nuclear antigens

Summary

Summary: Immunologically detectable substances found in the CELL NUCLEUS.

Top Publications

  1. pmc Analysis of ku80-mutant mice and cells with deficient levels of p53
    D S Lim
    Lexicon Genetics, The Woodlands, Texas 77381 4287, USA
    Mol Cell Biol 20:3772-80. 2000
  2. pmc PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways
    Minli Wang
    University of Duisburg Essen, Medical School, Institute of Medical Radiation Biology, Hufeland Street 55, 45122 Essen, Germany
    Nucleic Acids Res 34:6170-82. 2006
  3. ncbi Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair
    J R Walker
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 412:607-14. 2001
  4. pmc Genetic steps of mammalian homologous repair with distinct mutagenic consequences
    Jeremy M Stark
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    Mol Cell Biol 24:9305-16. 2004
  5. ncbi The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen
    T M Gottlieb
    Wellcome CRC Institute, Cambridge, England
    Cell 72:131-42. 1993
  6. pmc Positional stability of single double-strand breaks in mammalian cells
    Evi Soutoglou
    National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Nat Cell Biol 9:675-82. 2007
  7. pmc Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells
    A J Pierce
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, and Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    Genes Dev 15:3237-42. 2001
  8. pmc The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay
    H Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    EMBO J 20:4987-97. 2001
  9. pmc Autophosphorylation of DNA-PKCS regulates its dynamics at DNA double-strand breaks
    Naoya Uematsu
    Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 177:219-29. 2007
  10. ncbi The DNA-dependent protein kinase
    G C Smith
    Wellcome Cancer Research Campaign Institute, Cambridge, CB2 1QR, UK
    Genes Dev 13:916-34. 1999

Research Grants

Detail Information

Publications266 found, 100 shown here

  1. pmc Analysis of ku80-mutant mice and cells with deficient levels of p53
    D S Lim
    Lexicon Genetics, The Woodlands, Texas 77381 4287, USA
    Mol Cell Biol 20:3772-80. 2000
    ..These data show that replicative senescence is caused by a p53-dependent cell cycle response to damaged DNA in ku80(-/-) cells and that p53 is essential for preventing very early onset of pro-B-cell lymphoma in ku80(-/-) mice...
  2. pmc PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways
    Minli Wang
    University of Duisburg Essen, Medical School, Institute of Medical Radiation Biology, Hufeland Street 55, 45122 Essen, Germany
    Nucleic Acids Res 34:6170-82. 2006
    ..This form of DSB repair is sensitive to PARP-1 inhibitors. The results define the function of PARP-1 in DSB repair and characterize a candidate pathway responsible for joining errors causing genomic instability and cancer...
  3. ncbi Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair
    J R Walker
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 412:607-14. 2001
    ..These features seem well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation...
  4. pmc Genetic steps of mammalian homologous repair with distinct mutagenic consequences
    Jeremy M Stark
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    Mol Cell Biol 24:9305-16. 2004
    ..These results imply that the proper genetic interplay of repair factors is essential to limit the mutagenic potential of DSB repair...
  5. ncbi The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen
    T M Gottlieb
    Wellcome CRC Institute, Cambridge, England
    Cell 72:131-42. 1993
    ..The implications of the unusual DNA binding mode and multicomponent nature of the DNA-PK are discussed...
  6. pmc Positional stability of single double-strand breaks in mammalian cells
    Evi Soutoglou
    National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Nat Cell Biol 9:675-82. 2007
    ..These results support a contact-first model in which chromosome translocations predominantly form among spatially proximal DSBs...
  7. pmc Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells
    A J Pierce
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, and Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    Genes Dev 15:3237-42. 2001
    ..Neither sister-chromatid exchange nor gene-targeting frequencies show a dependence on these NHEJ proteins. A Ku-modulated two-ended versus one-ended chromosome break model is presented to explain these results...
  8. pmc The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay
    H Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    EMBO J 20:4987-97. 2001
    ..Finally, we show that the composition of the EJC is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm...
  9. pmc Autophosphorylation of DNA-PKCS regulates its dynamics at DNA double-strand breaks
    Naoya Uematsu
    Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 177:219-29. 2007
    ..We suggest a model in which DNA-PK(CS) phosphorylation/autophosphorylation facilitates NHEJ by destabilizing the interaction of DNA-PK(CS) with the DNA ends...
  10. ncbi The DNA-dependent protein kinase
    G C Smith
    Wellcome Cancer Research Campaign Institute, Cambridge, CB2 1QR, UK
    Genes Dev 13:916-34. 1999
  11. pmc The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions
    H Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    EMBO J 19:6860-9. 2000
    ..Possible functions for this complex in nucleocytoplasmic transport of spliced mRNA, as well as the nonsense-mediated mRNA decay pathway, are discussed...
  12. pmc Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends
    Anne E Stellwagen
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 17:2384-95. 2003
    ..Thus, the interaction between Ku and TLC1 RNA enables telomerase to act at both broken and normal chromosome ends...
  13. ncbi Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis
    Haim Y Cohen
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115 USA
    Mol Cell 13:627-38. 2004
    ..We demonstrate that increased acetylation of Ku70 disrupts the Ku70-Bax interaction and coincides with cytoplasmic accumulation of CBP. These results shed light on the role of acetyltransferases as tumor suppressors...
  14. pmc Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing
    S J Boulton
    Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 17:1819-28. 1998
    ..These data provide important insights into DNA DSB repair and the linkage of this process to telomere length homeostasis and transcriptional silencing...
  15. ncbi A means to a DNA end: the many roles of Ku
    Jessica A Downs
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Nat Rev Mol Cell Biol 5:367-78. 2004
  16. pmc Deletion of Ku70, Ku80, or both causes early aging without substantially increased cancer
    Han Li
    Department of Molecular Medicine and Institute of Biotechnology, The University of Texas Health Science Center, San Antonio, Texas 78245, USA
    Mol Cell Biol 27:8205-14. 2007
    ..Thus, our observations suggest that the Ku heterodimer is important for longevity assurance in mice since divergent genetic backgrounds and/or environments likely account for these previously reported differences...
  17. pmc Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair
    Qi Ding
    College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824, USA
    Mol Cell Biol 23:5836-48. 2003
    ..However, autophosphorylation at these sites may not be sufficient for kinase disassembly...
