viral matrix proteins

Summary

Summary: Proteins associated with the inner surface of the lipid bilayer of the viral envelope. These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell.

Top Publications

  1. pmc Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1
    Tamara K Berthoud
    The Jenner Institute, Oxford University, Oxford, United Kingdom
    Clin Infect Dis 52:1-7. 2011
  2. pmc Influenza virus M2 protein mediates ESCRT-independent membrane scission
    Jeremy S Rossman
    Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA
    Cell 142:902-13. 2010
  3. pmc Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans
    Rebecca J Garten
    WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Science 325:197-201. 2009
  4. pmc Universal M2 ectodomain-based influenza A vaccines: preclinical and clinical developments
    Michael Schotsaert
    Department for Molecular Biomedical Research, Ghent University Technologpierpark 927, B 9052 Ghent, Belgium
    Expert Rev Vaccines 8:499-508. 2009
  5. ncbi Influenza A vaccine based on the extracellular domain of M2: weak protection mediated via antibody-dependent NK cell activity
    Andrea Jegerlehner
    Cytos Biotechnology, Schlieren, Switzerland
    J Immunol 172:5598-605. 2004
  6. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001
  7. ncbi Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellin
    James W Huleatt
    VaxInnate Corporation, 300 George Street, New Haven, CT 06511, USA
    Vaccine 26:201-14. 2008
  8. ncbi Phosphorylation and nuclear translocation of STAT3 regulated by the Epstein-Barr virus latent membrane protein 1 in nasopharyngeal carcinoma
    Yi Ping Liu
    Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan 410078, PR China
    Int J Mol Med 21:153-62. 2008
  9. pmc Lassa virus Z protein is a matrix protein and sufficient for the release of virus-like particles [corrected]
    Thomas Strecker
    Institut für Virologie der Philipps Universität Marburg, D 35037 Marburg, Germany
    J Virol 77:10700-5. 2003
  10. pmc Inhibition of Lassa and Marburg virus production by tetherin
    Toshie Sakuma
    First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277 0882, Japan
    J Virol 83:2382-5. 2009

Detail Information

Publications318 found, 100 shown here

  1. pmc Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1
    Tamara K Berthoud
    The Jenner Institute, Oxford University, Oxford, United Kingdom
    Clin Infect Dis 52:1-7. 2011
    ..A vaccine providing protective immunity to the highly conserved internal antigens could provide longer-lasting protection against multiple influenza subtypes...
  2. pmc Influenza virus M2 protein mediates ESCRT-independent membrane scission
    Jeremy S Rossman
    Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA
    Cell 142:902-13. 2010
    ..These data suggest that M2 mediates the final steps of budding for influenza viruses, bypassing the need for host ESCRT proteins...
  3. pmc Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans
    Rebecca J Garten
    WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Science 325:197-201. 2009
    ..Antigenically the viruses are homogeneous and similar to North American swine A(H1N1) viruses but distinct from seasonal human A(H1N1)...
  4. pmc Universal M2 ectodomain-based influenza A vaccines: preclinical and clinical developments
    Michael Schotsaert
    Department for Molecular Biomedical Research, Ghent University Technologpierpark 927, B 9052 Ghent, Belgium
    Expert Rev Vaccines 8:499-508. 2009
    ..Ultimately, and possibly in the near future, efficacy studies in humans should provide proof that this novel vaccine concept can mitigate epidemic and even pandemic influenza A virus infections...
  5. ncbi Influenza A vaccine based on the extracellular domain of M2: weak protection mediated via antibody-dependent NK cell activity
    Andrea Jegerlehner
    Cytos Biotechnology, Schlieren, Switzerland
    J Immunol 172:5598-605. 2004
    ....
  6. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001
    ..Consistent with this, NP bound CRM1 in vitro. Overall, these data raise the possibility that RNP export is mediated by a direct interaction between NP and the cellular CRM1 export pathway...
  7. ncbi Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellin
    James W Huleatt
    VaxInnate Corporation, 300 George Street, New Haven, CT 06511, USA
    Vaccine 26:201-14. 2008
    ..These data demonstrate that the linkage of specific TLR ligand with influenza M2e yields a vaccine candidate that offers significant promise for widespread protection against multiple influenza A virus strains...
  8. ncbi Phosphorylation and nuclear translocation of STAT3 regulated by the Epstein-Barr virus latent membrane protein 1 in nasopharyngeal carcinoma
    Yi Ping Liu
    Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan 410078, PR China
    Int J Mol Med 21:153-62. 2008
    ....
  9. pmc Lassa virus Z protein is a matrix protein and sufficient for the release of virus-like particles [corrected]
    Thomas Strecker
    Institut für Virologie der Philipps Universität Marburg, D 35037 Marburg, Germany
    J Virol 77:10700-5. 2003
    ..contains two late domains, PTAP and PPXY, necessary for the release of virus-like particles. Our data provide evidence that Z is the Lassa virus matrix protein that is the driving force for virus particle release...
  10. pmc Inhibition of Lassa and Marburg virus production by tetherin
    Toshie Sakuma
    First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277 0882, Japan
    J Virol 83:2382-5. 2009
    ..Here, we show that the production of virus-like particles induced by viral matrix proteins of Lassa virus or Marburg virus was markedly inhibited by tetherin and that N-linked glycosylation of ..
  11. pmc Mechanism of drug inhibition and drug resistance of influenza A M2 channel
    Rafal M Pielak
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:7379-84. 2009
    ..Therefore, it is present only when the helices are tightly packed in the closed conformation. Thus, drug-resistant mutants impair drug binding by destabilizing helix-helix assembly...
  12. ncbi B cell receptor signal strength determines B cell fate
    Stefano Casola
    CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 5:317-27. 2004
    ..Furthermore, spontaneous germinal centers developed in gut-associated lymphoid tissue of LMP2A mice, indicating that microbial antigens can promote germinal centers independently of BCR-mediated antigen recognition...
  13. ncbi The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family
    G Mosialos
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    Cell 80:389-99. 1995
    ..The interaction of LMP1 with these TNFR family-associated proteins is further evidence for their role in signaling and links LMP1-mediated transformation to signal transduction from the TNFR family...
  14. pmc Epstein-Barr virus-encoded LMP2A induces an epithelial-mesenchymal transition and increases the number of side population stem-like cancer cells in nasopharyngeal carcinoma
    Qing Li Kong
    State Key Laboratory of Oncology in South China, Sun Yat sen University Cancer Center, Guangzhou, People s Republic of China
    PLoS Pathog 6:e1000940. 2010
    ..Taken together, our data indicate that LMP2A induces EMT and stem-like cell self-renewal in NPC, suggesting a novel mechanism by which Epstein-Barr virus induces the initiation, metastasis and recurrence of NPC...
