peptide termination factors

Summary

Summary: Proteins that are involved in the peptide chain termination reaction (PEPTIDE CHAIN TERMINATION, TRANSLATIONAL) on RIBOSOMES. They include codon-specific class-I release factors, which recognize stop signals (TERMINATOR CODON) in the MESSENGER RNA; and codon-nonspecific class-II release factors.

Top Publications

  1. pmc Structure of the cross-beta spine of amyloid-like fibrils
    Rebecca Nelson
    Howard Hughes Medical Institute, UCLA DOE Institute for Genomics and Proteomics, Box 951570, UCLA, Los Angeles, California 90095 1570, USA
    Nature 435:773-8. 2005
  2. pmc A systematic survey identifies prions and illuminates sequence features of prionogenic proteins
    Simon Alberti
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 137:146-58. 2009
  3. ncbi An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes
    Jörgen Johansson
    Unité des Interactions Bactéries Cellules, Institut Pasteur, 28, rue du Docteur Roux, 75724 Cedex 15, Paris, France
    Cell 110:551-61. 2002
  4. pmc Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay
    Isao Kashima
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Genes Dev 20:355-67. 2006
  5. pmc A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome
    Ricarda Richter
    Mitochondrial Research Group, Institute for Ageing and Health, Medical School, Newcastle University, Newcastle upon Tyne, UK
    EMBO J 29:1116-25. 2010
  6. pmc The role of ABCE1 in eukaryotic posttermination ribosomal recycling
    Andrey V Pisarev
    Department of Cell Biology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
    Mol Cell 37:196-210. 2010
  7. pmc Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes
    Vera P Pisareva
    Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    EMBO J 30:1804-17. 2011
  8. ncbi Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers
    James Shorter
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 304:1793-7. 2004
  9. ncbi Multiple Gln/Asn-rich prion domains confer susceptibility to induction of the yeast [PSI(+)] prion
    L Z Osherovich
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 106:183-94. 2001
  10. ncbi The physical basis of how prion conformations determine strain phenotypes
    Motomasa Tanaka
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco and California Institute for Quantitative Biomedical Research, San Francisco, California 94143, USA
    Nature 442:585-9. 2006

Research Grants

Detail Information

Publications233 found, 100 shown here

  1. pmc Structure of the cross-beta spine of amyloid-like fibrils
    Rebecca Nelson
    Howard Hughes Medical Institute, UCLA DOE Institute for Genomics and Proteomics, Box 951570, UCLA, Los Angeles, California 90095 1570, USA
    Nature 435:773-8. 2005
    ..The structure illuminates the stability of amyloid fibrils, their self-seeding characteristic and their tendency to form polymorphic structures...
  2. pmc A systematic survey identifies prions and illuminates sequence features of prionogenic proteins
    Simon Alberti
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 137:146-58. 2009
    ..The self-perpetuating states of these proteins present a vast source of heritable phenotypic variation that increases the adaptability of yeast populations to diverse environments...
  3. ncbi An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes
    Jörgen Johansson
    Unité des Interactions Bactéries Cellules, Institut Pasteur, 28, rue du Docteur Roux, 75724 Cedex 15, Paris, France
    Cell 110:551-61. 2002
    ..Strikingly, when the DNA corresponding to the UTR was fused to gfp in E. coli, bacteria became fluorescent at 37 degrees C, but not at 30 degrees C. This mechanism of posttranscriptional thermoregulation may have important applications...
  4. pmc Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay
    Isao Kashima
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Genes Dev 20:355-67. 2006
    ..Thus, the SMG-1-mediated phosphorylation of Upf1 occurs on the association of SURF with EJC, which provides the link between the EJC and recognition of PTCs and triggers NMD...
  5. pmc A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome
    Ricarda Richter
    Mitochondrial Research Group, Institute for Ageing and Health, Medical School, Newcastle University, Newcastle upon Tyne, UK
    EMBO J 29:1116-25. 2010
    ..We suggest that ICT1 may be essential for hydrolysis of prematurely terminated peptidyl-tRNA moieties in stalled mitoribosomes...
  6. pmc The role of ABCE1 in eukaryotic posttermination ribosomal recycling
    Andrey V Pisarev
    Department of Cell Biology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
    Mol Cell 37:196-210. 2010
    ..Importantly, ABCE1 dissociates only post-TCs obtained with eRF1/eRF3 (or eRF1 alone), but not post-TCs obtained with puromycin in eRF1's absence...
  7. pmc Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes
    Vera P Pisareva
    Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    EMBO J 30:1804-17. 2011
    ..ABCE1/Pelota/Hbs1 also dissociated vacant 80S ribosomes, which stimulated 48S complex formation, suggesting that Pelota/Hbs1 have an additional role outside of NGD...
  8. ncbi Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers
    James Shorter
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 304:1793-7. 2004
    ..These Hsp104 activities differed in their reaction mechanism and can explain [PSI+] inheritance patterns...
  9. ncbi Multiple Gln/Asn-rich prion domains confer susceptibility to induction of the yeast [PSI(+)] prion
    L Z Osherovich
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 106:183-94. 2001
    ..These studies suggest a molecular basis for the epigenetic control of [PSI(+)] inducibility and may reveal a broader role for this phenomenon in the physiology of protein aggregation...