  18. pmc Ku recruits the XRCC4-ligase IV complex to DNA ends
    S A Nick McElhinny
    Department of Biochemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
    Mol Cell Biol 20:2996-3003. 2000
    ..We have therefore defined cell-free conditions that reflect the genetic requirement for ligase IV in cellular end joining and consequently can explain in molecular terms why this factor is required...
  19. ncbi Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast
    S G Martin
    Swiss Institute for Experimental Cancer Research, Epalinges
    Cell 97:621-33. 1999
    ..Consistently, yKu- or SIR-deficient strains are hypersensitive to DNA-damaging agents. The release of yKu from telomeric chromatin may allow efficient scanning of the genome for DNA strand breaks...
  20. pmc Deletion of Ku86 causes early onset of senescence in mice
    H Vogel
    Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 96:10770-5. 1999
    ..These data indicate that Ku86-dependent chromosomal metabolism is important for determining the onset of age-specific changes characteristic of senescence in mice...
  21. pmc Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells
    M Takata
    Department of Molecular Immunology and Allergology, Kyoto University Medical School, Konoe Yoshida, Sakyo ku, Kyoto 606 8315, Japan
    EMBO J 17:5497-508. 1998
    ..These observations provide the first genetic evidence that both repair pathways play a role in maintaining chromosomal DNA during the cell cycle...
  22. ncbi Requirement for Ku80 in growth and immunoglobulin V(D)J recombination
    A Nussenzweig
    Department of Medical Physics, Memorial Sloan Kettering Cancer Centre, New York 10021, USA
    Nature 382:551-5. 1996
    ..The unexpected growth phenotype suggests a new and important link between Ku80 and growth control...
  23. ncbi Measurement of prompt DNA double-strand breaks in mammalian cells without including heat-labile sites: results for cells deficient in nonhomologous end joining
    Bo Stenerlow
    Division of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S 751 85 Uppsala, Sweden
    Radiat Res 159:502-10. 2003
    ..A study of cells deficient in nonhomologous end joining reveals that the residual fast repair response typically seen in such cells is solely due to repair at heat-labile sites and is not due to repair of prompt DSBs...
  24. pmc Ku70 acetylation mediates neuroblastoma cell death induced by histone deacetylase inhibitors
    Chitra Subramanian
    Department of Pediatrics, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 102:4842-7. 2005
    ..Whereas the Bax-binding ability of Ku70 allows it to block apoptosis in response to certain agents, it is also a molecular target for the action of HDACIs, and in this context, a mediator of NB cell death...
  25. ncbi Growth retardation and leaky SCID phenotype of Ku70-deficient mice
    Y Gu
    The Howard Hughes Medical Institute, Children s Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Immunity 7:653-65. 1997
    ..These unanticipated features of the Ku70-deficient phenotype with respect to lymphocyte development and V(D)J recombination may reflect differential functions of the three DNA-PK components...
  26. pmc Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system
    L Aravind
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
    Genome Res 11:1365-74. 2001
    ..A hypothesis is proposed that the Ku homolog, together with the associated gene products, comprise a previously unrecognized prokaryotic system for repair of double-strand breaks in DNA...
  27. pmc Development of autoimmune hepatitis-like disease and production of autoantibodies to nuclear antigens in mice lacking B and T lymphocyte attenuator
    Yoshihiro Oya
    Graduate School of Medicine, Chiba University, Chiba, Japan
    Arthritis Rheum 58:2498-510. 2008
    ..The purpose of this study was to investigate the role of BTLA in the regulation of immune homeostasis and the pathogenesis of autoimmunity...
  28. ncbi A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling
    Joanna Wysocka
    Laboratory of Chromatin Biology
    Nature 442:86-90. 2006
    ..We also identify a previously unknown function for the PHD finger as a highly specialized methyl-lysine-binding domain...
  29. ncbi Ku represses the HIV-1 transcription: identification of a putative Ku binding site homologous to the mouse mammary tumor virus NRE1 sequence in the HIV-1 long terminal repeat
    Laurence Jeanson
    CNRS UMR8532, Institut Gustave Roussy, PR2, 39 rue Camille Desmoulins, 94805 Villejuif, France
    J Biol Chem 277:4918-24. 2002
    ..Finally, a putative Ku-specific binding site was identified within the negative regulatory region of the HIV-1 long terminal repeat, which may account for this repression of transcription...
  30. pmc NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway
    M Frank-Vaillant
    Laboratoire du Cycle Cellulaire, Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Genes Dev 15:3005-12. 2001
    ..Increasing the dosage of the Lif2p protein can suppress the NHEJ defect in a/alpha cells. Together, these results indicate that NHEJ regulation by mating type is achieved, at least in part, by a regulation of Lif2p activity...
  31. ncbi Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage
    Jacob Falck
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 434:605-11. 2005
    ....
  32. ncbi The SAND domain structure defines a novel DNA-binding fold in transcriptional regulation
    M J Bottomley
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Struct Biol 8:626-33. 2001
    ..The evolutionarily conserved SAND domain defines a new DNA binding fold that is involved in chromatin-associated transcriptional regulation...
  33. ncbi Evidence for the existence of an HP1-mediated subcode within the histone code
    Gwen Lomberk
    Gastroenterology Research Unit, Department of Medicine, and Mayo Clinic Cancer Center, Rochester, MN 55605, USA
    Nat Cell Biol 8:407-15. 2006
    ....
  34. ncbi Genetic determination of T cell help in loss of tolerance to nuclear antigens
    Yifang Chen
    Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA
    J Immunol 174:7692-702. 2005
    ....
  35. pmc Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase
    Nicholas S Y Ting
    Department of Biochemistry and Molecular Biology, Cancer Biology Research Group, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Nucleic Acids Res 33:2090-8. 2005
    ....
  36. pmc Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination In vivo
    H Ouyang
    Department of Medical Physics and Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    J Exp Med 186:921-9. 1997
    ..These results suggest that distinct but overlapping repair pathways may mediate DNA DSB repair and V(D)J recombination...
  37. ncbi DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks
    Jerome Drouet
    Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077 Toulouse, Cedex 4, France
    J Biol Chem 280:7060-9. 2005
    ..Altogether these results offer insights into the interplay between key NHEJ proteins during this repair process in the cell...
