Genomes and Genes
Summary: Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
Publications232 found, 100 shown here
- Structural basis of pore formation by the bacterial toxin pneumolysinSarah J Tilley
School of Crystallography and Institute of Structural Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
Cell 121:247-56. 2005..With an oligomer size of up to 44 subunits in the pore, this assembly creates a transmembrane channel 260 A in diameter lined by 176 beta strands...
- Recognition of pneumolysin by Toll-like receptor 4 confers resistance to pneumococcal infectionRichard Malley
Channing Laboratory, Brigham and Women s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:1966-71. 2003..We conclude that the interaction of pneumolysin with TLR4 is critically involved in the innate immune response to pneumococcus...
- The NLRP3 inflammasome is differentially activated by pneumolysin variants and contributes to host defense in pneumococcal pneumoniaMartin Witzenrath
Division of Infectious Diseases and Pulmonary Medicine, Department of Internal Medicine, Charite University Medicine Berlin, 13353 Berlin, Germany
J Immunol 187:434-40. 2011..The study indicates that a virulence factor polymorphism may substantially affect the recognition of bacteria by the innate immune system...
- Pneumolysin: a double-edged sword during the host-pathogen interactionHelen M Marriott
Section of Infection, Inflammation and Immunity, University of Sheffield School of Medicine and Biomedical Sciences, Sheffield, UK
Curr Mol Med 8:497-509. 2008....
- Streptolysin O clearance through sequestration into blebs that bud passively from the plasma membranePeter A Keyel
Howard Hughes Medical Institute, St Louis, MO 63110, USA
J Cell Sci 124:2414-23. 2011..We conclude, therefore, that ectocytosis is an important means for SLO clearance and hypothesize that this is a primary method by which cells defend themselves generally against pore-forming toxins...
- Early biofilm formation on microtiter plates is not correlated with the invasive disease potential of Streptococcus pneumoniaeAnel Lizcano
Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
Microb Pathog 48:124-30. 2010..In summary, these studies suggest that the ability to form early biofilms in vitro does not reflect virulence potential. More complex studies are required to determine if biofilm formation is important for virulence...
- Active Immunization with Pneumolysin versus 23-Valent Polysaccharide Vaccine for Streptococcus pneumoniae KeratitisErin W Norcross
Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
Invest Ophthalmol Vis Sci 52:9232-43. 2011..The purpose of this study was to determine whether active immunization against pneumolysin (PLY), or polysaccharide capsule, protects against the corneal damage associated with Streptococcus pneumoniae keratitis...
- Neutralizing antibodies elicited by a novel detoxified pneumolysin derivative, PlyD1, provide protection against both pneumococcal infection and lung injuryDanielle Salha
Sanofi Pasteur, Analytical Research and Development, Toronto, Ontario, Canada
Infect Immun 80:2212-20. 2012..Our detoxified PlyD1 antigen elicits such PLY neutralizing antibodies, thus serving as a candidate vaccine antigen for the prevention of pneumococcal pneumonia...
- Construction and immunological characterization of a novel nontoxic protective pneumolysin mutant for use in future pneumococcal vaccinesLea Ann S Kirkham
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
Infect Immun 74:586-93. 2006..Vaccination with this mutant protects mice from challenge with S. pneumoniae. Incorporation of this mutant pneumolysin into current pneumococcal vaccines may increase their efficacy...
- Pneumolysin localizes to the cell wall of Streptococcus pneumoniaeKatherine E Price
Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
J Bacteriol 191:2163-8. 2009..Hemolytic assays revealed that this cell wall-localized Ply is active. Additionally, cell wall-localized Ply is accessible to extracellular protease and is detergent releasable...
- Cholesterol-dependent actin remodeling via RhoA and Rac1 activation by the Streptococcus pneumoniae toxin pneumolysinAsparouh I Iliev
Cell Biophysics Group, European Neuroscience Institute Göttingen, Waldweg 33, 37073 Gottingen, Germany
Proc Natl Acad Sci U S A 104:2897-902. 2007..Thus, that our experiments show small GTPase activation by a cholesterol-dependent cytolysin suggests a membrane cholesterol-dependent activation mechanism...
- Pneumococcal pneumolysin and H(2)O(2) mediate brain cell apoptosis during meningitisJohann S Braun
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
J Clin Invest 109:19-27. 2002..Two bacterial toxins, pneumolysin and, to a lesser extent, H(2)O(2), induce apoptosis by translocation of AIF, suggesting new neuroprotective strategies for pneumococcal meningitis...
- Epithelial cells are sensitive detectors of bacterial pore-forming toxinsAdam J Ratner
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 281:12994-8. 2006..Osmosensing may thus represent a novel innate immune response to a common bacterial virulence strategy...
- The role of pneumolysin in pneumococcal pneumonia and meningitisR A Hirst
Department of Infection, Inflamation and Immunity, University of Leicester, Leicester, UK
Clin Exp Immunol 138:195-201. 2004..One of the main toxins released from pneumococci is the membrane pore forming toxin pneumolysin. Here we review the extensive studies on the role of pneumolysin in the pathogenesis of pneumococcal diseases...
- Pneumolysin-induced CXCL8 production by nasopharyngeal epithelial cells is dependent on calcium flux and MAPK activation via Toll-like receptor 4Semih Dogan
School of Clinical Sciences, University of Bristol, Bristol, UK
Microbes Infect 13:65-75. 2011..This may be important in regulating nasopharyngeal immunity against pneumococcal colonization...
- The conserved undecapeptide shared by thiol-activated cytolysins is involved in membrane bindingT Jacobs
Molecular Immunology, GBF National Center for Biotechnology, Mascheroder Weg 1, D 38124, Braunschweig, Germany
FEBS Lett 459:463-6. 1999..These results confirm the model of cytolysin binding suggested by structural data...
- Upper and lower respiratory tract infection by Streptococcus pneumoniae is affected by pneumolysin deficiency and differences in capsule typeAras Kadioglu
Department of Microbiology and Immunology, University of Leicester, Leicester, LE1 United Kingdom
Infect Immun 70:2886-90. 2002..The combination of capsule type and genetic background also determined virulence. Thus, the wild-type serotype 3 strain was virulent, whereas the capsule-switched mutant was avirulent...
