protozoan antigens

Summary

Summary: Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.

Top Publications

  1. pmc Estimating medium- and long-term trends in malaria transmission by using serological markers of malaria exposure
    C J Drakeley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 102:5108-13. 2005
  2. pmc Improved diagnostic testing and malaria treatment practices in Zambia
    Davidson H Hamer
    Center for International Health and Development, Boston University School of Public Health, Boston, Mass 02118, USA
    JAMA 297:2227-31. 2007
  3. pmc Rapid diagnostic tests for malaria parasites
    Anthony Moody
    Department of Clinical Parasitology, Hospital for Tropical Diseases, University Collge Hospital, London, United Kingdom
    Clin Microbiol Rev 15:66-78. 2002
  4. ncbi Immune effector mechanisms in malaria
    K Marsh
    KEMRI Centre for Geographic Medicine Research Coast CGMRC, PO Box 230, Kilifi, Kenya
    Parasite Immunol 28:51-60. 2006
  5. ncbi Serology: a robust indicator of malaria transmission intensity?
    Patrick Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine LSHTM, Keppel St, London, WC1E 7HT, UK
    Trends Parasitol 23:575-82. 2007
  6. pmc Dried blood spots as a source of anti-malarial antibodies for epidemiological studies
    Patrick H Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Malar J 7:195. 2008
  7. pmc Conditional expression of Toxoplasma gondii apical membrane antigen-1 (TgAMA1) demonstrates that TgAMA1 plays a critical role in host cell invasion
    Jeffrey Mital
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Mol Biol Cell 16:4341-9. 2005
  8. pmc Immunoglobulin G subclass-specific responses against Plasmodium falciparum merozoite antigens are associated with control of parasitemia and protection from symptomatic illness
    Danielle I Stanisic
    Infection and Immunity Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Australia
    Infect Immun 77:1165-74. 2009
  9. pmc Parasite antigens on the infected red cell surface are targets for naturally acquired immunity to malaria
    P C Bull
    Kenya Medical Research Institute CRC, Kilifi Unit
    Nat Med 4:358-60. 1998
  10. ncbi Genetic diversity and multiple infections of Plasmodium vivax malaria in Western Thailand
    Liwang Cui
    Department of Entomology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Am J Trop Med Hyg 68:613-9. 2003

Research Grants

  1. IMMUNOBIOLOGY OF MALARIA GLYCOSYLPHOSPHATIDYLINOSITOL
    Louis Schofield; Fiscal Year: 2001
  2. A CHEMICALLY SYNTHETIC ANTI-TOXIC VACCINE AGAINST MALARI
    Louis Schofield; Fiscal Year: 2002
  3. Adherence of malaria-infected red cells
    Alan Cowman; Fiscal Year: 2009
  4. Global GAS Vaccine Based On The M-Protein
    Michael Good; Fiscal Year: 2008
  5. Translation Research in Plasmodium vivax
    Socrates Herrera; Fiscal Year: 2005
  6. Vaccine Induced Mucosal Protection Against TB Infection
    DANIEL HOFT; Fiscal Year: 2005
  7. Chemokines in pathogenesis of Experimental Arthritis
    SEEMA SINGH AHUJA; Fiscal Year: 2010
  8. HELPER T CELL SUBSETS IN LEISHMANIASIS
    Richard Locksley; Fiscal Year: 2005
  9. Translational Control in Plasmodium Sexual Development
    Liwang Cui; Fiscal Year: 2005
  10. Immune Pathways as Prerequisites for Adjuvants' Efficacy
    George Hui; Fiscal Year: 2004

Detail Information

Publications311 found, 100 shown here

  1. pmc Estimating medium- and long-term trends in malaria transmission by using serological markers of malaria exposure
    C J Drakeley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 102:5108-13. 2005
    ..Thus, serological tools allow us to detect variations in malaria transmission over time. Such tools will be invaluable for monitoring trends in malaria endemicity and the effectiveness of malaria control programs...
  2. pmc Improved diagnostic testing and malaria treatment practices in Zambia
    Davidson H Hamer
    Center for International Health and Development, Boston University School of Public Health, Boston, Mass 02118, USA
    JAMA 297:2227-31. 2007
    ....
  3. pmc Rapid diagnostic tests for malaria parasites
    Anthony Moody
    Department of Clinical Parasitology, Hospital for Tropical Diseases, University Collge Hospital, London, United Kingdom
    Clin Microbiol Rev 15:66-78. 2002
    ..Clinical studies allow effective comparisons between different formats, and the reality of nonmicroscopic diagnoses of malaria is considered...
  4. ncbi Immune effector mechanisms in malaria
    K Marsh
    KEMRI Centre for Geographic Medicine Research Coast CGMRC, PO Box 230, Kilifi, Kenya
    Parasite Immunol 28:51-60. 2006
    ....
  5. ncbi Serology: a robust indicator of malaria transmission intensity?
    Patrick Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine LSHTM, Keppel St, London, WC1E 7HT, UK
    Trends Parasitol 23:575-82. 2007
    ..Here we present a suggested methodology, highlight the advantages and shortcomings of serological measurements of malaria transmission and identify areas in which further work is desirable...
  6. pmc Dried blood spots as a source of anti-malarial antibodies for epidemiological studies
    Patrick H Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Malar J 7:195. 2008
    ..Although recommendations for the storage and analysis of small molecule analytes in blood spots exist, there are no published systematic studies of the stability of antibodies under different storage conditions...
  7. pmc Conditional expression of Toxoplasma gondii apical membrane antigen-1 (TgAMA1) demonstrates that TgAMA1 plays a critical role in host cell invasion
    Jeffrey Mital
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Mol Biol Cell 16:4341-9. 2005
    ..The data suggest a model in which attachment of the parasite to the host cell occurs in two distinct stages, the second of which requires TgAMA1 and is involved in regulating rhoptry secretion...
  8. pmc Immunoglobulin G subclass-specific responses against Plasmodium falciparum merozoite antigens are associated with control of parasitemia and protection from symptomatic illness
    Danielle I Stanisic
    Infection and Immunity Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Australia
    Infect Immun 77:1165-74. 2009
    ..However, no antibody response was significantly associated with protection from parasitization per se. Our findings have major implications for understanding human immunity and for malaria vaccine development and evaluation...
  9. pmc Parasite antigens on the infected red cell surface are targets for naturally acquired immunity to malaria
    P C Bull
    Kenya Medical Research Institute CRC, Kilifi Unit
    Nat Med 4:358-60. 1998
    ..The apparent selective pressure exerted by established anti-PfEMP1 antibodies on infecting parasites supports the idea that such responses provide variant-specific protection against disease...
  10. ncbi Genetic diversity and multiple infections of Plasmodium vivax malaria in Western Thailand
    Liwang Cui
    Department of Entomology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Am J Trop Med Hyg 68:613-9. 2003
    ..These results strongly indicate that P. vivax populations are highly diverse and multiple clonal infections are common in this malaria-hypoendemic region of Thailand...
  11. pmc An inhibitory antibody blocks interactions between components of the malarial invasion machinery
    Christine R Collins
    Division of Parasitology, National Institute for Medical Research, Mill Hill, London, United Kingdom
    PLoS Pathog 5:e1000273. 2009
    ..We propose that mAb 4G2 inhibits invasion by preventing PfAMA1 from interacting with other components of the invasion complex. Our findings should aid the rational design of subunit malaria vaccines based on PfAMA1...
