small ubiquitin related modifier proteins

Summary

Summary: A class of structurally related proteins of 12-20 kDa in size. They covalently modify specific proteins in a manner analogous to UBIQUITIN.

Top Publications

  1. ncbi The polycomb protein Pc2 is a SUMO E3
    Michael H Kagey
    Department of Biochemistry and Molecular Genetics, Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    Cell 113:127-37. 2003
  2. ncbi Concepts in sumoylation: a decade on
    Ruth Geiss-Friedlander
    Department of Biochemie I, Faculty of Medicine, University of Goettingen, Humboldt Allee 23, 37073 Goettingen, Germany
    Nat Rev Mol Cell Biol 8:947-56. 2007
  3. ncbi Protein modification by SUMO
    Erica S Johnson
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Annu Rev Biochem 73:355-82. 2004
  4. ncbi Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9
    M H Tatham
    Institute of Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 5ST, United Kingdom
    J Biol Chem 276:35368-74. 2001
  5. pmc Identification of a SUMO-binding motif that recognizes SUMO-modified proteins
    Jing Song
    Division of Immunology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 101:14373-8. 2004
  6. ncbi Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3
    H Saitoh
    The Picower Institute for Medical Research, Manhasset, New York 11030, USA
    J Biol Chem 275:6252-8. 2000
  7. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
  8. ncbi Role of SUMO-interacting motif in Daxx SUMO modification, subnuclear localization, and repression of sumoylated transcription factors
    Ding Yen Lin
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan, Republic of China
    Mol Cell 24:341-54. 2006
  9. ncbi RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
    Carsten Hoege
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Nature 419:135-41. 2002
  10. pmc Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteins
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    EMBO J 26:4102-12. 2007

Research Grants

Detail Information

Publications216 found, 100 shown here

  1. ncbi The polycomb protein Pc2 is a SUMO E3
    Michael H Kagey
    Department of Biochemistry and Molecular Genetics, Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    Cell 113:127-37. 2003
    ..These results demonstrate that Pc2 is a SUMO E3, and suggest that PcG bodies may be sumoylation centers...
  2. ncbi Concepts in sumoylation: a decade on
    Ruth Geiss-Friedlander
    Department of Biochemie I, Faculty of Medicine, University of Goettingen, Humboldt Allee 23, 37073 Goettingen, Germany
    Nat Rev Mol Cell Biol 8:947-56. 2007
    ..At first glance, these effects have nothing in common; however, it seems that they all result from changes in the molecular interactions of the sumoylated proteins...
  3. ncbi Protein modification by SUMO
    Erica S Johnson
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Annu Rev Biochem 73:355-82. 2004
    ..This review discusses our current understanding of how SUMO conjugation is controlled, as well as the roles of SUMO in a number of biological processes...
  4. ncbi Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9
    M H Tatham
    Institute of Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 5ST, United Kingdom
    J Biol Chem 276:35368-74. 2001
    ..The ability to form polymeric chains is not shared by SUMO-1, and although all SUMO species use the same conjugation machinery, modification by SUMO-1 and SUMO-2/-3 may have distinct functional consequences...
  5. pmc Identification of a SUMO-binding motif that recognizes SUMO-modified proteins
    Jing Song
    Division of Immunology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 101:14373-8. 2004
    ..The SUMO-binding motif exists in nearly all proteins known to be involved in SUMO-dependent processes, suggesting its general role in sumoylation-dependent cellular functions...
  6. ncbi Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3
    H Saitoh
    The Picower Institute for Medical Research, Manhasset, New York 11030, USA
    J Biol Chem 275:6252-8. 2000
    ..Together, these results support the concept of important distinctions between the SUMO-2/3 and SUMO-1 conjugation pathways and suggest a role for SUMO-2/3 in the cellular responses to environmental stress...
  7. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
    ..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
  8. ncbi Role of SUMO-interacting motif in Daxx SUMO modification, subnuclear localization, and repression of sumoylated transcription factors
    Ding Yen Lin
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan, Republic of China
    Mol Cell 24:341-54. 2006
    ..Our results provide mechanistic insights into Daxx in SUMO-dependent transcriptional control and subnuclear compartmentalization...
  9. ncbi RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
    Carsten Hoege
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Nature 419:135-41. 2002
    ..We demonstrate that these modifications differentially affect resistance to DNA damage, and that damage-induced PCNA ubiquitination is elementary for DNA repair and occurs at the same conserved residue in yeast and humans...
  10. pmc Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteins
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    EMBO J 26:4102-12. 2007
    ..Hence, we describe a family of SIM-containing RING-finger proteins that potentially regulates eukaryotic genome stability through linking SUMO-interaction with ubiquitin conjugation...
  11. ncbi Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast
    E Mossessova
    Biochemistry Department, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell 5:865-76. 2000
    ..Genetic analysis guided by the structure further reveals a regulatory element N-terminal to the proteolytic domain that is required for cell growth in yeast...
  12. pmc The Slx5-Slx8 complex affects sumoylation of DNA repair proteins and negatively regulates recombination
    Rebecca C Burgess
    Department of Biological Sciences, Columbia University, and Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    Mol Cell Biol 27:6153-62. 2007
    ..In addition, the complex inhibits Rad51-independent recombination via modulating the sumoylation of DNA repair proteins...
