viral antigens

Summary

Summary: Substances elaborated by viruses that have antigenic activity.

Top Publications

  1. pmc Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans
    Rebecca J Garten
    WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Science 325:197-201. 2009
  2. ncbi Epidermal viral immunity induced by CD8alpha+ dendritic cells but not by Langerhans cells
    Rhys S Allan
    Department of Microbiology and Immunology, The University of Melbourne, Melbourne, Victoria 3010, Australia
    Science 301:1925-8. 2003
  3. ncbi The nucleosomal surface as a docking station for Kaposi's sarcoma herpesvirus LANA
    Andrew J Barbera
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 311:856-61. 2006
  4. pmc Immunoproteasomes shape immunodominance hierarchies of antiviral CD8(+) T cells at the levels of T cell repertoire and presentation of viral antigens
    W Chen
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 193:1319-26. 2001
  5. pmc Epitope mapping of the hemagglutinin molecule of a highly pathogenic H5N1 influenza virus by using monoclonal antibodies
    Nikolai V Kaverin
    D I Ivanovsky Institute of Virology, 123098 Moscow, Russia
    J Virol 81:12911-7. 2007
  6. ncbi Epochal evolution shapes the phylodynamics of interpandemic influenza A (H3N2) in humans
    Katia Koelle
    Department of Ecology and Evolutionary Biology, 2019 Kraus Natural Science Building, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109 1048, USA
    Science 314:1898-903. 2006
  7. ncbi Kaposi's sarcoma-associated herpesvirus LANA hitches a ride on the chromosome
    Andrew J Barbera
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Cell Cycle 5:1048-52. 2006
  8. ncbi Characterization of an antigenic site that contains a dominant, type-specific neutralization determinant on the envelope protein domain III (ED3) of dengue 2 virus
    Gregory D Gromowski
    Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, and Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    Virology 366:349-60. 2007
  9. pmc Differential antigen presentation regulates the changing patterns of CD8+ T cell immunodominance in primary and secondary influenza virus infections
    Sherry R Crowe
    Trudeau Institute, P O Box 59, Saranac Lake, NY 12983, USA
    J Exp Med 198:399-410. 2003
  10. ncbi Noncytolytic control of viral infections by the innate and adaptive immune response
    L G Guidotti
    Department of Molecular and Experimental Medicine, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Annu Rev Immunol 19:65-91. 2001

Detail Information

Publications293 found, 100 shown here

  1. pmc Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans
    Rebecca J Garten
    WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Science 325:197-201. 2009
    ..Antigenically the viruses are homogeneous and similar to North American swine A(H1N1) viruses but distinct from seasonal human A(H1N1)...
  2. ncbi Epidermal viral immunity induced by CD8alpha+ dendritic cells but not by Langerhans cells
    Rhys S Allan
    Department of Microbiology and Immunology, The University of Melbourne, Melbourne, Victoria 3010, Australia
    Science 301:1925-8. 2003
    ..Thus, the traditional view of Langerhans cells in epidermal immunity needs to be revisited to accommodate a requirement for other dendritic cells in this response...
  3. ncbi The nucleosomal surface as a docking station for Kaposi's sarcoma herpesvirus LANA
    Andrew J Barbera
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 311:856-61. 2006
    ..Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency...
  4. pmc Immunoproteasomes shape immunodominance hierarchies of antiviral CD8(+) T cells at the levels of T cell repertoire and presentation of viral antigens
    W Chen
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 193:1319-26. 2001
    ..These findings indicate that in addition to their effects on the presentation of foreign antigens, immunoproteasomes influence T(CD8+) responses by modifying the repertoire of responding T(CD8+)...
  5. pmc Epitope mapping of the hemagglutinin molecule of a highly pathogenic H5N1 influenza virus by using monoclonal antibodies
    Nikolai V Kaverin
    D I Ivanovsky Institute of Virology, 123098 Moscow, Russia
    J Virol 81:12911-7. 2007
    ....
  6. ncbi Epochal evolution shapes the phylodynamics of interpandemic influenza A (H3N2) in humans
    Katia Koelle
    Department of Ecology and Evolutionary Biology, 2019 Kraus Natural Science Building, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109 1048, USA
    Science 314:1898-903. 2006
    ..It captures the observed boom-and-bust pattern of viral evolution, with periods of antigenic stasis during which genetic diversity grows, and with episodic contraction of this diversity during cluster transitions...
  7. ncbi Kaposi's sarcoma-associated herpesvirus LANA hitches a ride on the chromosome
    Andrew J Barbera
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Cell Cycle 5:1048-52. 2006
    ..Our data offer further insight into the mechanism of KSHV latency, and also reveal a new concept for a role of the nucleosome as a docking site for other proteins...
  8. ncbi Characterization of an antigenic site that contains a dominant, type-specific neutralization determinant on the envelope protein domain III (ED3) of dengue 2 virus
    Gregory D Gromowski
    Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, and Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    Virology 366:349-60. 2007
    ..Overall, the conserved antigenic site recognized by all seven MAbs is likely to be a dominant DENV-2 type-specific, neutralization determinant...
  9. pmc Differential antigen presentation regulates the changing patterns of CD8+ T cell immunodominance in primary and secondary influenza virus infections
    Sherry R Crowe
    Trudeau Institute, P O Box 59, Saranac Lake, NY 12983, USA
    J Exp Med 198:399-410. 2003
    ..Thus, differential antigen presentation impacts both the specificity of the T cell response and the efficacy of peptide-based vaccination strategies...
  10. ncbi Noncytolytic control of viral infections by the innate and adaptive immune response
    L G Guidotti
    Department of Molecular and Experimental Medicine, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Annu Rev Immunol 19:65-91. 2001
    ..In keeping with these concepts, it is not surprising that a number of viruses encode proteins that have the potential to inhibit the antiviral activity of cytokines...
  11. ncbi Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection
    V Kumarasamy
    Makmal Kesihatan Awam Kebangsaan National Public Health Laboratory, Kementerian Kesihatan, Lot 1853 Kg Melayu, 47000 Sungai Buloh, Selangor, Malaysia
    J Virol Methods 140:75-9. 2007
    ..The results indicate that the commercial dengue NS1 antigen-capture ELISA may be superior to virus isolation and RT-PCR for the laboratory diagnosis of acute dengue infection based on a single serum sample...
  12. ncbi Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela
    Alvaro H Ramirez
    Instituto Venezolano de Investigaciones Cientificas, Centro de Microbiologia y Biologia Celular, Laboratorio de Biología de Virus, Caracas 1020A, Venezuela
    Diagn Microbiol Infect Dis 65:247-53. 2009
    ....
  13. pmc Human herpesvirus 8 LANA interacts with proteins of the mSin3 corepressor complex and negatively regulates Epstein-Barr virus gene expression in dually infected PEL cells
    A Krithivas
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21231, USA
    J Virol 74:9637-45. 2000
    ....
  14. pmc Cross-competition of CD8+ T cells shapes the immunodominance hierarchy during boost vaccination
    Wolfgang Kastenmuller
    Institute of Molecular Virology, Antigen specific Immunotherapy Clinical Cooperation Group, National Research Center for Environment and Health, 81675 Munich, Germany
    J Exp Med 204:2187-98. 2007
    ..Our findings provide a basis for novel strategies for how boost vaccination to defined antigens can be selectively improved. They give important new insights into the design of more efficient poxviral vectors for immunotherapy...
