ribosomal proteins

Summary

Summary: Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.

Top Publications

  1. ncbi The economics of ribosome biosynthesis in yeast
    J R Warner
    Dept of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, Bronx, NY 10461, USA
    Trends Biochem Sci 24:437-40. 1999
  2. ncbi Assembly of bacterial ribosomes
    Zahra Shajani
    Departments of Molecular Biology and Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Biochem 80:501-26. 2011
  3. ncbi The post-transcriptional steps of eukaryotic ribosome biogenesis
    A K Henras
    Laboratoire de Biologie Moleculaire Eucaryote, CNRS, Universite de Toulouse, UPS, 118 route de Narbonne, 31062 Toulouse Cedex 09, France
    Cell Mol Life Sci 65:2334-59. 2008
  4. ncbi Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1
    Julius Rabl
    Institute of Molecular Biology and Biophysics, ETH Zurich, Schafmattstrasse 20, 8093 Zurich, Switzerland
    Science 331:730-6. 2011
  5. pmc Suprainduction of p53 by disruption of 40S and 60S ribosome biogenesis leads to the activation of a novel G2/M checkpoint
    Stefano Fumagalli
    Division of Hematology and Oncology, Department of Internal Medicine, College of Medicine, Metabolic Diseases Institute, University of Cincinnati, Cincinnati, Ohio 45237, USA
    Genes Dev 26:1028-40. 2012
  6. ncbi What recent ribosome structures have revealed about the mechanism of translation
    T Martin Schmeing
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Nature 461:1234-42. 2009
  7. ncbi Crystal structure of the eukaryotic ribosome
    Adam Ben-Shem
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Science 330:1203-9. 2010
  8. ncbi Toward automatic reconstruction of a highly resolved tree of life
    Francesca D Ciccarelli
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany
    Science 311:1283-7. 2006
  9. ncbi Signaling to p53: ribosomal proteins find their way
    Yanping Zhang
    Department of Radiation Oncology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7512, USA
    Cancer Cell 16:369-77. 2009
  10. ncbi Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6
    Sebastian Klinge
    Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, Switzerland
    Science 334:941-8. 2011

Detail Information

Publications320 found, 100 shown here

  1. ncbi The economics of ribosome biosynthesis in yeast
    J R Warner
    Dept of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, Bronx, NY 10461, USA
    Trends Biochem Sci 24:437-40. 1999
    ..devoted to ribosomal RNA, and 50% of RNA polymerase II transcription and 90% of mRNA splicing are devoted to ribosomal proteins (RPs)...
  2. ncbi Assembly of bacterial ribosomes
    Zahra Shajani
    Departments of Molecular Biology and Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Biochem 80:501-26. 2011
    ..an alternating series of RNA conformational changes and protein-binding events; we explore the effects of ribosomal proteins in driving these events...
  3. ncbi The post-transcriptional steps of eukaryotic ribosome biogenesis
    A K Henras
    Laboratoire de Biologie Moleculaire Eucaryote, CNRS, Universite de Toulouse, UPS, 118 route de Narbonne, 31062 Toulouse Cedex 09, France
    Cell Mol Life Sci 65:2334-59. 2008
    ..It involves the transcription and processing of pre-ribosomal RNAs, their proper folding and assembly with ribosomal proteins and the transport of the resulting pre-ribosomal particles to the cytoplasm where final maturation events ..
  4. ncbi Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1
    Julius Rabl
    Institute of Molecular Biology and Biophysics, ETH Zurich, Schafmattstrasse 20, 8093 Zurich, Switzerland
    Science 331:730-6. 2011
    ..9 angstroms. The structure reveals the fold of the entire 18S ribosomal RNA and of all ribosomal proteins of the 40S subunit, and defines the interactions with eIF1...
  5. pmc Suprainduction of p53 by disruption of 40S and 60S ribosome biogenesis leads to the activation of a novel G2/M checkpoint
    Stefano Fumagalli
    Division of Hematology and Oncology, Department of Internal Medicine, College of Medicine, Metabolic Diseases Institute, University of Cincinnati, Cincinnati, Ohio 45237, USA
    Genes Dev 26:1028-40. 2012
    ..Induction of p53 is attributed to the binding and inhibition of human double minute 2 (Hdm2) by a subset of ribosomal proteins (RPs): RPS7, RPL5, RPL11, and RPL23...
  6. ncbi What recent ribosome structures have revealed about the mechanism of translation
    T Martin Schmeing
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Nature 461:1234-42. 2009
    ..In this review, we discuss how the interaction between structural and functional studies over the last decade has led to a deeper understanding of the complex mechanisms underlying translation...
  7. ncbi Crystal structure of the eukaryotic ribosome
    Adam Ben-Shem
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Science 330:1203-9. 2010
    ..We describe the conformational changes in both ribosomal subunits that are involved in ratcheting and their implications in coordination between the two associated subunits and in mRNA and tRNA translocation...
  8. ncbi Toward automatic reconstruction of a highly resolved tree of life
    Francesca D Ciccarelli
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany
    Science 311:1283-7. 2006
    ..For example, we place the phylum Acidobacteria as a sister group of delta-Proteobacteria, support a Gram-positive origin of Bacteria, and suggest a thermophilic last universal common ancestor...
  9. ncbi Signaling to p53: ribosomal proteins find their way
    Yanping Zhang
    Department of Radiation Oncology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7512, USA
    Cancer Cell 16:369-77. 2009
    ..In response to this stress, several ribosomal proteins bind to MDM2 and block MDM2-mediated p53 ubiquitination and degradation, resulting in p53-dependent cell ..
  10. ncbi Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6
    Sebastian Klinge
    Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, Switzerland
    Science 334:941-8. 2011
    ..Furthermore, it elucidates the molecular basis of the interaction with eIF6 and provides a structural framework for further studies of ribosome-associated diseases and the role of the 60S subunit in the initiation of protein synthesis...
  11. pmc A cluster of ribosome synthesis factors regulate pre-rRNA folding and 5.8S rRNA maturation by the Rat1 exonuclease
    Sander Granneman
    Wellcome Trust Centre for Cell Biology and Centre for Systems Biology at Edinburgh, University of Edinburgh, Edinburgh, Scotland
    EMBO J 30:4006-19. 2011
    ..We predict that Nop4 and the A(3) cluster establish long-range interactions between the 5.8S and 25S rRNAs, which are subsequently maintained by ribosomal protein binding...
  12. ncbi Ribosome-mediated specificity in Hox mRNA translation and vertebrate tissue patterning
    Nadya Kondrashov
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, 94158, USA
    Cell 145:383-97. 2011
    ..Collectively, these findings suggest that RP activity may be highly regulated to impart a new layer of specificity in the control of gene expression and mammalian development...