  15. ncbi Induction of influenza type A virus-specific resistance by immunization of mice with a synthetic multiple antigenic peptide vaccine that contains ectodomains of matrix protein 2
    Krystyna Mozdzanowska
    Immunology Program, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104 4268, USA
    Vaccine 21:2616-26. 2003
    ..Importantly, the protection in M2e-MAP- and infection-immunized mice appeared to be mediated by distinct immune mechanisms. This suggests that stronger protection may be achievable by combining both protective activities...
  16. pmc Sublingual immunization with M2-based vaccine induces broad protective immunity against influenza
    Byoung Shik Shim
    Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea
    PLoS ONE 6:e27953. 2011
    ..The mechanism whereby s.l. immunization with M2e vaccine candidate induces broad protection against infection with different influenza virus subtypes was explored...
  17. pmc Preliminary assessment of the efficacy of a T-cell-based influenza vaccine, MVA-NP+M1, in humans
    Patrick J Lillie
    Jenner Institute, University of Oxford, UK
    Clin Infect Dis 55:19-25. 2012
    ..Following a phase 1 clinical study that demonstrated vaccine safety and immunogenicity, a phase 2a vaccination and influenza challenge study has been conducted in healthy adult volunteers...
  18. ncbi Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells
    R Ghildyal
    Children s Virology Research Unit, Macfarlane Burnet Institute for Medical Research and Public Health, PO Box 254, Yarra Bend Road, Fairfield, Victoria 3078, Australia
    J Gen Virol 83:753-7. 2002
    ..These data suggest that the M protein is associated with RSV nucleocapsids and, like the matrix proteins of other negative-strand RNA viruses, can inhibit virus transcription...
  19. ncbi The accumulation of influenza A virus segment 7 spliced mRNAs is regulated by the NS1 protein
    Nicole C Robb
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    J Gen Virol 93:113-8. 2012
    ..These findings suggest that the NS1 protein plays a role in the regulation of splicing of influenza virus M1 mRNA...
  20. pmc Myristoylation of the RING finger Z protein is essential for arenavirus budding
    Mar Perez
    The Scripps Research Institute, IMM6, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Virol 78:11443-8. 2004
    ..These findings indicate N-terminal myristoylation of Z plays a key role in arenavirus budding...
  21. pmc Requirements for budding of paramyxovirus simian virus 5 virus-like particles
    Anthony P Schmitt
    Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 60208 3500, USA
    J Virol 76:3952-64. 2002
    ..Taken together, these data suggest that multiple viral components, including assembled nucleocapsids, have important roles in the paramyxovirus budding process...
  22. ncbi LMP1 TRAFficking activates growth and survival pathways
    Vishal Soni
    Channing Laboratory and Infectious Disease Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School and University, Boston, Massachusetts 02115, USA
    Adv Exp Med Biol 597:173-87. 2007
    ..Studies of the chemistry and biology of LMP1-TRAF interaction mediated activation of signaling pathways are important for controlling EBV infected cell survival and growth...
  23. pmc Dual resistance to adamantanes and oseltamivir among seasonal influenza A(H1N1) viruses: 2008-2010
    Tiffany G Sheu
    Influenza Division, Centers for Disease Control and Prevention, Battelle Memorial Institute, Atlanta, Georgia, USA
    J Infect Dis 203:13-7. 2011
    ..Because of limited options for antiviral treatment, emergence of dual-resistant influenza viruses poses a public health concern, and their circulation needs to be closely monitored...
  24. pmc Modulation of LMP1 protein expression by EBV-encoded microRNAs
    Angela Kwok Fung Lo
    Viral Oncology Program, Sidney Kimmel Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 104:16164-9. 2007
    ..The data provide insight into the discrepancy between LMP1 transcript and protein detection in NPC and highlight the role of the EBV miRNAs in regulating LMP1 downstream signaling to promote cancer development...
  25. ncbi Universal influenza A vaccine: optimization of M2-based constructs
    Marina De Filette
    DMBR, Ghent University VIB, FSVM Building, Ghent Zwijnaarde, Belgium
    Virology 337:149-61. 2005
    ..The most protective particle contained three consecutive M2e-copies linked to the N-terminus of HBc. Although HBc is highly immunogenic, the optimized M2e-HBc vaccine induced an anti-M2e antibody titer even higher than that of anti-HBc...
  26. pmc Influenza virus hemagglutinin and neuraminidase, but not the matrix protein, are required for assembly and budding of plasmid-derived virus-like particles
    Benjamin J Chen
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
    J Virol 81:7111-23. 2007
    ..VLP formation also occurred independently of the function of Vps4 in the multivesicular body pathway, as dominant-negative Vps4 proteins failed to inhibit influenza VLP budding...
  27. ncbi VSV disrupts the Rae1/mrnp41 mRNA nuclear export pathway
    Paula A Faria
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33136, USA
    Mol Cell 17:93-102. 2005
    ..Thus, these results demonstrate that VSV M protein blocks mRNA export by disrupting Rae1 function, which can be reverted by induction of Rae1 expression...
  28. pmc Quantitative analysis of Nipah virus proteins released as virus-like particles reveals central role for the matrix protein
    Jared R Patch
    Department of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland 20814, USA
    Virol J 4:1. 2007
    ..Here, we have established recombinant expression systems to study NiV particle assembly and budding through the formation of virus-like particles (VLPs)...
  29. ncbi A structural analysis of M protein in coronavirus assembly and morphology
    Benjamin W Neuman
    School of Biological Sciences, University of Reading, RG6 6AJ, UK
    J Struct Biol 174:11-22. 2011
    ..These findings provide insight into how M protein functions to promote virus assembly...
  30. pmc Insight into the mechanism of the influenza A proton channel from a structure in a lipid bilayer
    Mukesh Sharma
    Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA
    Science 330:509-12. 2010
    ..This mechanism explains the main observations on M2 proton conductance...
  31. pmc PRA1 promotes the intracellular trafficking and NF-kappaB signaling of EBV latent membrane protein 1
    Hao Ping Liu
    Institute of Microbiology and Immunology, National Yang Ming University, Shih Pai, Taipei, Taiwan, Republic of China
    EMBO J 25:4120-30. 2006
    ..These novel findings provide important new insights into LMP1, and identify an unexpected new role for PRA1 in cellular signaling...
  32. ncbi Assembly and budding of influenza virus
    Debi P Nayak
    Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA
    Virus Res 106:147-65. 2004
    ..Elucidation of both viral and host factors involved in viral morphogenesis and budding may lead to the development of drugs interfering with the steps of viral morphogenesis and in disease progression...
  33. pmc Cyclophilin A restricts influenza A virus replication through degradation of the M1 protein
    Xiaoling Liu
    Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    PLoS ONE 7:e31063. 2012
    ..Our results suggest that CypA restricts influenza virus replication through accelerating degradation of the M1 protein...