  15. ncbi Bioinformatics models for predicting antigenic variants of influenza A/H3N2 virus
    Yu Chieh Liao
    Division of Biostatistics and Bioinformatics, National Health Research Institutes, Zhunan 350, Taiwan
    Bioinformatics 24:505-12. 2008
    ..Continual and accumulated mutations in hemagglutinin (HA) protein of influenza A virus generate novel antigenic strains that cause annual epidemics...
  16. ncbi Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Diagn Microbiol Infect Dis 60:43-9. 2008
    ....
  17. pmc Structure and function of latency-associated nuclear antigen
    S C Verma
    Department of Microbiology and Tumor Virology Program of the Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Curr Top Microbiol Immunol 312:101-36. 2007
    ..Disruptions of LANA expression led to reduction in the episomal copies of the viral DNA, supporting its role in persistence of the viral DNA. The functions known so far suggest that LANA is a key player in KSHV-mediated pathogenesis...
  18. pmc Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen 1 mediates episome persistence through cis-acting terminal repeat (TR) sequence and specifically binds TR DNA
    M E Ballestas
    Department of Medicine, Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 75:3250-8. 2001
    ..Since LANA1 colocalizes with KSHV episomes along metaphase chromosomes, these results are consistent with a model in which LANA1 may bridge TR DNA to chromosomes during mitosis to efficiently segregate KSHV episomes to progeny nuclei...
  19. ncbi Human herpesvirus-8-encoded LNA-1 accumulates in heterochromatin- associated nuclear bodies
    L Szekely
    Microbiology and Tumor Biology Center, Karolinska Institute, S171 77, Stockholm, Sweden
    J Gen Virol 80:2889-900. 1999
    ..DNase but not RNase or detergent treatment of isolated nuclei destroys LNA-1 bodies. In advanced apoptotic cells LNA- 1 bodies remain intact but are not included in the apoptotic bodies themselves...
  20. pmc Rotavirus viremia and extraintestinal viral infection in the neonatal rat model
    Sue E Crawford
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    J Virol 80:4820-32. 2006
    ....
  21. pmc Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice
    David C Tscharke
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIH, Bethesda, MD 20892, USA
    J Virol 80:6318-23. 2006
    ..We then use these determinants to test if predicted conservation across orthopoxvirus species matches experimental observation and find an unexpectedly cross-reactive variant peptide encoded by ectromelia (mousepox) virus...
  22. pmc Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection
    Luc English
    Departement de pathologie et biologie cellulaire, Universite de Montreal, Succursale Centre Ville, Montreal, Quebec, Canada
    Nat Immunol 10:480-7. 2009
    ..Viral peptides in autophagosomes were further processed by the proteasome, which suggests a complex interaction between the vacuolar and MHC class I presentation pathways...
  23. ncbi Phylogenetic analysis of rabbit haemorrhagic disease virus in France between 1993 and 2000, and the characterisation of RHDV antigenic variants
    G Le Gall-Reculé
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches Avicoles et Porcines, Unité de virologie, Immunologie, Parasitologie, Aviaires et Cunicoles, Ploufragan, France
    Arch Virol 148:65-81. 2003
    ..Multiple sequence alignments of the VP60 gene and antigenic analysis with monoclonal antibodies demonstrated that these French isolates are two new isolates of the RHDV variant...
  24. pmc Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay
    A J Johnson
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
    J Clin Microbiol 38:1827-31. 2000
    ..4 to capture the specific inactivated viral antigens. Serum IgG was detected by using alkaline phosphatase-conjugated anti-human IgG (Fc portion)...
  25. pmc Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus up-regulates transcription of human telomerase reverse transcriptase promoter through interaction with transcription factor Sp1
    Subhash C Verma
    Department of Microbiology and Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Virol 78:10348-59. 2004
    ..Together, our results suggest that the interaction of LANA with Sp1 up-regulates the telomerase promoter, potentially contributing to the immortalization of KSHV-infected cells...
  26. pmc The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus supports latent DNA replication in dividing cells
    Jianhong Hu
    Division of Hematology Oncology and Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Virol 76:11677-87. 2002
    ..This notion is further strengthened by the unique modular structure of the KSHV TR element...
  27. ncbi The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus modulates cellular gene expression and protects lymphoid cells from p16 INK4A-induced cell cycle arrest
    Feng Qi An
    Division of Hematology Oncology and the Department of Molecular Biology and Microbiology, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 280:3862-74. 2005
    ..Under these conditions, LANA expression protects lymphoid cells from p16 INK4a-induced cell cycle arrest and induces S-phase entry...
  28. pmc The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL
    M R Wills
    Department of Medicine, University of Cambridge Clinical School, United Kingdom
    J Virol 70:7569-79. 1996
    ....
  29. pmc Induction of Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen by the lytic transactivator RTA: a novel mechanism for establishment of latency
    Ke Lan
    Department of Microbiology, University of Pennsylvania Medical School, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    J Virol 79:7453-65. 2005
    ..This describes a feedback mechanism by which LANA and RTA can regulate each other and is likely to be a key event in the establishment of KSHV latency...
  30. ncbi Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection
    J W Gratama
    Department of Clinical and Tumor Immunology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Blood 98:1358-64. 2001
    ..002). Thus, enumeration of HLA-restricted, CMV-specific CD8+ T cells in the grafts and monitoring of these T cells after SCT may constitute a rapid and sensitive tool to identify SCT recipients at risk for developing CMV disease...
  31. ncbi Attrition of T cell memory: selective loss of LCMV epitope-specific memory CD8 T cells following infections with heterologous viruses
    L K Selin
    Department of Pathology, University of Massachusetts Medical School, Worcester 01655, USA
    Immunity 11:733-42. 1999
    ..These results are consistent with a model for the immune system that accommodates memory T cell populations for multiple pathogens over the course of a lifetime...
  32. pmc Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen inhibits lytic replication by targeting Rta: a potential mechanism for virus-mediated control of latency
    Ke Lan
    Department of Microbiology and the Abramson Comprehensive Cancer Center, University of Pennsylvania Medical School, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    J Virol 78:6585-94. 2004
    ..This suggests that LANA may play a critical role in maintaining latency by controlling the switch between viral latency and lytic replication...
  33. ncbi Epstein-Barr virus nuclear antigen 3C targets p53 and modulates its transcriptional and apoptotic activities
    Fuming Yi
    Department of Microbiology and Tumor Virology Program, Abramson Comprehensive Cancer Center, University of Pennsylvania Medical School, 201E Johnson Pavilion, 3610 Hamilton Walk, PA 19104, USA
    Virology 388:236-47. 2009
    ..Thus, the interaction between the p53 and EBNA3C provides new insights into the mechanism(s) by which the EBNA3C oncoprotein can alter cellular gene expression in EBV associated human cancers...
  34. ncbi Serodiagnosis of human bocavirus infection
    Kalle Kantola
    Department of Virology, Haartman Institute, University of Helsinki, Finland
    Clin Infect Dis 46:540-6. 2008
    ..Moreover, nothing is known of HBoV immunity. We examined HBoV-specific systemic B cell responses and assessed their diagnostic use in young children with respiratory disease...
  35. ncbi Genetically destined potentials for N-linked glycosylation of influenza virus hemagglutinin
    Manabu Igarashi
    Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 001 0020, Japan
    Virology 376:323-9. 2008
    ..We hypothesize that influenza viruses maintained in natural reservoirs could have different potentials for sustained circulation, depending on their HA subtypes, if introduced into the human population...