- NAD+-glycohydrolase acts as an intracellular toxin to enhance the extracellular survival of group A streptococciAngela L Bricker
Channing Laboratory, 181 Longwood Avenue, Boston, MA 02115, USA
Mol Microbiol 44:257-69. 2002..We conclude that NAD+-glycohydrolase is a novel type of bacterial toxin that acts intracellularly in the infected host to enhance the survival and proliferation of an extracellular pathogen...
- Role of pneumolysin for the development of acute lung injury in pneumococcal pneumoniaMartin Witzenrath
Charite Universitatsmedizin Berlin, Department of Internal Medicine Infectious Diseases and Pulmonary Medicine, Berlin, Germany
Crit Care Med 34:1947-54. 2006..In a mouse model, we observed early-onset lung microvascular leakage after pulmonary infection with Streptococcus pneumoniae, and we hypothesized that the important virulence factor pneumolysin may be the direct causative agent...
- Hyaluronidase augments pneumolysin-mediated injury to human ciliated epitheliumC Feldman
Division of Pulmonology, Department of Medicine, Johannesburg Hospital and University of the Witwatersrand, 7 York Road, Parktown, 2193 Johannesburg, South Africa
Int J Infect Dis 11:11-5. 2007..The main objective of this study was to investigate the effects of pneumococcal hyaluronidase (0.1-10microg/ml), alone and in combination with pneumolysin (50 and 100ng/ml), on human ciliated epithelium...
- Streptolysin O inhibits clathrin-dependent internalization of group A StreptococcusLauren K Logsdon
Division of Infectious Diseases, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, USA
MBio 2:e00332-10. 2011..We conclude that SLO interferes with the internalization of GAS through local perturbation of the keratinocyte cell membrane and disruption of a clathrin-dependent uptake pathway...
- Identification of a novel pneumococcal vaccine antigen preferentially expressed during meningitis in miceLayla K Mahdi
Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia
J Clin Invest 122:2208-20. 2012..Our results provide the basis of a strategy that can be adapted to identify genes that contribute to the development of meningitis caused by other pathogens...
- Protein kinase C-α and arginase I mediate pneumolysin-induced pulmonary endothelial hyperpermeabilityRudolf Lucas
Vascular Biology Center and Dept of Pharmacology and Toxicology, Georgia Health Sciences University, 1459 Laney Walker Blvd, Augusta, GA 30912 2500, USA
Am J Respir Cell Mol Biol 47:445-53. 2012..These results identify PKC-α and arginase I as potential upstream and downstream therapeutic targets in PLY-induced pulmonary endothelial dysfunction...
- Extracellular group A Streptococcus induces keratinocyte apoptosis by dysregulating calcium signallingColette Cywes Bentley
Channing Laboratory, Brigham and Women s Hospital, Boston, MA 02115, USA
Cell Microbiol 7:945-55. 2005..We propose that cell signalling initiated by extracellular GAS compromises the epithelial barrier by inducing premature keratinocyte differentiation and apoptosis, thereby facilitating GAS invasion of deeper tissues...
- Export requirements of pneumolysin in Streptococcus pneumoniaeKatherine E Price
Tufts University School of Medicine and Howard Hughes Medical Institute, Department of Molecular Biology and Microbiology, Boston, Massachusetts, USA
J Bacteriol 194:3651-60. 2012..We show that B. subtilis can export Ply, suggesting that the export pathway is conserved. Finally, through truncation and domain swapping analyses, we show that export is dependent on domain 2 of Ply...
- Immunization with a combination of three pneumococcal proteins confers additive and broad protection against Streptococcus pneumoniae Infections in MiceKaifeng Wu
Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine Ministry of Education, Chongqing Medical University, Chongqing 400016, People s Republic of China
Infect Immun 78:1276-83. 2010..These results indicate that system immunization with the combination of pneumococcal antigens could provide an additive and broad protection against Streptococcus pneumoniae in pneumonia and sepsis infection models...
- Pneumolysin with low hemolytic activity confers an early growth advantage to Streptococcus pneumoniae in the bloodRichard M Harvey
Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide 5005, Australia
Infect Immun 79:4122-30. 2011..In summary, we show that low-hemolytic-activity Ply alleles contribute to systemic virulence and may provide a survival advantage in the blood. Moreover, pneumococci expressing such alleles remain vulnerable to Ply-based vaccines...
- Tumor suppressor CYLD regulates acute lung injury in lethal Streptococcus pneumoniae infectionsJae Hyang Lim
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
Immunity 27:349-60. 2007..Thus, CYLD is detrimental for host survival, thereby indicating a mechanism underlying the high early mortality of pneumococcal pneumonia...
- A protein-based pneumococcal vaccine protects rhesus macaques from pneumonia after experimental infection with Streptococcus pneumoniaePhilippe DeNoel
GlaxoSmithKline Biologicals, Rixensart, Belgium
Vaccine 29:5495-501. 2011..In conclusion, AS02-adjuvanted PhtD-dPly vaccine protects against S. pneumoniae-induced pneumonia. It is probable that the protection is at least partially mediated by PhtD- and Ply-specific antibodies...
- Streptococcus pneumoniae-induced inhibition of rat ependymal cilia is attenuated by antipneumolysin antibodyRobert A Hirst
Department of Infection, Immunity and Inflammation, University of Leicester, LE2 7LX, UK
Infect Immun 72:6694-8. 2004..These data show that antibiotic lysis of Streptococcus pneumoniae can be detrimental to the ciliated ependyma and that antipneumolysin antibody may have a therapeutic potential...
- Identification of invasive serotype 1 pneumococcal isolates that express nonhemolytic pneumolysinLea Ann S Kirkham
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland
J Clin Microbiol 44:151-9. 2006..Pneumolysin is generally conserved throughout all serotypes of S. pneumoniae and is essential for full invasive disease; however, it appears that serotype 1 ST306 does not require hemolytically active pneumolysin to cause IPD...
