- Binding of hepatitis C virus to CD81
IRIS, Chiron, Siena 53100, Italy
Science 282:938-41. 1998
..Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro...
- Viral membrane fusion
Stephen C Harrison
Jack and Eileen Connors Structural Biology Laboratory, Harvard Medical School, Laboratory of Molecular Medicine, Children s Hospital Boston, Howard Hughes Medical Institute, 250 Longwood Avenue, Boston, MA 02115, USA
Nat Struct Mol Biol 15:690-8. 2008
..Fusion inhibitors can be effective antiviral agents...
- Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras
Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 103:7408-13. 2006
..Finally, we describe an E1-specific antiserum capable of neutralizing infectivity of all HCV chimeras...
- Cross-reacting antibodies enhance dengue virus infection in humans
Department of Medicine, Imperial College London, London W12 0NN, UK
Science 328:745-8. 2010
- Structure of dengue virus: implications for flavivirus organization, maturation, and fusion
Richard J Kuhn
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Cell 108:717-25. 2002
..The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane...
- The human immune response to Dengue virus is dominated by highly cross-reactive antibodies endowed with neutralizing and enhancing activity
Institute for Research in Biomedicine, Bellinzona 6500, Switzerland
Cell Host Microbe 8:271-83. 2010
..Our findings reveal an unexpected degree of cross-reactivity in human antibodies against DENV and illustrate the potential for an antibody-based therapy to control severe dengue...
- Complete replication of hepatitis C virus in cell culture
Brett D Lindenbach
Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Science 309:623-6. 2005
..HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals...
- Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Science 308:1643-5. 2005
..CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs...
- Identification of human neutralizing antibodies that bind to complex epitopes on dengue virions
Ruklanthi de Alwis
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
Proc Natl Acad Sci U S A 109:7439-44. 2012
..These results have significant implications for the DENV Ab and vaccine field...
- A structural perspective of the flavivirus life cycle
Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
Nat Rev Microbiol 3:13-22. 2005
..This review focuses mainly on structural studies of dengue virus...
- Hepatitis C virus entry depends on clathrin-mediated endocytosis
Equipe Hepatite C, CNRS UMR8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
J Virol 80:6964-72. 2006
..These data indicate that HCV enters target cells by clathrin-mediated endocytosis, followed by a fusion step from within an acidic endosomal compartment...
- Efficacy results of a trial of a herpes simplex vaccine
Robert B Belshe
Division of Infectious Diseases, Allergy, and Immunology, Saint Louis University, St Louis, MO 63104, USA
N Engl J Med 366:34-43. 2012
..Efficacy was not observed in men or HSV-1 seropositive women...
- Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate
Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA
Nat Med 3:866-71. 1997
..Similar motifs were also identified in the envelope proteins of other flaviviridae. Developing pharmaceuticals that inhibit target cell binding may be an effective strategy for treating flavivirus infections...
- Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus
Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, Missouri 63110, USA
Nat Med 11:522-30. 2005
..In postexposure therapeutic trials in mice, a single dose of humanized E16 protected mice against WNV-induced mortality, and may therefore be a viable treatment option against WNV infection in humans...
- Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States
R S Lanciotti
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA
Science 286:2333-7. 1999
..This North American WN virus was most closely related to a WN virus isolated from a dead goose in Israel in 1998...
- Coreceptor usage of primary human immunodeficiency virus type 1 isolates varies according to biological phenotype
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden
J Virol 71:7478-87. 1997
..The results show that all 36 primary HIV-1 isolates induce syncytia, provided that target cells carry the particular coreceptor required by the virus...
- Tracking global patterns of N-linked glycosylation site variation in highly variable viral glycoproteins: HIV, SIV, and HCV envelopes and influenza hemagglutinin
Theoretical Biology Group, Los Alamos National Laboratory, Los Alamos, NM 87544, USA
Glycobiology 14:1229-46. 2004
..Human influenza A hemagglutinin H3 HA1 is accumulating sequons over time, but this trend is not evident in any other avian or human influenza A serotypes...
- A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA
Nat Med 16:334-8. 2010
..Immunization with alphavirus VLP vaccines represents a strategy to contain the spread of CHIKV and related pathogenic viruses in humans...
- Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor
Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Institut Fédératif de Recherche 128, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 278:41624-30. 2003
..Finally, by correlating expression of HCV receptors and infectivity, we suggest that, besides CD81 and SR-B1, additional hepatocyte-specific co-factor(s) are necessary for HCV entry...
- Human monoclonal antibodies to a novel cluster of conformational epitopes on HCV E2 with resistance to neutralization escape in a genotype 2a isolate
Zhen Yong Keck
Department of Pathology, Stanford University School of Medicine, Stanford, California, United States of America
PLoS Pathog 8:e1002653. 2012
..Thus, the cluster of HC-84 epitopes, designated as antigenic domain D, is relevant for vaccine design for this highly diverse virus...
- Repulsion of superinfecting virions: a mechanism for rapid virus spread
Department of Virology, Faculty of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
Science 327:873-6. 2010
..Additional spreading mechanisms may exist for other viruses that also spread faster than predicted by replication kinetics...
- A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry
Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
Cell 99:13-22. 1999
- Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses
Craig R Johnson
Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA
J Gen Virol 92:1107-16. 2011
..Its discovery also provides a new potential target for immunological and pharmacological intervention in PRRS...
- Glycoprotein organization of Chikungunya virus particles revealed by X-ray crystallography
James E Voss
Institut Pasteur, Departement de Virologie, Unité de Virologie Structurale, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
Nature 468:709-12. 2010
..3), this work also provides new insight into the acid-triggered conformational change on the virus particle and its inbuilt inhibition mechanism in the immature complex...
- Dengue virus neutralization by human immune sera: role of envelope protein domain III-reactive antibody
W M P B Wahala
Department of Microbiology and Immunology, CB 7290 University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
Virology 392:103-13. 2009
..Our results have implications for understanding protective immunity following natural DENV infection and for evaluating DENV vaccines...
- West Nile virus recombinant DNA vaccine protects mouse and horse from virus challenge and expresses in vitro a noninfectious recombinant antigen that can be used in enzyme-linked immunosorbent assays
B S Davis
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522, USA
J Virol 75:4040-7. 2001
..This recombinant antigen has great potential to become the antigen of choice and will facilitate the standardization of reagents and implementation of WN virus surveillance in the United States and elsewhere...
- Crystal structure of glycoprotein B from herpes simplex virus 1
Ekaterina E Heldwein
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
Science 313:217-20. 2006
..The domain connectivity within a gB monomer would permit such a rearrangement, including long-range translocations linked to viral and cellular membranes...
- Structural basis of West Nile virus neutralization by a therapeutic antibody
Grant E Nybakken
Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Nature 437:764-9. 2005
..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
- Topology of the C-terminal tail of HIV-1 gp41: differential exposure of the Kennedy epitope on cell and viral membranes
Jonathan D Steckbeck
Center for Vaccine Research, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
PLoS ONE 5:e15261. 2010
..These data indicate apparently distinct CTT topologies for virion- and cell-associated Env species and add to the case for a reconsideration of CTT topology that is more complex than currently envisioned...
- Structure of hepatitis C virus envelope glycoprotein E2 antigenic site 412 to 423 in complex with antibody AP33
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, USA
J Virol 86:13085-8. 2012
..These structures establish two different modes of binding to E2 that antibodies adopt to neutralize diverse HCV...
- Analysis of epitopes on dengue virus envelope protein recognized by monoclonal antibodies and polyclonal human sera by a high throughput assay
Hong En Lin
Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
PLoS Negl Trop Dis 6:e1447. 2012
- Vaccine prevention of maternal cytomegalovirus infection
Robert F Pass
Department of Pediatrics, University of Alabama at Birmingham, Birmingham 35233, USA
N Engl J Med 360:1191-9. 2009
..Congenital infection with cytomegalovirus (CMV) is an important cause of hearing, cognitive, and motor impairments in newborns...
- Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure
Tommi A White
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
PLoS Pathog 6:e1001249. 2010
- Human broadly neutralizing antibodies to the envelope glycoprotein complex of hepatitis C virus
Department of Immunology and Microbial Science and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 109:6205-10. 2012
..The mAb panel will be useful for the design and development of vaccine candidates to elicit broadly neutralizing antibodies to HCV...
- Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses
Sheli R Radoshitzky
Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, Southborough, Massachusetts 01772, USA
Nature 446:92-6. 2007
..These data indicate that TfR1 is a cellular receptor for New World haemorrhagic fever arenaviruses...
- Naturally occurring antibodies that recognize linear epitopes in the amino terminus of the hepatitis C virus E2 protein confer noninterfering, additive neutralization
Alexander W Tarr
School of Molecular Medical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham, United Kingdom
J Virol 86:2739-49. 2012
- Biochemical and morphological properties of hepatitis C virus particles and determination of their lipidome
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
J Biol Chem 286:3018-32. 2011
..Taken together, we describe a rapid and efficient system for the production of large quantities of affinity-purified HCV allowing a comprehensive analysis of the infectious virion, including the determination of its lipid composition...
- CD81 is required for hepatitis C virus glycoprotein-mediated viral infection
Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, New York 10021, USA
J Virol 78:1448-55. 2004
..These data suggest a functional role for CD81 as a coreceptor for HCV glycoprotein-dependent viral cell entry...
- Role of N-linked glycans in the functions of hepatitis C virus envelope proteins incorporated into infectious virions
Institut Pasteur de Lille, Bâtiment IBL, 59021 Lille Cedex, France
J Virol 84:11905-15. 2010
..Furthermore, these carbohydrates form a "glycan shield" at the surface of the virion, which contributes to the evasion of HCV from the humoral immune response...
- Hepatitis C virus continuously escapes from neutralizing antibody and T-cell responses during chronic infection in vivo
Thomas von Hahn
Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10021, USA
Gastroenterology 132:667-78. 2007
- Ebola virus glycoprotein: proteolytic processing, acylation, cell tropism, and detection of neutralizing antibodies
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
J Virol 75:1576-80. 2001
..This finding provides evidence of shared neutralizing epitopes on GP and SGP molecules and indicates the potential of SGP to serve as a decoy for neutralizing antibodies...
- The disulfide bonds in glycoprotein E2 of hepatitis C virus reveal the tertiary organization of the molecule
Institut Pasteur, CNRS URA3015, Unité de Virologie Structurale, Paris, France
PLoS Pathog 6:e1000762. 2010
- Herpesvirus entry: an update
Patricia G Spear
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 77:10179-85. 2003
- Structural changes of envelope proteins during alphavirus fusion
Department of Biological Sciences, Purdue University, 915 W State Street, West Lafayette, Indiana 47907 2054, USA
Nature 468:705-8. 2010
..The amino- and carboxy-terminal domains of E2 each form immunoglobulin-like folds, consistent with the receptor attachment properties of E2...
- N-linked glycosylation of west nile virus envelope proteins influences particle assembly and infectivity
Sheri L Hanna
Department of Microbiology, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
J Virol 79:13262-74. 2005
..This suggests a multifaceted role for envelope N-linked glycosylation in WNV biology and tropism...
- Identification of a broadly cross-reacting and neutralizing human monoclonal antibody directed against the hepatitis C virus E2 protein
Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, DIBIT2, Via Olgettina 60, 20132 Milano, Italia
J Virol 82:1047-52. 2008
..Fab e137 was also able to inhibit cell culture-grown HCV (genotype 2a). These data indicate that broadly cross-reacting and cross-neutralizing antibodies are generated during HCV infection...
- Phylogenetic analysis of North American West Nile virus isolates, 2001-2004: evidence for the emergence of a dominant genotype
C Todd Davis
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, and Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77550 0609, USA
Virology 342:252-65. 2005
..The implications of these findings are discussed as they relate to transmission and spread of the virus in the Western Hemisphere...
- Structural analysis of a dengue cross-reactive antibody complexed with envelope domain III reveals the molecular basis of cross-reactivity
Claire M Midgley
Department of Medicine, Hammersmith Hospital Campus, Imperial College London, London W12 0NN, United Kingdom
J Immunol 188:4971-9. 2012
..Surprisingly, 2H12 facilitated little or no enhancement of infection. These data provide a structural basis for understanding Ab neutralization and enhancement of infection, which is crucial for the development of future dengue vaccines...
