recombinant fusion proteins

Summary

Summary: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

Top Publications

  1. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
  2. pmc Targeting DNA double-strand breaks with TAL effector nucleases
    Michelle Christian
    Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, USA
    Genetics 186:757-61. 2010
  3. ncbi Global analysis of protein expression in yeast
    Sina Ghaemmaghami
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94143 2240, USA
    Nature 425:737-41. 2003
  4. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
  5. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
  6. ncbi Global analysis of protein localization in budding yeast
    Won Ki Huh
    Howard Hughes Medical Institute, University of California San Francisco, Department of Biochemistry and Biophysics, 600 16th Street, San Francisco, California 94143 2240, USA
    Nature 425:686-91. 2003
  7. ncbi Gateway-compatible vectors for plant functional genomics and proteomics
    Keith W Earley
    Biology Department, Washington University, 1 Brookings Drive, St Louis, MO 63130, USA
    Plant J 45:616-29. 2006
  8. ncbi Engineering and characterization of a superfolder green fluorescent protein
    Jean Denis Pédelacq
    Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Nat Biotechnol 24:79-88. 2006
  9. ncbi Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
    Tsuyoshi Nakagawa
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 1060 Nishikawatsu, Matsue, Japan
    J Biosci Bioeng 104:34-41. 2007
  10. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002

Research Grants

Detail Information

Publications276 found, 100 shown here

  1. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
    ..Thus, these plasmids should further facilitate the rapid analysis of gene function in S. cerevisiae...
  2. pmc Targeting DNA double-strand breaks with TAL effector nucleases
    Michelle Christian
    Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, USA
    Genetics 186:757-61. 2010
    ..Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites...
  3. ncbi Global analysis of protein expression in yeast
    Sina Ghaemmaghami
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94143 2240, USA
    Nature 425:737-41. 2003
    ..Many of these molecules, including essential proteins and most transcription factors, are present at levels that are not readily detectable by other proteomic techniques nor predictable by mRNA levels or codon bias measurements...
  4. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
    ..This method will be of general utility for analysis of the autophagosome maturation process...
  5. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
    ..Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes...
  6. ncbi Global analysis of protein localization in budding yeast
    Won Ki Huh
    Howard Hughes Medical Institute, University of California San Francisco, Department of Biochemistry and Biophysics, 600 16th Street, San Francisco, California 94143 2240, USA
    Nature 425:686-91. 2003
    ....
  7. ncbi Gateway-compatible vectors for plant functional genomics and proteomics
    Keith W Earley
    Biology Department, Washington University, 1 Brookings Drive, St Louis, MO 63130, USA
    Plant J 45:616-29. 2006
    ..Antibodies detecting the FLAG, HA, cMyc and AcV5 tags show relatively little cross-reaction with endogenous proteins in a variety of monocotyledonous and dicotyledonous plants, suggesting broad utility for the tags and vectors...
  8. ncbi Engineering and characterization of a superfolder green fluorescent protein
    Jean Denis Pédelacq
    Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Nat Biotechnol 24:79-88. 2006
    ..X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP...
  9. ncbi Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
    Tsuyoshi Nakagawa
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 1060 Nishikawatsu, Matsue, Japan
    J Biosci Bioeng 104:34-41. 2007
    ..Moreover, tissue-specific and inducible gene expression using a promoter was also monitored with pGWBs. It is expected that, the pGWB system will serve as a powerful tool for plasmid construction in plant research...
  10. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  11. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
    ..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system...
  12. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
    ..Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis...
  13. pmc TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain
    Ting Li
    Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
    Nucleic Acids Res 39:359-72. 2011
    ..Our results demonstrate the feasibility of creating a tool box of novel TALNs with potential for targeted genome modification in organisms lacking facile mechanisms for targeted gene knockout and homologous recombination...
  14. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
    ..Proteins homologous to Scc1p exist in a variety of eukaryotic organisms including humans. A common cohesion apparatus might be used by all eukaryotic cells during both mitosis and meiosis...