  13. ncbi A new protease required for cell-cycle progression in yeast
    S J Li
    University of Chicago, Department of Biochemistry and Molecular Biology, Illinois 60637, USA
    Nature 398:246-51. 1999
    ..The pattern of Smt3-coupled proteins in yeast changes markedly throughout the cell cycle, and specific conjugates accumulate in ulp1 mutants. Ulp1 has several functions, including an essential role in the G2/M phase of the cell cycle...
  14. pmc SUMO junction-what's your function? New insights through SUMO-interacting motifs
    Oliver Kerscher
    Biology Department, Millington Hall, Room 328, Landrum Drive, College of William and Mary, Williamsburg, Virginia 23185, USA
    EMBO Rep 8:550-5. 2007
    ....
  15. ncbi A SIM-ultaneous role for SUMO and ubiquitin
    J Jefferson P Perry
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Trends Biochem Sci 33:201-8. 2008
    ..The human STUbL, RNF4, is implicated in several diseases including cancer, highlighting the importance of characterizing the cellular functions of STUbLs...
  16. ncbi Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p
    E S Johnson
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 272:26799-802. 1997
    ..cerevisiae. These results suggest that, like ubiquitination, Smt3p conjugation may be a critical modification in cell cycle regulation...
  17. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
    ..Moreover, PIASy binds to nuclear matrix-associated DNA sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated subnuclear sequestration accounts for the repression of LEF1 activity...
  18. pmc The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein
    S J Li
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 20:2367-77. 2000
    ..Our data suggest the existence of a feedback mechanism that limits Smt3-protein ligation when Smt3 deconjugation by both Ulp1 and Ulp2 is compromised, allowing a partial recovery of cell function...
  19. ncbi Sumoylation of the estrogen receptor alpha hinge region regulates its transcriptional activity
    Stephanie Sentis
    Institut National de la Sante et de la Recherche Medicale, Unité 590, Centre Leon Berard, 28 rue Laennec, 69373 Lyon Cedex 08, France
    Mol Endocrinol 19:2671-84. 2005
    ..These findings identify sumoylation as a new mechanism modulating ERalpha-dependent cellular response and provide a link between the SUMO and estrogen pathways...
  20. ncbi Global analysis of protein sumoylation in Saccharomyces cerevisiae
    James A Wohlschlegel
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:45662-8. 2004
    ..Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes...
  21. ncbi Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics
    Alfred C O Vertegaal
    Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Mol Cell Proteomics 5:2298-310. 2006
    ....
  22. ncbi Fission yeast Rnf4 homologs are required for DNA repair
    Ana Kosoy
    Department of Oncological Sciences, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Biol Chem 282:20388-94. 2007
    ..pombe Ulp2/Smt4 homolog that, when overexpressed, reduced SUMO levels and suppressed the DNA damage sensitivity of rfp1Delta rfp2Delta cells...
  23. ncbi Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad4
    Che Chang Chang
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China
    J Biol Chem 280:10164-73. 2005
    ..Together, these results indicate that Daxx suppresses Smad4-mediated transcriptional activity by direct interaction with the sumoylated Smad4 and identify a novel role of Daxx in regulating transforming growth factor beta signaling...
  24. pmc Novel proteomics strategy brings insight into the prevalence of SUMO-2 target sites
    Henri A Blomster
    Department of Biology, Abo Akademi University and University of Turku, FI 20521 Turku, Finland
    Mol Cell Proteomics 8:1382-90. 2009
    ..Given that different types of motifs are bound by Ubc9 using alternative mechanisms, our data suggest that the preference of SUMO-2 targeting mechanism depends on the biological function of the substrate...
  25. ncbi Regulation of DNA repair throughout the cell cycle
    Dana Branzei
    FIRC Institute of Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy
    Nat Rev Mol Cell Biol 9:297-308. 2008
    ..Recent studies have provided insights into the mechanisms that contribute to DNA repair in specific cell-cycle phases and have highlighted the mechanisms that ensure cell-cycle progression or arrest in normal and cancerous cells...
  26. pmc Emerging extranuclear roles of protein SUMOylation in neuronal function and dysfunction
    Stephane Martin
    MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    Nat Rev Neurosci 8:948-59. 2007
    ....
  27. pmc Activation of the Slx5-Slx8 ubiquitin ligase by poly-small ubiquitin-like modifier conjugates
    Janet R Mullen
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
    J Biol Chem 283:19912-21. 2008
    ..Slx5-Slx8 primarily mono-ubiquitinated the N-terminal SUMO moiety of the chain. These data indicate that the Slx5-Slx8 Ub ligase is stimulated by poly-SUMO conjugates and that it can ubiquitinate a poly-SUMO chain...
  28. pmc Genetic and proteomic evidence for roles of Drosophila SUMO in cell cycle control, Ras signaling, and early pattern formation
    Minghua Nie
    Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 4:e5905. 2009
    ..In addition, our database of sumoylated proteins provides a valuable resource for those studying the roles of SUMO in development...
  29. ncbi Expression of SUMO-2/3 induced senescence through p53- and pRB-mediated pathways
    Tianwei Li
    Laboratory of Biochemistry and Proteomics Core Facility, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:36221-7. 2006
    ..Together, our results reveal that p53 and pRB can be sumoylated by SUMO-2/3 in vivo, and such modification of p53 and pRB may play roles in premature senescence and stress response...