- Cytotoxic effects of streptolysin o and streptolysin s enhance the virulence of poorly encapsulated group a streptococciGabriele Sierig
Channing Laboratory and Division of Infectious Diseases, Brigham and Women s Hospital, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
Infect Immun 71:446-55. 2003..Compared to SLO, SLS in this strain background has a more modest influence on GAS pathogenicity and the effect does not appear to involve bacterial resistance to phagocytosis...
- Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-OI Walev
Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University, 55101 Mainz, Germany
Proc Natl Acad Sci U S A 98:3185-90. 2001..botulinum C2-toxin resulted in enhanced secretion in cells. The presented method for introducing proteins into living cells should find multifaceted application in cell biology...
- Novel mucosal vaccines generated by genetic conjugation of heterologous proteins to pneumolysin (PLY) from Streptococcus pneumoniaeGill Douce
Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, UK
Vaccine 28:3231-7. 2010....
- Proinflammatory interactions of pneumolysin with human neutrophilsR Cockeran
Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, Institute for Pathology, University of Pretoria, Pretoria 0001, South Africa
J Infect Dis 183:604-11. 2001..Pneumolysin potentiates the proinflammatory activities of neutrophils by a pore-forming mechanism resulting in Ca2+ influx...
- Immunizations with pneumococcal surface protein A and pneumolysin are protective against pneumonia in a murine model of pulmonary infection with Streptococcus pneumoniaeDavid E Briles
Department of Microbiology, University of Alabama at Birmingham, USA
J Infect Dis 188:339-48. 2003....
- Contributions of pneumolysin, pneumococcal surface protein A (PspA), and PspC to pathogenicity of Streptococcus pneumoniae D39 in a mouse modelAbiodun D Ogunniyi
School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia 5005, Australia
Infect Immun 75:1843-51. 2007..These findings further demonstrate that the mechanism of pneumococcal pathogenesis is highly complex and multifactorial but ascribes a role for each of these virulence proteins, alone or in combination, in the process...
- THP-1 monocytes up-regulate intercellular adhesion molecule 1 in response to pneumolysin from Streptococcus pneumoniaeJustin Thornton
Department of Microbiology, University of Mississippi Medical Center, Jackson, 39216, USA
Infect Immun 73:6493-8. 2005..These data indicate that PLY induces transcription and production of a cell adhesion molecule involved in the inflammatory response that may play a role in pneumococcal infection...
- The autolytic enzyme LytA of Streptococcus pneumoniae is not responsible for releasing pneumolysinP Balachandran
Department of Microbiology, The University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Bacteriol 183:3108-16. 2001..Finally, under some infection conditions, mutations in pneumolysin and autolysin had different effects on virulence...
- Tumor suppressor cylindromatosis acts as a negative regulator for Streptococcus pneumoniae-induced NFAT signalingTomoaki Koga
Department of Microbiology and Immunology and Cardiovascular Research Institute, University of Rochester Medical Center, Rochester, New York 14642, USA
J Biol Chem 283:12546-54. 2008..pneumoniae infections through the NFAT-dependent mechanism and further identify CYLD as a negative regulator for NFAT signaling, thereby opening up new therapeutic targets for these diseases...
- Streptococcus pyogenes cytolysin-mediated translocation does not require pore formation by streptolysin ON Goundo Magassa
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
EMBO Rep 11:400-5. 2010..Moreover, despite the ability of mutants to translocate SPN, their cytotoxic effect requires SLO pore formation...
- Streptococcus pneumoniae deficient in pneumolysin or autolysin has reduced virulence in meningitisRobert A Hirst
Department of Infection, Immunity, and Inflammation, University of Leicester, Leicester, United Kingdom
J Infect Dis 197:744-51. 2008..The role played by pneumolysin and autolysin in pneumococcal meningitis is poorly understood...
- Decreased virulence of a pneumolysin-deficient strain of Streptococcus pneumoniae in murine meningitisAndreas Wellmer
Department of Neurology, University of Gottingen, Gottingen, Germany
Infect Immun 70:6504-8. 2002..Cerebellar and spleen bacterial titers, meningeal inflammation, and neuronal damage scores remained uninfluenced by the lack of any of the virulence factors...
- Cholesterol as treatment for pneumococcal keratitis: cholesterol-specific inhibition of pneumolysin in the corneaMary E Marquart
Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
Invest Ophthalmol Vis Sci 48:2661-6. 2007..The purpose of this study was to determine whether cholesterol, the host cell receptor for pneumolysin of Streptococcus pneumoniae, could effectively treat pneumococcal keratitis...
- Comparison of the effects of macrolides, amoxicillin, ceftriaxone, doxycycline, tobramycin and fluoroquinolones, on the production of pneumolysin by Streptococcus pneumoniae in vitroR Anderson
Medical Research Council MRC Unit for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria, and Tshwane Academic Division of the National Health Laboratory Service, South Africa
J Antimicrob Chemother 60:1155-8. 2007....
- Pneumolysin, PspA, and PspC contribute to pneumococcal evasion of early innate immune responses during bacteremia in miceLisa R Quin
Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA
Infect Immun 75:2067-70. 2007..Finally, blood clearance assays demonstrated that there was enhanced clearance of Deltaply PspA(-) PspC(-) pneumococci compared to the clearance of nonencapsulated pneumococci...
- Direct transmembrane interaction between actin and the pore-competent, cholesterol-dependent cytolysin pneumolysinSabrina Hupp
DFG Membrane Cytoskeleton Interaction Group, Institute of Pharmacology and Toxicology and Rudolf Virchow Centre for Experimental Biomedical Science, University of Wurzburg, Versbacherstr 9, 97078 Wurzburg, Germany
J Mol Biol 425:636-46. 2013..Our results demonstrate the first example of a direct interaction of a pore-forming toxin with cytoskeletal components, suggesting that the cross talk between pore-forming cytolysins and cells is more complex than previously thought...
- Combined contributions of streptolysin O and streptolysin S to virulence of serotype M5 Streptococcus pyogenes strain ManfredoMichael C Fontaine
School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
Infect Immun 71:3857-65. 2003....