  34. ncbi Cell therapy of stage IV nasopharyngeal carcinoma with autologous Epstein-Barr virus-targeted cytotoxic T lymphocytes
    Patrizia Comoli
    Laboratorio di Ricerca Area Trapianti e Oncoematologia Pediatrica, IRCCS Policlinico S Matteo, V le Golgi 19, 27100 Pavia, Italy
    J Clin Oncol 23:8942-9. 2005
    ..We conducted a clinical trial of EBV-targeted cell therapy with autologous virus-specific cytotoxic T lymphocytes (CTLs) for NPC refractory to conventional treatments...
  35. ncbi Genetic control of immune responses to influenza A matrix 2 protein (M2)
    Julia A Misplon
    Food and Drug Administration, Center for Biologics and Research, HFM 730, Rockville, MD 20852, USA
    Vaccine 28:5817-27. 2010
    ..The conjugate vaccine enhanced some outcomes but not others. Co-immunizing with NP improved outcomes over either NP or M2 immunizations alone. These results have implications for vaccination of genetically diverse populations...
  36. pmc Induction of epidermal growth factor receptor expression by Epstein-Barr virus latent membrane protein 1 C-terminal-activating region 1 is mediated by NF-kappaB p50 homodimer/Bcl-3 complexes
    Natalie J Thornburg
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Virol 81:12954-61. 2007
    ..The distinct forms of NF-kappaB that are induced by LMP1 CTAR1 likely activate distinct cellular genes...
  37. ncbi Universal vaccine based on ectodomain of matrix protein 2 of influenza A: Fc receptors and alveolar macrophages mediate protection
    Karim El Bakkouri
    Department for Molecular Biomedical Research, Flanders Institute of Biotechnology VIB, B 9052 Ghent, Belgium
    J Immunol 186:1022-31. 2011
    ..We conclude that AM and Fc receptor-dependent elimination of influenza A virus-infected cells are essential for protection by anti-M2e IgG...
  38. pmc Structure of the amantadine binding site of influenza M2 proton channels in lipid bilayers
    Sarah D Cady
    Department of Chemistry, Iowa State University, Ames, Iowa 50011 2, USA
    Nature 463:689-92. 2010
    ..The study demonstrates the ability of solid-state NMR to elucidate small-molecule interactions with membrane proteins and determine high-resolution structures of their complexes...
  39. pmc Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions
    S L Zebedee
    Department of Biochemistry, Molecular Biology, Northwestern University, Evanston, Illinois 60208
    J Virol 62:2762-72. 1988
    ....
  40. ncbi M2e-based universal influenza A vaccine
    Walter Fiers
    Department for Molecular Biomedical Research, VIB, Ghent and Department for Molecular Biology, Ghent University, Technologiepark 927, B 9052 Ghent, Belgium
    Vaccine 27:6280-3. 2009
    ..Further clinical development of this universal influenza A vaccine candidate is being pursued in order to validate its protective efficacy in humans...
  41. ncbi Development of a universal influenza A vaccine based on the M2e peptide fused to the papaya mosaic virus (PapMV) vaccine platform
    Jérôme Denis
    Centre de Recherche en Infectiologie, Pavillon CHUL, Universite Laval, 2705 Boulevard Laurier, Quebec, PQ, G1V 4G2 Canada
    Vaccine 26:3395-403. 2008
    ..These results show, for the first time, the potential of a recombinant plant virus protein to serve as both peptide delivery system and adjuvant in the crucial field of influenza vaccine development...
  42. ncbi Role of the Epstein-Barr virus-encoded latent membrane protein-1, LMP1, in the pathogenesis of nasopharyngeal carcinoma
    Mhairi A Morris
    Cancer Research UK Institute for Cancer Studies, School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, UK
    Future Oncol 5:811-25. 2009
    ..Here we review the effects of LMP1 on epithelial cell growth transformation, and its putative role in the pathogenesis of nasopharyngeal carcinoma, focusing on key areas of proliferation, survival, cell motility and invasion...
  43. ncbi Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98
    - von Kobbe C
    European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117, Heidelberg, Germany
    Mol Cell 6:1243-52. 2000
    ..Together, our data demonstrate that VSV M inhibits host cell gene expression by targeting a nucleoporin and primarily blocking nuclear export...
  44. ncbi Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses
    Graeme E Price
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA
    Vaccine 27:6512-21. 2009
    ..These findings demonstrate potent protection by vaccination highly focused on conserved antigens and identify immune response measures in mice that differed among vaccinations and correlated with outcome...
  45. ncbi Twist and epithelial-mesenchymal transition are induced by the EBV oncoprotein latent membrane protein 1 and are associated with metastatic nasopharyngeal carcinoma
    Toshiyuki Horikawa
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Cancer Res 67:1970-8. 2007
    ..These results suggest that induction of Twist by a human viral oncoprotein LMP1 directly contributes to the metastatic nature of NPC...
  46. ncbi Differential signaling pathways are activated in the Epstein-Barr virus-associated malignancies nasopharyngeal carcinoma and Hodgkin lymphoma
    Jennifer A Morrison
    Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Cancer Res 64:5251-60. 2004
    ..These findings point to a divergence in pathways activated by EBV in different cellular contexts...
  47. pmc Role of matrix and fusion proteins in budding of Sendai virus
    T Takimoto
    Department of Virology and Molecular Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Virol 75:11384-91. 2001
    ..Our results indicate that both M and F proteins are able to drive the budding of SV and propose the possible role of actin in the budding process...
  48. pmc Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells
    Torsten Bunde
    Institut fur Medizinische Immunologie, Charite, Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    J Exp Med 201:1031-6. 2005
    ..In conclusion, high frequencies of IE-1 but not pp65-specific CD8 T cells correlate with protection from CMV disease. These results have important implications for monitoring T cell responses, adoptive cell therapy, and vaccine design...
  49. ncbi Epstein-Barr virus latent membrane protein 1 (LMP1) activates the phosphatidylinositol 3-kinase/Akt pathway to promote cell survival and induce actin filament remodeling
    Christopher W Dawson
    Cancer Research UK Institute for Cancer Studies, The University of Birmingham Medical School, United Kingdom
    J Biol Chem 278:3694-704. 2003
    ....
  50. ncbi Epstein-Barr virus: exploiting the immune system
    D A Thorley-Lawson
    Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Nat Rev Immunol 1:75-82. 2001
    ....
  51. pmc A positive autoregulatory loop of LMP1 expression and STAT activation in epithelial cells latently infected with Epstein-Barr virus
    Honglin Chen
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, Maryland 21231, USA
    J Virol 77:4139-48. 2003
    ..This in turn would establish a positive feedback loop of IL-6-induced STAT activation, LMP1 and Qp-EBNA1 expression, and viral genome persistence...
  52. ncbi The M1 matrix protein controls the filamentous phenotype of influenza A virus
    C J Elleman
    School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK
    Virology 321:144-53. 2004
    ..This observation suggests that an interaction among these regions of M1 may occur during assembly...