  12. pmc Phase 1 trial of malaria transmission blocking vaccine candidates Pfs25 and Pvs25 formulated with montanide ISA 51
    Yimin Wu
    Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, Rockville, Maryland, United States of America
    PLoS ONE 3:e2636. 2008
    ..This single blinded, dose escalating, controlled Phase 1 study assessed the safety and immunogenicity of recombinant Pfs25 and Pvs25 formulated with Montanide ISA 51, a water-in-oil emulsion...
  13. ncbi CD8+ T cells are required for primary immunity in C57BL/6 mice following low-dose, intradermal challenge with Leishmania major
    Yasmine Belkaid
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, and Dermatology Branch, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 168:3992-4000. 2002
    ..The low dose, intradermal challenge model reveals that CD8(+) T cells play an essential role in both pathogenesis of and immunity to primary infection with L. major in the skin...
  14. ncbi Rapid diagnostic tests for malaria at sites of varying transmission intensity in Uganda
    Heidi Hopkins
    University of California, San Francisco, San Francisco, California, USA
    J Infect Dis 197:510-8. 2008
    ..In Africa, fever is often treated presumptively as malaria, resulting in misdiagnosis and the overuse of antimalarial drugs. Rapid diagnostic tests (RDTs) for malaria may allow improved fever management...
  15. ncbi Human antibodies to recombinant protein constructs of Plasmodium falciparum Apical Membrane Antigen 1 (AMA1) and their associations with protection from malaria
    Spencer D Polley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Vaccine 23:718-28. 2004
    ..Results encourage the development of an AMA1 vaccine based on the full-length ectodomain, and indicate that the function of human antibodies to allele-specific and conserved epitopes in AMA1 should be studied further...
  16. ncbi The circumsporozoite protein is an immunodominant protective antigen in irradiated sporozoites
    Kota Arun Kumar
    Michael Heidelberger Division of Immunology, Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    Nature 444:937-40. 2006
    ..Thus, although hundreds of other Plasmodium genes are expressed in sporozoites and EEFs, CS is a dominant protective antigen. Nevertheless, sterile immunity could be obtained by immunization of CS transgenics with three doses of IrSp...
  17. ncbi Plasmodium vivax merozoite surface protein PvMSP-3 beta is radically polymorphic through mutation and large insertions and deletions
    Julian C Rayner
    Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, MS F 36, 4770 Buford Highway, Atlanta, GA 30341, USA
    Infect Genet Evol 4:309-19. 2004
    ..The implications for PvMSP-3 beta function and vaccine development are discussed...
  18. ncbi IL-4 rapidly produced by V beta 4 V alpha 8 CD4+ T cells instructs Th2 development and susceptibility to Leishmania major in BALB/c mice
    P Launois
    World Health Organization Immunology Research and Training Center, University of Lausanne, Epalinges, Switzerland
    Immunity 6:541-9. 1997
    ..Thus, the IL-4 required for Th2 development and susceptibility to L. major is produced by a restricted population of V beta 4 V alpha 8 CD4+ T cells after cognate interaction with a single antigen from this complex organism...
  19. pmc Phase 1/2a study of the malaria vaccine candidate apical membrane antigen-1 (AMA-1) administered in adjuvant system AS01B or AS02A
    Michele D Spring
    United States Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 4:e5254. 2009
    ....
  20. ncbi Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes
    Sharon Yeoh
    Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Cell 131:1072-83. 2007
    ..Our findings reveal the presence in the malarial parasitophorous vacuole of a regulated, PfSUB1-mediated proteolytic processing event required for release of viable parasites from the host erythrocyte...
  21. ncbi Infectiousness in a cohort of brazilian dogs: why culling fails to control visceral leishmaniasis in areas of high transmission
    Orin Courtenay
    Ecology and Epidemiology Group, Department of Biological Sciences, University of Warwick, Coventry, United Kingdom
    J Infect Dis 186:1314-20. 2002
    ..Mathematical modeling suggests that culling programs fail because of high incidence of infection and infectiousness, the insensitivity of the diagnostic test to detect infectious dogs, and time delays between diagnosis and culling...
  22. pmc Cohort study of the association of antibody levels to AMA1, MSP119, MSP3 and GLURP with protection from clinical malaria in Ghanaian children
    Daniel Dodoo
    Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
    Malar J 7:142. 2008
    ..This study was aimed at assessing in a standardized manner the relationship between the antibody responses to four malaria vaccine candidate antigens and protection from clinical malaria, in a cohort of Ghanaian children...
  23. ncbi A receptor for the malarial parasite Plasmodium vivax: the erythrocyte chemokine receptor
    R Horuk
    Genentech, Inc, South San Francisco, CA 94080
    Science 261:1182-4. 1993
    ..Third, both MGSA and IL-8 blocked the binding of the parasite ligand and the invasion of human erythrocytes by P. knowlesi, suggesting the possibility of receptor blockade for anti-malarial therapy...
  24. ncbi Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes
    Jean Loup Lemesre
    Institut de Recherche pour le Developpement, UR 008 Pathogénie des Trypanosomatidae, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
    Vaccine 23:2825-40. 2005
    ..infantum-infected CM-DM were co-cultured with autologous lymphocytes. Overall, our results support the view that a LiESAp vaccine might be useful in a promising vaccination approach against natural L. infantum infection...
  25. pmc Erythrocyte-binding antigen 175 mediates invasion in Plasmodium falciparum utilizing sialic acid-dependent and -independent pathways
    Manoj T Duraisingh
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Proc Natl Acad Sci U S A 100:4796-801. 2003
    ..This finding suggests a model where a minimal affinity supplied by multiple ligand-receptor interactions is required for successful invasion and has implications for EBA-175 as a malaria vaccine candidate...
  26. pmc A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests
    Dionicia Gamboa
    Instituto de Medicina Tropical Alexander von Humboldt Universidad Peruana Cayetano Heredia, Lima, Peru
    PLoS ONE 5:e8091. 2010
    ..The selection of the most suitable malaria RDTs is critical to the success of the programmes...
  27. pmc Assessment of the prozone effect in malaria rapid diagnostic tests
    Philippe Gillet
    Department of Clinical Sciences, Institute of Tropical Medicine ITM, Nationalestraat 155, B 2000 Antwerp, Belgium
    Malar J 8:271. 2009
    ..In this study, a panel of malaria RDTs was challenged with clinical samples with P. falciparum hyperparasitaemia (> 5% infected red blood cells)...
  28. pmc A randomized controlled phase 2 trial of the blood stage AMA1-C1/Alhydrogel malaria vaccine in children in Mali
    Issaka Sagara
    Malaria Research and Training Center, Faculty of Medicine Pharmacy and Dentistry BP 1805 Bamako, University of Bamako, Mali, Malaysia
    Vaccine 27:3090-8. 2009
    ..5 g/dL, in the direction of lower hemoglobin in the children who received AMA1-C1, although these differences were not significant after correction for multiple tests. These differences were not seen in the second year of transmission...
  29. pmc Variation in use of erythrocyte invasion pathways by Plasmodium falciparum mediates evasion of human inhibitory antibodies
    Kristina E M Persson
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    J Clin Invest 118:342-51. 2008
    ..falciparum and that a broad inhibitory response against multiple ligands may be required for effective immunity...