- Virulence factors and the pathogenesis of disease caused by Streptococcus pneumoniaeT J Mitchell
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK
Res Microbiol 151:413-9. 2000..The use of molecular approaches to identifying and studying putative virulence factors in combination with the application of animal models has allowed some of the mechanisms of the disease process to be defined...
- Persistence of pneumolysin in the cerebrospinal fluid of patients with pneumococcal meningitis is associated with mortalityEmma C Wall
Clinical Research Group, Liverpool School of Tropical Medicine, United Kingdom
Clin Infect Dis 54:701-5. 2012..In patient samples we showed that pneumolysin levels in CSF remained high after 48 hours in nonsurvivors of meningitis compared with survivors. Selective antipneumolysin treatment may present a novel therapeutic option...
- Astrocytic tissue remodeling by the meningitis neurotoxin pneumolysin facilitates pathogen tissue penetration and produces interstitial brain edemaSabrina Hupp
DFG Membrane Cytoskeleton Interaction Group, Institute of Pharmacology and Toxicology and Rudolf Virchow Center for Experimental Medicine, University of Wurzburg, Versbacherstr 9, 97078 Wurzburg, Germany
Glia 60:137-46. 2012..We suggest that PLY can increase the permeability of brain tissue toward pathogenic factors and bacteria in the course of meningitis, thus contributing to the deterioration caused by the disease...
- Changes in astrocyte shape induced by sublytic concentrations of the cholesterol-dependent cytolysin pneumolysin still require pore-forming capacityChristina Förtsch
DFG Membrane, Cytoskeleton Interaction Group, Institute of Pharmacology and Toxicology and Rudolf Virchow Center for Experimental Medicine, University of Wurzburg, Versbacherstr 9, 97078 Wurzburg, Germany
Toxins (Basel) 3:43-62. 2011....
- Nitric oxide levels regulate macrophage commitment to apoptosis or necrosis during pneumococcal infectionHelen M Marriott
Division of Genomic Medicine, University of Sheffield School of Medicine and Biomedical Sciences, Sheffield, UK
FASEB J 18:1126-8. 2004..These results illustrate important roles for NO in the integration of host defense and regulation of inflammation in human macrophages...
- Specific behavior of intracellular Streptococcus pyogenes that has undergone autophagic degradation is associated with bacterial streptolysin O and host small G proteins Rab5 and Rab7Atsuo Sakurai
Department of Pediatric Dentistry, Tokyo Dental College, 1 2 2 Masago, Mihama ku, Chiba 261 8502, Japan
J Biol Chem 285:22666-75. 2010..This intracellular behavior of GAS is unique and distinct from that of other types of bacterial invaders. Our results provide a new picture of GAS infection and host-cell responses in epithelial cells...
- The molecular mechanism of pneumolysin, a virulence factor from Streptococcus pneumoniaeJ Rossjohn
The Ian Potter Foundation Protein Crystallography Laboratory, St Vincent s Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria, 3065, Australia
J Mol Biol 284:449-61. 1998....
- Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2A Yoshimura
Maxwell Finland Laboratory for Infectious Diseases, Boston University School of Medicine, Boston Medical Center, MA 02118, USA
J Immunol 163:1-5. 1999..Soluble peptidoglycan substituted for whole organisms. These data suggest that the similarity of clinical response to invasive infection by Gram-positive and Gram-negative bacteria is due to bacterial recognition via similar TLRs...
- Neurotoxicity of pneumolysin, a major pneumococcal virulence factor, involves calcium influx and depends on activation of p38 mitogen-activated protein kinaseArgyrios K Stringaris
Department of Neurology, University of Gottingen, 37075, Gottingen, Germany
Neurobiol Dis 11:355-68. 2002..Modulation of cell death pathways activated by pneumolysin may influence the outcome of pneumococcal meningitis...
- Cytolysin-dependent evasion of lysosomal killingAnders Hakansson
Channing Laboratory, Brigham and Women s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 102:5192-7. 2005....
- Dose-dependent activation of microglial cells by Toll-like receptor agonists alone and in combinationSandra Ebert
Department of Neurology, University of Gottingen, Robert Koch Str 40, 37075 Gottingen, Germany
J Neuroimmunol 159:87-96. 2005..Costimulation with submaximum concentrations was additive or supraadditive, suggesting that even low concentrations of products of infectious agents can lead to microglial activation via TLRs...
- Pneumolysin potentiates production of prostaglandin E(2) and leukotriene B(4) by human neutrophilsR Cockeran
Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, Institute for Pathology, University of Pretoria, Pretoria, South Africa
Infect Immun 69:3494-6. 2001..These interactions of pneumolysin with neutrophils may result in dysregulation of inflammatory responses during pneumococcal infection...
- The effects of pneumolysin and hydrogen peroxide, alone and in combination, on human ciliated epithelium in vitroC Feldman
Division of Pulmonology, Department of Medicine, Johannesburg Hospital, University of the Witwatersrand, South Africa
Respir Med 96:580-5. 2002..These actions of pneumolysin and H2O2 may contribute to the pathogenesis of respiratory tract infections caused by the pneumococcus...
- Incompetence of neutrophils to invasive group A streptococcus is attributed to induction of plural virulence factors by dysfunction of a regulatorManabu Ato
Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan
PLoS ONE 3:e3455. 2008..These results suggest that the abilities of GAS to abrogate PMN functions can determine the onset and severity of invasive GAS infection...
- Functional analysis of pneumolysin by use of monoclonal antibodiesJ R de los Toyos
Facultad de Medicina, Universidad de Oviedo, Spain
Infect Immun 64:480-4. 1996..This site is engaged in the oligomerization process involved in the formation of pores in cell membranes. Two of these epitopes are also present in the related toxin perfringolysin O...
- Differential role of p38 mitogen activated protein kinase for cellular recovery from attack by pore-forming S. aureus alpha-toxin or streptolysin OMatthias Husmann
Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University, Mainz, Hochhaus am Augustusplatz, 55131 Mainz, Germany
Biochem Biophys Res Commun 344:1128-34. 2006..In contrast, blockade of p38 in SLO permeabilized cells did not affect the capacity of the cells to replenish their ATP stores...