  15. ncbi Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
    ..In conclusion, our results showed a role for Rab7 in the final maturation of late autophagic vacuoles...
  16. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..Thus FRET between GFP mutants can monitor localized Ca2+ signals and protein heterodimerization in individual live cells...
  17. ncbi A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins
    Ulrich Rothbauer
    Munich Center for Integrated Protein Science, CiPS M and Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    Mol Cell Proteomics 7:282-9. 2008
    ..This versatile GFP-nanotrap enables a unique combination of microscopic, biochemical, and functional analyses with one and the same protein...
  18. ncbi FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly
    Donna J Webb
    Department of Cell Biology, UVA School of Medicine, P O Box 800732, Charlottesville, Virginia 22908 0732, USA
    Nat Cell Biol 6:154-61. 2004
    ....
  19. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  20. pmc Circularly permuted green fluorescent proteins engineered to sense Ca2+
    T Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, Institute of Physical and Chemical Research (RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
    Proc Natl Acad Sci U S A 98:3197-202. 2001
    ..The Ca(2+)-dependent interaction between calmodulin and M13 in HeLa cells was monitored by the association of the two halves of GFP, neither of which was fluorescent by itself...
  21. ncbi Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse
    Shigemi Hayashi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Dev Biol 244:305-18. 2002
    ..This inducible Cre system will be a broadly useful tool to modulate gene activity in mouse embryos, adults, and culture systems where temporal control is an important consideration...
  22. ncbi Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'
    M L Donnelly
    Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    J Gen Virol 82:1013-25. 2001
    ..This process produces a ribosomal 'skip' from one codon to the next without the formation of a peptide bond...
  23. ncbi Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells
    O Tetsu
    University of California, San Francisco, School of Medicine, Cancer Research Institute, 94143 0128, USA
    Nature 398:422-6. 1999
    ..Abnormal levels of beta-catenin may therefore contribute to neoplastic transformation by causing accumulation of cyclin D1...
  24. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  25. ncbi Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
    L Timmons
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Gene 263:103-12. 2001
    ....
  26. pmc Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution
    J Lippincott
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 143:1947-60. 1998
    ..Overexpression of Cyk2p also blocks cytokinesis, most likely due to a loss of the septins from the bud neck, indicating that Cyk2p may also play a role in regulating the localization of the septins...
  27. ncbi GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function
    Daniel M Rosenbaum
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 318:1266-73. 2007
    ..Amino acids known to regulate receptor function are linked through packing interactions and a network of hydrogen bonds, suggesting a conformational pathway from the ligand-binding pocket to regions that interact with G proteins...
  28. ncbi Enhancing gene targeting with designed zinc finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
    Science 300:764. 2003
  29. pmc A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules
    Laura A Banaszynski
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Cell 126:995-1004. 2006
    ..This general strategy for regulating protein stability should enable conditional perturbation of specific proteins with unprecedented control in a variety of experimental settings...
  30. pmc Visualization of the intracellular behavior of HIV in living cells
    David McDonald
    Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Cell Biol 159:441-52. 2002
    ..Based on these observations, we propose that HIV uses dynein and the microtubule network to facilitate the delivery of the viral genome to the nucleus of the cell during early postentry steps of the HIV life cycle...
  31. pmc Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
    B Grant
    Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
    Mol Biol Cell 10:4311-26. 1999
    ..We describe a new member of the low-density lipoprotein receptor superfamily, RME-2, identified in our screens for endocytosis defective mutants. We show that RME-2 is the C. elegans yolk receptor...
  32. ncbi Yeast surface display for screening combinatorial polypeptide libraries
    E T Boder
    Department of Chemical Engineering, University of Illinois, Urbana 61801, USA
    Nat Biotechnol 15:553-7. 1997
    ..Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands...