  30. ncbi Classification of adhesive domains in the Plasmodium falciparum erythrocyte membrane protein 1 family
    J D Smith
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Mol Biochem Parasitol 110:293-310. 2000
    ..This information might be used to develop interventions targeting parasite binding variants that cause disease...
  31. ncbi Antibodies to the conserved C-terminal domain of the Plasmodium falciparum merozoite surface protein 1 and to the merozoite extract and their relationship with in vitro inhibitory antibodies and protection against clinical malaria in a Senegalese village
    Ronald Perraut
    Unite d immunologie, Institut Pasteur de Dakar, 36 Ave Pasteur, BP 220, Dakar, Senegal
    J Infect Dis 191:264-71. 2005
    ..High levels of anti-PfMSP-1p19 immunoglobulin G were associated with reduced malaria in an age-adjusted multivariate analysis. These results support baculovirus PfMSP-1p19-based vaccine development...
  32. pmc Structure of AMA1 from Plasmodium falciparum reveals a clustering of polymorphisms that surround a conserved hydrophobic pocket
    Tao Bai
    University of Maryland School of Pharmacy, 20 Penn Street, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 102:12736-41. 2005
    ..Precedents set by viral receptor proteins would suggest that this is likely to be the AMA1 receptor binding pocket...
  33. ncbi P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1
    J A Rowe
    Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA
    Nature 388:292-5. 1997
    ..Thus we describe a new adhesive function for PfEMP1 and raise the possibility that CR1 polymorphisms in Africans that influence the interaction between erythrocytes and PfEMP1 may protect against severe malaria...
  34. pmc Induction of adhesion-inhibitory antibodies against placental Plasmodium falciparum parasites by using single domains of VAR2CSA
    Morten A Nielsen
    Department of International Health, Immunology, and Microbiology, University of Copenhagen, Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark
    Infect Immun 77:2482-7. 2009
    ..As the inhibition was not limited to homologous parasite strains, it seems feasible to base a protective malaria vaccine on a single VAR2CSA DBL domain...
  35. pmc A diversity-covering approach to immunization with Plasmodium falciparum apical membrane antigen 1 induces broader allelic recognition and growth inhibition responses in rabbits
    Edmond J Remarque
    Department of Parasitology, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands
    Infect Immun 76:2660-70. 2008
    ..This suggests that vaccines based upon the DiCo mix approach provide a broader functional immunity than immunization with a single allele...
  36. ncbi Plasmodium vivax merozoite surface protein-3 contains coiled-coil motifs in an alanine-rich central domain
    M R Galinski
    Department of Medical and Molecular Parasitology, New York University School of Medicine, NY 10010, USA
    Mol Biochem Parasitol 101:131-47. 1999
    ..PvMSP-3 is structurally related to P. falciparum MSP-3 and the 140000 Da MSP of P. knowlesi. Characterization of PvMSP-3, thus, also begins to define a new interspecies family of evolutionarily related Plasmodium merozoite proteins...
  37. pmc Update on rapid diagnostic testing for malaria
    Clinton K Murray
    Infectious Disease Service, Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, USA
    Clin Microbiol Rev 21:97-110. 2008
    ..This review highlights RDTs, including challenges in assessing their performance, internationally available RDTs, their effectiveness in various health care settings, and the selection of RDTs for different health care systems...
  38. ncbi Placental Plasmodium falciparum infection: causes and consequences of in utero sensitization to parasite antigens
    Kelly Broen
    Department of Medical Microbiology 268, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Mol Biochem Parasitol 151:1-8. 2007
    ....
  39. ncbi Plasmodium falciparum variant surface antigen expression varies between isolates causing severe and nonsevere malaria and is modified by acquired immunity
    Morten A Nielsen
    Center for Medical Parasitology, Rigshospitalet and University of Copenhagen, Copenhagen, Denmark
    J Immunol 168:3444-50. 2002
    ..This opens the possibility of developing morbidity-reducing vaccines targeting a limited subset of common and particularly virulent VSA...
  40. pmc Breadth and magnitude of antibody responses to multiple Plasmodium falciparum merozoite antigens are associated with protection from clinical malaria
    Faith H A Osier
    KEMRI Centre for Geographic Medicine Research, Coast, P O Box 230 80108, Kilifi, Kenya
    Infect Immun 76:2240-8. 2008
    ....
  41. pmc Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasion
    Sebastien Besteiro
    UMR 5235 CNRS, Université de Montpellier 2, Montpelier, France
    PLoS Pathog 5:e1000309. 2009
    ..Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes...
  42. pmc Antibody recognition of Plasmodium falciparum erythrocyte surface antigens in Kenya: evidence for rare and prevalent variants
    P C Bull
    Kenya Medical Research Institute CGMRC, Kilifi Unit, Kilifi, Kenya
    Infect Immun 67:733-9. 1999
    ..These results support the idea that preexisting anti-PfEMP1 antibodies can select the variants that are expressed during a new infection and may suggest the existence of a dominant subset of PfEMP1 variants...
  43. ncbi VAR2CSA is the principal ligand for chondroitin sulfate A in two allogeneic isolates of Plasmodium falciparum
    Michael F Duffy
    The Department of Medicine, The University of Melbourne, The Royal Melbourne Hospital, Parkville, VIC 3050, Australia
    Mol Biochem Parasitol 148:117-24. 2006
    ....
  44. ncbi Transient cross-reactive immune responses can orchestrate antigenic variation in malaria
    Mario Recker
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Nature 429:555-8. 2004
    ..Antigenic variation has always been seen as an adaptation of the parasite to evade host defence: we show that the coordination necessary for the success of this strategy might be provided by the host...
  45. ncbi Ensuring quality and access for malaria diagnosis: how can it be achieved?
    David Bell
    Malaria, other Vector borne and Parasitic Diseases, World Health Organization Regional Office for the Western Pacific, P O Box 2932, Manila, Philippines
    Nat Rev Microbiol 4:682-95. 2006
    ....
  46. ncbi Phase 1 vaccine trial of Pvs25H: a transmission blocking vaccine for Plasmodium vivax malaria
    Elissa M Malkin
    Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Twinbrook 1, Room 1123, Rockville, MD 20852, USA
    Vaccine 23:3131-8. 2005
    ..Pvs25H generates transmission blocking immunity in humans against P. vivax demonstrating the potential of this antigen as a component of a transmission blocking vaccine...
  47. pmc Polymorphism in the Plasmodium falciparum erythrocyte-binding ligand JESEBL/EBA-181 alters its receptor specificity
    D C Ghislaine Mayer
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, Room 4126, Bethesda, MD 20892 0425, USA
    Proc Natl Acad Sci U S A 101:2518-23. 2004
    ..falciparum may have been successful in endemic areas because it has mutated the ligands of the DBL family to create multiple pathways of invasion, thus making selection of refractory erythrocytes unlikely...
  48. pmc PfEMP1-DBL1alpha amino acid motifs in severe disease states of Plasmodium falciparum malaria
    Johan Normark
    Department of Microbiology and Tumor and Cell Biology MTC, Karolinska Institutet and Swedish Institute for Infectious Diseases Control SMI, Box 280, Nobels vag 16, SE 17177 Stockholm, Sweden
    Proc Natl Acad Sci U S A 104:15835-40. 2007
    ..The method is potentially applicable to other families of variant proteins and may be useful in identifying sequence-phenotype relationships. The results suggest that certain PfEMP1 sequences are predisposed to inducing severe malaria...
