integrin beta3

Summary

Summary: An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.

Top Publications

  1. ncbi Molecular mechanisms of prothrombotic risk due to genetic variations in platelet genes: Enhanced outside-in signaling through the Pro33 variant of integrin beta3
    K Vinod Vijayan
    Department of Medicine, Baylor College of Medicine, One Baylor Plaza, BCM 286, N1319, Houston, TX 77030, USA
    Exp Biol Med (Maywood) 231:505-13. 2006
  2. ncbi Association of sustained ERK activity with integrin beta3 induction during receptor activator of nuclear factor kappaB ligand (RANKL)-directed osteoclast differentiation
    Hong Hee Kim
    National Research Laboratory for Bone Metabolism, Department of Microbiology and Immunology, College of Medicine, Chosun University, Gwangju 501 759, Korea
    Exp Cell Res 289:368-77. 2003
  3. pmc Integrins control motile strategy through a Rho-cofilin pathway
    Erik H J Danen
    Division of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 169:515-26. 2005
  4. ncbi Alpha(v)beta(3) integrin engagement enhances cell invasiveness in human multiple myeloma
    Roberto Ria
    Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy
    Haematologica 87:836-45. 2002
  5. pmc Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption
    Sarah Schmidt
    Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 192:883-97. 2011
  6. pmc Mechanisms of integrin-vascular endothelial growth factor receptor cross-activation in angiogenesis
    Ganapati H Mahabeleshwar
    Department of Molecular Cardiology, The Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Circ Res 101:570-80. 2007
  7. ncbi Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells
    Ronen Zaidel-Bar
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel
    J Cell Sci 116:4605-13. 2003
  8. pmc β3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons
    Karine Pozo
    Medical Research Council Laboratory for Molecular Cell Biology and Cell Biology Unit, University College London, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 109:1323-8. 2012
  9. pmc Tyrosine phosphorylation of integrin beta3 regulates kindlin-2 binding and integrin activation
    Kamila Bledzka
    Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 285:30370-4. 2010
  10. pmc Tyrosine phosphorylation as a conformational switch: a case study of integrin β3 cytoplasmic tail
    Lalit Deshmukh
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, Connecticut 06269, USA
    J Biol Chem 286:40943-53. 2011

Detail Information

Publications246 found, 100 shown here

  1. ncbi Molecular mechanisms of prothrombotic risk due to genetic variations in platelet genes: Enhanced outside-in signaling through the Pro33 variant of integrin beta3
    K Vinod Vijayan
    Department of Medicine, Baylor College of Medicine, One Baylor Plaza, BCM 286, N1319, Houston, TX 77030, USA
    Exp Biol Med (Maywood) 231:505-13. 2006
    ..A large portion of this review will focus on the widely studied Leu33Pro (Pl(A)) polymorphism of integrin beta3, and will consider the potential mechanisms by which the Pro33 polymorphism could induce a prothrombotic risk...
  2. ncbi Association of sustained ERK activity with integrin beta3 induction during receptor activator of nuclear factor kappaB ligand (RANKL)-directed osteoclast differentiation
    Hong Hee Kim
    National Research Laboratory for Bone Metabolism, Department of Microbiology and Immunology, College of Medicine, Chosun University, Gwangju 501 759, Korea
    Exp Cell Res 289:368-77. 2003
    ..undergo osteoclastic differentiation in the presence of RANKL enhanced the RANKL-induced gene expression of integrin beta3 without any effect on the expression of integrin alphav, which was constitutively high...
  3. pmc Integrins control motile strategy through a Rho-cofilin pathway
    Erik H J Danen
    Division of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 169:515-26. 2005
    ..Thus, alterations in integrin expression profiles allow cells to modulate several critical aspects of the motile machinery through Rho GTPases...
  4. ncbi Alpha(v)beta(3) integrin engagement enhances cell invasiveness in human multiple myeloma
    Roberto Ria
    Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy
    Haematologica 87:836-45. 2002
    ..We studied these mechanisms in fresh bone marrow plasma cells of patients with MM after adhesion to the ECM proteins vitronectin (VN) and fibronectin (FN)...
  5. pmc Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption
    Sarah Schmidt
    Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 192:883-97. 2011
    ..These findings show that osteoclasts require their entire integrin repertoire to be regulated by kindlin-3 to orchestrate bone homeostasis...
  6. pmc Mechanisms of integrin-vascular endothelial growth factor receptor cross-activation in angiogenesis
    Ganapati H Mahabeleshwar
    Department of Molecular Cardiology, The Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Circ Res 101:570-80. 2007
    ....
  7. ncbi Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells
    Ronen Zaidel-Bar
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel
    J Cell Sci 116:4605-13. 2003
    ..These observations illuminate, for the first time, early stages in focal complex assembly and the dynamic process associated with its transformation into focal adhesion...
  8. pmc β3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons
    Karine Pozo
    Medical Research Council Laboratory for Molecular Cell Biology and Cell Biology Unit, University College London, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 109:1323-8. 2012
    ..Our findings identify an integrin family member, β3, as a direct interactor of an AMPAR subunit and provide molecular insights into how this cell-adhesion protein regulates the composition of cell-surface AMPARs...
  9. pmc Tyrosine phosphorylation of integrin beta3 regulates kindlin-2 binding and integrin activation
    Kamila Bledzka
    Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 285:30370-4. 2010
    ..These observations indicate that transitions between the phosphorylated and non-phosphorylated states of the integrin β(3) CT determine reactivity with kindlin-2 and govern the role of kindlin-2 in regulating integrin activation...
  10. pmc Tyrosine phosphorylation as a conformational switch: a case study of integrin β3 cytoplasmic tail
    Lalit Deshmukh
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, Connecticut 06269, USA
    J Biol Chem 286:40943-53. 2011
    ..Collectively these data provide unique molecular insights into multiple regulatory roles of phosphorylation...
  11. ncbi Talin binding to integrin beta tails: a final common step in integrin activation
    Seiji Tadokoro
    Department of Cell Biology, The Scripps Research Institute, The Burnham Institute, La Jolla, CA 92037, USA
    Science 302:103-6. 2003
    ..Thus, regulated binding of talin to integrin beta tails is a final common element of cellular signaling cascades that control integrin activation...
  12. pmc Impaired platelet responses to thrombin and collagen in AKT-1-deficient mice
    Juhua Chen
    Joseph J Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic Foundation, NB50, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Blood 104:1703-10. 2004
    ..As a consequence of impaired alpha(IIb)beta(3) activation and platelet aggregation, Akt-1 null mice showed significantly longer bleeding times than wild-type mice...
  13. ncbi Structure of the integrin beta3 transmembrane segment in phospholipid bicelles and detergent micelles
    Tong Lay Lau
    Department of Biochemistry and Molecular Biology and Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, Los Angeles, California 90033, USA
    Biochemistry 47:4008-16. 2008
    ..In the micelle, residues L717-I721 are also embedded but deviate from linear alpha-helical conformation in contrast to I693-K716, which closely resemble the bicelle structure...
  14. pmc A molecular switch that controls cell spreading and retraction
    Panagiotis Flevaris
    Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
    J Cell Biol 179:553-65. 2007
    ..Thus, calpain cleavage of beta(3) at Tyr(759) relieves c-Src-mediated RhoA inhibition, activating the RhoA pathway that confines cell spreading and causes cell retraction...