  38. ncbi Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination
    G E Taccioli
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA
    Science 265:1442-5. 1994
    ..Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase complex in those same processes...
  39. ncbi Up-regulation of DNA-dependent protein kinase activity and Sp1 in colorectal cancer
    Yoshio Hosoi
    Department of Radiation Research, Faculty of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Int J Oncol 25:461-8. 2004
    ..These results suggest that DNA-PK activity and protein- and mRNA-levels of Ku70, Ku80 and DNA-PKcs were elevated in tumor tissues in patients with colorectal cancer because of elevated Sp1 protein levels in tumor tissues...
  40. ncbi Regulation of CHK2 by DNA-dependent protein kinase
    Jia Li
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 280:12041-50. 2005
    ..Ionizing radiation-induced Chk2 phosphorylation was wortmannin-sensitive in ATM-defective cells with depleted ATR. These results suggest that DNA-PK augments ATM and ATR in activation of Chk2 by DNA damage...
  41. ncbi Impact of the KU80 pathway on NHEJ-induced genome rearrangements in mammalian cells
    Josee Guirouilh-Barbat
    UMR CNRS CEA 217, Laboratoire d étude des Mécanismes de la Recombinaison, Direction des Sciences du Vivant, Departement de Radiobiologie et Radiopathologie, 18 route du Panorama, 92265 Fontenay aux Roses Cedex, France
    Mol Cell 14:611-23. 2004
    ..Thus these processes should represent prominent pathways for DSB-induced genetic instability in mammalian cells...
  42. pmc Ku complex interacts with and stimulates the Werner protein
    M P Cooper
    Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Genes Dev 14:907-12. 2000
    ..Although Ku proteins had no effect on ATPase or helicase activity, they strongly stimulated specific exonuclease activity. These results suggest that WRNp and the Ku complex participate in a common DNA metabolic pathway...
  43. ncbi The Ku-dependent non-homologous end-joining but not other repair pathway is inhibited by high linear energy transfer ionizing radiation
    Hongyan Wang
    Department of Radiation Oncology and Kimmel Cancer Center of Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
    DNA Repair (Amst) 7:725-33. 2008
    ....
  44. ncbi Correlation between MIB-1 and other proliferation markers: clinical implications of the MIB-1 cutoff value
    Frederique Spyratos
    Laboratoire d Oncobiologie, Centre Rene Huguenin, Saint Cloud, France
    Cancer 94:2151-9. 2002
    ..The objectives of this study were to compare MIB-1 evaluation to other methods of measuring cell proliferation, with a view to refining the cutoff used to classify tumors with low and high proliferation rates in therapeutic trials...
  45. pmc Clinical value of multiplexed bead-based immunoassays for detection of autoantibodies to nuclear antigens
    Erik Avaniss-Aghajani
    Primex Clinical Laboratories, 16742 Stagg Street 120, Van Nuys, CA 91406, USA
    Clin Vaccine Immunol 14:505-9. 2007
    ....
  46. ncbi Dimerization, translocation and localization of Ku70 and Ku80 proteins
    Manabu Koike
    Radiation Hazards Research Group, National Institute of Radiological Sciences, 4 9 1 Anagawa, Inage Ku, Chiba 263 8555, Japan
    J Radiat Res 43:223-36. 2002
    ..The mechanism that regulates for nuclear localization of Ku70 and Ku80 appears to play, at least in part, a key role in regulating the physiological function of Ku in vivo...
  47. ncbi Multiple interactions between SRm160 and SR family proteins in enhancer-dependent splicing and development of C. elegans
    D Longman
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom
    Curr Biol 11:1923-33. 2001
    ..The SRm160 splicing coactivator, an SR-related nuclear matrix protein of 160 kDa, is important for the splicing of specific constitutive and ESE-dependent pre-mRNAs...
  48. ncbi Studies on the mode of Ku interaction with DNA
    Daniele Arosio
    International Centre for Genetic Engineering and Biotechnology, Padriciano, 99, Trieste I 34012, Italy
    J Biol Chem 277:9741-8. 2002
    ..Thus, we demonstrate that there is no cooperative interaction among the Ku heterodimers binding longer substrates...
  49. pmc Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences
    Jia Lin Ma
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:8820-8. 2003
    ..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
  50. ncbi Lymphocytopenia in lupus erythematosus: close in vivo association to autoantibodies targeting nuclear antigens
    J Wenzel
    Department of Dermatology, University of Bonn, Bonn, Germany
    Br J Dermatol 150:994-8. 2004
    ..Antilymphocyte antibodies are regarded as the most likely rationale for the decreased cell numbers; however, their relevance has not been confirmed so far...
  51. pmc SRm160 splicing coactivator promotes transcript 3'-end cleavage
    Susan McCracken
    Banting and Best Department of Medical Research, C H Best Institute, University of Toronto, Toronto, Ontario, Canada
    Mol Cell Biol 22:148-60. 2002
    ..The results provide evidence for a role for SRm160 in mRNA 3'-end formation and suggest that the level of this splicing coactivator is important for the proper coordination of pre-mRNA processing events...
  52. ncbi Ku70: a candidate tumor suppressor gene for murine T cell lymphoma
    G C Li
    Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell 2:1-8. 1998
    ..These findings directly demonstrate that Ku70 deficiency facilitates neoplastic growth and suggest a novel role of the Ku70 locus in tumor suppression...
  53. ncbi Identification and characterization of a leukocyte-specific component of the nuclear body
    D B Bloch
    Department of Medicine, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    J Biol Chem 271:29198-204. 1996
    ..The location of Sp140 in the NB, and expression of this gene in cells involved in host defense, suggest that Sp140 may be involved in the pathogenesis of acute promyelocytic leukemia and viral infection...
  54. ncbi Effects of DNA nonhomologous end-joining factors on telomere length and chromosomal stability in mammalian cells
    F d'Adda di Fagagna
    Wellcome CRC Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
    Curr Biol 11:1192-6. 2001
    ..We also observe higher genomic instability in Ku-deficient cells than in XRCC4-null cells. This suggests that chromosomal instability of Ku-deficient cells results from a combination of compromised telomere stability and defective NHEJ...