  49. ncbi High sequence diversity and evidence of balancing selection in the Pvmsp3alpha gene of Plasmodium vivax in the Venezuelan Amazon
    Rosalynn Ord
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Mol Biochem Parasitol 144:86-93. 2005
    ..Our findings support the use of Pvmsp3alpha genotyping as a tool for monitoring interventions aimed at control of P. vivax...
  50. ncbi Correlation of high levels of antibodies to multiple pre-erythrocytic Plasmodium falciparum antigens and protection from infection
    Chandy C John
    Center for Global Health and Diseases, Rainbow Center for International Child Health, Division of Pediatric Infectious Diseases, Case Western Reserve University, Cleveland, Ohio, USA
    Am J Trop Med Hyg 73:222-8. 2005
    ..High levels of IgG antibodies to CSP, LSA-1, and TRAP may be useful immune correlates of protection against P. falciparum infection in malaria-endemic populations...
  51. ncbi Immunogenicity and protective efficacy of Escherichia coli expressed Plasmodium falciparum merozoite surface protein-1(42) using human compatible adjuvants
    Suraksha Sachdeva
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
    Vaccine 24:2007-16. 2006
    ..berghei/P. falciparum chimeric line (Pb-PfM19) that expresses Plasmodium falciparum MSP-1(19). These findings may be useful for the development of a malaria vaccine based on Plasmodium falciparum MSP-1(42)...
  52. ncbi Parasite ligand-host receptor interactions during invasion of erythrocytes by Plasmodium merozoites
    Deepak Gaur
    Laboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Building Twinbrook III Room 3E 32D, Bethesda, MD 20892 8132, USA
    Int J Parasitol 34:1413-29. 2004
    ..Here we review the current state of knowledge about the receptor-ligand interactions that mediate merozoite invasion of erythrocytes...
  53. ncbi Molecular evolution and intragenic recombination of the merozoite surface protein MSP-3alpha from the malaria parasite Plasmodium vivax in Thailand
    C N Mascorro
    Department of Entomology, The Pennsylvania State University, 501 ASI Building, University Park, PA 16802, USA
    Parasitology 131:25-35. 2005
    ..The highly polymorphic nature and lack of geographical clustering of isolates suggest that more systematic investigations of the PvMSP-3alpha gene are needed to explore its evolution and vaccine potential...
  54. ncbi Sulphated tyrosines mediate association of chemokines and Plasmodium vivax Duffy binding protein with the Duffy antigen/receptor for chemokines (DARC)
    Hyeryun Choe
    Perlmutter Laboratory, Children s Hospital, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 55:1413-22. 2005
    ..These data are consistent with a role for tyrosine sulphation in the association of many or most chemokines with their receptors, and identify a key molecular determinant of erythrocyte invasion by P. vivax...
  55. ncbi Crystal structure of the malaria vaccine candidate apical membrane antigen 1
    Juan Carlos Pizarro
    Unité d Immunologie Structurale, Centre National de la Recherche Scientifique, URA 2185, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, France
    Science 308:408-11. 2005
    ..These results are likely to aid vaccine and drug design...
  56. pmc Conserved residues in the Plasmodium vivax Duffy-binding protein ligand domain are critical for erythrocyte receptor recognition
    Kelley M VanBuskirk
    Department of Biological Sciences, Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, IN 46556, USA
    Proc Natl Acad Sci U S A 101:15754-9. 2004
    ..These data delineate DBL domain residues essential for receptor recognition...
  57. pmc Persistent histidine-rich protein 2, parasite lactate dehydrogenase, and panmalarial antigen reactivity after clearance of Plasmodium falciparum monoinfection
    Jamshaid Iqbal
    Department of Microbiology, Faculty of Medicine, Kuwait University, P O Box 24923, Safat 13110, Kuwait
    J Clin Microbiol 42:4237-41. 2004
    ..In conclusion, the OptiMAL-IT assay is more sensitive than the ICT Malaria Pf/Pv test for monitoring therapeutic responses after antimalarial therapy since the LDH activity ceases when the malarial parasite dies...
  58. ncbi Selective upregulation of a single distinctly structured var gene in chondroitin sulphate A-adhering Plasmodium falciparum involved in pregnancy-associated malaria
    Ali Salanti
    Centre for Medical Parasitology at the Institute for Medical Microbiology and Immunology, University of Copenhagen, Panum Institute 24 2, and Department of Infectious Diseases, Copenhagen University Hospital Rigshospitalet, Denmark
    Mol Microbiol 49:179-91. 2003
    ..falciparum malaria. This var gene thus has the properties expected of a gene encoding the parasite adhesion molecule that initiates the pathology associated with PAM...
  59. ncbi The genetic diversity of Plasmodium vivax populations
    Liwang Cui
    Department of Entomology, Penn State University, 501 ASI, University Park, PA 16802, USA
    Trends Parasitol 19:220-6. 2003
    ..This article reviews the known polymorphic genetic markers, summarizes current data on the population structure of this parasite and discusses future prospects for using knowledge of the genetic diversity to improve control measures...
  60. ncbi Protective vaccination against experimental canine visceral leishmaniasis using a combination of DNA and protein immunization with cysteine proteinases type I and II of L. infantum
    Sima Rafati
    Molecular Immunology Lab, Department of Immunology, Pasteur Institute of Iran, P O Box 11365 6699, Tehran, Iran
    Vaccine 23:3716-25. 2005
    ..We propose that the combination of DNA and recombinant protein vaccination using CPs could be instrumental to control (VL) in dogs...
  61. pmc Plasmodium vivax: who cares?
    Mary R Galinski
    Emory Vaccine Center and Yerkes National Primate Research Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University, Atlanta, GA, USA
    Malar J 7:S9. 2008
    ....
  62. pmc Trafficking of STEVOR to the Maurer's clefts in Plasmodium falciparum-infected erythrocytes
    Jude M Przyborski
    Hygiene Institut, Abteilung Parasitologie, Universitatsklinikum Heidelberg, Heidelberg, Germany
    EMBO J 24:2306-17. 2005
    ..Our data suggest general principles for transport of transmembrane proteins to the Maurer's clefts and provide new insights into protein sorting and trafficking processes in P. falciparum...
  63. ncbi A human phase 1 vaccine clinical trial of the Plasmodium falciparum malaria vaccine candidate apical membrane antigen 1 in Montanide ISA720 adjuvant
    Allan Saul
    The Cooperative Research Centre for Vaccine Technology, Brisbane, QLD, Australia
    Vaccine 23:3076-83. 2005
    ..Six of the 29 subjects seroconverted. Only one of these developed a high antibody titre. However, the interpretation of this trial was compromised by a loss of potency of the formulated vaccine during the course of the study...
  64. ncbi Longitudinal study of natural immune responses to the Plasmodium falciparum apical membrane antigen (AMA-1) in a holoendemic region of malaria in western Kenya: Asembo Bay Cohort Project VIII
    V Udhayakumar
    Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Atlanta, Georgia, USA
    Am J Trop Med Hyg 65:100-7. 2001
    ..009). Responses to AMA-1 T-cell epitopes were short lived, and antibody responses were relatively stable...
  65. ncbi Comparison of PCR-RFLP and Genescan-based genotyping for analyzing infection dynamics of Plasmodium falciparum
    Nicole Falk
    Swiss Tropical Institute, Basel, Switzerland
    Am J Trop Med Hyg 74:944-50. 2006
    ..This reflects the good performance of GeneScan-based genotyping for studies of parasite infection dynamics...