  10. ncbi The physical basis of how prion conformations determine strain phenotypes
    Motomasa Tanaka
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco and California Institute for Quantitative Biomedical Research, San Francisco, California 94143, USA
    Nature 442:585-9. 2006
    ..The propensity of aggregates to undergo breakage, thereby generating new seeds, probably represents a key determinant of their physiological impact for both infectious (prion) and non-infectious amyloids...
  11. ncbi In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3
    Elena Z Alkalaeva
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Cell 125:1125-36. 2006
    ..Cooperativity between eRF1 and eRF3 required the eRF3 binding C-terminal domain of eRF1...
  12. pmc Insights into translational termination from the structure of RF2 bound to the ribosome
    Albert Weixlbaumer
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Science 322:953-6. 2008
    ..The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release...
  13. pmc Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions
    James Shorter
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Stellar Chance Laboratories, Philadelphia, PA 19104, USA
    EMBO J 27:2712-24. 2008
    ..This activity is reduced when Ssa1, or enhanced when Ssb1, is incorporated into nascent prions. These findings illuminate several facets of the chaperone interplay that underpins [PSI(+)] inheritance...
  14. pmc Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3
    G Zhouravleva
    Departement de Biologie et Genetique du Developpement, CNRS URA 256, Universite de Rennes, France
    EMBO J 14:4065-72. 1995
    ..It is proposed that a quaternary complex composed of eRF1, eRF3, GTP and a stop codon of the mRNA is involved in termination of polypeptide synthesis in ribosomes...
  15. pmc Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways
    Pavel V Ivanov
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    EMBO J 27:736-47. 2008
    ..The EJC, with UPF2 or UPF3b as a cofactor, interferes with physiological termination through UPF1. This model integrates previously competing models of NMD and suggests a mechanistic basis for alternative NMD pathways...
  16. ncbi The Sup35 domains required for maintenance of weak, strong or undifferentiated yeast [PSI+] prions
    Michael E Bradley
    Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
    Mol Microbiol 51:1649-59. 2004
    ..In view of these findings, we discuss a plausible molecular basis for the [PSI+] prion variants as well as the inherent difficulties in defining a 'prion domain'...
  17. pmc The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs
    K Czaplinski
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School UMDNJ, USA
    Genes Dev 12:1665-77. 1998
    ....
  18. pmc Antagonistic interactions between yeast [PSI(+)] and [URE3] prions and curing of [URE3] by Hsp70 protein chaperone Ssa1p but not by Ssa2p
    Christine Schwimmer
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0851, USA
    Mol Cell Biol 22:3590-8. 2002
    ..Our data also suggest that differences in how [PSI(+)] and [URE3] interact with Hsp104 and Hsp70 may contribute to their antagonistic interactions...
  19. pmc Two-step model of stop codon recognition by eukaryotic release factor eRF1
    Polina Kryuchkova
    Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, 119991 Moscow, Russia
    Nucleic Acids Res 41:4573-86. 2013
    ..Based on our experimental data and molecular modelling of the N-domain at the ribosomal A site, we propose a two-step model of stop codon decoding in the eukaryotic ribosome...
  20. pmc Cryo-EM structure of the mammalian eukaryotic release factor eRF1-eRF3-associated termination complex
    Derek Taylor
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 109:18413-8. 2012
    ..Our structure provides mechanistic insight into the coordination between GTP hydrolysis by eRF3 and subsequent peptide release by eRF1...
  21. pmc Identification of eRF1 residues that play critical and complementary roles in stop codon recognition
    Sara E Conard
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    RNA 18:1210-21. 2012
    ..In particular, changes in the YCF motif, rather than the TASNIKS motif, correlated most consistently with variant code stop codon selectivity...
  22. pmc Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay
    Christopher J Shoemaker
    Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 330:369-72. 2010
    ....
  23. ncbi Structural basis for translation termination on the 70S ribosome
    Martin Laurberg
    Department of Molecular, Cell and Developmental Biology and Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Nature 454:852-7. 2008
    ..Unexpectedly, the main-chain amide group of Gln 230 in the universally conserved GGQ motif of the factor is positioned to contribute directly to peptidyl-tRNA hydrolysis...
  24. pmc The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae
    I Stansfield
    Research School of Biosciences, University of Kent, Canterbury, UK
    EMBO J 14:4365-73. 1995
    ....
  25. pmc Suicidal [PSI+] is a lethal yeast prion
    Ryan P McGlinchey
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0830, USA
    Proc Natl Acad Sci U S A 108:5337-41. 2011
    ..Our findings give a more realistic picture of the impact of the prion change than does focus on "mild" prion variants...
  26. pmc Newly identified prions in budding yeast, and their possible functions
    Emily T Crow
    Department of Molecular Pharmacology and Biological Chemistry, The Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
    Semin Cell Dev Biol 22:452-9. 2011
    ..In this review, we summarize the characteristics of each prion element, and discuss their potential functional roles in yeast biology...
