capsid proteins

Summary

Summary: Proteins that form the CAPSID of VIRUSES.

Top Publications

  1. ncbi Norovirus classification and proposed strain nomenclature
    Du Ping Zheng
    Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Virology 346:312-23. 2006
  2. pmc Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998
    B A Brown
    Division of Viral Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Atlanta, Georgia 30333, USA
    J Virol 73:9969-75. 1999
  3. ncbi Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
    Diane M Roberts
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 441:239-43. 2006
  4. ncbi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
  5. ncbi Prevalence of polyomavirus BK and JC infection and replication in 400 healthy blood donors
    Adrian Egli
    Transplantation Virology, Institute for Medical Microbiology, Department of Biomedicine, University of Basel, Switzerland
    J Infect Dis 199:837-46. 2009
  6. pmc X-ray structures of the hexameric building block of the HIV capsid
    Owen Pornillos
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 137:1282-92. 2009
  7. pmc HIV capsid is a tractable target for small molecule therapeutic intervention
    Wade S Blair
    Pfizer Global Research and Development, La Jolla Laboratories, San Diego, California, United States of America
    PLoS Pathog 6:e1001220. 2010
  8. pmc Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens
    W Allan Nix
    Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 44:2698-704. 2006
  9. pmc Efficient intracellular assembly of papillomaviral vectors
    Christopher B Buck
    Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892 4263, USA
    J Virol 78:751-7. 2004
  10. pmc Evolutionary genetics of human enterovirus 71: origin, population dynamics, natural selection, and seasonal periodicity of the VP1 gene
    Kok Keng Tee
    Laboratory of Molecular Virology and Epidemiology, AIDS Research Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Virol 84:3339-50. 2010

Detail Information

Publications391 found, 100 shown here

  1. ncbi Norovirus classification and proposed strain nomenclature
    Du Ping Zheng
    Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Virology 346:312-23. 2006
    ..Of these, five clusters in G2 and one in G1 are newly described. This analysis can serve as the basis for a standardized nomenclature to genetically describe NoV strains...
  2. pmc Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998
    B A Brown
    Division of Viral Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Atlanta, Georgia 30333, USA
    J Virol 73:9969-75. 1999
    ..Its worldwide circulation and potential to cause severe disease underscore the need for additional surveillance and improved methods to identify EV71 in human disease...
  3. ncbi Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
    Diane M Roberts
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 441:239-43. 2006
    ..to circumvent pre-existing anti-vector immunity by removing key neutralizing epitopes on the surface of viral capsid proteins. Such chimaeric viral vectors may have important practical implications for vaccination and gene therapy.
  4. ncbi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
    ..Thus, VP2-28 could be used as the surrogate biomarker in the potency testing of candidate EV71 vaccines...
  5. ncbi Prevalence of polyomavirus BK and JC infection and replication in 400 healthy blood donors
    Adrian Egli
    Transplantation Virology, Institute for Medical Microbiology, Department of Biomedicine, University of Basel, Switzerland
    J Infect Dis 199:837-46. 2009
    ....
  6. pmc X-ray structures of the hexameric building block of the HIV capsid
    Owen Pornillos
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 137:1282-92. 2009
    ..The structures also clarify the molecular basis for capsid assembly inhibition and should facilitate structure-based drug design strategies...
  7. pmc HIV capsid is a tractable target for small molecule therapeutic intervention
    Wade S Blair
    Pfizer Global Research and Development, La Jolla Laboratories, San Diego, California, United States of America
    PLoS Pathog 6:e1001220. 2010
    ..Our data demonstrate that broad-spectrum antiviral activity can be achieved by targeting this new binding site and reveal HIV CA as a tractable drug target for HIV therapy...
  8. pmc Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens
    W Allan Nix
    Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 44:2698-704. 2006
    ....
  9. pmc Efficient intracellular assembly of papillomaviral vectors
    Christopher B Buck
    Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892 4263, USA
    J Virol 78:751-7. 2004
    ..The results suggest that the intracellular assembly of papillomavirus structural proteins around heterologous reporter plasmids is surprisingly promiscuous and may be driven primarily by a size discrimination mechanism...
  10. pmc Evolutionary genetics of human enterovirus 71: origin, population dynamics, natural selection, and seasonal periodicity of the VP1 gene
    Kok Keng Tee
    Laboratory of Molecular Virology and Epidemiology, AIDS Research Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Virol 84:3339-50. 2010
    ..We discuss the consequences of the evolutionary dynamics of EV-71 for vaccine design and compare its phylodynamic behavior with that of influenza virus...
  11. pmc Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
    ..In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity...
  12. pmc Small-molecule inhibition of human immunodeficiency virus type 1 infection by virus capsid destabilization
    Jiong Shi
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    J Virol 85:542-9. 2011
    ..This study highlights uncoating as a step in the HIV-1 life cycle that is susceptible to small molecule intervention...
  13. ncbi Prevalence of serum IgG and neutralizing factors against adeno-associated virus (AAV) types 1, 2, 5, 6, 8, and 9 in the healthy population: implications for gene therapy using AAV vectors
    Sylvie Boutin
    Laboratoire d Immunologie, Genethon R and D, Evry Cedex, France
    Hum Gene Ther 21:704-12. 2010
    ..Better characterization of the preexisting humoral responses to the AAV capsid and cross-reactivity will allow development of new strategies to circumvent AAV acquired immune responses...
  14. ncbi X-ray crystallographic structure of the Norwalk virus capsid
    B V Prasad
    Verna and Marrs McLean Department of Biochemistry, Division of Molecular Virology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 286:287-90. 1999
    ..This subdomain, located at the exterior of the capsid, has the largest sequence variation among Norwalk-like human caliciviruses and is likely to contain the determinants of strain specificity and cell binding...
  15. pmc Novel surveillance network for norovirus gastroenteritis outbreaks, United States
    Everardo Vega
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 17:1389-95. 2011
    ..4 New Orleans, which first emerged in October 2009. Analysis of the complete capsid and P2 region sequences confirmed that GII.4 New Orleans is distinct from previous GII.4 variants, including GII.4 Minerva (2006b)...
  16. pmc Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks
    Hongrong Liu
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA
    Science 329:1038-43. 2010
    ..6 angstroms resolution by cryo-electron microscopy (cryo-EM), revealing in situ atomic models of three minor capsid proteins (IIIa, VIII, and IX), extensions of the (penton base and hexon) major capsid proteins, and interactions ..
  17. ncbi Piscine reovirus encodes a cytotoxic, non-fusogenic, integral membrane protein and previously unrecognized virion outer-capsid proteins
    Tim Key
    Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H4R2, Canada
    J Gen Virol 94:1039-50. 2013
    ..The presence of a novel integral membrane protein and two previously unrecognized, essential outer-capsid proteins has important implications for the biology, evolution and taxonomic classification of this virus.