  66. pmc Evidence for globally shared, cross-reacting polymorphic epitopes in the pregnancy-associated malaria vaccine candidate VAR2CSA
    Marion Avril
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA 98109 5219, USA
    Infect Immun 76:1791-800. 2008
    ..This study demonstrates that VAR2CSA contains common polymorphic epitopes that are shared between geographically diverse CSA-binding lines...
  67. pmc Malaria-induced acquisition of antibodies to Plasmodium falciparum variant surface antigens
    Michael F Ofori
    Immunology and Epidemiology Units, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
    Infect Immun 70:2982-8. 2002
    ..The data from this first detailed longitudinal study of acquisition of VSA antibodies support the hypothesis that naturally acquired protective immunity to P. falciparum malaria is mediated, at least in part, by VSA-specific antibodies...
  68. pmc Functional analysis of the leading malaria vaccine candidate AMA-1 reveals an essential role for the cytoplasmic domain in the invasion process
    Moritz Treeck
    Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS Pathog 5:e1000322. 2009
    ..We show that the cytoplasmic domain of AMA-1 is phosphorylated. Mutational analysis suggests an important role for the phosphorylation in the invasion process, which might translate into novel therapeutic strategies...
  69. ncbi A subset of Plasmodium falciparum SERA genes are expressed and appear to play an important role in the erythrocytic cycle
    Susanne K Miller
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    J Biol Chem 277:47524-32. 2002
    ..The inability to disrupt SERA4, -5, and -6 is consistent with their apparent dominant expression and implies an important role for these genes in maintenance of the erythrocytic cycle...
  70. pmc Operational accuracy and comparative persistent antigenicity of HRP2 rapid diagnostic tests for Plasmodium falciparum malaria in a hyperendemic region of Uganda
    Daniel J Kyabayinze
    Malaria Consortium Africa, Plot 2, Sturrock Road, P O Box 8045, Kampala, Uganda
    Malar J 7:221. 2008
    ..An assessment of the accuracy of Malaria Pf immuno-chromatographic test (ICT) and description of persistent antigenicity of HRP2 RDTs was undertaken in a hyperendemic area of Uganda...
  71. ncbi Immune response to pre-erythrocytic stages of malaria parasites
    D L Doolan
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
    Curr Mol Med 6:169-85. 2006
    ..Herein, we review our current understanding of the molecular and cellular aspects of pre-erythrocytic stage immunity...
  72. ncbi Making malaria testing relevant: beyond test purchase
    David Bell
    Malaria, other Vector borne and Parasitic Diseases, World Health Organization, Regional Office for the Western Pacific, UN Avenue, Ermita, P O Box 2932, Manila, The Philippines
    Trans R Soc Trop Med Hyg 102:1064-6. 2008
    ..If this is to occur, a re-think is required of the way many malaria programmes are funded and run...
  73. ncbi VAR2CSA domains expressed in Escherichia coli induce cross-reactive antibodies to native protein
    Andrew V Oleinikov
    Seattle Biomedical Research Institute, University of Washington, Seattle, USA
    J Infect Dis 197:1119-23. 2008
    ..This is the first report that E. coli-expressed VAR2CSA domains induce antibody to native VAR2CSA...
  74. pmc The structure of a chondroitin sulfate-binding domain important in placental malaria
    Matthew K Higgins
    Department of Biochemistry, University of Cambridge, 80, Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 283:21842-6. 2008
    ..This suggests a model for how the domain maintains ligand binding while evading the immune response and will guide future drug and vaccine development...
  75. ncbi The SRS superfamily of Toxoplasma surface proteins
    Calvin Jung
    Infectious Diseases, Department of Medicine, University of British Columbia, D459 HP East, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5
    Int J Parasitol 34:285-96. 2004
    ..Orthologous genes are restricted to tissue-dwelling coccidia (Neospora, Sarcocystis) with no related sequences present in other more distant apicomplexa such as Eimeria, Cryptosporidia, and Plasmodium spp...
  76. pmc Identification of the moving junction complex of Toxoplasma gondii: a collaboration between distinct secretory organelles
    David L Alexander
    Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
    PLoS Pathog 1:e17. 2005
    ..Differences between the contributing proteins in different species may, in part, be the result of selective pressure from the different niches occupied by these parasites...
  77. pmc Binding hot spot for invasion inhibitory molecules on Plasmodium falciparum apical membrane antigen 1
    Karen S Harris
    Department of Biochemistry, La Trobe University, Victoria, Australia
    Infect Immun 73:6981-9. 2005
    ..The interaction between these peptides and AMA1 may further our understanding of the molecular mechanisms of invasion by identifying critical functional regions of AMA1 and aid in the development of novel antimalarial strategies...
  78. pmc Repeat sequences in block 2 of Plasmodium falciparum merozoite surface protein 1 are targets of antibodies associated with protection from malaria
    Spencer D Polley
    London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Infect Immun 71:1833-42. 2003
    ..The results are important for design of a vaccine to induce protective antibodies, and they address hypotheses about repeat sequences in malaria antigens...
  79. ncbi The sickle cell trait is associated with enhanced immunoglobulin G antibody responses to Plasmodium falciparum variant surface antigens
    Gerardo Cabrera
    Department of Parasitology, Institute for Tropical Medicine, University of Tubingen, Tubingen, Germany
    J Infect Dis 191:1631-8. 2005
    ..The results imply that enhanced levels of cross-reactive anti-VSA responses in children with HbAS may be intimately associated with the protection they have against malaria...
  80. pmc Merozoite surface protein-3alpha is a reliable marker for population genetic analysis of Plasmodium vivax
    Sedigheh Zakeri
    Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
    Malar J 5:53. 2006
    ..In this study, msp-3alpha was compared and assessed with msp-1 marker in order to find whether msp-3alpha is a reliable genetic marker for P. vivax population studies...
  81. ncbi Importance of CD8(+)Vbeta8(+) T cells in IFN-gamma-mediated prevention of toxoplasmic encephalitis in genetically resistant BALB/c mice
    Xisheng Wang
    Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    J Interferon Cytokine Res 25:338-44. 2005
    ....
  82. ncbi Merozoite surface protein-9 of Plasmodium vivax and related simian malaria parasites is orthologous to p101/ABRA of P. falciparum
    Esmeralda Vargas-Serrato
    Department of Medicine, Emory Vaccine Research Center, Yerkes Primate Research Center, Emory University, 954 Gatewood Rd, Atlanta, GA 30329, USA
    Mol Biochem Parasitol 120:41-52. 2002
    ..Comparative interspecies investigations of the potential role(s) of Plasmodium MSP-9 in merozoite invasion of erythrocytes and as a malaria vaccine candidate can now be pursued...
  83. pmc Sub-grouping of Plasmodium falciparum 3D7 var genes based on sequence analysis of coding and non-coding regions
    Thomas Lavstsen
    Centre for Medical Parasitology at Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark
    Malar J 2:27. 2003
    ..The sequence diversity and organization of the 3D7 PfEMP1 repertoire was investigated on the basis of the complete genome sequence...
  84. pmc Long-term clinical protection from falciparum malaria is strongly associated with IgG3 antibodies to merozoite surface protein 3
    Christian Roussilhon
    Bio medical Parasitology Unit, Institut Pasteur, Paris, France
    PLoS Med 4:e320. 2007
    ..e., induced by the parasite) to molecules that are considered as valuable vaccine candidates...