  13. ncbi Regulation of HDM2 activity by the ribosomal protein L11
    Marion A E Lohrum
    Regulation of Cell Growth Laboratory, NCI FRCDC, Frederick, MD 21702, USA
    Cancer Cell 3:577-87. 2003
    ....
  14. pmc Ribosome deficiency protects against ER stress in Saccharomyces cerevisiae
    Kristan K Steffen
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Genetics 191:107-18. 2012
    In Saccharomyces cerevisiae, 59 of the 78 ribosomal proteins are encoded by duplicated genes that, in most cases, encode identical or very similar protein products...
  15. ncbi Crystal structure of the 80S yeast ribosome
    Lasse Jenner
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Curr Opin Struct Biol 22:759-67. 2012
    ..Finally we will discuss the impact of the yeast 80S ribosome crystal structure on perspectives for future investigations...
  16. ncbi Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia
    Hidetsugu Torihara
    Frontier Science Research Centre, University of Miyazaki, Kiyotake, Miyazaki, Japan
    Br J Haematol 152:648-54. 2011
    ..These results suggest that a Tp53-independent but RPS19-dependent pathway could be responsible for defective erythropoiesis in RPS19-deficient zebrafish...
  17. pmc Phylogenomics of prokaryotic ribosomal proteins
    Natalya Yutin
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 7:e36972. 2012
    ..These alignments, available for download, can be used as search profiles to improve genome annotation of r-proteins and for further comparative genomics studies...
  18. pmc Structures of the bacterial ribosome in classical and hybrid states of tRNA binding
    Jack A Dunkle
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Science 332:981-4. 2011
    ..The structures help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and ribosome recycling...
  19. pmc Ribosome assembly factors prevent premature translation initiation by 40S assembly intermediates
    Bethany S Strunk
    Chemical Biology Doctoral Program, University of Michigan, Ann Arbor, MI 48109, USA
    Science 333:1449-53. 2011
    ..These redundant mechanisms probably ensure that pre-40S particles do not enter the translation pathway, which would result in their rapid degradation...
  20. pmc How common are extraribosomal functions of ribosomal proteins?
    Jonathan R Warner
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Cell 34:3-11. 2009
    b>Ribosomal proteins are ubiquitous, abundant, and RNA binding: prime candidates for recruitment to extraribosomal functions. Indeed, they participate in balancing the synthesis of the RNA and protein components of the ribosome itself...
  21. ncbi Ribosomal protein S19 deficiency in zebrafish leads to developmental abnormalities and defective erythropoiesis through activation of p53 protein family
    Nadia Danilova
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, UCLA, USA
    Blood 112:5228-37. 2008
    Mutations in several ribosomal proteins (RPs) lead to Diamond-Blackfan anemia (DBA), a syndrome characterized by defective erythropoiesis, congenital anomalies, and increased frequency of cancer...
  22. ncbi Structure of the 70S ribosome complexed with mRNA and tRNA
    Maria Selmer
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 313:1935-42. 2006
    ..The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation...
  23. ncbi Regulation of the MDM2-P53 pathway and tumor growth by PICT1 via nucleolar RPL11
    Masato Sasaki
    Global Centers of Excellence Program, Akita University Graduate School of Medicine, Akita, Japan
    Nat Med 17:944-51. 2011
    ..When PICT1 is depleted in tumor cells with intact P53 signaling, the cells grow more slowly and accumulate P53. Thus, PICT1 is a potent regulator of the MDM2-P53 pathway and promotes tumor progression by retaining RPL11 in the nucleolus...
  24. ncbi Growth control and ribosome biogenesis
    Harri Lempiäinen
    Department of Molecular Biology and NCCR Program Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4, Switzerland
    Curr Opin Cell Biol 21:855-63. 2009
    ..Transcription of ribosomal RNA is finely tuned to cellular energy status and linked to the production of ribosomal proteins. Several autoregulatory mechanisms controlling various aspects of ribosome biogenesis have been uncovered ..
  25. pmc Haploinsufficiency for ribosomal protein genes causes selective activation of p53 in human erythroid progenitor cells
    Shilpee Dutt
    Division of Hematology, Brigham and Women s Hospital, Boston, MA, USA
    Blood 117:2567-76. 2011
    ..Our findings indicate that the erythroid lineage has a low threshold for the induction of p53, providing a basis for the failure of erythropoiesis in the 5q-syndrome, DBA, and perhaps other bone marrow failure syndromes...
  26. pmc Ribosomal protein L11 negatively regulates oncoprotein MDM2 and mediates a p53-dependent ribosomal-stress checkpoint pathway
    Yanping Zhang
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Cell Biol 23:8902-12. 2003
    ..We suggest that L11 functions as a negative regulator of HDM2 and that there might exist in vivo an L11-HDM2-p53 pathway for monitoring ribosomal integrity...
  27. pmc A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae
    L Ni
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Biol Cell 12:2147-70. 2001
    ..Genome-wide screens of defined collections of mutants hold significant promise for dissecting many biological processes in yeast...
  28. pmc Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
    Yun Wah Lam
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Biol 17:749-60. 2007
    The nucleolus is a subnuclear organelle in which rRNAs are transcribed, processed, and assembled with ribosomal proteins into ribosome subunits...
  29. pmc Integrity of the P-site is probed during maturation of the 60S ribosomal subunit
    Cyril Bussiere
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 197:747-59. 2012
    ..We propose that Efl1 promotes a functional check of the integrity of the 60S subunit before its first round of translation...
  30. ncbi Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin
    Masatoshi Takagi
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell 123:49-63. 2005
    ..These findings demonstrate the importance of increased translation of p53 in DNA-damage responses and suggest critical roles for RPL26 and nucleolin in affecting p53 induction...
  31. pmc The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly
    Jochen Bassler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Mol Cell 38:712-21. 2010
    ....
  32. ncbi Atomic structures of the eukaryotic ribosome
    Sebastian Klinge
    Institute of Molecular Biology and Biophysics, ETH Zurich, 8093 Zurich, Switzerland
    Trends Biochem Sci 37:189-98. 2012
    ..At the same time, it facilitates a direct comparison between prokaryotic and eukaryotic ribosomal features...
  33. pmc Ribosomal protein S7 is both a regulator and a substrate of MDM2
    Yan Zhu
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell 35:316-26. 2009
    ..This indicates that MDM2 ubiquitination of S7 is involved in sustaining the p53 response. Thus, S7 functions as both effector and affector of MDM2 to ensure a proper cellular response to different stress signals...
  34. ncbi PML regulates p53 stability by sequestering Mdm2 to the nucleolus
    Rosa Bernardi
    Cancer Biology and Genetics Program, Department of Pathology and Medicine, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Nat Cell Biol 6:665-72. 2004
    ..These findings demonstrate an unexpected role of PML in the nucleolar network for tumour suppression...
