viral structural proteins

Summary

Summary: Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS.)

Top Publications

  1. ncbi Rabies virus chaperone: identification of the phosphoprotein peptide that keeps nucleoprotein soluble and free from non-specific RNA
    Manos Mavrakis
    EMBL Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
    Virology 349:422-9. 2006
  2. ncbi Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus
    C N Wu
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC
    Vaccine 20:895-904. 2001
  3. ncbi The molecular basis of mouse adaptation by human enterovirus 71
    Beng Hooi Chua
    Division of Virology, Telethon Institute for Child Health Research, Perth, Australia
    J Gen Virol 89:1622-32. 2008
  4. ncbi Combined peptides of human enterovirus 71 protect against virus infection in mice
    Jiang Ning Liu
    Key Laboratory of Human Diseases Comparative Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences CAMS and Comparative Medicine Centre, Peking Union Medical Collage PUMC, Beijing 100021, China ljn
    Vaccine 28:7444-51. 2010
  5. pmc A pandemic strain of calicivirus threatens rabbit industries in the Americas
    Michael T McIntosh
    Foreign Animal Disease Diagnostic Laboratory, Animal and Plant Health Inspection Services, United States Department of Agriculture, Plum Island Animal Disease Center, P, O, Box 848, Greenport, NY 11944, USA
    Virol J 4:96. 2007
  6. ncbi A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant
    L Capucci
    Department of Biotechnology, Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia, Brescia, Italy
    Virus Res 58:115-26. 1998
  7. pmc Complete genome characterisation of a novel 26th bluetongue virus serotype from Kuwait
    Sushila Maan
    Vector Borne Diseases Programme, Institute for Animal Health, Pirbright, Woking Surrey, United Kingdom
    PLoS ONE 6:e26147. 2011
  8. ncbi The multipartite system that mediates entry of herpes simplex virus into the cell
    Gabriella Campadelli-Fiume
    Department of Experimental Pathology, Section on Microbiology and Virology, Alma Mater Studiorum, University of Bologna, Bologna, Italy
    Rev Med Virol 17:313-26. 2007
  9. pmc Epidemiology and genetic characterization of hepatitis A virus genotype IIA
    Delphine Desbois
    centre national de Référence pour les virus des hépatites à transmission entérique, AP HP, Hopital Paul Brousse, Villejuif 94804, France
    J Clin Microbiol 48:3306-15. 2010
  10. pmc Emergence of a new lagovirus related to Rabbit Haemorrhagic Disease Virus
    Ghislaine Le Gall-Reculé
    Anses, French Agency for Food, Environmental and Occupational Health and Safety, Ploufragan Plouzane Laboratory, Avian and Rabbit Virology, Immunology and Parasitology Unit, BP 53, 22440 Ploufragan, France
    Vet Res 44:81. 2013

Research Grants

  1. CTL response to AAV Vector
    Richard J Samulski; Fiscal Year: 2013
  2. Molecular Biology of Eukaryotic Viruses
    Bert L Semler; Fiscal Year: 2012
  3. Theiler?s virus as a potential cause of Vilyuisk encephalitis
    HOWARD LEE LIPTON; Fiscal Year: 2010
  4. Viral Gastroenteritis: Basis of Protection and Virulence
    HARRY BERNARD GREENBERG; Fiscal Year: 2013
  5. Newcastle Disease Virus Vectored Vaccines for Norovirus Infection
    Siba K Samal; Fiscal Year: 2013
  6. Rotavirus: Studies of Intestinal Tropism, Viral Entry, and Rescue
    HARRY BERNARD GREENBERG; Fiscal Year: 2013
  7. Mechanisms in Polyoma and Papillomavirus Assembly
    ROBERT GARCEA; Fiscal Year: 2013
  8. Control of Viral Pathogenesis by Regulation of 2-5A Levels
    Susan R Weiss; Fiscal Year: 2013
  9. Next Generation of Recombinant AAV Serotype Vectors for Gene Therapy
    Sergei Zolotukhin; Fiscal Year: 2013
  10. STRUCTURE AND ASSEMBLY OF VIRUSES
    STEPHEN COPLAN HARRISON; Fiscal Year: 2012

Detail Information

Publications352 found, 100 shown here

  1. ncbi Rabies virus chaperone: identification of the phosphoprotein peptide that keeps nucleoprotein soluble and free from non-specific RNA
    Manos Mavrakis
    EMBL Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
    Virology 349:422-9. 2006
    ..Coexpression and coimmunoprecipitation experiments and yeast two-hybrid experiments showed that this peptide alone could bind to N in vivo...
  2. ncbi Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus
    C N Wu
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC
    Vaccine 20:895-904. 2001
    ..Our data indicated that inactivated virus is the choice of vaccine preparation capable of fulfilling the demand for effective control, and that VP1 subunit vaccines remain promising vaccine strategies that require further refinement...
  3. ncbi The molecular basis of mouse adaptation by human enterovirus 71
    Beng Hooi Chua
    Division of Virology, Telethon Institute for Child Health Research, Perth, Australia
    J Gen Virol 89:1622-32. 2008
    ..Of the two amino acid substitutions identified in MP-26M, the VP1 (G(145)-->E) mutation alone was sufficient to increase virulence in mice to the level observed in MP-26M-infected mice...
  4. ncbi Combined peptides of human enterovirus 71 protect against virus infection in mice
    Jiang Ning Liu
    Key Laboratory of Human Diseases Comparative Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences CAMS and Comparative Medicine Centre, Peking Union Medical Collage PUMC, Beijing 100021, China ljn
    Vaccine 28:7444-51. 2010
    ..Our results suggested that Vac6 could have potential clinical value against EV71 epidemics caused mainly by C4 strains in the mainland of China...
  5. pmc A pandemic strain of calicivirus threatens rabbit industries in the Americas
    Michael T McIntosh
    Foreign Animal Disease Diagnostic Laboratory, Animal and Plant Health Inspection Services, United States Department of Agriculture, Plum Island Animal Disease Center, P, O, Box 848, Greenport, NY 11944, USA
    Virol J 4:96. 2007
    ..Given its rapid spread, pathogenic nature, and potential to further evolve, possibly broadening its host range to include other genera native to the Americas, RHDVa should be regarded as a threat...
  6. ncbi A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant
    L Capucci
    Department of Biotechnology, Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia, Brescia, Italy
    Virus Res 58:115-26. 1998
    ..Bs89 drops to 70%, suggesting that this region probably contains the epitope recognized by mAb 1H8. In addition, this paper presents preliminary data concerning the amino acids of VP60 involved in the hemagglutination site of the virus...
  7. pmc Complete genome characterisation of a novel 26th bluetongue virus serotype from Kuwait
    Sushila Maan
    Vector Borne Diseases Programme, Institute for Animal Health, Pirbright, Woking Surrey, United Kingdom
    PLoS ONE 6:e26147. 2011
    ..Our analyses indicate that all of the BTV genome segments have evolved under strong purifying selection...