  30. pmc The structure of SENP1-SUMO-2 complex suggests a structural basis for discrimination between SUMO paralogues during processing
    Lin Nan Shen
    Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Scotland KY16 9ST, UK
    Biochem J 397:279-88. 2006
    ....
  31. ncbi SUMO association with repressor complexes, emerging routes for transcriptional control
    Mario Garcia-Dominguez
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Consejo Superior de Investigaciones Cientificas CSIC, Av Americo Vespucio, 41092 Seville, Spain
    Biochim Biophys Acta 1789:451-9. 2009
    ....
  32. ncbi A mechanism for inhibiting the SUMO pathway
    Roberto Boggio
    European Institute of Oncology, Department of Experimental Oncology, 20141 Milan, Italy
    Mol Cell 16:549-61. 2004
    ..They also point out once again to the extraordinary ability of eukaryotic viruses to interfere with the biology of host cells by targeting fundamental biochemical processes...
  33. ncbi The protein stability and transcriptional activity of p63alpha are regulated by SUMO-1 conjugation
    Pamela Ghioni
    Department of Biomolecular and Biotechnological Sciences, University of Milan, Milan, Italy
    Cell Cycle 4:183-90. 2005
    ..The findings that DeltaNp63alpha protein levels are regulated by SUMO-1 and that this regulation is altered in natural p63 mutants, suggest that SUMO conjugation to p63 plays a critical role in regulating the biological activity of p63...
  34. ncbi Rad52 sumoylation and its involvement in the efficient induction of homologous recombination
    Takashi Ohuchi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    DNA Repair (Amst) 7:879-89. 2008
    ....
  35. pmc SUMO-2/3 regulates topoisomerase II in mitosis
    Yoshiaki Azuma
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
    J Cell Biol 163:477-87. 2003
    ..Together, our results suggest that SUMO conjugation is important for chromosome segregation in metazoan systems, and that mobilization of topoisomerase II from mitotic chromatin may be a key target of this modification...
  36. pmc SUMOylation of the MAGUK protein CASK regulates dendritic spinogenesis
    Hsu Wen Chao
    Graduate Institute of Life Science, National Defense Medical Center, National Yang Ming University, Taipei 11529, Taiwan
    J Cell Biol 182:141-55. 2008
    ..1. Overexpression of a CASK-SUMO1 fusion construct, which mimicks CASK SUMOylation, impairs spine formation. Our study suggests that CASK contributes to spinogenesis and that this is controlled by SUMOylation...
  37. pmc Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis
    Tianwei Li
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8012, USA
    Proc Natl Acad Sci U S A 101:8551-6. 2004
    ..In view of the facts that hnRNPs have been implicated in RNA splicing, transport, stability, and translation, our findings suggest that sumoylation could play an important role in regulating mRNA metabolism...
  38. ncbi Transcriptional activity of peroxisome proliferator-activated receptor gamma is modulated by SUMO-1 modification
    Takayuki Ohshima
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    J Biol Chem 279:29551-7. 2004
    ..These results suggest that the PPARgamma-dependent transactivation pathway seems to be modulated by SUMO-1 modification and may serve as a novel target for apoptosis-induction therapy in cancer cells...
  39. pmc Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation
    Ben Montpetit
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    J Cell Biol 174:653-63. 2006
    ..These data suggest that sumoylation of Ndc10 and other kinetochore proteins play a critical role during the mitotic process...
  40. pmc Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase
    Shigeki Nagai
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Science 322:597-602. 2008
    ..This suggests that strand breaks are shunted to nuclear pores for a repair pathway controlled by a conserved SUMO-dependent E3 ligase...
  41. pmc SIZ1/SIZ2 control of chromosome transmission fidelity is mediated by the sumoylation of topoisomerase II
    Yoshimitsu Takahashi
    National Institutes of Health, National Institute of Child Health and Human Development, Laboratory of Gene Regulation and Development, Bethesda, Maryland 20892, USA
    Genetics 172:783-94. 2006
    ..We propose a model for the positive control of the centromeric pool of Top2p, required for high segregation fidelity, by Smt3p modification...
  42. ncbi SUMO: ligases, isopeptidases and nuclear pores
    Frauke Melchior
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Trends Biochem Sci 28:612-8. 2003
    ..In addition, studies in yeast suggest a requirement of SUMO conjugation for nuclear protein import, and specific SUMO targets depend on modification for nuclear import or export...
  43. ncbi A calcium-regulated MEF2 sumoylation switch controls postsynaptic differentiation
    Aryaman Shalizi
    Department of Pathology, Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA
    Science 311:1012-7. 2006
    ..Our findings define a mechanism underlying postsynaptic differentiation that may modulate activity-dependent synapse development and plasticity in the brain...
  44. pmc Enzymes of the SUMO modification pathway localize to filaments of the nuclear pore complex
    Hong Zhang
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Mol Cell Biol 22:6498-508. 2002
    ..Our results indicate that both SUMO modification and demodification of proteins may occur at the NPC and suggest a connection between the SUMO modification pathway and nucleocytoplasmic transport...
  45. pmc Preparation of SUMO proteases and kinetic analysis using endogenous substrates
    David Reverter
    Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, Barcelona, Spain
    Methods Mol Biol 497:225-39. 2009
    ..We will also describe methods for kinetic analysis of SUMO deconjugation and maturation using endogenous substrates...
  46. pmc Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae
    Zheng Wang
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genetics 172:1499-509. 2006
    ....
