cd34 antigens

Summary

Summary: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.

Top Publications

  1. ncbi Human embryonic stem cell-derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential
    Maxim A Vodyanik
    Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53792 8550, USA
    Blood 105:617-26. 2005
  2. ncbi AC133, a novel marker for human hematopoietic stem and progenitor cells
    A H Yin
    AmCell Corp, Sunnyvale, CA 94089, USA
    Blood 90:5002-12. 1997
  3. pmc Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells
    J R Beauchamp
    Muscle Cell Biology Group, Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, W12 ONN United Kingdom
    J Cell Biol 151:1221-34. 2000
  4. ncbi Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease
    M Vasa
    Division of Molecular Cardiology, Department of Internal Medicine IV, University of Frankfurt, Germany
    Circ Res 89:E1-7. 2001
  5. ncbi Circulating endothelial progenitor cells are reduced in peripheral vascular complications of type 2 diabetes mellitus
    Gian Paolo Fadini
    Department of Clinical and Experimental Medicine, Division of Metabolic Diseases, University of Padova, School of Medicine, Padova, Italy
    J Am Coll Cardiol 45:1449-57. 2005
  6. ncbi Dasatinib (BMS-354825) targets an earlier progenitor population than imatinib in primary CML but does not eliminate the quiescent fraction
    Mhairi Copland
    Division of Cancer Sciences and Molecular Pathology, University of Glasgow, UK
    Blood 107:4532-9. 2006
  7. ncbi Mobilization of endothelial progenitor cells in patients with acute myocardial infarction
    S Shintani
    Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, Japan
    Circulation 103:2776-9. 2001
  8. ncbi Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction
    Margherita Massa
    Biotechnology Laboratory, Department of Cardiology, Internal Medicine and Mecical Oncology, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
    Blood 105:199-206. 2005
  9. ncbi In vitro differentiation of endothelial cells from AC133-positive progenitor cells
    U M Gehling
    Department of Hematology Oncology, University Hospital Eppendorf, Hamburg, Germany
    Blood 95:3106-12. 2000
  10. ncbi Development of a human adaptive immune system in cord blood cell-transplanted mice
    Elisabetta Traggiai
    Institute for Research in Biomedicine IRB, Via Vela 6, 6500 Bellinzona, Switzerland
    Science 304:104-7. 2004

Research Grants

Detail Information

Publications258 found, 100 shown here

  1. ncbi Human embryonic stem cell-derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential
    Maxim A Vodyanik
    Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53792 8550, USA
    Blood 105:617-26. 2005
    ..These data indicate that CD34+ cells generated through hES/OP9 coculture display several features of definitive hematopoietic stem cells...
  2. ncbi AC133, a novel marker for human hematopoietic stem and progenitor cells
    A H Yin
    AmCell Corp, Sunnyvale, CA 94089, USA
    Blood 90:5002-12. 1997
    ....
  3. pmc Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells
    J R Beauchamp
    Muscle Cell Biology Group, Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, W12 ONN United Kingdom
    J Cell Biol 151:1221-34. 2000
    ..We conclude that the expression of CD34, Myf5, and M-cadherin defines quiescent, committed precursors and speculate that the CD34(-ve), Myf5(-ve) minority may be involved in maintaining the lineage-committed majority...
  4. ncbi Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease
    M Vasa
    Division of Molecular Cardiology, Department of Internal Medicine IV, University of Frankfurt, Germany
    Circ Res 89:E1-7. 2001
    ..The full text of this article is available at http://www.circresaha.org...
  5. ncbi Circulating endothelial progenitor cells are reduced in peripheral vascular complications of type 2 diabetes mellitus
    Gian Paolo Fadini
    Department of Clinical and Experimental Medicine, Division of Metabolic Diseases, University of Padova, School of Medicine, Padova, Italy
    J Am Coll Cardiol 45:1449-57. 2005
    ..We sought to establish whether a reduction in endothelial progenitor cells (EPCs) has a putative role in peripheral vascular disease (PVD) of type 2 diabetic patients...
  6. ncbi Dasatinib (BMS-354825) targets an earlier progenitor population than imatinib in primary CML but does not eliminate the quiescent fraction
    Mhairi Copland
    Division of Cancer Sciences and Molecular Pathology, University of Glasgow, UK
    Blood 107:4532-9. 2006
    ..These data confirm that dasatinib is more effective than IM within the CML stem cell compartment; however, the most primitive quiescent CML cells appear to be inherently resistant to both drugs...
  7. ncbi Mobilization of endothelial progenitor cells in patients with acute myocardial infarction
    S Shintani
    Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, Japan
    Circulation 103:2776-9. 2001
    ..35, P=0.01). CONCLUSIONS: This is the first clinical demonstration showing that lineage-committed EPCs and MNC(CD34+), their putative precursors, are mobilized during an acute ischemic event in humans...
  8. ncbi Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction
    Margherita Massa
    Biotechnology Laboratory, Department of Cardiology, Internal Medicine and Mecical Oncology, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
    Blood 105:199-206. 2005
    ..05). No significant difference was documented between patients with SA and CTRLs. Spontaneous mobilization of both HPCs and EPCs occurs within a few hours from the onset of AMI and is detectable until 2 months...
  9. ncbi In vitro differentiation of endothelial cells from AC133-positive progenitor cells
    U M Gehling
    Department of Hematology Oncology, University Hospital Eppendorf, Hamburg, Germany
    Blood 95:3106-12. 2000
    ..Whether these hematopoietic and endothelial progenitors develop from a common precursor, the hemangioblast will be studied at the single-cell level...
  10. ncbi Development of a human adaptive immune system in cord blood cell-transplanted mice
    Elisabetta Traggiai
    Institute for Research in Biomedicine IRB, Via Vela 6, 6500 Bellinzona, Switzerland
    Science 304:104-7. 2004
    ..This provides a valuable model to study development and function of the human adaptive immune system in vivo...
  11. ncbi Generalised reduction of putative endothelial progenitors and CXCR4-positive peripheral blood cells in type 2 diabetes
    C G Egan
    Department of Neuroscience, University of Siena, Via A Moro, 53100, Siena, Italy
    Diabetologia 51:1296-305. 2008
    ..Since expression of chemokine (C-X-C motif) receptor 4 (CXCR4) has been associated with mobilisation and recruitment of progenitor cells, CXCR4 expression was also analysed...
  12. ncbi Expression of the CD34 gene in vascular endothelial cells
    L Fina
    Leukaemia Research Fund Centre, Institute of Cancer Research, London, UK
    Blood 75:2417-26. 1990
    ..However, well-established endothelial cell contacts with tight junctions are CD34 negative. CD34 may function as an adhesion molecule on both endothelial cells and hematopoietic progenitors...