  15. pmc CD44 and beta3 integrin organize two functionally distinct actin-based domains in osteoclasts
    Anne Chabadel
    Institut de Génomique Fonctionnelle de Lyon, Universite de Lyon, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Ecole Normale Superieure de Lyon, 69364 Lyon, France
    Mol Biol Cell 18:4899-910. 2007
    ..However, although CD44 signals are sufficient to form a SZ, the presence of WIP is indispensable for the formation of a fully functional SZ...
  16. ncbi The Pl(A1/A2) polymorphism of glycoprotein IIIa and cerebrovascular events in hypertension: increased risk of ischemic stroke in high-risk patients
    Francesca Lanni
    Department of Clinical Medicine, Cardiovascular and Immunological Sciences, Federico II University, Naples, Italy
    J Hypertens 25:551-6. 2007
    ..We explored the possibility that this polymorphism represents a risk factor for stroke in hypertensive patients...
  17. ncbi Expression of Integrin beta3 is correlated to the properties of quiescent hemopoietic stem cells possessing the side population phenotype
    Terumasa Umemoto
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    J Immunol 177:7733-9. 2006
    ....
  18. ncbi Identification of a HoxA10 activation domain necessary for transcription of the gene encoding beta3 integrin during myeloid differentiation
    Ling Bei
    Fineberg School of Medicine and the Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 282:16846-59. 2007
    ..Our studies also suggested that HoxA10 is a bifunctional protein that is involved in dynamic regulation of multiple aspects of phagocyte phenotype and function...
  19. pmc Critical cysteine residues for regulation of integrin alphaIIbbeta3 are clustered in the epidermal growth factor domains of the beta3 subunit
    Tetsuji Kamata
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Biochem J 378:1079-82. 2004
    ..It has also been proposed that unique cysteine residues in the integrin beta3 subunit are involved in the regulation of alphaIIbbeta3...
  20. pmc Protein disulfide isomerase capture during thrombus formation in vivo depends on the presence of β3 integrins
    Jaehyung Cho
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Blood 120:647-55. 2012
    ..These results indicate that both endothelial and platelet β3 integrins contribute to extracellular PDI binding at the vascular injury site...
  21. pmc Disruption of the mouse mu-calpain gene reveals an essential role in platelet function
    M Azam
    Section of Hematology Oncology Research, Departments of Medicine, Anatomy, and Cellular Biology, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    Mol Cell Biol 21:2213-20. 2001
    ....
  22. pmc Beta3-integrin-deficient mice are a model for Glanzmann thrombasthenia showing placental defects and reduced survival
    K M Hodivala-Dilke
    Howard Hughes Medical Institute, Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Clin Invest 103:229-38. 1999
    ....
  23. ncbi Vicinal thiols are required for activation of the αIIbβ3 platelet integrin
    N Manickam
    Division of Hematology, Department of Medicine, The University of Texas Health Science Center, San Antonio, TX, USA
    J Thromb Haemost 9:1207-15. 2011
    ..These thiols provide a mechanism to regulate protein function. We previously found that thiols in both αIIb and β3 of the αIIbβ3 fibrinogen receptor were required for platelet aggregation...
  24. ncbi An αIIb mutation in patients with Glanzmann thrombasthenia located in the N-terminus of blade 1 of the β-propeller (Asn2Asp) disrupts a calcium binding site in blade 6
    W Mansour
    Amalia Biron Research Institute of Thrombosis and Hemostasis, Chaim Sheba Medical Center, Tel Hashomer and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
    J Thromb Haemost 9:192-200. 2011
    ..Studies of Glanzmann thrombasthenia (GT)-causing mutations has generated invaluable information on the formation and function of integrin αIIbβ(3)...
  25. ncbi Up-regulation of integrin beta3 expression in porcine vascular endothelial cells cultured in vitro by classical swine fever virus
    Qing hai Tang
    College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi 712100, China
    Vet Immunol Immunopathol 133:237-42. 2010
    ..The cell adhesion molecule, integrin beta3, plays a central role in maintaining and regulating vascular permeability...
  26. pmc Foxc2 transcription factor as a regulator of angiogenesis via induction of integrin beta3 expression
    Hisaki Hayashi
    Division of Cardiovascular Medicine, Vanderbilt University Medical Center, Nashville, TN 37232 6300, USA
    Cell Adh Migr 3:24-6. 2009
    ..One of the key molecules that regulate this process is the integrin beta3 subunit, a cell adhesion receptor that forms a heterodimer with the integrin alphav subunit and interacts with ..
  27. ncbi Transforming growth factor beta1 induces alphavbeta3 integrin expression in human lung fibroblasts via a beta3 integrin-, c-Src-, and p38 MAPK-dependent pathway
    Dmitri V Pechkovsky
    University of British Columbia, Vancouver, British Columbia V6Z1Y6, Canada
    J Biol Chem 283:12898-908. 2008
    ..These data identify a novel mechanism for TGFbeta1 signaling in human lung fibroblasts by which they may contribute to normal and pathological wound healing...
  28. ncbi Specification of the direction of adhesive signaling by the integrin beta cytoplasmic domain
    Elena G Arias-Salgado
    Department of Medicine, University of California San Diego, La Jolla, 92093 0726, USA
    J Biol Chem 280:29699-707. 2005
    ..in c-Src binding and found that amino acid exchanges affecting a combination of an Arg and Thr residue in the integrin beta3 tail control the binding specificity for SFKs but have no effect on talin binding...
  29. pmc Rationally designed integrin beta3 mutants stabilized in the high affinity conformation
    Bing Hao Luo
    Immune Disease Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 284:3917-24. 2009
    ..This detailed understanding of the conformational change will assist in the development of allosteric drugs that either stabilize or destabilize specific integrin conformations without occluding the ligand-binding site...
  30. ncbi Calpain cleavage of the cytoplasmic domain of the integrin beta 3 subunit
    X Du
    Department of Vascular Biology, Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 270:26146-51. 1995
    ..Thus, calpain cleavage of the beta 3 cytoplasmic domain may provide a means to regulate integrin signaling functions...
  31. ncbi Signal co-operation between integrins and other receptor systems
    Charles H Streuli
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, U K
    Biochem J 418:491-506. 2009
    ....
  32. pmc Multiscale simulations suggest a mechanism for integrin inside-out activation
    Antreas C Kalli
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 108:11890-5. 2011
    ..These results, combined with various published experimental observations, suggest a model for the mechanism of inside-out activation of integrins by talin...
  33. pmc NF-kappaB mediates alphavbeta3 integrin-induced endothelial cell survival
    M Scatena
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Cell Biol 141:1083-93. 1998
    ..In contrast, inhibition of MEK and PI3-kinase did not affect osteopontin-induced NF-kappaB activation. These studies identify NF-kappaB as an important signaling molecule in alphavbeta3 integrin-mediated endothelial cell survival...
  34. ncbi Integrin beta3 down-regulates invasive features of ovarian cancer cells in SKOV3 cell subclones
    Jie Chen
    Department of Maternal and Child Health Care, School of Public Health, Shandong University, 250012, Jinan, China
    J Cancer Res Clin Oncol 135:909-17. 2009
    To investigate the role of integrin beta3 in invasive features of ovarian cancer SKOV3 cells, by comparing different metastatic subclones.