  47. ncbi PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor and repress androgen receptor-dependent transcription
    Tamotsu Nishida
    Division of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Japan
    J Biol Chem 277:41311-7. 2002
    ..Thus, PIAS1 and PIASxalpha modulate the AR-dependent transactivation, which, at least in part, can be attributed to their SUMO-E3 activity toward AR...
  48. pmc Positive and negative regulation of APP amyloidogenesis by sumoylation
    Yonghong Li
    Scios, Inc, 820 West Maude Avenue, Sunnyvale, CA 94085, USA
    Proc Natl Acad Sci U S A 100:259-64. 2003
    ..These findings provide a regulatory mechanism in APP amyloidogenesis and suggest that components in the sumoylation pathway may be critical in AD onset or progression...
  49. ncbi Functional characterization of TIP60 sumoylation in UV-irradiated DNA damage response
    Z Cheng
    Cellular Dynamics, Hefei National Laboratory, Hefei, China
    Oncogene 27:931-41. 2008
    ..Thus, this study shed new light on the function and regulation of TIP60 activity in UV-irradiated DNA damage response...
  50. ncbi Assembly of a polymeric chain of SUMO1 on human topoisomerase I in vitro
    Meiluen Yang
    Institute of Biochemistry and Molecular Biology, School of Life Science, National Yang Ming University, Taipei 112, Taiwan
    J Biol Chem 281:8264-74. 2006
    ..These results offer new insight into hTOP1 polysumoylation in response to TOP1-mediated DNA damage and may have general implications in protein polysumoylation...
  51. pmc Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1
    Zheng Xu
    Department of Biochemistry, Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong
    Biochem J 386:325-30. 2005
    ..Taken together, we suggest that the observed differential maturation process has its physiological significance in the regulation of the sumoylation pathway...
  52. pmc A novel method for high accuracy sumoylation site prediction from protein sequences
    Jialin Xu
    The Key Laboratory of Bioinformatics, Ministry of Education, China, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China
    BMC Bioinformatics 9:8. 2008
    ..In this regard, computational approaches might represent a promising method to direct experimental identification of sumoylation sites and shed light on the understanding of the reaction mechanism...
  53. pmc PDSM, a motif for phosphorylation-dependent SUMO modification
    Ville Hietakangas
    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, FI 20521, Turku, Finland
    Proc Natl Acad Sci U S A 103:45-50. 2006
    ..As the first recurrent sumoylation determinant beyond the consensus tetrapeptide, the PDSM provides a valuable tool in predicting new SUMO substrates...
  54. ncbi Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli
    Yasuhiro Uchimura
    Department of Regeneration Medicine, Institute of Molecular Embryology Genetics, Kumamoto University, Kumamoto, 860 0811, Japan
    Anal Biochem 331:204-6. 2004
  55. ncbi SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins
    Michael P Malakhov
    LifeSensors, Inc, 271 Great Valley Parkway, Malvern, PA 19355, USA
    J Struct Funct Genomics 5:75-86. 2004
    ....
  56. ncbi The fast-growing business of SUMO chains
    Helle D Ulrich
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, UK
    Mol Cell 32:301-5. 2008
    ..Like ubiquitin itself, the small ubiquitin-related modifier SUMO can form polymeric chains on many of its targets. Recent analyses have provided evidence for a number of distinct biological functions of the poly-SUMO signal...
  57. ncbi Stabilization of PML nuclear localization by conjugation and oligomerization of SUMO-3
    Chuanhai Fu
    School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
    Oncogene 24:5401-13. 2005
    ..Taken together, our studies provide first line of evidence showing that SUMO-3 is essential for PML localization and offer novel insight into the pathobiochemistry of APL...
  58. ncbi The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice
    Karim Nacerddine
    Unité d Organisation Nucléaire et Oncogénèse, INSERM U 579, Institut Pasteur, 75724 Paris Cedex 15, France
    Dev Cell 9:769-79. 2005
    ..Together, these findings reveal a major role for Ubc9, and, by implication, for the SUMO pathway, in nuclear architecture and function, chromosome segregation, and embryonic viability in mammals...
  59. ncbi SUMO-specific proteases: a twist in the tail
    Ronald Thomas Hay
    Sir James Black Centre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Trends Cell Biol 17:370-6. 2007
    ..Processing of SUMO precursors is mediated by SUMO-specific proteases that also remove SUMO from modified proteins and depolymerise poly-SUMO chains...
  60. pmc SUMO modification of a novel MAR-binding protein, SATB2, modulates immunoglobulin mu gene expression
    Gergana Dobreva
    Gene Center and Institute of Biochemistry, University of Munich, Munich 81377, Germany
    Genes Dev 17:3048-61. 2003
    ..Sumoylation is also involved in targeting SATB2 to the nuclear periphery, raising the possibility that this reversible modification of a MAR-binding protein may contribute to the modulation of subnuclear DNA localization...
  61. ncbi Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1
    J M Desterro
    School of Biomedical Science, University of St Andrews, St Andrews, Fife KY169ST Scotland
    J Biol Chem 274:10618-24. 1999
    ..As SAE1/SAE2, Ubch9, SUMO-1, and IkappaBalpha are all homogeneous, recombinant proteins, it appears that SUMO-1 conjugation of IkappaBalpha in vitro does not require the equivalent of an E3 ubiquitin protein ligase activity...