  35. ncbi Inhibition of MDM2-mediated p53 ubiquitination and degradation by ribosomal protein L5
    Mu Shui Dai
    Department of Biochemistry and Molecular Biology, School of Medicine, Oregon Health and Science University, Portland, Oregon 97201, USA
    J Biol Chem 279:44475-82. 2004
    The oncoprotein MDM2 associates with ribosomal proteins L5, L11, and L23. Both L11 and L23 have been shown to activate p53 by inhibiting MDM2-mediated p53 suppression. Here we have shown that L5 also activates p53...
  36. pmc A rooted net of life
    David Williams
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    Biol Direct 6:45. 2011
    ..Given sufficient evidence, edges may then be differentiated as those representing vertical lines of inheritance within lineages and those representing horizontal genetic transfers or endosymbioses between lineages...
  37. pmc Comparative analysis of ribosomal proteins in complete genomes: an example of reductive evolution at the domain scale
    Odile Lecompte
    Laboratoire de Biologie et Genomique Structurales, Institut de Génétique et de Biologie Moléculaire et Cellulaire CNRS, INSERM, ULP, BP163, 67404 Illkirch Cedex, France
    Nucleic Acids Res 30:5382-90. 2002
    ..This first documented case of reductive evolution at the domain scale provides a new framework for discussing the shape of the universal tree of life and the selective forces directing the evolution of prokaryotes...
  38. pmc Powering through ribosome assembly
    Bethany S Strunk
    Chemical Biology Doctoral Program, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    RNA 15:2083-104. 2009
    ..of the biophysical, thermodynamic, and structural basis for the ordered and correct assembly of ribosomal proteins on ribosomal RNA...
  39. pmc Inhibition of HDM2 and activation of p53 by ribosomal protein L23
    Aiwen Jin
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, USA
    Mol Cell Biol 24:7669-80. 2004
    ....
  40. pmc Maturation of eukaryotic ribosomes: acquisition of functionality
    Vikram Govind Panse
    Institute of Biochemistry IBC, ETH Zurich, CH 8093, Zurich, Switzerland
    Trends Biochem Sci 35:260-6. 2010
    ..involves the release of trans-acting shuttling factors, transport factors, incorporation of the remaining ribosomal proteins, and final rRNA processing steps...
  41. pmc Ribosome biogenesis and the translation process in Escherichia coli
    Magdalena Kaczanowska
    Department of Genetics, Microbiology, and Toxicology, Stockholm University, S 10691 Stockholm, Sweden
    Microbiol Mol Biol Rev 71:477-94. 2007
    ....
  42. ncbi Ribosome biogenesis surveillance: probing the ribosomal protein-Mdm2-p53 pathway
    C Deisenroth
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Oncogene 29:4253-60. 2010
    ..is a complex process comprising transcription, modification, and processing of ribosomal RNA, production of ribosomal proteins (RPs) and auxiliary factors, and coordinated assembly of ribonucleoprotein particles to produce mature ..
  43. pmc Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition
    Mu Shui Dai
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201, USA
    Mol Cell Biol 24:7654-68. 2004
    ..These results reveal that L23 is another regulator of the p53-MDM2 feedback regulation...
  44. ncbi Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation
    Jingyi Fei
    Department of Chemistry, Columbia University, New York, NY 10027, USA
    Mol Cell 30:348-59. 2008
    ..Real-time smFRET experiments reveal that the L1 stalk-tRNA interaction persists throughout the translocation reaction, suggesting that the L1 stalk acts to direct tRNA movements during translocation...
  45. pmc Ribosomal protein S14 unties the MDM2-p53 loop upon ribosomal stress
    X Zhou
    Department of Biochemistry and Molecular Biology and Cancer Center, Indiana University School of Medicine, Indianapolis, IN, USA
    Oncogene 32:388-96. 2013
    ..Several ribosomal proteins, such as RPL11, RPL5, RPL23, RPL26 or RPS7, have been shown to have a role in regulation of this feedback ..
  46. pmc Mutual protection of ribosomal proteins L5 and L11 from degradation is essential for p53 activation upon ribosomal biogenesis stress
    Sladana Bursac
    Department of Molecular Medicine and Biotechnology, School of Medicine, University of Rijeka, 51000 Rijeka, Croatia
    Proc Natl Acad Sci U S A 109:20467-72. 2012
    Impairment of ribosomal biogenesis can activate the p53 protein independently of DNA damage. The ability of ribosomal proteins L5, L11, L23, L26, or S7 to bind Mdm2 and inhibit its ubiquitin ligase activity has been suggested as a ..
  47. pmc Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae
    Jason Talkish
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Nucleic Acids Res 40:8646-61. 2012
    ..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes...
  48. pmc Ribosomal proteins L7 and L8 function in concert with six A₃ assembly factors to propagate assembly of domains I and II of 25S rRNA in yeast 60S ribosomal subunits
    Jelena Jakovljevic
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    RNA 18:1805-22. 2012
    ..process that involves alternating steps of folding and processing of pre-rRNAs in concert with assembly of ribosomal proteins. Recently, there has been increased interest in the roles of ribosomal proteins in eukaryotic ribosome ..
  49. pmc Negative regulation of HDM2 to attenuate p53 degradation by ribosomal protein L26
    Ying Zhang
    State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China
    Nucleic Acids Res 38:6544-54. 2010
    ..the cell cycle, apoptosis and tumorigenesis through interacting with other molecules, including several ribosomal proteins. To identify novel HDM2 regulators, we performed a yeast two-hybrid screening using HDM2 as bait...
  50. pmc Elucidation of the assembly events required for the recruitment of Utp20, Imp4 and Bms1 onto nascent pre-ribosomes
    Jorge Pérez-Fernández
    Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer, CSIC University of Salamanca, Campus Unamuno s n, E37007 Salamanca, Spain
    Nucleic Acids Res 39:8105-21. 2011
    ..These results provide new information about the functional relationships among 90S particle components and the events that are required for their stepwise incorporation onto the primary pre-rRNA...
  51. pmc Quality control mechanisms during ribosome maturation
    Katrin Karbstein
    Department of Cancer Biology, The Scripps Research Institute, 130 Scripps Way 2C2, Jupiter, FL 33458, USA
    Trends Cell Biol 23:242-50. 2013
    ..Furthermore, haploinsufficiency of ribosomal proteins, which increases the chances of forming incompletely assembled ribosomes, can predispose to cancer...
  52. pmc The ribosomal protein genes and Minute loci of Drosophila melanogaster
    Steven J Marygold
    Growth Regulation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3PX, UK
    Genome Biol 8:R216. 2007
    Mutations in genes encoding ribosomal proteins (RPs) have been shown to cause an array of cellular and developmental defects in a variety of organisms...