  55. pmc Hypersensitivity of Ku80-deficient cell lines and mice to DNA damage: the effects of ionizing radiation on growth, survival, and development
    A Nussenzweig
    Departments of Medical Physics and Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 94:13588-93. 1997
    ..The response of Ku80-deficient cell lines and mice to DNA-damaging agents provides important insights into the role of Ku80 in growth regulation, lymphocyte development, and DNA repair...
  56. pmc Cellular localization, expression, and structure of the nuclear dot protein 52
    T Sternsdorf
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, D 20251 Hamburg, FRG
    J Cell Biol 138:435-48. 1997
    ..Finally, unlike Sp100 and PML, NDP52 expression is neither markedly enhanced nor localization detectably altered by type I and II IFNs...
  57. ncbi Interaction of human Ku70 with TRF2
    K Song
    Department of Biochemistry, University of Ulsan College of Medicine, Seoul, South Korea
    FEBS Lett 481:81-5. 2000
    ..This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70...
  58. pmc Structural model of full-length human Ku70-Ku80 heterodimer and its recognition of DNA and DNA-PKcs
    Angel Rivera-Calzada
    Centro de Investigaciones Biologicas CIB, Spanish National Research Council CSIC, Ramiro de Maeztu 9, Campus Complutense University, Madrid 28040, Spain
    EMBO Rep 8:56-62. 2007
    ....
  59. pmc A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses
    Shin ichiro Kanno
    Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Aobaku, Sendai, Japan
    EMBO J 26:2094-103. 2007
    ..Various treatments that produce double-strand breaks induce formation of PALF foci, which fully coincide with gammaH2AX foci. Thus, PALF and the CYR motif may play important roles in DNA repair of higher eukaryotes...
  60. ncbi Ku autoantigen: a multifunctional DNA-binding protein
    R Tuteja
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi
    Crit Rev Biochem Mol Biol 35:1-33. 2000
    ..The mechanism underlying the regulation of all the diverse functions of Ku is still obscure...
  61. ncbi Dimerization and nuclear localization of ku proteins
    M Koike
    Genome Research Group, National Institute of Radiological Sciences, 4 9 1 Anagawa, Inage Ku, Chiba 263 8555, Japan
    J Biol Chem 276:11167-73. 2001
    ..Our results indicate that each Ku subunit can translocate to the nucleus not only through its own NLS but also through heterodimerization with each other...
  62. pmc Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae
    G T Milne
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:4189-98. 1996
    ..Therefore, Ku and Rad50 participate in an end-joining pathway that is distinct from homologous recombinational repair. Yeast DNA end joining is functionally analogous to DSB repair and V(D)J recombination in mammalian cells...
  63. ncbi V(D)J recombination in Ku86-deficient mice: distinct effects on coding, signal, and hybrid joint formation
    M A Bogue
    Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA
    Immunity 7:37-47. 1997
    ..Furthermore, Ku86(-/-) joints are unexpectedly devoid of N-region diversity, suggesting a novel role for Ku in the addition of N nucleotides by terminal deoxynucleotidyl transferase...
  64. ncbi Synergistic role of Ku80 and poly(ADP-ribose) polymerase in suppressing chromosomal aberrations and liver cancer formation
    Wei Min Tong
    International Agency for Research on Cancer IARC, 69008 Lyon, France
    Cancer Res 62:6990-6. 2002
    ....
  65. pmc The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing
    T Durfee
    Center for Molecular Medicine Institute of Biotechnology, University of Texas Health Science Center at San Antonio 78245
    J Cell Biol 127:609-22. 1994
    ..Thus, the amino terminus of p110RB may function in part to facilitate the binding of growth promoting factors at subnuclear regions actively involved in RNA metabolism...
  66. pmc Formation of NHEJ-derived reciprocal chromosomal translocations does not require Ku70
    David M Weinstock
    Department of Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Nat Cell Biol 9:978-81. 2007
    ..Surprisingly, translocations are increased in cells deficient for the nonhomologous end-joining (NHEJ) protein Ku70, implicating non-canonical joining pathways in their etiology...
  67. pmc The SRm160/300 splicing coactivator subunits
    B J Blencowe
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    RNA 6:111-20. 2000
    ..The results indicate that SRm160 may be the more critical component of the SRm160/300 coactivator in the splicing of SRm160/300-dependent pre-mRNAs...
  68. pmc Productive and nonproductive complexes of Ku and DNA-dependent protein kinase at DNA termini
    R B West
    Departments of Pathology and of Biochemistry and Molecular Biology, University of Southern California School of Medicine, Los Angeles, California 90033, USA
    Mol Cell Biol 18:5908-20. 1998
    ..Both the productive and nonproductive configurations are likely to be of physiologic importance, depending on the distance of the dsDNA break site to other protein complexes, such as nucleosomes...
  69. ncbi A biochemically defined system for mammalian nonhomologous DNA end joining
    Yunmei Ma
    University of Southern California Norris Comprehensive Cancer Center, Room 5428, Department of Pathology, University of Southern California Keck School, of Medicine, 1441 Eastlake Avenue, MC9176, Los Angeles, California 90033, USA
    Mol Cell 16:701-13. 2004
    ..The XRCC4:DNA ligase IV complex is able to ligate one strand that has only minimal base pairing with the antiparallel strand. This important aspect of the ligation leads to an iterative strand-processing model for the steps of NHEJ...
  70. ncbi Protection of mammalian telomeres
    Titia de Lange
    Laboratory for Cell Biology and Genetics, The Rockefeller University, New York, NY 10021, USA
    Oncogene 21:532-40. 2002
    ....
  71. pmc Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair
    Nicole Bennardo
    Department of Radiation Biology, Beckman Research Institute of City of Hope, Duarte, California, United States of America
    PLoS Genet 4:e1000110. 2008
    ..However, at later steps of repair, alt-NHEJ is a mechanistically distinct pathway of DSB repair, and thus may play a unique role in mutagenesis during cancer development and therapy...
  72. ncbi Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF
    Haitao Li
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 442:91-5. 2006
    ..Our findings call attention to the PHD finger as a previously uncharacterized chromatin-binding module found in a large number of chromatin-associated proteins...