  36. pmc SCFSkp2 complex targeted by Epstein-Barr virus essential nuclear antigen
    Jason S Knight
    Department of Microbiology and Abramson Comprehensive Cancer Center, University of Pennsylvania Medical School, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Mol Cell Biol 25:1749-63. 2005
    ....
  37. pmc An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Virol 81:9386-95. 2007
    ..In the CNS, EV71(S1-3') was isolated only from the spinal cord. These results indicate that EV71(S1-3') acts as an effective antigen, although this attenuated strain was still neurotropic when inoculated via the intravenous route...
  38. pmc Epstein-Barr virus nuclear antigen 3C interacts with and enhances the stability of the c-Myc oncoprotein
    Bharat G Bajaj
    Department of Microbiology and Tumor Virology Program, Abramson Comprehensive Cancer Center, University of Pennsylvania Medical School, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    J Virol 82:4082-90. 2008
    ..The inclusion of c-Myc in the group of cellular targets modulated by this domain further accentuates the importance of these critical residues of EBNA3C in bypassing the cell cycle checkpoints...
  39. ncbi A novel viral mechanism for dysregulation of beta-catenin in Kaposi's sarcoma-associated herpesvirus latency
    Masahiro Fujimuro
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    Nat Med 9:300-6. 2003
    ..Redistribution of GSK-3beta can therefore be a source of beta-catenin dysregulation in human cancers...
  40. ncbi Increased apoptosis and expression of tumor necrosis factor-alpha caused by infection of cultured human monocytes with dengue virus
    Luz M Espina
    Seccion de Virologia, Instituto de Investigaciones Clinicas Dr Americo Negrette, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela
    Am J Trop Med Hyg 68:48-53. 2003
    ....
  41. pmc Acetylation of the latency-associated nuclear antigen regulates repression of Kaposi's sarcoma-associated herpesvirus lytic transcription
    Fang Lu
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Virol 80:5273-82. 2006
    ..These findings support the role of LANA as a transcriptional repressor of lytic reactivation and provide evidence that lysine acetylation regulates LANA interactions with chromatin, Sp1, and ORF50 promoter DNA...
  42. pmc Target structures of the CD8(+)-T-cell response to human cytomegalovirus: the 72-kilodalton major immediate-early protein revisited
    F Kern
    Institut fur Medizinische Immunologie, Medizinische Fakultät der Humboldt Universität zu Berlin Charité, 10098 Berlin, Germany
    J Virol 73:8179-84. 1999
    ..In summary, our results suggest that IE-1 is far more important as a CD8(+)-T-cell target than current opinion suggests...
  43. ncbi Association between rotavirus infection and pancreatic islet autoimmunity in children at risk of developing type 1 diabetes
    M C Honeyman
    Autoimmunity and Transplantation Division, Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
    Diabetes 49:1319-24. 2000
    ..RV infection may therefore trigger or exacerbate islet autoimmunity in genetically susceptible children...
  44. ncbi Pathogenesis of experimental Ebola Zaire virus infection in BALB/c mice
    T R Gibb
    Diagnostic Systems, United States Army Medical Research Institute of Infectious Diseases (USAMRIID, Fort Detrick, Frederick, Maryland 21702-5011, USA
    J Comp Pathol 125:233-42. 2001
    ..The availability of immunodeficient, "gene-knockout" and transgenic mice will make the mouse model particularly useful for studying the early steps of Ebola pathogenesis...
  45. pmc Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion
    M Mathieu
    , CNRS-UPR 9053 1, , 91198 Gif-sur-Yvette Cedex, France
    EMBO J 20:1485-97. 2001
    ....
  46. ncbi IFN-alpha-conditioned dendritic cells are highly efficient in inducing cross-priming CD8(+) T cells against exogenous viral antigens
    Caterina Lapenta
    Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Rome, Italy
    Eur J Immunol 36:2046-60. 2006
    ..to IFN-alpha and GM-CSF (IFN-DC) are highly effective in inducing cross-priming of CD8(+ )T cells against viral antigens. We have investigated the mechanisms responsible for the special attitude of these DC and compared their ..
  47. ncbi Viral infection can induce the production of autoantibodies
    Ori Barzilai
    Center for Autoimmune Diseases, Department of Medicine B, Sheba Medical Center, Israel
    Curr Opin Rheumatol 19:636-43. 2007
    ..To review the current literature and summarize the main principles found between viral infections and the subsequent production of autoantibodies...
  48. ncbi Altered peptidase and viral-specific T cell response in LMP2 mutant mice
    L van Kaer
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139, USA
    Immunity 1:533-41. 1994
    ..These findings indicate that LMP2 influences antigen processing...
  49. ncbi HLA class II-restricted CD4+ T cell responses directed against influenza viral antigens postinfluenza vaccination
    Nancy A Danke
    Benaroya Research Institute at Virginia Mason, Seattle, WA 98101, USA
    J Immunol 171:3163-9. 2003
    ....
  50. ncbi Enumeration and characterization of virus-specific B cells by multicolor flow cytometry
    Virginia P Doucett
    Center for Comparative Medicine, University of California, Davis, County Rd 98, CA 95616, USA
    J Immunol Methods 303:40-52. 2005
    ..We conclude that viral HA can be used in conjunction with cell surface and intracytoplasmic stains in multicolor flow cytometry to provide detailed phenotypic and functional information on virus HA-specific B cells...
  51. ncbi Rotavirus antigenemia in patients with acute gastroenteritis
    Thea K Fischer
    Viral Gastroenteritis Team, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA or
    J Infect Dis 192:913-9. 2005
    ..In situations in which stool samples are not readily available (i.e., patients with severe dehydration or those recently recovered or deceased), serum testing by enzyme immunoassay offers a new and practical diagnostic tool...
  52. pmc Structural polymorphism of the major capsid protein of rotavirus
    J Lepault
    Laboratoire de Genetique des Virus, CNRS UPR 9053 1, Avenue de la Terrasse Bâtiment 14C, F 91198 Gif sur Yvette Cedex, France
    EMBO J 20:1498-507. 2001
    ..Our data show that the protonation state and thus the charge distribution are important switches governing the assembly of macromolecular assemblies...
  53. pmc Modulation of cellular and viral gene expression by the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus
    R Renne
    Division of Hematology Oncology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Virol 75:458-68. 2001
    ..These data demonstrate that LANA expression has effects on cellular and viral gene expression. We suggest that, whether direct or indirect in origin, these effects may play important roles in the pathobiology of KSHV infection...
  54. pmc Comparison of serologic assays and PCR for diagnosis of human herpesvirus 8 infection
    T J Spira
    Immunology Branch, Division of AIDS, STD, and TB Laboratory Research, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 38:2174-80. 2000
    ..As described above, only a titer of >/=1:40 would be considered HHV-8 positive...
  55. ncbi Molecular and antigenic characterization of rabbit hemorrhagic disease virus isolated in Cuba indicates a distinct antigenic subtype
    O Farnos
    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba
    Arch Virol 152:1215-21. 2007
    ..This is the second report on the molecular characterization of RHDVa circulating in the American region...