  18. pmc Evidence for direct involvement of the capsid protein in HIV infection of nondividing cells
    Masahiro Yamashita
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    PLoS Pathog 3:1502-10. 2007
    ..Our data suggest that CA is directly involved at some step in the viral life cycle that is important for infection of nondividing cells...
  19. pmc Atomic-level modelling of the HIV capsid
    Owen Pornillos
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nature 469:424-7. 2011
    ..Rigid-body rotations around two assembly interfaces appear sufficient to generate the full range of continuously varying lattice curvature in the fullerene cone...
  20. ncbi Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides
    Damian Guang Wei Foo
    Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    Virus Res 125:61-8. 2007
    ..Altogether, these data indicate that SP70 represents a promising candidate for an effective synthetic peptide-based vaccine against EV71...
  21. pmc Target cell type-dependent modulation of human immunodeficiency virus type 1 capsid disassembly by cyclophilin A
    Yuan Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 83:10951-62. 2009
    ..In both cell types examined, decreased stability of the capsid was associated with a decrease in the efficiency of HIV-1 infection...
  22. pmc Molecular epidemiology of genogroup II-genotype 4 noroviruses in the United States between 1994 and 2006
    Du Ping Zheng
    Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 48:168-77. 2010
    ..We propose that GII.4 subclusters be defined as having >5% sequence variation between strains. Our data confirm other studies on the rapid emergence and displacement of highly virulent GII.4 strains...
  23. pmc Crystal structure of human adenovirus at 3.5 A resolution
    Vijay S Reddy
    The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 329:1071-5. 2010
    ..The high-resolution structure provides a substantial advance toward understanding the assembly and cell entry mechanisms of a large double-stranded DNA virus and provides new opportunities for improving adenovirus-mediated gene transfer...
  24. pmc 3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry
    Xing Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 7364, USA
    Cell 141:472-82. 2010
    ..These results suggest a well-orchestrated process of nonenveloped virus entry involving autocleavage of the penetration protein prior to exposure of its membrane-insertion finger...
  25. pmc Cyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells
    Theodora Hatziioannou
    Aaron Diamond AIDS Research Center and Rockefeller University, New York, New York 10021, USA
    J Virol 79:176-83. 2005
    ..We speculate that CypA and/or CypA-related proteins affect the fate of incoming HIV-1 capsid either directly or by modulating interactions with unidentified host cell factors...
  26. ncbi Genogroup-specific PCR primers for detection of Norwalk-like viruses
    Shigeyuki Kojima
    Section of Infectious Disease, R and D Center, BML, 1361 1, Matoba, Kawagoe, Saitama 350 1101, Japan
    J Virol Methods 100:107-14. 2002
    ..Therefore, new primer sets are extremely useful for the amplification and rapid diagnosis of nonbacterial gastroenteritis due to NLV as well as for epidemiological studies...
  27. pmc Comparative genomics of foot-and-mouth disease virus
    C Carrillo
    Department of Pathobiology and Veterinary Science, University of Connecticut, 61 N Eagleville Road, Unit 3089, Storrs, CT 06269 3089, USA
    J Virol 79:6487-504. 2005
    ..Insights into viral RNA sequence conservation and variability and genetic diversity in nature will likely impact our understanding of FMDV infections, host range, and transmission...
  28. pmc Functional surfaces of the human immunodeficiency virus type 1 capsid protein
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City 84132, USA
    J Virol 77:5439-50. 2003
    ..Finally, the identification of replication-defective mutants with normal viral assembly phenotypes indicates that CA also performs important nonstructural functions at early stages of the viral life cycle...
  29. pmc Annexin II binds to capsid protein VP1 of enterovirus 71 and enhances viral infectivity
    Su Lin Yang
    Graduate Institute of Life Sciences, Taiwan Centers for Disease Control, Taipei, Taiwan
    J Virol 85:11809-20. 2011
    ..Our data suggest that binding of EV71 to Anx2 on the cell surface can enhance viral entry and infectivity, especially at a low infective dose...
  30. pmc Rotavirus anti-VP6 secretory immunoglobulin A contributes to protection via intracellular neutralization but not via immune exclusion
    Blaise Corthesy
    R and D Laboratory of the Division of Immunology and Allergy, DMI CHUV, Rue du Bugnon, 1011 Lausanne, Switzerland
    J Virol 80:10692-9. 2006
    ..These findings identify a mechanism whereby the well-conserved immunodominant VP6 protein can function as a target for heterotypic antibodies and protective immunity...
  31. pmc A viral assembly factor promotes AAV2 capsid formation in the nucleolus
    Florian Sonntag
    Department of Tumour Virology, German Cancer Research Center, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 107:10220-5. 2010
    ..AAP targets newly synthesized capsid proteins to this organelle and in addition fulfils a function in the assembly reaction itself...
  32. pmc Assembly properties of the human immunodeficiency virus type 1 CA protein
    Barbie K Ganser-Pornillos
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132, USA
    J Virol 78:2545-52. 2004
    ..In further support of this model, we demonstrate that the considerable morphological variation seen for conical HIV-1 capsids can be recapitulated in idealized capsid models by altering the distribution of pentamers...
  33. pmc Plus- and minus-end directed microtubule motors bind simultaneously to herpes simplex virus capsids using different inner tegument structures
    Kerstin Radtke
    Institute of Virology, Hannover Medical School, Hannover, Germany
    PLoS Pathog 6:e1000991. 2010
    ..Thus, these associated motors either engage in a tug-of-war or their activities are coordinately regulated to achieve net transport either to the nucleus during cell entry or to cytoplasmic membranes for envelopment during assembly...
  34. pmc Display of VP1 on the surface of baculovirus and its immunogenicity against heterologous human enterovirus 71 strains in mice
    Tao Meng
    Animal Health Biotechnology, Temasek Life Sciences Laboratory, Republic of Singapore
    PLoS ONE 6:e21757. 2011
    ..VP1 is one of the major immunogenic capsid protein of EV71 and plays a crucial role in viral infection. Antibodies against VP1 are important for virus neutralization...
  35. pmc Sequencing and analysis of JC virus DNA from natalizumab-treated PML patients
    Carl E Reid
    Biogen Idec Inc, Cambridge, MA 02142, USA
    J Infect Dis 204:237-44. 2011
    ..JCV sequence variation and rearrangements influence viral pathogenicity and tropism. To better understand PML development, we analyzed viral DNA sequences in blood, CSF and/or urine of natalizumab-treated PML patients...
  36. pmc Typing of human enteroviruses by partial sequencing of VP1
    M S Oberste
    Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 37:1288-93. 1999
    ..The technique may also be useful for the rapid determination of whether viruses isolated during an outbreak are epidemiologically related...
  37. ncbi Outbreak of poliomyelitis in Hispaniola associated with circulating type 1 vaccine-derived poliovirus
    Olen Kew
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention CDC, 1600 Clifton Road, Atlanta, GA 30333, USA
    Science 296:356-9. 2002
    ..The vaccine-derived poliovirus associated with the outbreak had biological properties indistinguishable from those of wild poliovirus...