  53. pmc The extent of genetic diversity of Epstein-Barr virus and its geographic and disease patterns: a need for reappraisal
    Cindy M Chang
    Infections and Immunoepidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, 6120 Executive Blvd, EPS 7074, Rockville, MD 20892, USA
    Virus Res 143:209-21. 2009
    ....
  54. pmc Requirement for TRAF3 in signaling by LMP1 but not CD40 in B lymphocytes
    Ping Xie
    Department of Microbiology, University of Iowa, Iowa City 52242, USA
    J Exp Med 199:661-71. 2004
    ..Our data reveal that CD40 and LMP1 unexpectedly use TRAF3 in different ways, and that TRAF3 is required for LMP1-mediated activation of B cells...
  55. pmc Manipulation of the toll-like receptor 7 signaling pathway by Epstein-Barr virus
    Heather J Martin
    Johns Hopkins School of Medicine, Bunting Blaustein Building CRB308, 1650 Orleans Street, Baltimore, MD 21231 1000, USA
    J Virol 81:9748-58. 2007
    ..EBV therefore initially uses TLR7 signaling to enhance B-cell proliferation and subsequently modifies the pathway to regulate IRF-5 activity...
  56. pmc Influenza virus m2 ion channel protein is necessary for filamentous virion formation
    Jeremy S Rossman
    Department of Biochemistry, Molecular Biology, and Cell Biology, and Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 60208 3500, USA
    J Virol 84:5078-88. 2010
    ..We found that an amphipathic helix located within the M2 cytoplasmic tail is able to bind cholesterol, and we speculate that M2 cholesterol binding is essential for both filament formation and the stability of existing viral filaments...
  57. ncbi LMP1 structure and signal transduction
    A G Eliopoulos
    CRC Institute for Cancer Studies, The University of Birmingham Medical School, Birmingham, B15 2TA, UK
    Semin Cancer Biol 11:435-44. 2001
    ..The constitutive engagement of these signals and the characteristic molecular interactions that regulate them provide the basis for the molecular explanation of the transforming properties of this key EBV protein...
  58. pmc Designing inhibitors of M2 proton channel against H1N1 swine influenza virus
    Qi Shi Du
    Guangxi Academy of Sciences, Nanning, Guangxi, China
    PLoS ONE 5:e9388. 2010
    ..The NMR structure of the M2 channel protein determined by Schnell and Chou (Nature, 2008, 451, 591-595) may help people to solve the drug-resistant problem and develop more powerful new drugs against H1N1 influenza virus...
  59. ncbi Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle
    Ken Watanabe
    Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, Nagasaki University, 1 14 Bunkyo machi, Nagasaki 852 8521, Japan
    FEBS Lett 580:5785-90. 2006
    ..This is the first demonstration that Hsc70 is directly associated with M1 and therefore is required for viral production...
  60. ncbi Rapid identification and sorting of viable virus-reactive CD4(+) and CD8(+) T cells based on antigen-triggered CD137 expression
    Thomas C Wehler
    Department of Medicine III Hematology and Oncology, Johannes Gutenberg University of Mainz, Mainz, Germany
    J Immunol Methods 339:23-37. 2008
    ..The CD137 assay is most attractive for the simultaneous targeting of anti-viral T helper and effector cells in monitoring studies and adoptive immunotherapy trials...
  61. pmc Influenza C virus CM2 integral membrane glycoprotein is produced from a polypeptide precursor by cleavage of an internal signal sequence
    A Pekosz
    Howard Hughes Medical Institute, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
    Proc Natl Acad Sci U S A 95:13233-8. 1998
    ..Protein p31, in comparison to CM1, displays characteristics of an integral membrane protein...
  62. pmc Suppression of the Sendai virus M protein through a novel short interfering RNA approach inhibits viral particle production but does not affect viral RNA synthesis
    Geneviève Mottet-Osman
    Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CMU, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    J Virol 81:2861-8. 2007
    ..This level of suppression had no apparent effect on viral replication and transcription, supporting the lack of M involvement in SeV transcription or replication control...
  63. ncbi Nuclear traffic of influenza virus proteins and ribonucleoprotein complexes
    Sébastien Boulo
    Institut de Virologie Moléculaire et Structurale, FRE 2854 CNRS UJF, BP 181, 38042 Grenoble Cedex 9, France
    Virus Res 124:12-21. 2007
    ....
  64. ncbi Effective treatment of metastatic forms of Epstein-Barr virus-associated nasopharyngeal carcinoma with a novel adenovirus-based adoptive immunotherapy
    Corey Smith
    Australian Centre for Vaccine Development and Tumour Immunology Laboratory, Department of Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    Cancer Res 72:1116-25. 2012
    ..Taken together, our findings show that adoptive immunotherapy with AdE1-LMPpoly vaccine is safe and well tolerated and may offer clinical benefit to patients with NPC...
  65. pmc EBV latent membrane protein 1 activates Akt, NFkappaB, and Stat3 in B cell lymphomas
    Kathy H Y Shair
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Pathog 3:e166. 2007
    ..These results suggest that Akt, NFkappaB, and Stat3 pathways may serve as effective targets in the treatment of EBV-associated B cell lymphomas...
  66. pmc The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL
    M R Wills
    Department of Medicine, University of Cambridge Clinical School, United Kingdom
    J Virol 70:7569-79. 1996
    ....
  67. ncbi Molecular interactions in the assembly of coronaviruses
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
    Adv Virus Res 64:165-230. 2005
  68. pmc Structure and mechanism of the M2 proton channel of influenza A virus
    Jason R Schnell
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 451:591-5. 2008
    ..Drug-resistance mutations are predicted to counter the effect of drug binding by either increasing the hydrophilicity of the pore or weakening helix-helix packing, thus facilitating channel opening...
  69. pmc Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production
    Yue Huang
    Vaccine Research Center, Natiuonal Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, MD 20982 3005, USA
    J Virol 78:12557-65. 2004
    ..These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs...
  70. pmc The morphology and composition of influenza A virus particles are not affected by low levels of M1 and M2 proteins in infected cells
    Svetlana V Bourmakina
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, Box 1124, New York, NY 10029, USA
    J Virol 79:7926-32. 2005
    ....
  71. pmc Mutations at alternative 5' splice sites of M1 mRNA negatively affect influenza A virus viability and growth rate
    Chiayn Chiang
    Research Center for Emerging Viral Infections, Chang Gung University, Kwei Shan, Taoyuan, Taiwan
    J Virol 82:10873-86. 2008
    ..Results obtained here suggest that introducing these mutations into the alternative 5' splice sites disturbed M1 mRNA splicing, which may attenuate viral growth rates...