  8. ncbi The multipartite system that mediates entry of herpes simplex virus into the cell
    Gabriella Campadelli-Fiume
    Department of Experimental Pathology, Section on Microbiology and Virology, Alma Mater Studiorum, University of Bologna, Bologna, Italy
    Rev Med Virol 17:313-26. 2007
    ..Whether fusion execution is carried out by gB or gH.gL, or both glycoproteins in complex or sequentially remains to be determined...
  9. pmc Epidemiology and genetic characterization of hepatitis A virus genotype IIA
    Delphine Desbois
    centre national de Référence pour les virus des hépatites à transmission entérique, AP HP, Hopital Paul Brousse, Villejuif 94804, France
    J Clin Microbiol 48:3306-15. 2010
    ..However, the unexplained contamination mode of autochthonous cases suggests another, still to be discovered geographical origin or a French reservoir to be explored...
  10. pmc Emergence of a new lagovirus related to Rabbit Haemorrhagic Disease Virus
    Ghislaine Le Gall-Reculé
    Anses, French Agency for Food, Environmental and Occupational Health and Safety, Ploufragan Plouzane Laboratory, Avian and Rabbit Virology, Immunology and Parasitology Unit, BP 53, 22440 Ploufragan, France
    Vet Res 44:81. 2013
    ..RHDV2 was detected in continental Italy in June 2011, then four months later in Sardinia. ..
  11. ncbi Versatile RHDV virus-like particles: incorporation of antigens by genetic modification and chemical conjugation
    Matthew Peacey
    Department of Microbiology and Immunology, School of Medical Sciences, University of Otago, P O Box 56, Dunedin, 9054, New Zealand
    Biotechnol Bioeng 98:968-77. 2007
    ..VLP facilitated delivery of conjugated antigen to dendritic cells, eliciting proliferative responses in naïve TCR transgenic T helper cells that were at least 10-fold greater than ovalbumin antigen delivered alone...
  12. pmc Identification and characterization of viral structural proteins of infectious salmon anemia virus
    Knut Falk
    Section for Fish Health, National Veterinary Institute, Oslo, Austria
    J Virol 78:3063-71. 2004
    ..It is proposed that the ISAV vp66 protein be termed nucleoprotein, the gp42 protein be termed HE protein, and the vp22 protein be termed matrix protein...
  13. pmc Full genome sequencing and genetic characterization of Eubenangee viruses identify Pata virus as a distinct species within the genus Orbivirus
    Manjunatha N Belaganahalli
    Vector Borne Viral Diseases Programme, Institute for Animal Health, Woking, Surrey, United Kingdom
    PLoS ONE 7:e31911. 2012
    ..The data presented will facilitate diagnostic assay design and the identification of additional isolates of these viruses...
  14. ncbi Phylogenetic analysis of rabbit hemorrhagic disease virus in China and the antigenic variation of new strains
    Xinglong Wang
    College of Veterinary Medicine, Northwest A and F University, Yangling 712100, People s Republic of China
    Arch Virol 157:1523-30. 2012
    ..The present work is the first comprehensive analysis of Chinese RHDV and reveals a new RHDV variation that should be carefully monitored...
  15. ncbi Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus
    Beata Gromadzka
    Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland
    Acta Biochim Pol 53:371-6. 2006
    ..After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV...
  16. ncbi Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6A resolution: implications in virion assembly and immunogenicity
    Cheng Chung Lee
    Structural Biology Program, Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei 11221, Taiwan, ROC
    J Struct Biol 155:74-86. 2006
    ..These loops are targets of the neutralizing antibodies. Several common interactions between the surface loops were clearly observed, suggesting a possible major conformation of the immunogenic epitopes...
  17. ncbi Initiation of encapsidation as evidenced by deoxycholate-treated Nucleocapsid protein in the Chandipura virus life cycle
    Raja Bhattacharya
    Dr B C Guha Centre for Genetic Engineering and Biotechnology, Department of Biochemistry, University College of Science, University of Calcutta, Calcutta 700 019, India
    Virology 349:197-211. 2006
    ..Based on our observations, we propose a model to explain encapsidation that involves two discrete biochemically separable steps, initiation and elongation...
  18. pmc Rescue of very virulent and mosaic infectious bursal disease virus from cloned cDNA: VP2 is not the sole determinant of the very virulent phenotype
    H J Boot
    Department of Avian Virology, Institute for Animal Science and Health, Lelystad, The Netherlands
    J Virol 74:6701-11. 2000
    ..However, the very virulent phenotype was not solely determined by this protein, since mosaic virus containing VP2 of vvIBDV induced neither morbidity nor mortality in young chickens...
  19. ncbi Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus
    Zhongjun Hu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Protein Cell 1:48-58. 2010
    ..Collectively, our cryo-electron microscopic studies of RHDV provide close insight into the structure of Lagovirus, which is important for functional analysis and better vaccine development in the future...
  20. pmc Tegument proteins of human cytomegalovirus
    Robert F Kalejta
    Institute for Molecular Virology and McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, Wisconsin, USA
    Microbiol Mol Biol Rev 72:249-65, table of contents. 2008
    ..Important directions for future investigation and the challenges that lie ahead are identified and discussed...
  21. ncbi The solution structure of bacteriophage lambda protein W, a small morphogenetic protein possessing a novel fold
    K L Maxwell
    Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada
    J Mol Biol 308:9-14. 2001
    ..The 14 C-terminal residues of gpW, which are essential for function, are unstructured in solution...
  22. pmc Evolutionary relationships and systematics of the alphaviruses
    A M Powers
    Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA
    J Virol 75:10118-31. 2001
    ..Trocara virus, isolated from mosquitoes in Brazil and Peru, also represents a new species and probably a new alphavirus complex...
  23. pmc Peptides that mimic the amino-terminal end of the rabies virus phosphoprotein have antiviral activity
    Guillaume Castel
    Unité Postulante des Stratégies Antivirales, CNRS URA 3015, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Virol 83:10808-20. 2009
    ..In neuronal cells, a significant inhibition of both nucleocapsid inclusions and rabies virus release was observed...
  24. pmc Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation
    Xin Zhang
    Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA
    J Virol 82:674-82. 2008
    ..These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA...
  25. ncbi GP3 is a structural component of the PRRSV type II (US) virion
    M De Lima
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, 111 K Morrison Life Science Research Center Morrison Center, East Campus, Lincoln, NE 68583 0900, USA
    Virology 390:31-6. 2009
    ..Taken together, these results indicate that GP3 is a minor structural component of the PRRSV type II (FL12 strain) virion, as had been previously described for PRRSV type I...
  26. pmc Differing roles of inner tegument proteins pUL36 and pUL37 during entry of herpes simplex virus type 1
    Ashley P E Roberts
    MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, United Kingdom
    J Virol 83:105-16. 2009
    ..Analysis of noninfectious enveloped particles (L-particles) further showed that pUL36 and pUL37 are dependent on each other for incorporation into tegument...
  27. pmc Polyclonal antibody against the DPV UL46M protein can be a diagnostic candidate
    Liting Lu
    Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, YA, AN, Sichuan, China
    Virol J 7:83. 2010
    ..Subsequently, we established Dot-ELISA using the polyclonal antibody and applied it to DPV detection...