  13. ncbi Intramuscular transplantation of G-CSF-mobilized CD34(+) cells in patients with critical limb ischemia: a phase I/IIa, multicenter, single-blinded, dose-escalation clinical trial
    Atsuhiko Kawamoto
    Division of Vascular Regeneration Therapy, Department of Translational Research, Institute of Biomedical Research and Innovation, Kobe 650 0047, Japan
    Stem Cells 27:2857-64. 2009
    ..Favorable trends in efficacy parameters encourage a randomized and controlled trial in the future...
  14. pmc MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation
    Nadia Felli
    Department of Hematology, Oncology, and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy
    Proc Natl Acad Sci U S A 102:18081-6. 2005
    ..Furthermore, the results on kit+ erythroleukemic cells suggest a potential role of these miRs in cancer therapy...
  15. ncbi Tumor microvessel density as a predictor of recurrence after resection of hepatocellular carcinoma: a prospective study
    Ronnie Tung Ping Poon
    Department of Surgery and Pathology, University of Hong Kong Medical Center, Queen Mary Hospital, Hong Kong, China
    J Clin Oncol 20:1775-85. 2002
    ..This study prospectively evaluated the correlation of tumor microvessel density (MVD) with clinicopathologic features and postoperative recurrence in patients undergoing resection of hepatocellular carcinoma (HCC)...
  16. ncbi Selective survival of peripheral blood lymphocytes in children with HIV-1 following delivery of an anti-HIV gene to bone marrow CD34(+) cells
    Greg M Podsakoff
    Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
    Mol Ther 12:77-86. 2005
    ..These findings indicate that there was a selective survival advantage for PBMC containing the huM10 gene during the time of increased HIV-1 load...
  17. ncbi Polyamine depletion reduces TNFalpha/MG132-induced apoptosis in bone marrow stromal cells
    Claudio Muscari
    Department of Biochemistry G Moruzzi, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Stem Cells 23:983-91. 2005
    ..Thus, polyamine depletion can be considered a useful tool for counteracting programmed cell death in BMSCs without involving the p53 proapoptotic protein...
  18. pmc Self-renewal and expansion of single transplanted muscle stem cells
    Alessandra Sacco
    Baxter Laboratory in Genetic Pharmacology, Department of Microbiology and Immunology, Stem Cell Institute, Stanford University School of Medicine, Stanford, California 94305 5175, USA
    Nature 456:502-6. 2008
    ....
  19. ncbi Nitric oxide cytoskeletal-induced alterations reverse the endothelial progenitor cell migratory defect associated with diabetes
    Mark S Segal
    Division of Nephrology, Hypertension and Transplantation, University of Florida, P O Box 100224, Gainesville, FL 32610 0267, USA
    Diabetes 55:102-9. 2006
    ..This inability of diabetic EPCs to respond to SDF-1 may contribute to aberrant tissue vascularization and endothelial repair in diabetic patients...
  20. ncbi Prospective isolation of skeletal muscle stem cells with a Pax7 reporter
    Darko Bosnakovski
    Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA
    Stem Cells 26:3194-204. 2008
    ..These results establish a novel animal model for the study of muscle stem cell physiology and a culture system for expansion of engraftable muscle progenitors...
  21. pmc Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy
    Joseph S Anderson
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Retrovirology 3:24. 2006
    ....
  22. ncbi Glucose tolerance is negatively associated with circulating progenitor cell levels
    G P Fadini
    Department of Clinical and Experimental Medicine, Medical School, University of Padua, Via Giustiniani 2, 35100, Padua, Italy
    Diabetologia 50:2156-63. 2007
    ..This study was designed to evaluate progenitor cell levels in volunteers with different degrees of glucose tolerance...
  23. ncbi Chronic myeloid leukemia stem cells possess multiple unique features of resistance to BCR-ABL targeted therapies
    X Jiang
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
    Leukemia 21:926-35. 2007
    ..Taken together, these results provide the first evidence of multiple mechanisms of innate IM resistance in primitive and quiescent CML cells...
  24. ncbi Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4
    A Peled
    Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Science 283:845-8. 1999
    ..Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation...
  25. ncbi CD34+ fibrocytes in invasive ductal carcinoma, ductal carcinoma in situ, and benign breast lesions
    Peter J Barth
    Institute of Pathology, Philipps University, 35033 Marburg, Germany
    Virchows Arch 440:298-303. 2002
    ..These findings may be helpful tools in distinguishing benign breast lesions (e.g., sclerosing adenosis) from invasive breast cancer and in characterizing stromal remodeling associated with invasive cancer...
  26. ncbi Estimation of angiogenesis with anti-CD105 immunostaining in the process of colorectal cancer development
    Kazunari Akagi
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Surgery 131:S109-13. 2002
    ..We hypothesized that microvessel quantification by means of CD105 might be useful for measuring angiogenesis in the colorectal adenoma-carcinoma sequence...
  27. pmc Blood-derived angioblasts accelerate blood-flow restoration in diabetic mice
    G C Schatteman
    Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52245, USA
    J Clin Invest 106:571-8. 2000
    ..Our findings suggest that CD34(+) cells may be a useful tool for therapeutic angiogenesis in diabetics...
  28. ncbi Adult mouse hematopoietic stem cells: purification and single-cell assays
    Hideo Ema
    Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Protoc 1:2979-87. 2006
    ..Initialization of single-cell culture requires approximately 1 h. Single-cell transplantation requires approximately 6 h. Single-cell immunostaining requires approximately 2 d...
  29. ncbi The use of AMD3100 plus G-CSF for autologous hematopoietic progenitor cell mobilization is superior to G-CSF alone
    Neal Flomenberg
    Thomas Jefferson University, 125 S Ninth St, Ste 801 Sheridan Bldg, Philadelphia, PA 19107, USA
    Blood 106:1867-74. 2005
    ..All study objectives were met without significant toxicity. The results demonstrate that the combination of A + G is generally safe, effective, and superior to G alone for autologous HPC mobilization...
  30. ncbi Decreased number of circulating progenitor cells in obesity: beneficial effects of weight reduction
    Jochen Müller-Ehmsen
    Department of Internal Medicine III, University of Cologne, Kerpener Str 62, 50937 Cologne, Germany
    Eur Heart J 29:1560-8. 2008
    ..The aim of this study was to determine whether the number of CPC is an independent correlate of body mass index (BMI) and whether weight loss leads to an increase in CPC...
  31. ncbi Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell
    D S Krause
    Department of Laboratory Medicine and, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 105:369-77. 2001
    ..This finding may contribute to clinical treatment of genetic disease or tissue repair...
  32. ncbi Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells
    Regis Doyonnas
    The Biomedical Research Centre, University of British Columbia, 2222 Health Sciences Mall, Vancouver, Canada V6T 1Z3
    Blood 105:4170-8. 2005
    ..In summary, we find that podocalyxin is a marker of embryonic HSCs and erythroid cells and of adult HSCs and that it may be a valuable marker for the purification of these cells for transplantation...