  56. pmc Determination of Kaposi's sarcoma-associated herpesvirus C-terminal latency-associated nuclear antigen residues mediating chromosome association and DNA binding
    Brenna Kelley-Clarke
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
    J Virol 81:4348-56. 2007
    ..In contrast to the essential role of N-terminal LANA chromosome targeting residues in DNA replication, deficient C-terminal chromosome association did not reduce LANA-mediated DNA replication...
  57. pmc Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi's sarcoma-associated herpesvirus LANA
    Meir Shamay
    Viral Oncology Program, Sidney Kimmel Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:14554-9. 2006
    ..The data provide an example of promoter-specific epigenetic DNA modification through viral protein recruitment of de novo Dnmt activity...
  58. pmc Defective cross-presentation of viral antigens in GILT-free mice
    Reshma Singh
    Department of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, 300 Cedar Street, Post Office Box 208011, New Haven, CT 06250 8011, USA
    Science 328:1394-8. 2010
    ..Efficient cross-presentation of disulfide-rich antigens requires a complex pathway involving GILT-mediated reduction, unfolding, and partial proteolysis, followed by translocation into the cytosol for proteasomal processing...
  59. ncbi Kaposi's sarcoma herpesvirus C-terminal LANA concentrates at pericentromeric and peri-telomeric regions of a subset of mitotic chromosomes
    Brenna Kelley-Clarke
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Ave, Boston, MA 02115, USA
    Virology 357:149-57. 2007
    ..These results are consistent with C-terminal LANA interacting with a cell factor that concentrates at pericentromeric and peri-telomeric regions of mitotic chromosomes...
  60. pmc Continuous recruitment of naive T cells contributes to heterogeneity of antiviral CD8 T cells during persistent infection
    Vaiva Vezys
    Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Exp Med 203:2263-9. 2006
    ..These findings reveal a novel role for antigen in maintaining virus-specific CD8 T cells during persistent infection and provide insight toward understanding T cell differentiation in chronic infection...
  61. pmc In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes
    Giovanna Zanoni
    Section of Immunology, Department of Pathology, University of Verona, Verona, Italy
    PLoS Med 3:e358. 2006
    ..Involvement of infectious agents and innate immunity has been suggested but never proven. Molecular mimicry is one of the mechanisms that links infection and autoimmunity...
  62. pmc Combinations of polyclonal or monoclonal antibodies to proteins of the outer membranes of the two infectious forms of vaccinia virus protect mice against a lethal respiratory challenge
    Shlomo Lustig
    Laboratory of Viral Diseases, National Institutes of Health, 4 Memorial Dr, MSC 0445, Bethesda, MD 20892 0445, USA
    J Virol 79:13454-62. 2005
    ..These studies suggest that antibodies to two or three viral membrane proteins optimally derived from the outer membranes of IMV and EV, may be beneficial for prophylaxis or therapy of orthopoxvirus infections...
  63. pmc Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections
    Pei Yun Shu
    Division of Research Development and Laboratory Diagnosis, Center for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
    Clin Diagn Lab Immunol 10:622-30. 2003
    ..These findings suggested that NS1 serotype-specific IgG ELISA could be reliably applied for serodiagnosis and seroepidemiological study of dengue virus infection...
  64. pmc Vaccinia virus H3L envelope protein is a major target of neutralizing antibodies in humans and elicits protection against lethal challenge in mice
    D Huw Davies
    Center for Virus Research, Department of Molecular Biology and Biochemistry, McGaugh Hall, University of California, Irvine 92697, USA
    J Virol 79:11724-33. 2005
    ..02). Together, these data show that H3L is a major target of the human anti-poxvirus antibody response and is likely to be a key contributor to protection against poxvirus infection and disease...
  65. ncbi Confronting complexity: real-world immunodominance in antiviral CD8+ T cell responses
    Jonathan W Yewdell
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
    Immunity 25:533-43. 2006
    ..Here, I review work that has extended immunodominance studies to viruses of greater complexity and to the real world of human antiviral immunity...
  66. ncbi Expression cloning for the discovery of viral antigens and epitopes recognized by T cells
    David M Koelle
    Department of Medicine, University of Washington, Seattle, WA 98104, USA
    Methods 29:213-26. 2003
    ..b>Viral antigens are frequently used in cross-sectional and longitudinal studies of the immune response in pathogenesis ..
  67. ncbi Physical and functional interactions between STAT3 and Kaposi's sarcoma-associated herpesvirus-encoded LANA
    Ryuta Muromoto
    Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    FEBS Lett 580:93-8. 2006
    ..These data demonstrate that LANA is a transcriptional co-activator of STAT3, and may have implications for the pathogenesis of KSHV-associated diseases...
  68. pmc Typing of human rotaviruses: nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure
    Mustafizur Rahman
    ICDDR, B Centre for Health and Population Research, Mohakhali, Dhaka 1212, Bangladesh
    Virol J 2:24. 2005
    ..9%) of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed...
  69. ncbi Activation and inactivation of antiviral CD8 T cell responses during murine pneumovirus infection
    Erwin A W Claassen
    Department of Immunology, Faculty of Veterinary Science, University of Utrecht, Utrecht, The Netherlands
    J Immunol 175:6597-604. 2005
    ..This deficient phenotype was retained up to 96 days postinfection, similar to the situation in the lungs of human RSV-infected mice. The data suggest that PVM suppresses T cell effector functions in the lungs...
  70. pmc Epitope determinants of a chimpanzee dengue virus type 4 (DENV-4)-neutralizing antibody and protection against DENV-4 challenge in mice and rhesus monkeys by passively transferred humanized antibody
    CHING JUH LAI
    Laboratory of Infectious Diseases, National Institutes of Health, 50 South Drive MSC 8005, Bethesda, MD 20892, USA
    J Virol 81:12766-74. 2007
    ..This substitution was also present in all naturally occurring isolates belonging to DENV-4 genotype III. These studies have important implications for possible antibody-mediated prevention of DENV infection...
  71. pmc Immunization with live attenuated influenza viruses that express altered NS1 proteins results in potent and protective memory CD8+ T-cell responses
    Scott N Mueller
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Virol 84:1847-55. 2010
    ..These results show that live attenuated influenza viruses expressing truncated NS1 proteins can prime protective immunity and may have implications for the design of novel modified live influenza virus vaccines...
  72. ncbi Human B cells secrete granzyme B when recognizing viral antigens in the context of the acute phase cytokine IL-21
    Magdalena Hagn
    Laboratory of Tumor and B Cell Immunology, Institute of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, 89081 Ulm, Germany
    J Immunol 183:1838-45. 2009
    ..Our data may also explain the elevated serum GrB levels found in the early phase of various viral diseases...
  73. pmc Epstein-Barr virus nuclear antigen 3C regulates cyclin A/p27 complexes and enhances cyclin A-dependent kinase activity
    Jason S Knight
    Department of Microbiology and Abramson Comprehensive Cancer Center, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104, USA
    J Virol 78:1981-91. 2004
    ....
  74. pmc Immunization with different viral antigens alters the pattern of T cell exhaustion and latency in herpes simplex virus type 1-infected mice
    Sariah J Allen
    Center for Neurobiology and Vaccine Development, Ophthalmology Research Laboratories, Department of Surgery, CSMC Burns and Allen Research Institute, Los Angeles, California 90048, USA
    J Virol 84:12315-24. 2010
    ..In this study, we have shown for the first time that both PD-1 and Tim-3 contribute to T cell exhaustion and an increase of latency in the TG of latently infected mice...