  73. ncbi Human Ku70/80 associates physically with telomerase through interaction with hTERT
    Weihang Chai
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9039, USA
    J Biol Chem 277:47242-7. 2002
    ..The results presented here are the first to report that Ku associates with hTERT, and this interaction may function to regulate the access of telomerase to telomeric DNA ends...
  74. pmc In vivo association of Ku with mammalian origins of DNA replication
    O Novac
    McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Mol Biol Cell 12:3386-401. 2001
    ..The results indicate a novel function for Ku as an origin binding-protein, which acts at the initiation step of DNA replication and dissociates after origin firing...
  75. ncbi Breast cancer risk associated with genotypic polymorphism of the nonhomologous end-joining genes: a multigenic study on cancer susceptibility
    Yi Ping Fu
    Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan
    Cancer Res 63:2440-6. 2003
    ....
  76. pmc Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair
    Kiran N Mahajan
    Lineberger Comprehensive Cancer Center, Mason Farm Road, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 22:5194-202. 2002
    ..Our data thus support an important role for pol mu in the end-joining pathway for repair of double-strand breaks...
  77. ncbi The biology of Ku and its potential oncogenic role in cancer
    Charles Gullo
    Multiple Myeloma Research Laboratory, MMRL, Singapore Health Services, SingHealth, 7 Hospital Drive, Block A 02 05, Singapore 169611, Republic of Singapore
    Biochim Biophys Acta 1765:223-34. 2006
    ..These studies may also increase our understanding of how Ku autoantibodies are generated in autoimmune diseases...
  78. ncbi DNA repair, genome stability, and aging
    David B Lombard
    Howard Hughes Medical Institute, The Children s Hospital, Department of Genetics, Harvard Medical School and, The CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Cell 120:497-512. 2005
    ..We review Sir2, a factor linking genomic stability, metabolism, and aging. We conclude with a general discussion of the role of mutant mice in aging research and avenues for future investigation...
  79. ncbi Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment
    Patrick Calsou
    Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Cedex 4, Toulouse, France
    J Mol Biol 326:93-103. 2003
    ..These findings extend the current knowledge of the assembly of NHEJ repair proteins on DNA termini and substantiate the hypothesis of a scaffolding role of DNA-PK towards other components of the NHEJ DNA repair process...
  80. ncbi A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis
    Y Gao
    Howard Hughes Medical Institute, The Children s Hospital, Department of Genetics, Harvard University Medical School, Boston, Massachusetts 02115, USA
    Cell 95:891-902. 1998
    ..Our findings demonstrate that differentiating lymphocytes and neurons strictly require the XRCC4 and DNA ligase IV end-joining proteins and point to the general stage of neuronal development in which these proteins are necessary...
  81. ncbi DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer
    D W Chan
    Department of Biological Sciences, University of Calgary, Canada
    Biochemistry 38:1819-28. 1999
    ..Interestingly, none of the phosphorylation sites identified in Ku correspond to the serine-glutamine consensus for DNA-PK phosphorylation, consistent with previous reports that DNA-PK can recognize additional phosphorylation motifs...
  82. ncbi NBS1 regulates a novel apoptotic pathway through Bax activation
    Kenta Iijima
    Department of Environmental Sciences, Faculty of Science, Ibaraki University, Bunkyo 2 1 1, Mito, Ibaraki, Japan
    DNA Repair (Amst) 7:1705-16. 2008
    ..Thus, NBS1 is a key protein involved in the prevention of carcinogenesis, not only through the precise repair of damaged DNA by homologous recombination (HR) but also by its role in the elimination of inappropriately repaired cells...
  83. ncbi Interplay of p53 and DNA-repair protein XRCC4 in tumorigenesis, genomic stability and development
    Y Gao
    Howard Hughes Medical Institute, The Children s Hospital, and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 404:897-900. 2000
    ..Our findings support a crucial role for the non-homologous end-joining pathway as a caretaker of the mammalian genome, a role required both for normal development and for suppression of tumours...
  84. pmc Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites
    Xianming Mo
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    Mol Cell Biol 22:8088-99. 2002
    ..Tethering of double-strand break repair proteins to defined subnuclear structures may also be advantageous in maintenance of genome stability...
  85. ncbi Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends
    Steven A Roberts
    Lineberger Comprehensive Cancer Center and Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:10605-13. 2007
    ..We suggest a model where Ku utilizes an unusual characteristic of its three-dimensional structure to recognize certain protein-occluded ends without the extensive remodeling of chromatin structure required by other DNA repair pathways...
  86. ncbi A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly
    A Merdes
    Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla 92093 0660, USA
    Cell 87:447-58. 1996
    ..Our findings suggest that NuMA forms bifunctional complexes with cytoplasmic dynein and dynactin that can tether microtubules at the spindle poles and that are essential for mitotic spindle pole assembly and stabilization...
  87. ncbi Narrow-band UVB-induced externalization of selected nuclear antigens in keratinocytes: implications for lupus erythematosus pathogenesis
    Adam Reich
    Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, Poland
    Photochem Photobiol 85:1-7. 2009
    ..We applied atomic force microscopy (AFM) to visualize cell surface structures expressing nuclear antigens upon apoptosis following NB-UVB irradiation...
  88. pmc Ku recruits XLF to DNA double-strand breaks
    Ken ichi Yano
    Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, 5801 Forest Park Road, Dallas, TX 75390 9187, USA
    EMBO Rep 9:91-6. 2008
    ..Our observations showed the direct involvement of XLF in the dynamic assembly of the NHEJ machinery and provide mechanistic insights into DSB recognition...
  89. pmc Alternative endings
    James E Haber
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 105:405-6. 2008
  90. ncbi The interferon (IFN)-stimulated gene Sp100 promoter contains an IFN-gamma activation site and an imperfect IFN-stimulated response element which mediate type I IFN inducibility
    T Grötzinger
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, 20251 Hamburg, Germany
    J Biol Chem 271:25253-60. 1996
    ..These data demonstrate a novel arrangement of a nonoverlapping IFN-gamma activation site and an IFN-stimulated response element mediating type I IFN inducibility, previously not reported for other IFN-stimulable promoters...