  27. ncbi Specificity of prion assembly in vivo. [PSI+] and [PIN+] form separate structures in yeast
    Sviatoslav Bagriantsev
    Laboratory for Molecular Biology, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 279:51042-8. 2004
    ..These studies demonstrate the intracellular organization of yeast prions and provide insight into the principles of in vivo amyloid assembly...
  28. pmc Contribution of three bile-associated loci, bsh, pva, and btlB, to gastrointestinal persistence and bile tolerance of Listeria monocytogenes
    Maire Begley
    Department of Microbiology, University College Cork, College Road, Cork, Ireland
    Infect Immun 73:894-904. 2005
    ..Finally, animal (murine) studies revealed an important role for both bsh and btlB in the intestinal persistence of L. monocytogenes...
  29. ncbi The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein
    S Hoshino
    Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113 0033, Japan
    J Biol Chem 274:16677-80. 1999
    ..Thus, the present study is the first report showing that GSPT/eRF3 carries the translation termination signal to 3'-poly(A) tail ubiquitously present in eukaryotic mRNAs...
  30. pmc Dependence and independence of [PSI(+)] and [PIN(+)]: a two-prion system in yeast?
    I L Derkatch
    Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
    EMBO J 19:1942-52. 2000
    ..Models for [PIN(+)] action, which explain these findings, are discussed...
  31. ncbi Transcriptome analysis of Listeria monocytogenes identifies three groups of genes differently regulated by PrfA
    Eliane Milohanic
    Unité des Interactions Bactéries Cellules and Laboratoire de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 47:1613-25. 2003
    ..This study reveals that PrfA can act as an activator or a repressor and suggests that PrfA may directly or indirectly activate different sets of genes in association with different sigma factors...
  32. ncbi The essential role of the invariant GGQ motif in the function and stability in vivo of bacterial release factors RF1 and RF2
    Liliana Mora
    UPR9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, Paris 75005, France
    Mol Microbiol 47:267-75. 2003
    ....
  33. ncbi A yeast-based assay to isolate drugs active against mammalian prions
    Stephane Bach
    CNRS, Station Biologique, UPS2682, Place G Teissier, 29680 Roscoff, Bretagne, France
    Methods 39:72-7. 2006
    ..These results strongly argue in favor of common prion controlling mechanisms conserved in eukaryotes, thus validating our yeast-based assay and also the use of budding yeast to identify antiprion compounds and to study the prion world...
  34. pmc Opposing effects of glutamine and asparagine govern prion formation by intrinsically disordered proteins
    Randal Halfmann
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Mol Cell 43:72-84. 2011
    ..Molecular simulations focusing on intrinsic folding differences between Qs and Ns suggest that their different behaviors are due to the enhanced turn-forming propensity of Ns over Qs...
  35. pmc Structural aspects of translation termination on the ribosome
    Andrei A Korostelev
    RNA Therapeutics Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 17:1409-21. 2011
    ..In this review, the structural aspects of these mechanisms are discussed...
  36. pmc How the bacterial pathogen Listeria monocytogenes mediates the switch from environmental Dr. Jekyll to pathogenic Mr. Hyde
    Michael J Gray
    Department of Food Science, Cornell University, Ithaca, NY 14853, USA
    Infect Immun 74:2505-12. 2006
  37. pmc Analysis of the generation and segregation of propagons: entities that propagate the [PSI+] prion in yeast
    Brian Cox
    Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    Genetics 165:23-33. 2003
    ..The implications of our findings with respect to yeast prion propagation mechanisms are discussed...
  38. pmc Primary sequence independence for prion formation
    Eric D Ross
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:12825-30. 2005
    ..These results suggest that [PSI+] formation is driven primarily by the amino acid composition of the Sup35p prion domain, and that the Sup35p oligopeptide repeats are not required for prion maintenance...
  39. ncbi A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor
    L Frolova
    Department of Molecular Biology, University of Aarhus, Denmark
    Nature 372:701-3. 1994
    ..The amino-acid sequence of the eRF1 family is highly conserved. We conclude that the eRF1 proteins are directly implicated in the termination of translation in eukaryotes...
  40. pmc Mutations in C12orf65 in patients with encephalomyopathy and a mitochondrial translation defect
    Hana Antonicka
    Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal H3A 2B4, Quebec, Canada
    Am J Hum Genet 87:115-22. 2010
    ..We suggest that it might play a role in recycling abortive peptidyl-tRNA species, released from the ribosome during the elongation phase of translation...
  41. ncbi Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe
    Chunguang Kong
    Laboratory of Macromolecular Structure, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Japan
    Mol Cell 14:233-45. 2004
    ..The N-terminal extension, rich in acidic amino acids, blocks the proposed eRF1 binding site, potentially regulating eRF1 binding to eRF3 in a competitive manner...
  42. pmc Compositional determinants of prion formation in yeast
    James A Toombs
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    Mol Cell Biol 30:319-32. 2010
    ..Additionally, our results explain why traditional amyloid prediction algorithms fail to accurately predict amyloid formation by the glutamine/asparagine-rich yeast prion domains...