  28. pmc Expression and intracellular localization of duck enteritis virus pUL38 protein
    Jun Xiang
    Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan, Sichuan, China
    Virol J 7:162. 2010
    ..Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38...
  29. pmc Divergent and convergent evolution after a common-source outbreak of hepatitis C virus
    Stuart C Ray
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    J Exp Med 201:1753-9. 2005
    ..These data suggest that vaccines for genetically diverse viruses may be more effective if they represent consensus sequence, rather than a human isolate...
  30. ncbi Recombinant baculoviruses as mammalian cell gene-delivery vectors
    Thomas A Kost
    Gene Expression and Protein Biochemistry, GlaxoSmithKline Discovery Research, Research Triangle Park, North Carolina 27709, USA
    Trends Biotechnol 20:173-80. 2002
    ..These attributes will undoubtedly lead to the increased application and continued development of this system for efficient gene delivery into mammalian cells. Who said you can't teach an old dog new tricks?..
  31. ncbi Modular organization of rabies virus phosphoprotein
    Francine C A Gerard
    UJF EMBL CNRS UMI 3265 Unit of Virus Host Cell Interactions, Grenoble, France
    J Mol Biol 388:978-96. 2009
    ..Our results suggest a conserved organization of P proteins in the Rhabdoviridae family in concatenated functional domains resembling that of the P proteins in the Paramyxoviridae family...
  32. pmc Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains
    Jelle Matthijnssens
    Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Virol 82:3204-19. 2008
    ..These close evolutionary links between human and animal rotaviruses emphasize the need for close simultaneous monitoring of rotaviruses in animals and humans...
  33. pmc Crystal structure of an Aquabirnavirus particle: insights into antigenic diversity and virulence determinism
    Fasseli Coulibaly
    CNRS INRA UMR 2472 1157 and IFR 115, Laboratoire de Virologie Moleculaire et Structurale, 1 Avenue de la Terrasse, F 91198 Gif sur Yvette Cedex, France
    J Virol 84:1792-9. 2010
    ..Thus, in addition to revealing the viral antigenic determinants, the structure suggests that birnaviruses interact with different receptors for attachment and for cell internalization during entry...
  34. ncbi Reduced positive selection in vector-borne RNA viruses
    Christopher H Woelk
    Mol Biol Evol 19:2333-6. 2002
  35. ncbi Evidence for quasispecies distributions in the human hepatitis A virus genome
    Gloria Sanchez
    Grup Virus Entèrics, Department of Microbiology, University of Barcelona, 08028 Barcelona, Spain
    Virology 315:34-42. 2003
    ..The results indicate that despite antigenic conservation, HAV replicates as a complex distribution of mutants, a feature of viral quasispecies...
  36. ncbi Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3
    Shengwang Liu
    Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Gene 412:12-25. 2008
    ....
  37. ncbi Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera
    Johannes A Jehle
    Laboratory for Biotechnological Crop Protection, Department of Phytopathology, Agricultural Service Center Palatinate DLR Rheinpfalz, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany
    Virology 346:180-93. 2006
    ....
  38. ncbi Genetic variability of hepatitis A virus in South America reveals heterogeneity and co-circulation during epidemic outbreaks
    M Costa-Mattioli
    Laboratorie de Virologie, Institut de Biologie, Centre Hospitalier Regional Universitaire de Nantes, Rue Quai Moncousu 9, 44093 Nantes, France
    J Gen Virol 82:2647-52. 2001
    ..The genetic variability among strains isolated in this region seems to be higher in comparison with strains isolated in other regions of the world...
  39. ncbi Molecular and antigenic characterization of rabbit hemorrhagic disease virus isolated in Cuba indicates a distinct antigenic subtype
    O Farnos
    Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba
    Arch Virol 152:1215-21. 2007
    ..This is the second report on the molecular characterization of RHDVa circulating in the American region...
  40. pmc One misdated sequence of rabbit hemorrhagic disease virus prevents accurate estimation of its nucleotide substitution rate
    Allison L Hicks
    Department of Ecology, Evolution, and Natural Resources, School of Environmental and Biological Sciences Rutgers, The State University of New Jersey, 14 College Farm Rd, New Brunswick, NJ 08901, USA
    BMC Evol Biol 12:74. 2012
    ....
  41. ncbi Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome
    G Meyers
    Federal Research Centre for Virus Diseases of Animals, Tubingen, Federal Republic of Germany
    Virology 184:664-76. 1991
    ..Bacterial expression of sequences derived from the 3' part of the genomic RHDV RNA showed that this region codes for the viral capsid protein...
  42. pmc The respiratory syncytial virus M2-1 protein forms tetramers and interacts with RNA and P in a competitive manner
    Thi Lan Tran
    INRA, Unité de Virologie Immunologie Moléculaires UR892, F 78350 Jouy en Josas, France
    J Virol 83:6363-74. 2009
    ..We also show that the region encompassing amino acid residues 59 to 178 binds to P and RNA in a competitive manner that is independent of the phosphorylation status of M2-1...
  43. pmc Atomic model of rabbit hemorrhagic disease virus by cryo-electron microscopy and crystallography
    Xue Wang
    National Laboratory of Biomacromolecules, Institute of Biophysics IBP, Chinese Academy of Sciences CAS, Beijing, China
    PLoS Pathog 9:e1003132. 2013
    ..Our study provides a reliable, pseudo-atomic model of a Lagovirus and suggests a new candidate for an efficient vaccine that can be used to protect rabbits from RHDV infection...
  44. ncbi Sequence analysis of porcine kobuvirus VP1 region detected in pigs in Japan and Thailand
    Shoko Okitsu
    Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, 30 1, Oyaguchi kamicho, Itabashi, Tokyo 173 8610, Japan
    Virus Genes 44:253-7. 2012
    ..Since this strain was previously found to be closely related to bovine kobuviruses in the 3D gene region, it may be a natural recombinant...
  45. pmc Epidemics of enterovirus infection in Chungnam Korea, 2008 and 2009
    Kyoungah Baek
    Divison of Enteric and Hepatitis viruses, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea
    Virol J 8:297. 2011
    ..Comparison of the VP1 amino acid sequences of the Korean coxsackievirus B5 isolates with reference strains revealed amino acid sequence substitutions at nine amino acid sequences (532, 562, 570, 571, 576-578, 582, 583, and 585)...
  46. ncbi Hepatitis A virus genetic diversity in Venezuela: exclusive circulation of subgenotype IA and evidence of quasispecies distribution in the isolates
    Y Sulbaran
    Laboratorio de Virologia Molecular, CMBC, IVIC, Caracas, Venezuela
    J Med Virol 82:1829-34. 2010
    ..The molecular characterization of HAV isolates from Venezuela showed the circulation of a unique subgenotype IA, but with the presence of diverse strains and quasispecies inside the viral populations...
  47. ncbi Trout oral VP2 DNA vaccination mimics transcriptional responses occurring after infection with infectious pancreatic necrosis virus (IPNV)
    Natalia A Ballesteros
    Centro de Investigaciones Biologicas CSIC, Dpto Microbiología Molecular y Biología de la Infección, C Ramiro de Maeztu 9, 28040 Madrid, Spain
    Fish Shellfish Immunol 33:1249-57. 2012
    ..Some of the genes described here could be used as markers to further optimize the oral immunization method...