  33. ncbi Chemokine SDF-1 enhances circulating CD34(+) cell proliferation in synergy with cytokines: possible role in progenitor survival
    J J Lataillade
    Laboratoire d Immunologie Cellulaire, centre de transfusion sanguine des armées Jean Julliard, Clamart Cedex, France
    Blood 95:756-68. 2000
    ..Furthermore, both adhesion-induced CXCR-4 overexpression and SDF-1 stimulating activity may be of clinical relevance for improving cell therapy settings in stem cell transplantation...
  34. pmc Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins
    J P Levesque
    Department of Immunology, Hanson Centre for Cancer Research, Adelaide, Australia
    J Exp Med 181:1805-15. 1995
    ....
  35. pmc Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development
    Azusa Matsubara
    Laboratory of Stem Cell Therapy, Center for Experimental Medicine, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    J Exp Med 202:1483-92. 2005
    ..Our findings establish Endomucin as a novel cell-surface marker for LTR-HSCs throughout development and provide a powerful tool in understanding HSC ontogeny...
  36. ncbi High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors
    Yue Ma
    National Primate Research Center, School of Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Stem Cells 21:111-7. 2003
    ..These results also have important implications for the possible future use of gene-modified human ES cells in transplantation and tissue regeneration applications...
  37. ncbi Increased circulating fibrocytes in asthma with chronic airflow obstruction
    Chun Hua Wang
    Department of Thoracic Medicine, Chang Gung Memorial Hospital, 199 Tun Hwa North Road, Taipei, Taiwan
    Am J Respir Crit Care Med 178:583-91. 2008
    ..We hypothesized that this effect may result from increased activity of circulating fibroblast-like progenitor cells (fibrocytes) that could home to the airway mucosal wall...
  38. ncbi SCID-repopulating cell activity of human cord blood-derived CD34- cells assured by intra-bone marrow injection
    Jianfeng Wang
    Department of Hygiene, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyoku, Kyoto, Japan
    Blood 101:2924-31. 2003
    ....
  39. ncbi Improvement of postnatal neovascularization by human adipose tissue-derived stem cells
    A Miranville
    Department of Cardiovascular Physiology, J W Goethe University, Frankfurt, Germany
    Circulation 110:349-55. 2004
    ..Several studies have suggested that stem cells are present in the stroma-vascular fraction (SVF) of adipose tissue (AT)...
  40. ncbi High-penetrance mouse model of acute promyelocytic leukemia with very low levels of PML-RARalpha expression
    Peter Westervelt
    Washington University, Division of Oncology, 660 S Euclid Ave, Campus Box 8007, St Louis, MO 63110 1093, USA
    Blood 102:1857-65. 2003
    ..Although the explanation for this result is not yet clear, one hypothesis suggests that very low levels of PML-RARalpha expression in early myeloid cells may be optimal for the development of APL in mice...
  41. pmc AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates
    Andre Larochelle
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10 CRC, Rm 4E 5132, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Blood 107:3772-8. 2006
    ..Thus, AMD3100-mobilized CD34(+) cells represent an alternative source of hematopoietic stem cells for clinical stem cell transplantation and genetic manipulation with integrating retroviral vectors...
  42. pmc Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells
    Neeltje A Kootstra
    Laboratory of Genetics and Infectious Disease Laboratory, The Salk Institute, La Jolla, CA 92037 1099, USA
    Proc Natl Acad Sci U S A 100:1298-303. 2003
    ..Overall, the findings demonstrate that the host range of HIV-1-based LV can be altered by modifications in the packaging construct...
  43. ncbi Reduced number of circulating endothelial progenitor cells predicts future cardiovascular events: proof of concept for the clinical importance of endogenous vascular repair
    Caroline Schmidt-Lucke
    Molecular Cardiology, Department of Medicine III, J W Goethe University, Frankfurt, Germany
    Circulation 111:2981-7. 2005
    ..Circulating EPCs may thus provide an endogenous repair mechanism to counteract ongoing risk factor-induced endothelial injury and to replace dysfunctional endothelium...
  44. ncbi Stromal cell-derived factor 1 regulates primitive hematopoiesis by suppressing apoptosis and by promoting G(0)/G(1) transition in CD34(+) cells: evidence for an autocrine/paracrine mechanism
    Jean Jacques Lataillade
    Laboratoire d Immunologie Cellulaire, centre de transfusion sanguine des armées Jean Julliard, Clamart Cedex, France
    Blood 99:1117-29. 2002
    ....
  45. ncbi Rapid mobilization of CD34+ cells following administration of the CXCR4 antagonist AMD3100 to patients with multiple myeloma and non-Hodgkin's lymphoma
    Steven M Devine
    Washington University School of Medicine, St Louis, MO 63110, USA
    J Clin Oncol 22:1095-102. 2004
    ..We performed a phase I study assessing the safety and clinical effects of AMD3100 in patients with multiple myeloma (MM) and non-Hodgkin's lymphoma (NHL)...
  46. ncbi Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro
    Annette M Müller
    Institute of Pathology, University Clinic Bergmannsheil, Ruhr University Bochum, Burkle de la Camp Platz 1, Bochum, 44789 Germany
    Exp Mol Pathol 72:221-9. 2002
    ..Moreover, the need for endothelial markers specific for single microvascular type ECs becomes obvious in order to establish cultures consisting of only one microvascular ECs subpopulation...
  47. ncbi CD34-positive cells exhibit increased potency and safety for therapeutic neovascularization after myocardial infarction compared with total mononuclear cells
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 114:2163-9. 2006
    ..We compared the therapeutic potential of purified mobilized human CD34+ cells with that of mobilized total mononuclear cells (tMNCs) for the preservation/recovery of myocardial tissue integrity and function after myocardial infarction (MI)...
  48. ncbi CD34 expression patterns during early mouse development are related to modes of blood vessel formation and reveal additional sites of hematopoiesis
    H B Wood
    Department of Human Anatomy, University of Oxford, UK
    Blood 90:2300-11. 1997
    ..These observations suggest functions for CD34 in relation to specific modes of blood vessel formation, and a hemangioblastic role in both embryonic and extraembryonic sites...
  49. ncbi Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells
    Maria M Ho
    Department of Cancer Genetics and Developmental Biology, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    Cancer Res 67:4827-33. 2007
    ..These findings indicate that SP is an enriched source of lung tumor-initiating cells with stem cell properties and may be an important target for effective therapy and a useful tool to investigate the tumorigenic process...
  50. pmc Essential and instructive roles of GATA factors in eosinophil development
    Ryutaro Hirasawa
    Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    J Exp Med 195:1379-86. 2002
    ..Taken together, our findings establish essential and instructive roles of GATA factors in eosinophil development. GATA-1 and GATA-2 could be novel molecular targets for therapeutic approaches to allergic inflammation...