  38. pmc Circulation of endemic type 2 vaccine-derived poliovirus in Egypt from 1983 to 1993
    Chen Fu Yang
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 77:8366-77. 2003
    ..The potential for cVDPVs to circulate in populations with low immunity to poliovirus has important implications for current and future strategies to eradicate polio worldwide...
  39. pmc The VP1 unique region of parvovirus B19 and its constituent phospholipase A2-like activity
    Simone Dorsch
    Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg, Germany
    J Virol 76:2014-8. 2002
    ..By using subfragments, we demonstrated an association between the phospholipase A2-like activity and the carboxy-terminal domain of the VP1 unique region...
  40. pmc Epidemiology of enterovirus 71 in the Netherlands, 1963 to 2008
    Sabine van der Sanden
    National Institute for Public Health and the Environment, RIVM, P O Box 1, 3720 BA Bilthoven, The Netherlands
    J Clin Microbiol 47:2826-33. 2009
    ....
  41. ncbi Structure of a major immunogenic site on foot-and-mouth disease virus
    D Logan
    Laboratory of Molecular Biophysics, University of Oxford, UK
    Nature 362:566-8. 1993
    ..But here its structure has become sufficiently ordered to allow us to describe an unambiguous conformation, which we relate to some key biological properties of the virus...
  42. pmc Frequency and dynamics of recombination within different species of human enteroviruses
    Peter Simmonds
    Virus Evolution Group, Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, United Kingdom
    J Virol 80:483-93. 2006
    ....
  43. pmc Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays
    Yanis L Tolstov
    Molecular Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Int J Cancer 125:1250-6. 2009
    ..Our study demonstrates that MCV is a widespread but previously unrecognized human infection...
  44. ncbi Phylogenetic designation of enterovirus 71 genotypes and subgenotypes using complete genome sequences
    Yoke Fun Chan
    Department of Medical Microbiology, University Malaya, Kuala Lumpur, Malaysia
    Infect Genet Evol 10:404-12. 2010
    ..Hence, in the absence of full genome sequences, it is proposed here that a combination of VP1 and 3D RNA polymerase gene sequences be used for initial genotyping of EV-71 isolates...
  45. pmc Computational analysis identifies human adenovirus type 55 as a re-emergent acute respiratory disease pathogen
    Michael P Walsh
    Department of Bioinformatics and Computational Biology, George Mason University, 10900 University Blvd, MSN 5B3, Manassas, Virginia 20110, USA
    J Clin Microbiol 48:991-3. 2010
    ..4% HAdV-B14. Sporadic appearances as a re-emergent pathogen and misidentification as "HAdV-B11a" are due to this partial hexon...
  46. pmc Eclipse phase of herpes simplex virus type 1 infection: Efficient dynein-mediated capsid transport without the small capsid protein VP26
    Katinka Döhner
    Institut fur Virologie, OE5230, Medizinische Hochschule Hannover, Carl Neuberg Str 1, D 30623 Hannover, Germany
    J Virol 80:8211-24. 2006
    ..Thus, even in the absence of the potential viral dynein receptor VP26, HSV-1 used MT and dynein for efficient nuclear targeting. These data suggest that besides VP26, HSV-1 encodes other receptors for dynein or dynactin...
  47. pmc Transportin 3 promotes a nuclear maturation step required for efficient HIV-1 integration
    Lihong Zhou
    Wohl Virion Centre, Division of Infection and Immunity, University College London, London, United Kingdom
    PLoS Pathog 7:e1002194. 2011
    ..Furthermore Tnp3 binds the viral capsid proteins and tRNAs incorporated into viral particles...
  48. pmc Structural convergence between Cryo-EM and NMR reveals intersubunit interactions critical for HIV-1 capsid function
    In Ja L Byeon
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Cell 139:780-90. 2009
    ..In the tubular assembly, CA intermolecular interfaces vary slightly, accommodating the asymmetry present in tubes. This provides the necessary plasticity to allow for controlled virus capsid dis/assembly...
  49. pmc Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion
    M Mathieu
    , CNRS-UPR 9053 1, , 91198 Gif-sur-Yvette Cedex, France
    EMBO J 20:1485-97. 2001
    ..we present the crystal structure of VP6 determined to 2 A resolution and describe its interactions with other capsid proteins by fitting the atomic model into electron cryomicroscopic reconstructions of viral particles...
  50. pmc Progressive multifocal leukoencephalopathy (PML) development is associated with mutations in JC virus capsid protein VP1 that change its receptor specificity
    Leonid Gorelik
    Biogen Idec Inc, Cambridge, MA, USA
    J Infect Dis 204:103-14. 2011
    ..JCV VP1 substitutions are acquired intrapatient and might favor JCV brain invasion through abrogation of sialic acid binding with peripheral cells, while maintaining sialic acid-independent binding with brain cells...
  51. pmc Arrangement of L2 within the papillomavirus capsid
    Christopher B Buck
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 5624, USA
    J Virol 82:5190-7. 2008
    ..Papillomaviruses encode two capsid proteins, L1 and L2...
  52. ncbi Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein
    Vanda Bartonova
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:32024-33. 2008
    ..virions followed by proteolytic disassembly of the Gag protein shell and subsequent assembly of processed capsid proteins (CA) into the mature HIV-1 core...
  53. ncbi Degenerate RNA packaging signals in the genome of Satellite Tobacco Necrosis Virus: implications for the assembly of a T=1 capsid
    David H J Bunka
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    J Mol Biol 413:51-65. 2011
    ..These results are consistent with an assembly mechanism based on kinetically driven folding of the RNA...
  54. pmc Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes
    Zhijian Wu
    Gene Therapy Center, 7113 Thurston Building, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
    J Virol 80:11393-7. 2006
    ....
  55. pmc HPV-16 E2 contributes to induction of HPV-16 late gene expression by inhibiting early polyadenylation
    Cecilia Johansson
    Section of Medical Microbiology, Department of Laboratory Medicine, Lund University, Lund, Sweden
    EMBO J 31:3212-27. 2012
    ..We speculate that the accumulation of high levels of E2 during the viral life cycle, not only turns off the expression of the pro-mitotic viral E6 and E7 genes, but also induces the expression of the late HPV genes L1 and L2...
  56. pmc Atomic model of CPV reveals the mechanism used by this single-shelled virus to economically carry out functions conserved in multishelled reoviruses
    Xuekui Yu
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 7364, USA
    Structure 19:652-61. 2011
    ..TP is also simplified, containing only domains involved in RNA capping. Our results illustrate how CPV proteins have evolved in a coordinative manner to economically carry out their conserved functions...
  57. pmc Conservation of a packaging signal and the viral genome RNA packaging mechanism in alphavirus evolution
    Dal Young Kim
    Department of Microbiology, University of Alabama, Birmingham, AL 35294 2170, USA
    J Virol 85:8022-36. 2011
    ..except those belonging to the Semliki Forest virus (SFV) clade, have PSs which can be recognized by the capsid proteins of heterologous alphaviruses...
