the structure of a high molecular weight protein, the hemocyanin from Octopus vulgaris, under solution conditions that stabilize the decameric form or induce dissociation into ..
  77. ncbi Complete subunit sequences, structure and evolution of the 6 x 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata
    Kristina Kusche
    Institute of Zoology, Molecular Animal Physiology, University of Mainz, Germany
    Eur J Biochem 270:2860-8. 2003
    ..Here we report the complete molecular structure of the hemocyanin from the common house centipede Scutigera coleoptrata (Myriapoda: Chilopoda), as deduced from 2D-gel ..
  78. ncbi Contribution of the copper ions in the dinuclear active site to the stability of Carcinus aestuarii hemocyanin
    F Spinozzi
    Department of Sciences Applied to Complex Systems, Section of Physical Sciences and INFM, Polytechnic University of Marche, Via Ranieri 65, 60131 Ancona, Italy
    Arch Biochem Biophys 439:42-52. 2005
    We have investigated the effect of copper binding on the structural properties of hemocyanin (Hc)...
  79. ncbi Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata
    Anne Averdam
    Institute of Zoology, Johannes Gutenberg University, Mainz, Germany
    Eur J Biochem 270:3432-9. 2003
    ..Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: ..
  80. ncbi Allosteric models for multimeric proteins: oxygen-linked effector binding in hemocyanin
    Michael A Menze
    Institut fur Zoophysiologie, Heinrich Heine Universitat, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    Biochemistry 44:10328-38. 2005
    In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment...
  81. ncbi Durations of cold stress modulates overall immunity of chicken lines divergently selected for antibody responses
    B N Hangalapura
    Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
    Poult Sci 83:765-75. 2004
    ..Birds were immunized with keyhole limpet hemocyanin (KLH) at -1, +1, +3, +5, or +7 d relative to the end of the CS treatment...
  82. ncbi Hemocyanin conformational changes associated with SDS-induced phenol oxidase activation
    Sharon Baird
    School of Biological and Environmental Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UK
    Biochim Biophys Acta 1774:1380-94. 2007
    ..Similar assay conditions elicit phenoloxidase activity in another type 3 copper protein, namely hemocyanin, which normally functions as an oxygen carrier...
  83. ncbi Cupredoxin-like domains in haemocyanins
    Elmar Jaenicke
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat, Jakob Welder Weg 26, 55128 Mainz, Germany
    Biochem J 426:373-8. 2010
    ..We propose that the cupredoxin-like domain in both haemocyanin types once served to upload copper to the active site of the oxygen-binding domain...
  84. ncbi Thermodynamics of oxygenation-linked proton and lactate binding govern the temperature sensitivity of O2 binding in crustacean (Carcinus maenas) hemocyanin
    Roy E Weber
    Zoophysiology, Department of Biological Sciences, University of Aarhus, CF Møllers Alle 1131, DK 8000 Aarhus, Denmark
    J Exp Biol 211:1057-62. 2008
    ..the aim of understanding the molecular underpinnings of the enormous variation in the temperature sensitivity of hemocyanin-O2 affinity encountered in crustaceans, we measured O2 binding to Carcinus maenas hemocyanin at two temperatures,..
  85. ncbi A quantitative trait locus for a primary antibody response to keyhole limpet hemocyanin on chicken chromosome 14--confirmation and candidate gene approach
    M Siwek
    Department of Animal Biotechnology, University of Technology and Life Sciences, Mazowiecka 28, 85 225 Bydgoszcz, Poland
    Poult Sci 89:1850-7. 2010
    A QTL involved in the primary antibody response toward keyhole limpet hemocyanin (KLH) was detected on chicken chromosome 14 in the experimental population, which was created by crossing commercial White Leghorn and a Polish native ..