  53. ncbi Introns within ribosomal protein genes regulate the production and function of yeast ribosomes
    Julie Parenteau
    Laboratoire de génomique fonctionnelle de l Université de Sherbrooke, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Quebec, Canada
    Cell 147:320-31. 2011
    In budding yeast, the most abundantly spliced pre-mRNAs encode ribosomal proteins (RPs)...
  54. pmc Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit
    Anke M Mulder
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 330:673-7. 2010
    ..DSP results were integrated with mass spectrometry data to construct the first ribosome-assembly mechanism that incorporates binding dependencies, rate constants, and structural characterization of populated intermediates...
  55. pmc Nonessential plastid-encoded ribosomal proteins in tobacco: a developmental role for plastid translation and implications for reductive genome evolution
    Tobias T Fleischmann
    Max Planck Institut fur Molekulare Pflanzenphysiologie, D 14476 Potsdam Golm, Germany
    Plant Cell 23:3137-55. 2011
    ..translational activity is essential for cell survival in tobacco (Nicotiana tabacum), individual plastid ribosomal proteins can be nonessential...
  56. ncbi The gene encoding ribosomal protein S19 is mutated in Diamond-Blackfan anaemia
    N Draptchinskaia
    Department of Genetics and Pathology, Uppsala University, Sweden
    Nat Genet 21:169-75. 1999
    ..These mutations are associated with clinical features that suggest a function for RPS19 in erythropoiesis and embryogenesis...
  57. pmc Cracking pre-40S ribosomal subunit structure by systematic analyses of RNA-protein cross-linking
    Sander Granneman
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, Scotland, UK
    EMBO J 29:2026-36. 2010
    ..Nob1 binds before pre-rRNA cleavage, and we conclude that structural reorganization is needed to bring together the catalytic PIN domain and its target...
  58. ncbi A Drosophila Minute gene encodes a ribosomal protein
    K Kongsuwan
    Nature 317:555-8. 1985
    ..This activity is specific to the 99D Minute as it does not suppress other Minute loci elsewhere in the genome. This result provides direct evidence that the Minute locus at the 99D interval encodes the ribosomal protein 49...
  59. pmc Ribosomal protein L24 defect in belly spot and tail (Bst), a mouse Minute
    Edward R Oliver
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Development 131:3907-20. 2004
    ..Bacterial artificial chromosome (BAC) and cDNA transgenes correct the mutant phenotypes. Our findings establish Bst as a mouse Minute and provide the first detailed characterization of a mammalian ribosomal protein mutation...
  60. pmc Functional specificity among ribosomal proteins regulates gene expression
    Suzanne Komili
    Department of Systems Biology, Harvard Medical School, Boston, MA 02119, USA
    Cell 131:557-71. 2007
    ..The yeast Saccharomyces cerevisiae contains many duplicated genes encoding ribosomal proteins. Prior studies have suggested that these duplicated proteins are functionally redundant and affect cellular ..
  61. pmc An assembly landscape for the 30S ribosomal subunit
    Megan W T Talkington
    Department of Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 438:628-32. 2005
    ..involving pulse-chase monitored by quantitative mass spectrometry (PC/QMS) to follow the assembly of the 20 ribosomal proteins with 16S ribosomal RNA during formation of the functional particle...
  62. ncbi Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function
    D Chen
    Department of Pharmacology and Toxicology, Division of Clinical Pharmacology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Oncogene 26:5029-37. 2007
    ..The identification of S7 as a novel MDM2-interacting partner contributes to elucidation of the complex regulation of the MDM2-p53 interaction and has implications in cancer prevention and therapy...
  63. pmc Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19754-9. 2010
    ..5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of ..
  64. pmc Many ribosomal protein genes are cancer genes in zebrafish
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 2:E139. 2004
    ..Our findings suggest that many RP genes may act as haploinsufficient tumor suppressors in fish. Many RP genes might also be cancer genes in humans, where their role in tumorigenesis could easily have escaped detection up to now...
  65. ncbi The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome
    Lori A Passmore
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Mol Cell 26:41-50. 2007
    ..Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA...
  66. pmc Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre-rRNA processing
    Aarti Sahasranaman
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
    EMBO J 30:4020-32. 2011
    The precise functions of most of the ∼200 assembly factors and 79 ribosomal proteins required to construct yeast ribosomes in vivo remain largely unexplored...
  67. ncbi An Arabidopsis Minute-like phenotype caused by a semi-dominant mutation in a RIBOSOMAL PROTEIN S5 gene
    D Weijers
    Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
    Development 128:4289-99. 2001
    ....
  68. ncbi Ribosomal proteins promote leaf adaxial identity
    Yao Yao
    National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People s Republic of China
    Development 135:1325-34. 2008
    ..Because these four ribosomal proteins are located in distinct sites in the ribosomal large subunit, we propose that the conserved translational ..
  69. pmc Ribosomal protein L11 mutation in zebrafish leads to haematopoietic and metabolic defects
    Nadia Danilova
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 90095, USA
    Br J Haematol 152:217-28. 2011
    Mutations in ribosomal proteins are associated with a congenital syndrome, Diamond-Blackfan anaemia (DBA), manifested by red blood cell aplasia, developmental abnormalities and increased risk of malignancy...
  70. pmc Ribosomal protein genes RPS10 and RPS26 are commonly mutated in Diamond-Blackfan anemia
    Leana Doherty
    Division of Genetics and Program in Genomics, The Manton Center for Orphan Disease Research, Children s Hospital Boston, Boston, MA 02115, USA
    Am J Hum Genet 86:222-8. 2010
    ....
  71. pmc Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit
    Kai Yin Lo
    Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell 39:196-208. 2010
    ..final maturation of ribosomes occurs in the cytoplasm, where trans-acting factors are removed and critical ribosomal proteins are added for functionality...
  72. ncbi Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins
    Violaine Pinon
    Department of Crop Genetics, John Innes Centre, Norwich NR4 7UH, UK
    Development 135:1315-24. 2008
    ..PGY1, PGY2 and PGY3 encode cytoplasmic large subunit ribosomal proteins, L10a, L9 and L5, respectively...
  73. pmc Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules
    Katharina Merz
    Universitaet Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany
    Genes Dev 22:1190-204. 2008
    ..We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1...
  74. ncbi Synthesis of the translational apparatus is regulated at the translational level
    O Meyuhas
    Department of Biochemistry, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    Eur J Biochem 267:6321-30. 2000
    ..Finally, the possible involvement of pyrimidine-binding proteins in the translational control of TOP mRNAs is discussed...
  75. ncbi The weird and wonderful world of bacterial ribosome regulation
    Daniel N Wilson
    Gene Center and Department of Chemistry and Biochemistry, University of Munich, Munich, Germany
    Crit Rev Biochem Mol Biol 42:187-219. 2007
    ....