  35. ncbi Dynamics of beta3 integrin I-like and hybrid domains: insight from simulations on the mechanism of transition between open and closed forms
    Thomas Gaillard
    Laboratoire de Biophysicochimie Moléculaire, Institut de Chimie de Strasbourg, Universite de Strasbourg, Strasbourg, France
    Proteins 76:977-94. 2009
    ..From our results, we propose that these two helices function in tandem to initiate large-scale, interdomain conformational transition apparent in integrin activation and signaling...
  36. pmc Targeted molecular dynamics reveals overall common conformational changes upon hybrid domain swing-out in beta3 integrins
    Davide Provasi
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Proteins 77:477-89. 2009
    ..Taken together, our results suggest novel testable hypotheses of intradomain and interdomain interactions responsible for beta3 integrin activation...
  37. ncbi Complete loss of PTEN expression as a possible early prognostic marker for prostate cancer metastasis
    Martine Schmitz
    Laboratoire de Biologie et Physiologie Intégrée LBPI, Université du Luxembourg, 162A Avenue de la Faïencerie, L 1511 Luxembourg
    Int J Cancer 120:1284-92. 2007
    ..These findings demonstrate that loss of PTEN expression is an important factor in progression towards metastatic disease and could potentially serve as an early prognostic marker for prostate cancer metastasis...
  38. ncbi Beta3 integrin deficiency promotes cardiac hypertrophy and inflammation
    Jie Ren
    Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mol Cell Cardiol 42:367-77. 2007
    ..These results suggest that alpha(v)beta(3) expression in bone marrow has a generalized suppressive effect on cardiac inflammation...
  39. pmc A push-pull mechanism for regulating integrin function
    Wei Li
    Department of Medicine, Hematology Oncology Division, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:1424-9. 2005
    ....
  40. ncbi A direct test of potential roles for beta3 and beta5 integrins in growth and metastasis of murine mammary carcinomas
    Daniela Taverna
    Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cancer Res 65:10324-9. 2005
    ..These data indicate that alphavbeta3 or alphavbeta5 integrins are not essential for tumor growth and progression, although they might play some role in mammary gland development...
  41. ncbi Beta(3) integrin expression increases breast carcinoma cell responsiveness to the malignancy-enhancing effects of osteopontin
    Kara A Furger
    Department of Oncology, University of Western Ontario, Ontario, Canada
    Mol Cancer Res 1:810-9. 2003
    ....
  42. pmc Molecular insight into human platelet antigens: structural and evolutionary conservation analyses offer new perspective to immunogenic disorders
    Meytal Landau
    Amalia Biron Research Institute of Thrombosis and Hemostasis, Chaim Sheba Medical Center, Tel Hashomer, Israel
    Transfusion 51:558-69. 2011
    ....
  43. ncbi Missense mutations in the beta(3) subunit have a different impact on the expression and function between alpha(IIb)beta(3) and alpha(v)beta(3)
    Seiji Tadokoro
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University, 2 2 Yamadaoka, Suita Osaka 565 0871, Japan
    Blood 99:931-8. 2002
    ..Present data would be a clue to elucidate the regulatory mechanism of expression and function of beta(3) integrins...
  44. ncbi Mutations in GPIIIa molecule as a cause for Glanzmann thrombasthenia in Indian patients
    S Nair
    Institute of Immunohaematology ICMR, 13th Floor, New Building, KEM Hospital, Parel, Mumbai 400012, India
    J Thromb Haemost 3:482-8. 2005
    ..In this report we describe the molecular studies on 22 patients with Glanzmann Thrombasthenia at our institute...
  45. pmc Integrin beta3 regions controlling binding of murine mAb 7E3: implications for the mechanism of integrin alphaIIbbeta3 activation
    Andrea Artoni
    Laboratory of Blood and Vascular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:13114-20. 2004
    ..In this study we identified regions on integrin beta3 that control 7E3 binding...
  46. ncbi A functional comparison of mutations in integrin beta cytoplasmic domains: effects on the regulation of tyrosine phosphorylation, cell spreading, cell attachment and beta1 integrin conformation
    A L Bodeau
    The Center for Cell Biology and Cancer Research, Albany Medical College, NY 12208, USA
    J Cell Sci 114:2795-807. 2001
    ....
  47. ncbi Two different beta3 cysteine substitutions alter alphaIIb beta3 maturation and result in Glanzmann thrombasthenia
    S Milet-Marsal
    UMR 5533 CNRS, Hopital Cardiologique, Pessac, France
    Thromb Haemost 88:104-10. 2002
    ..We then showed by metabolic (35S) labeling and Endo-H glycosidase treatment that these substitutions strongly affected complex maturation within the cell...
  48. ncbi In vivo characterization of the integrin beta3 as a receptor for Hantaan virus cellular entry
    Jin Won Song
    Department of Microbiology and the Institute for Viral Diseases, Division of Brain Korea 21 Program for Biomedical Science, College of Medicine Korea University, Seoul 136 705, Korea
    Exp Mol Med 37:121-7. 2005
    ..Our data demonstrate that the beta(3) receptor is important for HTNV infection in vivo, but also suggest that HTNV may utilize additional receptors beyond beta(3) for cellular entry within an organism...
  49. ncbi Two new beta3 integrin mutations in Indian patients with Glanzmann thrombasthenia: localization of mutations affecting cysteine residues in integrin beta3
    Sona Nair
    Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Thromb Haemost 88:503-9. 2002
    ..Patient 2 had an insertion mutation resulting in a frameshift and premature termination. Both mutations affect biogenesis of platelet alphaIIbbeta3 receptors...
  50. ncbi Serine 752 in the cytoplasmic domain of the beta 3 integrin subunit is not required for alpha v beta 3 postreceptor signaling events
    N Kieffer
    Laboratoire Franco Luxembourgeois de Recherche Biomédicale, Center Universitaire Luxembourg, Grand Duchy
    Cell Adhes Commun 4:25-39. 1996
    ....
  51. ncbi Three novel integrin beta3 subunit missense mutations (H280P, C560F, and G579S) in thrombasthenia, including one (H280P) prevalent in Japanese patients
    H Ambo
    School of Medicine, Keio University, Tokyo, 160 8582, Japan
    Biochem Biophys Res Commun 251:763-8. 1998
    ..direct sequencing of platelet RNA and genomic DNA revealed three single nucleotide substitutions of the integrin beta3 subunit gene (His (CAT)-280 to Pro (CCT), Cys (TGT)-560 to Phe (TTT), and Gly(GGC)-579 to Ser(AGC))...
  52. pmc Soluble CD40 ligand induces beta3 integrin tyrosine phosphorylation and triggers platelet activation by outside-in signaling
    K S Srinivasa Prasad
    Department of Cardiovascular Biology, Millennium Pharmaceuticals, Inc, 256 East Grand Avenue, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 100:12367-71. 2003
    ..These data indicate that sCD40L is a primary platelet agonist and that platelet stimulation is induced by the binding of the KGD domain of sCD40L to alphaIIbbeta3, triggering outside-in signaling by tyrosine phosphorylation of beta3...
  53. ncbi Differential involvement of beta3 integrin in pre- and postsynaptic forms of adaptation to chronic activity deprivation
    Lorenzo A Cingolani
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, UCL, Gower Street, WC1E 6BT London, UK
    Neuron Glia Biol 4:179-87. 2008
    ..Our findings define the differential involvement of beta3 integrin in two forms of synaptic homeostasis...