  75. ncbi Antigenic structure of flavivirus proteins
    John T Roehrig
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
    Adv Virus Res 59:141-75. 2003
    ..To prepare effective and inexpensive subunit vaccines, we will need to continue to better understand these structure-function relationships of flavivirus proteins...
  76. ncbi Cutting edge: CCR7+ and CCR7- memory T cells do not differ in immediate effector cell function
    Heike Unsoeld
    Department of Immunology, Institute for Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany
    J Immunol 169:638-41. 2002
    ..These results question the proposed concept of differential effector cell function of CCR7(+) and CCR7(-) memory T cells...
  77. pmc Inflation and long-term maintenance of CD8 T cells responding to a latent herpesvirus depend upon establishment of latency and presence of viral antigens
    Anna Lang
    Department of Immunobiology and Arizona Center on Aging, University of Arizona College of Medicine, Tucson, AZ 85724, USA
    J Immunol 183:8077-87. 2009
    ..Moreover, results with antiviral drugs suggest that this approach could be considered as a treatment modality for maintaining T cell diversity and/or function in old age...
  78. ncbi The KSHV latency-associated nuclear antigen: a multifunctional protein
    Takashi Komatsu
    Department of Medicine, Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Ave, Boston, MA 021115, USA
    Front Biosci 7:d726-30. 2002
    ..Here we review LANA1's roles in KSHV infection. Topics discussed include LANA1's roles in episome persistence, regulation of transcription and interaction with cellular proteins...
  79. pmc KSHV reactivation from latency requires Pim-1 and Pim-3 kinases to inactivate the latency-associated nuclear antigen LANA
    Fang Cheng
    Genome Scale Biology Program, Institute of Biomedicine, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland
    PLoS Pathog 5:e1000324. 2009
    ....
  80. ncbi Targeting HIV-1 Gag into the defective ribosomal product pathway enhances MHC class I antigen presentation and CD8+ T cell activation
    Andreas Goldwich
    Institute of Clinical and Molecular Virology, Department of Dermatology, University Hospital of Erlangen, Germany
    J Immunol 180:372-82. 2008
    ..These results further underline the role of the defective ribosomal product pathway in adaptive immunity...
  81. ncbi Identification of immunodominant VP1 linear epitope of enterovirus 71 (EV71) using synthetic peptides for detecting human anti-EV71 IgG antibodies in Western blots
    D G W Foo
    Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Clin Microbiol Infect 14:286-8. 2008
    ..The amino-acid sequence of SP32 was highly specific for detecting EV71 strains in western blot analysis, and showed no immunoreactivity with monoclonal antibodies raised against other enteroviruses, e.g., CA9 and Echo 6...
  82. pmc Functional dissection of latency-associated nuclear antigen 1 of Kaposi's sarcoma-associated herpesvirus involved in latent DNA replication and transcription of terminal repeats of the viral genome
    Chunghun Lim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Virol 76:10320-31. 2002
    ..LANA1 interacted with components of the origin recognition complexes (ORCs), similar to Epstein-Barr virus nuclear antigen 1. Our data suggest that LANA1 recruits ORCs to KSHV TRs for latent replication of the viral genome...
  83. pmc Spontaneous molecular reactivation of herpes simplex virus type 1 latency in mice
    Lawrence T Feldman
    Department of Microbiology and Immunology, University of California Medical Center, Los Angeles, CA 90024, USA
    Proc Natl Acad Sci U S A 99:978-83. 2002
    ..In the absence of detectable infectious virus in these ganglia, we propose the term "spontaneous molecular reactivation" to describe this ongoing process...
  84. ncbi Cowpox virus evades CTL recognition and inhibits the intracellular transport of MHC class I molecules
    Anindya Dasgupta
    Oregon Health and Science University, Vaccine and Gene Therapy Institute, Beaverton, OR 97006, USA
    J Immunol 178:1654-61. 2007
    ..The absence of this immune evasion mechanism in VV likely limits virulence without compromising immunogenicity...
  85. ncbi Pathogenesis of rabies
    B Dietzschold
    Department of Microbiology, Center for Neurovirology, Thomas Jefferson University, Philadelphia, PA, USA
    Curr Top Microbiol Immunol 292:45-56. 2005
    ....
  86. pmc Close but distinct regions of human herpesvirus 8 latency-associated nuclear antigen 1 are responsible for nuclear targeting and binding to human mitotic chromosomes
    T Piolot
    Service de Microbiologie-EA 2391, , 75571 Paris Cedex 12, France
    J Virol 75:3948-59. 2001
    ..The presence of putative phosphorylation sites in the chromosome binding sites of LNA1 and EBNA1 suggests that their activity may be regulated by specific cellular kinases...
  87. ncbi Characterisation of a novel lyssavirus isolated from Pteropid bats in Australia
    A R Gould
    CSIRO, Australian Animal Health Laboratory, Geelong, Victoria
    Virus Res 54:165-87. 1998
    ..Phylogenetic studies of N protein sequences showed clearly that ABLV is an unrecognised member of the Lyssavirus genus and represents a new genotype, genotype 7...
  88. pmc Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype
    M I Gómara
    Clinical Microbiology and Public Health Laboratory, Addenbrooke s Hospital, Cambridge CB2 2QW, United Kingdom
    J Clin Microbiol 39:3796-8. 2001
    ....
  89. pmc Rapid detection of antigenic diversity of Akabane virus isolates by dot immunobinding assay using neutralizing monoclonal antibodies
    K Yoshida
    Laboratory of Clinical Virology, Kyushu Research Station, National Institute of Animal Health, Chuzan, Kagoshima, Japan
    Clin Diagn Lab Immunol 5:192-8. 1998
    ..The results show that DIA can be used to effectively compare the antigenicities of AKA virus isolates within a few hours, even with lesser amounts of virus culture than is required for other assays...
  90. pmc Accumulation of heterochromatin components on the terminal repeat sequence of Kaposi's sarcoma-associated herpesvirus mediated by the latency-associated nuclear antigen
    Shuhei Sakakibara
    Department of Microbiology C1, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    J Virol 78:7299-310. 2004
    ..Taken together, these observations indicate that LANA recruits heterochromatin components to the viral genome, which may lead to the establishment of viral latency and govern the transcription program...
  91. ncbi Development and application of monoclonal antibodies against avian influenza virus nucleoprotein
    Ming Yang
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada
    J Virol Methods 147:265-74. 2008
    ..The two mAbs with different specificities are appropriate for developing various tests for diagnosis of AI infection...
  92. ncbi Direct visualization of cytomegalovirus-specific T-cell reconstitution after allogeneic stem cell transplantation
    K Cwynarski
    CRC Institute for Cancer Studies, University of Birmingham, Edgbaston, United Kingdom
    Blood 97:1232-40. 2001
    ..It should allow prediction of CMV reactivation in individual patients and assist in the development of adoptive T-cell immunotherapy...
  93. pmc Epstein-Barr virus latent antigen 3C can mediate the degradation of the retinoblastoma protein through an SCF cellular ubiquitin ligase
    Jason S Knight
    Department of Microbiology and the Tumor Virology Program, Abramson Comprehensive Cancer Center, University of Pennsylvania, 201E Johnson Pavilion, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:18562-6. 2005
    ..That EBNA3C has exploited this association suggests that other human malignancies might use a similar strategy to regulate the Rb protein...