  33. ncbi Osteoprotegerin: a novel secreted protein involved in the regulation of bone density
    W S Simonet
    Department of Molecular Genetics, Amgen Inc, Thousand Oaks, California 91320, USA
    Cell 89:309-19. 1997
    ..These data show that OPG can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity...
  34. pmc Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface
    Julie P I Welburn
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Mol Cell 38:383-92. 2010
    ..In total, rather than generating exclusively binary changes in microtubule binding, our results suggest a mechanism for the tension-dependent fine-tuning of kinetochore-microtubule interactions...
  35. ncbi Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses
    R Rizzuto
    Department of Biomedical Sciences and the National Research Council Center for the Study of Biomembranes, University of Padova, Via Colombo 3, 35121 Padova, Italy
    Science 280:1763-6. 1998
    ..These results emphasize the importance of cell architecture and the distribution of organelles in regulation of Ca2+ signaling...
  36. ncbi Efficient and rapid induction of a chronic myelogenous leukemia-like myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow
    W S Pear
    Department of Pathology and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA
    Blood 92:3780-92. 1998
    ..These results demonstrate that murine CML recapitulates important features of human CML. As such, it should be an excellent model for addressing specific issues relating to the pathogenesis and treatment of this disease...
  37. pmc A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
    Nick S Berrow
    The Oxford Protein Production Facility, Henry Wellcome Building for Genomic Medicine, University of Oxford, Oxford, UK
    Nucleic Acids Res 35:e45. 2007
    ..g. Sf9 cells). These high-throughput screening activities have prompted the development and validation of automated protocols for transfection of mammalian cells and Ni-NTA protein purification...
  38. pmc Movement of Bax from the cytosol to mitochondria during apoptosis
    K G Wolter
    Biochemistry Section, Surgical Neurology Branch, Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 139:1281-92. 1997
    ....
  39. pmc Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 141:1539-50. 1998
    ....
  40. pmc Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases
    Jing Guo
    The Skaggs Institute for Chemical Biology and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Mol Biol 400:96-107. 2010
    ..This enhanced and highly efficient cleavage domain will aid in a variety of ZFN applications in medicine and biology...
  41. ncbi Chimeric nucleases stimulate gene targeting in human cells
    Matthew H Porteus
    Department of Biology, California Institute of Technology, Pasadena CA 91125, USA
    Science 300:763. 2003
  42. ncbi Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease
    Claudine Kraft
    Institute of Biochemistry, HPM, ETH Honggerberg, 8093 Zurich, Switzerland
    Nat Cell Biol 10:602-10. 2008
    ..Taken together, our results suggest a link between ubiquitination and the regulated degradation of mature ribosomes by autophagy...
  43. ncbi Binding of hepatitis C virus to CD81
    P Pileri
    IRIS, Chiron, Siena 53100, Italy
    Science 282:938-41. 1998
    ..Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro...
  44. ncbi Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran
    Ho Min Kim
    Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejon, Korea 305 701
    Cell 130:906-17. 2007
    ..The interaction with Eritoran is mediated by a hydrophobic internal pocket in MD-2. Based on structural analysis and mutagenesis experiments on MD-2 and TLR4, we propose a model of TLR4-MD-2 dimerization induced by LPS...
  45. ncbi From silencing to gene expression: real-time analysis in single cells
    Susan M Janicki
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 116:683-98. 2004
    ..Using this system, we are able to correlate changes in chromatin structure with the progression of transcriptional activation allowing us to obtain a real-time integrative view of gene expression...
  46. ncbi Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis
    Marzena Bienko
    Institute for Biochemistry II, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    Science 310:1821-4. 2005
    ..Our results indicate that Ub-binding domains of Y-family polymerases play crucial regulatory roles in TLS...
  47. pmc Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system
    M Maduro
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Genetics 141:977-88. 1995
    ..Expression of an unc-119/lacZ fusion in transgenic animals is seen in many neurons, suggesting that the unc-119 mutant phenotype is due to a defect in the nervous system...