  58. pmc Structures of Merkel cell polyomavirus VP1 complexes define a sialic acid binding site required for infection
    Ursula Neu
    Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany
    PLoS Pathog 8:e1002738. 2012
    ..Since cell-surface glycans typically serve as primary attachment receptors for many viruses, we identify here a new role for glycans in mediating, and perhaps even modulating, post-attachment entry processes...
  59. ncbi Evaluation of a monoclonal antibody-based approach for the selection of foot-and-mouth disease (FMD) vaccine strains
    M Mahapatra
    Institute for Animal Health, Pirbright Laboratory, Ash Road, Woking, Surrey GU24 0NF, UK
    Vet Microbiol 126:40-50. 2008
    ....
  60. pmc An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles
    Jun Lin
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA
    J Virol 85:9974-83. 2011
    ..poliovirus (160S) converts to a cell-entry intermediate (135S) particle, resulting in the externalization of capsid proteins VP4 and the amino terminus of VP1 (residues 1 to 53)...
  61. ncbi Molecular epidemiology of chicken anemia virus in Nigeria
    M F Ducatez
    Institute of Immunology, Laboratoire National de Santé, Luxembourg, Grand Duchy of Luxembourg
    Arch Virol 151:97-111. 2006
    ..This first molecular epidemiological study of CAV in Africa shows that the Nigerian strains cluster with viruses from very diverse geographic origins and were almost as diverse (4.4%) as all other strains combined (5.8%)...
  62. ncbi Structure of full-length HIV-1 CA: a model for the mature capsid lattice
    Barbie K Ganser-Pornillos
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 131:70-9. 2007
    ..Interactions between the two domains of CA further stabilize the hexamer and provide a structural explanation for the mechanism of action of known HIV-1 assembly inhibitors...
  63. pmc In vivo mechanisms of vaccine-induced protection against HPV infection
    Patricia M Day
    Laboratory of Cellular Oncology, National Cancer Institute National Institutes of Health, Bethesda, MD 20892, USA
    Cell Host Microbe 8:260-70. 2010
    ..Regardless of the concentration, L2 vaccine-induced antibodies allow BM association but prevent association with the cell surface. Thus, we have revealed distinct mechanisms of vaccine-induced inhibition of virus infection in vivo...
  64. pmc The specificity of TRIM5 alpha-mediated restriction is influenced by its coiled-coil domain
    Pierre V Maillard
    Global Health Institute, School of Life Sciences, and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Virol 84:5790-801. 2010
    ..These results indicate that the PRYSPRY and coiled-coil domains cooperate to determine the specificity of TRIM5 alpha-mediated capture of retroviral capsids, shedding new light on this complex event...
  65. ncbi Mutations in VP2 and VP1 capsid proteins increase infectivity and mouse lethality of enterovirus 71 by virus binding and RNA accumulation enhancement
    Sheng Wen Huang
    The Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan
    Virology 422:132-43. 2012
    ..In sum, VP2(149M) and VP1(145E) mutations cooperatively promote viral binding and RNA accumulation of EV71, contributing to viral infectivity in vitro and mouse lethality in vivo...
  66. pmc Porcine noroviruses related to human noroviruses
    Qiu hong Wang
    Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA
    Emerg Infect Dis 11:1874-81. 2005
    ..These results raise concerns of whether subclinically infected adult swine may be reservoirs of new human NoVs or if porcine/human GII recombinants could emerge...
  67. pmc Quantitation of human seroresponsiveness to Merkel cell polyomavirus
    Diana V Pastrana
    Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland, USA
    PLoS Pathog 5:e1000578. 2009
    ..This suggests that a VLP-based vaccine could be effective for preventing the initial establishment of MCV infection...
  68. pmc A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1
    Sebastien Nisole
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Proc Natl Acad Sci U S A 101:13324-8. 2004
    ..The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling...
  69. pmc Structure-function analysis of the human JC polyomavirus establishes the LSTc pentasaccharide as a functional receptor motif
    Ursula Neu
    Interfaculty Institute for Biochemistry, University of Tubingen, D 72076 Tubingen, Germany
    Cell Host Microbe 8:309-19. 2010
    ..Our findings provide a powerful platform for the development of antiviral compounds...
  70. pmc Cooperative effect of the attenuation determinants derived from poliovirus sabin 1 strain is essential for attenuation of enterovirus 71 in the NOD/SCID mouse infection model
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Virol 82:1787-97. 2008
    ..These results suggest that a cooperative effect of the attenuation determinants of PV1(Sabin) is essential for attenuated neurovirulence of EV71...
  71. pmc The epithelial integrin alphavbeta6 is a receptor for foot-and-mouth disease virus
    T Jackson
    Department of Molecular Biology, Institute for Animal Health, Pirbright, Surrey GU24 ONF, United Kingdom
    J Virol 74:4949-56. 2000
    ..These studies establish a role for alphavbeta6 as a cellular receptor for FMDV...
  72. pmc Phylodynamic reconstruction reveals norovirus GII.4 epidemic expansions and their molecular determinants
    J Joukje Siebenga
    National Institute for Public Health and the Environment, RIVM, Bilthoven, The Netherlands
    PLoS Pathog 6:e1000884. 2010
    ..The observed pattern of continually emerging novel variants of GII.4, causing elevated numbers of infections, is therefore a cause for concern...
  73. pmc Incorporation of the green fluorescent protein into the herpes simplex virus type 1 capsid
    P Desai
    Virology Laboratories, Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 72:7563-8. 1998
    ....
  74. pmc Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5α-mediated restriction
    Ken Kono
    Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Retrovirology 7:72. 2010
    ..In contrast, rhesus monkey (Rh) TRIM5α could restrict all HIV-2 strains tested but not simian immunodeficiency virus isolated from macaque (SIVmac), despite its genetic similarity to HIV-2...
  75. pmc High frequencies of exposure to the novel human parvovirus PARV4 in hemophiliacs and injection drug users, as detected by a serological assay for PARV4 antibodies
    Colin P Sharp
    Centre for Infectious Diseases, University of Edinburgh, Edinburgh, United Kingdom
    J Infect Dis 200:1119-25. 2009
    ....
  76. ncbi Genetic analysis of the VP1 region of human enterovirus 71 strains isolated in Korea during 2000
    Y M Jee
    Department of Virology, National Institute of Health, Seoul, Korea
    Arch Virol 148:1735-46. 2003
    ..Analysis of the nucleotide sequences of the amplified fragments showed that the EV71 isolates from patients were over 98% homologous and belonged to the genotype C...
  77. ncbi Structural and functional analysis of prehistoric lentiviruses uncovers an ancient molecular interface
    David C Goldstone
    Division of Molecular Structure, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
    Cell Host Microbe 8:248-59. 2010
    ..Together, these data provide evidence for an ancient capsid-cyclophilin interaction preserved throughout lentiviral evolution...