  85. ncbi Canine visceral leishmaniasis in Colombia: relationship between clinical and parasitologic status and infectivity for sand flies
    B L Travi
    , Cali, Colombia
    Am J Trop Med Hyg 64:119-24. 2001
    ..The infectivity of dogs from clinically comparable groups from Colombian and Mediterranean foci differed. This may be a reflection of varied nutritional conditions or vector competency of distinct sand fly species...
  86. ncbi [No influence of season of transmission nor age of patients on the complexity and genetic diversity of Plasmodium falciparum infection in Cotonou, Benin]
    S Issifou
    , , , 04 B.P 0320, Cotonou,
    Bull Soc Pathol Exot 94:195-8. 2001
    ..Data obtained here will serve as a baseline for future studies, such as the impact of malaria control measures on parasite populations, to be conducted in Cotonou...
  87. ncbi Are trials in New World monkeys on the critical path for blood-stage malaria vaccine development?
    A W Stowers
    Malaria Vaccine Development Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infections Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Trends Parasitol 17:415-9. 2001
    ..Here, we argue that first proving efficacy in the New World monkey challenge model would accelerate development...
  88. pmc Characterization of a Plasmodium falciparum erythrocyte-binding protein paralogous to EBA-175
    D C Mayer
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, Room 4/126, Bethesda, MD 20892-0425, USA
    Proc Natl Acad Sci U S A 98:5222-7. 2001
    ..The interest in BAEBL's reduced binding to Gerbich erythrocytes derives from the high frequency of the Gerbich phenotype in some regions of Papua New Guinea where P. falciparum is hyperendemic...
  89. ncbi Antibody responses to a panel of Plasmodium falciparum malaria blood-stage antigens in relation to clinical disease outcome in Sudan
    Nnaemeka C Iriemenam
    Department of Immunology, Wenner Gren Institute, Stockholm University, SE 106 91 Stockholm, Sweden
    Vaccine 27:62-71. 2009
    ..The study provides further support for the potential importance of the studied merozoite vaccine candidate antigens as targets for parasite neutralizing antibody responses of the IgG1 and IgG3 subclasses...
  90. pmc Active transcription is required for maintenance of epigenetic memory in the malaria parasite Plasmodium falciparum
    Ron Dzikowski
    Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, The Institute for Medical Research, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Mol Biol 382:288-97. 2008
    ....
  91. ncbi Antigenic variation in Babesia bovis occurs through segmental gene conversion of the ves multigene family, within a bidirectional locus of active transcription
    Basima Al-Khedery
    Department of Pathobiology, University of Florida, Gainesville, 32611, USA
    Mol Microbiol 59:402-14. 2006
    ..These results also suggest the possibility of epigenetic regulation through in situ switching among gene loci, further expanding the potential repertoire of variant proteins...
  92. ncbi The role of CpG ODN in enhancement of immune response and protection in BALB/c mice immunized with recombinant major surface glycoprotein of Leishmania (rgp63) encapsulated in cationic liposome
    Mahmoud R Jaafari
    School of Pharmacy, Biotechnology Research Center and Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
    Vaccine 25:6107-17. 2007
    ..The results indicate that co-encapsulation of CpG ODN in liposomes improves the immunogenicity of Leishmania antigen...
  93. ncbi The carboxy-terminus of merozoite surface protein 1: structure, specific antibodies and immunity to malaria
    A A Holder
    Division of Parasitology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London
    Parasitology 136:1445-56. 2009
    ..Understanding the structure of MSP1 will help us design and engineer better antigens that are more effective than the first generation of vaccine candidates. This review is focused on the carboxy-terminus of MSP1...
  94. ncbi A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type 1 patients and a prognostic indicator for monitoring patients undergoing drug therapy
    R L Houghton
    Corixa Corp, Seattle, Washington 98104, USA
    J Infect Dis 177:1339-44. 1998
    ..In contrast, most patients who clinically relapsed showed increased antibody titers to rK39. These data demonstrate the diagnostic and prognostic utility of rK39 in detecting active visceral leishmaniasis...
  95. ncbi Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1
    E N Kimbita
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
    Vet Parasitol 102:35-44. 2001
    ..The results of ELISA were in agreement with those of a commercially available latex agglutination test (LAT) kit, although the former had higher titers than the latter...
  96. pmc Immunization with H1, HASPB1 and MML Leishmania proteins in a vaccine trial against experimental canine leishmaniasis
    J Moreno
    WHO Collaborating Centre for Leishmaniasis, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Spain
    Vaccine 25:5290-300. 2007
    ..The results demonstrate that HASPB1 and H1 antigens in combination with Montanide were able to induce partial protection against canine leishmaniasis, even under extreme experimental challenge conditions...
  97. ncbi Heterogeneity, geographic distribution, and pathogenicity of serodemes of Leishmania viannia in Colombia
    Nancy Gore Saravia
    Centro Internacional de Entrenamiento e Investigaciones Medicas, Cali, Colombia
    Am J Trop Med Hyg 66:738-44. 2002
    ..panamensis strains. Null strains of L. braziliensis and L. panamensis were more frequently isolated from mucosal leishmaniasis than strains that expressed species specific epitopes, suggesting that these strains may be more pathogenic...
  98. pmc Structure of the DBL3x domain of pregnancy-associated malaria protein VAR2CSA complexed with chondroitin sulfate A
    Kavita Singh
    Structural Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, Maryland 20852, USA
    Nat Struct Mol Biol 15:932-8. 2008
    ..The location of the CSA binding site is an important step forward in the molecular understanding of pregnancy-associated malaria and offers a new target for vaccine development...
  99. ncbi Structural comparison of apical membrane antigen 1 orthologues and paralogues in apicomplexan parasites
    Marie Laure Chesne-Seck
    Unité d Immunologie Structurale, CNRS URA 2185, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, France
    Mol Biochem Parasitol 144:55-67. 2005
    ....
  100. pmc The importance of human FcgammaRI in mediating protection to malaria
    Richard S McIntosh
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, United Kingdom
    PLoS Pathog 3:e72. 2007
    ..This important finding documents the capacity of FcgammaRI to mediate potent antimalaria immunity and supports the development of FcgammaRI-directed therapy for human malaria...
  101. ncbi Infection with Trypanosoma cruzi restricts the repertoire of parasite-specific CD8+ T cells leading to immunodominance
    Fanny Tzelepis
    Centro Interdisciplinar de Terapia Gênica and Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Mirasol 207, Sao Paulo, Brazil
    J Immunol 180:1737-48. 2008
    ....

Research Grants69

  1. IMMUNOBIOLOGY OF MALARIA GLYCOSYLPHOSPHATIDYLINOSITOL
    Louis Schofield; Fiscal Year: 2001
    ..These areas are central to understanding the host/parasite interaction and the development of effective vaccines to several protozoal pathogens. ..
  2. A CHEMICALLY SYNTHETIC ANTI-TOXIC VACCINE AGAINST MALARI
    Louis Schofield; Fiscal Year: 2002
    ..Clinical immunity to malaria is acquired considerably earlier than anti-parasite immunity, and it is possible that this is mediated by anti-toxin mechanisms. ..
  3. Adherence of malaria-infected red cells
    Alan Cowman; Fiscal Year: 2009
    ..These studies will contribute to and increased understanding of the pathophysiology of falciparum malaria. ..