  86. pmc Immunization with N-propionyl polysialic acid-KLH conjugate in patients with small cell lung cancer is safe and induces IgM antibodies reactive with SCLC cells and bactericidal against group B meningococci
    Lee M Krug
    Thoracic Oncology Service, Memorial Sloan Kettering Cancer Center, Weill Medical College of Cornell University, 1275 York Ave, New York, NY 10065, USA
    Cancer Immunol Immunother 61:9-18. 2012
    ..a robust antibody response was noted in patients with SCLC after vaccination with 30 μg of keyhole limpet hemocyanin (KLH)-conjugated N-propionylated (NP-) polySA, but peripheral neuropathy and ataxia were detected in several ..
  87. ncbi Quaternary and subunit structure of Calliphora arylphorin as deduced from electron microscopy, electrophoresis, and sequence similarities with arthropod hemocyanin
    J Markl
    , , Federal Republic of Germany
    J Comp Physiol B 162:665-80. 1992
    ..The epitope of monoclonal antibody Ec-7 directed against tarantula (Eurypelma californicum) hemocyanin subunit d and also reactive to Calliphora arylphorin was traced to a highly conserved peptide of 27 amino acids ..
  88. ncbi C-terminal hemocyanin from hemocytes of Penaeus vannamei interacts with ERK1/2 and undergoes serine phosphorylation
    Phattara Orn Havanapan
    Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    J Proteome Res 8:2476-83. 2009
    ..during Taura syndrome virus (TSV) infection, expression and functional proteomics studies were performed on hemocyanin, which is a major abundant protein in shrimp hemocytes...
  89. ncbi Dietary linoleic acid divergently affects immune responsiveness of growing layer hens
    J W Sijben
    Health and Reproduction Group, Wageningen Institute of Animal Sciences, Wageningen Agricultural University, The Netherlands
    Poult Sci 79:1106-15. 2000
    ..LA)- and linolenic acid (LNA)-enriched diets on humoral and in vivo cellular immune responses to keyhole limpet hemocyanin (KLH)-dinitrophenyl (DNP) and Mycobacterium butyricum were studied in growing layer hens...
  90. pmc Conformational states of the Rapana thomasiana hemocyanin and its substructures studied by dynamic light scattering and time-resolved fluorescence spectroscopy
    Dessislava Georgieva
    Universitatsklinikum Hamburg Eppendorf, Zentrum für Experimentelle Medizin, Institut fur Biochemie und Molekularbiologie I, Hamburg, Germany
    Biophys J 88:1276-82. 2005
    ..and time-resolved fluorescence measurements show that the oxygenated and apo-forms of the Rapana thomasiana hemocyanin, its structural subunits RtH1 and RtH2, and those of the functional unit RtH2e, exist in different conformations...
  91. ncbi Effects of granulocyte-macrophage colony-stimulating factor and foreign helper protein as immunologic adjuvants on the T-cell response to vaccination with tyrosinase peptides
    Carmen Scheibenbogen
    Medizinische Klinik III, Haematologie, Onkologie und Transfusionsmedizin, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Berlin, Germany
    Int J Cancer 104:188-94. 2003
    ..The combined application of GM-CSF and KLH was associated with early induction of T-cell responses...
  92. pmc Structural basis of the lactate-dependent allosteric regulation of oxygen binding in arthropod hemocyanin
    Shun Hirota
    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    J Biol Chem 285:19338-45. 2010
    b>Hemocyanin (Hc) is an oxygen carrier protein in which oxygen binding is regulated by allosteric effectors such as H(+) and L-lactate...
  93. ncbi Primary structure determination of seven novel N-linked carbohydrate chains derived from hemocyanin of Lymnaea stagnalis. 3-O-methyl-D-galactose and N-acetyl-D-galactosamine as constituents of xylose-containing N-linked oligosaccharides in an animal glyco
    J A van Kuik
    Department of Bio Organic Chemistry, Transitorium III, Utrecht University, The Netherlands
    Eur J Biochem 169:399-411. 1987
    b>Hemocyanin from the freshwater snail Lymnaea stagnalis is a high-molecular-mass copper-containing glyco-protein which functions as oxygen carrier in the hemolymph...