  54. ncbi Blockade of integrin beta3-FAK signaling pathway activated by osteopontin inhibits neointimal formation after balloon injury
    Mei Han
    Department of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, China
    Cardiovasc Pathol 16:283-90. 2007
    ..promotes the migration and adhesion of vascular smooth muscle cells (VSMCs) through cell surface receptor, integrin beta3. In order to elucidate the signaling pathway by which OPN is involved in neointimal formation, we focused on ..
  55. ncbi Toward a prophylaxis against fetal and neonatal alloimmune thrombocytopenia: induction of antibody-mediated immune suppression and prevention of severe clinical complications in a murine model
    Heidi Tiller
    University Hospital of North Norway, Tromsø, Norway
    Transfusion 52:1446-57. 2012
    ..This preclinical proof-of-concept study investigated whether passive administration of anti-β3 integrin could induce AMIS and thereby prevent clinical complications of FNAIT...
  56. ncbi Integrin beta3 Leu33Pro homozygosity and risk of cancer
    Stig E Bojesen
    Department of Clinical Biochemistry, Herlev University Hospital, Herlev, Denmark
    J Natl Cancer Inst 95:1150-7. 2003
    ..The Leu33Pro polymorphism of the beta3 subunit modulates the function of alpha(IIb)beta3 integrin. We examined whether this polymorphism influences cancer risk...
  57. ncbi Over-expression of integrin beta3 can partially overcome the defect of integrin beta3 signaling in transglutaminase 2 null macrophages
    Beáta Tóth
    Department of Biochemistry and Molecular Biology, Apoptosis and Genomics Research Group, Hungarian Academy of Sciences, University of Debrecen, Nagyerdei krt 98, Debrecen H 4012, Hungary
    Immunol Lett 126:22-8. 2009
    ..Our data provide a proof for the concept that the function of TG2 is to stabilize accumulated integrin beta(3) concentration in the phagocytic cup...
  58. ncbi Shear stress augments the enhanced adhesive phenotype of cells expressing the Pro33 isoform of integrin beta3
    K Vinod Vijayan
    Department of Medicine, Baylor College of Medicine, One Baylor Plaza, BCM 286, N1319, Houston, TX 77030, USA
    FEBS Lett 540:41-6. 2003
    ..Thus, shear stress enhances the adhesive phenotype of the Pro33 cells to multiple physiologic substrates...
  59. ncbi A Ser162-->Leu mutation within glycoprotein (GP) IIIa (integrin beta3) results in an unstable alphaIIbbeta3 complex that retains partial function in a novel form of type II Glanzmann thrombasthenia
    D E Jackson
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee 53233 2194, USA
    Thromb Haemost 80:42-8. 1998
    ..Taken together, these data implicate the region encompassing Ser162 in the stabilization and ligand binding properties of the alphaIIbbeta3 complex...
  60. ncbi Major mutations in calf-1 and calf-2 domains of glycoprotein IIb in patients with Glanzmann thrombasthenia enable GPIIb/IIIa complex formation, but impair its transport from the endoplasmic reticulum to the Golgi apparatus
    Nurit Rosenberg
    Thrombosis and Hemostasis Research Institute, The Chaim Sheba Medical Center, Tel Hashomer, Israel
    Blood 101:4808-15. 2003
    ..These data suggest that despite partial disruption of calf-1 or calf-2 domain, GPIIb/IIIa complex is formed but its transport from the endoplasmic reticulum is impaired...
  61. ncbi RGD-containing peptides activate S6K1 through beta3 integrin in adult cardiac muscle cells
    Sundaravadivel Balasubramanian
    Cardiology Division of the Department of Medicine, Gazes Cardiac Research Institute, Medical University of South Carolina, 114 Doughty Street, Charleston, SC 29425 2221, USA
    J Biol Chem 278:42214-24. 2003
    ....
  62. ncbi Effects of ovarian stimulation on endometrial integrin beta3 and leukemia inhibitory factor expression in the peri-implantation phase
    Qiu ju Chen
    Department of Reproductive Medicine, International Peace Maternity and Child Health Hospital, Shanghai, Peoples Republic of China
    Fertil Steril 89:1357-63. 2008
    ..To investigate the effects of ovarian stimulation on endometrial receptivity in the peri-implantation phase...
  63. ncbi Analysis of plasminogen activator inhibitor-1, integrin beta3, beta fibrinogen, and methylenetetrahydrofolate reductase polymorphisms in Iranian women with recurrent pregnancy loss
    Mahmood Jeddi-Tehrani
    Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Shahid Beheshti University, Evin, Tehran, Iran
    Am J Reprod Immunol 66:149-56. 2011
    ....
  64. ncbi Glanzmann's thrombasthenia: identification of 19 new mutations in 30 patients
    Giovanna D'Andrea
    Unita di Aterosclerosi e Trombosi, I R C C S Casa Sollievo della Sofferenza, S Giovanni Rotondo, Italy
    Thromb Haemost 87:1034-42. 2002
    ..In conclusion, we have identified a spectrum of unreported mutations that may be of value to unravel the role of specific regions of alphaIIb and beta3 genes...
  65. ncbi β(1) and β(3) integrins disassemble from basal focal adhesions and β(3) integrin is later localised to the apical plasma membrane of rat uterine luminal epithelial cells at the time of implantation
    Yui Kaneko
    School of Medical Sciences Discipline of Anatomy and Histology and The Bosch Institute, Anderson Stuart Building, The University of Sydney, Sydney, NSW 2006, Australia
    Reprod Fertil Dev 23:481-95. 2011
    ..Taken together, β(1) and β(3) integrin disassembly from focal adhesions and the increase in β(3) integrin apically are key components of hormonally regulated endometrial receptivity...
  66. pmc Absence of beta3 integrin accelerates early skeletal repair
    Diane Hu
    Orthopaedic Trauma Institute, San Francisco General Hospital, University of California at San Francisco, 1001 Potrero Avenue, San Francisco, California 94110, USA
    J Orthop Res 28:32-7. 2010
    ..In conclusion, our findings demonstrate that beta3 integrin plays an important role during early fracture healing. Further research is required to determine the underlying mechanisms...
  67. pmc Lack of beta3 integrin signaling contributes to calpain-mediated myocardial cell loss in pressure-overloaded myocardium
    Geetha Suryakumar
    Cardiology Division of the Department of Medicine, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston, South Carolina 29425 2221, USA
    J Cardiovasc Pharmacol 55:567-73. 2010
    ..Because beta3 integrin contributes to cardioprotective signaling, these studies indicate that the loss of specific integrin function could be a key mechanism for calpain-mediated programmed cell death of cardiomyocytes in PO myocardium...
  68. ncbi Effects of hydrosalpinx on pinopodes, leukaemia inhibitory factor, integrin beta3 and MUC1 expression in the peri-implantation endometrium
    Lu Li
    Reproductive Medicine Center of The International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai, PR China
    Eur J Obstet Gynecol Reprod Biol 151:171-5. 2010
    ..To compare the expression of pinopodes, LIF, integrin beta(3) and MUC1 in the peri-implantation endometrium of women with and without hydrosalpinx...
  69. ncbi Beta3 tyrosine phosphorylation in alphaIIbbeta3 (platelet membrane GP IIb-IIIa) outside-in integrin signaling
    D R Phillips
    COR Therapeutics, Inc, South San Francisco, CA 94080, USA
    Thromb Haemost 86:246-58. 2001
    ..Taken together, the data suggest that the beta3 tyrosine phosphorylation signaling mechanism is important to alphaIIbbeta3 function and might be applicable to a wide variety of integrin-mediated events...