  94. ncbi Addition of a prominent epitope affects influenza A virus-specific CD8+ T cell immunodominance hierarchies when antigen is limiting
    Misty Rayna Jenkins
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    J Immunol 177:2917-25. 2006
    ....
  95. ncbi Receptor-binding domain of severe acute respiratory syndrome coronavirus spike protein contains multiple conformation-dependent epitopes that induce highly potent neutralizing antibodies
    Yuxian He
    Viral Immunology Laboratory, Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10021, USA
    J Immunol 174:4908-15. 2005
    ..005 to 6.569 microg/ml. Therefore, the RBD of SARS S protein contains multiple conformational epitopes capable of inducing potent neutralizing Ab responses, and is an important target site for developing vaccines and immunotherapeutics...
  96. ncbi Cytotoxic T lymphocytes, chemokines and antiviral immunity
    D A Price
    Nuffield Dept of Clinical Medicine and Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK OX3 9DU
    Immunol Today 20:212-6. 1999
    Evidence that CD8+ CTLs produce chemokines following engagement of viral antigens, and that MIP-1alpha is required for an inflammatory response to virus challenge, suggests that these molecules are key elements in the generation of ..
  97. pmc The ATM/ATR signaling effector Chk2 is targeted by Epstein-Barr virus nuclear antigen 3C to release the G2/M cell cycle block
    Tathagata Choudhuri
    Department of Microbiology and the Tumor Virology Program, Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    J Virol 81:6718-30. 2007
    ..Overall, our data suggest that EBNA3C can directly regulate the G2/M component of the host cell cycle machinery, allowing for the release of the checkpoint block...
  98. pmc Long-lived memory T lymphocyte responses after hantavirus infection
    Heather L Van Epps
    Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Exp Med 196:579-88. 2002
    ..The frequencies of PUUV-specific CTL were comparable to or exceeded those found in other viral systems including influenza, EBV and HIV, in which CTL responses may be boosted by periodic reinfection or virus persistence...
  99. pmc Multicenter comparison of the digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of cytomegalovirus viremia
    T Mazzulli
    Mt Sinai Hospital, Toronto, Ontario, Canada
    J Clin Microbiol 37:958-63. 1999
    ..Further evaluation to determine its role in predicting CMV disease and for monitoring the therapeutic response to anti-CMV therapy is needed...
  100. ncbi A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant
    L Capucci
    Department of Biotechnology, Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia, Brescia, Italy
    Virus Res 58:115-26. 1998
    ..Bs89 drops to 70%, suggesting that this region probably contains the epitope recognized by mAb 1H8. In addition, this paper presents preliminary data concerning the amino acids of VP60 involved in the hemagglutination site of the virus...
  101. pmc Current advances in dengue diagnosis
    Pei Yun Shu
    Division of Laboratory Research and Development, Center for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
    Clin Diagn Lab Immunol 11:642-50. 2004

Research Grants66

  1. CFI Pathogen Inactivation Technology
    Trevor P Castor; Fiscal Year: 2010
    ..Some enzyme immunoassays used for routine screening may detect viral antigens or antibodies, but not the infectious agents themselves...
  2. Novel vaccine to CMV based on a disc virus
    Alistair McGregor; Fiscal Year: 2010
    ..regardless of virus dosage used, but highly immunogenic inducing antibody and T-cell responses to an array of viral antigens. Additionally, we propose that the effectiveness of such a DISC vaccine against CMV will be further augmented ..
  3. Antigen presentation by epithelial stem cells to promote life long immunity
    MARIE CLAIRE ELISABETH GAUDUIN; Fiscal Year: 2013
    ..there are two necessary features for a successful vaccine: 1) life-long stimulation of the immune system with viral antigens;and 2) a targeted immune response at the site of primary replication of HIV...
  4. Point of care microfluidic diagnosis system for rapid and sensitive detection of
    Michel G Bergeron; Fiscal Year: 2013
    ..that more than a dozen different viruses infect human upper airways, yet current diagnostic methods based on viral antigens often lack the required sensitivity, while culture-based methods are extremely slow...
  5. Lambda phage as a scaffold for influenza immunization
    Stephen Dewhurst; Fiscal Year: 2009
    ..is that influenza A virus vaccine approaches can be improved, by using lambda phage as a scaffold to display viral antigens in a highly immunogenic context...
  6. Influenza Immunity: Protective Mechanisms Against Pandemic Respiratory Virus
    MARK MORRIS DAVIS; Fiscal Year: 2013
    ..While the human immune system has the intrinsic capability of coping with highly diversified viral antigens with its enormous antibody repertoire (estimated at a magnitude of 1011), it is our hypothesis that this ..
  7. Gene Expression in HSV-1 Latency After Corneal Infection
    Paul R Kinchington; Fiscal Year: 2013
    ..Our first specific aim tests the hypothesis that CD8+T cell recognition of viral antigens made before viral DNA replication is critical to effectively block reactivation in a non-cytolytic manner...
  8. Regulation of antibody diversification and production in HIV-1 infection
    Andrea Cerutti; Fiscal Year: 2012
    ..the antigen-binding variable region of antibodies, thereby providing the structural correlate for selection by viral antigens of higher affinity IgG and IgA mutants...
  9. Role of B-cells and CNS antibodies in modulating chronic microglial activation
    James R Lokensgard; Fiscal Year: 2013
    ..by brain-infiltrating cells of the peripheral immune system, as well as by their mediators, rather than simply viral antigens themselves...
  10. Autophagy in Antiviral Immunity
    Akiko Iwasaki; Fiscal Year: 2013
    ..In addition, recent reports indicate that autophagy delivers endogenous viral antigens to the MHC class II loading compartment, allowing activation of CD4 T cells...
  11. Immune factors in vaccine-enhanced pandemic H1N1 influenza disease
    Amy L Vincent; Fiscal Year: 2013
    ..The results will also focus future mechanistic studies into the critical viral antigens (e.g. specific proteins and epitopes targeted) and host immune factors (e.g...
  12. Targeted Dendritic Cell Activation through Multi-Adjuvant Liposomes
    Gretchen Jimenez; Fiscal Year: 2013
    ..The formulations, including testing of single adjuvants, will be assessed with influenza and Herpes Simplex 2 viral antigens in vivo in proven mouse models...
  13. Generation and Function of NK Cell Memory
    Ulrich H von Andrian; Fiscal Year: 2013
    ..to gain a deeper understanding of the induction and regulation of immunological events that are elicited by viral antigens in lymph nodes (LNs)...
  14. Anti-viral immune responses in lymph node
    Ulrich H von Andrian; Fiscal Year: 2012
    ..to gain a deeper understanding of the induction and regulation of immunological events that are elicited by viral antigens in lymph nodes (LNs)...
  15. Mechanisms of CD8+ T cell Immune surveillance in human genital skin and mucosa af
    Jia Zhu; Fiscal Year: 2012
    ..endings where HSV virion particles are released, and that HSV-2 specific CD8+ T cells frequently encounter viral antigens. Recent studies have shown that over 80% of HSV-2 reactivations are subclinical and of short duration, ..
  16. BCG as an HIV vaccine vector
    Anna Lise Williamson; Fiscal Year: 2011
    ..Despite these advantages there are also problems expressing viral antigens in BCG, which result in low immunogenicity and genetic instability...