  43. pmc Crystal structure of a translation termination complex formed with release factor RF2
    Andrei Korostelev
    Center for Molecular Biology of RNA and Departments of Molecular, Cell and Developmental Biology and Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Proc Natl Acad Sci U S A 105:19684-9. 2008
    ..We show that when the H-bonding capability of the main-chain N-H of the conserved glutamine is eliminated by substitution with proline, peptidyl-tRNA esterase activity is abolished, consistent with its proposed role in catalysis...
  44. pmc Recognition of the amber UAG stop codon by release factor RF1
    Andrei Korostelev
    Department of Molecular, Cell and Developmental Biology, Center for Molecular Biology of RNA, UCSC, Santa Cruz, CA, USA
    EMBO J 29:2577-85. 2010
    ..These findings are consistent with our proposal that structural rearrangements of RF1 and RF2 are critical to accurate translation termination...
  45. pmc Sequestration of essential proteins causes prion associated toxicity in yeast
    Namitha Vishveshwara
    Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois at Chicago, Chicago, IL 60607, USA
    Mol Microbiol 73:1101-14. 2009
    ..This suggests [PSI(+)] toxicity caused by excess Sup35p verses Sup35NMp is, respectively, through sequestration/inactivation of Sup45p verses Sup35p...
  46. pmc Prion induction involves an ancient system for the sequestration of aggregated proteins and heritable changes in prion fragmentation
    Jens Tyedmers
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 107:8633-8. 2010
    ..Thus, formation of the genetically transmissible prion state is a two-step process that involves an ancient system for the asymmetric inheritance of damaged proteins and heritable changes in the extent of prion fragmentation...
  47. pmc Specificity of the J-protein Sis1 in the propagation of 3 yeast prions
    Takashi Higurashi
    Department of Biochemistry, University of Wisconsin, 445 Biochemistry Addition, 433 Babcock Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:16596-601. 2008
    ....
  48. pmc Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast
    Gloria H Merritt
    Kent Fungal Group and Protein Science Group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    Nucleic Acids Res 38:5479-92. 2010
    ..We reassess current models of stop-codon recognition by eRF1 in the light of these new data...
  49. pmc Hsp104-dependent remodeling of prion complexes mediates protein-only inheritance
    Prasanna Satpute-Krishnan
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, United States of America
    PLoS Biol 5:e24. 2007
    ..Our observations resolve several seemingly conflicting reports on the mechanism of Hsp104 action and point to a single Hsp104-dependent event in prion propagation...
  50. pmc Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition
    Sviatoslav N Bagriantsev
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    Mol Biol Cell 19:2433-43. 2008
    ..Hsp104, Sis1, and Sse1 interact preferentially with the prion versus nonprion form of Sup35, whereas Sla2 and Ssb1/2 interact with both forms of Sup35 with similar efficiency...
  51. pmc Distinct eRF3 requirements suggest alternate eRF1 conformations mediate peptide release during eukaryotic translation termination
    Hua Fan-Minogue
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Cell 30:599-609. 2008
    ..These results suggest that the TASNIKS motif and eRF3 function together to trigger eRF1 conformational changes that couple stop codon recognition and peptide release during eukaryotic translation termination...
  52. ncbi Hungry codons promote frameshifting in human mitochondrial ribosomes
    Richard Temperley
    The Mitochondrial Research Group, Institute for Ageing and Health, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Science 327:301. 2010
    ..By using a sequence-specific endoribonuclease, we show that the rare arginine codons, presumably in association with other cis elements, promote frameshifting in human mitoribosomes...
  53. pmc Destabilizing interactions among [PSI(+)] and [PIN(+)] yeast prion variants
    Michael E Bradley
    Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois, Chicago, Illinois 60607, USA
    Genetics 165:1675-85. 2003
    ..These findings may have implications for understanding interactions among other amyloid-forming proteins, including those associated with certain human diseases...
  54. ncbi [PSI+]: an epigenetic modulator of translation termination efficiency
    T R Serio
    University of Chicago, Department of Molecular Genetics and Cell Biology, Illinois 60637, USA
    Annu Rev Cell Dev Biol 15:661-703. 1999
    ..Here we review the series of experiments supporting the yeast prion hypothesis and provide another look at the 30 years of work preceding this theory in light of our current state of knowledge...
  55. pmc Structural insights into eRF3 and stop codon recognition by eRF1
    Zhihong Cheng
    Cancer and Developmental Cell Biology Division, Institute of Molecular and Cell Biology, Agency for Science, Technology, and Research A STAR, Singapore, Singapore
    Genes Dev 23:1106-18. 2009
    ..An ATP molecule used as a crystallization additive was bound in eRF1's putative decoding area. Mutational analysis of the ATP-binding site shed light on the mechanism of stop codon recognition by eRF1...
  56. pmc A physiological connection between tmRNA and peptidyl-tRNA hydrolase functions in Escherichia coli
    Nongmaithem Sadananda Singh
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India
    Nucleic Acids Res 32:6028-37. 2004
    ..We discuss the physiological relevance of these observations to highlight a major role of tmRNA in decreasing cellular peptidyl-tRNA load...