- Human lung mast cells mediate pneumococcal cell death in response to activation by pneumolysinGlenn Cruse
Department of Infection, Immunity and Inflammation, Institute for Lung Health, University of Leicester Medical School, Leicester, United Kingdom
J Immunol 184:7108-15. 2010..This suggests that mast cells provide an early warning system and potentially limit pneumococcal dissemination early in the course of invasive pulmonary pneumococcal disease...
- Pneumococcal carriage and otitis media induce salivary antibodies to pneumococcal surface adhesin a, pneumolysin, and pneumococcal surface protein a in childrenB Simell
Department of Vaccines, National Public Health Institute, Helsinki, Finland
J Infect Dis 183:887-96. 2001..The findings indicate that pneumococcal carriage and AOM induce local production of anti-PsaA, anti-Ply, and anti-PspA antibodies early in life...
- Pneumolysin, a protein toxin of Streptococcus pneumoniae, induces nitric oxide production from macrophagesJ S Braun
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Infect Immun 67:3750-6. 1999..The properties of Pln, previously identified as a pore-forming hemolysin, also include a role as a general inflammatory agonist...
- Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCRC E Corless
Meningococcal Reference Unit, Manchester Public Health Laboratory, Withington Hospital, Manchester M20 2LR, United Kingdom
J Clin Microbiol 39:1553-8. 2001..The ctrA primer set used in the multiplex PCR was found to be more sensitive (P < 0.0001) than the ctrA primers that had been used for meningococcal PCR testing at that time...
- Pneumolysin is the main inducer of cytotoxicity to brain microvascular endothelial cells caused by Streptococcus pneumoniaeG Zysk
Department of Medical Microbiology and Virology, University of Duesseldorf, Duesseldorf, Germany
Infect Immun 69:845-52. 2001..The results show that pneumolysin is an important component for damaging the BBB and may contribute to the entry of pneumococci into the cerebral compartment and to the development of brain edema in pneumococcal meningitis...
- Reduced release of pneumolysin by Streptococcus pneumoniae in vitro and in vivo after treatment with nonbacteriolytic antibiotics in comparison to ceftriaxoneAnnette Spreer
Department of Neurology, University of Gottingen, Gottingen, Germany
Antimicrob Agents Chemother 47:2649-54. 2003....
- Intracellular Ca(2+) operates a switch between repair and lysis of streptolysin O-perforated cellsE B Babiychuk
Department of Cell Biology, Institute of Anatomy, University of Bern, Bern, Switzerland
Cell Death Differ 16:1126-34. 2009..These findings show that the extent of [Ca(2+)](i) elevation determines the fate of targeted cells and establishes how different Ca(2+)-dependent mechanisms facilitate either cell survival or death...
- The streptococcal exotoxin streptolysin O activates mast cells to produce tumor necrosis factor alpha by p38 mitogen-activated protein kinase- and protein kinase C-dependent pathwaysMichael Stassen
Institute of Immunology Institute of Medical Microbiology, Johannes Gutenberg University, Mainz, Germany
Infect Immun 71:6171-7. 2003..However, hyperstimulation of mast cells might also lead to overproduction of TNF-alpha, which would then promote the development of toxic streptococcal syndromes...
- Enhancement of streptolysin O activity and intrinsic cytotoxic effects of the group A streptococcal toxin, NAD-glycohydrolaseAthanasios Michos
Division of Infectious Diseases, Children s Hospital Boston, Massachusetts 02215, USA
J Biol Chem 281:8216-23. 2006..Together, the results have provided mechanistic insight into the cytotoxic effects of a unique bipartite bacterial toxin...
- Pneumococcal behavior and host responses during bronchopneumonia are affected differently by the cytolytic and complement-activating activities of pneumolysinRania Jounblat
Department of Microbiology and Immunology, University of Leicester, University Road, Leicester, LE1 9HN, UK
Infect Immun 71:1813-9. 2003..The complement-activating activity of pneumolysin affected the accumulation of T cells, whereas the toxin's cytolytic activity influenced neutrophil recruitment into lung tissue...
- Nucleotide sequence of the gene for perfringolysin O (theta-toxin) from Clostridium perfringens: significant homology with the genes for streptolysin O and pneumolysinR K Tweten
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190
Infect Immun 56:3235-40. 1988..The location of these 12 residues was conserved in all three toxins when the primary sequences were aligned for maximum homology...
- Immunization with pneumolysin protects against both retinal and global damage caused by Streptococcus pneumoniae endophthalmitisMelissa E Sanders
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA
J Ocul Pharmacol Ther 26:571-7. 2010..To determine whether immunization with pneumolysin (PLY) protects against pneumococcal endophthalmitis...
- Induction of necrosis and apoptosis of neutrophil granulocytes by Streptococcus pneumoniaeG Zysk
Institute of Medical Microbiology and Virology, University of Dusseldorf, Dusseldorf, Germany
Clin Exp Immunol 122:61-6. 2000..These results demonstrated that pneumococci induce PMN cell death. Depending on the intensity of the stimulus, PMN necrosis and apoptosis were observed...
- Ultrastructural analysis of the membrane insertion of domain 3 of streptolysin OKachiko Sekiya
Laboratory of Electron Microscopy, School of Pharmacy, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo 108 8641, Japan
Microbes Infect 9:1341-50. 2007..On the basis of a model of the oligomeric structure of SLO, we propose some new details of the mechanism of hemolysis by SLO...
- Discovery of a widely distributed toxin biosynthetic gene clusterShaun W Lee
Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 105:5879-84. 2008..Given our findings, it is likely that the discovery of similar peptidic toxins will rapidly expand to existing and emerging genomes...