  72. pmc A phase II study evaluating the safety and efficacy of an adenovirus-ΔLMP1-LMP2 transduced dendritic cell vaccine in patients with advanced metastatic nasopharyngeal carcinoma
    W K Chia
    Department of Medical Oncology, National Cancer Centre, Singapore
    Ann Oncol 23:997-1005. 2012
    ....
  73. pmc Viruslike particle vaccine induces protection against respiratory syncytial virus infection in mice
    Fu Shi Quan
    Zetra Biologicals LLC
    J Infect Dis 204:987-95. 2011
    ..Despite decades of research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches are therefore urgently needed to develop effective RSV vaccines...
  74. pmc A major determinant for membrane protein interaction localizes to the carboxy-terminal domain of the mouse coronavirus nucleocapsid protein
    Kelley R Hurst
    David Axelrod Institute, Wadsworth Center, NYSDOH, New Scotland Avenue, P O Box 22002, Albany, NY 12201 2002, USA
    J Virol 79:13285-97. 2005
    ..Further, we found that fusion of domain 3 of the N protein to the carboxy terminus of a heterologous protein caused it to be incorporated into MHV virions...
  75. ncbi Selection of T cell clones expressing high-affinity public TCRs within Human cytomegalovirus-specific CD8 T cell responses
    Lydie Trautmann
    Institut National de la Santé et de la Recherche Médicale INSERM Unité 601, Nantes, France
    J Immunol 175:6123-32. 2005
    ....
  76. ncbi DNAzymes targeted to EBV-encoded latent membrane protein-1 induce apoptosis and enhance radiosensitivity in nasopharyngeal carcinoma
    Zhong Xin Lu
    Cancer Research Institute, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan 410078, PR China
    Cancer Lett 265:226-38. 2008
    ..The results suggest that LMP1 may represent a molecular target for DNAzymes and provide a basis for the use of the LMP1 DNAzymes as potential radiosensitizers for treatment of the EBV-associated carcinomas...
  77. ncbi [Impact of up-regulating Ezrin expression by Epstein-Barr virus latent membrane protein 1 on metastasis ability of nasopharyngeal carcinoma cells]
    Zhi Hua Shen
    Department of Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong, 524023, P R China
    Ai Zheng 27:165-9. 2008
    ..This study was to investigate whether EBV-LMP1 could change the expression of Ezrin and the role of Ezrin on the metastasis of NPC cells...
  78. ncbi Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV)
    Qisheng Zheng
    Key Laboratory of Animal Disease Diagnosis and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China
    Virus Genes 35:585-95. 2007
    ..The strategy of co-expressing PRRSV GP5 and M protein in MVA under the control of different promoters might be an attractive method for future PRRSV vaccine design...
  79. ncbi Identification of a second protein encoded by influenza C virus RNA segment 6
    S Hongo
    Department of Bacteriology, Yamagata University School of Medicine, Japan
    J Gen Virol 75:3503-10. 1994
    ..Immunoprecipitation experiments with this antiserum revealed that a previously unrecognized protein of apparent M(r) approximately 18,000, designated CM2, is synthesized in influenza C virus-infected cells...
  80. pmc Genetic evidence for a structural interaction between the carboxy termini of the membrane and nucleocapsid proteins of mouse hepatitis virus
    Lili Kuo
    Wadsworth Center, New York State Department of Health, Albany, New York 12201 2002, USA
    J Virol 76:4987-99. 2002
    ..In addition, this work demonstrates the efficacy of targeted recombination with fMHV for the systematic genetic analysis of coronavirus structural protein interactions...
  81. pmc Matrix protein 2 vaccination and protection against influenza viruses, including subtype H5N1
    Stephen Mark Tompkins
    Food and Drug Administration, Bethesda, Maryland, USA
    Emerg Infect Dis 13:426-35. 2007
    ..This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A...
  82. pmc Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) and LMP2A function cooperatively to promote carcinoma development in a mouse carcinogenesis model
    Kathy H Y Shair
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 86:5352-65. 2012
    ..This study is the first to characterize the effects of LMP2 on tumor initiation and promotion and to identify an effect of the combined expression of LMP1 and LMP2 on the increase of carcinoma development...
  83. ncbi Research and development of universal influenza vaccines
    Lanying Du
    Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10065, USA
    Microbes Infect 12:280-6. 2010
    ..This review describes the advancements in the research and development of universal influenza vaccines based on the relatively conserved sequences of M2e, HA, and other proteins of influenza viruses...
  84. pmc Epstein-Barr virus in Burkitt's lymphoma: a role for latent membrane protein 2A
    Kathryn T Bieging
    Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA
    Cell Cycle 9:901-8. 2010
    ..This work uncovers a contribution of EBV to molecular events that have documented importance in BL pathogenesis, and may underlie the poorly understood link between EBV and BL...
  85. ncbi A functional link between the actin cytoskeleton and lipid rafts during budding of filamentous influenza virions
    Martha Simpson-Holley
    Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
    Virology 301:212-25. 2002
    ..We propose that the actin cytoskeleton is required to maintain the correct organisation of lipid rafts for incorporation into budding viral filaments...
  86. pmc The influenza A virus PB2, PA, NP, and M segments play a pivotal role during genome packaging
    Qinshan Gao
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 86:7043-51. 2012
    ..These results indicate that the PB2, PA, NP, and M segments play a more important role than the remaining four vRNAs during the genome-packaging process...
  87. ncbi Epstein-Barr virus latent membrane protein 1 induces the chemotherapeutic target, thymidine phosphorylase, via NF-kappaB and p38 MAPK pathways
    Chia Chun Chen
    Graduate Institute of Biomedical Sciences, Chang Gung University, 259, Wen Hwa 1st Road, Kwei Shan, Taoyuan 333, Taiwan
    Cell Signal 22:1132-42. 2010
    ..Our results provide new insights into the roles of LMP1-mediated NF-kappaB and p38 MAPK signaling pathways in TP induction, potentially suggesting new therapeutic strategies for the treatment of NPC...
  88. pmc The Epstein-Barr virus (EBV)-induced tumor suppressor microRNA MiR-34a is growth promoting in EBV-infected B cells
    Eleonora Forte
    Department of Molecular Genetics and Microbiology, Center for Virology, Duke University Medical Center, Durham, North Carolina, USA
    J Virol 86:6889-98. 2012
    ..This study identifies a progrowth role for a tumor-suppressive miRNA in oncogenic-virus-mediated transformation, highlighting the importance of studying miRNA function in different cellular contexts...
  89. pmc Epstein-Barr latent membrane protein 1 transformation site 2 activates NF-kappaB in the absence of NF-kappaB essential modifier residues 133-224 or 373-419
    Daniela Boehm
    Department of Medicine, Division of Infectious Diseases, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:18103-8. 2010
    ..These data provide further evidence of the essential role for NEMO in LMP1 TES2 NF-κB activation and highlight the importance of unique domains within NEMO for sensing distinct NF-κB stimuli...