  70. pmc The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells
    Weiyi Feng
    Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Cell Biol 183:1145-57. 2008
    ..Thus, beta(3) integrin has the potential to control processes such as tumor growth and wound healing by regulating BMDC recruitment to sites undergoing pathological and adaptive angiogenesis...
  71. pmc Integrin {beta}3 phosphorylation dictates its complex with the Shc phosphotyrosine-binding (PTB) domain
    Lalit Deshmukh
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, Connecticut 06269 3092, USA
    J Biol Chem 285:34875-84. 2010
    ..Detailed comparison with the known Shc PTB structure bound to a target TrkA peptide revealed some significant differences, which shed new light upon the PTB domain specificity...
  72. ncbi Disruption of the beta3 663-687 disulfide bridge confers constitutive activity to beta3 integrins
    Nora Butta
    Department of Pathophysiology and Human Molecular Genetics, Centro de Investigaciones Biologicas, Madrid, Spain
    Blood 102:2491-7. 2003
    ..However, a basal, nonactivated, low ligand-affinity state of the beta3 integrins demands a normal conformation of this domain...
  73. ncbi Probing conformational changes in the I-like domain and the cysteine-rich repeat of human beta 3 integrins following disulfide bond disruption by cysteine mutations: identification of cysteine 598 involved in alphaIIbbeta3 activation
    P Chen
    , Centre Universitaire, L-1511 Luxembourg
    J Biol Chem 276:38628-35. 2001
    ....
  74. ncbi Phosphorylation of beta3 integrin controls ligand binding strength
    Anirban Datta
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6076, USA
    J Biol Chem 277:3943-9. 2002
    ..Thus, phosphorylation of the cytoplasmic domain of beta(3) is a negative regulator of alpha(v)beta(3)-fibronectin binding strength...
  75. pmc Gonadotropin-releasing hormone (GnRH)-I and GnRH-II induce cell growth inhibition in human endometrial cancer cells: involvement of integrin beta3 and focal adhesion kinase
    Dong Wook Park
    Department of Obstetrics and Gynecology, Child and Family Research Institute, The University of British Columbia, Vancouver, British Columbia, V6H 3V5, Canada
    Reprod Biol Endocrinol 7:81. 2009
    ..Thus, in the present study, we further examined the possibility that GnRH induces integrin beta3 and activation of focal adhesion kinase (FAK) through mitogen-activated protein kinases (MAPKs), ERK1/2 and ..
  76. ncbi Characterization of the expression of key adenoviral receptors CAR and integrin beta3/beta5 subunits on the membrane of human NT2 neurons
    Deqi Huang
    Experimental Stroke Group, NRC Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada
    J Mol Neurosci 24:323-8. 2004
    ..These observations suggest that modulating the expression of integrin subunits beta3/5 or the functional heterodimer alphavbeta3/5 in human NT2/Ns may enhance adenoviral infectivity of NT2/Ns...
  77. pmc Role of molecular mimicry of hepatitis C virus protein with platelet GPIIIa in hepatitis C-related immunologic thrombocytopenia
    Wei Zhang
    Department of Medicine, New York University School of Medicine, NY 10016, USA
    Blood 113:4086-93. 2009
    ..001). Thus, HCV core envelope 1 can induce thrombocytopenia by molecular mimicry with GPIIIa49-66...
  78. ncbi Platelet fragmentation requires a specific structural conformation of human monoclonal antibody against beta3 integrin
    Zongdong Li
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 283:3224-30. 2008
    ..Thus, a structural change in the conformation of anti-GPIIIa49-66 antibody contributes to its binding to the beta3 integrin and subsequent antibody-induced platelet fragmentation and aggregate dissolution...
  79. ncbi Three novel mutations in the glycoprotein IIb gene in a patient with type II Glanzmann thrombasthenia
    Gergely Losonczy
    Clinical Research Center, Thrombosis and Hemostasis Research Group of the Hungarian Academy of Sciences, University of Debrecen, Debrecen, Hungary
    Haematologica 92:698-701. 2007
    ..The p.His782Asn mutation compromised transport of the pro-GPIIb/IIIa complex from the endoplasmic reticulum to the Golgi, hindering its maturation and surface expression...
  80. ncbi Discrete functional motifs reside within the cytoplasmic tail of alphaV integrin subunit
    Thomas A Haas
    Department of Anatomy and Cell Biology and Molecular Design Research Group, College of Medicine, University of Saskatchewan, Saskatoon, Canada
    Thromb Haemost 99:96-107. 2008
    ....
  81. ncbi HPA-1a phenotype-genotype discrepancy reveals a naturally occurring Arg93Gln substitution in the platelet beta 3 integrin that disrupts the HPA-1a epitope
    Nicholas A Watkins
    Department of Haematology, Division of Transfusion Medicine, University of Cambridge, United Kingdom
    Blood 99:1833-9. 2002
    ..Our results show that an Arg93Gln mutation in the beta 3Leu33 encoding allele disrupts the HPA-1a epitope, suggesting that Arg93 contributes to the formation of the HPA-1a B-cell epitope...
  82. ncbi A naturally occurring LeuVal mutation in beta3-integrin impairs the HPA-1a epitope: the third allele of HPA-1
    Sentot Santoso
    Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany
    Transfusion 46:790-9. 2006
    ..Alloimmunization against HPA-1a (beta3-Leu33) is the most frequent cause of neonatal alloimmune thrombocytopenia and posttransfusion purpura...
  83. pmc Latent KSHV infection of endothelial cells induces integrin beta3 to activate angiogenic phenotypes
    Terri A Dimaio
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 7:e1002424. 2011
    ..KSHV induction of integrin β3, leading to induction of angiogenic and cancer cell phenotypes during latency, is likely to be important for KS tumor formation and potentially provides a novel target for treating KS tumors...
  84. pmc Therapeutic targeting of the focal adhesion complex prevents oncogenic TGF-beta signaling and metastasis
    Michael K Wendt
    Department of Pharmacology, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado 80045, USA
    Breast Cancer Res 11:R68. 2009
    ..However, the molecular mechanisms by which the focal adhesion complex contributes to beta3 integrin:TbetaR-II signaling and the oncogenic conversion of TGF-beta remain poorly understood...
  85. ncbi An integrin phosphorylation switch: the effect of beta3 integrin tail phosphorylation on Dok1 and talin binding
    Camilla L Oxley
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 283:5420-6. 2008
    ..This switch may represent an in vivo mechanism for control of integrin receptor activation. These results have implications for the control of integrin signaling by proteins containing phosphotyrosine binding domains...
  86. ncbi Four potential biomarkers as prognostic factors in stage III serous ovarian adenocarcinomas
    Karolina Partheen
    Department of Oncology, University of Gothenburg, Gothenburg, Sweden
    Int J Cancer 123:2130-7. 2008
    ..According to our results, the 4 biomarkers CLU, ITGB3, PRAME and CAPG may be used as prognostic factors for patients with stage III serous ovarian adenocarcinomas...
  87. ncbi Adenoviral-encoded antigens are presented efficiently by a subset of dendritic cells expressing high levels of alpha(v)beta3 integrins
    Airi Harui
    Division of Pulmonary and Critical Care Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1690, USA
    J Leukoc Biol 79:1271-8. 2006
    ..These results suggest that a limited subset of DC expressing high levels of alpha(v)beta3 integrins is preferentially transduced by Ad vectors and activates CD8+ T cell responses against Ad-encoded antigens...