  48. pmc De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks
    Magdy M Mahfouz
    Center for Plant Stress Genomics and Technology, King Abdullah University of Science and Technology, Thuwal 23955 6900, Kingdom of Saudi Arabia
    Proc Natl Acad Sci U S A 108:2623-8. 2011
    ..Taken together, our data show the feasibility of engineering TALE-based hybrid nucleases capable of generating site-specific DSBs and the great potential for site-specific genome modification in plants and eukaryotes in general...
  49. ncbi Rnq1: an epigenetic modifier of protein function in yeast
    N Sondheimer
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637, USA
    Mol Cell 5:163-72. 2000
    ..These findings identity Rnq1 as a prion, demonstrate that prion domains are modular and transferable, and establish a paradigm for identifying and characterizing novel prions...
  50. ncbi Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2
    M Trommsdorff
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235 9046, USA
    Cell 97:689-701. 1999
    ..These findings suggest that VLDLR and ApoER2 participate in transmitting the extracellular Reelin signal to intracellular signaling processes initiated by mDab1...
  51. ncbi EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ....
  52. pmc The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin
    V Vasioukhin
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 96:8551-6. 1999
    ..These tools now pave the way for testing the functional role of different somatic mutations that may exist in mosaic disorders of the skin, including squamous and basal cell carcinomas...
  53. ncbi Randomized, double-blind, placebo-controlled phase II study of AMG 386 combined with weekly paclitaxel in patients with recurrent ovarian cancer
    Beth Y Karlan
    Cedars Sinai Medical Center, Los Angeles, CA 90048, USA
    J Clin Oncol 30:362-71. 2012
    ....
  54. pmc A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae
    V Guacci
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Cell 91:47-57. 1997
    ..These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link...
  55. ncbi Optimization of membrane protein overexpression and purification using GFP fusions
    David Drew
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Nat Methods 3:303-13. 2006
  56. ncbi Endocytosis by random initiation and stabilization of clathrin-coated pits
    Marcelo Ehrlich
    Department of Cell Biology, Harvard Medical School and the CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 118:591-605. 2004
    ..Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture...
  57. ncbi Membrane phosphatidylserine regulates surface charge and protein localization
    Tony Yeung
    Division of Cell Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada
    Science 319:210-3. 2008
    ..More strongly cationic proteins, normally associated with the plasma membrane, relocalized to endocytic compartments when the plasma membrane surface charge decreased on calcium influx...
  58. ncbi Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method
    Christopher J Tonkin
    Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Parkville, VIC 3010, Australia
    Mol Biochem Parasitol 137:13-21. 2004
    ..falciparum proteins...
  59. pmc ATP-dependent chromatin remodeling by the Cockayne syndrome B DNA repair-transcription-coupling factor
    E Citterio
    Medical Genetic Center, Department of Cell Biology and Genetics, Center for Biomedical Genetics, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands
    Mol Cell Biol 20:7643-53. 2000
    ..CSB is the first repair protein found to play a direct role in modulating nucleosome structure. The relevance of this finding to the interplay between transcription and repair is discussed...
  60. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
    ..Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage...
  61. ncbi Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
    Tatiana Stepanova
    Medical Genetics Center Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands
    J Neurosci 23:2655-64. 2003
    ..We propose that similar mechanisms underlie the specific association of CLIPs and EB1-related proteins with the ends of growing microtubules in non-neuronal and neuronal cells...
  62. pmc Role of polo kinase and Mid1p in determining the site of cell division in fission yeast
    J Bahler
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 143:1603-16. 1998
    ..Given its previously known functions in mitosis and the timing of cytokinesis, Plo1p is thus implicated as a key molecule in the spatial and temporal coordination of cytokinesis with mitosis...
  63. pmc Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis
    Silke Robatzek
    Zurich Basel Plant Science Center, Botanical Institute, University of Basel, 4056 Basel, Switzerland
    Genes Dev 20:537-42. 2006
    ..We propose that plant cells regulate pathogen-associated molecular pattern (PAMP)-mediated PRR activities by subcellular compartmentalization...