- Intrauterine transmission of cytomegalovirus to infants of women with preconceptional immunity
S B Boppana
Department of Pediatrics, University of Alabama at Birmingham, 35233, USA
N Engl J Med 344:1366-71. 2001
..Whether congenital CMV infection in the offspring of women who are seropositive for CMV can occur after maternal reinfection with a different strain of CMV is unknown...
- Toward a hepatitis C virus vaccine: the structural basis of hepatitis C virus neutralization by AP33, a broadly neutralizing antibody
Jane A Potter
Biomedical Sciences Research Complex, University of St Andrews, St Andrews, Fife, United Kingdom
J Virol 86:12923-32. 2012
..The structural details of this neutralizing epitope provide a starting point for the design of an immunogen capable of eliciting AP33-like antibodies...
- West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection
Carl W Davis
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
J Virol 80:1290-301. 2006
..We believe this is the first report of a pathogen interacting more efficiently with DC-SIGNR than with DC-SIGN. Our results should lead to the discovery of new mechanisms by which these well-studied lectins discriminate among ligands...
- Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia
J Gen Virol 93:72-82. 2012
..These studies have also demonstrated differential binding between mosquito and human cells...
- Structure of the dengue virus envelope protein after membrane fusion
Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 427:313-9. 2004
..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry...
- Mechanism of dengue virus broad cross-neutralization by a monoclonal antibody
Joseph J B Cockburn
Unité de Virologie Structurale, Departement de Virologie, Institut Pasteur, 75724 Paris Cedex 15, France
Structure 20:303-14. 2012
..The structures also highlight the mechanism by which A-strand Mabs disrupt the architecture of the mature virion, inducing premature fusion loop exposure and concomitant particle inactivation...
- Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia
Thi Ty Hang Vu
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam
PLoS Negl Trop Dis 4:e757. 2010
..These results provide a mechanistic basis to a marked change in the genetic structure of DENV-2 and more broadly underscore that an understanding of DENV evolutionary dynamics can inform the development of vaccines and anti-viral drugs...
- Molecular mechanisms of flavivirus membrane fusion
Institute of Virology, Medical University of Vienna, AT, Austria
Amino Acids 41:1159-63. 2011
..This includes the identification of a conserved histidine as being part of the pH sensor in the fusion protein that responds to the acidic pH and thus initiates the structural transitions driving fusion...
- Conformational changes of the flavivirus E glycoprotein
Department of Biological Sciences, Lilly Hall, 915 West State Street, Purdue University, West Lafayette, IN 47907, USA
Structure 12:1607-18. 2004
..Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses...
- The flavivirus precursor membrane-envelope protein complex: structure and maturation
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Science 319:1830-4. 2008
..The structure provides a basis for identifying the stages of its pH-directed conformational metamorphosis during maturation, ending with release of pr when budding from the host...
- Neutralizing antibody response to hepatitis C virus
Department of Pathology, School of Medicine, Stanford University, Stanford, CA 94305, USA
Viruses 3:2127-45. 2011
- Structure and function analysis of therapeutic monoclonal antibodies against dengue virus type 2
Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
J Virol 84:9227-39. 2010
..Overall, these studies define the structural and functional complexity of antibodies against DENV-2 with protective potential...
- Herpes simplex virus glycoproteins gH/gL and gB bind Toll-like receptor 2, and soluble gH/gL is sufficient to activate NF-κB
Department of Experimental Pathology, Alma Mater Studiorum University of Bologna, Bologna, Italy
J Virol 86:6555-62. 2012
..Thus, whereas both HSV gH/gL and gB are ligands to TLR2, only gH/gL is sufficient to initiate a signaling cascade which leads to NF-κB activation...
- The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus
Catherine L Hunt
Dept Microbiology, University of Iowa, Iowa City, IA 52242, USA
J Virol 85:334-47. 2011
..Our findings demonstrate for the first time that Axl enhances macropinocytosis, thereby increasing productive ZEBOV entry...