  48. pmc Structure of the vesicular stomatitis virus N⁰-P complex
    Cédric Leyrat
    UMI 3265 UJF EMBL CNRS Unit of Virus Host Cell Interactions, Grenoble, France
    PLoS Pathog 7:e1002248. 2011
    ..The structure of this complex also suggests a mechanism for the initiation of viral RNA synthesis...
  49. pmc Identification and characterization of vlf-1, a baculovirus gene involved in very late gene expression
    J R McLachlin
    Department of Entomology, University of Georgia, Athens 30602
    J Virol 68:7746-56. 1994
    ..The product of the newly identified gene has a surprising but significant relationship to a family of integrases and resolvases...
  50. ncbi The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment
    H J Boot
    Animal Sciences Group, Wageningen University and Research Centre, Lelystad, The Netherlands
    Arch Virol 150:137-44. 2005
    ..Insight into the virulence factors of very virulent IBDV will contribute to the improvement of live IBDV vaccines...
  51. pmc Complete, long-lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing VP2 antigens
    K Tsukamoto
    Department of Infectious Diseases, National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    J Virol 76:5637-45. 2002
    ....
  52. ncbi Protection of chickens, with or without maternal antibodies, against IBDV infection by a recombinant IBDV-VP2 protein
    Xuemei Zhou
    College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
    Vaccine 28:3990-6. 2010
    ....
  53. pmc Crystallographic insights into the autocatalytic assembly mechanism of a bacteriophage tail spike
    Ye Xiang
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Mol Cell 34:375-86. 2009
    ..Structural and sequence comparisons suggest that some eukaryotic and bacterial viruses as well as bacterial adhesins might have a similar maturation mechanism as is performed by phi29 gp12 for Bacillus subtilis...
  54. ncbi VP1 protein of infectious bursal disease virus modulates the virulence in vivo
    Meihong Liu
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    Virology 330:62-73. 2004
    ..Taken together, our results demonstrate that the VP1 protein of IBDV is involved in the efficiency of viral replication and modulates the virulence in vivo...
  55. ncbi Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens
    Jianxiang Wu
    State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 31009, China
    Plant Biotechnol J 5:570-8. 2007
    ..These results demonstrate that transgenic rice seeds expressing IBDV VP2 can be used as an effective, safe and inexpensive vaccine against IBDV...
  56. ncbi Protection of chickens against very virulent infectious bursal disease virus (IBDV) and Marek's disease virus (MDV) with a recombinant MDV expressing IBDV VP2
    K Tsukamoto
    Department of Virology, National Institute of Animal Health, Tsukuba, Japan
    Virology 257:352-62. 1999
    ..These results demonstrate that an effective and safe recombinant herpesvirus-based IBD vaccine could be constructed by expressing the VP2 antigen at the US2 site of the CVI-988 vaccine strain...
  57. ncbi The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid
    Juan Bárcena
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, 28130, Madrid, Spain
    Virology 322:118-34. 2004
    ..Mutant coat protein lacking this N-terminal sequence assembles into T = 1 capsids. Our results suggest that the polymorphism of the RHDV T = 3 capsid might bear resemblance to that of plant virus T = 3 capsids...
  58. ncbi Molecular epidemiological studies show that hepatitis A virus is endemic among active homosexual men in Europe
    Kathrine Stene-Johansen
    Division of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway
    J Med Virol 79:356-65. 2007
    ....
  59. ncbi Leader RNA binding ability of Chandipura virus P protein is regulated by its phosphorylation status: a possible role in genome transcription-replication switch
    Soumen Basak
    Dr B C Guha Centre for Genetic Engineering and Biotechnology, Department of Biochemistry, Calcutta University, Kolkata, India
    Virology 307:372-85. 2003
    ....
  60. ncbi Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
    S Yuan
    Department of Veterinary Pathobiology, 205 Veterinary Science Building, University of Minnesota, 1971 Commonwealth Avenue, 55108, St. Paul, MN, USA
    Virus Res 79:189-200. 2001
    ..The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system...
  61. ncbi Effect of osmolytes and chaperone-like action of P-protein on folding of nucleocapsid protein of Chandipura virus
    A Majumder
    Department of Biochemistry and the Dr. B. C. Guha Centre for Genetic Engineering and Biotechnology, University College of Science, University of Calcutta, 35 Ballygunge Circular Road, Calcutta 700 019, India
    J Biol Chem 276:30948-55. 2001
    ..The function of the phosphoprotein and osmolyte appears to be synergistic to keep the N-protein in a soluble biologically competent form in virus-infected cells...
  62. ncbi Antigenic structure of flavivirus proteins
    John T Roehrig
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
    Adv Virus Res 59:141-75. 2003
    ..To prepare effective and inexpensive subunit vaccines, we will need to continue to better understand these structure-function relationships of flavivirus proteins...
  63. pmc Herpes simplex virus tegument protein US11 interacts with conventional kinesin heavy chain
    Russell J Diefenbach
    Centre for Virus Research and Electron Microscopy Unit, The Westmead Millennium Institute, Westmead Hospital and University of Sydney, Westmead, New South Wales, Australia
    J Virol 76:3282-91. 2002
    ..These observations suggest a major role for the uKHC-US11 interaction in anterograde transport of unenveloped HSV nucleocapsids in axons...
  64. pmc Rabies virus P protein interacts with STAT1 and inhibits interferon signal transduction pathways
    Aurore Vidy
    Unité Mixte de Virologie Moléculaire et Structurale UMR 2472, CNRS, 91198 Gif sur Yvette Cedex, France
    J Virol 79:14411-20. 2005
    ..These results indicate that rabies P protein overcomes the antiviral response of the infected cells...
  65. ncbi The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions
    B Michel
    Immunology Vaccinology B43b, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B 4000 Liege, Belgium
    J Gen Virol 91:452-62. 2010
    ..The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus...
  66. ncbi Enteroviruses in Tunisia: virological surveillance over 12 years (1992-2003)
    O Bahri
    Laboratory of Clinical Virology, WHO Regional Reference Laboratory of Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunis Belvedere, Tunisia
    J Med Microbiol 54:63-9. 2005
    ..This study is the first report of the epidemiology of NPEV in Tunisia. These viruses are associated with various diseases and epidemiological data may help to clarify their impact on human health...
  67. pmc Genomic analysis of Clostridium perfringens bacteriophage phi3626, which integrates into guaA and possibly affects sporulation
    Markus Zimmer
    Institut fur Mikrobiologie, FML Weihenstephan, Technische Universitat Munchen, D 85350 Freising, Germany
    J Bacteriol 184:4359-68. 2002
    ..This essential housekeeping gene is functionally independent of the integration status, due to reconstitution of its terminal codons by phage sequence...
  68. ncbi Evidence for recombination in the major capsid gene VP60 of the rabbit haemorrhagic disease virus (RHDV)
    J Abrantes
    CIBIO, Centro de Investigacao em Biodiversidade e Recursos Geneticos, Campus Agrário de Vairão, Vairao, Portugal
    Arch Virol 153:329-35. 2008
    ..Phylogenetic analyses revealed two well separated groups. Clear evidence for recombination was found for the Hartmannsdorf strain which shows different phylogenetic profiles depending on the region of the capsid examined...