  62. ncbi Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation
    Fang Wei
    Department of Animal Biology, Mari Lowe Center for Comparative Oncology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:21551-60. 2007
    ..Therefore, sumoylation of Oct4 results in increased stability, DNA binding, and transactivation and provides an important mechanism to regulate Oct4 activity...
  63. pmc Role of SUMO in the dynamics of telomere maintenance in fission yeast
    Blerta Xhemalce
    Unite de la Dynamique du Génome, Unité Organisation Nucléaire et Oncogénèse, INSERM U579, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 104:893-8. 2007
    ..Instead, sumoylation increases telomerase activity, therefore suggesting that this modification controls the activity of a positive or negative regulator of telomerase...
  64. pmc SIRT1 sumoylation regulates its deacetylase activity and cellular response to genotoxic stress
    Yonghua Yang
    Departments of Pathology and Cell Biology, University of South Florida College of Medicine, 12901 Bruce B Downs Blvd, Tampa, Florida 33612 4799, USA
    Nat Cell Biol 9:1253-62. 2007
    ....
  65. doi Cell biology: SUMO
    Erik Meulmeester
    Nature 452:709-11. 2008
  66. ncbi Mutual interactions between the SUMO and ubiquitin systems: a plea of no contest
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts, UK
    Trends Cell Biol 15:525-32. 2005
    ..This article gives an overview of target proteins that can serve as substrates for both SUMO and ubiquitin to highlight the diversity of regulatory strategies that result from the crosstalk between the two modification systems...
  67. ncbi Detection of protein SUMOylation in vivo
    Michael H Tatham
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    Nat Protoc 4:1363-71. 2009
    ..In preliminary form, this protocol takes 4-5 d to perform, and it can be further elaborated to provide a multi-angled approach to investigate protein conjugation by SUMO...
  68. ncbi SUMO--nonclassical ubiquitin
    F Melchior
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, Martinsried bei Munchen, D 82152 Germany
    Annu Rev Cell Dev Biol 16:591-626. 2000
    ..While most SUMO targets are still at large, available data provide compelling evidence for a role of SUMO in the regulation of protein-protein interactions and/or subcellular localization...
  69. ncbi SUMO is growing senescent
    Oliver Bischof
    Unité d Organisation Nucléaire et Oncogenèse INSERM U579, Institut Pasteur, Paris, France
    Cell Cycle 6:677-81. 2007
    ..Here, we discuss some of the implications for this novel connection and future directions to study in more detail the importance of the SUMO pathway in senescence and tumorigenesis...
  70. pmc SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis
    Xiang Dong Zhang
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell 29:729-41. 2008
    ..Our findings indicate that SUMOylation is a key regulator of the mammalian cell cycle, with SUMO-1 and SUMO-2/3 modification of different proteins regulating distinct processes...
  71. pmc Parallel SUMOylation-dependent pathways mediate gene- and signal-specific transrepression by LXRs and PPARgamma
    Serena Ghisletti
    Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Mol Cell 25:57-70. 2007
    ..These studies define parallel but functionally distinct pathways that are utilized by PPARgamma and LXRs to differentially regulate complex programs of gene expression that control immunity and homeostasis...
  72. pmc Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance
    Blerta Xhemalce
    Unite de la Dynamique du Génome, Institut Pasteur, Paris Cedex, France
    EMBO J 23:3844-53. 2004
    ..Altogether, these results strongly suggest that Pli1p, and by extension sumoylation, is involved in mechanisms that regulate recombination in particular heterochromatic repeated sequences...
  73. ncbi A functional variant of SUMO4, a new I kappa B alpha modifier, is associated with type 1 diabetes
    Dehuang Guo
    Center for Biotechnology and Genomic Medicine, Medical College of Georgia, 1120 15th Street, CA4098, Augusta, Georgia 30912, USA
    Nat Genet 36:837-41. 2004
    ..5 times greater NF kappa B transcriptional activity and approximately 2 times greater expression of IL12B, an NF kappa B-dependent gene. These findings suggest a new pathway that may be implicated in the pathogenesis of T1D...
  74. ncbi Beginning at the end with SUMO
    Michael J Matunis
    Nat Struct Mol Biol 12:565-6. 2005
  75. ncbi Control of MEF2 transcriptional activity by coordinated phosphorylation and sumoylation
    Serge Gregoire
    Molecular Oncology Group, Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada
    J Biol Chem 281:4423-33. 2006
    ....
  76. ncbi Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3
    Limin Gong
    Department of Cardiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 281:15869-77. 2006
    ..Thus, SENP3 and SENP5 constitute a subfamily of SENPs that regulate the formation of SUMO-2 or SUMO-3 conjugates and, to a less extent, SUMO-1 modification...
  77. ncbi Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast
    Y Takahashi
    Graduate School of Science, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo, 113, Japan
    Biochem Biophys Res Commun 259:582-7. 1999
    ..Around the time of cytokinesis the Cdc3-Smt3 conjugate disappeared. We discuss the biological significance of this Smt3 conjugation to a septin component...
  78. pmc The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer
    E S Johnson
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA
    EMBO J 16:5509-19. 1997
    ..Furthermore, UBA2 and AOS1 are both essential genes, providing additional evidence that they act in a distinct pathway whose role in cell viability is to conjugate Smt3p to other proteins...