  64. ncbi A general method for the covalent labeling of fusion proteins with small molecules in vivo
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Nat Biotechnol 21:86-9. 2003
    ..Here we describe a general method for the covalent labeling of fusion proteins in vivo that complements existing methods for noncovalent labeling of proteins and that may open up new ways of studying proteins in living cells...
  65. pmc Case report of a serious adverse event following the administration of T cells transduced with a chimeric antigen receptor recognizing ERBB2
    Richard A Morgan
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Ther 18:843-51. 2010
    ..We speculate that the large number of administered cells localized to the lung immediately following infusion and were triggered to release cytokine by the recognition of low levels of ERBB2 on lung epithelial cells...
  66. pmc Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome
    Xiaokun Shu
    Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Science 324:804-7. 2009
    ..Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering...
  67. pmc The relationship between force and focal complex development
    Catherine G Galbraith
    Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 159:695-705. 2002
    ....
  68. pmc The threshold for polyglutamine-expansion protein aggregation and cellular toxicity is dynamic and influenced by aging in Caenorhabditis elegans
    James F Morley
    Department of Biochemistry, Molecular Biology, and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 99:10417-22. 2002
    ....
  69. ncbi Counting cytokinesis proteins globally and locally in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 310:310-4. 2005
    ..Proteins concentrated up to 100 times in contractile rings and 7500 times in spindle pole bodies at certain times in the cell cycle. This approach can be used to measure the global and local concentrations of any fusion protein...
  70. ncbi Immunocytokines: a novel class of potent armed antibodies
    Nadine Pasche
    Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Zurich, Switzerland
    Drug Discov Today 17:583-90. 2012
    ....
  71. ncbi Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix
    O Ibraghimov-Beskrovnaya
    Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242
    Nature 355:696-702. 1992
    ....
  72. ncbi Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis
    Pankaj Dhonukshe
    Center for Plant Molecular Biology ZMBP, Auf der Morgenstelle 3, University of Tubingen, D 72076 Tubingen, Germany
    Curr Biol 17:520-7. 2007
    ..Our data demonstrate that clathrin-dependent endocytosis is operational in plants and constitutes the predominant pathway for the internalization of numerous plasma-membrane-resident proteins including PIN auxin efflux carriers...
  73. pmc Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
    Yun Wah Lam
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Biol 17:749-60. 2007
    ....
  74. pmc Mapping the Arabidopsis organelle proteome
    Tom P J Dunkley
    Department of Biochemistry, University of Cambridge, Downing Site, Cambridge CB2 1QW, United Kingdom
    Proc Natl Acad Sci U S A 103:6518-23. 2006
    ..Consequently, genuine organelle residents have been distinguished from contaminating proteins and proteins in transit through the secretory pathway...
  75. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001
    ..Thus, despite the common essential feature of the CAAX (prenylation, AAX tripeptide proteolysis, and carboxyl methylation) motif, the subcellular localizations of Rho GTPases, like their functions, are diverse and dynamic...
  76. pmc Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
    Hiromi Imamura
    Precursory Research for Embryonic Science, Japan Science and Technology Agency, 5 Sanbancho, Chiyoda ku, Tokyo 102 0075, Japan
    Proc Natl Acad Sci U S A 106:15651-6. 2009
    ..This was also confirmed by an experiment using oligomycin A, an inhibitor of F(o)F(1)-ATP synthase. In addition, it was demonstrated that HeLa cells change ATP-generating pathway in response to changes of nutrition in the environment...
  77. pmc Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
    R Gary
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Cell 6:1061-75. 1995
    ..The conformationally inaccessible C-terminal region included the F-actin binding site, suggesting that this activity is likewise regulated by masking...
  78. ncbi Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor
    A Brunet
    Children s Hospital and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 96:857-68. 1999
    ..Within the nucleus, FKHRL1 triggers apoptosis most likely by inducing the expression of genes that are critical for cell death, such as the Fas ligand gene...