  51. pmc Expression of Thy-1 on human hematopoietic progenitor cells
    W Craig
    Terry Fox Laboratory, British Columbia Cancer Agency, Departments of Medicine, Pathology, and Medical Genetics, University of British Columbia, Vancouver, Canada
    J Exp Med 177:1331-42. 1993
    ..Taken together our results indicate that Thy-1-specific antibody 5E10 is an attractive tool for further studies on the biology and purification of human stem cells...
  52. ncbi High incidence of microscopic gastrointestinal stromal tumors in the stomach
    Kaori Kawanowa
    Department of Pathology, Tokyo Metropolitan Komagome Hospital, Tokyo, Japan
    Hum Pathol 37:1527-35. 2006
    ..Further studies are required to clarify the genetic events responsible for the transformation of microscopic GISTs to clinical GISTs...
  53. ncbi Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach
    Hana Raslova
    INSERM Unité 790, Institut Gustave Roussy, 1 rue Camille Desmoulins, 94805 Villejuif, France
    Blood 109:3225-34. 2007
    ..Together, these results suggest that MK polyploidization per se does not regulate gene expression but is intrinsically included in the differentiation process...
  54. ncbi Circulating CD34+ cells, metabolic syndrome, and cardiovascular risk
    Gian Paolo Fadini
    Department of Clinical and Experimental Medicine, Division of Metabolic Diseases, University of Padua, Medical School, Padua, Italy
    Eur Heart J 27:2247-55. 2006
    ..As general agreement on their characterization is lacking, this work was carried out to assess the relationships between different antigenic profiles of progenitor cells and CV risk, with special regard to metabolic syndrome (MetSyn)...
  55. pmc Microenvironment determines lineage fate in a human model of MLL-AF9 leukemia
    Junping Wei
    Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Cancer Cell 13:483-95. 2008
    ..Targeting the Rac signaling pathway by pharmacologic or genetic means resulted in rapid and specific apoptosis of MLL-AF9 cells, suggesting that the Rac signaling pathway may be a valid therapeutic target in MLL-rearranged AML...
  56. ncbi Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells
    Maikel J Oedayrajsingh Varma
    Department of Plastic Surgery, VU University Medical Center, Amsterdam, The Netherlands
    Stem Cells Dev 16:91-104. 2007
    ....
  57. ncbi Investigating the feasibility of stem cell enrichment mediated by immobilized selectins
    Nichola Charles
    Department of Biomedical Engineering, University of Rochester, Rochester, New York, USA
    Biotechnol Prog 23:1463-72. 2007
    ..Optimal cell enrichment in immobilized selectin surfaces can be achieved within 10 min, much faster than most current commercially available systems...
  58. ncbi Erythropoietin regulates endothelial progenitor cells
    Ferdinand H Bahlmann
    Department of Internal Medicine, Hannover Medical School, Carl Neuberg Strasse 1, 30625, Hannover, Germany
    Blood 103:921-6. 2004
    ..Erythropoietin increases the number of functionally active EPCs in humans. Administration of rhEPO or EPO analogs may open new therapeutic strategies in regenerative cardiovascular medicine...
  59. pmc CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection
    Joseph Anderson
    Dept Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Retrovirology 2:53. 2005
    ..To extend these results to a stem cell gene therapy setting, here we show transduction of primary CD34+ hematopoietic progenitor cells to derive normal end stage cells that are resistant to HIV-1 infection...
  60. ncbi CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery
    Julie S Nielsen
    Trev and Joyce Deeley Research Centre, British Columbia Cancer Agency, Victoria, British Columbia, Canada
    Microcirculation 16:487-96. 2009
    ..Here, we review our recent studies, which suggest that CD34 does, indeed, play a role in leukocyte and HSC trafficking, but that this is through its action as a regulated blocker of cell adhesion and enhancer of migration...
  61. ncbi A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB)
    Gregorio Laino
    Dipartimento di Medicina Sperimentale, Sezione Istologia ed Embriologia, Secondo Ateneo di Napoli, Napoli, Italy
    J Bone Miner Res 20:1394-402. 2005
    ..This tissue constitute an ideal source of osteoblasts and mineralized tissue for bone regeneration. In fact, after in vivo transplantation into immunocompromised rats, LAB formed lamellar bone-containing osteocytes...
  62. pmc Therapeutic potential of vasculogenesis and osteogenesis promoted by peripheral blood CD34-positive cells for functional bone healing
    Tomoyuki Matsumoto
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047
    Am J Pathol 169:1440-57. 2006
    ..Our data suggest circulating human CD34+ cells have therapeutic potential to promote an environment conducive to neovascularization and osteogenesis in damaged skeletal tissue, allowing the complete healing of fractures...
  63. ncbi CD34 and CD43 inhibit mast cell adhesion and are required for optimal mast cell reconstitution
    Erin Drew
    The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Immunity 22:43-57. 2005
    ..Our data provide clear-cut evidence for a hematopoietic function for CD34 and suggest that it acts as a negative regulator of cell adhesion...
  64. ncbi Gastrointestinal stromal tumours
    M Miettinen
    Department of Soft Tissue Pathology, Armed Forces Institute of Pathology, Washington, DC, USA
    Ann Chir Gynaecol 87:278-81. 1998
    ....
  65. ncbi Transplantation of highly purified CD34+ progenitor cells from unrelated donors in pediatric leukemia
    Peter Lang
    Children s University Hospital, University of Tuebingen, Germany
    Blood 101:1630-6. 2003
    ..07). Transplantation of purified peripheral-blood CD34(+) cells from unrelated donors effectively minimizes GVHD and may be a good therapeutic option for patients with relapsed ALL...
  66. ncbi Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells
    D S Krause
    Experimental Hematopoiesis Program, Johns Hopkins Oncology Center, Baltimore, MD
    Blood 84:691-701. 1994
    ..These antibodies should be useful to select for murine hematopoietic stem cells...
  67. ncbi Expression of the miR-17-92 polycistron in chronic myeloid leukemia (CML) CD34+ cells
    Letizia Venturini
    Department of Hematology, Hemostasis, and Oncology, Hannover Medical School, Germany
    Blood 109:4399-405. 2007
    ..Altered miRNA expression may contribute to the pathophysiology of the disease and may provide potential targets for therapeutic intervention...
  68. ncbi Circulating CD34+ KDR+ endothelial progenitor cells correlate with erectile function and endothelial function in overweight men
    Katherine Esposito
    Department of Geriatrics and Metabolic Diseases, Division of Metabolic Diseases, Second University of Naples, Naples, Italy
    J Sex Med 6:107-14. 2009
    ..Bone marrow-derived endothelial progenitor cells (EPCs) circulate in the peripheral blood and are involved in endothelial homeostasis and repair...