  94. ncbi Functional adaptations of oxygen-transport proteins
    N B Terwilliger
    Oregon Institute of Marine Biology, University of Oregon, Charleston, OR 97420, USA
    J Exp Biol 201:1085-98. 1998
    ..Understanding the mechanisms of regulation of expression, synthesis and modulator levels is a key focus of current investigations...
  95. ncbi Vaccination of patients with small-cell lung cancer with synthetic fucosyl GM-1 conjugated to keyhole limpet hemocyanin
    Lee M Krug
    Thoracic Oncology Service, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Clin Cancer Res 10:6094-100. 2004
    ..A prior clinical study demonstrated the potent immunogenicity of fucosyl GM-1 derived from bovine thyroid gland, conjugated to keyhole limpet hemocyanin (KLH) and administered with QS-21 adjuvant.
  96. ncbi Detection of keyhole limpet hemocyanin (KLH)-specific immune responses by intracellular cytokine assay in patients vaccinated with idiotype-KLH vaccine
    S S Neelapu
    ETI Branch, NCI at Frederick, MD 21702-1201, USA
    J Cancer Res Clin Oncol 127:R14-9. 2001
    Keyhole limpet hemocyanin (KLH) has been used as an immune potentiator to enhance antigen-specific responses against haptens and weak antigens including self-antigens...
  97. ncbi All hierarchical levels are involved in conformational transitions of the 4 x 6-meric tarantula hemocyanin upon oxygenation
    Hermann Hartmann
    Institut für Molekulare Biophysik, Universitat Mainz, Jakob Welder Weg 26, D 55099 Mainz, Germany
    Biochim Biophys Acta 1601:132-7. 2002
    The respiratory protein of the tarantula Eurypelma californicum is a 4 x 6-meric hemocyanin that binds oxygen with high cooperativity...
  98. ncbi Novel function of QM protein of shrimp (Penaeus japonicus) in regulation of phenol oxidase activity by interaction with hemocyanin
    Jianyang Xu
    Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China
    Cell Physiol Biochem 21:473-80. 2008
    ..The GST pull-down assays showed that the PjQM protein interacted with shrimp hemocyanin and myosin, indicating that the PjQM protein might participate in prophenoloxidation (proPO) activation system ..
  99. ncbi Immune and clinical responses in patients with metastatic melanoma to CD34(+) progenitor-derived dendritic cell vaccine
    J Banchereau
    Baylor Institute for Immunology Research, Dallas, Texas 75204, USA
    Cancer Res 61:6451-8. 2001
    ..MelanA/MART-1, tyrosinase, MAGE-3, and gp100], as well as influenza matrix peptide (Flu-MP) and keyhole limpet hemocyanin (KLH) as control antigens...
  100. ncbi Dissociation kinetics of hemocyanin from Octopus vulgaris
    R Favilla
    Sezione di Biofisica e Biologia Molecolare, Dipartimento di Fisica, INFM, Universita di Parma, Parma, Italy
    Biochim Biophys Acta 1385:115-25. 1998
    The native form of hemocyanin (Hc) from Octopus vulgaris can be completely dissociated, at alkaline pH and in the presence of EDTA, from 49S decamers to 11S monomers...
  101. ncbi Switch between tyrosinase and catecholoxidase activity of scorpion hemocyanin by allosteric effectors
    Dorothea Nillius
    Institut für Molekulare Biophysik der Johannes Gutenberg Universität Mainz, Jakob Welder Weg 26, Mainz, Germany
    FEBS Lett 582:749-54. 2008
    ..results indicate that hemocyanins can also function as phenoloxidases and here we show for the first time that hemocyanin can be converted to phenoloxidase...

Research Grants62

  1. TECHNOLOGY FOR CULTURE OF MEGATHURA CRENULATA FOR KLH
    DAVID SPAULDING; Fiscal Year: 2007
    ..this project is to produce reliable and large-scale supplies of medical grade, quality-controlled Keyhole Limpet Hemocyanin (KLH) products ready for use in an expanding array of cancer vaccines, immunological uses and other biomedical ..