  78. pmc Gangliosides are receptors for murine polyoma virus and SV40
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 22:4346-55. 2003
    ..Lipid binding sites for polyoma are shown to be present in rough ER membranes, suggesting that the virus travel with the ganglioside(s) from the plasma membranes to the ER...
  79. pmc Solution structure of dengue virus capsid protein reveals another fold
    Lixin Ma
    Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 101:3414-9. 2004
    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
  80. pmc VIPERdb2: an enhanced and web API enabled relational database for structural virology
    Mauricio Carrillo-Tripp
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nucleic Acids Res 37:D436-42. 2009
    ....
  81. pmc Structure of reovirus sigma1 in complex with its receptor junctional adhesion molecule-A
    Eva Kirchner
    Interfaculty Institute for Biochemistry, University of Tuebingen, Tuebingen, Germany
    PLoS Pathog 4:e1000235. 2008
    ..These studies define biophysical mechanisms of reovirus cell attachment and provide a platform for manipulating reovirus tropism to enhance vector targeting...
  82. pmc Target cell cyclophilin A modulates human immunodeficiency virus type 1 infectivity
    Elena Sokolskaja
    Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
    J Virol 78:12800-8. 2004
    ..Inhibition of HIV-1 infectivity resulting from virion production in the presence of CsA occurs independently of the CA-CypA interaction or even of CypA...
  83. ncbi Complete genome sequences of all members of the species Human enterovirus A
    M Steven Oberste
    Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G 17, Atlanta, GA 30333, USA
    J Gen Virol 85:1597-607. 2004
    ..CVA6-Gdula clearly contains sequences that are also present in CVA10-Kowalik and CVA12-Texas-12, suggesting that these three strains have a shared evolutionary history despite their lack of similarity in the capsid region...
  84. pmc Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease virus
    S Curry
    Pirbright Laboratory, Institute for Animal Health, Surrey, United Kingdom
    J Virol 71:9743-52. 1997
    ..A comparison of the putative active sites in FMDV and poliovirus suggests a structural explanation for the sequence specificity of the cleavage reaction...
  85. pmc Structural analysis of histo-blood group antigen binding specificity in a norovirus GII.4 epidemic variant: implications for epochal evolution
    Sreejesh Shanker
    Verna Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Virol 85:8635-45. 2011
    ....
  86. ncbi Capsid proteins from field strains of foot-and-mouth disease virus confer a pathogenic phenotype in cattle on an attenuated, cell-culture-adapted virus
    Anette Bøtner
    National Veterinary Institute, Technical University of Denmark, Lindholm, 4771 Kalvehave, Denmark
    J Gen Virol 92:1141-51. 2011
    ..Thus, the surface-exposed capsid proteins of the O1K B64 strain are responsible for its attenuation in cattle...
  87. pmc Hydrogen-bonding networks and RNA bases revealed by cryo electron microscopy suggest a triggering mechanism for calcium switches
    Peng Ge
    Department of Microbiology, Immunology, and Molecular Genetics and the California NanoSystems Institute, University of California, Los Angeles, CA 90095 7364, USA
    Proc Natl Acad Sci U S A 108:9637-42. 2011
    ..In turn, the coat protein-RNA interactions in our structure favor an adenine-X-guanine (A*G) motif over the G*A motif of the dormant state, thus offering an explanation underlying viral assembly initiation by an AAG motif...
  88. ncbi Insights into the role and function of L2, the minor capsid protein of papillomaviruses
    Ramon Pereira
    Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa
    Arch Virol 154:187-97. 2009
    ..However, its low abundance in natural capsids--12-72 molecules per 360 copies of L1--limits its immunogenicity. This review will explore the biological roles of the protein, and prospects for its use in new vaccines...
  89. ncbi Immunization of knock-out α/β interferon receptor mice against lethal bluetongue infection with a BoHV-4-based vector expressing BTV-8 VP2 antigen
    Valentina Franceschi
    Dipartimento di Salute Animale, Facolta di Medicina Veterinaria, Universita di Parma, Via del Taglio 10, 43100 Parma, Italy
    Vaccine 29:3074-82. 2011
    ..The data acquired in this pilot study validate BoHV-4-based vector as a safe and effective heterologous antigen carrier/producer for the formulation of enhanced recombinant immunogens for the vaccination against lethal bluetongue...
  90. ncbi Cancer-specific toxicity of apoptin is independent of death receptors but involves the loss of mitochondrial membrane potential and the release of mitochondrial cell-death mediators by a Nur77-dependent pathway
    Subbareddy Maddika
    Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, MB R3E OV9, Canada
    J Cell Sci 118:4485-93. 2005
    ..Thus, our data indicate that the apoptin death signal(s) ultimately converges at the mitochondria, and that it acts independently of the death receptor pathway...
  91. pmc Capsid-incorporation of antigens into adenovirus capsid proteins for a vaccine approach
    Qiana L Matthews
    Division of Human Gene Therapy, Departments of Medicine, Pathology, Surgery, Obstetrics and Gynecology, The Gene Therapy Center, and Center for AIDS Research, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Mol Pharm 8:3-11. 2011
    ..approach results in a strong humoral response, similar to the response generated by native Ad capsid proteins. This strategy also allows for the boosting of antigenic specific responses...
  92. pmc Change of major genotype of enterovirus 71 in outbreaks of hand-foot-and-mouth disease in Taiwan between 1998 and 2000
    Jen Ren Wang
    Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    J Clin Microbiol 40:10-5. 2002
    ..Furthermore, the predominant EV71 genotypes responsible for each of the two major HFMD outbreaks within the 3-year period in Taiwan were different...
  93. pmc A pan-HPV vaccine based on bacteriophage PP7 VLPs displaying broadly cross-neutralizing epitopes from the HPV minor capsid protein, L2
    Ebenezer Tumban
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico, United States of America
    PLoS ONE 6:e23310. 2011
    ..Here, we report the development of a panel of PP7 VLP-based vaccines targeting L2 that protect mice from infection with carcinogenic and non-carcinogenic HPV types that infect the genital tract and skin...
  94. pmc Role of multiple hosts in the cross-species transmission and emergence of a pandemic parvovirus
    Andrew B Allison
    Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
    J Virol 86:865-72. 2012
    ..This highlights the potential role of alternative hosts in viral emergence...
  95. pmc Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic
    R Kirnbauer
    Laboratory of Cellular Oncology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 89:12180-4. 1992
    ..We have expressed the L1 major capsid proteins of bovine papillomavirus type 1 and human papillomavirus type 16 in insect cells via a baculovirus vector and ..
  96. pmc Major and minor capsid proteins of human polyomavirus JC cooperatively accumulate to nuclear domain 10 for assembly into virions
    Yukiko Shishido-Hara
    Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Japan
    J Virol 78:9890-903. 2004
    ..The major capsid protein VP1, the minor capsid proteins VP2 and VP3, and a regulatory protein called agnoprotein were coexpressed from a polycistronic expression ..