  79. pmc Small ubiquitin-related modifier (SUMO) binding determines substrate recognition and paralog-selective SUMO modification
    Jianmei Zhu
    Bloomberg School of Public Health, Department of Biochemistry and Molecular Biology, The Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 283:29405-15. 2008
    ....
  80. ncbi Regulation of p53 family members by the ubiquitin-like SUMO system
    Per Stehmeier
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    DNA Repair (Amst) 8:491-8. 2009
    ..Here we will give a synopsis of the multifaceted effects of SUMO on p53 family members...
  81. ncbi A proteomic strategy for gaining insights into protein sumoylation in yeast
    Carilee Denison
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Proteomics 4:246-54. 2005
    ..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes...
  82. pmc SUSP1 antagonizes formation of highly SUMO2/3-conjugated species
    Debaditya Mukhopadhyay
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 174:939-49. 2006
    ..Together, our findings argue that SUSP1 may play a specialized role in dismantling highly conjugated SUMO2 and -3 species that is critical for PML body maintenance...
  83. ncbi A genome-wide analysis of sumoylation-related biological processes and functions in human nucleus
    Fengfeng Zhou
    National High Performance Computing Center at Hefei, University of Science and Technology of China, Hefei 230027, China
    FEBS Lett 579:3369-75. 2005
    ..g., perception of sound. These potential SUMO substrates in human are of great value for further in vivo or in vitro experimental analysis...
  84. pmc In vivo identification of human small ubiquitin-like modifier polymerization sites by high accuracy mass spectrometry and an in vitro to in vivo strategy
    Ivan Matic
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Mol Cell Proteomics 7:132-44. 2008
    ..The developed methodology is generic and can be adapted for the identification of other sumoylation sites in complex samples...
  85. pmc Association with class IIa histone deacetylases upregulates the sumoylation of MEF2 transcription factors
    Serge Gregoire
    Molecular Oncology Group, Department of Medicine, McGill University Health Centre, 687 Pine Ave West, Montreal, Quebec H3A 1A1, Canada
    Mol Cell Biol 25:2273-87. 2005
    ..These results thus identify sumoylation as a novel regulatory mechanism for MEF2 and suggest that this modification interplays with phosphorylation to promote intramolecular signaling for coordinated regulation in vivo...
  86. pmc Sumo-1 function is dispensable in normal mouse development
    Fu Ping Zhang
    University of Helsinki, Institute of Biomedicine Physiology, Biomedicum Helsinki, P O Box 63 Haartmaninkatu 8, FI 00014 Helsinki, Finland
    Mol Cell Biol 28:5381-90. 2008
    ..Collectively, our results support the notion that most, if not all, SUMO-1 functions are compensated for in vivo by SUMO-2 and SUMO-3...
  87. pmc Extracellular signal-regulated kinase mitogen-activated protein kinase signaling initiates a dynamic interplay between sumoylation and ubiquitination to regulate the activity of the transcriptional activator PEA3
    Baoqiang Guo
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Mol Cell Biol 29:3204-18. 2009
    ....
  88. ncbi SUMO and transcriptional regulation
    David W H Girdwood
    Centre for Biomolecular Sciences, School of Biology, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    Semin Cell Dev Biol 15:201-10. 2004
    ..In most cases, however, SUMO modification of transcription factors leads to repression and various models to explain this, ranging from retention in nuclear bodies to recruitment of histone deacetylases are discussed...
  89. pmc Distinct in vivo dynamics of vertebrate SUMO paralogues
    Ferhan Ayaydin
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
    Mol Biol Cell 15:5208-18. 2004
    ....
  90. pmc Down-regulation of c-Fos/c-Jun AP-1 dimer activity by sumoylation
    Guillaume Bossis
    Institute of Molecular Genetics of Montpellier, UMR5535 IFR122, CNRS 1919, France
    Mol Cell Biol 25:6964-79. 2005
    ..This supports the idea that this modification does not constitute a final inactivation step that necessarily precedes protein degradation...
  91. ncbi Global map of SUMO function revealed by protein-protein interaction and genetic networks
    Taras Makhnevych
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S3E1, Canada
    Mol Cell 33:124-35. 2009
    ....
  92. ncbi Drosophila Ulp1, a nuclear pore-associated SUMO protease, prevents accumulation of cytoplasmic SUMO conjugates
    Matthew Smith
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 90095 1569, USA
    J Biol Chem 279:43805-14. 2004
    ..We hypothesize that, as a component of the nuclear pore complex, Ulp1 may prevent proteins from leaving the nucleus with SUMO still attached...
  93. ncbi A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex
    David Reverter
    Structural Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Structure 12:1519-31. 2004
    ....
  94. ncbi SUMO4 M55V variant is associated with diabetic nephropathy in type 2 diabetes
    Hsing Yi Lin
    Kaohsiung Medical University Hospital, 100 Shih Chuan 1st Rd, Kaohsiung 80708, Taiwan
    Diabetes 56:1177-80. 2007
    ..CONCLUSIONS-This study indicates that the SUMO4 gene M55V variant is associated with severity of diabetic nephropathy in patients with type 2 diabetes...