  2. Tyrosinase Model Complexes using Imidazole Ligands
    JOHN BRANNON GARY; Fiscal Year: 2013
    ..activity (tyrosinase, methane monooxygenase, galactose oxidase, cyctochrome c oxidase), and oxygen transport (hemocyanin)...
  3. Spectroscopic Studies of Active Sites in Copper Proteins
    Edward I Solomon; Fiscal Year: 2011
    ..and determination of differences in substrate interaction with the similar coupled binuclear copper sites in hemocyanin, tyrosinase, mutants of tyrosinase associated with oculocutaneous albinism, and catechol oxidase which relate to ..
  4. Generation of monoclonal and polyclonal antibodies to neolacto-series ganglioside
    Amjad A Ilyas; Fiscal Year: 2010
    ..In Specific Aim 1, we will develop mouse monoclonal antibodies using purified LM1 conjugated to keyhole limpet hemocyanin (KLH) as an immunogen...
  5. Predictors of clinical outcome after therapeutic vaccination in follicular lympho
    Sattva S Neelapu; Fiscal Year: 2013
    ..patient-specific tumor-derived idiotype (Id) protein was conjugated with a carrier protein (keyhole-limpet hemocyanin, KLH) and administered together with an adjuvant (granulocyte-macrophage colony stimulating factor, GM-CSF) to ..
  6. Human methamphetamine vaccine: Translational Avante Garde Award
    THOMAS RICHARD KOSTEN; Fiscal Year: 2013
    ..Our R01(DA023898) has identified an optimal carrier protein - Keyhole Limpet Hemocyanin (KLH), which is already approved for human use, and an approved Chinese squalene lipid adjuvant that is better ..
  7. NATIONAL RESOURCE CENTER FOR CEPHALOPODS
    JAI DWIVEDI; Fiscal Year: 2010
    ..Use of cephalopod hemocyanin in cancer treatment represents an exciting and direct role for cephalopods in biomedicine...
  8. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL AND MODEL STUDIES
    Michael Maroney; Fiscal Year: 1999
    ..g. ferredoxins, sulfite reductase, hemerythrin, ribonucleotide reductase, methane monooxygenase), Cu atoms (e.g. hemocyanin, tyrosinase, ceruloplasmin, CuA in cytochrome oxidase), Mn atoms (e.g...
  9. CONSTRUCTION AND TESTING OF A TRIVALENT VACCINE AGAINST NEUROBLASTOMA ADMINISTERE
    Govind Ragupathi; Fiscal Year: 2012
    ..surface antigens was determined to include chemical conjugation of antigen to carrier molecule keyhole limpet hemocyanin (KLH) and administration with the saponin immunological adjuvant QS-21 (our QS- 21 is termed OPT-821), 2) the ..
  10. COLLAGENS OF HYALINE CARTILAGE
    Richard Mayne; Fiscal Year: 2002
    ..will be prepared using different approaches including i) use of synthetic peptides coupled to keyhole limpet hemocyanin (KLH), ii) adjuvants that heighten the immunogenicity of synthetic peptides, iii) expression of non-collagenous ..
  11. ACTIVE SPECIFIC IMMUNOTHERAPY IN MAN--A MURINE MODEL
    Barry Kahan; Fiscal Year: 1992
    ..butanol-treated or untreated macrophages; c) autoaggregation, homologous cross-linkage, or keyhole limpet hemocyanin hetero-conjugation; and d) bead particle exposure...
  12. NOT STATED
    Marius Brouwer; Fiscal Year: 1992
    ..complex with reduced GSH, which may serve to deliver copper for the biosynthesis of the copper-dependent protein hemocyanin.The presence of three copper-inducible metallothionein genes in the hepatopancreas have also been demonstrated...
  13. IMMUNOLOGY & DISEASES OF THE GASTROINTESTINAL TRACT
    JAMES MC GUIGAN; Fiscal Year: 1991
    ..peptides will be produced by immunization of rabbits with complementary peptides conjugated to keyhole limpet hemocyanin as carrier protein...