  69. ncbi Peptidoglycan hydrolytic activities associated with bacteriophage virions
    Michael Moak
    Molecular Genetics and Microbiology, and Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712 1095, USA
    Mol Microbiol 51:1169-83. 2004
    ..These studies lead to a general mechanism describing how phage genomes are transported across the bacterial cell wall...
  70. ncbi Reviewing Chandipura: a vesiculovirus in human epidemics
    Soumen Basak
    Department of Chemistry and Biochemistry, Signaling Systems Laboratory, University of California, San Diego, CA 92093, USA
    Biosci Rep 27:275-98. 2007
    ..Finally, we reveal examples of protein economy in Chandipura virus life-cycle whereby each viral protein has evolved complexity to perform multiple tasks...
  71. ncbi Molecular epidemiology of enterovirus 71 over two decades in an Australian urban community
    S A Sanders
    Division of Virology, Telethon Institute for Child Health Research, Subiaco, Australia
    Arch Virol 151:1003-13. 2006
    ..The results indicate that the major lineage circulating in Sydney N.S.W. was subgenogroup C1 with a recent switch in dominance to B4 in 2000 and 2001...
  72. pmc The nucleocytoplasmic rabies virus P protein counteracts interferon signaling by inhibiting both nuclear accumulation and DNA binding of STAT1
    Aurore Vidy
    Unité Mixte de Virologie Moléculaire et Structurale, UMR 2472, CNRS, 91198 Gif sur Yvette Cedex, France
    J Virol 81:4255-63. 2007
    ..Then products of the rabies virus P gene are able to counteract IFN signaling by creating both cytoplasmic and nuclear blocks for STAT1...
  73. pmc Pseudorabies virus UL36 tegument protein physically interacts with the UL37 protein
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:3065-71. 2002
    ..Zhou, D. Chen, J. Jakana, F. J. Rixon, and W. Chiu, J. Virol. 73:3210-3218, 1999), with the UL37 protein is an important early step in tegumentation during virion morphogenesis in the cytoplasm...
  74. ncbi Reactivation of Epstein-Barr virus: regulation and function of the BZLF1 gene
    S H Speck
    Dept of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
    Trends Microbiol 5:399-405. 1997
    ..Understanding this switch may facilitate development of strategies to interfere with EBV infection...
  75. ncbi Identification and characterization of rabbit hemorrhagic disease virus genetic variants isolated in Korea
    Jae Ku Oem
    National Veterinary Research and Quarantine Service, Ministry of Agriculture, Animal Disease Diagnostic Center Division, Anayang, Republic of Korea
    J Vet Med Sci 71:1519-23. 2009
    ..Taken together, the recent RHDVa strains have gradually replaced the original RHDV and are the predominant strains in Korea...
  76. ncbi Structural properties of the human respiratory syncytial virus P protein: evidence for an elongated homotetrameric molecule that is the smallest orthologue within the family of paramyxovirus polymerase cofactors
    María T Llorente
    Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain
    Proteins 72:946-58. 2008
    ..This model will be an useful tool to make rational mutations and test the role of specific amino acids in the oligomerization and functional properties of the HRSV P protein...
  77. ncbi Intracellular delivery of large molecules and small particles by cell-penetrating proteins and peptides
    Bhawna Gupta
    Department of Pharmaceutical Sciences, Bouve College of Health Sciences, Northeastern University, Boston, MA 02115, USA
    Adv Drug Deliv Rev 57:637-51. 2005
    ....
  78. ncbi Isolation and identification of a non-haemagglutinating strain of rabbit hemorrhagic disease virus from China and sequence analysis for the VP60 Gene
    Lang Tian
    Lab of Animal Preventive Medicine and Bio engineering, Animal Science and Technology College, Sichuan Agricultural University, Ya an 625014, PR China
    Virus Genes 35:745-52. 2007
    ....
  79. ncbi Changes in VP2 gene during the attenuation of very virulent infectious bursal disease virus strain Gx isolated in China
    X M Wang
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Science, 427 Maduan Street, 150001, Harbin, P R China
    Avian Dis 48:77-83. 2004
    ..Virus neutralization tests with Gx strain showed that the antigenicities are similar from Gx to CEF-20...
  80. pmc Evolutionary patterns of eastern equine encephalitis virus in North versus South America suggest ecological differences and taxonomic revision
    Nicole C Arrigo
    Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    J Virol 84:1014-25. 2010
    ....
  81. pmc Interaction of vesicular stomatitis virus P and N proteins: identification of two overlapping domains at the N terminus of P that are involved in N0-P complex formation and encapsidation of viral genome RNA
    Mingzhou Chen
    Department of Molecular Genetics, Section of Virology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Virol 81:13478-85. 2007
    ..Taking these results together, we conclude that the N-terminal 11 to 30 aa are required for N(0)-P complex formation but the N-terminal 210 aa are required for genome RNA encapsidation...
  82. ncbi Augmentation of chemokine production by severe acute respiratory syndrome coronavirus 3a/X1 and 7a/X4 proteins through NF-kappaB activation
    Noriyuki Kanzawa
    Department of Immunotherapeutics, Tokyo Medical and Dental University, Graduate School, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    FEBS Lett 580:6807-12. 2006
    ..Our results suggest potential involvement of 3a/X1 and 7a/X4 proteins in the pathological inflammatory responses in SARS...
  83. ncbi Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995
    G Le Gall
    Unité de virologie, Immunologie, Parasitologie Aviaires et Cunicoles, Centre National d Etudes Veterinaires et Alimentaires, Zoopole, Ploufragan, France
    J Gen Virol 79:11-6. 1998
    ..The possible evolution of RHDV is discussed...
  84. pmc Structural protein requirements in equine arteritis virus assembly
    Roeland Wieringa
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 78:13019-27. 2004
    ....
  85. ncbi Characterization of pseudotype VSV possessing HCV envelope proteins
    Y Matsuura
    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Virology 286:263-75. 2001
    ..The pseudotype VSV possessing the chimeric HCV glycoproteins might offer an efficient tool for future research on cellular receptors for HCV and for the development of prophylactics and therapeutics for hepatitis C...
  86. pmc Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus
    Haitao Ding
    Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, Alabama 35294, USA
    J Virol 80:2808-14. 2006
    ..The interdimer interactions observed in the noncrystallographic packing may offer insight into the mechanism of the RNA polymerase processive reaction along the viral nucleocapsid-RNA template...
  87. pmc Identification of a novel structural protein of arteriviruses
    E J Snijder
    Department of Virology, Leiden University Medical Center, Leiden, The Netherlands
    J Virol 73:6335-45. 1999
    ..An analysis of purified EAV particles revealed that the E protein is a structural protein. By using reverse genetics, we demonstrated that both the EAV E and GS proteins are essential for the production of infectious progeny virus...
  88. pmc Severe acute respiratory syndrome coronavirus group-specific open reading frames encode nonessential functions for replication in cell cultures and mice
    Boyd Yount
    Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7435, USA
    J Virol 79:14909-22. 2005
    ....