  57. pmc Highly conserved NIKS tetrapeptide is functionally essential in eukaryotic translation termination factor eRF1
    Ludmila Frolova
    Engelhardt Institute of Molecular Biology, Moscow, Russia
    RNA 8:129-36. 2002
    ..Reduction in ribosome binding revealed for Ile62, Ser64, Arg65, and Arg68 mutants argues in favor of the essential role played by the right part of the NIKS loop in interaction with the ribosome, most probably with ribosomal RNA...
  58. ncbi Kinetic analysis of interaction of eukaryotic release factor 3 with guanine nucleotides
    Vera P Pisareva
    Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
    J Biol Chem 281:40224-35. 2006
    ..Guanine nucleotide binding and exchange on eRF3, which therefore depends on stimulation by eRF1, is entirely different from that on prokaryotic RF3 and unusual among GTPases...
  59. ncbi Epigenetic regulation of translation reveals hidden genetic variation to produce complex traits
    Heather L True
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nature 431:184-7. 2004
    ....
  60. pmc The invariant uridine of stop codons contacts the conserved NIKSR loop of human eRF1 in the ribosome
    Laurent Chavatte
    Institut Jacques Monod, UMR 7592 CNRS Universités Paris 7 Paris 6, 2 place Jussieu, F 75251 Paris Cedex 05, France
    EMBO J 21:5302-11. 2002
    ..Thus, the N and M domains mimic the tRNA anticodon and acceptor arms, respectively...
  61. pmc Crystal structure of the 70S ribosome bound with the Q253P mutant form of release factor RF2
    Natalia Santos
    Center for Molecular Biology of RNA and Department of Molecular, Cell and Developmental Biology, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Structure 21:1258-63. 2013
    ..This rules out proline-induced misfolding and further supports the proposal that catalytic activity requires interaction of the Gln-253 backbone amide with the 3' end of peptidyl-tRNA. ..
  62. ncbi Principles of stop-codon reading on the ribosome
    Johan Sund
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    Nature 465:947-50. 2010
    ..The simulations clearly illustrate the versatility of codon reading by protein, which goes far beyond tRNA mimicry...
  63. pmc Adenine and guanine recognition of stop codon is mediated by different N domain conformations of translation termination factor eRF1
    Konstantin N Bulygin
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia
    Nucleic Acids Res 39:7134-46. 2011
    ..These conformations vary by positioning of stop signal purines toward the universally conserved dipeptide 31-GT-32, which neighbors guanines but is oriented more distantly from adenines...
  64. pmc Codon reassignment in the Escherichia coli genetic code
    Takahito Mukai
    RIKEN Systems and Structural Biology Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Nucleic Acids Res 38:8188-95. 2010
    ..Thus, UAG was assigned unambiguously to a natural or non-natural amino acid, according to the specificity of the UAG-decoding tRNA. The result reveals the unexpected flexibility of the genetic code...
  65. pmc Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases
    Yuji Funakoshi
    Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467 8603, Japan
    Genes Dev 21:3135-48. 2007
    ..Consequently, PABPC1 binding leads to the activation of Pan2-Pan3 and Caf1-Ccr4. From these results, we suggest a mechanism of mRNA deadenylation by Pan2-Pan3 and Caf1-Ccr4 in cooperation with eRF3 and PABPC1...
  66. ncbi Evolution of eukaryotic translation elongation and termination factors: variations of evolutionary rate and genetic code deviations
    David Moreira
    Phylogénie, Bioinformatique et Génome, UMR 7622 CNRS, Paris, France
    Mol Biol Evol 19:189-200. 2002
    ....
  67. ncbi Molecular recognition and catalysis in translation termination complexes
    Bruno P Klaholz
    IGBMC Institute of Genetics and of Molecular and Cellular Biology, Department of Structural Biology and Genomics, Illkirch, France
    Trends Biochem Sci 36:282-92. 2011
    ..Moreover, ongoing research also promises to reveal the structure-function relations of class-II RFs...
  68. pmc Bioinformatic, structural, and functional analyses support release factor-like MTRF1 as a protein able to decode nonstandard stop codons beginning with adenine in vertebrate mitochondria
    David J Young
    Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand
    RNA 16:1146-55. 2010
    ..These observations imply that MTRF1 has the characteristics to recognize A as the first base of a stop codon as would be required to decode the nonstandard codons AGA and AGG...
  69. pmc A size threshold limits prion transmission and establishes phenotypic diversity
    Aaron Derdowski
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 185 Meeting Street, Post Office Box G L2, Providence, RI 02912, USA
    Science 330:680-3. 2010
    ..Thus, prion conformations may specify phenotypes as population averages in a dynamic system...
  70. pmc Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex
    Kan Kobayashi
    Division of Structure Biology, Department of Basic Medical Sciences, The Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 107:17575-9. 2010
    ..Our findings suggest that the molecular mimicry of tRNA in the distorted "A/T state" conformation by Pelota enables the complex to efficiently detect and enter the empty A site of the stalled ribosome...