  14. NEW APPROACH TO THE REGULATION OF HUMAN T CELL FUNCTION
    William Osborne; Fiscal Year: 1990
    ..Immunological responses in vivo will be monitored by immunization with sheep red blood cells and keyhole limpet hemocyanin, and in vitro by mitogen stimulation and mixed leukocyte culture...
  15. CLINICAL TRIALS WITH A POLYVALENT BREAST CANCER VACCINE
    Philip Livingston; Fiscal Year: 1999
    ..Keyhole limpet hemocyanin (KLH) has been the most potent carrier molecule and the saponin fraction QS-2l the most potent immunological ..
  16. ALVEOLAR MACROPHAGES AND DEFENSE OF THE LUNG IN AIDS
    David Weissman; Fiscal Year: 1991
    ..In vivo pulmonary immune function will be assessed by transbronchoscopic instillation of keyhole limpet hemocyanin (KLH) into a single lingular segment, with subsequent monitoring of specific humoral immune responses in ..
  17. PSMA-KLH Conjugate Vaccines for Prostate Cancer
    Sofija Andjelic; Fiscal Year: 2004
    ..a tumor-associated antigen conjugated to a large and highly immunogenic carrier protein, such as keyhole limpet hemocyanin (KLH) in combination with an effective immunostimulatory adjuvant...
  18. BINDING REACTION THERMOD ANAMICS RESPIRATORY
    Kevin Peters; Fiscal Year: 1992
    ..are characterization of homotropic and heterotropic linkage effects of allosteric proteins (hemoglobin and hemocyanin), ligand regulated aggregation and phase formation , and development of a thermodynamic framework for analysis ..
  19. DENDRITIC CELL MOBILIZATION AND ACTIVE IMMUNOTHERAPY
    Herbert Lyerly; Fiscal Year: 2005
    ..DC lead to a significant circulating T helper response, whereas multiple immunizations with Keyhole Limpet Hemocyanin (KLH) loaded DC were required to generate a detectable circulating T helper response...
  20. De novo design of type 3 copper proteins
    Michael Rosenblatt; Fiscal Year: 2003
    ..e. hemocyanin) and catalytic systems (tyrosinase and catachol oxidase), seen naturally...
  21. COLLAGEN POLYMORPHISM IN NORMAL AND CIRRHOTIC LIVER
    Antonio Martinez Hernandez; Fiscal Year: 1990
    ..known to be specific for cellular fibronectin to generate synthetic peptides that after conjugation with hemocyanin will be used as antigens...
  22. TECHNOLOGY FOR CULTURE OF MEGATHURA CRENULATA FOR KLH
    FRANK OAKES; Fiscal Year: 2003
    ..reproduction and rearing of Megathura crenulata, the Giant Keyhole Limpet, for production of Keyhole Limpet Hemocyanin (KLH), an active, non-specific immunotherapeutic adjuvant widely used in biomedical research and specifically in ..
  23. IMMUNOLOGY MECHANISM OF ANAPHYLAXIS
    Zoltan Ovary; Fiscal Year: 1993
    ..Two T helper cell clones (L3T4) will be used. These clones are specific for keyhole limpet hemocyanin (KLH) and both were already investigated using B cells primed for anti-DNP antibody production...
  24. TECHNOLOGY FOR CULTURE OF MEGATHURA CRENULATA FOR KLH
    FRANK OAKES; Fiscal Year: 2000
    ..Megathura crenulata, the Giant Keyhole Limpet, for use in the production of clinical grade Keyhole Limpet Hemocyanin [KLH], an active, non-specific immunotherapeutic adjuvant used in research and treatment of certain cancers...
  25. EXERCISE PREVENTS THE IMMUNOSUPPRESSIVE EFFECT OF STRESS
    Monika Fleshner; Fiscal Year: 2000
    ..acute stressor exposure on the development of a specific antibody response to a benign protein, keyhole limpet hemocyanin (KLH)...
  26. MODEL INORGANIC SYSTEMS FOR COPPER PROTEINS
    ROBERT GAGNE; Fiscal Year: 1980
    b>Hemocyanin, tyrosinase and laccase are all copper-containing proteins which have a pair of contiguous Cu atoms important for biological activity...