  17. Targeting Viral Envelope Glycoproteins with Synthetic Antibodies
    Jonathan Lai; Fiscal Year: 2013
    ..In Aim 1, we propose to develop synthetic antibody libraries focused toward viral antigens based on the promiscuous germline segment VH1-69...
  18. Innate Immunity and Herpes Simplex Infection
    ROBERT WILLIAM FINBERG; Fiscal Year: 2010
    ..The overall hypothesis of the Project is that pattern recognition proteins respond to particular viral antigens and that those responses are regulated by other viral proteins and it is the effect of the host cytokines ..
  19. In vivo suppression of SIV-mediated immune activation
    Anna Aldovini; Fiscal Year: 2013
    ..can be induced by a variety of mechanisms, including persistent stimulation of immune responses by viral antigens, and/or production of immunoactivating cytokines and chemokines...
  20. CMV Therapeutic Vaccine for the Treatment of Glioblastoma
    John H Sampson; Fiscal Year: 2012
    ..reactivation of human cytomegalovirus (CMV) provides an opportunity to leverage the well characterized viral antigens of CMV as tumor-specific targets...
  21. Protective roles of CTL responses to HIV-1 associated self epitope
    BETH DEIRDRE JAMIESON KARAVODIN; Fiscal Year: 2010
    ..role in the overall long-term immunologic control of HIV-1 viremia by augmenting existing CTL responses to viral antigens. To test this hypothesis, we intend to: Aim 1) Characterize HLA-B*5701 restricted self-epitopes up-regulated ..
  22. Role of B Cells in Murine Gammaherpesvirus-68 Latency
    Samuel H Speck; Fiscal Year: 2013
    ..Aim 2. Characterization of MHV68 latency programs and viral antigens: 2.a. Identify viral genes expressed in distinct latently infected B cell and macrophage populations; 2.b...
  23. Point-of-Care HIV Antigen/Antibody Diagnostic Device
    Michael J Lochhead; Fiscal Year: 2011
    ..There are currently no FDA-approved point-of-care (POC) tests that directly target HIV viral antigens. An HIV-1 antigen/antibody (Ag/Ab) combination assay - the so-called "4th generation" immunoassay - in an ..
  24. Inhibitors of HIV-induced downregulation of HLA class I
    Juan Lama; Fiscal Year: 2013
    ..The viral protein Nef induces MHC-I downmodulation and interferes with the presentation of viral antigens, making HIV-infected cells less sensitive to killing by CD8-positive CTLs...
  25. Papillomavirus Host Interation
    Neil D Christensen; Fiscal Year: 2013
    ..For example, we do not yet know: (i) which viral antigens can induce the most effective immune-mediated clearance of HPV infections;(ii) which viral epitopes produce ..
  26. A high-sensitivity low-cost multiplexed immunoassay platform based on 2-dimension
    Paul Yager; Fiscal Year: 2013
    ..The aim of this project is to apply the instrument-free 2DPN platform to detection of viral antigens;these devices will greatly increase sensitivity, while maintaining the speed, ease of use, and low cost of ..
  27. Training a New Generation of Vaccinologists
    MARK JOSEPH MULLIGAN; Fiscal Year: 2013
    ..1) Pathogenesis &Vaccine Discovery;2) Innate &Adaptive Immune Responses to Bacterial, Protozoal, &Viral Antigens;3) Vaccine Epidemiology &Health Policy;and 4) Translation, Delivery &Clinical Trials...
  28. Epithelial Cells as Mucosal Adjuvant for Life Long Immunity
    Marie Claire Gauduin; Fiscal Year: 2009
    ..there are two necessary features for a successful vaccine: 1) life-long stimulation of the immune system with viral antigens; and 2) a targeted immune response at the site of primary replication of HIV...
  29. HSV encoded viral functions
    Bin He; Fiscal Year: 2012
    ..It is well documented that upon infection immature dendritic cells take up viral antigens, undergo maturation...
  30. Development of an effective DISC vaccine strategy against congenital CMV
    Alistair McGregor; Fiscal Year: 2013
    ..infectious single cycle (DISC) vaccine strategy results in a virs that can infect cells to express an array of viral antigens and induce an immune response but does not produce infectious virus...
  31. Viral Evolution and Humoral Immune Response to Dual HIV-1 Infection
    Phillipe N Nyambi; Fiscal Year: 2013
    ..the occurrence of dual infection provides a unique opportunity to investigate immune responses to multiple viral antigens and to study whether the host immune response is broadened when challenged with multiple, diverse antigens ..
  32. Viral Evolution and Humoral Immune Response to Dual HIV-1 Infection
    Phillipe Nyambi; Fiscal Year: 2009
    ..the occurrence of dual infection provides a unique opportunity to investigate immune responses to multiple viral antigens and to study whether the host immune response is broadened when challenged with multiple, diverse antigens ..
  33. Point-of-Care HIV Antigen/Antibody Diagnostic Device
    Michael J Lochhead; Fiscal Year: 2013
    ..There are currently no FDA-approved point-of-care (POC) tests that directly target HIV viral antigens. An HIV-1 antigen/antibody (Ag/Ab) combination assay - the so-called "4th generation" immunoassay - in an ..
  34. CD* T cell responses to gamma-herpesviral infection
    Michael L Freeman; Fiscal Year: 2010
    ..Latently-infected cells and cells undergoing viral reactivation from latency could present disparate viral antigens to circulating CDS T cells...
  35. DENDRITIC CELLS IN ATOPIC AND VIRUS-INDUCED AIRWAY DISEASE
    Mitchell H Grayson; Fiscal Year: 2012
    ..FceRI expression on DC will be studied, as will the role of DC FceRI expression to generate IgE against non-viral antigens. PUBLIC HEALTH RELEVANCE...
  36. CD4+ T cell affinity for self and foreign antigens in the CNS
    Brian D Evavold; Fiscal Year: 2013
    ..a major advancement in assessment in the frequency and affinity of CD4+ T cells directed against myelin or viral antigens. Therefore for the first time, it is possible to track the range of affinities of a CD4+ T cells response ..
  37. Effects of Probiotic Lactobacilli on Rotavirus Immunity
    Lijuan Yuan; Fiscal Year: 2006
    ..by triggering toll-like receptors (TLR) and innate immune responses? and 2) Do they act synergistically with viral antigens in the stimulation of TLR expression/activation to induce protective innate and adaptive immunity against ..
  38. Targeting pDCs for the Generation of Effective Anti-HCV CD8+ T-Cell Immunity
    John Connolly; Fiscal Year: 2009
    ..that pDCs have specialized antigen processing compartments (MICs) which permit them to rapidly cross-present viral antigens and stimulate protective CD8+ T-cell responses...
  39. Accumulation of VH replacement products in HIV patients
    Zhixin Zhang; Fiscal Year: 2012
    ..cells expressing VH replacement products during anti-HIV response are two different processes triggered by HIV viral antigens during chronic HIV infection...
  40. Analysis of Sin Nombre virus inhibition in lung cells
    Eric Barklis; Fiscal Year: 2005
    ..Studies have demonstrated marked accumulations of viruses and extremely high levels of viral antigens in the lungs of HPS patients, consistent with their involvement in the deterioration of lung function...
  41. T cell regulation: CD4+CD25+ FOXP3+ T cells in acute and chronic HIV-1 infection
    Marylyn Martina Addo; Fiscal Year: 2010
    ..T cell responses directed against tumors and allografts as well as against parasitic, bacterial, fungal, and viral antigens. Tregs are thought to play a prominent role in maintaining the delicate balance between an immune response ..