  76. pmc Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly
    Tadepalli Adilakshmi
    T C Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218 2685, USA
    Nature 455:1268-72. 2008
    ..Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA...
  77. ncbi The bacterial ribosome as a target for antibiotics
    Jacob Poehlsgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nat Rev Microbiol 3:870-81. 2005
    ..The crystal structures also provide insight as to how existing drugs might be derivatized (or novel drugs created) to improve binding and circumvent resistance...
  78. pmc Ribosomal proteins are targets for the NEDD8 pathway
    Dimitris P Xirodimas
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO Rep 9:280-6. 2008
    ..By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway...
  79. pmc Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits
    Marie Françoise O'Donohue
    Laboratoire de Biologie Moléculaire des Eucaryotes, Université de Toulouse UPS and Centre National de La Recherche Scientifique, F 31000 Toulouse, France
    J Cell Biol 190:853-66. 2010
    Our knowledge of the functions of metazoan ribosomal proteins in ribosome synthesis remains fragmentary...
  80. pmc The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly
    Mengxi Jiang
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109 1048, USA
    J Bacteriol 188:6757-70. 2006
    ..proteomic approach to show that CgtA(E) is required for optimal incorporation of certain late-assembly ribosomal proteins into the large ribosomal subunit...
  81. ncbi A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly
    Takbum Ohn
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA
    Nat Cell Biol 10:1224-31. 2008
    ..receptor for activated C kinase 1), prohibitin-2, glyceraldehyde-3-phosphate dehydrogenase and numerous ribosomal proteins. Our results suggest that O-GlcNAc modification of the translational machinery is required for aggregation ..
  82. pmc Yvh1 is required for a late maturation step in the 60S biogenesis pathway
    Stefan Kemmler
    Institute of Biochemistry, ETH Zurich, CH 8093 Zurich, Switzerland
    J Cell Biol 186:863-80. 2009
    ..Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits...
  83. ncbi Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin
    Joerg M Harms
    Cluster of Excellence for Macromolecular Complexes, Institut für Organische Chemie und Chemische Biologie, J W Goethe Universität Frankfurt am Main, Max von Laue Strasse 7, D 60438 Frankfurt am Main, Germany
    Mol Cell 30:26-38. 2008
    ..The results suggest that L11 acts as a molecular switch to control L7 binding and plays a pivotal role in positioning one L7-CTD monomer on the G' subdomain of EF-G to regulate EF-G turnover during protein synthesis...
  84. ncbi The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit
    D E Brodersen
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 103:1143-54. 2000
    ..For each of these antibiotics, interactions with the 30S subunit suggest a mechanism for its effects on ribosome function...
  85. ncbi The ribosomal stalk binds to translation factors IF2, EF-Tu, EF-G and RF3 via a conserved region of the L12 C-terminal domain
    Magnus Helgstrand
    Department of Biophysical Chemistry, Lund University, Box 124, SE 22100 Lund, Sweden
    J Mol Biol 365:468-79. 2007
    ..These results indicate that the L12-factor complexes will be highly populated on the ribosome, because of the high local concentration of ribosome-bound factor with respect to L12...
  86. ncbi A NusE:NusG complex links transcription and translation
    Björn M Burmann
    Lehrstuhl Biopolymere und Forschungszentrum für Bio Makromoleküle, Universitat Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany
    Science 328:501-4. 2010
    ....
  87. pmc Inside the 40S ribosome assembly machinery
    Katrin Karbstein
    Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
    Curr Opin Chem Biol 15:657-63. 2011
    ..rRNA transcription, modification, folding and cleavage from precursor transcripts, and association of ribosomal proteins (Rps)...
  88. pmc Ribosome biogenesis is sensed at the Start cell cycle checkpoint
    Kara A Bernstein
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA
    Mol Biol Cell 18:953-64. 2007
    ..Thus ribosome biogenesis may be sensed at multiple steps in Start regulation. Ribosome biogenesis may thus both delay Start by increasing the cell size setpoint and independently may promote Start by inactivating Whi5...
  89. ncbi One core, two shells: bacterial and eukaryotic ribosomes
    Sergey Melnikov
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France
    Nat Struct Mol Biol 19:560-7. 2012
    ..Here we describe selected bacteria- or eukaryote-specific structural features of the ribosome and discuss the functional implications of some of them...
  90. pmc The other lives of ribosomal proteins
    Rital B Bhavsar
    Department of Biology, University of Dayton, OH 45469 2320, USA
    Hum Genomics 4:327-44. 2010
    Despite the fact that ribosomal proteins are the constituents of an organelle that is present in every cell, they show a surprising level of regulation, and several of them have also been shown to have other extra-ribosomal functions, ..
  91. ncbi Alveolate phylogeny inferred using concatenated ribosomal proteins
    Tsvetan R Bachvaroff
    Smithsonian Environmental Research Center, 647 Contees Wharf Road, Edgewater, Maryland 21037, USA
    J Eukaryot Microbiol 58:223-33. 2011
    ..and O. marina. Both bootstrap and approximately unbiased test results confirmed that P. marinus, Amoebophrya sp., O. marina, and the remaining dinoflagellates form a monophyletic lineage to the exclusion of Apicomplexa...
  92. pmc Structural insight into nascent polypeptide chain-mediated translational stalling
    Birgit Seidelt
    Gene Center and Center for Integrated Protein Science Munich CIPSM, Department for Chemistry and Biochemistry, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Science 326:1412-5. 2009
    ..We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel...
  93. ncbi Structures of the bacterial ribosome at 3.5 A resolution
    Barbara S Schuwirth
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 310:827-34. 2005
    ....
  94. pmc Crosstalk between c-Myc and ribosome in ribosomal biogenesis and cancer
    Mu Shui Dai
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine and Simon Cancer Center, Indianapolis, Indiana 46202, USA
    J Cell Biochem 105:670-7. 2008
    ..Hence, in this review, we will provide some prospects on the interplay between c-Myc and ribosomal proteins during ribosomal biogenesis and discuss its implications in cancer.
  95. pmc Comparative analysis of processed ribosomal protein pseudogenes in four mammalian genomes
    Suganthi Balasubramanian
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Genome Biol 10:R2. 2009
    ..The availability of genome sequences of numerous organisms allows comparative study of pseudogenes in syntenic regions. Conservation of pseudogenes suggests that they might have a functional role in some instances...
  96. pmc The complete nucleotide sequence and RNA editing content of the mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis of the mitochondrial genomes of rapeseed and Arabidopsis thaliana
    Hirokazu Handa
    Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, Sapporo 062 8555, Japan
    Nucleic Acids Res 31:5907-16. 2003
    ....