  91. ncbi Distinct faces of the Ku heterodimer mediate DNA repair and telomeric functions
    Albert Ribes-Zamora
    Department of Pediatrics, Hematology Oncology Section, Baylor College of Medicine, One Baylor Plaza, BCM225, Houston, Texas 77030 3411, USA
    Nat Struct Mol Biol 14:301-7. 2007
    ..We propose a 'two-face' model for Ku and that divergent evolution of these faces allowed Ku's dual role in NHEJ and telomere maintenance...
  92. pmc The Saccharomyces cerevisiae Ku autoantigen homologue affects radiosensitivity only in the absence of homologous recombination
    W Siede
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Genetics 142:91-102. 1996
    ....
  93. ncbi Ku protein targeting by Ku70 small interfering RNA enhances human cancer cell response to topoisomerase II inhibitor and gamma radiation
    Iraimoudi S Ayene
    Department of Radiation Oncology, School of Medicine, University of Pennsylvania, 195 John Morgan Building, Philadelphia, PA 19104 6072, USA
    Mol Cancer Ther 4:529-36. 2005
    ..Studies with HCT116 cells using the same Ku70 siRNA (CS #5) showed a direct correlation between expression of Ku70 and sensitization to radiation and etoposide treatments...
  94. pmc Practical evaluation of methods for detection and specificity of autoantibodies to extractable nuclear antigens
    Susan M Orton
    McLendon Clinical Laboratories, University of North Carolina Healthcare, Chapel Hill, 27514, USA
    Clin Diagn Lab Immunol 11:297-301. 2004
    Detection and specificity of autoantibodies against extractable nuclear antigens (ENA) play a critical role in the diagnosis and management of autoimmune disease...
  95. pmc The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques
    A Gregory Bruce
    Center for Childhood Infection and Prematurity Research, Seattle Children s Research Institute, Seattle, WA 98101 1304, USA
    Virol J 6:205. 2009
    ..KSHV ORF59 is antigenic in the infected host and is used as a marker for virus activation and replication...
  96. ncbi Evaluation of a line immunoblot assay for detection of antibodies recognizing extractable nuclear antigens
    H E Prince
    MRL Reference Laboratory, Cypress, California 90630, USA
    J Clin Lab Anal 12:320-4. 1998
    Sera (n = 90) giving positive results in a screening test for antibodies to extractable nuclear antigens (ENAs) were tested in a line immunoblot assay that measures antibody reactivity with individual ENAs in a single test field...
  97. ncbi Efficiency of different strategies to detect autoantibodies to extractable nuclear antigens
    Delia Almeida González
    Hospital Universitario Ntra Sra de la Candelaria, Tenerife, Canary Islands, Spain
    J Immunol Methods 360:89-95. 2010
    Autoantibodies to extractable nuclear antigens (anti-ENA) are identified mainly in samples positive for antinuclear antibodies (ANA)...
  98. ncbi Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element
    N Warriar
    Department of Molecular Endocrinology, Laval University Hospital Center, 2705 Boulevard Laurier, Sainte Foy, G1V 4G2, Quebec, Canada
    J Biol Chem 271:18662-71. 1996
    ..Partial peptide fingerprinting of GREF1 and GREF2 using alpha-chymotrypsin and immunoblotting with Ab 111 and Ab N3H10 confirmed their identities as Ku80 and Ku70, respectively...
  99. ncbi Back to the roots of a new exon--the molecular archaeology of a SP100 splice variant
    P Rogalla
    Center of Human Genetics and Genetic Counselling, University of Bremen, Bremen, Germany
    Genomics 63:117-22. 2000
    ..To our knowledge this is the first example of a retropseudogene becoming part of an active gene in which both parental parts are well characterized and remain in-frame with their cDNA...
  100. ncbi A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera
    F Jafri
    Program in Gene Regulation, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    J Immunol Methods 251:53-61. 2001
    ..The method will be advantageous for high-throughput screening for antibodies and other ligands that bind the assembled DNA-dependent protein kinase complex...
  101. pmc Autoantibodies to extractable nuclear antigens: making detection and interpretation more meaningful
    Tri Giang Phan
    Central Sydney Immunology Laboratory, Department of Clinical Immunology, Royal Prince Alfred Hospital, Camperdown, New South Wales 2050, Australia
    Clin Diagn Lab Immunol 9:1-7. 2002

Research Grants62

  1. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
    ..including systemic lupus (SLE), in which heavily mutated and class-switched (mainly IgG) autoantibodies to nuclear antigens, as secreted by large numbers of plasma cells, produce widespread tissue and organ injury...
  2. The Role of Sex in Self Antigen Generation in SLE
    David Stephen Pisetsky; Fiscal Year: 2010
    ..In SLE, the nuclear antigens inducing responses are most likely released from dead and dying cells, with the rates of cell death and dead ..
  3. MECHANISMS OF AUTOIMMUNITY IN SLE
    David Stephen Pisetsky; Fiscal Year: 2013
    ..As shown in in vivo and in vitro experiments, nuclear antigens, including DNA, RNA and the high mobility group protein 1 (HMGB1), have activity as alarmins...
  4. The Effects of C-Reactive Protein on Autoimmunity
    Terry W Du Clos; Fiscal Year: 2013
    ..Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by autoantibodies against nuclear antigens. The etiology of SLE is incompletely understood, but there is a strong genetic component...
  5. Developing small molecule therapeutics for lupus
    Thomas R Coleman; Fiscal Year: 2012
    ..SLE is characterized by the production of autoantibodies, many of which have specificity for nuclear antigens, in particular, DNA. These anti-DNA antibodies are now known to cross-react avidly with non-DNA (e.g...
  6. Roles of Epstein-Barr virus nuclear antigens 2 and LP in B cell proliferation
    Elliott D Kieff; Fiscal Year: 2013
    ....
  7. Progesterone &Estrogen Differentially Regulate DC Function in Lupus Autoimmunity
    Grant Hughes; Fiscal Year: 2012
    ..In SLE, however, immune complexes (ICs) comprised of autoantibodies (autoAbs) and nuclear antigens (Ags), through TLRs 7 and 9, activate myeloid DCs (mDCs) and stimulate plasmacytoid DCs (pDCs) to make large ..
  8. The Renal Pathogenicity of Anti-DNA Antibodies
    Chaim Putterman; Fiscal Year: 2013
    ..Alternatively, anti-DNA antibodies may be pathogenic not by virtue of binding to nuclear antigens, but rather via binding to cross reactive renal targets...