  88. pmc Mice lacking beta3 integrins are osteosclerotic because of dysfunctional osteoclasts
    K P McHugh
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 105:433-40. 2000
    ..Thus, although it is not required for osteoclastogenesis, the integrin alphavbeta3 is essential for normal osteoclast function...
  89. pmc New low-frequency platelet glycoprotein polymorphisms associated with neonatal alloimmune thrombocytopenia
    Julie A Peterson
    Blood Research Institute and Platelet and Neutrophil Immunology Laboratory, BloodCenter of Wisconsin, Milwaukee, Wisconsin 53201 2178, USA
    Transfusion 50:324-33. 2010
    ..Recent reports suggest that maternal immunization against low-frequency, platelet (PLT)-specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought...
  90. ncbi The roles of THY1 and integrin beta3 in cell adhesion during theca cell layer formation and the effect of follicle-stimulating hormone on THY1 and integrin beta3 localization in mouse ovarian follicles
    Saori Itami
    School of Natural Science and Ecological Awareness, Graduate School of Humanities and Sciences, Faculty of Science, Nara Women s University, Nara, Japan
    Biol Reprod 84:986-95. 2011
    ..In the present study, we examined the roles of THY1 and integrin beta3 in theca cell layer formation during mouse folliculogenesis...
  91. ncbi Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain
    E Borges
    Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 275:39867-73. 2000
    ..These results show that the interactions that lead to the association of the alpha(v)beta(3) integrin with PDGF-Rbeta and VEGF-R2 and enhancement of RTK activity take place outside the cell...
  92. ncbi Integrin beta3 overexpression suppresses tumor growth in a human model of gliomagenesis: implications for the role of beta3 overexpression in glioblastoma multiforme
    Masayuki Kanamori
    Department of Neurological Surgery and The Brain Tumor Research Center, University of California San Francisco, San Francisco, California, USA
    Cancer Res 64:2751-8. 2004
    ..These results show that tumor-specific alphaVbeta3 overexpression has growth-suppressive effects in gliomas, but that these deleterious effects are mitigated by alterations common to alphaVbeta3-overexpressing GBM...
  93. ncbi A functional platelet fibrinogen receptor with a deletion in the cysteine-rich repeat region of the beta(3) integrin: the Oe(a) alloantigen in neonatal alloimmune thrombocytopenia
    Sentot Santoso
    Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University Giessen, Germany
    Blood 99:1205-14. 2002
    ..It can be assumed that the Oe(a) form did arise as a result of a mutational event from an already mutated GPIIIa allele...
  94. ncbi Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins
    Louise E Reynolds
    Cell Adhesion and Disease Laboratory, Richard Dimbleby Department, Imperial Cancer Research Fund, St Thomas Hospital, London, UK
    Nat Med 8:27-34. 2002
    ....
  95. ncbi Phosphoinositide 3-kinase modulation of beta(3)-integrin represents an endogenous "braking" mechanism during neutrophil transmatrix migration
    W J Bruyninckx
    Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Blood 97:3251-8. 2001
    ..These data indicate that PI3K-regulated beta(3)-integrin represents a natural "braking" mechanism for PMNs during transit through the extracellular matrix...
  96. ncbi Three novel beta3 domain-deletion peptides for the sensitive and specific detection of HPA-4 and six low frequency beta3-HPA antibodies
    P Stafford
    Department of Haematology, University of Cambridge and National Health Service Blood and Transplant, Cambridge, UK
    J Thromb Haemost 6:376-83. 2008
    ..Recombinant allelic beta3 peptides, expressed at high levels would improve consistency in antibody detection, but the expression of soluble and monomeric integrins expressing complex dependent epitopes has previously proved challenging...
  97. pmc VEGF-integrin interplay controls tumor growth and vascularization
    Sarmishtha De
    Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Proc Natl Acad Sci U S A 102:7589-94. 2005
    ..These observations identify a link between beta3 integrins and VEGF in tumor growth and angiogenesis and, therefore, may influence anti-integrin as well as anti-VEGF therapeutic strategies...
  98. ncbi The specificity and function of the metal-binding sites in the integrin beta3 A-domain
    Michelle M Pesho
    Joseph J Jacobs Center for Thrombosis and Vascular Biology and Department of Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 281:23034-41. 2006
    ..These results indicate that the specificities of the three metal-binding sites for divalent cations are distinct and that each site can regulate the ligand binding potential of the beta3 A-domain...
  99. ncbi Beta 3 integrin phosphorylation is essential for Arp3 organization into leukocyte alpha V beta 3-vitronectin adhesion contacts
    Surjit K Chandhoke
    Department of Cell and Developmental Biology SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    J Cell Sci 117:1431-41. 2004
    ....
  100. ncbi Antitumor activity of bacteriophages in murine experimental cancer models caused possibly by inhibition of beta3 integrin signaling pathway
    K Dabrowska
    Institute of Immunology and Experimental Therapy, 53 114 Wroclaw, Poland
    Acta Virol 48:241-8. 2004
    ..This is in line with the well-described beta3 integrin-dependent mechanism of tumor metastasis. It is concluded that the blocking of beta3 integrins by phage preparations results in a significant decrease in tumor invasiveness...
  101. ncbi Elevated Flk1 (vascular endothelial growth factor receptor 2) signaling mediates enhanced angiogenesis in beta3-integrin-deficient mice
    Andrew R Reynolds
    Tumour Biology Laboratory, Cancer Research UK Clinical Centre, Barts and The London, Queen Mary s School of Medicine and Dentistry, John Vane Science Centre, London, United Kingdom
    Cancer Res 64:8643-50. 2004
    ..These data confirm that VEGF signaling via Flk1 is enhanced in beta(3)-integrin-deficient mice and suggests that this increase may mediate the enhanced angiogenesis and tumor growth observed in these mice in vivo...

Research Grants63

  1. HTS platform for identification of regulators of cell adhesion molecule signaling
    Darren G Woodside; Fiscal Year: 2013
    ..In our first aim, we propose to develop a suite of primary HTS assays targeting the binding of integrin beta3 cytoplasmic domains with Syk, Kindlin-3, and Galpha13...
  2. INTEGRIN SIGNALING IN HEMOSTASIS AND BLOOD DISEASES
    Sanford J Shattil; Fiscal Year: 2012
    ..The synergy achieved by this Program will further our understanding of integrin signaling, with implications for disorders of cell adhesion affecting hemostasis and vascular repair. ..
  3. Cyr61-induced breast cancer: Clinical relevance and therapeutic development
    Ruth Lupu; Fiscal Year: 2010
    ..e., Cyr61/alpha-v-beta3). In addition, we will evaluate the relevance of a Cyr61- triggered "Cyr61/alpha-v-beta3 integrin loop" as a novel predictive marker for response to chemotherapy in breast cancer. ..
  4. Integrin Signaling In Vascular Cells
    Susan Smyth; Fiscal Year: 2009
    ..abstract_text> ..
  5. Structure and function of beta3 integrins on blood cells
    EDWARD FRANKLIN PLOW; Fiscal Year: 2013
    ..abstract_text> ..
  6. Molecular Mechanisms of Cocaine-Induced Alterations in Accumbens AMPA Receptors
    Rachel J Smith; Fiscal Year: 2012
    ..Determining the processes involved in the development of addiction may lead to novel therapeutic options aimed at reversing drug-induced alterations. ..