  97. pmc Ribosomal protein L5 and L11 mutations are associated with cleft palate and abnormal thumbs in Diamond-Blackfan anemia patients
    Hanna T Gazda
    Division of Genetics and Program in Genomics, The Manton Center for Orphan Disease Research, Children s Hospital Boston, Boston, MA 02115, USA
    Am J Hum Genet 83:769-80. 2008
    ....
  98. ncbi Ribosomal protein L32 of Saccharomyces cerevisiae regulates both splicing and translation of its own transcript
    M D Dabeva
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461
    J Biol Chem 268:19669-74. 1993
    ..In cells overproducing L32-mRNA about half the excess mRNA was found in ribonucleoproteins of < 25 S, unassociated with ribosomal particles. Much of the rest was found in ribonucleoproteins of 80-120 S...
  99. ncbi Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit
    Thorsten Schäfer
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nature 441:651-5. 2006
    ..In vivo depletion of Hrr25 inhibits growth and leads to the accumulation of immature 40S subunits that contain unstably bound Rps3. We conclude that the kinase activity of Hrr25 regulates the maturation of 40S ribosomal subunits...
  100. ncbi Ribosomal protein l19 is a prognostic marker for human prostate cancer
    Alix Bee
    Department of Molecular Pathology, School of Clinical Laboratory Sciences and Royal Liverpool and Broadgreen NHS Trust, University of Liverpool, Liverpool, United Kingdom
    Clin Cancer Res 12:2061-5. 2006
    ..05), revealing RPL19 to be a sensitive predictor of prostate cancer progression. Expression of this protein could be a valuable marker in prostate cancer diagnosis and patient management...
  101. pmc RNA chaperone activity of L1 ribosomal proteins: phylogenetic conservation and splicing inhibition
    Stefan L Ameres
    Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Dr Bohrgasse 9 5, A 1030 Vienna, Austria
    Nucleic Acids Res 35:3752-63. 2007
    ..Mutational analysis of MjaL1 excludes the possibility that the inhibitory effect is due to stronger RNA binding. To our knowledge, MjaL1 is the first example of a protein that inhibits group I intron splicing...

Research Grants70

  1. PRE- AND POSTGRADUATE TRAINING IN MOLECULAR HEMATOLOGY
    J Evan Sadler; Fiscal Year: 2013
    ..Completion of this program will prepare talented trainees for careers in basic and translational hematology research, to make discoveries that will transform the diagnosis and treatment of hematologic diseases. ..
  2. Mechanisms of Splicing Regulation During Environmental Stress in Yeast
    Jaclyn C Greimann; Fiscal Year: 2012
    ..The goal of this research proposal is to understand the mechanisms regulating RNA splicing in response to environmental stress. ..
  3. CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION
    Steven G Clarke; Fiscal Year: 2013
    ..We will determine the role of lysine protein methylation reactions in the translational apparatus, including ribosomal proteins and elongation factors...
  4. Using Mass Spectroscopy to Characterize Transcription Proteins and F2-Isoprostane
    BETTYE HENNINTON; Fiscal Year: 2011
    ..In vivo, the promoters of most genes encoding glycolytic and ribosomal proteins (RP) contain sites occupied by Rap 1p (1)...
  5. Ribosomal Protein Gene Silencing in Neural Crest Stem Cells
    JOHNSON MING YU LIU; Fiscal Year: 2010
    ..To date, all cases of DBA have been linked to mutations in genes encoding ribosomal proteins, however, orofacial anomalies (cleft palate) were found only in patients with RPL5 mutations...
  6. Nuclear Control of Mitochondrial Gene Expression
    Gerald Shadel; Fiscal Year: 2013
    ..Knowledge gained from these studies holds therapeutic promise for mitochondrial disease and age-related pathology based on augmenting mitochondrial gene expression and directly addresses the human disorders obesity and Leigh Syndrome. ..
  7. PHOSPHOPEPTIDE METABOLISM IN ADIPOCYTES
    Joseph Avruch; Fiscal Year: 2013
    ..We will also determine how TOR complex 1 controls the translation of RNAs that bind to the protein IMP2, variants of whose gene appear to confer susceptibility to type 2 diabetes. ..
  8. Identification and characterization of inhibitors for hantavirus replication
    MOHAMMAD AYOUB MIR; Fiscal Year: 2013
    ....
  9. SECOND MESSENGERS IN THE RETINA
    RAJU VS RAJALA; Fiscal Year: 2012
    ..Our long-term goal is to understand these mechanisms as a foundation on which novel drug therapies can be designed to protect retinal function in patients who suffer from retinal degenerations. ..
  10. Functional Genomic Dissection of Refractory Anemia
    BENJAMIN LEVINE EBERT; Fiscal Year: 2013
    ..with a similar phenotype that is also caused by genetic inactivation of one allele of genes encoding ribosomal proteins. We found that haploinsufficiency for miR-145 causes increased expression of a key target gene, FLI-1, ..
  11. Model systems for hematologic disorders caused by ribosomal protein deficiency
    George Thomas; Fiscal Year: 2010
    ..on the p53 response and on rpL5 and rpL11 translation in primary human hematopoietic cells depleted of ribosomal proteins;and Aim 3: Assess the effects of dexamethasone, lenalidomide, and leucine in an in vivo model of ribosomal ..
  12. MODELING AND RESCUE OF DBA IN THE MOUSE
    Philip J Mason; Fiscal Year: 2010
    ..Work from several groups has demonstrated an intriguing connection between ribosomal proteins, specifically RPL5 and RPL11, and the regulation of p53...
  13. Novel mouse models of inflammation-resolution
    Paul L Fox; Fiscal Year: 2010
    ..These models may reveal alternative targets for novel anti-inflammatory therapeutic strategies that, because of their specificity, may exhibit minimal adverse side-effects. ..
  14. Role of the Unfolded Protein Response in Beta Cell
    Randal J Kaufman; Fiscal Year: 2013
    ..Is Atfda and/or Atfdp deletion detrimental to p cells upon I r e l d ''deletion, HFD feeding, or Akita mutation? ..
  15. Human Pluripotent Stem Cell and Progenitor Models of Cardiac and Blood Diseases
    LEONARD IRA ZON; Fiscal Year: 2013
    ..disrupt genomic stability (Fanconi's anemia and Dyskeratosis congenita), specify aberrant nucleolar or ribosomal proteins (Shwachman-Bodian-Diamond Syndrome and Diamond-Blackfan Anemia), and represent a constitutional'trisomy ..
  16. Single Molecule Dynamics of mRNA Translation
    Yale E Goldman; Fiscal Year: 2013
    ..Fluorescent reporter and smFRET pairs are introduced specifically into both aminoacyl tRNAs and ribosomal proteins L1 and L11, which are proximal to the tRNA E-site and A-site, respectively...
  17. Signaling iteractions in cytokinesis, cell wall biogenesis, and growth in fungi
    Jose R Rodriguez-Medina; Fiscal Year: 2011
    ..Knowledge of the interaction between myosin II and stress signaling can lead to identification of potential targets for new antifungal drugs. ..