  9. Somatic Hypermutation in SLE
    Lawrence J Wysocki; Fiscal Year: 2013
    ..mutant ANA generated by AID promote the escape of germline ANA-producing B cells by obscuring or removing nuclear antigens that would otherwise render the B cells tolerant...
  10. T Cell Tolerance &Autoimmunity to Nuclear Antigen La
    A Darise Farris; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): RNA-binding nuclear antigens are a major class of autoantigen targeted in systemic autoimmune diseases, including systemic lupus erythematosus (SLE) and Sjvgren's syndrome (SS)...
  11. Microparticles as a Source of Nuclear Antigens in Systemic Lupus Erythematosus
    David Stephen Pisetsky; Fiscal Year: 2010
    ..The goal of these investigations is to elucidate the role of microparticles (MPs) as a key source of nuclear antigens in systemic lupus erythematosus (SLE) and characterize these structures as components of pathogenic immune ..
  12. Excessive Receptor Editing and Generation of Autoreactive Antibodies in SLE
    Zhixin Zhang; Fiscal Year: 2012
    ..lupus erythematosus (SLE) is characterized by the over production of high affinity autoantibodies against nuclear antigens. The disease associated autoantibodies play important roles in SLE pathogenesis, disease progression, and ..
  13. IMMUNOPATHOGENESIS OF LUPUS
    Charles S Via; Fiscal Year: 2012
    ..that cytotoxic T lymphocytes (CTL) are an important early mechanism that limits breaks in tolerance to nuclear antigens and prevents lupus development by eliminating activated autoreactive B cells...
  14. Epstein-Barr Virus Nuclear Protein in B Cell Growth Transformation
    Elliott D Kieff; Fiscal Year: 2013
    ..The Latency III EBV nuclear antigens EBNA1, 2, LP, 3A, 3C, and the membrane protein LMP1 are required for EBV B-cell transformation...
  15. Functional Relationships Between the Lupus Susceptibility Loci Lyn and Ets1
    Anne B Satterthwaite; Fiscal Year: 2013
    ..provided by applicant): Systemic lupus erythematosus (SLE) is characterized by loss of tolerance to nuclear antigens, autoantibody production, and immune complex mediated damage to multiple organs...
  16. Mechanisms of B-Cell Tolerance in a Mouse Model of Lupus
    Allison Sang; Fiscal Year: 2013
    ..of this potentially fatal disorder is the production of autoantibodies, by autoreactive B-cells, against nuclear antigens. Currently, there is no cure for lupus and multiple B-cell targeted therapies have not been successful in ..
  17. Lupus and the inhibitory dual receptor hypothesis
    GREGG JOSHUA SILVERMAN; Fiscal Year: 2013
    ..whether and how anti-ACM antibodies block the pro-inflammatory effects of pathogenic IgG autoantibodies to nuclear antigens, postulated to trigger TLR7 and TLR9...
  18. TRICHINELLOSIS AND REGULATION OF HOST GENE EXPRESSION
    Douglas Jasmer; Fiscal Year: 2001
    ..Methods were developed to extract these nuclear antigens (NA)...
  19. Tolerance to the Nuclear Antigen La
    A Darise Farris; Fiscal Year: 2006
    ..In order for tolerance and autoimmunity to nuclear antigens to be comprehended, it is therefore imperative that nuclear antigens be studied as a special class of antigen...
  20. FUNCTION OF QUIESCENT CELLS IN PEDIATRIC LEUKEMIA
    Michael Andreeff; Fiscal Year: 1992
    ..These "G0-" cells will be further analyzed by multiparameter flow cytometric investigations of the nuclear antigens K167 and PCNA ("Cyclin") of chromatin structure in-situ and of a novel proliferation-related cell surface ..
  21. Testing in Vivo Roles of TLRs in Systemic Autoimmunity
    Mark Shlomchik; Fiscal Year: 2004
    ..diseases focuses on a specific set of autoantigens, but it is not clear why certain molecules, such as nuclear antigens and self-IgG are targets in autoimmune diseases...
  22. Role of SF Gene Cluster in Autoimmunity
    Edward Wakeland; Fiscal Year: 2007
    ..a seven-gene cluster of CD2 family genes (SF gene cluster) as causative for a breach in immune tolerance to nuclear antigens. The SF cluster contains CD48, CD84, 2B4, SLAM, Ly9, Lyl08, and CSI...
  23. AUTOIMMUNITY IN MURINE GRAFT-VERSUS-HOST DISEASE
    BRIAN KOTZIN; Fiscal Year: 1993
    ..and disease in the GVH model is dependent on an interaction of specific B cells with their respective nuclear antigens, and the stimulation of these B cells by donor CD4+ T cells that are specific for allogeneic class II MHC ..
  24. NOVEL MITOGENIC REGULATORY GENE
    Irwin Gelman; Fiscal Year: 1999
    ..Two glycine/arginine/lysine-rich regions, homologous to Epstein-Barr virus nuclear antigens, are found in the N- terminal domain of the putative 322 product...
  25. EBV TRANSFORMATION OF B LYMPHOCYTES--EBNA REGULATION
    David Gutsch; Fiscal Year: 2001
    ..Wp directs the early expression of Epstein-Barr virus nuclear antigens (EBNA)...
  26. NATURAL HISTORY AND TRANSMISSION OF HHV8/KSHV
    Dennis Osmond; Fiscal Year: 2001
    ..Health Study, will be used to identify new seroconversions for antibodies to HHV-8/KSHV latency- associated nuclear antigens (LANA) and to obtain prospective samples of blood, semen, and saliva from men with pre-existing HHV-8/KSHV ..
  27. Screening of Epstein Barr Virus Replication (RMI)
    Elliott Kieff; Fiscal Year: 2004
    ..In B cells EBV can establish 3 types of latency. Latency Ill, EBV expresses 6 EB Nuclear antigens (EBNAs) and two integral membrane proteins (LMP1 and 2) and causes lymphocyte proliferation, which is ..
  28. Molecular Basis for Overcoming Tolerance to Sm
    BARBARA VILEN; Fiscal Year: 2007
    ..Disease occurs when B cell tolerance is overcome and cells specific for nuclear antigens renew signal transduction through the BCR leading to autoantibody secretion...