  7. BIOSYNTHESIS NAD STRUCTURE OF THE FIBRINOGEN RECEPTOR
    Paul Bray; Fiscal Year: 1990
    ..By genetically engineering deletions in the cDNA, we hope to localize the binding site on one and/or both glycoproteins...
  8. CELLULAR CONSEQUENCES OF DEFECTIVE IIB AND IIIA GENES
    Paul Bray; Fiscal Year: 1993
    ..Finally, apparent defects in transcriptional controlling elements will be studied both in CAT assays and in assays that detect alterations in mRNA level and stability...
  9. A platelet ligand mimetic for neurothrombotic disorders
    Catherine Woods; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  10. HANTAVIRUS CELL INTERACTIONS
    ERICH MACKOW; Fiscal Year: 2003
    ..Specific Aims: 1) beta3 Integrin Requirements for Hantavirus Infectivity; 2) Blocking beta3 Integrin-Hantavirus Interactions; 3) Transgenic Model of Hantavirus Infection and Pathogenesis. ..
  11. Genetic Modifiers in Children with Sickle Cell Anemia
    Russell Ware; Fiscal Year: 2005
    ..abstract_text> ..
  12. BIOSYNTHESIS AND GENETICS OF GLYCOPROTEINS IIB AND IIIA
    Marc Shuman; Fiscal Year: 1993
    ..Promoter and enhancer elements of both genes will be identified using chloramphenicol acetyltransferase (CAT) assays. CAT constructs will be transfected into HEL cells and assayed following stimulation of GPIIb-IIIa synthesis by DMSO...
  13. HIV Associated Thrombocytopenia
    Zongdong Li; Fiscal Year: 2009
    ..It will also provide a new insight into the mechanism of this novel and unique discovery of antibody-induced fragmentation of platelets via direct activation of platelet NADP Oxidase ..
  14. STRUCTURAL STUDIES OF PLATELET GPIIB/IIIA ACTIVATION
    JOEL BENNETT; Fiscal Year: 1999
    ..Moreover, this information will provide a paradigm for a general understanding of the relationship of integrin structure and function. ..
  15. Regulation of GPIIb-IIIa by the platelet cytoskeleton
    Joan Fox; Fiscal Year: 2003
    ..Further, the studies may lead to an increased understanding of key mechanisms involved in integrin-mediated functions such as platelet adhesion and aggregation, wound healing, angiogenesis, inflammation, metastasis, and cell migration. ..
  16. ADVENTITIAL RESPONSE TO VASCULAR INJURY: ESTROGEN
    Suzanne Oparil; Fiscal Year: 2003
    ..These fundamental mechanistic studies will establish more rational strategies for therapeutic intervention in vascular diseases, including the basis for future gene therapy. ..
  17. Dendritic Diblock Copolymer Micelles as New Targeted Drug Delivery Systems
    Paula Hammond; Fiscal Year: 2006
    ..abstract_text> ..
  18. BETA3 INTEGRIN PROMOTER ACTIVATION IN OSTEOCLASTOGENESIS
    Kevin McHugh; Fiscal Year: 2004
    The integrin beta3 gene is highly expressed in osteoclasts and during osteoclastogenesis yet the beta3 promoter is nearly silent in bone marrow macrophage, the osteoclast precursor...
  19. GLYCOPROTEINS IIB AND IIIA IN PLATELETS AND NEUTROPHILS
    Lisa Jennings; Fiscal Year: 1991
    ..This study should provide information on the relationship of platelet antigens to the function of normal and abnormal leukocytes...
  20. INTERACTION OF CELLS WITH POLYMERIZING FIBRIN
    Stefan Niewiarowski; Fiscal Year: 1992
    ..We propose to test the hypothesis that polymerizing fibrin monomers bind to platelet surface receptors other than those associated with the GPIlb/IIIa complex...
  21. THE IMMUNOGENICITY OF HUMAN PLATELET PROTEINS
    Thomas Kunicki; Fiscal Year: 1990
    ....
  22. MODELING OF PLATELET ALLOANTIGENS
    Emily Barron Casella; Fiscal Year: 2002
    ..The ability of HPA-1a murine GPIIIa to assemble into a fibrinogen receptor and bind ligand in transfected megakaryoblastic cells will also be tested. ..
  23. MICROVASCULAR FAILURE IN MURINE AND PRIMATE STROKE
    E Connolly; Fiscal Year: 2000
    ....
  24. Molecular Analysis fo Borrelia P66-Integrin Binding
    Jenifer Coburn; Fiscal Year: 2009
    ..burgdorferi. Through this work, we expect to gain insight into how pathogenic spirochetes manipulate the biology of their mammalian hosts, and into how integrins recognize unconventional ligands. ..
  25. PLATELET P1A2--STUDIES ON A PRO THROMBOTIC POLYMORPHISM
    Paul Bray; Fiscal Year: 2001
    ..The major goal of this proposal is to characterize functional differences between the PlA1 and PlA2 forms of integrin beta3 as it exists in the two important receptors, alphaIIb-beta3 and alpha-v- beta3...
  26. PU1 TRANSCRIPTION FACTOR AND OSTEOCLASTOGENESIS
    Mehrdad Tondravi; Fiscal Year: 2000
    ..1 mutation is inherent to the osteoclast (myeloid) precursor; 2) PU.1 expression is required throughout the temporal spectrum of osteoclast differentiation; and 3) PU.1 regulates expression of the osteoclast beta3 integrin gene. ..
  27. Spectral Imaging of Melanoma Cell Adhesion & Metastasis
    Dorothea Becker; Fiscal Year: 2007
    ....
  28. Regulation of Osteoclast Activity by Calcium and cGMP
    Harry C Blair; Fiscal Year: 2010
    ..The mechanisms defined by these studies will highlight potential targets for pharmacological intervention in osteoporosis. ..
  29. CALRETICULIN AND OSTEOCLAST FUNCTION
    Xu Cao; Fiscal Year: 2001
    ..2D3), which prompts osteoclast differentiation in vivo and in vitro, transcriptionally activate expression of integrin beta3 gene promoter region...
  30. MOLECULAR BIOLOGY OF CYTOADHESINS--ORAL TISSUE MODEL
    GILBERT WHITE; Fiscal Year: 1990
    ..Finally, we will initiate studies to express GPIIb and GPIIIa in eukaryotic cells with a long range goal of starting in vitro mutagenesis studies to further probe the structure-function relationships of these two proteins...
  31. CELL ADHESION MECHANISMS IN VASCULAR DISEASE
    Mark Ginsberg; Fiscal Year: 2003
    ..knockout mice to generate mice with reduced platelet alpha2beta1 and will maintain stocks of vWF, TSP-2, integrin beta3, and integrin beta5 deficient mice. An administrative will continue to provide administrative support...
  32. Collagen-Binding Integrins in Intimal Hyperplasia
    Eric Choi; Fiscal Year: 2006
    ..Next, we plan to understand the role of collagenases in the interplay of SMC migration on native collagen. Finally, we will block the critical pathways of SMC migration in vivo to eliminate intimal lesion formation. ..
  33. PEPTIDE INHIBITORS OF PLATELET AGGREGATION
    Israel Charo; Fiscal Year: 1990
    ..The therapeutics developed from this approach will be used in a wide-range of clinical situations characterized by thrombosis, such as unstable angina, stroke, and reocclusion after angioplasty...