  42. HEPATITIS B VIRUS GENE EXPRESSION
    Alan McLachlan; Fiscal Year: 1991
    ..liver damage resulting from HBV Infection is mediated by a cellular immune response to hepatocytes expressing viral antigens, an amphotropoic retroviral expression system will be developed to generate autologous human cytolytic T-..
  43. THE SECRETORY IGA SYSTEM OF THE EYE
    David Sullivan; Fiscal Year: 1993
    ..virus on subsequent ocular response to viral challenge; and f) functional role of IgA antibodies to viral antigens in ocular defense...
  44. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2000
    ..related lentiviruses involve 1) induction of mucosal immunity and 2) immunological responses against multiple viral antigens. To this end, we have developed replication-competent poliovirus recombinants that can carry and express ..
  45. Evaluation of the aerosol measles vaccine in children
    ROSA WONG CHEW; Fiscal Year: 2007
    ..5) To compare the cellular responses of infants to bacterial and viral antigens according to the route of measles vaccine administration. Measles is still a problem of public health...
  46. DEVELOPMENT OF A SYNTHETIC VACCINE TO RETROVIRUSES
    John Elder; Fiscal Year: 1990
    ..affinity of monoclonal antibodies generated against synthetic peptide antigens to those made against the viral antigens. We will thus be able to predict peptide structures which best mimic the native antigen...
  47. DETECTION OF VIRAL TRANSACTIVATORS IN PRIMARY CELLS
    Roger Strair; Fiscal Year: 1993
    ..pathological effect; 3) the presence of viral nucleic acid sequences in the diseased cells; 4) the presence of viral antigens in malignant cells; 5) the prevalence of the disease in patients with serologic evidence of infection with the ..
  48. STRUCTURAL BASIS OF FUNCTIONAL CLASS II GENE EXPRESSION
    Laurie Glimcher; Fiscal Year: 1992
    ..The ability of these transgenic mice to respond to foreign protein antigens, tumor antigens, viral antigens and allogeneic and xenogeneic antigens can then be evaluated...
  49. STRUCTURAL BASIS OF FUNCTIONAL CLASS II GENE EXPRESSION
    Laurie Glimcher; Fiscal Year: 2001
    ..The ability of these transgenic mice to respond to foreign protein antigens, tumor antigens, viral antigens and allogeneic and xenogeneic antigens can then be evaluated...
  50. ONTOGENY OF MEASLES IMMUNITY IN INFANTS
    Ann Arvin; Fiscal Year: 2006
    ..vaccine during the first year of life, in order to enhance basic knowledge about the infant immune response to viral antigens, and to generate information that will have practical relevance for the global eradication of measles...
  51. ONTOGENY OF MEASLES IMMUNITY IN INFANTS
    Hayley Gans; Fiscal Year: 2009
    ..vaccine during the first year of life, in order to enhance basic knowledge about the infant immune response to viral antigens, and to generate information that will have practical relevance for the global eradication of measles...
  52. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..However, many viral antigens and human blood proteins are anchored to membranes and require glycosylation for their stability and ..
  53. MACROPHAGE ANTIGEN PROCESSING OF HIV SUBTYPES
    Kenneth Knox; Fiscal Year: 2005
    ..to T-tropic or M-tropic HIV are equally able to induce a primary CTL response, indicating that processing of viral antigens is occurring after exposure to both strains...
  54. HUMAN T LYMPHOCYTE RESPONSE TO VIRAL ANTIGENS
    Thomas Braciale; Fiscal Year: 1991
    ..In addition, these studies should help to elucidate the similarities and differences in the mechanism of antigen presentation to "helper" and "killer" T lymphocytes and thereby provide an important framework for future vaccine design...
  55. FACS ANALYSIS OF ANTIVIRAL DRUGS ON HCMV REPLICATION
    JAMES MCSHARRY; Fiscal Year: 2000
    ..cytomegalovirus; and 2) to demonstrate the feasibility of using fluoroochrome labeled monoclonal antibodies to viral antigens and flow cytometry to begin to determine the mode of action of these antiviral compounds...
  56. VSV INFECTION OF THE CENTRAL NERVOUS SYSTEM
    CAROL REISS; Fiscal Year: 2005
    ..major histocompatibility antigens, cannot be recognized by T lymphocytes performing immune surveillance for viral antigens. Viral replication can modify cellular metabolism, sometimes leading to initiation of apoptosis, resulting in ..
  57. Adaptations of Human Herpesvirus-7 to Salivary Glands
    Stephen Dewhurst; Fiscal Year: 2005
    ..is associated with persistence of viral genomes in salivary gland (SG) tissues, and chronic expression of viral antigens in these sites...
  58. ANALYSIS OF VIRAL AND H-2 ANTIGEN INTERACTIONS
    DOUGLAS LYLES; Fiscal Year: 1991
    ..These viral antigens and H-2 proteins can be purified and incorporated into synthetic phospholipid membranes (liposomes) which are ..
  59. Inducing and targeting of EBV lytic antigens in lymphoma
    Sven de Vos; Fiscal Year: 2006
    ..EBV lytic antigen expression in EBV-related malignancies, initiating the expression of viral TK and other viral antigens, thereby rendering the tumor cells susceptible to killing by the nucleoside analogue Gancyclovir and cells of ..
  60. Targeting early proteins for therapeutic vaccines
    Jay Rappaport; Fiscal Year: 2001
    ..Immune responses to early and late viral antigens, including the immunogen, will be monitored prior to HAART, during HAART and after discontinuation of HAART...
  61. IMMUNOPATHOLOGY OF VIRUS INDUCED DEMYELINATION
    Mauro Dal Canto; Fiscal Year: 2001
    ..to this demyelinating disease is its ability to develop a delayed-type hyper-sensitivity (DTH) response to viral antigens. Both immunological parameters, such as the ability to develop a DTH response to virus, as well as factors ..
  62. MULTIPLEXED VIRAL DETECTION IFA BY FLOW CYTOMETRY
    Daniel Collins; Fiscal Year: 2000
    ..Contamination by these pathogens is determined by the detection of viral antigens or anti-viral antibodies using EIA technology...
  63. Long Term Effects of Influenza Virus on T Cell Migration
    LINDA CAULEY; Fiscal Year: 2007
    ..Our preliminary data show that processed viral antigens are presented in v/vofor several weeks (and maybe months) after pulmonary influenza virus infection...
  64. Non-Integrating Lentiviral Vectors: Potential as Vaccines
    Mary Klotman; Fiscal Year: 2006
    ..Chronic presentation of low levels of viral antigens in the setting of a viral infection provides protection...
  65. ANALYSIS OF C-ADE AND FCR-ADE IN HIV & SIV INFECTION
    William Mitchell; Fiscal Year: 1991
    ..Virus Type 1, (HIV-1) induces a strong humoral immune response in humans and animals to virtually all of its viral antigens. Despite neutralizing antibody responses in humans and chimpanzees, humoral immune defenses fail to protect ..
  66. The Glossopharyngeal Nerve in Neuroimmune Connections
    Horacio Romeo; Fiscal Year: 2005
    ..are secondary lymphoid organs that play a crucial role in initiating immune responses against bacterial and viral antigens. It is well established that the central nervous system can modulate several aspects of immune function...