  97. ncbi Characterization of infectious particles of grass carp reovirus by treatment with proteases
    Q Fang
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
    Arch Virol 153:675-82. 2008
    ..Overall, the results presented in this paper provided strong evidence that the proteins VP5 and VP7 of GCRV play an indispensable role in viral infection...
  98. pmc Rapid evolution of pandemic noroviruses of the GII.4 lineage
    Rowena A Bull
    School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney, New South Wales, Australia
    PLoS Pathog 6:e1000831. 2010
    ..This study supports the hypothesis that the ability of the virus to generate genetic diversity is vital for viral fitness...
  99. ncbi Reliable titration of filamentous bacteriophages independent of pIII fusion moiety and genome size by using trypsin to restore wild-type pIII phenotype
    Geir Åge Løset
    Department of Molecular Biosciences, University of Oslo, Oslo, Norway
    Biotechniques 44:551-2, 554. 2008
    ..The results show that the trypsin treatment has a very large but heterogeneous effect on the phage infection efficiency, depending on the pIII fusion domain and the valence of display...
  100. ncbi Evidence for the role of His-142 of protein 1C in the acid-induced disassembly of foot-and-mouth disease virus capsids
    F M Ellard
    Pirbright Laboratory, Institute for Animal Health, Surrey, UK
    J Gen Virol 80:1911-8. 1999
    ..by using a previously described vaccinia virus expression system, in which synthesis and processing of FMDV capsid proteins results in the self-assembly of capsids...
  101. pmc Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons
    G A Smith
    Department of Molecular Biology, Schultz Building, Room 301, Princeton University, Princeton, NJ 08544 1014, USA
    Proc Natl Acad Sci U S A 98:3466-70. 2001
    ..The observed movement was compatible with fast axonal flow mediated by multiple microtubule motors. Capsids accumulated at axon terminals, suggesting that spread from infected neurons required cell contact...

Research Grants70

  1. Nanopolymersomes for Transcutaneous Co-Delivery of Vaccine Antigens and Adjuvants
    PAUL CAMILLE TUMEH; Fiscal Year: 2013
    ..As a future independent investigator, this system will enable me to collaborate with scientists and clinicians to address a wide scope of challenges in medicine. ..
  2. Present Homologous and Heterologous Antigen with Hepatitis E Virus
    R Holland Cheng; Fiscal Year: 2012
    ..from human hepatitis E virus (HEV-VLP) is empty icosahedral cage composed of 60 copies of recombinant HEV capsid proteins. This VLP is capable of encapsulating DNA vaccine in vitro, delivering DNA vaccine to epithelium cell at ..
  3. CTL response to AAV Vector
    Richard J Samulski; Fiscal Year: 2013
    ..Through these studies, we expect to thoroughly characterize the CTL response to AAV capsid proteins in mice that more accurately represents data obtained in humans...
  4. Clinical trial of self complementary AAV8-mediated gene transfer for hemophilia B
    Andrew M Davidoff; Fiscal Year: 2013
    ..of hFIX at or above 3% of normal;2) to describe the immune responses to the hFIX transgene product and AAV capsid proteins and 3) to access viral shedding into various body fluids...
  5. STRUCTURAL STUDIES OF NUCLEOPROTEIN COMPLEXES
    Edward H Egelman; Fiscal Year: 2012
    ..IV) Capsid proteins that form spherical viral shells can be mutated or modified so that they form helical tubes instead...
  6. The Pox Virion Molecular Interactome
    Paul D Gershon; Fiscal Year: 2013
    ..The importance of virus envelopes and capsid proteins in mediating the effects of antiviral therapeutics and vaccines is undisputed...
  7. Theiler's virus-induced aoptosis: A mechanism for CNS virus persistence
    HOWARD LEE LIPTON; Fiscal Year: 2013
    ..TMEV nonstructural proteins L and 3CD in mammalian cells, and determine whether TMEV replicons in which the capsid proteins are deleted can induce apoptosis;and 4) investigate the mechanism of loss of infectious virus in murine ..
  8. The Evolutionary and Biological Bases of Host Switching in Viruses
    Colin R Parrish; Fiscal Year: 2013
    ..By revealing the evolutionary processes that underpin cross- species virus transmission and emergence we will establish general rules that will assist in the control and prevention of future emergent viruses. ..
  9. Humoral and cellular immunity in polyomavirus-linked Merkel cell carcinoma
    Paul Nghiem; Fiscal Year: 2013
    ....
  10. Picornavirus-host Cell Interactions and Disease
    Raymond Roos; Fiscal Year: 2009
    ..of DA that includes the 5'-untranslated region (UTR) and coding regions for the L, L*, and P1 (the capsid proteins) in myelinating cells develop white matter pathology;this study demonstrated that a direct effect of DA virus ..
  11. Optimized Adaptation of Simian-tropic R5 HIV Clade C to Pig-tailed Macaques
    RUTH MARGRIT RUPRECHT; Fiscal Year: 2013
    ..factors that block HIV-1 replication in RM cells have been recognized, namely 1) TRIM5?, which targets HIV-1 capsid proteins and blocks early post-entry replication steps, and 2) APOBEC3G (A3G), a cytidine deaminase that is not ..
  12. Novel vaccine against Norovirus
    Xi Jiang; Fiscal Year: 2013
    ..abstract_text> ..
  13. Inflammasome activation by a respiratory virus
    Christopher M Wiethoff; Fiscal Year: 2010
    ..We will determine the role of adenovirus capsid proteins in activating the inflammasome via NALP3 and the adaptor protein ASC...
  14. Astrovirus structure and replication
    Yizhi Jane Tao; Fiscal Year: 2013
    ..The structure of the complex will reveal whether the same catalytic mechanism is used for both VPg nucleotidylation and regular nucleotide polymerization. ..
  15. Protein-DNA Drug Carriers for Tumor Targeting
    Lali K Medina-Kauwe; Fiscal Year: 2012
    ..As HER2+ breast cancer does not respond well to conventional therapies, this alternative therapy could provide a significant contribution to breast cancer treatment, and could be modified to target therapy to other types of cancer. ..
  16. A Phase I Study to Evaluate the Safety and Immunogenicity of HPV 16 L2 11-200
    WARNER KING HUH; Fiscal Year: 2012
    ..The two HPV capsid proteins, L1 and L2, are both independent protective antigens...
  17. Interaction of Sindbis Virus with Cellular Processes
    ILYA V FROLOV; Fiscal Year: 2010
    ..Identification of the cellular proteins that are essential for virus growth will lead to development of new antiviral therapeutic strategies. ..
  18. Susceptibility and Protective Immunity to Noroviruses
    Ralph S Baric; Fiscal Year: 2013
    ....