- Chikungunya virus emergence is constrained in Asia by lineage-specific adaptive landscapes
Konstantin A Tsetsarkin
Institute for Human Infections and Immunity, Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA
Proc Natl Acad Sci U S A 108:7872-7. 2011
..albopictus-adapted African strains. These results underscore how different adaptive landscapes occupied by closely related viral genotypes can profoundly affect the outcome of viral evolution and disease emergence...
- Characterization of the envelope glycoproteins associated with infectious hepatitis C virus
Institut Pasteur de Lille, Center for Infection and Immunity of Lille, F 59019 Lille, France
J Virol 84:10159-68. 2010
..Overall, our study fills a gap in the description of HCV outer morphology and should guide further investigations into virus entry and assembly...
- Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes
Wayne D Crill
Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, P O Box 2087, Fort Collins, CO 80522, USA
J Virol 78:13975-86. 2004
..The third epitope consists of discontinuous residues that are structurally related to the strictly conserved tryptophan at dengue virus serotype 2 E-glycoprotein position 231...
- Ebolavirus is internalized into host cells via macropinocytosis in a viral glycoprotein-dependent manner
Influenza Research Institute, Department of Pathological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
PLoS Pathog 6:e1001121. 2010
..These findings provide new insights into the lifecycle of Ebolavirus and may aid in the development of therapeutics for Ebolavirus infection...
- The major determinant of attenuation in mice of the Candid1 vaccine for Argentine hemorrhagic fever is located in the G2 glycoprotein transmembrane domain
Cesar G Albarino
Centers for Disease Control and Prevention, MS G14, Atlanta, GA 30329, USA
J Virol 85:10404-8. 2011
..This work provides insight into the molecular mechanisms of attenuation of the only arenavirus vaccine currently available...
- Development of a recombinant tetravalent dengue virus vaccine: immunogenicity and efficacy studies in mice and monkeys
David E Clements
Hawaii Biotech, Inc, 99 193 Aiea Heights Drive, Aiea, HI 96701, United States
Vaccine 28:2705-15. 2010
..These results support the development of a dengue virus tetravalent vaccine based on the four 80E subunits produced in the Drosophila S2 cell expression system...
- The development of therapeutic antibodies that neutralize homologous and heterologous genotypes of dengue virus type 1
Department of Medicine, Washington University School of Medicine, St Louis, Missouri, United States of America
PLoS Pathog 6:e1000823. 2010
..Overall, our experiments define a complex structural epitope on DIII of DENV-1 that can be recognized by protective antibodies with therapeutic potential...
- Human endogenous retrovirus rec interferes with germ cell development in mice and may cause carcinoma in situ, the predecessor lesion of germ cell tumors
Uwe M Galli
Department of Virology, Bldg 47, University of Saarland Medical School, 66421 Homburg Saar, Germany
Oncogene 24:3223-8. 2005
..This provides the first direct evidence that the expression of a human endogenous retroviral gene previously established as a marker in human germ cell tumors may contribute to organ-specific tumorigenesis in a transgenic mouse model...
- Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells
W D Crill
Arbovirus Disease Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
J Virol 75:7769-73. 2001
..These results extend our understanding of the structure-function relationships in the E glycoprotein of DEN virus and provide the first direct evidence that domain III encodes the primary flavivirus receptor-binding motif...
- Maturation of West Nile virus modulates sensitivity to antibody-mediated neutralization
Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS Pathog 4:e1000060. 2008
..Thus, in addition to a role in facilitating viral entry, changes in E protein arrangement associated with maturation modulate neutralization sensitivity and introduce an additional layer of complexity into humoral immunity against WNV...
- Identification of multiple protective epitopes (protectopes) in the central conserved domain of a prototype human respiratory syncytial virus G protein
Centre d Immunologie Pierre Fabre, 74164 Saint Julien en Genevois cedex, France
J Virol 73:5637-45. 1999
..Should these murine protectopes also be implicated in human LRT protection, their clustering around the highly conserved cysteine noose region will have important implications for the development of RSV vaccines...
- Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor
R J Geraghty
Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
Science 280:1618-20. 1998
..HveC was expressed in human cells of epithelial and neuronal origin; it is the prime candidate for the coreceptor that allows both HSV-1 and HSV-2 to infect epithelial cells on mucosal surfaces and spread to cells of the nervous system...
- Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
J M MacKenzie
Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
J Virol 75:10787-99. 2001
..The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent maturation occurs via the secretory pathway...
- Molecular epidemiological analysis of Japanese encephalitis virus in China
Huan Yu Wang
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 YingXinJie, XuanWuQu, Beijing 100052, People s Republic of China
J Gen Virol 88:885-94. 2007
..The results suggest that genotype 1 JEV was introduced to China around 1979 and that JEV strains belonging to genotypes 1 and 3 circulate in China...
- Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography
Abteilung für Molekulare Strukturbiologie, Max Planck Institut fur Biochemie, Am Klopferspitz 18, D 82152 Martinsried, Germany
Proc Natl Acad Sci U S A 102:4729-34. 2005
..This study thus provides 3D structural information regarding the prefusion conformation of an intact unstained retrovirus surface protein...
- Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL
Tirumala K Chowdary
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA
Nat Struct Mol Biol 17:882-8. 2010
..Formation of a gB-gH-gL complex is critical for fusion and is inhibited by a neutralizing antibody, making the gB-gH-gL interface a promising antiviral target...
- Serological response to human endogenous retrovirus K in melanoma patients correlates with survival probability
Retroelement Section, Paul Ehrlich Institut, Langen, Germany
AIDS Res Hum Retroviruses 24:717-23. 2008
..027) in melanoma patients with the early stages of the disease (AJCC I-III). This is the first report that the humoral anti-HERV-K immune response may provide additional prognostic information to that of established melanoma markers...
- Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism
Department of Molecular Biology, Princeton University, NJ 08544, USA
Proc Natl Acad Sci U S A 102:18153-8. 2005
- Natural history of human respiratory syncytial virus inferred from phylogenetic analysis of the attachment (G) glycoprotein with a 60-nucleotide duplication
Unidad de Biología Viral, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain
J Virol 80:975-84. 2006
..We have taken advantage of this situation to reexamine the molecular epidemiology of HRSV and to explore the natural history of this important human pathogen...
- Phylogeography and evolutionary history of dengue virus type 3
Josélio M G Araújo
Laboratory of Flavivirus, Oswaldo Cruz Institute FIOCRUZ, Rio de Janeiro, Brazil
Infect Genet Evol 9:716-25. 2009
- Depletion of interfering antibodies in chronic hepatitis C patients and vaccinated chimpanzees reveals broad cross-genotype neutralizing activity
Division of Hematology and Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:7537-41. 2009
..Our studies contribute to understanding the mechanisms of antibody-mediated neutralization and interference and provide a practical approach to the development of more potent and broadly reactive hepatitis C immune globulins...
- Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited
CNRS, UMR2472, INRA, UMR1157, IFR 115, Virologie Moléculaire et Structurale, Gif sur Yvette, France
Cell Mol Life Sci 65:1716-28. 2008
..Besides the similarities with other viral fusion machineries, the fusion properties and structures of G also reveal some striking particularities that invite us to reconsider a few dogmas concerning fusion proteins...
- Entomologic and virologic investigation of Chikungunya, Singapore
Lee Ching Ng
National Environment Agency, Singapore
Emerg Infect Dis 15:1243-9. 2009
..These findings led to a revision of the current vector control strategy in Singapore. This report highlights the use of entomologic and virologic data to assist in the control of chikungunya in disease-endemic areas...
- The envelope glycoprotein domain III of dengue virus serotypes 1 and 2 inhibit virus entry
J F L Chin
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore
Microbes Infect 9:1-6. 2007
..This observation may be of importance when formulating a generic vaccine that is effective against all dengue virus serotypes...
- Isolation and characterization of human monoclonal antibodies from individuals infected with West Nile Virus
Crucell Holland B V, P O Box 2048, 2301 CA, Leiden, The Netherlands
J Virol 80:6982-92. 2006
- Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles
Aaron Diamond AIDS Research Center and Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 100:7271-6. 2003
..However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex...
- Dendrimer functionalization with a membrane-interacting domain of herpes simplex virus type 1: towards intracellular delivery
Tom P Carberry
Molecular Design Institute, Department of Chemistry, New York University, NY 10003, USA
Chemistry 18:13678-85. 2012
..Our results suggest that the presented peptidodendrimeric scaffold may be a promising material for efficient drug delivery...