  95. ncbi Regulation and function of SUMO modification
    Roland S Hilgarth
    Department of Molecular and Cellular Biochemistry and Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 279:53899-902. 2004
  96. ncbi Assessing the validity of the association between the SUMO4 M55V variant and risk of type 1 diabetes
    Deborah J Smyth
    Nat Genet 37:110-1; author reply 112-3. 2005
  97. ncbi Characterization of mouse ubiquitin-like SMT3A and SMT3B cDNAs and gene/pseudogenes
    A Chen
    Department of Biological Sciences, National Sun Yat Sen University, Kaohsiung, Taiwan, ROC
    Biochem Mol Biol Int 46:1161-74. 1998
    ..Here we report the characterization of mouse SMT3A and SMT3B cDNAs, gene/pseudogenes, and mRNA expression...
  98. ncbi Tying SUMO modifications to dynamic behaviors of chromosomes during meiotic prophase of Saccharomyces cerevisiae
    Chun Hsu Cheng
    Institute of Biological Chemistry, Academia Sinica, Taipei, 115, Taiwan
    J Biomed Sci 14:481-90. 2007
    ..This review discusses recent studies related to the role of small ubiquitin-like modifier (SUMO) modification in controlling the overall budding yeast chromosome dynamics during meiotic prophase...
  99. ncbi A proteomic study of SUMO-2 target proteins
    Alfred C O Vertegaal
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 279:33791-8. 2004
    ..SART1 and heterogeneous nuclear RNP M were both shown to be genuine SUMO targets, confirming the validity of the approach...
  100. pmc Protein inhibitor of activated STAT Y (PIASy) and a splice variant lacking exon 6 enhance sumoylation but are not essential for embryogenesis and adult life
    Kelly A Wong
    Molecular and Medical Pharmacology, Howard Hughes Medical Institute, University of California, Los Angeles, 90095, USA
    Mol Cell Biol 24:5577-86. 2004
    ..Our study demonstrates that at steady state, PIASy is either dispensable or compensated for by other PIAS family members or by other mechanisms when deleted...
  101. ncbi Signalling pathways and the regulation of SUMO modification
    B Guo
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, U K
    Biochem Soc Trans 35:1414-8. 2007
    ..Further intricacies in signalling pathway interactions are hinted at through the growing number of examples of cross-talk between different post-translational modifications and SUMO modification...

Research Grants35

  1. A New Family of Voltage-gated Potassium Channel Regulaory Subunits
    Steve A N Goldstein; Fiscal Year: 2011
    ..This revised application seeks to address concerns raised in the first review cycle. ..
  2. PATHOBIOCHEMISTRY OF ACUTE PROMYELOCYTIC LEUKEMIA
    Edward Yeh; Fiscal Year: 2004
    ..These studies should provide novel insights into the mechanism and function of sentrinization and increase our understanding of the pathogenesis of acute promyelocytic leukemia. ..
  3. STUDIES OF THE SENTRIN FAMILY OF UBIQUITIN-LIKE PROTEINS
    Edward Yeh; Fiscal Year: 2000
    ..These studies should provide novel insights into the mechanism and function of sentrinization and increase our understanding of the pathogenesis of acute promyelocytic leukemia. ..
  4. AUTOANTIGEN RNA HELICASE II/GU
    Benigno Valdez; Fiscal Year: 2003
    ..This study may also provide an impetus for understanding the pathological roles of RH-II/Gu on the elicitation of autoimmune responses. ..
  5. Cellular Regulation of Papillomavirus E1 Function
    VAN WILSON; Fiscal Year: 2007
    ..abstract_text> ..
  6. Genetic Screening:Oncogene RAS-Based Inhibi*(RMI)
    Xiaodong Cheng; Fiscal Year: 2005
    ....
  7. NMR STUDIES OF PROTEIN INTERACTIONS IN UBIQUITINATION
    Yuan Chen; Fiscal Year: 2002
    ..abstract_text> ..
  8. Modifiers of Polyglutamine Toxicity
    Andrew Lieberman; Fiscal Year: 2006
    ..Such outcomes will have general impact by furthering our understanding of disease pathogenesis and by yielding a mouse model that faithfully recapitulates the human disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  9. SUMO fusion for genome-wide protein analysis
    TAUSEEF BUTT; Fiscal Year: 2006
    ..Development of a system that enhances expression of proteins and offers rapid purification tools at the genomic level will greatly aid post-genomic expression and protein array technologies. ..
  10. SUMO Fusions to Enhance Expression and Secretion of Protiens
    TAUSEEF BUTT; Fiscal Year: 2007
    ..It will also create an inexpensive platform for endotoxin free protein production that will help the biopharmaceutical industry in the post-genomic era. [unreadable] [unreadable]..
  11. Development of a Novel Method to Enhance Therapeutic Protein Production
    TAUSEEF BUTT; Fiscal Year: 2008
    ..We have proposed the novel use of our previously demonstrated fusion proteins. Such a strategy would likely increase the quantity and quality of protein expression in therapeutics development. [unreadable] [unreadable] [unreadable]..
  12. Regulation of nuclear receptor function through SUMO Modification
    JORGE INIGUEZ LLUHI; Fiscal Year: 2008
    ..abstract_text> ..
  13. The Enzymatic Pathway of a Ubiquitin-Like Modification
    Yuan Chen; Fiscal Year: 2010
    ..abstract_text> ..
  14. Structure/Function of Steroid Receptor AF1 Coregulators
    JORGE INIGUEZ LLUHI; Fiscal Year: 2006
    ..We will then identify mammalian counterparts and begin to ascertain their role in AF1 function using some of the assays outlined in Aims 1 and 2. ..