  90. pmc Bortezomib induces apoptosis of Epstein-Barr virus (EBV)-transformed B cells and prolongs survival of mice inoculated with EBV-transformed B cells
    Ping Zou
    Laboratory of Clinical Infectious Diseases, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    J Virol 81:10029-36. 2007
    ..These findings suggest that bortezomib may represent a novel strategy for the treatment of certain EBV-associated lymphomas...
  91. ncbi Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection
    J W Gratama
    Department of Clinical and Tumor Immunology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Blood 98:1358-64. 2001
    ..002). Thus, enumeration of HLA-restricted, CMV-specific CD8+ T cells in the grafts and monitoring of these T cells after SCT may constitute a rapid and sensitive tool to identify SCT recipients at risk for developing CMV disease...
  92. pmc Virus-like particle vaccine protects against 2009 H1N1 pandemic influenza virus in mice
    Fu Shi Quan
    Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, United States of America
    PLoS ONE 5:e9161. 2010
    ..The 2009 influenza pandemic and shortages in vaccine supplies worldwide underscore the need for new approaches to develop more effective vaccines...
  93. pmc A novel latent membrane 2 transcript expressed in Epstein-Barr virus-positive NK- and T-cell lymphoproliferative disease encodes a target for cellular immunotherapy
    Christopher P Fox
    School of Cancer Sciences, University of Birmingham, Birmingham, United Kingdom
    Blood 116:3695-704. 2010
    ..This study is registered at clinicaltrials.gov as NCT00062868...
  94. pmc Nipah virus-associated encephalitis outbreak, Siliguri, India
    Mandeep S Chadha
    National Institute of Virology, Pune, India
    Emerg Infect Dis 12:235-40. 2006
    ..NiV infection has not been previously detected in India...
  95. ncbi Association of the Epstein-Barr virus latent membrane protein 1 with lipid rafts is mediated through its N-terminal region
    S Rothenberger
    Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland
    Cell Mol Life Sci 59:171-80. 2002
    ..Moreover, Ndelta25 inhibited WT LMP1-mediated induction of the transcription factors NF-kappaB and AP-1. Morphological data indicate that Ndelta25 hampers WT LMP1 plasma membrane localization, thus blocking LMP1 function...
  96. pmc Epstein-Barr virus nuclear protein 2 mutations define essential domains for transformation and transactivation
    J I Cohen
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    J Virol 65:2545-54. 1991
    ..Furthermore, these results indicate that the transformation and transactivation functions of EBNA-2 may not be separable...
  97. ncbi Expression of immune-related molecules in primary EBV-positive Chinese nasopharyngeal carcinoma: associated with latent membrane protein 1 (LMP1) expression
    Jiang Li
    State Key Laboratory of Oncology in South China, Sun Yat Sen University, Cancer Center, Guangzhou, China
    Cancer Biol Ther 6:1997-2004. 2007
    ..It has been reported that the expression pattern of immune related-molecules on tumor cells will affect the outcome of T-cell-based adoptive immunotherapy for NPC...
  98. ncbi Specific latent membrane protein 1 gene sequences in type 1 and type 2 Epstein-Barr virus from nasopharyngeal carcinoma in Hong Kong
    S T Cheung
    Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin
    Int J Cancer 76:399-406. 1998
    ..Nonetheless, the 2 LMP1 variants showed differences in nucleotide alterations and were associated with different EBV types, suggesting the presence of 2 distinct EBV strains in Hong Kong NPC...
  99. ncbi Identification of an H-2D(b)-restricted CD8+ cytotoxic T lymphocyte epitope in the matrix protein of respiratory syncytial virus
    John A Rutigliano
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 2502, 40 Convent Drive, MSC 3017, Bethesda, MD 20892 3017, USA
    Virology 337:335-43. 2005
    ..Furthermore, identification of this H-2b-restricted CTL epitope provides access to genetically modified H-2b mice for more detailed studies of CTL mechanisms in RSV infection...
  100. ncbi The generation and characterization of LMP2-specific CTLs for use as adoptive transfer from patients with relapsed EBV-positive Hodgkin disease
    Catherine M Bollard
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, Texas 77030, USA
    J Immunother 27:317-27. 2004
    ..This approach is being used in a current clinical trial...
  101. pmc Influenza type A virus escape mutants emerge in vivo in the presence of antibodies to the ectodomain of matrix protein 2
    Darya Zharikova
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104 4268, USA
    J Virol 79:6644-54. 2005
    ..The implications of these findings for the use of M2e as a broadly protective vaccine are discussed...

Research Grants66

  1. Biodefense vaccine for pandemic influenza
    Lawrence Smith; Fiscal Year: 2005
    ..FINAL MILESTONE 3: Completion of Aim 2 and completion of IND ..
  2. Lipid-Protein Interactions in Viral Assembly and Virus Like Particle Formation
    ROBERT VIRGIL STAHELIN; Fiscal Year: 2012
    ..Moreover, from studying the viral matrix proteins much new information can be obtained on mechanisms of peripheral protein assembly impacting more than one ..
  3. Center for Membrane Proteins in Infectious Diseases (MPID)
    Petra Fromme; Fiscal Year: 2013
    ..The MPID center will also collaborate with the community and the PSI on the structure determination of target membrane proteins that complement the initial targets of the center. ..
  4. Membrane Control of Protein-Protein Contact. Simulation Based on Phase Diagrams
    GERALD WILLIAM FEIGENSON; Fiscal Year: 2010
    ..goal is to discover which membrane factors exert control over the interactions between membrane-associated viral matrix proteins. The overall approach is to combine experimental measurements, mainly FRET and fluorescence microscopy, ..
  5. Cellular Immunity to BK Virus
    Simon Lacey; Fiscal Year: 2005
    ..Such information may be useful in guiding clinical management, and lay the foundation for possible future vaccines. ..
  6. Criteria for Protective Immunity to BK virus in Kidney Transplant Recipients
    Simon Lacey; Fiscal Year: 2007
    ..Information from the proposed study may allow identification of patients at risk of progression from BKV reactivation to clinical BKV disease and guide therapeutic interventions. [unreadable] [unreadable] [unreadable]..
  7. EBV PATHOGENESIS IN HIV-ASSOCIATED HAIRY LEUKOPLAKIA
    DENNIS WALLING; Fiscal Year: 2003
    ..The results of this study will elucidate molecular mechanisms of EBV pathogenesis in HLP applicable to other EBV-associated diseases, including nasopharyngeal carcinoma and HIV-associated lymphomas. ..
  8. EBV and HPV: Oral Infection, Persistence & Pathogenesis
    DENNIS WALLING; Fiscal Year: 2003
    ..abstract_text> ..