  34. ANTIBODIES IN PATHOGENESIS OF HIV-1 TROMBOCYTOPENIA
    Henrik Ditzel; Fiscal Year: 2003
    ..He will also look for molecular mimicry with foreign Ag's. ..
  35. RADIOLABELED ANTIVASCULAR TARGETING AGENTS
    Marlene Hauck; Fiscal Year: 2001
    ..Dual-label techniques using RGD and a DRG peptides labeled with I125 and I131 respectively will be used to determine normal tissue and distribution and tumor uptake. ..
  36. ATHEROSCLEROSIS, DIABETES AND LPL
    CLAY SEMENKOVICH; Fiscal Year: 2005
    ..These experiments could lead to innovative strategies for the treatment of vascular disease in diabetes. ..
  37. Activation of Sickle Red Cell Adhesion
    Rahima Zennadi; Fiscal Year: 2006
    ..Overall, these experiments will elucidate the molecular mechanisms of LW-mediated SS RBC adhesion to endothelium and leukocytes, and thus how physiologic stress and stress hormones may contribute to vaso-occlusion. ..
  38. MECHANISM OF ACTIVATION OF PLATELET GPIIB AND GPIIIA
    Jeffrey Smith; Fiscal Year: 2001
    ..Binding studies will be performed between Ca2+ and purified conformers of dormant and active IIb-IIIa. Results from this analysis should determine which class of ion binding sites regulate activation of the integrin. ..
  39. INTERACTION OF OSTEOPONTIN WITH ADHESION RECEPTORS
    JOEL BENNETT; Fiscal Year: 2002
    ..Our results suggest that it may be possible to inhibit the formation of platelet thrombi in arteries involved by atherosclerosis by preventing the interaction of platelet alphavbeta3 with osteopontin. ..
  40. Molecular Genetics of Integrin Collagen Receptors
    Thomas Kunicki; Fiscal Year: 2007
    ..From a clinical standpoint, these studies will also reveal the impact of these gene differences on risk for adverse events in bleeding disorders such as Von Willebrand Disease. ..
  41. MOLECULAR BIOLOGY OF COLLAGEN RECEPTORS
    Thomas Kunicki; Fiscal Year: 2004
    ..The successful completion of the proposed studies will lead to a substantial increase in our understanding of the genetics and physiology of the platelet collagen receptor, GPVI. ..
  42. MONITORING DISEASE AND THERAPY IN DYSTROPHIN-DEFICIENT MUSCLE USING ULTRASOUND
    MICHAEL SCOTT HUGHES; Fiscal Year: 2010
    ..The ultimate goal is to produce a comprehensive package for quantitative ultrasound tissue characterization of muscular dystrophy, which is fully validated by robust independent measures (e.g., strength testing) ..
  43. LYSOSOMES AND ATHEROSCLEROTIC FOAM CELL GENESIS
    WALTER JEROME; Fiscal Year: 2002
    ..Completion of the aims will significantly expand understanding of lysosomal FC and CE accumulation and provide information to begin determining the role of this accumulation in atherogenesis. ..
  44. The cGMP-dependent protein kinase pathway in platelets
    Xiaoping Du; Fiscal Year: 2009
    ..3.To characterize the roles and the signaling mechanisms of different isoforms of PKGin platelet secretion and aggregation. ..
  45. SHEAR STRESS MODULATION OF ENDOTHELIAL WOUND HEALING
    MARIA LUIZA ALBUQUERQUE; Fiscal Year: 2003
    ..Investigation of the mechanisms underlying endothelial repair during shear stress may lead to better strategies for the management of patients suffering from a variety of cardiovascular disorders. ..
  46. BIOFUNCTIONAL MATERIALS CONTAINING PROTEIN MIMETICS
    WEIYUAN KAO; Fiscal Year: 2003
    ..The controlled release of these bioactive factors may have therapeutic values in the fundamental processes of inflammation, biocompatibility, and tissue healing. ..
  47. OSTEOCLASTIC PHOSPHOTYROSYL PHOSPHATASE AND RESORPTION
    KIN HING LAU; Fiscal Year: 2002
    ....
  48. Dissection of cellular interactions with integrated functional genomics in comple
    Martin Hessner; Fiscal Year: 2008
    ..Understanding the relationship between mast cells, T effector, and TREG cells in autoimmunity and has the potential to establish a new paradigm for immune regulation and create new insights to human disease. ..
  49. Interaction between host cells and multicomponent material matrix
    WEIYUAN KAO; Fiscal Year: 2009
    ..e., human blood-derived macrophages, dermal fibroblasts, and keratinocytes) using monoculture and binary cell culture systems. (3) To ascertain IPN efficacy in vivo in modulating the host reaction and healing. ..
  50. Prenatal LPS-induced changes in gene expression
    Paul Carvey; Fiscal Year: 2005
    ..The results of these studies will also provide a unique opportunity to determine if prenatal neurotoxin exposure can permanently alter genes that increase the risk of neurodegenerative disease in later life. ..
  51. Multiplex Screening for ABC Transporter Inhibitors
    Richard Larson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  52. SPECIFIC TISSUE TARGETED ULTRASONIC CONTRAST AGENT
    Michael Hughes; Fiscal Year: 2004
    ....
  53. Vascular tropism of Borrelia burgdorferi
    Jenifer Coburn; Fiscal Year: 2005
    ..The experiments that we are proposing will shed light on the significance of three B. burgdorferi proteins that may facilitate interactions of B. burgdorferi with the vasculature during the natural course of infection in mammals. ..
  54. Neutralizing Compounds for Viral Hemorrhagic Fever
    Richard Larson; Fiscal Year: 2005
    ....
  55. 2006 Gordon Research Conference on Signal Transduction By Engineered ECM
    Michael Sheetz; Fiscal Year: 2006
    ..A special effort will be made to use the financial support requested to facilitate the attendance and participation of women and minorities in this meeting. [unreadable] [unreadable] [unreadable]..
  56. Mechanism of anti-phagocytosis by Yersinia pestis
    Kristiina Vuori; Fiscal Year: 2005
    ..We further expect that the inhibitors to be identified will be a valuable starting point for the further development of novel drugs that can be used to combat plague mortality. ..
  57. Regulation of Outside-In Integrin Signaling in Platelets
    SANFORD SHATTIL; Fiscal Year: 2007
    ..Thus, this line of investigation may lead to identification of new anti-thrombotic drug targets and serve as a paradigm for integrin signaling in other blood cells. [unreadable] [unreadable] [unreadable]..
  58. Role of IGF-II in Human Trophoblast-Decidua Interactions
    Linda Giudice; Fiscal Year: 2006
    ..The investigators work with the human model because of the uniqueness of implantation in humans...
  59. MODULATION OF ALPHA 5 BETA 1 INTEGRIN
    David Boettiger; Fiscal Year: 2007
    ..abstract_text> ..
  60. MECHANISM OF PLATELET ACTIVATION ON MATRIX PROTEINS
    Beatrice Haimovich; Fiscal Year: 2004
    ..phosphatase(s) and kinase(s) regulate the state of phosphorylation of a-actinin? Aim 3: What is the role of tyrosine phosphorylation of pplOl and vinculin, and which phosphatase(s) and kinase(s) regulate these phosphorylation events? ..
  61. Nanoparticle targeting of cathepsin-L inhibitor and doxorubicin in breast cancer
    Shaker Mousa; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..