  19. Newcastle Disease Virus Vectored Vaccines for Norovirus Infection
    Siba K Samal; Fiscal Year: 2013
    ..In this project, we propose to engineer recombinant NDVs individually expressing the capsid proteins of human and murine noroviruses and to evaluate their protective immunity against murine norovirus in mice...
  20. Release and trafficking of JC polyomavirus genomes and implications in AIDS
    CHRISTIAN DOUGLAS STUART NELSON; Fiscal Year: 2012
    ..This aim may provide a mechanistic understanding of release of minor capsid proteins and genome are from JCV and may lead to the development of antiviral compound that prevent the egress of the ..
  21. The roles of acidic autophagosomes in production of infectious poliovirus
    William T Jackson; Fiscal Year: 2013
    ..This work is also an important step in understanding the mechanisms of existing and future therapeutic agents against multiple viral diseases. ..
  22. Dengue Virus Genome Encapsidation and Its Interplay with Host Lipid Droplets
    Andrea Gamarnik; Fiscal Year: 2013
    ..Importantly, the studies proposed will provide new information about a viral process that has been unexplored for antiviral intervention. ..
  23. Adenovirus-based Anti-Cocaine Vaccine
    Ronald G Crystal; Fiscal Year: 2010
    ..The humoral adaptive immunity induced by Ad is directed against the capsid proteins, including 720 hexons and 96 combined penton bases and fibers, abundant targets for conjugating cocaine...
  24. Regulation of reovirus induced apoptosis
    John S Parker; Fiscal Year: 2012
    ....
  25. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2013
    ..Cellular proteins that are required for retroviral replication are potential targets for the development of new drugs to treat AIDS. ..
  26. ASSEMBLY OF THE HERPES SIMPLEX VIRUS CAPSID
    Jay C Brown; Fiscal Year: 2012
    ..that assembly involves a filamentous aggregate of the scaffolding protein bound to the portal and major capsid proteins (aim 3). This is proposed to condense in a stepwise fashion to create the procapsid...
  27. Identification of novel HIV capsid assembly inhibitors (Hit-to-Lead development)
    OSVALDO ARIEL FLORES; Fiscal Year: 2011
    ..Molecules that target HIV Capsid assembly offer a unique promise of providing high genetic barrier to resistance as well as complement existing therapies as part of combination therapy. ..
  28. Multivalent Plague, Anthrax Vaccines Using Bacteriophage T4 Display
    Venigalla B Rao; Fiscal Year: 2013
    ..Full-length anthrax toxins, as large as 90kDa, are fused to the two non-essential phage T4 outer capsid proteins, Soc (small outer capsid protein;870 copies) and Hoc (highly antigenic outer capsid protein;155 copies), over-..
  29. IMMUNITY AND PATHOGENESIS OF A NOVEL NOROVIRUS
    Herbert W Virgin; Fiscal Year: 2012
    ..significant insight has come from studies of the immune response to virus-like-particles generated when NoV capsid proteins are over expressed...
  30. Electron microscopy studies of novel poliovirus genome-releasing intermediates
    Hazel C Levy; Fiscal Year: 2010
    ..They rely on the ability of capsid proteins to undergo as-yet-uncharacterized conformational changes for the viral RNA to breach the host-membrane ..
  31. Tailoring AAV Vectors for Glioma Immunotherapy
    Ou Cao; Fiscal Year: 2011
    ....
  32. Molecular biology of TSST-1 and other superantigen toxins
    Richard P Novick; Fiscal Year: 2012
    ..more phage functions to inactivate its repressor, resulting in excision and replication;it remodels the phage capsid proteins to form its specific small particles;it diverts the phage packaging system to promote packaging of SaPI ..
  33. Mechanism of Folate Deficiency as a Co-Factor for HPV16-induced Carcinogenesis
    ASOK ANTONY; Fiscal Year: 2013
    ..led to a profound perturbation in the generation of both HPV16 major (L1) and minor (L2) viral capsid proteins in vitro;in HPV16-harboring keratinocytes that were propagated as monolayers;as well as when these HPV16-..
  34. Exploration of novel pan-HPV treatments to block development of AIDS-associated c
    Wijbe Martin Kast; Fiscal Year: 2010
    ....
  35. Oral Immunization Against Malaria with Recombinant Adenoviruses
    GARY W KETNER; Fiscal Year: 2010
    ..Antigens will be produced either as fusions with major viral capsid proteins designed to display antigenic epitopes on the surface of the recombinant particles (capsid display ..
  36. Mechanisms in Polyoma and Papillomavirus Assembly
    ROBERT GARCEA; Fiscal Year: 2013
    ..We hypothesize that cellular chaperone proteins, specifically the hsc70 family, maintain the viral capsid proteins in an unassembled form until they encounter viral genomes, which have been specifically marked by a ..
  37. Potent Phage T4 Derived V2 Immunogens as HIV Vaccines
    Venigalla B Rao; Fiscal Year: 2013
    ..These might potentially serve as preventative HIV-1 vaccine candidates for further studies in nonhuman primates and clinical trials. ..
  38. Next Generation of Recombinant AAV Serotype Vectors for Gene Therapy
    Sergei Zolotukhin; Fiscal Year: 2013
    ....
  39. Molecular biology of viral capsids
    CHENG C KAO; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Viral capsid proteins are important regulators of viral processes from translation, to RNA replication, to RNA packaging, and the modulation of cellular innate immune responses...
  40. Structure and function of icosahedral viruses
    Michael G Rossmann; Fiscal Year: 2013
    ..We have also initiated X-ray crystaliographic studies of the major capsid proteins of these viruses as well as specific viral proteins such as the unique glycosyltransferases that modify the ..
  41. Regulation of Rotavirus Replication
    MARY KOLB ESTES; Fiscal Year: 2013
    ..The transient envelope is lost in a process involving rearrangement and assembly of the outer capsid proteins. The budding process involves interaction of the nascent subviral particles with the unique RV non-structural ..
  42. Second-generation Gene Transfer Vector-based Anti-cocaine Vaccines
    Ronald G Crystal; Fiscal Year: 2013
    ..Ad is a potent adjuvant that activates the immune system, we hypothesized that cocaine analogs linked to Ad capsid proteins would elicit high level, high affinity cocaine specific antibodies sufficient to trat cocaine addiction...
  43. Crystal structures and mechanism of disease of the astrovirus capsid protein
    REBECCA MICHELLE DUBOIS; Fiscal Year: 2013
    ..These studies will demonstrate for the first time the mechanism of astrovirus entry into host cells and mechanism of astrovirus disease. ..
  44. DNA REPLICATION AND GENE EXPRESSION OF CHLORELLA VIRUSES
    James Van Etten; Fiscal Year: 2009
    ..Thus, chlorella viruses are an important model for studying large nucleocytoplasmic dsDNA viruses because they have evolutionary ties to important mammalian viruses, such as the pox viruses and the quarantined African swine fever virus. ..