  69. ncbi CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow
    Abraham Avigdor
    Immunology Department, The Weizmann Institute of Science, Rehovot, Israel
    Blood 103:2981-9. 2004
    ..Taken together, our data suggest a key role for CD44 and HA in SDF-1-dependent transendothelial migration of HSCs/HPCs and their final anchorage within specific niches of the BM...
  70. ncbi Evaluation of angiogenesis in non-small cell lung cancer: comparison between anti-CD34 antibody and anti-CD105 antibody
    F Tanaka
    Department of Thoracic Surgery, Faculty of Medicine, Kyoto University, Shogoin-kawahara-cho 54, Sakyo-ku, Kyoto 606-8507, Japan
    Clin Cancer Res 7:3410-5. 2001
    ..029), whereas CD34-IMVD was not (P = 0.070). CONCLUSIONS: Anti-CD105 mAb proved to be superior to anti-CD34 mAb in evaluation of angiogenesis in NSCLC...
  71. pmc Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma
    Lian Yue Yang
    Liver Cancer Laboratory, Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, PR China
    BMC Cancer 6:110. 2006
    ....
  72. ncbi Global gene expression profile of human cord blood-derived CD133+ cells
    Taina Jaatinen
    Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland
    Stem Cells 24:631-41. 2006
    ..This study provides a gene expression profile for human CD133+ cells. It presents a set of genes that may be used to unravel the properties of the CD133+ cell population, assumed to be highly enriched in HSCs...
  73. pmc Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist
    Hal E Broxmeyer
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Exp Med 201:1307-18. 2005
    ..These results support the hypothesis that the CXCL12-CXCR4 axis is involved in marrow retention of HSCs and HPCs, and demonstrate the clinical potential of AMD3100 for HSC mobilization...
  74. ncbi Tumor-infiltrating endothelial cells and endothelial precursor cells in inflammatory breast cancer
    Kazuo Shirakawa
    Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan
    Int J Cancer 99:344-51. 2002
    ..These ECs/EPCs incorporated acetylated lipoprotein and were integrated within a HUVEC monolayer in vitro culture on day 5...
  75. ncbi Constitutive activation of Flt3 and STAT5A enhances self-renewal and alters differentiation of hematopoietic stem cells
    Malcolm A S Moore
    Moore Laboratory, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Exp Hematol 35:105-16. 2007
    ..To model human leukemogenesis by transduction of human hematopoietic stem cells (HSC) with genes associated with leukemia and expressed in leukemic stem cells...
  76. ncbi Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood
    Rima Haddad
    EMI 0013 Institut National de la Santé et de la Recherche Médicale Université Paris 7, Laboratoire d Immunologie Cellulaire et Immunopathologie de l EcolePratique des Hauntes Etudes, Hopital Saint Louis, 75475 Paris Cedex 10, France
    Blood 104:3918-26. 2004
    ..These findings indicate that CD34(+)CD45RA(hi)CD7(+) and CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs correspond to multipotent early lymphoid progenitors polarized toward either the T/NK or B lineage, respectively...
  77. pmc Thrombomucin, a novel cell surface protein that defines thrombocytes and multipotent hematopoietic progenitors
    K M McNagny
    Cell Regulation Program, European Molecular Biology Laboratory, Heidelberg, D 69117 Germany
    J Cell Biol 138:1395-407. 1997
    ..They also suggest that thrombomucin and CD34 form a family of stem cell-specific proteins with possibly overlapping functions in early hematopoietic progenitors...
  78. pmc High-dose immunosuppressive therapy and autologous peripheral blood stem cell transplantation for severe multiple sclerosis
    Richard A Nash
    Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, D1 100, PO Box 19024, Seattle, WA 98109 1024, USA
    Blood 102:2364-72. 2003
    ..This was a heterogeneous high-risk group, and a phase 3 study is planned to fully assess efficacy...
  79. pmc Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood
    Sonja Kimmig
    Department of Clinical Immunology, Deutsches Rheuma ForschungsZentrum Berlin, Schumannstrasse 21 22, 10117 Berlin, Germany
    J Exp Med 195:789-94. 2002
    ..We describe here the identification of peripherally expanded naive Th cells in human adult blood characterized by the loss of CD31 expression and a highly reduced TREC content...
  80. ncbi Transcriptional profiling of human hematopoiesis during in vitro lineage-specific differentiation
    Martina Komor
    Department of Hematology, Oncology and Transfusion Medicine, University Hospital Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Germany
    Stem Cells 23:1154-69. 2005
    ..The expression of selected genes was confirmed by real-time polymerase chain reaction. Our data encompass the first extensive transcriptional profile of human hematopoiesis during in vitro lineage-specific differentiation...
  81. ncbi Reciprocal activation of GATA-1 and PU.1 marks initial specification of hematopoietic stem cells into myeloerythroid and myelolymphoid lineages
    Yojiro Arinobu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell Stem Cell 1:416-27. 2007
    ..1 primarily organizes the hematopoietic lineage fate decision to form the earliest hematopoietic branchpoint that comprises isolatable myeloerythroid and myelolymphoid progenitor populations...
  82. pmc Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34
    Robert W Storms
    Department of Medicine, Division of Cellular Therapy, Duke University Medical Center, Box 3361, Durham, NC 27710, USA
    Blood 106:95-102. 2005
    ..The differential expression of ALDH may provide a means to explore normal and malignant processes associated with myeloid and lymphoid development...
  83. pmc Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation
    Jan Jacob Schuringa
    Laboratory of Developmental Hematopoiesis, Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    J Exp Med 200:623-35. 2004
    ..Our data indicate that a persistent activation of STAT5A in human hematopoietic stem and progenitor cells results in their enhanced self-renewal and diverts differentiation to the erythroid lineage...
  84. ncbi Functional isolation and characterization of human hematopoietic stem cells
    A C Berardi
    Division of Hematology Oncology, Deaconess Hospital, Harvard Medical School, Boston, MA 02215
    Science 267:104-8. 1995
    ..Thus, this technically simple strategy led to the efficient purification of cells with characteristics of hematopoietic stem cells...
  85. ncbi Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells
    D L Simmons
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
    J Immunol 148:267-71. 1992
    ..CD34 has no significant sequence homology to any known protein but has some structural similarities to the heavily glycosylated leukocyte surface molecule CD43...
  86. ncbi Inhibition of HIV-1 by lentiviral vector-transduced siRNAs in T lymphocytes differentiated in SCID-hu mice and CD34+ progenitor cell-derived macrophages
    Akhil Banerjea
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1619 Campus Drive, Fort Collins, Colorado 80523, USA
    Mol Ther 8:62-71. 2003
    ..These experiments demonstrate the utility of siRNAs delivered into hematopoietic stem cells via lentiviral vectors for future in vivo applications...