- DNA vaccines based on genetically detoxified derivatives of pneumolysin fail to protect mice against challenge with Streptococcus pneumoniaeDaniela M Ferreira
Centro de Biotecnologia, Instituto Butantan, Sao Paulo, SP, Brazil
FEMS Immunol Med Microbiol 46:291-7. 2006..Immunization with DNA vaccines expressing pneumolysoids, on the other hand, induced a significantly lower antibody response and no protection was observed...
- Clinical use and interpretation of group A streptococcal antibody tests: a practical approach for the pediatrician or primary care physicianAnita Shet
Department of Pediatrics, World Health Organization Collaborating Center for Reference and Research on Streptococci, University of Minnesota Medical School, Minneapolis, USA
Pediatr Infect Dis J 21:420-6; quiz 427-30. 2002
- Pneumolysin-mediated activation of NFkappaB in human neutrophils is antagonized by docosahexaenoic acidH Fickl
Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, University of Pretoria, Pretoria
Clin Exp Immunol 140:274-81. 2005....
- Pneumococcal virulence factors: structure and functionM J Jedrzejas
Department of Microbiology, University of Alabama at Birmingham, 933 19th Street South, Birmingham, AL 35294
Microbiol Mol Biol Rev 65:187-207 ; first page, table of contents. 2001....
- Rapid microtubule bundling and stabilization by the Streptococcus pneumoniae neurotoxin pneumolysin in a cholesterol-dependent, non-lytic and Src-kinase dependent manner inhibits intracellular traffickingAsparouh I Iliev
Laboratory for Molecular and Cellular Systems, Department of Neuro and Sensory Physiology, Instutute for Physiology and Pathophysiology, University Medicine Gottingen, 37073 Gottingen, Germany
Mol Microbiol 71:461-77. 2009....
- Assembly and topography of the prepore complex in cholesterol-dependent cytolysinsAlejandro P Heuck
Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, Texas 77843 1114, USA
J Biol Chem 278:31218-25. 2003....
- Repair of injured plasma membrane by rapid Ca2+-dependent endocytosisVincent Idone
Section of Microbial Pathogenesis and 2Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
J Cell Biol 180:905-14. 2008..These findings provide an important new insight into how cells protect themselves not only from mechanical injury but also from microbial toxins and pore-forming proteins produced by the immune system...
- Tissue-specific contributions of pneumococcal virulence factors to pathogenesisCarlos J Orihuela
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
J Infect Dis 190:1661-9. 2004..We conclude that transitions between body sites require virulence determinants distinct from those involved in organ-specific replication...
- Prospective study to determine clinical relevance of detection of pneumococcal DNA in sera of children by PCRR Dagan
Pediatric Infectious Disease Unit, Soroka University Medical Center, Ben Gurion University of the Negev, Beer Sheva, Israel
J Clin Microbiol 36:669-73. 1998....
- The apoptotic response to pneumolysin is Toll-like receptor 4 dependent and protects against pneumococcal diseaseAmit Srivastava
Division of Infectious Diseases, Department of Medicine, Children s Hospital, Boston, MA 02115, USA
Infect Immun 73:6479-87. 2005..Overall, our results strongly suggest that protection against pneumococcal disease is dependent on the TLR4-mediated enhancement of pneumolysin-induced apoptosis...
- Streptococcus pneumoniae: the role of apoptosis in host defense and pathogenesisHelen M Marriott
Division of Genomic Medicine, F Floor, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK
Int J Biochem Cell Biol 38:1848-54. 2006..Apoptosis induction may be harmful or potentially beneficial during pneumococcal infection and understanding its function in each setting is essential to allow specific therapeutic intervention...
- Protection against Streptococcus pneumoniae elicited by immunization with pneumolysin and CbpAA D Ogunniyi
Department of Molecular Biosciences, Adelaide University, Adelaide, South Australia 5005, Australia
Infect Immun 69:5997-6003. 2001..Mice immunized with PdB in MPL plus AlPO4 were also significantly better protected than mice that received PdB in AlPO4 alone...
- Structural and functional dissection of the heterocyclic peptide cytotoxin streptolysin SDouglas A Mitchell
Department of Pharmacology, University of California San Diego, La Jolla, CA 92093, USA
J Biol Chem 284:13004-12. 2009..This new structural information has ramifications for future antimicrobial therapies...
- Pneumococci induced TLR- and Rac1-dependent NF-kappaB-recruitment to the IL-8 promoter in lung epithelial cellsBernd Schmeck
Department of Internal Medicine Infectious Diseases, Charite Universitatsmedizin Berlin, Berlin, Germany
Am J Physiol Lung Cell Mol Physiol 290:L730-L737. 2006..In summary, these results suggest that S. pneumoniae activate human epithelial cells by TLR1/2 and a phosphatidylinositol 3-kinase- and Rac1-dependent NF-kappaB-recruitment to the IL-8 promoter...
- Pneumolysin-induced complement depletion during experimental pneumococcal bacteremiaR B Alcantara
Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
Infect Immun 69:3569-75. 2001..These studies suggest that under limiting concentrations of complement, the expression of pneumolysin by pneumococci has a significant, negative effect on serum complement levels and reduces serum opsonic activity...
- A specific and ultrasensitive chemiluminescent sandwich ELISA test for the detection and quantitation of pneumolysinM D Cima-Cabal
, Facultad de Medicina, Universidad de Oviedo, Spain
J Immunoassay Immunochem 22:99-112. 2001..The test is highly specific as no other purified toxins or human pneumonia- or meningitis-associated bacteria yielded false-positive results. This specific and highly sensitive method could help in the diagnosis of human infections...
- Resealing of large transmembrane pores produced by streptolysin O in nucleated cells is accompanied by NF-kappaB activation and downstream eventsIwan Walev
Institute of Medical Microbiology, Johannes Gutenberg University Mainz, D 55101 Mainz, Germany
FASEB J 16:237-9. 2002..The study provides definitive evidence that a transient transmembrane pore suffices to trigger productive transcriptional activation in a target cell...
- MKP1 regulates the induction of MUC5AC mucin by Streptococcus pneumoniae pneumolysin by inhibiting the PAK4-JNK signaling pathwayUn Hwan Ha
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642, USA
J Biol Chem 283:30624-31. 2008..These studies provide novel insights into the molecular mechanisms underlying the tight regulation of mucin overproduction in the pathogenesis of airway infectious diseases and may lead to development of new therapeutic strategies...