  15. NMR Studies of Ku70 and Ku80 Domains
    Yuan Chen; Fiscal Year: 2005
    ..This study should also improve our understanding of the structural biology of DNA repair in general. ..
  16. Modifiers of Potassium Channel Function and Expression
    GAIL ROBERTSON; Fiscal Year: 2005
    ..abstract_text> ..
  17. Altered Androgen Receptor Function Due to CAG Expansion
    Andrew Lieberman; Fiscal Year: 2003
    ..Such outcomes will have general impact because this new knowledge will suggest novel targets for the treatment of this and related diseases. ..
  18. Secretion of Ubiquitin-fusion Proteins
    TAUSEEF BUTT; Fiscal Year: 2002
    ..The production of a kit incorporating these improvements could lead to a more reliable method of expressing and purifying proteins from all organisms. ..
  19. REGULATION OF SUMO CONJUGATION TO THE YEAST SEPTINS
    Erica Johnson; Fiscal Year: 2005
    ..Consequently, the fundamental understanding of SUMO conjugation provided by these studies should illuminate the role of SUMO not only in yeast septin function but also in inflammation and cancer. ..
  20. PICORNAVIRAL 3A PROTEINS AND HOST PROTEIN SECRETION
    Karla Kirkegaard; Fiscal Year: 2005
    ..It is anticipated that this method will allow the regulation of the antigen presentation to the cellular immune response by picornaviral-based vectors, which could be crucial in vaccine design. ..
  21. Multivalent Antigen Expression and Vaccine for Malaria
    TAUSEEF BUTT; Fiscal Year: 2003
    ..We believe that a highly efficient method of expression of multiple and ubiquitinated antigens will be a novel solution in the development of malaria vaccine. ..
  22. Identification of Novel Cisplatin Resistance Genes
    Ruea Yea Huang; Fiscal Year: 2005
    ..Evaluation of these targets may identify new biomarkers for pre-treatment screening of tumor samples for mutations that could allow for the identification of patients who are unlikely to benefit from cisplatin treatment. ..
  23. High-Throughput Protein NMR Proteomics
    ATHAN SHAKA; Fiscal Year: 2006
    ..Incorporation of the optimized FDM algorithm into the NMRPipe software suite, supported by NIH, will allow its use in many laboratories. ..
  24. b-TrCP E3 Ligase in CLL
    Benjamin Nicholson; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  25. A high throughput screening assay for the identification of SUMOylation inhibitor
    Yuan Chen; Fiscal Year: 2008
    ..PUBLIC HEALTH RELEVANCE: [unreadable] The overall goal of this proposal is to perform high throughput screening assays (HTS) to identify inhibitors for SUMOylation. [unreadable] [unreadable] [unreadable]..
  26. A Fusion System to Prolong Plasma Half-Life of Proteins
    TAUSEEF BUTT; Fiscal Year: 2005
    ..Development of a new and simple technology that prolongs plasma half-life of proteins will be a major advance in biopharmaceuticals. ..
  27. The Cell Biology of Theiler's Virus Persistence in CNS
    Karla Kirkegaard; Fiscal Year: 2010
    ..Together, this model may prove a paradigm for the functional transfer of infectious material from neurons to oligodendrocytes, and from thence to cells of the immune system. ..
  28. A conditional null mutant of the mouse Npc1 gene
    Andrew Lieberman; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  29. MOLECULAR ANALYSIS OF TRANSCRIPTIONAL REPRESSION BY TGIF
    David Wotton; Fiscal Year: 2010
    ..TGIF is amplified in esophageal tumors, which are more resistant to TGF beta mediated growth inhibition, suggesting a role for TGIF in tumorigenesis. ..
  30. Development of a novel catalytic array
    TAUSEEF BUTT; Fiscal Year: 2007
    ..In Phase II, arrays will be built consisting of all known DUBS for ubiquitin and relevant ubiquitin-like proteins. [unreadable] [unreadable] [unreadable]..
  31. POLIOVIRUS 3D--COOPERATIVE RNA BINDING & POLYMERIZATION
    Karla Kirkegaard; Fiscal Year: 2007
    ..Finally, we provide a genetic rationale for choosing oligomeric proteins as antiviral targets. [unreadable] [unreadable]..
  32. BH3-only protein BNIP3 in tumor progression
    GOVINDASWAMY CHINNADURAI; Fiscal Year: 2006
    ..Our results may also provide the experimental support to suggest that BNIP3 may be a new prognostic marker for human cancer. [unreadable] [unreadable]..
  33. Apoptosis Signaling: BH3 to BH123 Proteins
    GOVINDASWAMY CHINNADURAI; Fiscal Year: 2008
    ..The knowledge gained would be valuable in modulating the activity of BAX in cancer and in degenerative diseases. ..
  34. Isopeptide inhibitors for cancer chemotherapy
    Benjamin Nicholson; Fiscal Year: 2008
    ..Compounds that are shown by the assay to inhibit DEN 1 will be evaluated further, and the best of these will be proposed for further development as antitumor agents. [unreadable] [unreadable] [unreadable]..
  35. GATING MECHANISMS OF HERG AND RELATED CHANNELS
    GAIL ROBERTSON; Fiscal Year: 2002
    ..If the aims of this proposal are achieved a more detailed picture of HERG gating will emerge along with a greater understanding of the physiology of HERG-related channels in the heart. ..