  87. ncbi Nonexpanded primary lung and bone marrow-derived mesenchymal cells promote the engraftment of umbilical cord blood-derived CD34(+) cells in NOD/SCID mice
    Pieternella S In 't Anker
    Departments of Obstetrics and Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 31:881-9. 2003
    ..The high frequency of MSC in fetal lung allowed us to study whether this represented a biological feature of these cells or a property that was acquired during expansion in culture...
  88. ncbi The human embryo, but not its yolk sac, generates lympho-myeloid stem cells: mapping multipotent hematopoietic cell fate in intraembryonic mesoderm
    M Tavian
    INSERM U506, Hopital Paul Brousse, 94807 Villejuif Cedex, France
    Immunity 15:487-95. 2001
    ..By contrast, hematopoietic cells arising in the third week yolk sac, as well as their progeny at later stages, were restricted to myelopoiesis and therefore are unlikely to contribute to definitive hematopoiesis in man...
  89. ncbi Dengue 2 virus inhibits in vitro megakaryocytic colony formation and induces apoptosis in thrombopoietin-inducible megakaryocytic differentiation from cord blood CD34+ cells
    Atanu Basu
    National Institute of Virology ICMR, Pune, India
    FEMS Immunol Med Microbiol 53:46-51. 2008
    ..These events might contribute towards the origin of thrombocytopenia in dengue disease...
  90. pmc Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin
    R Doyonnas
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC, Canada
    J Exp Med 194:13-27. 2001
    ..Our results provide the first example of an essential role for a sialomucin in development and suggest that defects in podocalyxin could play a role in podocyte dysfunction in renal failure and omphalocele in humans...
  91. ncbi Human adaptive immune system Rag2-/-gamma(c)-/- mice
    Laurie Chicha
    Department of Medicine II, Eberhard Karls University Medical School, Otfried Mueller Str 10, 72076 Tubingen, Germany
    Ann N Y Acad Sci 1044:236-43. 2005
    ....
  92. ncbi Pre-clinical development of cord blood-derived progenitor cell graft expanded ex vivo with cytokines and the polyamine copper chelator tetraethylenepentamine
    T Peled
    Gamida Cell Ltd, Jerusalem, Israel
    Cytotherapy 6:344-55. 2004
    ..This study extends our previous findings that copper chelation can modulate the balance between self-renewal and differentiation of hematopoietic progenitor cells...
  93. pmc Making eosinophils through subtle shifts in transcription factor expression
    Kelly McNagny
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    J Exp Med 195:F43-7. 2002
  94. ncbi Maintaining the self-renewal and differentiation potential of human CD34+ hematopoietic cells using a single genetic element
    James C Mulloy
    Cincinnati Children s Hospital Medical Center, 3333 Burnet Ave, Mail Location 7013, Cincinnati, OH 45229, USA
    Blood 102:4369-76. 2003
    ....
  95. ncbi Transdifferentiation of human peripheral blood CD34+-enriched cell population into cardiomyocytes, endothelial cells, and smooth muscle cells in vivo
    Edward T H Yeh
    Department of Cardiology, The University of Texas M D Anderson Cancer Center, Houston, Tex, USA
    Circulation 108:2070-3. 2003
    ..We investigated whether these cells could also transdifferentiate into human cardiomyocytes, mature endothelial cells, and smooth muscle cells in vivo...
  96. pmc The pattern of gene expression in human CD34(+) stem/progenitor cells
    G Zhou
    Department of Medicine, University of Chicago Medical Center, 5841 South Maryland Avenue, MC2115, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 98:13966-71. 2001
    ....
  97. ncbi Expression of functional CXCR4 by muscle satellite cells and secretion of SDF-1 by muscle-derived fibroblasts is associated with the presence of both muscle progenitors in bone marrow and hematopoietic stem/progenitor cells in muscles
    Mariusz Z Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Stem Cells 21:363-71. 2003
    ....
  98. ncbi CD34+ selected autologous peripheral blood stem cell transplantation for multiple sclerosis: report of toxicity and treatment results at one year of follow-up in 15 patients
    Enric Carreras
    BMT Unit, Hospital Clinic, Villarroel 170, 08036 Barcelona, Spain
    Haematologica 88:306-14. 2003
    ..We report the results of a phase II trial to evaluate feasibility and toxicity of CD34+ selected ASCT (CD34+/ASCT) and treatment results at one year of follow-up...
  99. ncbi Vascular endothelial growth factor expression in untreated osteosarcoma is predictive of pulmonary metastasis and poor prognosis
    M Kaya
    Department of Orthopedic Surgery, Sapporo Medical University School of Medicine, Hokkaido, Japan
    Clin Cancer Res 6:572-7. 2000
    ....
  100. ncbi Breast cancer resistance protein in drug resistance of primitive CD34+38- cells in acute myeloid leukemia
    Marc H G P Raaijmakers
    Department of Hematology and Central Hematology Laboratory, University Medical Center Nijmegen, St Radboud, 6500 HB Nijmegen, The Netherlands
    Clin Cancer Res 11:2436-44. 2005
    ....
  101. ncbi Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells
    C Gemelli
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy
    Cell Death Differ 13:1686-96. 2006
    ..Altogether our data allow to conclude that MafB is a key regulator of human monocytopoiesis...

Research Grants63

  1. System for Rapid Generation of Aptamers for Transplant Therapies
    KAREN QIAN; Fiscal Year: 2010
    ....
  2. Targeting Leukemic Stem Cells
    Daniel G Tenen; Fiscal Year: 2012
    ..Accomplishment of these goals will set the stage for clinical trials, as well as lead to development of therapies directed against other oncogenes as well as altering stem cell gene expression. ..
  3. UMBILICAL CORD STEM CELL CRYOPRESERVATION
    John Critser; Fiscal Year: 2000
    ..We expect that optimal cryopreservation procedures for umbilical cord derived stem cells will be different from those current used for bone marrow derived stem cells. ..
  4. Characterization of Hematopoietic Stem Cells
    Abba Zubair; Fiscal Year: 2007
    ..The overall goal of my studies is to improve the long-term clinical results of autologous HSCT in patients with hematological malignancies. ..
  5. FUNCTION OF CD34 STEM/PROGENITOR CELLS
    Saul Sharkis; Fiscal Year: 2002
    ..We expect that the results of our studies will be directly applicable to treatment of hematological disorders, genetic diseases, and malignancy. ..
  6. REGULATION OF THE HUMAN CD34 STEM CELL GENE
    Daniel Tenen; Fiscal Year: 2007
    ..1 To use CD34 regulatory elements expressing Cre recombinase and the tetracycline transactivator to enable tissue specific, regulatable expression of heterologous genes in the stem cell compartment. ..
  7. Dendritic Cells and Breast Cancer Immunity
    A Palucka; Fiscal Year: 2004
    ..Overall, this proposal will permit us to demonstrate that efficient breast cancer immunity can be generated and used to reject the tumor thus permitting development of novel treatment modalities in breast cancer. ..