  79. ncbi A toxic monomeric conformer of the polyglutamine protein
    Yoshitaka Nagai
    Division of Clinical Genetics, Department of Medical Genetics, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Nat Struct Mol Biol 14:332-40. 2007
    ..We conclude that the toxic conformational transition, and not simply the aggregation process itself, is a therapeutic target for polyQ diseases and possibly for conformational diseases in general...
  80. pmc Monomeric site-specific nucleases for genome editing
    Benjamin P Kleinstiver
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada N6A 5C1
    Proc Natl Acad Sci U S A 109:8061-6. 2012
    ....
  81. pmc Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme
    Jianbin Wang
    Sangamo Biosciences Inc, Richmond, California 94804, USA
    Genome Res 22:1316-26. 2012
    ..Moreover, the potential for a SSB to direct repair pathway choice (i.e., HDR but not NHEJ) may prove advantageous for certain therapeutic applications such as the targeted correction of human disease-causing mutations...
  82. ncbi Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes
    Robert Köchl
    Cancer Research UK, London Research Institute, Secretory Pathways Laboratory, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Traffic 7:129-45. 2006
    ..Stimulation of autophagosome formation by vinblastine was independent of nutrients and mTOR activity but was inhibited by depletion of the Autophagy proteins Atg5 and Atg6, known to be required for autophagy...
  83. ncbi A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux
    Jiri Friml
    Developmental Genetics, Center for Molecular Biology of Plants, University Tübingen, Auf der Morgenstelle 3, D 72076 Tubingen, Germany
    Science 306:862-5. 2004
    ..Our results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes...
  84. ncbi Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis
    H Chen
    Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Nature 394:793-7. 1998
    ..We propose that epsin may participate, together with Eps15, in the molecular rearrangement of the clathrin coats that are required for coated-pit invagination and vesicle fission...
  85. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
    ..When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules...
  86. pmc Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models
    Antony A Cooper
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Science 313:324-8. 2006
    ..Thus, synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable...
  87. ncbi The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family
    G Mosialos
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115
    Cell 80:389-99. 1995
    ..The interaction of LMP1 with these TNFR family-associated proteins is further evidence for their role in signaling and links LMP1-mediated transformation to signal transduction from the TNFR family...
  88. ncbi Targeted delivery of tumor antigens to activated dendritic cells via CD11c molecules induces potent antitumor immunity in mice
    Huafeng Wei
    International Joint Cancer Institute and Shanghai Key Laboratory of Cell Engineering and Antibody, The Second Military Medical Universit, Shanghai, People s Republic of China
    Clin Cancer Res 15:4612-21. 2009
    ....
  89. ncbi PEX11 family members are membrane elongation factors that coordinate peroxisome proliferation and maintenance
    Johannes Koch
    Max F Perutz Laboratories, Center of Molecular Biology, University of Vienna, Department of Biochemistry and Cell Biology, Dr Bohr Gasse 9, 1030, Vienna, Austria
    J Cell Sci 123:3389-400. 2010
    ..Hence, we propose a model for a conserved role of PEX11 proteins in peroxisome maintenance through peroxisome polarization, membrane elongation and segregation...
  90. ncbi Impairment of the ubiquitin-proteasome system by protein aggregation
    N F Bence
    Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA
    Science 292:1552-5. 2001
    ....
  91. pmc Redirecting T-cell specificity by introducing a tumor-specific chimeric antigen receptor
    Bipulendu Jena
    Division of Pediatrics, University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Blood 116:1035-44. 2010
    ..The next-generation trials will deliver designer T cells with improved homing, CAR-mediated signaling, and replicative potential, as investigators move from the bedside to the bench and back again...
  92. ncbi Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus
    Qian Zhang
    Genelux Corporation, San Diego Science Center, San Diego, California 92109, USA
    Cancer Res 67:10038-46. 2007
    ..These findings suggest that immune activation may combine with viral oncolysis to induce tumor eradication in this model, providing a novel perspective for the design of oncolytic viral therapies for human cancers...