- Natural development of antibodies to pneumococcal surface protein A, pneumococcal surface adhesin A, and pneumolysin in relation to pneumococcal carriage and acute otitis mediaS Rapola
National Public Health Institute, Dept of Vaccines, 00300 Helsinki, Finland
J Infect Dis 182:1146-52. 2000..All age groups were capable of producing antibodies to the 3 proteins. The antibody concentrations increased with age and were strongly associated with pneumococcal exposure, whether by carriage or infection (acute otitis media)...
- Bacterial programmed cell death of cerebral endothelial cells involves dual death pathwaysDaniela Bermpohl
Department of Neurology, Center of Anatomy, Charite Universitatsmedizin Berlin, Berlin, Germany
J Clin Invest 115:1607-15. 2005..These findings broaden the scope of bacterial-induced PCD, link these effects to innate immune TLRs, and provide insight into the acute and persistent phases of damage during meningitis...
- Host and bacterial factors contributing to the clearance of colonization by Streptococcus pneumoniae in a murine modelAnnemarie M C van Rossum
University of Pennsylvania, 402A Johnson Pavilion, Philadelphia, PA 19104 6076, USA
Infect Immun 73:7718-26. 2005..We conclude that both innate and CD4+ T-cell-mediated immunity and proinflammatory bacterial factors, rather than a humoral adaptive immune response, are important for clearance of S. pneumoniae from the murine nasopharynx...
- Dual function of pneumolysin in the early pathogenesis of murine pneumococcal pneumoniaJ B Rubins
Pulmonary Disease Division, Veterans Affairs Medical Center, Minneapolis, Minnesota
J Clin Invest 95:142-50. 1995..Moreover, both the cytotoxic and the complement-activating activities of pneumolysin may contribute independently to the acute pulmonary injury and the high rates of bacteremia which characterize pneumococcal pneumonia...
- A common denominator of pathogenesis;a rare opportunity for novel therapeutic deDOUGLAS ALAN MITCHELL; Fiscal Year: 2011..A greater understanding of these processes will lay the foundation for developing the next generation of antimicrobial drugs. ..
- Cardiac dysfunction in StrepTSS: interplay of SLO and MMPsDennis L Stevens; Fiscal Year: 2013....
- Single RNA sensitive probes for studying viral replication and buddingPhilip J Santangelo; Fiscal Year: 2013....
- Host-pathogen signaling in group A streptococcal infectionMichael R Wessels; Fiscal Year: 2012..Understanding this process may lead to new strategies for prevention or treatment of GAS infection. ..
- CYTOLYSIN-MEDIATED TRANSLOCATION IN S. PYOGENES VIRULENCMichael G Caparon; Fiscal Year: 2013..This information will be important for understanding how the multiple toxins of S. pyogenes interact with each other and with host cells to unravel how this pathogen can cause such a diverse range of different diseases. ..
- Pore Formation by Cholesterol Dependent CytolysinsRodney K Tweten; Fiscal Year: 2013....
- Vaccine and therapeutic antibodies against Group A StreptococcusBenfang Lei; Fiscal Year: 2013..The project has the potential to lead to the development of a broad vaccine against GAS diseases and an antibody therapy to treat severe GAS infections. ..
- Design and Use of Novel BacteriocinsShaun Lee; Fiscal Year: 2011..This proposal presents a method of generating an entirely new class of antibiotic compounds, many of which will undoubtedly have tremendous public health value. ..
- Evasion of Innate Immunity by Group A StreptococcusBenfang Lei; Fiscal Year: 2013..Overall, this project has the potential to define a novel mechanism for evasion of innate immunity by GAS and to establish a paradigm for bacterial inhibition of neutrophil recruitment and function. ..
- HETEROTRIMERIC G PROTEINS AND EXOCYTOSISBHANU JENA; Fiscal Year: 1999..Understanding the role of the secretory granule membrane-associated G-proteins in exocytosis will help us understand better this fundamental cellular process which when impaired leads to a number of diseases. ..
- FUNCTION OF A BRAIN CALCIUM-BINDING PROTEINDONALD WOLFF; Fiscal Year: 1991..We propose to purify this phosphatase by immunoaffinity chromatography and to compare its physical, kinetic and chemical properties with the bovine brain homolog...
- REGULATION OF EXOCYTOSIS IN MAST CELLSJ Castle; Fiscal Year: 2004..abstract_text> ..
- MUCOID VARIANTS OF S PYOGENES EXHIBIT ENHANCED VIRULENCEN Engleberg; Fiscal Year: 2001..If so, this observation will have major impact on the future analyses of human cases. ..
- STREPTOCOCCUS PYOGENES--CLONAL ANALYSIS OF VIRULENCEEdward Graviss; Fiscal Year: 2000..The P.I. also proposes to explore in depth his own finding, recently published, that SpeB cleaves and activates a matrix metallo-protease produced by human endothelial cells. ..
- P56C2, A NOVEL C2 DOMAIN PROTEIN OF LEUKOCYTESBERNARD BABIOR; Fiscal Year: 2003..abstract_text> ..
- CONTROL OF AUTOLYSIS IN PNEUMOCOCCIElaine Tuomanen; Fiscal Year: 2006..abstract_text> ..
- SLS: Molecular Basis and Role in Invasive GAS DiseaseVictor Nizet; Fiscal Year: 2006..These hypotheses will be tested by molecular genetic studies, attempts protein purification and antibody development, and the use of targeted SLS mutants in in vitro assays of phagocytic function and our in vivo mouse model of GAS NF. ..
- Sialic Acid O-Acetylation in GBS Pathogenesis & ImmunityVictor Nizet; Fiscal Year: 2010..Companion analyses will be performed on GBS isolates and human sera from recent prospective seroepidemiologic studies. ..