  9. Modification of Conserved Influenza Genes Enabling their Elevated Expression
    Petr Ilyinskii; Fiscal Year: 2008
    ..Therefore, if proven, our approach will lead to a novel paradigm for recombinant gene modifications for enhanced expression in vivo, both for vaccine and non-vaccine applications. [unreadable] [unreadable] [unreadable]..
  10. Novel broad spectrum vaccination against influenza
    Petr Ilyinskii; Fiscal Year: 2006
    ..The overall aim of this project is to demonstrate better protective effect or/and enhanced immune response using modified influenza proteins as immunogens compared to wild-type viral proteins. ..
  11. Aberrant T Cell Function and Immunopathogenesis of CMV Immune Recovery Uveitis
    Mark Jacobson; Fiscal Year: 2008
    ..Thus, understanding the disease mechanism of CMV IRU could facilitate development of effective therapy for CMV IRU or diagnostic or treatment strategies to prevent CMV IRU. [unreadable] [unreadable] [unreadable]..
  12. Adjunct rhIL-12 enhance THI response to viral vaccine?
    Mark Jacobson; Fiscal Year: 2004
    ..g. CMV, HSV and HIV). ..
  13. CMV Immune Response & Long-Term Outcome of CMV Retinitis
    Mark Jacobson; Fiscal Year: 2005
    ....
  14. HIV Epitope Specific T Cell Responses and Control of HIV Replication
    Mark Jacobson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  15. High Throughput Assays for Ion Channel Activities of Influenza A & B Viruses
    LAWRENCE PINTO; Fiscal Year: 2006
    ..These assays will make it possible to perform efficient searches for antiviral drugs against the M2 ion channels. [unreadable] [unreadable] [unreadable] [unreadable]..
  16. Membrane Protein Structural Genomics: M. tuberculosis
    Timothy Cross; Fiscal Year: 2005
    ..More importantly, it would be a major step forward in characterizing membrane protein folds and would represent a treasure trove of structural information for combating this deadly organism. ..
  17. Tumor-Associated Herpesviruses Conference
    Nancy Raab Traub; Fiscal Year: 2006
    ..Recognize and encourage young investigators and 3. Highlight new developments in the field and identify areas for future investigation. ..
  18. Novel Strategy for Animal Model Testing of HCMV Vaccines
    Mark Schleiss; Fiscal Year: 2007
    ..These studies will represent the first efficacy test of a human CMV subunit vaccine in a small animal model of congenital CMV infection. [unreadable] [unreadable]..
  19. AIDS ASSOCIATED LEUKOPLAKIA--ROLE OF EBV
    Nancy Raab Traub; Fiscal Year: 2004
    ..abstract_text> ..
  20. Oncogenic properties of interferon regulatory factor 7
    Luwen Zhang; Fiscal Year: 2009
    ..This study should provide an insight of how IRF-7 participates in EBV transformation, and expand our knowledge about IRFs as well as the pathogenesis of EBV-associated CNS lymphoma. ..
  21. VACCINES FOR PREVENTION OF EXPERIMENTAL CONGENITAL CMV
    Mark Schleiss; Fiscal Year: 2009
    ..We anticipate that these subunit vaccine studies in this small animal model will clarify which strategies may be of value for vaccination against congenital HCMV infection. ..
  22. TRAF Regulation and Receptor Interactions in TNFR Family Signals
    GAIL BISHOP; Fiscal Year: 2009
    ..To explore the role of TRAF5 in B cell activation. ..
  23. Studies on Influenza B Virus BM2 Protein Ion Channel
    LAWRENCE PINTO; Fiscal Year: 2009
    ....
  24. RESPIRATORY VIRUS ION CHANNELS
    LAWRENCE PINTO; Fiscal Year: 2007
    ..5. In a separate set of experiments we will use a sensitive method to test the NB protein of influenza B virus for ion channel activity. [unreadable] [unreadable] [unreadable]..
  25. Interferon Regulatory Factor 7 and NPC
    Luwen Zhang; Fiscal Year: 2006
    ..This study would lead to a better understanding of how LMP-1 and IRF-7 interact to participate in viral pathogenesis, latency, and transformation. ..
  26. Respiratory Viruses and Airflow Obstruction after HCT
    Michael Boeckh; Fiscal Year: 2008
    ..These studies will improve our understanding of the interaction between respiratory viruses and late pulmonary complications after HCT, which will ultimately enable us to formulate more rational treatment strategies. ..
  27. Epstein-Barr Virus: Activation of WNT
    Nancy Raab Traub; Fiscal Year: 2008
    ..A detailed understanding of the activation of this pathway may provide new opportunities for treatment of EBV-associated cancers and also of similar cancers considered EBV-negative, that develop in the immunosuppressed. ..
  28. Transgenic Plant-Derived CMV Glycoprotein B Vaccine
    Mark Schleiss; Fiscal Year: 2007
    ..These studies will advance the fields of plant and CMV vaccines. [unreadable] [unreadable] [unreadable]..
  29. Modulation of Apoptosis by IRF-4 in EBV transformation
    Luwen Zhang; Fiscal Year: 2010
    ..These experiments may identify the apoptosis. These experiments may identify critical mechanisms that IRF-4 uses to prevent apoptosis in EBV-transformed cells. ..
  30. CLONING AND EXPRESSION OF INFLUENZA VIRAL RNA SEGMENTS
    DEBI NAYAK; Fiscal Year: 2005
    ..A detailed understanding of these processes will facilitate the rational development of antiviral agents which could interfere with one or more steps in virus assembly. ..
  31. Determining the Conformation of the Influenza A M2 Protein using EPR Spectroscopy
    Kathleen Howard; Fiscal Year: 2008
    ..The M2 channel is pH-gated and the spin-labeled protein will be examined at several pH values to probe for conformational changes related to channel gating. [unreadable] [unreadable] [unreadable]..
  32. VACCINATION FOR CMV IN AIDS PATIENTS UNDERGOING HAART
    Don Diamond; Fiscal Year: 2001
    ..1-restricted CMV pp65 lipopeptide vaccine in patient volunteers. ..
  33. Proteomics to Biomimetic Polymers: Engineering Principle
    WILLIAM DEGRADO; Fiscal Year: 2002
    ..abstract_text> ..
  34. Altered CD8+ T cell Compartment in SLE Patients
    JACQUES F BANCHEREAU; Fiscal Year: 2010
    ..These studies will help us conclude whether killer CD8+T cells and/or suppressor CD8+ T cells i) can be used as markers and/or predictors of disease activity, ii) represent a valuable new option in lupus therapy. ..
  35. Dendritic Cells, Heat Shock Proteins and Tumor Immunity
    Jacques Banchereau; Fiscal Year: 2004
    ..These studies will permit us to produce an altered melanoma cell line, with enhanced immunogenicity, that will be used to generate tumor cell bodies to load onto DCs for vaccination protocols. ..