  18. Identifying and characterizing gene(s) involved in beta-amyloid production.
    Huaxi Xu; Fiscal Year: 2013
    ....
  19. Bacterial effectors targeting the IKK/NF-kB pathway
    PHILIP ROSS HARDWIDGE; Fiscal Year: 2013
    ..modulate innate immunity;2) clarify how pathogens have evolved to co-opt the accessory nuclear functions of ribosomal proteins;and 3) characterize how bacteria have integrated their virulence proteins into host signal transduction ..
  20. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2013
    ..Cellular proteins that are required for retroviral replication are potential targets for the development of new drugs to treat AIDS. ..
  21. Metal Ion Homeostasis in Bacillus subtilis
    John D Helmann; Fiscal Year: 2012
    ..zinc metalloproteins provides a "zinc-sparing response" and the expression of a set of alternative ribosomal proteins displaces small, Zn-containing peptides from the ribosome to provide zinc for the cell...
  22. Mechanism of selenoprotein synthesis
    Marla Berry; Fiscal Year: 2009
    ..Through these studies, we hope to gain insights into the mechanisms by which organisms have evolved to maximize utilization of this essential trace element in development and disease prevention. ..
  23. Bacterial effectors targeting the IKK/NF-kB pathway
    PHILIP ROSS HARDWIDGE; Fiscal Year: 2012
    ..modulates innate immunity;2) clarify how pathogens have evolved to co-opt the accessory nuclear functions of ribosomal proteins;and 3) characterize how bacteria have integrated their virulence proteins into subverting host signal ..
  24. Role of Ribosomal Proteins in Vascular Smooth Muscle Cell Proliferation
    Elaine M Smolock; Fiscal Year: 2010
    ..Two of the candidate genes identified were ribosomal proteins (Rpl), Rpl17 and Rpl7-like1...
  25. ROLE OF ACA11, AN ORPHAN SMALL NUCLEOLAR RNA IN RESISTANCE TO CANCER CHEMOTHERAPY
    Jason Weber; Fiscal Year: 2013
    ..regulates ROS levels through modulating splicing of snoRNAs from ribosomal protein transcripts Aim 3: To test the hypothesis that overexpression of ACA11 leads to an inhibition of nucleolar stress-induced, p53-regulated ROS production ..
  26. Translational Recoding of UGA as Selenocysteine in Selenoprotein Synthesis
    Donna M Driscoll; Fiscal Year: 2013
    ....
  27. Disruption of NF-kB signaling by diarrheagenic Escherichia coli
    PHILIP ROSS HARDWIDGE; Fiscal Year: 2010
    ..The results from these studies are likely to suggest new therapeutic strategies for diarrheal pathogens. ..
  28. The role of ribosomal proteins in the p53-MDM2 pathway
    Hua Lu; Fiscal Year: 2013
    ..supported by my previously funded R01 and by many other groups has now firmly established the role of ribosomal proteins (RP), such as RPL11, RPL5, and RPL23, in p53 response to various cellular and environmental carcinogens or ..
  29. Poly(ADP-ribose)Polymerase 1 Regulates Nucleolar Structure and Functions
    ERNEST KOJO BOAMAH; Fiscal Year: 2012
    ..We will examine the incorporation of fluorescence tagged RpL22 and RpL23 ribosomal proteins into the translation machinery in flies with compromised PARP1 activity using polysome gradient, ..
  30. The Function of rpL5 and rpL11 in induction of p53
    George Thomas; Fiscal Year: 2013
    ..abstract_text> ..
  31. Translational Silencing in Monocytes: Role of L13a
    Barsanjit Mazumder; Fiscal Year: 2013
    ..We believe these studies will discover novel cellular targets for new generation of anti-inflammatory small molecules against atherosclerosis and other inflammatory diseases. ..
  32. Regulation of Neonatal Muscle Protein Synthesis
    Teresa A Davis; Fiscal Year: 2012
    ....
  33. RiboTag: A novel technique to profile cell type specific gene expression and inv
    GEORGE STANLEY MCKNIGHT; Fiscal Year: 2013
    ..This proposal addresses that need with a novel biochemical and genetic technique to express epitope-tagged ribosomal proteins in response to a cell type specific Cre recombinase in mouse brain...
  34. New Gene Discoveries and Biology of Ribosomes in Diamond-Blackfan Anemia
    HANNA TERESA GAZDA; Fiscal Year: 2013
    ..Specific Aim 2. Determine the role of DBA genes in pre-RNA maturation. Specific Aim 3. Identify modifier genes in DBA by performing a genome-wide association study. ..
  35. New gene discoveries and biology of ribosomes in Diamond-Blackfan anemia.
    HANNA TERESA GAZDA; Fiscal Year: 2013
    ..Specific Aim 2. Determine the role of DBA genes in pre-RNA maturation. Specific Aim 3. Identify modifier genes in DBA by performing a genome-wide association study. ..
  36. mTOR in Growth and Protection of Hypertrophying Myocardium
    Dhandapani Kuppuswamy; Fiscal Year: 2012
    ..These studies are envisioned to open new avenues of research into how the mTOR complexes influence hypertrophic growth and identify novel cardio-therapeutic strategies for rapamycin. ..
  37. Alcohol Consumption and Risk of NHL: Role of MTOR Dysfunction
    Ronald B Gartenhaus; Fiscal Year: 2013
    ..abstract_text> ..
  38. Biochemistry of lysyl-tRNA synthetases
    Michael Ibba; Fiscal Year: 2013
    ....
  39. Delivery of a bacterial inhibitor of NF-kB to colon tumors
    PHILIP ROSS HARDWIDGE; Fiscal Year: 2012
    ....
  40. Specialized ribosomes in control of gene expression and embryonic development
    Maria Barna; Fiscal Year: 2011
    ..Our studies will delineate how the 79 distinct ribosomal proteins (RPs) that associate with ribosomal RNA confer greater specificity to the RNA-based translational machinery ..
  41. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    STEPHEN PAINE GOFF; Fiscal Year: 2013
    ..t Description, ..
  42. Novel technology for analysis of cardiac &BMP-specific gene expression profiling
    TODD ANDREW TOWNSEND; Fiscal Year: 2013
    ..which Biotin Ligase Recognition Peptide (BLRP) epitope is attached to accessible regions of two independent ribosomal proteins (Rpls), Rpl18a and Rpl23 and the second component drives expression of BirA in a lineage or cell-signaling ..
  43. Pyrazinamide and Trans-Translation in Mycobacterium tuberculosis
    Ying Zhang; Fiscal Year: 2013
    ..These studies will provide useful information for design of new and more powerful drugs that target persisters for shortening the duration of TB treatment. ..