  45. Mechanisms and Functions of Core-induced Lipid Droplet Accumulation in Hepatitis
    MELANIE MARIA OTT; Fiscal Year: 2010
    ..These studies are directly relevant to HCV pathogenesis and may contribute to the development of novel antiviral drugs that will address public need. ..
  46. Structures of the portal vertex in dsDNA viruses and virus-infected cells
    Wah Chiu; Fiscal Year: 2011
    ..Our goal is to determine the structure and functional role for each of the capsid proteins involved in capsid assembly and DNA injection, including the non-icosahedral components whose structures have ..
  47. Development of Human beta cell-specific AAV Vectors for Type I Diabetes
    Roland M Tisch; Fiscal Year: 2013
    ..A two-pronged approach will be taken. First, we will employ a panel of wild-type AAV capsid proteins to identify those capsids that efficiently transduce human [unreadable] cells in vitro, and in an in vivo ..
  48. Optimizing adenoviral vector to elicit a potent anti-malaria immunity
    Moriya Tsuji; Fiscal Year: 2012
    ..Because Ad is known to elicit a strong pre-existing anti-Ad immunity that is primarily against its capsid proteins, we hypothesized that the insertion of a foreign epitope into the capsid proteins results in an induction of ..
  49. Development of EGFR-Targeted Oncolytic Adenovirus for Therapy of Oral Cancers
    Anton V Borovjagin; Fiscal Year: 2012
    ....
  50. Computational modeling of viral assembly: encapsulation of nucleic acids and env
    Michael F Hagan; Fiscal Year: 2013
    ..Therefore, this project develops and applies computational models for capsid proteins, NAs, and lipids that reveal details of assembly and membrane budding not accessible to experiments...
  51. Development of low cost and broadly protective human papillomavirus vaccines
    RICHARD BRUCE RODEN; Fiscal Year: 2010
    ....
  52. Towards a Vaccine for the Common Cold
    THOMAS JAMES SMITH; Fiscal Year: 2010
    ..Previous studies have identified the major antigenic sites on the exterior of the capsid proteins. However, these proteins cannot be expressed in a soluble form and none of the current neutralizing ..
  53. Parvovirus RNA Processing and Gene Expression Strategies
    David J Pintel; Fiscal Year: 2013
    ..of partially-processed MVC capsid gene mRNAs that retain that site, and thus for the production of the capsid proteins. This represents a novel way in which this parvovirus regulates access to capsid gene information...
  54. Viral Constraints on SIV Escape from Cytotoxic T Lymphocytes
    WENDY WEN LI YEH; Fiscal Year: 2010
    ..6P in vivo. Ultimately, these studies will facilitate the design of novel vaccination strategies that may prevent the evolution of viral escape mutations. ..
  55. LIGHT-ACTIVATED GENE EXPRESSION IN SINGLE CELLS WITHIN TISSUE
    Robert H Singer; Fiscal Year: 2011
    ..that controls how and where other proteins are made in the cell. We are particularly interested in proteins that cause cancer, oncogenes, and in determining how these proteins can induce cells to produce tumors. ..
  56. Nuclear Import Pathways for HPV Macromolecules
    JUNONA F MOROIANU; Fiscal Year: 2010
    ..Nuclear import of HPV capsid proteins and genome is crucial for papillomavirus replication cycle...
  57. ALPHA-V INTEGRINS AND ADENOVIRUS CELL ENTRY
    Glen R Nemerow; Fiscal Year: 2013
    ..adenovirus at near atomic resolution thereby revealing the locations, folds and interactions of several viral capsid proteins. Additional investigations in the Nemerow and Stewart laboratories (Vanderbilt) discovered that specific host ..
  58. Role of Cellular Factors in Retroviral Uncoating and Synthesis of Viral DNA
    Felipe Diaz-Griffero; Fiscal Year: 2013
    ....
  59. Mechanisms of Norovirus Entry
    Christiane Wobus; Fiscal Year: 2012
    ..Therefore, our goal is to improve our understanding of the role of both the host and the virus in these early events during norovirus infection in the hopes of identifying new targets for antiviral drug development. ..
  60. Preclinical &Phase I/II Trials of AAV-AAT Vectors
    Terence R Flotte; Fiscal Year: 2013
    ..This model will allow us to characterize immune responses by measuring ELISPOT responses to AAV9 capsid proteins and to AAT transgene product as well as antibody formation against rAAV9 capsid...
  61. HPV VLP AND ANTIGEN PRESENTING CELLS
    Wijbe Martin Kast; Fiscal Year: 2013
    ....
  62. Virus-Like Particle Vaccine for SARS-CoV
    RAPHAEL PAUL VISCIDI; Fiscal Year: 2010
    ..The capsid proteins of viruses typically self-assemble into empty capsids known as virus-like particles (VLPs)...
  63. Nonstructural roles of capsid proteins in virus replication
    TERYL FREY; Fiscal Year: 2009
    ..b>Capsid proteins of other medically important viruses may play a similar role...
  64. Transduction of the Mouse Auditory System with AAV
    DIANA LURIE; Fiscal Year: 2003
    ..Importantly, 6 different AAV serotypes have been isolated (AAV 1-6) which differ in their respective capsid proteins. Consequently these serotypes exhibit moderate to substantial differences in the efficiency of transduction ..
  65. MECHANISMS OF PARVOVIRUS INFECTION AND HOST RANGE
    Colin R Parrish; Fiscal Year: 2011
    ..of related parvoviruses which differ in host range as a result of a small number of differences in their capsid proteins. Canine parvovirus (CPV) emerged in 1978 as a variant of feline panleukopenia virus (FPV), and then underwent ..
  66. Structural Basis for Long-Range Genetic Interactions in Retroviral CA Assembly
    Rebecca Craven; Fiscal Year: 2009
    ..A detailed understanding of this essential step of virus infection will allow development of better maturation inhibitors for use as antiretroviral drugs. ..
  67. NONINVASIVE DETECTION OF ANTIBODIES AGAINST HPV
    Michael Hagensee; Fiscal Year: 2000
    ..This proposal will lay the groundwork for the application of a saliva test to perform large epidemiological surveys and better define the populations at risk for HPV infection and disease. ..
  68. STRUCTURAL STUDIES OF PAPILLOMA VIRUSES
    Xiaojiang Chen; Fiscal Year: 2005
    ....
  69. Enhancing Gene Therapy by Designing Chimeric AAV Virions
    JARROD JOHNSON; Fiscal Year: 2009
    ..One of three capsid proteins, viral protein 1 (VP1), contains a unique part (VP1up) that possesses a putative phospholipase motif and ..
  70. STRUCTURAL STUDIES OF PROTEIN SUBUNITS
    Thomas Smith; Fiscal Year: 2004
    ..conformational changes in the virion, and the viral usurping of normal cellular functions via a number of non-capsid proteins. The dominant dogma is that some of these viruses have evolved 'canyon' regions to protect the receptor-..