- MOLECULAR AND CELLULAR BACTERIAL PATHOGENESISDavid Briles; Fiscal Year: 2008..The MCBP advisory committee will advise the trainees in their research and training programs and in their search for a job or postdoctoral position at the completion of their MCBP training. [unreadable] [unreadable]..
- DEFENSINS--ANTIMICROBIAL PEPTIDES OF HUMAN NEUTROPHILSRobert Lehrer; Fiscal Year: 2002..The work with murine neutrophils, whose content of endogenous antimicrobial polypeptides differs greatly from those of humans, will provide essential information relevant to the interpretation of murine models of human infection. ..
- Chemical Genetic Analysis of Platelet Granule SecretionRobert Flaumenhaft; Fiscal Year: 2008..Once identified, compounds will be tested in a previously established battery of platelet function tests and evaluated for antithrombotic potential in an in vivo murine model of thrombus formation. [unreadable] [unreadable] [unreadable]..
- ALTRUINS, NATURAL ANTIBIOTICS OF THE MALE GENITAL TRACTRobert Lehrer; Fiscal Year: 2001..S. are sexually transmitted. According to CDC estimates, STDs other than AIDS add 10 billion dollars to the nation's annual health care I costs. The proposed studies could lead to novel approaches for STD prophylaxis. ..
- MACROMOLECULAR ARRAYS IN EXTRACELLULAR MATRIX OF COCHLEAVladimir Tsuprun; Fiscal Year: 2003..Methods of structural crystallography, Fourier (Fast Fourier Transformation) image analysis of periodic objects, and computer averaging to obtain enhanced, noise-free images will be applied in this study. ..
- Retrocyclins: Cyclic mini-defensins that inactivate anthrax toxinsROBERT IRVING contact LEHRER; Fiscal Year: 2010..anthracis spores in mouse models as per the revised Specific Aims. All remaining Aims can be addressed in a two-year time period. PHS ..
- STRUCTURAL STUDIES OF BACTERIAL HYALURONATE LYASE ENZYMEMARK JEDRZEJAS; Fiscal Year: 2003..The results will enhance our knowledge about mechanisms involved in the action of the enzyme and in the pathogenesis of S. pneumoniae bacteria. ..
- STRUCTURE/FUNCTIONS OF HUMAN TEAR LIPOPHILINRobert Lehrer; Fiscal Year: 2001..The long-term objectives for this research are to learn how lipophilin contributes to corneal and conjuctival health, and to explore its potential for creating new diagnostic and therapeutic modalities. ..
- MHC CLASS I ANTIGEN PROCESSING AND PRESENTATIONMatthew Androlewicz; Fiscal Year: 2001..abstract_text> ..
- SNARE HYPOTHESIS AT NERVE ENDINGSEDWARD STUENKEL; Fiscal Year: 2002..In summary, an understanding of the mechanisms by which nSec1p regulates SNARE protein interactions may be essential to full understanding of both short and long-term processes of synaptic plasticity and memory. ..
- NEUTROPHIL RECRUITMENT IN LPS-INDUCED AIRWAY DISEASEJessica Moreland; Fiscal Year: 2004....
- Biacore 3000 SPR InstrumentRobert Lehrer; Fiscal Year: 2004..These include the development of new agents and approaches to prevent and treat infections caused by HIV-1 and other viral pathogens, and fundamental issues related to cell targeting and survival. ..
- Polymicrobial induction of epithelial inflammationAdam Ratner; Fiscal Year: 2008..Ratner to a wide variety of techniques in the areas of cell biology, biochemistry, physiology, and molecular biology, thus providing a solid foundation for beginning an independent research career. [unreadable] [unreadable] [unreadable]..
- AIRWAY SMOOTH MUSCLE IN HEALTH AND DISEASERONALD COBURN; Fiscal Year: 2004..In particular, all of these diseases cause physiological alterations that influence the length at which smooth muscle cells contract and relax. ..
- Role of Racl in Regulated ExocytosisEDWARD STUENKEL; Fiscal Year: 2004..The work proposed attempts to provide a greater understanding of cytosolic and membrane delimited signaling pathways that exert an important regulatory influence on Ca2+- dependent secretory responsiveness. ..
- Air Quality Improvements of The Animal FacilityMichael Gilmore; Fiscal Year: 2005..This will significantly improve the quality of vision research conducted by the Faculty at the Schepens. ..
- Resistant Enterococci in the GI tract ConsortiumMichael Gilmore; Fiscal Year: 2004..Based on these results, well-grounded specific questions can be postulated and tested subsequently to verify their importance in vivo, ultimately leading to the design of agents to prevent colonization by antibiotic resistant strains. ..
- ORAL BIOFILMS: DIFFERENTIAL DISPLAY AND GENETIC EXCHANGEMichael Gilmore; Fiscal Year: 2003..abstract_text> ..
- ALPHA HELICAL CATHELICIDINS AND HOST DEFENSERobert Lehrer; Fiscal Year: 2003..The experiments will expand our knowledge about these important peptides, and should provide the information needed to develop them as antibiotics for topical bronchopulmonary use in cystic fibrosis. ..
- Drosophila model of antibiotic resistant infectionMichael Gilmore; Fiscal Year: 2006..abstract_text> ..
- SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETIONRobert Flaumenhaft; Fiscal Year: 2007..abstract_text> ..
- Theta-defensins Novel HIV-1 Uptake InhibitorsRobert Lehrer; Fiscal Year: 2007..Specific Aim 2 will be realized by a combination of microchemical techniques, including surface plasmon resonance, tandem mass spectrometry, and state-of-the-art lectinomic and peptidomic chemistry. ..
- Retrocyclin reinforcement of pulmonary defenses against viral aerosolsRobert Lehrer; Fiscal Year: 2007..unreadable] [unreadable]..
- Role of CIC-3 channels in regulation of cell volume and shape in neutrophilsJessica Moreland; Fiscal Year: 2007..The studies proposed will advance our fundamental knowledge of the function of neutrophils in the maintenance of normal immune system function. [unreadable] [unreadable] [unreadable]..
- Regulation of tomosyn-SNARE complex assembly in neurosecretionEdward L Stuenkel; Fiscal Year: 2010....