  36. EBOLA VIRUS INTERFERON-ANTAGONISTS AND VIRULENCE
    CHRISTOPHER BASLER; Fiscal Year: 2003
    ..VP35 and VP24 may also prove to be attractive targets for new antiviral agents. Inhibition of anti-IFN factors may sensitize Ebola virus infected cells to the antiviral effects of IFN and may also enhance the effect of antiviral agents. ..
  37. Avian Influenza Surveillance in Thailand-Studies at Human-Animal Interface
    Pilaipan Puthavathana; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  38. Defining Molecular Markers for Tumor Hypoxia
    QUYNH THU XUAN LE; Fiscal Year: 2010
    ..abstract_text> ..
  39. DE NOVO DESIGN OF ALPHA HELICAL BUNDLES
    WILLIAM DEGRADO; Fiscal Year: 2003
    ..In particular, proteins that bind dinuclear zinc, manganese, and iron will be prepared, and the influence of the protein matrix on the physical and chemical properties of the metal ions will be characterized. ..
  40. ROLE OF CD4+ TH1 LYMPHOCYTES IN PROTECTION AGAINST EIAV
    Travis McGuire; Fiscal Year: 2002
    ....
  41. DEVELOPMENT OF A LIVE ATTENUATED HSV-2 VACCINE
    Mark Prichard; Fiscal Year: 2002
    ..In addition further in vitro characterization will enable high quality clinical trial material to be produced for IND enabling primate studies and early Phase I human trials. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  42. BIOORGANIC MODELS FOR TRANSMEMBRANE PROTEINS
    WILLIAM DEGRADO; Fiscal Year: 2009
    ..Aim 3 We will structurally characterize water-soluble versions of phospholamban and KcsA. Aim4. We will develop computational methods for transmembrane protein structure prediction &design. ..
  43. SUBPOPULATIONS OF HUMAN DENDRITIC CELLS
    Jacques Banchereau; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  44. CONTROL OF PARAMYXOVIRUS RNA SYNTHESIS
    GRIFFITH PARKS; Fiscal Year: 2008
    ..Our work will increase our understanding of important virus-host cell interactions, as well as provide new approaches to improve the safety and potency of vaccine vectors. [unreadable] [unreadable] [unreadable]..
  45. Structure and Assembly of KSHV Capsids and Virions
    Z Zhou; Fiscal Year: 2006
    ..The results of these experiments will lead to a better understanding of the process of KSHV lytic replication and the identification of potential targets for therapeutic intervention. ..
  46. VIRUSES AND AUTOIMMUNE DISEASE
    Michael Oldstone; Fiscal Year: 2001
    ..The second order of business is to determine the role of "self" (islet-cell) antigens other than the initial triggering viral antigen in the pathogenetic process leading to and determine their role in IDDM. ..
  47. Budding of Negative-sense RNA Viruses
    RONALD HARTY; Fiscal Year: 2006
    ....
  48. Proteomics to Biomimetic Polymers: Engineering Principle
    WILLIAM DEGRADO; Fiscal Year: 2005
    ..abstract_text> ..
  49. MOLECULAR AND ONCOLOGIC VIROLOGY TRAINING PROGRAM
    Daniel DiMaio; Fiscal Year: 2005
    ..In these capacities, he is well positioned to foster interactions among other members of the faculty in the School of Medicine. ..
  50. CMV Vaccines: Reinfection and Antigenic Variation
    William Britt; Fiscal Year: 2007
    ..These studies are essential for the development of a vaccine which will protect offspring born to women at risk for infection, especially women at risk for reinfection. ..
  51. The Response to Influenza: Aging and Vaccine Efficacy
    Yuping Deng; Fiscal Year: 2005
    ..Information from this study will also have great implication on strategy for protecting elderly population against other bioterrorist attack. ..
  52. Therapeutic Vaccine for EBV-associated Hodgkins Disease
    Rajiv Khanna; Fiscal Year: 2005
    ..The long-term aim of this project is to develop a rational basis for the delivery of a curative EBV-specific CTL immunity to not only in HIV patients with HD patients but also for non-HIV HD. ..
  53. CNS MALDEVELOPMENT AND PERINANTAL INFECTION
    William Britt; Fiscal Year: 2007
    ..These studies will provide a greater understanding of the pathogenesis of perinatal infections and could lead to improved therapy of these potentially treatable causes of mental retardation. ..
  54. INTERFERENCE BY DEFECTIVE INFLUENZA VIRUSES
    DEBI NAYAK; Fiscal Year: 2007
    ..abstract_text> ..
  55. Role of cdks and dynein in HCMV Assembly
    Veronica Sanchez; Fiscal Year: 2007
    ..In addition, the results from these studies will provide the basis for Dr. Sanchez to develop an independent research program and assist her to secure a faculty position at a university or medical school. ..
  56. Mycobacterium tuberculosis-reactive thymocytes
    MARIELLE GOLD; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  57. EIAV vector targeting dendritic cells to induce CTL
    ROBERT MEALEY; Fiscal Year: 2005
    ..Thus, this proposal will test the hypothesis that targeting dendritic cells in vivo with an EIAV vector will induce CTL in horses to multiple viral proteins. ..
  58. Analysis of the Ebola Virus VP24 Channel Protein
    RONALD HARTY; Fiscal Year: 2003
    ..Overall this application will address key mechanisms of Ebola virus pathogenesis that may lead to the development of novel paradigms with direct impact on the development of therapeutic approaches. ..
  59. UL34 and herpes simplex virus envelopment
    Richard J Roller; Fiscal Year: 2010
    ..3. We will fully characterize changes in the host cell nuclear lamina associated with infection and determine the mechanism by which UL34 and other viral proteins accomplish those changes. ..
  60. Viruses and Diabetes
    Michael Oldstone; Fiscal Year: 2005
    ....
  61. Aging Disease--Prion/Transgenic/Immunologic Studies
    Michael Oldstone; Fiscal Year: 2008
    ..Lastly, Jose Criado will continue his electrophysiology and behavioral studies on the normal function of PrP and on its aberrations during TSE disease. ..
  62. Arenavirus Receptor-Structure/Function Studies
    Michael B Oldstone; Fiscal Year: 2010
    ..These findings have relevance for biodefense of several viruses including LFV. ..
  63. Pathogenesis Studies in Scrapie (TSE Diseases)
    Michael Oldstone; Fiscal Year: 2003
    ..This model should be useful to study pathophysiology of CWD as well as probing CWD strain differences. ..
  64. Assembly and release of the SARS coronavirus
    Carolyn Machamer; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  65. LSRII Four Laser Analytical Flow Cytometer
    David Thorley Lawson; Fiscal Year: 2006
    ..This renders the acquisition of a new LSR II essential, if we are to meet the flow cytometry needs, both in time and available technology, for the community of NIH funded researchers at TUSM and T-NEMC. [unreadable] [unreadable]..