  71. ncbi Evolution of budding yeast prion-determinant sequences across diverse fungi
    Luke B Harrison
    Department of Biology, McGill University, Stewart Biology Building, 1205 Docteur Penfield Ave, Montreal, QC, Canada H3A 1B1
    J Mol Biol 368:273-82. 2007
    ..Our findings on yeast prion evolution provide further support for the functional significance of these molecules...
  72. pmc Effects of Q/N-rich, polyQ, and non-polyQ amyloids on the de novo formation of the [PSI+] prion in yeast and aggregation of Sup35 in vitro
    Irina L Derkatch
    Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 101:12934-9. 2004
    ..Our data, especially the in vitro reproduction of the highly specific heterologous seeding effect, provide strong support for the hypothesis of cross-seeding in the spontaneous initiation of prion states...
  73. pmc The yeast Sup35NM domain propagates as a prion in mammalian cells
    Carmen Krammer
    Institute of Virology, Technische Universitat Munchen, Trogerstrasse 30, 81675 Munich, Germany
    Proc Natl Acad Sci U S A 106:462-7. 2009
    ..The fact that the yeast Sup35NM domain can propagate as a prion in neuroblastoma cells strongly argues that cellular mechanisms support prion-like inheritance in the mammalian cytosol...
  74. ncbi Prion domains: sequences, structures and interactions
    Eric D Ross
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0830, USA
    Nat Cell Biol 7:1039-44. 2005
    ..Ure2p and Sup35p, two yeast prion proteins, can still form prions when the prion domains are shuffled, indicating a parallel in-register beta-sheet structure...
  75. ncbi Peptidyl-tRNA regulates the GTPase activity of translation factors
    Andrey V Zavialov
    Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Cell 114:113-22. 2003
    ..We also propose a model for translocation of tRNAs in two separate steps, which clarifies the roles of EF-G.GTP and GTP hydrolysis in this process...
  76. ncbi Yeast [PSI+] "prions" that are crosstransmissible and susceptible beyond a species barrier through a quasi-prion state
    T Nakayashiki
    Department of Basic Medical Sciences and, Institute of Medical Science, University of Tokyo, 108 8639, Tokyo, Japan
    Mol Cell 7:1121-30. 2001
    ..cerevisiae but in K. lactis. These two Sup35 proteins contain unique multiple imperfect oligopeptide repeats responsible for crosstransmission and high spontaneous propagation of novel [PSI(+)] elements...
  77. pmc Saccharomyces cerevisiae Hsp70 mutations affect [PSI+] prion propagation and cell growth differently and implicate Hsp40 and tetratricopeptide repeat cochaperones in impairment of [PSI+]
    Gary W Jones
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0851, USA
    Genetics 163:495-506. 2003
    ..Other suppressing mutations are in residues important for interactions with Hsp40 or TPR-containing cochaperones, suggesting that such interactions are necessary for the impairment of [PSI(+)] propagation caused by mutant Ssa1p...
  78. pmc Appearance and propagation of polyglutamine-based amyloids in yeast: tyrosine residues enable polymer fragmentation
    Ilya M Alexandrov
    Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street, 121552 Moscow, Russia
    J Biol Chem 283:15185-92. 2008
    ..The presence of tyrosines within polyglutamine stretches dramatically enhanced polymer fragmentation and allowed polymer propagation in the absence of Rnq1 and, in some cases, of Hsp104...
  79. ncbi A tripeptide 'anticodon' deciphers stop codons in messenger RNA
    K Ito
    Department of Tumor Biology, Institute of Medical Science, University of Tokyo, Japan
    Nature 403:680-4. 2000
    ..These findings show that the discriminator tripeptide of bacterial release factors is functionally equivalent to that of the anticodon of transfer RNA, irrespective of the difference between protein and RNA...
  80. pmc Glycerol metabolism and PrfA activity in Listeria monocytogenes
    Biju Joseph
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Gebäude E1, 97080 Wurzburg, Germany
    J Bacteriol 190:5412-30. 2008
    ..These and other data suggest that the phosphorylation state of PTS permeases correlates with PrfA activity...
  81. pmc Genetic and epigenetic control of the efficiency and fidelity of cross-species prion transmission
    Buxin Chen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332 0230, USA
    Mol Microbiol 76:1483-99. 2010
    ..Individual amino acid substitutions within short amyloidogenic stretches drastically alter patterns of cross-species prion conversion, implicating these stretches as major determinants of species specificity...
  82. pmc Different modes of stop codon restriction by the Stylonychia and Paramecium eRF1 translation termination factors
    Sergey Lekomtsev
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia
    Proc Natl Acad Sci U S A 104:10824-9. 2007
    ....
  83. ncbi A trans-acting riboswitch controls expression of the virulence regulator PrfA in Listeria monocytogenes
    Edmund Loh
    Department of Molecular Biology, Umea University, 90187 Umea, Sweden
    Cell 139:770-9. 2009
    ..Together, our results uncover an unexpected role for riboswitches and a distinct class of regulatory noncoding RNAs in bacteria...
  84. ncbi Survey on the PABC recognition motif PAM2
    Mario Albrecht
    Max Planck Institute for Informatics, Stuhlsatzenhausweg 85, Saarbrucken 66123, Germany
    Biochem Biophys Res Commun 316:129-38. 2004
    ..It is also striking that the PAM2 motif appears to occur solely outside globular protein domains...