  29. ANIMAL MODEL FOR SCREENING HUMAN AUTOANTIBODIES
    KLAUS BRASCH; Fiscal Year: 1992
    ..have not been linked directly with disease pathogenesis, many are directed against universally important nuclear antigens. Consequently, assays based on a chicken tissue which can be fully reprogrammed by a single exposure to ..
  30. BIOLOGY OF PROLIFERATING CELL NUCLEAR ANTIGENS
    Renato Baserga; Fiscal Year: 2000
    ....
  31. Genetic dissection of Sle2 contribution to SLE pathogenesis
    Laurence Morel; Fiscal Year: 2010
    ..each S/e2 locus, and 3) determine which of the three S/e2 loci is responsible for the loss of tolerance to nuclear antigens using the 56R model. 3. To identify the genes corresponding to S/e2a. Sle2b, and S/e2c...
  32. LYMPHOMAS IN SCID MICE ENGRAFTED WITH HIV-1 LYMPHOCYTES
    Thomas McFarland; Fiscal Year: 1993
    ..in various stages of disease, who are either seropositive or seronegative for antibodies to the EBV nuclear antigens. Lymphomas that develop in these mice will be characterized for their clonality by the study of mouse sera ..
  33. Dendritic cell mediated modulation of tolerance by apoptotic cells in aging
    Anshu Agrawal; Fiscal Year: 2007
    ..Apoptosis is increased in aged humans and during apoptosis, nuclear antigens are expressed on cell membranes and may serve to induce autoreactivity if apoptotic cells are not swiftly and ..
  34. CELL SURFACE DNA - AN ANALYSIS OF SPECIFIC RECEPTORS
    Robert Bennett; Fiscal Year: 1992
    ..Autoimmunity to nuclear antigens presents a puzzling enigma, as they exist in a "protected" location and are relatively non-immunogenic; the ..
  35. TOLL RECEPTORS IN MACROPHAGE & DENDRITIC CELL ACTIVATION
    Anne Stevens; Fiscal Year: 2003
    ..erythematosus (SLE) patients, can activate macrophages in these patients, and contain within their blebs the nuclear antigens that are recognized by SLE antibodies, which may be factors in disease development...
  36. MHC Class II-restricted Cytoplasmic Antigen Presentation
    Janice Blum; Fiscal Year: 2007
    ..Remarkably, epitopes from endogenous cytoplasmic and nuclear antigens also gain access to class II molecules...
  37. Role of complement C4 in immune tolerance
    Michael Carroll; Fiscal Year: 2006
    ..several approaches will be tested to study the potential of C4 reconstitution to induce tolerance to nuclear antigens in adult mice with developed lupus: 1) bone marrow transfer to reconstitute myeloid-derived C4; 2) in vivo ..
  38. Impact of lupus susceptibility loci on B-cell tolerance
    Chandra Mohan; Fiscal Year: 2007
    ..2) To ascertain the impact of lupus susceptibility loci (Slela, Slelb, Sle2, Sle3 and Sle5) to non-nuclear antigens, using the anti-HEL immunoglobulin transgenic model...
  39. KU COMPLEX AND TELOMERE END PROTECTION
    ALISON BERTUCH; Fiscal Year: 2003
    ..Dr. Lundblad's laboratory in the Department of Molecular and Human Genetics at the Baylor College of Medicine provides an excellent environment in which to develop my scientific skills. ..
  40. DENVER AUTOIMMUNITY CENTER OF EXCELLENCE
    V Holers; Fiscal Year: 2007
    ..This latter project involves a newly established collaboration with a separate institution and group of clinicians skilled in the clinical investigation of RA. ..
  41. Investigation of xrcc5 Mutant Mice
    EDWARD HASTY; Fiscal Year: 2005
    ..The role of the tumor suppression protein, p53, will be analyzed for its impact on oncogenesis in ku80-mutant mice with specific attention given to mammary tissue in a defined mouse model. ..
  42. Evolution of Autoimmunity in Rheumatoid Arthritis
    V Holers; Fiscal Year: 2005
    ..abstract_text> ..
  43. MOUSE MEMBRANE RCA PROTEINS IN THE IMMUNE RESPONSE
    V Holers; Fiscal Year: 2008
    ..Specific Aim #3: Determine the functional activities and biologic relevance of novel SCR-containing candidate C3/C4 binding and regulatory proteins. ..
  44. C3D/EBV RECEPTOR LIGAND BINDING SITES
    V Holers; Fiscal Year: 2006
    ....
  45. Molecular Genetics of Drosophila MAGUK's
    Peter Bryant; Fiscal Year: 2005
    ..The work will provide a genetic approach to understanding the functions of Dlg and its mammalian homologs, some of which are strongly implicated in a variety of human cancers. ..
  46. Delineation of genetic pathways to lupus pathogenesis
    Kui Liu; Fiscal Year: 2003
    ..Sle1 mediates the loss of tolerance to nuclear antigens and the initiation of autoimmunity...
  47. Gender-Specific Role of p202 in Lupus Susceptibility
    Divaker Choubey; Fiscal Year: 2010
    ..Our studies will increase the understanding of the molecular basis of sex bias in lupus disease and could potentially lead to better diagnostic tools as well as disease monitoring, and effective treatments. 1 ..
  48. SIALOADENITIS IN AG INDUCED AND SPONTANEOUS AUTOIMMUNITY
    Shu Man Fu; Fiscal Year: 2002
    ..In the fourth specific aim, B-less mice which are congenic with the sialoadenitis susceptible strains will be generated to explore the role of B cells in both the spontaneous and antigen-induced autoimmune model. ..
  49. Pre-BCR expression level regulates cellular functions
    Stephen Clarke; Fiscal Year: 2002
    ..In the final aim whether the basis for the inability of 8/GO Tg mice to generate immature B cells is due to a pre-BCR or BCR deficiency will be determined. ..
  50. Comparative DNA Microarray Analysis of Brain Tumors
    Daniel Brat; Fiscal Year: 2006
    ....
  51. Metotic Spindle Assembly and Aneuploidy in Mammals
    Duane Compton; Fiscal Year: 2003
    ..abstract_text> ..
  52. Novel Image-Based Screening of Mammary Tumors
    David Knowles; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..