- NKp44 receptor mediates interaction of the envelope glycoproteins from the West Nile and dengue viruses with NK cells
Shraga Segal Department of Microbiology and Immunology and National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
J Immunol 183:2610-21. 2009
..Our findings show that triggering of NK cells by flavivirus is mediated by interaction of NKp44 with the flavivirus envelope protein...
- Physical and functional interactions of human endogenous retrovirus proteins Np9 and rec with the promyelocytic leukemia zinc finger protein
Institute of Virology, University of Saarland Medical School, Homburg Saar D 66421, Germany
J Virol 81:5607-16. 2007
..Thus, the human endogenous retrovirus proteins Np9 and Rec may act oncogenically by derepressing c-myc through the inhibition of PLZF...
- Cellular binding of hepatitis C virus envelope glycoprotein E2 requires cell surface heparan sulfate
Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
J Biol Chem 278:41003-12. 2003
..Docking of E2 to cellular HSPG may be the initial step in the interaction between HCV and the cell surface resulting in receptor-mediated entry and initiation of infection...
- Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment
Helen L Zenner
Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
J Virol 85:8012-21. 2011
- A small-molecule dengue virus entry inhibitor
Qing Yin Wang
Novartis Institute for Tropical Diseases, 10 Biopolis Rd, Chromos Building, Singapore 138670, Singapore
Antimicrob Agents Chemother 53:1823-31. 2009
..It arrests dengue virus in vesicles that colocalize with endocytosed dextran and inhibits NS3 expression. The inhibitors described in this report can serve as molecular probes for the study of the entry of flavivirus into host cells...
- Mutational evidence of internal fusion loops in herpes simplex virus glycoprotein B
Brian P Hannah
Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 240 S 40th Street, Levy Building R233, Philadelphia, PA 19104, USA
J Virol 81:4858-65. 2007
..We hypothesize that two loops in domain 1 of HSV gB function as fusion loops. Our data are further evidence that gB is a viral fusogen and suggest clues as to how gB may function...
- Evidence that TMPRSS2 activates the severe acute respiratory syndrome coronavirus spike protein for membrane fusion and reduces viral control by the humoral immune response
Institute of Virology, Hannover Medical School, 30625 Hannover, Germany
J Virol 85:4122-34. 2011
..In summary, we show that TMPRSS2 might promote viral spread and pathogenesis by diminishing viral recognition by neutralizing antibodies and by activating SARS S for cell-cell and virus-cell fusion...
- Early events in Kaposi's sarcoma-associated herpesvirus infection of target cells
H M Bligh Cancer Research Laboratories, Dept of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064, USA
J Virol 84:2188-99. 2010
..The various signaling events triggered by infection, and their potential roles in the different stages of infection and disease pathogenesis, are summarized...
- The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates
Division of Human Retrovirology Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Cell 85:1135-48. 1996
..The ability of various members of the chemokine receptor family to support the early stages of HIV-1 infection helps to explain viral tropism and beta-chemokine inhibition of primary HIV-1 isolates...
- Ebola virus glycoprotein needs an additional trigger, beyond proteolytic priming for membrane fusion
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA
PLoS Negl Trop Dis 5:e1395. 2011
..After entry into host endosomes, GP(1,2) is enzymatically cleaved by endosomal cathepsins B and L, a necessary step in infection. However, the functional effects of the cleavage on the glycoprotein are unknown...
- Phylogeography and population dynamics of dengue viruses in the Americas
Orchid M Allicock
Department of Preclinical Sciences, Faculty of Medical Sciences, University of the West Indies, St Augustine, Trinidad and Tobago
Mol Biol Evol 29:1533-43. 2012
..However, for DENV-3 and DENV-4, the more stochastic "equal rates" model was preferred...
- Antibodies to envelope glycoprotein of dengue virus during the natural course of infection are predominantly cross-reactive and recognize epitopes containing highly conserved residues at the fusion loop of domain II
Chih Yun Lai
Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
J Virol 82:6631-43. 2008
..These findings have implications for our understanding of the pathogenesis of dengue and for the future design of subunit vaccine against DENV as well...