  44. Translational profiling of somatosensory afferent neurons
    David D McKemy; Fiscal Year: 2013
    ..abstract_text> ..
  45. A Vital Tool for the Study of DBA:The Diamond Blackfan Anemia Registry
    Jeffrey M Lipton; Fiscal Year: 2013
    ..The actuarial cancer risk although increased, is as of yet, undetermined. To date six genes encoding ribosomal proteins, of both the small and large subunits, have been found to be mutated, representing nearly 50% of patients ..
  46. BIOSYNTHESIS OF RNAS
    Christine Guthrie; Fiscal Year: 2013
    ..Alterations in the dynamics of transient targeting generate a bi-modal population of cells, explaining the incomplete penetrance of the brr6-1 export phenotype. ..
  47. Role of Rpl22 in ER Stress and Colitis
    David L Wiest; Fiscal Year: 2013
    ..Through these efforts, we expect to gain substantial insight into how Rpl22 may serve as an integrator that links defects in cellular stress responses to inflammation in the gut. ..
  48. Cell-specific translational profiling in Huntington's Disease mouse models
    ROBERT JONATHAN FENSTER; Fiscal Year: 2012
    ..This technology uses GFP-tagged ribosomal proteins to isolate translated mRNA pools from genetically defined cell populations, eliminating the need to ..
  49. The role of deregulated protein synthesis control in Myc-induced tumorigenesis
    Davide Ruggero; Fiscal Year: 2013
    ..rates by controlling the expression of multiple components of the protein synthetic machinery, including ribosomal proteins, initiation factors of translation, Pol III and rDNA...
  50. Molecular Pathogenesis of Diamond Blackfan Anemia
    KATHLEEN MIHO SAKAMOTO; Fiscal Year: 2013
    ..Therefore, results from this work will improve the quality of life of DBA patients. ..
  51. Investigating the Molecular Bases of Class-Specific Dendrite Morphogenesis
    Daniel N Cox; Fiscal Year: 2012
    ..BED- type protein encoded by CG3995 functions as a downstream efector of Cut and functionally interacts with ribosomal proteins to direct cell-type specific dendrite morphogenesis;and 3) that the evolutionarily conserved Hox proteins, ..
  52. Thiazolyl peptide analogs that provide insight into antibiotic targeting
    TRAVIS SCOTT YOUNG; Fiscal Year: 2012
    ..This understanding is vital to the development of new therapeutics to combat emerging bacterial resistance. ..
  53. The Architecture and Function of RNPs Required for Ribosome Biogenesis
    Susan J Baserga; Fiscal Year: 2013
    ..the cellular powerhouses of translation, are assembled from rRNA, the catalytic moiety, and scores of ribosomal proteins. In eukaryotic cells, the pre-rRNA undergoes extensive chemical modifications and several cleavage events as ..
  54. Role of the SrmB Helicase in Ribosome Assembly investigated by Mass Spectrometry
    Carla F Cervantes; Fiscal Year: 2013
    ..Third, we will investigate the relationship between SrmB and other ribosomal proteins by perturbing ribosomal protein expression by overexpression in ?SrmB E...
  55. MECHANISM, ACTIVATION, AND CONTROL OF RRNA TRANSCRIPTION
    Richard L Gourse; Fiscal Year: 2013
    The machinery responsible for making proteins (e.g. ribosomal RNA, ribosomal proteins, translation factors, and tRNAs) is central to growth and development of all organisms...
  56. Mechanism of Compensatory Renal Hypertrophy
    Jian Kang Chen; Fiscal Year: 2013
    ..We propose to use combined in vivo and in vitro studies to investigate both upstream and downstream of mTOR activation in CRH. ..
  57. Mechanisms of S6K1 Mediated Insulin Resistance
    George Thomas; Fiscal Year: 2010
    ..Thus, if S6K1 is a critical player in these diseases the development of a small molecule inhibitor to S6K1 may act to improve the quality of life of those suffering from insulin resistance. ..
  58. Mass Spectrometry of Ribosomal RNA:Protein Interactions
    PATRICK LIMBACH; Fiscal Year: 2009
    ..Those developments will be used to identify specific recognition elements of the RNAs and ribosomal proteins involved in the initiation step in protein synthesis...
  59. Mass Spectrometry of Ribosomal RNA:Protein Interactions
    Patrick A Limbach; Fiscal Year: 2013
    ..Those developments will be used to identify specific recognition elements of the RNAs and ribosomal proteins involved in the initiation step in protein synthesis...
  60. Metabolic Adaptive Responses in Cancer
    David R Plas; Fiscal Year: 2013
    ....
  61. Assembling an understanding of Ribosome Biogenesis
    Gloria M Culver; Fiscal Year: 2013
    ..First, conformational changes within 16S rRNA and specific rRNA nucleotides and ribosomal proteins that facilitate these changes during different stages of assembly in vitro and in vivo will be identified...
  62. Dysregulated microRNA function in diamond blackfan anemia
    Carl D Novina; Fiscal Year: 2013
    ..Our studies will establish novel connections between RPG expression, miRNA function, miRNA-targeted gene expression, and erythropoiesis, and may reveal new targets for DBA therapy. ..
  63. Nanoliter Lab-on-a-chip for Protein Crystallization
    Vamsee Pamula; Fiscal Year: 2007
    ..Crystal structures of proteins such as enzymes, ribosomal proteins, bacterial toxins, hormones, and receptors are essential to understand important physiological processes and ..
  64. Role of Phospholipase C in Transcriptional Regulation
    Ales Vancura; Fiscal Year: 2009
    ..Elucidation of the regulatory interactions between PLC signaling pathway and transcriptional machinery is important for the identification of novel molecular targets for cancer therapy. ..
  65. Identification of the ribosomal target of Shiga-like toxins
    NILGUN TUMER; Fiscal Year: 2007
    ..Our studies established for the first time that binding of RIPs to ribosomal proteins is essential for depurination of ribosomes...
  66. MULTIPLE CHIMERIC TRANSCRIPTS IN THE T(3--21)
    Giuseppina Nucifora; Fiscal Year: 1999
    ....
  67. MOUSE MINUTES: A GENETIC STUDY OF RIBOSOMAL PROTEINS
    Thomas Glaser; Fiscal Year: 2007
    ....
  68. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 2004
    ..These studies provide cloned genes, molecular approaches, and characterization of regulatory processes that can potentially be manipulated to interfere with disease transmission by blood-feeding arthropods. ..
  69. Ribosome Biogenesis in Diamond Blackfan Anemia
    Monica Bessler; Fiscal Year: 2007
    ....
  70. Ribosomes of Plasmodium falciparum
    JEAN FEAGIN; Fiscal Year: 2006
    ..Our data may therefore produce information useful to development of new anti-malarial treatments. ..