  93. pmc Antibody-based therapeutics to watch in 2011
    Janice M Reichert
    Tufts Center for the Study of Drug Development, Boston, MA, USA
    MAbs 3:76-99. 2011
    ..Current as of September 2010, the information presented here will serve as a baseline against which future progress in the development of antibody-based therapeutics can be measured...
  94. ncbi A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes
    Hiroki Nagai
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 295:679-82. 2002
    ..pneumophila. Translocation of RalF protein through the phagosomal membrane is a dot/icm-dependent process. Thus, RalF is a substrate of the Dot/Icm secretion apparatus...
  95. ncbi Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors
    Goshi Ishihara
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Protein Expr Purif 41:27-37. 2005
    ..This cell-free translation system (about 1mg/ml of reaction volume for 6-8 h) has biophysical and biochemical advantages for the synthesis of integral membrane proteins...
  96. ncbi Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation
    D L Lacey
    Department of Pathology, Amgen, Inc, Thousand Oaks, California 91320 1789, USA
    Cell 93:165-76. 1998
    ..These data suggest that OPGL is an osteoclast differentiation and activation factor. The effects of OPGL are blocked in vitro and in vivo by OPG, suggesting that OPGL and OPG are key extracellular regulators of osteoclast development...
  97. ncbi Structural basis for sorting mechanism of p62 in selective autophagy
    Yoshinobu Ichimura
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421
    J Biol Chem 283:22847-57. 2008
    ..These results demonstrate that the intracellular level of p62 is tightly regulated by autophagy through the direct interaction of LC3 with p62 and reveal that selective turnover of p62 via autophagy controls inclusion body formation...
  98. pmc Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance
    Yong Sheng Wang
    Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA
    Plant Cell 18:3635-46. 2006
    ..oryzae pv oryzae. Furthermore, reduced levels of Xb3 lead to decreased levels of the XA21 protein. These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance...
  99. ncbi Dynamic binding of histone H1 to chromatin in living cells
    T Misteli
    National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 408:877-81. 2000
    ..These results support a model in which linker histones bind dynamically to chromatin in a stop-and-go mode...
  100. ncbi The complete family of genes encoding G proteins of Caenorhabditis elegans
    G Jansen
    Division of Molecular Biology, The Netherlands Cancer Institute, Centre for Biomedical Genetics, Amsterdam
    Nat Genet 21:414-9. 1999
    ....
  101. pmc Deconstructing the cadherin-catenin-actin complex
    Soichiro Yamada
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 123:889-901. 2005
    ..These results suggest that the linkage between the cadherin-catenin complex and actin filaments is more dynamic than previously appreciated...

Research Grants62

  1. ANTIBODY FUSION PROTEINS FOR THE THERAPY OF CANCER
    Joseph Rosenblatt; Fiscal Year: 2002
    ..He will characterize the properties of the recombinant fusion proteins in vivo and their effectiveness in causing tumor rejection...
  2. Novel Recombinant Anticoagulant Proteins
    TZE CHEIN WUN; Fiscal Year: 2005
    ..3. Develop a bioprocess to produce the recombinant fusion proteins for in vitro and in vivo studies...
  3. Rapid Detection of Antibodies to Adenovirus 36
    Cynthia Chappell; Fiscal Year: 2013
    ..We have cloned and expressed four recombinant fusion proteins containing AdV36 HVRs and tested each using SNA-identified positive or negative sera...
  4. Targeted lanthanide contrast agents for in cellulo single molecule imaging
    Lawrence Miller; Fiscal Year: 2011
    ..characterize a general purpose method for the extracellular delivery of lanthanide probes to specific, recombinant fusion proteins. Methodologically, we will adapt epi-fluorescence microscopy for pulsed, near-UV excitation and time-..