  8. Mice with Human Dendritic Cells to Test Vaccine Potency
    A Palucka; Fiscal Year: 2004
    ..The experiments proposed here would lay ground for further use of SCID-hu/DCs mice to study other vaccines and demonstrate development of protective immunity upon in vivo rechallenge. ..
  9. Assessing Circulating Progenitor Cells in Heart Disease
    Thomas Povsic; Fiscal Year: 2007
    ..This study will look at two methods of measuring these stem cells in the blood of heart patients. These methods may someday be used to predict who will or will not develop heart disease or other complications. ..
  10. CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMING
    Hal Broxmeyer; Fiscal Year: 2005
    ..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
  11. FACTORS INFLUENCING STEM CELL AND PLATELET PRODUCTION
    Malcolm Moore; Fiscal Year: 2002
    ..e., adult populations acquiring features of neonatal cells, or whether there is a tendency for "immortalized" cells to undergo neoplastic transformation. ..
  12. Development of SL-101, An Immunotoxin that Targets Cancer Stem Cells
    Malcolm Moore; Fiscal Year: 2006
    ..This Phase I STTR grant will cover the development of SL-101 through key phases of pre-clinical development. [unreadable] [unreadable] [unreadable]..
  13. SUBPOPULATIONS OF HUMAN DENDRITIC CELLS
    Jacques Banchereau; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  14. Phase 3 stem cell transplant for light chain amyloidosis
    Morie A Gertz; Fiscal Year: 2011
    ..In addition, the analysis of clonal precursor B cells in the periphery and kinetics of amyloid formation of the monoclonal light chain will allow us to define and determine the mechanisms of disease progression and response to therapy. ..
  15. Facilitating Cells for Fetal Hematopoietic Transplant
    Laurence Shields; Fiscal Year: 2005
    ..Future directions will be towards the use of this model to explore the role and function of developmentally regulated adhesion molecules and their receptors as they relate to fetal hematopoietic transplantation. ..
  16. Altered CD8+ T cell Compartment in SLE Patients
    JACQUES F BANCHEREAU; Fiscal Year: 2010
    ..These studies will help us conclude whether killer CD8+T cells and/or suppressor CD8+ T cells i) can be used as markers and/or predictors of disease activity, ii) represent a valuable new option in lupus therapy. ..
  17. Developmental Checkpoints of Murine Mast Cells
    Koichi Akashi; Fiscal Year: 2009
    ..Results obtained from this project will be critical to study mast cell biology, which eventually will help develop new therapeutic strategies for a variety of allergic and autoimmune disorders. ..
  18. In Utero Stem Cell Transplantation for Hemophilia A
    Graca Almeida Porada; Fiscal Year: 2006
    ..abstract_text> ..
  19. Nonmyeloablative Hematopoietic Cell Transplantation for Severe Systemic Sclerosis
    Richard Nash; Fiscal Year: 2006
    ..The unique observations in this study regarding responses and recovery of organs after inducing a sustained remission of SSc will serve to focus and direct other non-transplant research activities. [unreadable] [unreadable] [unreadable]..
  20. Protection from GVHD with Gene-Modified Donor T Cells
    Richard Nash; Fiscal Year: 2008
    ....
  21. Lineage decision in blood stem and progenitor cells
    Koichi Akashi; Fiscal Year: 2007
    ..Thus, we hope these studies will help understand mechanistic effects of these transcription factors on lineage decision in normal and malignant hematopoietic development. ..
  22. Lung Transplantation and Immune Tolerance in Young Recipients
    Richard Nash; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  23. ENGRAFTING SENSITIZED HOSTS WITH NONABLATIVE REGIMENS
    Richard Nash; Fiscal Year: 2002
    ..By lessening the morbidity and mortality associated with conventional conditioning, these studies could significantly change the management of selected inherited red blood cell diseases. ..
  24. Dendritic Cell Vaccine in Metastatic Prostate Cancer
    Jacques Banchereau; Fiscal Year: 2002
    ..This study will permit us to establish the safety and tolerability of DCs loaded with killed allogeneic tumor cells as a source of TAA. It will also permit us to gain insights into the level of prostate cancer immunity induced by DCs. ..
  25. Dendritic Cells, Heat Shock Proteins and Tumor Immunity
    Jacques Banchereau; Fiscal Year: 2004
    ..These studies will permit us to produce an altered melanoma cell line, with enhanced immunogenicity, that will be used to generate tumor cell bodies to load onto DCs for vaccination protocols. ..
  26. PATHOBIOCHEMISTRY OF ACUTE PROMYELOCYTIC LEUKEMIA
    Edward Yeh; Fiscal Year: 2004
    ..These studies should provide novel insights into the mechanism and function of sentrinization and increase our understanding of the pathogenesis of acute promyelocytic leukemia. ..
  27. Evaluation of Stem Cell Plasticity In Vivo
    Graca Almeida Porada; Fiscal Year: 2004
    ..With these studies we hope to provide novel insights into the location of latent stem cell poois within the body and define the behavior and capabilities of these cells that are potentially of great clinical value. ..
  28. Acquisition of FACSVantage SE Flow Cytometer
    Yair Gazitt; Fiscal Year: 2004
    ..The new equipment will also widen the user's base, save sort time, reduce costs for users and will render sorting affordable to new users, especially in cases where low frequency (<1%) cell populations are sorted. ..
  29. Differentiation of Glioma from Radiation Injury Using Cellular MRI
    Syed Ali; Fiscal Year: 2008
    ..This magnetic labeling technique will also help investigators to track the injected CTL in the body as well as in the targeted areas by magnetic resonance imaging (MRI). [unreadable] [unreadable] [unreadable]..
  30. Epithelial engraftment of bone marrow derived stem cells
    Diane Krause; Fiscal Year: 2008
    ..The data obtained will lead us to design novel therapeutic strategies tissue injury and disease. [unreadable] [unreadable] [unreadable]..
  31. LEUKEMIA THERAPY WITH MIHA SPECIFIC T CELLS
    Wei Chen; Fiscal Year: 2002
    ..3. To develop methods develops for in vitro induction of MiHA-specific T cells for leukemia therapy. ..
  32. NATIONAL GENE VECTOR LABORATORY AT INDIANA UNIVERSITY
    Kenneth Cornetta; Fiscal Year: 2007
    ..abstract_text> ..
  33. EVOLUTION OF B PRECURSOR LEUKEMIA IN THE EU-RET MOUSE
    Stephan Grupp; Fiscal Year: 2003
    ..He will immunoprecipitate Rfp-Ret from the pro B cell populations of fetal and healthy adult E(-ret mice as well as from leukemias and compare the levels of tyrosine phophorylation of Rfp-Ret and Rfp-Ret-bound proteins. ..