  89. pmc Mutagenesis of the active-site cysteine in the ubiquitin-specific protease contained in large tegument protein pUL36 of pseudorabies virus impairs viral replication in vitro and neuroinvasion in vivo
    Sindy Böttcher
    Friedrich Loeffler Institut, Institute of Molecular Biology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Virol 82:6009-16. 2008
    ..Thus, the DUB activity is important for PrV replication in vitro and for neuroinvasion in mice...
  90. ncbi Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins
    Yu Kawasaki
    RIKEN Discovery Research Institute, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Gen Virol 85:3575-83. 2004
    ..To investigate the spatial relationships between IE1 and viral structural proteins in infected cells, three GFP-tagged viral components were expressed together with DsRed-tagged IE1...
  91. ncbi Molecular epidemiology of hepatitis A virus infections in Catalonia, Spain, 2005-2009: circulation of newly emerging strains
    Unai Perez-Sautu
    Enteric Virus Laboratory, Department of Microbiology and Institute of Nutrition and Food Safety, University of Barcelona, Barcelona, Spain
    J Clin Virol 52:98-102. 2011
    ..In spite of annual vaccination campaigns, hepatitis A cases increased in Catalonia (North-East Spain) in the period 2002-2005 calling for the elucidation of the underlying mechanisms associated to the epidemiological shifts...
  92. ncbi Phylogenetic relationships in the Lymphocryptovirus genus of the Gammaherpesvirinae
    Clive S Gerner
    Medical Research Council Virology Unit, Institute of Virology, Church Street, G11 5JR, Glasgow, UK
    Virus Res 99:187-92. 2004
    ..In particular, it was inferred that separation of the EBV lineage from that of the gorilla virus plus the chimpanzee/orangutan virus may have predated separation of the present day host species...
  93. ncbi Development and large-scale use of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens
    Jacob Pitcovski
    MIGAL, South Industrial Area, P O Box 831, Kiryat Shmona 11016, Israel
    Vaccine 21:4736-43. 2003
    ..Over 250 million birds have already been vaccinated with this vaccine. The advantages of a subunit vaccine over an inactivated one are discussed. This approach will enable rapid adjustment to new virulent strains if and when they appear...
  94. ncbi Purification of the IDIR strain of group B rotavirus and identification of viral structural proteins
    S L Vonderfecht
    Upjohn Company, Kalamazoo, Michigan 49001
    Virology 194:277-83. 1993
    ....
  95. ncbi Phylogenetic analysis of rabbit haemorrhagic disease virus (RHDV) strains isolated between 1988 and 2003 in eastern Hungary
    K Matiz
    Central Veterinary Institute, Institute of Debrecen, Debrecen, Hungary
    Arch Virol 151:1659-66. 2006
    ..These findings were supported by the alignments of the deduced amino acid sequences of the VP60 gene and strongly suggest the presence of the RHDVa subtype in Hungary...
  96. pmc Virion-wide protein interactions of Kaposi's sarcoma-associated herpesvirus
    Ramona Rozen
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Virol 82:4742-50. 2008
    ....
  97. ncbi Nucleotide sequences of genome segments 6 and 7 of Bombyx mori cypovirus 1, encoding the viral structural proteins V4 and V5, respectively
    K Hagiwara
    Venture Laboratory, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Gen Virol 81:1143-7. 2000
    ..56 kDa in BmN4 cells infected with BmCPV-1, reacted with a cluster of four viral structural proteins ranging from ca. 34 to 40 kDa (V5)...
  98. ncbi Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity
    D J Jackwood
    Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA
    Virology 377:110-6. 2008
    ..Together, the molecular and pathogenicity data indicate that a single amino acid mutation from Histidine (H) to Glutamine (Q) or Asparagine (N) at position 253 in VP2 will markedly increase the virulence of an attenuated IBDV...
  99. ncbi A comparison of the VP1, VP2, and VP4 regions for molecular typing of human enteroviruses
    David Perera
    Institute of Health and Community Medicine, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia
    J Med Virol 82:649-57. 2010
    ..These options would enhance the capability of laboratories in identifying these viruses and would greatly help in outbreaks of hand, foot, and mouth disease...
  100. ncbi Large-scale manufacture and use of recombinant VP2 vaccine against infectious bursal disease in chickens
    Jun Rong
    College of Life Science Yangtze University, Jingzhou, Hubei 434025, PR China
    Vaccine 25:7900-8. 2007
    ..Correlation coefficient between the OD values of VP2-based ELISA and the virions-based ELISA was 0.782. The results will make it possible for the vaccine to be produced commercially and used in poultry industry in large scale...
  101. ncbi Formation of subviral particles of the capsid protein VP2 of infectious bursal disease virus and its application in serological diagnosis
    Sohini Dey
    Recombinant DNA Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India
    J Virol Methods 157:84-9. 2009
    ..The assay proved to be sensitive, specific and accurate as compared to the serum neutralization test and agar gel immunodiffusion test. The recombinant VP2 antigen is a suitable alternative to whole viral antigen...

Research Grants64

  1. CTL response to AAV Vector
    Richard J Samulski; Fiscal Year: 2013
    ....
  2. Molecular Biology of Eukaryotic Viruses
    Bert L Semler; Fiscal Year: 2012
    ..transcription, RNA processing, viral translation and protein processing, assembly and transport of viral structural proteins, and the structures of virus particles...
  3. Theiler?s virus as a potential cause of Vilyuisk encephalitis
    HOWARD LEE LIPTON; Fiscal Year: 2010
    ..The murine counterpart, Theiler's murine encephalomyelitis virus, provides a highly relevant experimental animal model for multiple sclerosis. ..
  4. Viral Gastroenteritis: Basis of Protection and Virulence
    HARRY BERNARD GREENBERG; Fiscal Year: 2013
    ..They also represent a highly tractable system to study basic aspects of innate, acquired and mucosal immunity and viral pathogenesis, all important issues for the future of infectious disease prevention and treatment. ..
  5. Newcastle Disease Virus Vectored Vaccines for Norovirus Infection
    Siba K Samal; Fiscal Year: 2013
    ..We expect that this study will allow us to evaluate whether live NDV vectored vaccines and purified VLPs expressed by NDVs can be a novel strategy in providing immunity and protection against human norovirus infection. ..
  6. Rotavirus: Studies of Intestinal Tropism, Viral Entry, and Rescue
    HARRY BERNARD GREENBERG; Fiscal Year: 2013
    ..It is our hypothesis that we can accomplish this aim by utilizing approaches recently validated for reovirus and orbiviruses. ..
  7. Mechanisms in Polyoma and Papillomavirus Assembly
    ROBERT GARCEA; Fiscal Year: 2013
    ..with regard to its regulation within virus-infected cells and the specific biochemical properties of the viral structural proteins that determine the pathways of assembly...
  8. Control of Viral Pathogenesis by Regulation of 2-5A Levels
    Susan R Weiss; Fiscal Year: 2013
    ....
  9. Next Generation of Recombinant AAV Serotype Vectors for Gene Therapy
    Sergei Zolotukhin; Fiscal Year: 2013
    ....