  4. Global GAS Vaccine Based On The M-Protein
    Michael Good; Fiscal Year: 2008
    ..abstract_text> ..
  5. Translation Research in Plasmodium vivax
    Socrates Herrera; Fiscal Year: 2005
    ..abstract_text> ..
  6. Vaccine Induced Mucosal Protection Against TB Infection
    DANIEL HOFT; Fiscal Year: 2005
    ..Prime/boosting strategies found to prevent BCG infection after aerosol challenges will be studied for the ability to prevent infection with virulent M. tuberculosis by aerosol challenge. ..
  7. Chemokines in pathogenesis of Experimental Arthritis
    SEEMA SINGH AHUJA; Fiscal Year: 2010
    ..Thus information obtained from proposed studies which explore how CCR2 modulates arthritis will have significant impact on potential therapeutic strategies to treat this chronic deblitating disease. ..
  8. HELPER T CELL SUBSETS IN LEISHMANIASIS
    Richard Locksley; Fiscal Year: 2005
    ..The use of a parasite model in which the outcome has been so clearly tied to this decision offers an opportunity for important insights into disease susceptibility and effective vaccine strategy. ..
  9. Translational Control in Plasmodium Sexual Development
    Liwang Cui; Fiscal Year: 2005
    ....
  10. Immune Pathways as Prerequisites for Adjuvants' Efficacy
    George Hui; Fiscal Year: 2004
    ....
  11. MOLECULAR ANALYSIS OF ANTIGENIC VARIATION IN MALARIA
    Mary Galinski; Fiscal Year: 2004
    ....
  12. A new family of secreted adhesive proteins in Plasmodium
    THOMAS TEMPLETON; Fiscal Year: 2008
    ....
  13. Generation of babesial surface protein diversity
    SHAWN BERENS; Fiscal Year: 2006
    ..The sponsors collaborate closely in research and have successfully mentored clinicians, graduate students, and post-doctoral fellows to research independence. ..
  14. Utility of rapid diagnostic tests for malaria
    Heidi Hopkins; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  15. MOLECULAR TOOLS TO DEVELOP HAMSTER MODELS OF DISEASE
    Peter Melby; Fiscal Year: 2003
    ..This work will have particular relevance to research traditionally funded through a number of NIH institutes including the NCI, NIAID, NHLBI, NIA, NIAMS, and NIDR. ..
  16. VACCINATION OF DOGS TO REDUCE TRANSMISSION OF LEISHMANIA
    Peter Melby; Fiscal Year: 2003
    ..Successful vaccination of dogs in an endemic could reduce both enzootic (among the canine reservoir) and zoonotic (from the canine reservoir to humans) transmission. ..
  17. Development of a cell-based method for high-throughput drug screening in malaria
    Liwang Cui; Fiscal Year: 2008
    ..To facilitate antimalarial drug discovery, we propose to develop a highly sensitive, cell-based, one-step luminescent drug screen assay for high throughput screening of large chemical libraries. [unreadable] [unreadable] [unreadable]..
  18. NOS2 and arginase in visceral leishmaniasis
    Peter Melby; Fiscal Year: 2009
    ..These studies in the hamster will fill a knowledge gap by defining mechanisms of disease and immunity that are strikingly different from those of the murine model. ..
  19. ZEISS AXIOPLAN 2 IMAGING SYSTEM
    ROBERTA O CONNOR; Fiscal Year: 2005
    ..abstract_text> ..
  20. TRYPANOSOMA CRUZI MUCOSAL & SYSTEMIC PROTECTIVE IMMUNITY
    DANIEL HOFT; Fiscal Year: 2008
    ..Chemokine and/or integrin receptor expression will be studied by flow cytometry and RT-Real-Time PCR, and the in vivo effects of defective chemokine and integrin binding and/or signaling will be evaluated. [unreadable] [unreadable]..
  21. Chemokine System in DC and Lymphocyte Function
    SEEMA AHUJA; Fiscal Year: 2005
    ....
  22. Plasmodium vivax:Proteomic Analysis of Asexual and Sporozoite Stages
    Liwang Cui; Fiscal Year: 2007
    ..Its recent resurgence in prevalence and emergence of drug- resistant parasite strains have raised a great concern for the future control of malaria. [unreadable] [unreadable] [unreadable]..
  23. Role of Cryptosporidium Mucins in Host-parasite interactions
    ROBERTA O CONNOR; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  24. Gut Epithelial Cell Receptors for C parvum Invasion
    ROBERTA O CONNOR; Fiscal Year: 2005
    ..New England Medical Center, in association with Tufts University and the wider Boston scientific community provides researchers with diverse and excellent scientific resources, and is exceptional environment for a young scientist...
  25. Evaluation of High-Capacity Adenovectors in the Eye
    Joseph Bruder; Fiscal Year: 2006
    ..The long-term goal of this proposal is the clinical testing and commercialization of PEDF expressing HC vector for the treatment of AMD and other ocular neovascular diseases. [unreadable] [unreadable]..
  26. High Throughput Analysis of Malarial Surface Antigens
    ANDREW OLEINIKOV; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  27. Plasma Proteome During Pregnancy Malaria
    Michal Fried; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  28. Mucosal immune regulation in murine amebic colitis
    Eric Houpt; Fiscal Year: 2007
    ..The comprehensive approach in training and research laid out in this research proposal will guide the applicant towards his goal of being an independent investigator in immunoparasitology. ..
  29. A Therapeutic Target for Microsporidiosis
    Louis Weiss; Fiscal Year: 2007
    ..In addition, the approaches and methods developed in this R21 should also prove useful for studies on methionine aminopeptidases as therapeutic targets in other pathogenic protists. [unreadable] [unreadable] [unreadable]..
  30. Functional Genomics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2007
    ..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
  31. Gamma-Delta T cells and Cryptosporidiosis
    Beth Kirkpatrick; Fiscal Year: 2008
    ..These data will advance our understanding of the mucosal immune response to cryptosporidiosis, which will lead to further advances in prevention and therapy. ..
  32. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2010
    ..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..
  33. Mouse Strain dependent innate resistance to intestinal amebiasis
    Eric Houpt; Fiscal Year: 2009
    ....
  34. Role of hookworm secretions in host immunomodulation
    Stephanie Constant; Fiscal Year: 2009
    ..In turn these have the potential to provide novel targets for therapeutic and/or preventative strategies for infections by hookworms, as well as other types of pathogens. ..
  35. TOXOPLASMA ENCEPHALITIS IN AIDS: BRADYZOITE REGULATION
    Louis Weiss; Fiscal Year: 2009
    ....
  36. Diverse Neutralizing Human Monoclonal Antibodies to CMV
    ANGRAY KANG; Fiscal Year: 2003
    ..The phase II SBIR proposal may initially require the leneration of a larger panel of antibodies, based on the success of phase I. ..
  37. GENETIC DISSECTION OF TOXOPLASMA GONDII
    David Roos; Fiscal Year: 2008
    ..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
  38. Characterization of a vaccine against Chlamydia trachomatis
    RHEA COLER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  39. DEVELOPMENT OF THE HBCAG AS A VACCINE CARRIER PLATFORM
    David Milich; Fiscal Year: 2008
    ..abstract_text> ..
  40. Confocal Microscope for Microbiology
    L Sibley; Fiscal Year: 2002
    ..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
  41. Regulation of the Pax-5 Proto-Oncogen by Redox Mec,
    James Hagman; Fiscal Year: 2005
    ..Our studies will eventually aid in devising new therapeutics for treating Pax-related cancers (lymphoma, astrocytoma, and rhabdomyosarcoma). ..