  85. pmc Invariant amino acids essential for decoding function of polypeptide release factor eRF1
    Petr Kolosov
    Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, 119991 Moscow, Russia
    Nucleic Acids Res 33:6418-25. 2005
    ..The data point to a pivotal role played by the YxCxxxF motif (positions 125-131) in purine discrimination of the stop codons. We speculate that eRF1 decoding site is formed by a 3D network of amino acids side chains...
  86. ncbi Single mother-daughter pair analysis to clarify the diffusion properties of yeast prion Sup35 in guanidine-HCl-treated [PSI] cells
    Shigeko Kawai-Noma
    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277 8562, Japan
    Genes Cells 14:1045-54. 2009
    ..The noninvasive methods described here can be applied to other protein-based transmissible systems inside living cells...
  87. ncbi RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer
    M R Culbertson
    Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
    Trends Genet 15:74-80. 1999
    ..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
  88. pmc The iron-sulphur protein RNase L inhibitor functions in translation termination
    Sohail Khoshnevis
    Abteilung für Molekulare Strukturbiologie, Institut fur Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg August Universitat Gottingen, Gottingen, Germany
    EMBO Rep 11:214-9. 2010
    ....
  89. ncbi Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon
    Sabine Petry
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 123:1255-66. 2005
    ..Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway...
  90. pmc Analyzing the birth and propagation of two distinct prions, [PSI+] and [Het-s](y), in yeast
    Vidhu Mathur
    Department of Biological Sciences, University of Illinois, Chicago, IL 60607, USA
    Mol Biol Cell 21:1449-61. 2010
    ..Both [PSI(+)] and [Het-s](y) first appear in daughters as numerous tiny dot-like aggregates, and both require the endocytic protein, Sla2, for ring formation, but not propagation...
  91. pmc Release factor 2 frameshifting sites in different bacteria
    Pavel V Baranov
    Department of Human Genetics, University of Utah, 15N 2030E Room 7410, Salt Lake City, UT 84112 5330, USA
    EMBO Rep 3:373-7. 2002
    ..Internal translation initiation occurs at the shift site, but any functional role is obscure...
  92. pmc Three distinct peptides from the N domain of translation termination factor eRF1 surround stop codon in the ribosome
    Konstantin N Bulygin
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
    RNA 16:1902-14. 2010
    ..Thus, in the A-site-bound state, the eRF1 conformation significantly differs from those in crystals and solution. The model suggested for eRF1 conformation in the ribosomal A site and cross-linking data are compatible...
  93. ncbi Mouse GSPT2, but not GSPT1, can substitute for yeast eRF3 in vivo
    Catherine Le Goff
    Universite de Rennes 1, CNRS UMR 6061, IFR 97, 2 av Pr Léon Bernard 35043 Rennes Cedex, France
    Genes Cells 7:1043-57. 2002
    ..The termination of protein synthesis in eukaryotes involves at least two polypeptide release factors (eRFs), eRF1 and eRF3. In mammals two genes encoding eRF3 structural homologues were identified and named GSPT1 and GSPT2...
  94. pmc Conversion of omnipotent translation termination factor eRF1 into ciliate-like UGA-only unipotent eRF1
    Alim Seit-Nebi
    Engelhardt Institute of Molecular Biology, Moscow, Russia
    EMBO Rep 3:881-6. 2002
    ..We assume that stop codon recognition is profoundly different by eukaryotic and prokaryotic class 1 RFs...
  95. pmc Effect of acid adaptation on the fate of Listeria monocytogenes in THP-1 human macrophages activated by gamma interferon
    Maria Pia Conte
    Department of Public Health Sciences, University La Sapienza, 00185 Rome, Italy
    Infect Immun 70:4369-78. 2002
    ..Our data indicate that preexposure to a low pH has a positive impact on subsequent challenge of L. monocytogenes with macrophagic cells...
  96. pmc A single amino acid change of translation termination factor eRF1 switches between bipotent and omnipotent stop-codon specificity
    Boris Eliseev
    Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, Moscow, Russia
    Nucleic Acids Res 39:599-608. 2011
    ..aediculatus eRF1 to omnipotent mode is due to a single point mutation. Furthermore, we examined the influence of eRF3 on the ability of chimeric and mutant eRF1s to induce peptide release in response to different stop codons...
  97. ncbi Solution structure of the catalytic domain of the mitochondrial protein ICT1 that is essential for cell vitality
    Yoshihiro Handa
    Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 1 5 1 Tenjin cho, Kiryu shi, Gunma 376 8515, Japan
    J Mol Biol 404:260-73. 2010
    ..In addition, cytochrome c oxidase activity in ICT1 knockdown cells was decreased by 35% compared to that in control cells. These results indicate that ICT1 function is essential for cell vitality and mitochondrial function...
  98. ncbi A tripeptide discriminator for stop codon recognition
    Yoshikazu Nakamura
    Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, 108 8630, Tokyo, Japan
    FEBS Lett 514:30-3. 2002
    ..Here we review the experimental background and process leading to this discovery, and strengthen functional evidence for the tripeptide determinant for deciphering stop codons in mRNAs in prokaryotes...