  10. STRUCTURE AND ASSEMBLY OF VIRUSES
    STEPHEN COPLAN HARRISON; Fiscal Year: 2012
    ..abstract_text> ..
  11. Regulation of Rotavirus Replication
    MARY KOLB ESTES; Fiscal Year: 2013
    ..The specific aims of our proposed work are (1) To determine how rotavirus NSP4 affects calcium homeostasis, and (2) To dissect NSP4 regulation of viral morphogenesis. ..
  12. The plant-like vacuole of Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2013
    ..We think that the PLV plays a central role during the extracellular phase of the parasite not only in resisting environmental stress but also as it prepares itself for invading the next host cell. ..
  13. Viral and Cellular Determinants of HCV Assembly
    GUANGXIANG GEORGE LUO; Fiscal Year: 2013
    ..We have also found that HCV NS proteins interact with viral structural proteins, as demonstrated by experiments with the mammalian two-hybrid system and co-immunoprecipitation assay...
  14. Cellular attachment, penetration and transport of non-enveloped dsRNA viruses
    Z Hong Zhou; Fiscal Year: 2013
    ..These mechanisms and structures will be correlated with those of other enveloped and non-enveloped viruses, including the ones cited above. ..
  15. A novel vacuolar compartment in Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2010
    ..This work is designed to test the hypothesis that a multivesicular endosome present in these parasites is involved in trafficking of proteins to acidocalcisomes, and has a role in osmoregulation and intracellular pH homeostasis. ..
  16. Epitope shifting and Antibody Maturation during Rotavirus Infection
    Benjamin W Spiller; Fiscal Year: 2012
    ..We will explore and determine the molecular basis for epitope shift. ..
  17. rAAV vaccine vector
    Selvarangan Ponnazhagan; Fiscal Year: 2012
    ..of effective cancer vaccines include induction of sustained, high-level transgene expression, absence of viral structural proteins, non-pathogenicity, and absence of toxicity...
  18. Virus-Like Particle Vaccines for Pandemic Influenza
    Ted M Ross; Fiscal Year: 2010
    ..These VLPs are comprised of viral structural proteins that assemble spontaneously cells...
  19. Alpha-Herpesvirus Transport on Axons
    Gregory Allan Smith; Fiscal Year: 2013
    ..This proposal focuses on understanding the cellular mechanisms used by herpesviruses to spread within the nervous system and cause disease, with the long term goal of developing new treatments to intervene with disease progression. ..
  20. E3 Ubiquitin Ligase Functions in Enveloped Virus Budding
    ANDREW PAUL NORGAN; Fiscal Year: 2012
    ..We will systematically address the role of ubiquitin ligase interactions on the budding ofthe viral structural proteins HIV Gag and EBOV VP40, using the yeast system...
  21. Alpha-herpevirus assembly egress and viral particle heterogeneity
    Gregory Allan Smith; Fiscal Year: 2013
    ..This proposal focuses on understanding the molecular mechanisms that underlie the assembly and egress of herpesvirus particles, with the long- term goal of identifying new targets for the intervention of disease progression. ..
  22. Rotavirus VP6 Specific Human IgA Antibodies
    James E Crowe; Fiscal Year: 2010
    ..If we show that this method of killing virus does inhibit rotavirus, we will understand how rotavirus antibodies and vaccines work and we can use this knowledge to create vaccines that work in a similar manner against other pathogens. ..
  23. Structural controls of functional receptor and antibody binding to viral capsids
    Colin R Parrish; Fiscal Year: 2013
    ..structure over their life cycles, the mechanisms of binding to different receptors, how differences in viral structural proteins can control their host ranges, the processes of cell infection, and how antibody binding can neutralize ..
  24. Alpha-herpesvirus assembly, egress and viral particle heterogeneity
    Gregory Smith; Fiscal Year: 2009
    ..abstract_text> ..
  25. STRUCTURE AND ASSEMBLY OF VIRUSES
    STEPHEN COPLAN HARRISON; Fiscal Year: 2013
    ..The properties of these particles, in particular the ease of generating particles with a mutated envelope protein, will allow us to analyze steps in fusion that have not previously been accessible to single-particle kinetic analysis. ..
  26. Host determinants of alphavirus replication
    Richard W Hardy; Fiscal Year: 2013
    ..Co-expression in Drosophila of the replicon and viral structural proteins leads to the production of replicon containing particles capable of a single round of infection...
  27. Alpha-Herpevirus Transport in Axons
    Gregory Smith; Fiscal Year: 2009
    ..abstract_text> ..
  28. ENHANCING THE IMMUNOGENICITY OF HUMAN ROTAVIRUS VACCINES
    Linda Saif; Fiscal Year: 2006
    ..abstract_text> ..
  29. Simian Virus 40 (SV40) uncoating and penetration
    Daniel Hebert; Fiscal Year: 2009
    ..Furthermore, understanding the viral-mediated DNA delivery process also has potential applications for increasing the efficiency of DNA delivery vehicles for gene therapy. ..
  30. VIRAL PROTEINS IN ROTAVIRUS REPLICATION
    John Patton; Fiscal Year: 1991
    ..These results should provide valuable information on events in the replication of the rotaviruses that may be useful for developing strategies of controlling diseases caused by these viruses...
  31. VIRAL GASTROENTERITIS--BASIS OF PROTECTION AND VIRULENCE
    Harry Greenberg; Fiscal Year: 2003
    ..In order to better understand the molecular and virologic basis of virulence and/or host tropism, we will identify relevant steps in the viral replication cycle in vivo that are restricted on the basis of host range. ..
  32. SURFACE MEMBRANES IN NORMAL AND CANCER CELLS
    Paul Atkinson; Fiscal Year: 1991
    ..Detailed analysis of processing of the rotavirus VP7 has implicated a sub-compartment of the ER in animal cells in which this glycoprotein is processed and we plan to further characterize this compartment...
  33. Masking the GH-loop: model for human Rhinovirus vaccine
    Peter Nara; Fiscal Year: 2001
    ..In addition, vaccines against a wide variety of viruses, bacteria and parasites in both humans and animals for which no vaccines exist to date could be made using this approach. ..
  34. DEVELOPMENT OF ROTAVIRUS DNA VACCINES
    John Herrmann; Fiscal Year: 2003
    ..Immunization with DNA vaccines that express specific rotaviral proteins offers a new approach to vaccination against rotaviruses and may provide the next generation of rotavirus vaccines. ..
  35. IMMUNE RESPONSES TO ROTAVIRUS-- ROLE MATERNAL ANTIBODIES
    Linda Saif; Fiscal Year: 2003
    ..Clarification of the mechanisms of suppression by maternal Ab and means to overcome them will lead to more efficacious vaccines for both RV and other pathogens infecting neonates. ..
  36. Enhancing Gene Therapy by Designing Chimeric AAV Virions
    JARROD JOHNSON; Fiscal Year: 2009
    ..Through manipulating these components, it will soon be possible to design virions that are specialized for delivering therapeutics to cells affected by many different genetic disorders and diseases. ..