  42. VACCINE DEVELOPMENT IN TUBERCULOSIS
    Antonio Campos Neto; Fiscal Year: 2004
    ..In addition to the evaluation of these antigens, we will characterize and clone other vaccine candidates using recently developed approaches for the direct identification of both CD4 and CD8 T cell antigens. ..
  43. MOLECULAR GENETICS OF SINDBIS VIRUS REPLICATION
    Charles Rice; Fiscal Year: 2005
    ....
  44. The Role of Babesia bovis MSA-1 in Erythrocyte Invasion
    Tanya LeRoith; Fiscal Year: 2004
    ..The results of this project, as well as the transfection techniques that will be developed, will allow us to begin targeting regions of functional significance in these proteins for further study...
  45. Urinary Symptoms in Adolescent Females: STI or UTI?
    Jill Huppert; Fiscal Year: 2004
    ..The findings of this study will strengthen the evidence base for symptom-based STI testing of SA adolescent females and will promote care that addresses both individual patient and public health needs. ..
  46. Comparison of US and European Babesia divergens Isolates
    Patricia Holman; Fiscal Year: 2004
    ..The molecular aspects may provide information that will be useful in future efforts to devise diagnostic tests. ..
  47. Species-specific diagnosis of human malaria
    Michael Makler; Fiscal Year: 2003
    ..The work described here will allow the development of a new test prototype that embodies these needed improvements. ..
  48. ROLE OF DUFFY GLYCOPROTEIN IN MURINE MALARIA INFECTIONS
    Christine Olver; Fiscal Year: 2002
    ..yoelii susceptibility, as is seen in a congenic mouse model. The data generated from this proposal will be used to publish three manuscripts, and will form the basis for an R01 to be submitted in the third or fourth year of funding. ..
  49. BIOLOGICAL VARIATION AMONG AFRICAN TRYPANOSOMES
    John Mansfield; Fiscal Year: 2002
    ..Second, the role of these genes in virulence would be confirmed by genetic manipulation of trypanosomes using gene complementation, disruption and over expression. ..
  50. GALACTOSE LECTIN OF E HISTOLYTICA
    Barbara Mann; Fiscal Year: 2002
    ..Successful completion of these experiments will provide insight into the role and identity of PTK/substrates in lectin-mediated killing and may establish the basis for novel therapeutic or prophylactic measures against amebiasis. ..
  51. Regulation of leukocyte trafficking by cyclophilins
    Stephanie Constant; Fiscal Year: 2005
    ..This will be examined using both in vitro and in vivo approaches, the latter using a mouse model of lung inflammation. ..
  52. Vaccine Development in Leishmaniasis
    Phillip Scott; Fiscal Year: 2006
    ..Overall, these experiments will establish the parameters for using LPG2 KO parasites as a vaccine in non-human primates, and provide basic information of wider importance in developing a leishmanial vaccine. [unreadable] [unreadable]..
  53. Real-time PCR diagnostic kit for Giardia and Cryptosporidium
    Eric Houpt; Fiscal Year: 2006
    ..A Phase II application is anticipated to validate the final diagnostic test product against immunoassay for an FDA 501 (k) medical device application. [unreadable] [unreadable] [unreadable]..
  54. HEPATIC ISCHEMIA/REPERFUSION-INDUCED LUNG INJURY
    ALEX LENTSCH; Fiscal Year: 2006
    ..The knowledge gained from these studies will provide mechanistic explanations for new therapeutic options for acute lung injury, and may be applicable to a number of other inflammatory lung diseases. ..
  55. Viruses And Cells Gordon Conference
    Charles Rice; Fiscal Year: 2005
    ..Funding is requested to support the conference fees for graduate students and postdoctoral fellows to this meeting. ..
  56. Role of cysteine proteases in malaria transmission
    KIM WILLIAMSON; Fiscal Year: 2006
    ..In addition to increasing our understanding of the genes involved, this information can be used to direct the design of cysteine protease inhibitors that will effectively block malaria transmission. ..
  57. Overexpression of membrane proteins in Tetrahymena
    THEODORE CLARK; Fiscal Year: 2006
    ..The technology described here is intended to better understand how these proteins function, leading ultimately to strategies for the treatment of serious disorders ranging from cancer to stoke. [unreadable] [unreadable]..
  58. Development of Preventative and Therapeutic HCV Vaccines
    David Milich; Fiscal Year: 2006
    ..It is anticipated that a NS3/4A DNA vaccine may be used for prophylactic or therapeutic applications either alone or in combination with E2-WHcAg hybrid particles. ..
  59. Antigenic Variation and Drug Resistance In P. falciparum
    Qin Cheng; Fiscal Year: 2006
    ..The project has the potential to benefit the health of a substantial proportion of the world's population. ..
  60. Immunoglobulin Allotypes in Hepatitis C Virus Infection
    JANARDAN PANDEY; Fiscal Year: 2008
    ..Results of this investigation will advance our understanding of the role of host genetic factors in clearance and persistence of hepatitis C virus infection. ..
  61. Immune response to Pneumocystis in simian model of AIDS
    Karen Norris; Fiscal Year: 2009
    ....
  62. Immunologic interaction of schistomiasis & acute malaria
    Kirsten Lyke; Fiscal Year: 2005
    ..abstract_text> ..
  63. Rapid antigen detection test for Dengue infection
    Raymond Houghton; Fiscal Year: 2008
    ..This virus is endemic in many tropical and sub-tropical regions of the world and can be fatal. It is also listed as a bioterrorism agent. Early diagnosis could lead to improved treatment outcomes. [unreadable] [unreadable] [unreadable]..
  64. T. cruzi immune evasion factors and vaccine design
    Karen Norris; Fiscal Year: 2008
    ..No vaccine is currently available to prevent or treat Chagas' disease and the proposed studies will test two vaccine candidates in experimental models. [unreadable] [unreadable] [unreadable]..
  65. Pneumocystis and COPD in a Simian Model of AIDS
    Karen Norris; Fiscal Year: 2009
    ..abstract_text> ..
  66. COMPLEMENT REGULATION IN TRYPANOSOMA CRUZI
    Karen Norris; Fiscal Year: 2005
    ..The successful completion of these aims will provide the first functional and immunological evaluation of a critical virulence factor of T. cruzi and provide information and reagents for the use of CRP as a vaccine candidate. ..
  67. PLASMODIUM VIVAX MSP-3 AND MSP-9 AS VACCINE IMMUNOGENES
    Mary Galinski; Fiscal Year: 2007
    ..vivax vaccine candidates based on these antigens and also serve to establish reliable procedures to test and evaluate antigens in non-human primate models for safety, immunogenicity and efficacy. ..
  68. Development of rapid detection tests for B pseudomallei
    Raymond Houghton; Fiscal Year: 2006
    ..These tools will be invaluable in detecting and monitoring acute and chronic B. pseudomallei infections both in endemic areas as well as following a bioterrorist attack. ..
  69. PNEUMOCYSTIS CARINII PNEUMONIA MODEL IN RHESUS MACAQUES
    Karen Norris; Fiscal Year: 2002
    ..carinii and determine the relevance of the nonhuman primate model, thus providing the means to study many aspects of PCP previously unapproachable due to the limitations of other animal models. ..