  27. DEVELOPMENTAL ASPECTS OF AN IDIOTYPE VACCINE
    RONALD WARD; Fiscal Year: 1990
    ..We have previously known that a monoclonal antibody, 4C11, coupled to a carrier, Keyhole Limpet hemocyanin, can completely substitute for antigen in inducing protective immunity against a S...
  28. IMMUNIZATION WITH BEC2 ANTIID VACCINE AND GD3-LACTONE
    Paul Chapman; Fiscal Year: 1999
    ..patients against GD3 have been partially successful using either: a) GD3-lactone conjugated to keyhole limpet hemocyanin and mixed with the adjuvant QS21 (GD3-lactone-KLH+QS21) or b) BEC2 anti-idiotypic MAb mixed with the adjuvant ..
  29. MODELS OF LYMPHOCYTE MIGRATION AND LOCALIZATION
    NICHOLAS PONZIO; Fiscal Year: 1980
    ..Passive systemic immunization with homologous keyhole limpet hemocyanin (KLH) antibody followed by local injection of trinitrophenylated (TNP)-KLH in the left front footpad and the non-..
  30. STRUCTURE-BASED DISCOVERY OF CD4-MHC CLASS II INHIBITORS
    MI YOUN LIM; Fiscal Year: 2006
    ..Secondary T cell responses to proteolipid protein (139-151) and keyhole limpet hemocyanin demonstrated that TJU103 also specifically affected the activated CD4+ T cells...
  31. KLH-pulsed dendritic cells plus TNFerade in Pancreatic Cancer
    Emmanuel Zervos; Fiscal Year: 2007
    ..can be induced in patients with pancreatic cancer using autologous dendritic cells pulsed with keyhole limpit hemocyanin after the induction of apoptosis in the primary tumors with an adenoviral vector coding for human tumor necrosis ..
  32. Acute Stress and Macrophage Priming
    Julie Wieseler; Fiscal Year: 2003
    ..The experiments proposed in the second part of the grant involve administering the antigen keyhole limpet hemocyanin (KLH), and then manipulating the availability of NO, ROS or PGE2 and evaluating T-cell proliferation...
  33. Regulation of murine neonatal Th2 function
    REBECCA D ADKINS; Fiscal Year: 2010
    ..This idea is supported by our findings that the function of peripheral CD4+ cells in neonates resembles that of RTE in adults. ..
  34. Anti-CTLA-4 Monoclonal Antibody Therapy for Lymphoma
    John Timmerman; Fiscal Year: 2005
    ..abstract_text> ..
  35. Feline Model of Neuropathy associated with anti-MAG/SGPG antibodies
    AMJAD ILYAS; Fiscal Year: 2007
    ..These studies may lead to the development of a feline model of anti-MAG/SGPG neuropathy that closely resembles the human disorder [unreadable] [unreadable] [unreadable] [unreadable]..
  36. Molecular Determinants of Virulence Porcine Circovirus 2
    Steven Krakowka; Fiscal Year: 2007
    ..Collectively, these data will provide critical insight into pathogenic mechanisms of disease mediated by mammalian circoviruses and will provide key guidance for the future study of human circovirus pathogens. ..
  37. IMMUNE RESPONSES IN PATIENTS WITH GBS AND CIDP
    AMJAD ILYAS; Fiscal Year: 2002
    ..Early and serial serum specimens from GBS and CIDP patients will be examined to see whether autoantibody titers correlate with disease severity. ..
  38. Metal Transfer Reactions between Stable Isotopically Doped Zn Proteins
    Andrew Mason; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  39. NK Cell Differentiation from Stem Cells
    Jeffrey S Miller; Fiscal Year: 2010
    ..A thorough understanding of these mechanisms may allow us to manipulate NK cells for therapeutic purposes to treat cancer. ..
  40. Sjogren's model with anti-Ro/La and Salivary Pathology and Dysfunction
    R Hal Scofield; Fiscal Year: 2010
    ..Improved understanding of the disease at a fundamental level is possible through study of animal models. Such understanding may lead to improved recognition and treatment. ..