  37. PARVOVIRUS STRUCTURE, CAPSID ASSEMBLY, AND RECEPTORS
    Colin Parrish; Fiscal Year: 2007
    ..abstract_text> ..
  38. STRUCTURE, SYNTHESIS AND ASSEMBLY OF PICORNAVIRUSES
    ROLAND RUECKERT; Fiscal Year: 1991
    ..Selected mutants will be analyzed by x-ray crystallography at Purdue University to determine the effect of the mutation on the 3-dimensional structure of the pocket...
  39. Primate enteroviruses transmission in Bangladesh
    Lisa Jones Engel; Fiscal Year: 2007
    ..Ultimately, this data will provide insight into the potential emergence of NHP-borne pathogens and help to deter the spread of emerging zoonotic threats. [unreadable] [unreadable]..
  40. DETERMINANTS OF PARVOVIRUS ASSEMBLY AND INFECTIVITY
    IAN MAXWELL; Fiscal Year: 2000
    ..These studies will elucidate structural determinants required for assembly and/or infectivity of parvovirus virions. ..
  41. INCIDENCE & IDENTITY OF THE RA-1 PARVOVIRUS
    Robert Simpson; Fiscal Year: 1990
    ..Synthetic polypeptides mimicking the putative viral structural proteins will be made and tested for their ability to elicit antibodies that confer protective immunity in ..
  42. POLIOVIRUS USING INFECTIOUS VIRAL CDNA CLONE
    VINCENT RACANIELLO; Fiscal Year: 2002
    ..The results of these experiments will contribute to the long term goal of this research, which is to provide a complete description of the replication and pathogenesis of a human viral pathogen. ..
  43. Characterization of cougar lentiviruses
    Mary Poss; Fiscal Year: 2003
    ..5%/decade, and there is selection against change of viral structural proteins. To further advance this important model, we will initiate studies to discern potential viral mechanisms ..
  44. Hyperacetylation of Biologically Active SV40 Chromatin
    Barry Milavetz; Fiscal Year: 2006
    ..Antibodies will be used which recognize hyperacetylated histones, methylated histones, and viral structural proteins, all of which we have shown to be associated with SV40 chromosomes during this time period in ..
  45. T reg Cells in Myocarditis
    Sally A Huber; Fiscal Year: 2010
    ..Determine whether the T reg cells are antigen specific (virus), cardiovascular specific (autoimmunity), or non-antigen specific. ..
  46. ASSEMBLY AND MORPHOGENESIS OF DNA TUMOR VIRUSES
    Harumi Kasamatsu; Fiscal Year: 2006
    ..with cellular proteins and virally encoded early-gene products, specific structural motifs of SV40 viral structural proteins (VP1, VP2/3) are beleived to direct each step of the subvinon and vinion assembly processes and virus ..
  47. Development of an Intranasal Rotavirus Subunit Vaccine
    ALEXANDER ANDRIANOV; Fiscal Year: 2002
    ..In Phase III SBIR studies, we will conduct safety, immunogencity and efficacy trials in humans and seek FDA approval. ..
  48. Cellular Immunity to BK Virus
    Simon Lacey; Fiscal Year: 2005
    ..Such information may be useful in guiding clinical management, and lay the foundation for possible future vaccines. ..
  49. EXPRESSION OF FUNCTIONAL POLIOVIRUS REPLICATION PROTEINS
    CASEY MORROW; Fiscal Year: 2002
    ....
  50. ROTAVIRUS VP3--MOLECULAR DETERMINANTS OF FUNCTION
    Suzanne Matsui; Fiscal Year: 1991
    ....
  51. Role of JCV coat proteins in neuropathogenesis of PML
    MEGAN GASPAROVIC; Fiscal Year: 2007
    ..abstract_text> ..
  52. INFLUENZA VIRUS ASSEMBLY
    Yoshihiro Kawaoka; Fiscal Year: 2009
    ..The virion incorporation of vRNAs appears to involve viral structural proteins that sequester the viral genome segments into budding particles...
  53. STUDIES IN POLYOMA TRANSFORMED CELLS--VIRION PROTEINS
    RICHARD CONSIGLI; Fiscal Year: 1993
    ..Studies will also include the identification of possible normal cell polypeptide growth factor(s) which share the polyoma-mouse cell receptor. ..
  54. ROTAVIRUS VP5 PERMEABILIZES MEMBRANES
    ERICH MACKOW; Fiscal Year: 2002
    ..These studies address an essential step in the rotavirus entry process and basic mechanisms by which non-enveloped viral proteins permeabilize cellular membranes during entry. ..
  55. STRUCTURAL BASIS FOR ROTAVIRUS ENTRY INTO CELLS
    PHILIP DORMITZER; Fiscal Year: 2000
    ..My long term career goal is to apply basic scientific techniques to clinically important virologic problems. ..
  56. REPLICATION OF NEGATIVE STRAND RNA VIRUSES
    Judith Lesnaw; Fiscal Year: 1993
    ..The virion consits of a central ribonucleoprotein core surrounded by a membrane, and contains five viral structural proteins: L ( a polymerase subunit) 2,109 aa; G (the surface glycoprotein) 511 aa; M (the matrix protein) 229 aa; ..
  57. Agent-based modeling of virus-host cell interaction in salivary gland disease
    SUZY VASA; Fiscal Year: 2009
    ..In sum, I hope to advance research into the cause of SGD by developing a computational model that can be used to examine theories about interacting virus-host proteins and molecules. ..
  58. Amyloid Precursor Protein Signaling
    Mark Bothwell; Fiscal Year: 2006
    ..Effects of agonistic APP monoclonal antibody, beta-amyloid peptide, LRP, and alpha-2 macroglobulin on APP signaling will be assessed. ..
  59. CHEMICAL NATURE OF LEUKEMIA ANTIGENS
    HARRY SNYDER; Fiscal Year: 1980
    ..The nature of cell surface molecules reactive with antisera to individual viral structural proteins will be determined. The reactivity of feline sera with such cell surface proteins will also be analyzed...
  60. STRUCTURAL STUDIES ON ROTAVIRUSES
    BIDADI V PRASAD; Fiscal Year: 2002
    ..4) To carry out structural analysis of NSP4, an intracellular receptor, and study its interaction with VP6, and viral cell attachment protein VP4. ..
  61. PRODUCTION OF SV40-SPECIFIED ANTIGENS IN E COLI
    Geoffrey Wilson; Fiscal Year: 1980
    ..The purpose of producing distinct sections of the T and t antigens is to investigate their biological role. ..
  62. HIGH RESOLUTION CRYSTALLOGRAPHIC STUDIES OF POLIOVIRUS
    JAMES HOGLE; Fiscal Year: 2001
    ..The crystallographic analyses will be carried out in collaboration with a number other research groups in order to provide a direct interplay between structural molecular genetic studies. ..
  63. Human Antibody Responses to Rotavirus
    James Crowe; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  64. MOLECULAR PATHOGENESIS IN THE POLYOMA VIRUS/MOUSE SYSTEM
    Thomas Benjamin; Fiscal Year: 2000
    ..Finally, an attempt will be made to identify, map, and ultimately clone, genes of the host that confer susceptibility or resistance to tumor induction by the virus. ..