  99. pmc Molecular chaperone Hsp104 can promote yeast prion generation
    Dmitry S Kryndushkin
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0830, USA
    Genetics 188:339-48. 2011
    ..The same factor may both enhance de novo prion generation and destabilize existing prion variants, suggesting that prion variants may be selected by changes in the chaperone network...
  100. ncbi Protein synthesis factors (RF1, RF2, RF3, RRF, and tmRNA) and peptidyl-tRNA hydrolase rescue stalled ribosomes at sense codons
    Serafín Vivanco-Domínguez
    Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, P O Box 14 740, Mexico City, 07000, Mexico
    J Mol Biol 417:425-39. 2012
    ..This is followed by the disassembly of the ribosomal complex of tRNA and mRNA by RRF and elongation factor G...
  101. pmc Interaction between yeast Sup45p (eRF1) and Sup35p (eRF3) polypeptide chain release factors: implications for prion-dependent regulation
    S V Paushkin
    Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia
    Mol Cell Biol 17:2798-805. 1997
    ..This phenomenon represents a novel mechanism of regulation of gene expression at the posttranslational level...

Research Grants19

  1. Strutural basis of amyloid fiber pathology
    Ruben Diaz Avalos; Fiscal Year: 2003
    ..abstract_text> ..
  2. Preventing Human Prion Diseases - Inactivation of Prions
    Stanley B Prusiner; Fiscal Year: 2010
    ..Effective protocols for inactivating prions will protect the general public as well as laboratory scientists who are investigating prions. Rarely does a research study have such immediate and important implications. ..
  3. Surface protein dynamics in live bacterial pathogens
    Julie A Theriot; Fiscal Year: 2010
    ..abstract_text> ..
  4. ACTIN BASED MOTILITY OF A BACTERIAL PATHOGEN
    Julie Theriot; Fiscal Year: 2009
    ..abstract_text> ..
  5. Mechanism of Eukaryotic Translation Termination
    David M Bedwell; Fiscal Year: 2011
    ..The third aim of this proposal will test various aspects of this model so we can better understand the important interplay between translation termination and mRNA stability. ..
  6. Development & Applications of Knowledge-based Potentials
    Yaoqi Zhou; Fiscal Year: 2006
    ..The successful completion of the proposed studies will likely lead to a new generation of algorithms for more accurate structure determination. ..
  7. Structure-based prediction of folding mechanism
    Yaoqi Zhou; Fiscal Year: 2007
    ..The analytic tools and computational methods developed in the proposal have the potential to be widely used by those who are interested in determining the preferred folding pathways. ..
  8. UNIQUE AMINO DOMAINS AND AMP DEAMINASE ISOFORM DIVERSITY
    RICHARD SABINA; Fiscal Year: 2002
    ..These combined efforts should provide critical information that may help explain clinical outcomes associated with a prevalent AMPD1 mutant allele. ..
  9. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    Stephen Goff; Fiscal Year: 2003
    ..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
  10. Genetic Study of the Yeast Prion-Interacting Proteins
    Yury O Chernoff; Fiscal Year: 2010
    ..This research will further establish yeast as a model for development of the strategies counteracting accumulation and propagation of prions and other toxic amyloids. ..
  11. Srv is critical for Streptococcal survival in the host
    SEAN REID; Fiscal Year: 2009
    ..Understanding the mechanism of Srv-dependent gene regulation will provide new insights into the control of GAS disease. ..
  12. Elucidating the Function of Novel Epigenetic Element
    HEATHER TRUE KROB; Fiscal Year: 2005
    ....
  13. The Causes and Consequences of Complementation and Selfishness in Viruses
    Sarah Joseph; Fiscal Year: 2008
    ..As a result, understanding these interactions in bacteriophage may provide insights into the evolution and pathogenesis of viral pathogens of humans. [unreadable] [unreadable] [unreadable]..
  14. Structural studies on ribosomes and antibiotics
    VENKATRAMAN RAMAKRISHNAN; Fiscal Year: 2006
    ..Such knowledge could help improve existing antibiotics and could also lead to the design of new ones. ..
  15. Nucleotide Methylation in Ribosome Maturation
    JASON RIFE; Fiscal Year: 2008
    ..Ultimately, these studies will reveal molecular details of methyltransferase reactions as well as likely provide the first evidence for a universal step in ribosome biogenesis. ..
  16. GENES AND GENETIC INTERACTIONS UNDERLYING PHARMACOLOGICAL VARIATION IN YEAST
    Weixiong Zhang; Fiscal Year: 2010
    ..The proposed research will develop and evaluate a method of identifying multiple genes that contribute to complex traits and a model to predict their dependence on genetic background using gene expression data. ..
  17. Biofilm Formation by Listeria monocytogenes
    KATHERINE LEMON; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  18. Towards a general theory of evolutionary capacitance
    Joanna Masel; Fiscal Year: 2009
    ..Since direct experiments on capacitance cannot be performed in humans, the theoretical and predictive approach described here is particularly important for understanding the scope of capacitance in explaining human variation. ..