  41. Mindfulness to Improve Elders' Immune and Health Status
    Jan Moynihan; Fiscal Year: 2008
    ..The findings of this RCT will have implications for the conceptualization, refinement, and dissemination of psychosocial interventions aimed at slowing the rate of immunosenescence. ..
  42. Stress, Heat-Shock Proteins, and Innate Immunity
    Monika Fleshner; Fiscal Year: 2008
    ..abstract_text> ..
  43. The Rochester Center for Mind-Body Research
    Jan Moynihan; Fiscal Year: 2006
    ..By the end of the award period, we will be a mature, established Center with a submitted program project or Center grant application. ..
  44. EFECTS OF STRESS IN IMMUNOCOMPROMISED HOSTS
    Jan Moynihan; Fiscal Year: 2003
    ..In turn, this will result in prolonged allograft survival. In contrast, the immunocompromised animal will have poorer immune responses to an opportunistic pathogen (HSV-1). ..
  45. Peptide Vaccine for Experimental Autoimmune Myocarditis
    Daniel Zimmerman; Fiscal Year: 2003
    ..The effects of J-My-1 administration on the presence and frequency of this cell type will be evaluated. Our goal is to demonstrate that the severity of EAM can be reduced by J-My-1 administration in animals with EAM. ..
  46. Mapping of transcription network in Th2 cells
    Wei Ping Zheng; Fiscal Year: 2003
    ..The studies in this proposal are extension of the study in our parent grant to isolate molecules associated with GATA-3 to understand the mechanisms of cytokine gene regulation in Th2 cells. ..
  47. Etanercept Therapy in Follicular Lymphoma
    Arnold Freedman; Fiscal Year: 2003
    ..This study is a novel approach to treating FL, where targeting the microenvironment of the tumor may inhibit the growth and survival of the neoplastic cells. ..
  48. Dendritic Cells, Heat Shock Proteins and Tumor Immunity
    Jacques Banchereau; Fiscal Year: 2004
    ..These studies will permit us to produce an altered melanoma cell line, with enhanced immunogenicity, that will be used to generate tumor cell bodies to load onto DCs for vaccination protocols. ..
  49. Immunoregulation by Endogenous Opioids
    Jan Moynihan; Fiscal Year: 2004
    ..Specific Aim 4: To determine if C57B1/6 mice, which do not have altered immune responses to KLH following stress odor exposure, have evidence of elevated endogenous opioids following odor exposure. ..
  50. Pathogenic Mechanisms of Fibrosis:Common Ground
    Arnold Postlethwaite; Fiscal Year: 2004
    ..Young investigator travel awards are being provided to encourage involvement of trainees and other young investigators. ..
  51. Role of inflammation in manganese-induced neurotoxicity
    NIKOLAY FILIPOV; Fiscal Year: 2004
    ..The long-term goal of the proposed studies is to understand the role of microglia and environmental contaminants in neurodegenerative diseases. ..
  52. Mice with Human Dendritic Cells to Test Vaccine Potency
    A Palucka; Fiscal Year: 2004
    ..The experiments proposed here would lay ground for further use of SCID-hu/DCs mice to study other vaccines and demonstrate development of protective immunity upon in vivo rechallenge. ..
  53. Parathyroid and Thymus Transplants in DiGeorge Syndrome
    MARY MARKERT; Fiscal Year: 2005
    ..Indirect allorecognition as a mechanism of graft rejection will be assessed by ELISpot. The third aim will assess safety and tolerability. ..
  54. SCOR on the Pathogenesis of Scleroderma
    Arnold Postlethwaite; Fiscal Year: 2005
    ..abstract_text> ..
  55. HIGH-RESOLUTION ANALYSIS T CELL RESPONSE TO HIV ANTIGENS
    Tim Mosmann; Fiscal Year: 2001
    ..Together, this information should identify the particular immune effector functions that are induced by HIV vaccines or natural HIV infection, and also define the potential for modification of immune responses by therapeutic vaccines. ..