  34. AUTOMATED CLOSED SYSTEM FOR DMSO REMOVAL FROM PERIPHERAL BLOOD STEM CELLS
    ERIK WOODS; Fiscal Year: 2008
    ..The goal of this research is to produce a device to easily remove DMSO from PBSC before they are transplanted, increasing effectiveness of the cells and reducing potential complications. [unreadable] [unreadable] [unreadable]..
  35. ADHESION ANALYSIS OF CYCLING STEM CELLS THROUGH ONTOGENY
    EDWARD SROUR; Fiscal Year: 2002
    ..Examination of these goals will contribute to our understanding of normal hematopoiesis and the mechanisms that govern homing of HSC and engraftment potential of candidate populations of stem cells. ..
  36. CONTROL SELECTION METHODS FOR CHILDHOOD CANCER STUDIES
    Greta Bunin; Fiscal Year: 2009
    ..A feasible and valid control group is needed to ensure that studies of childhood cancer are scientifically valid and therefore, will provide results that can eventually lead to prevention of these cancers. ..
  37. Cell Therapy of Refractory Leukemia
    Dario Campana; Fiscal Year: 2010
    ..The research proposed should not only spur clinical studies of immune cells in patients with refractory ALL but also facilitate the development of effective cell therapies for other neoplasms. ..
  38. The Role of CBFb-MYH11 in Acute Myeloid Leukemia
    James C Mulloy; Fiscal Year: 2010
    ..To identify specific signals that will cooperate in the transformation to acute leukemia, defined eenetic elements and saturatine retroviral mutaeenesis will be used. ..
  39. Extrinsic Modulation of Hematopoietic Stem Cell Fate
    Edward F Srour; Fiscal Year: 2010
    ..These studies are critical for assessing the impact of the interaction of stem cells with their hematopoietic microenvironment on stem cell function throughout ontogeny. ..
  40. Dental Pulp Derived Stem Cells: Optimizing Collection and Cryopreservation
    ERIK WOODS; Fiscal Year: 2008
    ..This proposed project would optimize methods for processing and freezing these cells for ease of distribution for research, testing, and eventually clinical use. [unreadable] [unreadable] [unreadable]..
  41. Barriers to Allogeneic Hematopoietic Stem Cell Engraftment
    JUDITH ANNE SHIZURU; Fiscal Year: 2010
    ..Based upon our understanding of the immune and non- immune barriers to engraftment we have a long-term goal of making allogeneic HCT safer and more effective for the treatment of malignant and non-malignant disease. ..
  42. The Role of MLL-AF9 in Acute Myeloid Leukemia
    James C Mulloy; Fiscal Year: 2010
    ..RELEVANCE (See instructions): We have recently shown that human blood stem cells can be induced to make leukemia upon introduction ..
  43. Nonablative AlloSCT in Refractory Autoimmune Disease
    Mitchell Cairo; Fiscal Year: 2003
    ....
  44. Targeting FLT3 as a Novel Specific Therapy for Leukemia
    Mark Levis; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  45. Transgenic Mouse Model for Barrett's Esophagus
    George Cotsarelis; Fiscal Year: 2003
    ..This would be the first transgenic inducible injury model that mimics Barrett's esophagus. It should be a valuable resource for those interested in studying the pathogenesis, chemoprevention and treatment of Barrett's esophagus. ..
  46. Hematopoietic cell Transplants for Autoimmune Diseases
    Judith Shizuru; Fiscal Year: 2005
    ..A further goal of this application is to understand the mechanism(s) by which the proposed autologous and allogeneic HCT regimens alter immune responses to autoantigens. ..
  47. PATERNAL EXPOSURE AND SPORADIC BILATERAL RETINOBLASTOMA
    Greta Bunin; Fiscal Year: 2005
    ..Sporadic heritable RBL is a childhood cancer worth studying in its own right and an ideal model for the investigation of new germline mutation. ..
  48. Role of AML1/ETO in Hematopoiesis and Leukemogenesis
    JAMES MULLOY; Fiscal Year: 2005
    ..The establishment of a small animal model of AML will greatly enhance our ability to develop and test treatment strategies and drugs that may be useful in the therapy of AML. ..
  49. MECHANISM OF HIV DNA INTEGRATION
    Frederic Bushman; Fiscal Year: 2005
    ..Long term, these studies, together with data on protein structures, will provide a high-resolution picture of the organization and function of preintegration complexes. ..
  50. Common Muscle, Hematopoietic and Neural Stem Cells
    EDWARD SROUR; Fiscal Year: 2004
    ....
  51. Nicotine Regulation of T Cell Development
    RONALD LUKAS; Fiscal Year: 2006
    ..One of the biomedically-relevant hypotheses of this project is that tobacco use and nicotine exposure affect immune system development. ..
  52. IN UTERO GENE THERAPY
    Christopher Porada; Fiscal Year: 2006
    ..These studies in this unique fetal model may lay the groundwork for devising clinically relevant in itero GT approaches for treating a variety of genetic diseases in human fetuses early in gestation, prior to disease onset. ..
  53. VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBC
    Stephen Wagner; Fiscal Year: 2004
    ..abstract_text> ..
  54. T(15;19) in Aggressive Pediatric Carcinoma
    Christopher French; Fiscal Year: 2006
    ..These studies will also reveal the range of primary sites from which t(15;19)+ carcinomas arise. ..
  55. Beta CELL PROGENITORS AND BONE MARROW MICROENVIRONMENT
    Dario Campana; Fiscal Year: 2004
    ..The proposed studies will further characterize the antileukemic activity of BAY 36-1677 and identify synergistically interacting compounds. ..
  56. Clinical Trial Planning Grant: Lentiviral Vector for HIV-1
    Donald Kohn; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  57. Prognostic Modeling of High-Risk Primary Breast Cancer
    Yago Nieto; Fiscal Year: 2003
    ..abstract_text> ..
  58. MOLECULAR REGULATION OF HUMAN MELANOMA METASTASIS
    Rakesh Singh; Fiscal Year: 2007
    ..We anticipate that the knowledge gained from these studies will identify new therapeutic targets for inhibiting the ligand binding and/or signal transduction events and the development of therapeutics for malignant melanoma. ..
  59. In Vivo BM Stem Cell Gene Transfer for MPS type I
    Dao Pan; Fiscal Year: 2006
    ..Taken together, these data would not only open a door to a novel approach for treatment of human diseases, but also provide a new tool to study adult stem cell plasticity and the nature of hematopoiesis. ..
  60. A NOVEL FROZEN STORAGE SYSTEM FOR CORD BLOOD STEM CELLS
    ERIK WOODS; Fiscal Year: 2004
    ....
  61. Maximizing CD8 T Cells for Protection
    David Masopust; Fiscal Year: 2009
    ..These findings will have direct relevance to vaccination and to establishing correlates of CD8 T cell dependent protective immunity. ..