  5. REGULATION OF THE NA+/K+ 2C1 COTRANSPORTER PROTEIN
    David Mount; Fiscal Year: 1999
    ..Antibodies will be generated to unique cytoplasmic fragments of rBSC, as expressed in recombinant fusion proteins. These antibodies will be used to characterize the rBSC protein and demonstrate that it is a ..
  6. Pharmacologic treatment of thromboembolism
    TZE CHEIN WUN; Fiscal Year: 2009
    ..EVAS Therapeutics, LLC has developed a series of recombinant fusion proteins, termed "ANV-KPIs"...
  7. IDENTIFICATION--ONCHOCERCA VOLVULUS PROTECTIVE EPITOPES
    THOMAS UNNASCH; Fiscal Year: 1993
    ..sequence analysis, and identification of the native parasite antigen sharing epitopes with each of the recombinant fusion proteins. The major epitopes encoded by each of the potentially protective clones will be experimentally ..
  8. Peptide antigens in CD45RBhigh CD4+T cell colitis model
    Lawrence Saubermann; Fiscal Year: 2003
    ..The primary goal is to identify expressed peptide(s) from recombinant fusion proteins created from cDNA libraries made from tissue and cecal bacteria extracts...
  9. RETROVIRAL GAG PROTEIN BINDING TO TYROSINE KINASES
    CORALIE CARRAWAY; Fiscal Year: 2001
    ..of p58gag, the p60gag MAIDS Gag, and HIV Gag with tyrosine kinases and other signaling proteins using recombinant fusion proteins, transfection studies, cell perturbation analyses and binding assays...
  10. Novel HIV Neutralizing Human MAbs from Transgenic Mice
    Abraham Pinter; Fiscal Year: 2006
    ..In preliminary studies, we have immunized Xenomice with a number of HIV envelope antigens, including recombinant fusion proteins expressing several of the variable loop domains of the viral surface protein, and a recombinant gpl20 ..
  11. ENHANCED B VIRUS DIAGNOSIS/CONTROL IN PRIMATE COLONIES
    Julia Hilliard; Fiscal Year: 2000
    ..4) utilize monoclonal and polyclonal monospecific sera prepared against synthetic peptides and recombinant fusion proteins for rapid assays to identify selected antibody and antigen specificities; (5) identify human monoclonal ..
  12. Low Cost Production of Recombinant Proteins
    SUSAN DAGHER; Fiscal Year: 2007
    ..Express six pharmaceutically-relevant, biogeneric candidate recombinant fusion proteins in E. coli and CHO; 2. Purify the proteins to at least 90% purity, as measured by LC-MS; and 3...
  13. Development of ADAM10 Prodomain as a Therapeutic Agent
    Peter Dempsey; Fiscal Year: 2009
    ..For example, recombinant fusion proteins consisting of the extracellular domain of the immunoregluatory proteins and the constant (Fc) domain of ..
  14. COTRANSLATIONAL PROCESSING AND PROTEIN TURNOVER
    RALPH BRADSHAW; Fiscal Year: 2001
    ..Cellular localization will also be determined with recombinant fusion proteins and immunological reagents...
  15. NEW TUMOR MODELS FOR THE DEVELOPMENT OF IMMUNOTHERAPY
    Ralph Reisfeld; Fiscal Year: 1992
    ..proteins, bispecific antibodies recognizing EGF-R or MTr and T cell markers such as CD3 and CD2, and recombinant fusion proteins consisting of chimeric Mabs and the cytotoxic domain of Pseudomonas exotoxin...
  16. Development of Novel Gold Binding Fusion Proteins
    MEHER IRANI; Fiscal Year: 2003
    ..optimize gold binding of the fusion proteins; d) to construct and characterize biosensors with these recombinant fusion proteins. The long-term aim of this project is to expand the expression capabilities to a host of other ..
  17. GLOMERULAR CAPILLARY WALL--NORMAL AND PATHOLOGIC
    Yashpal Kanwar; Fiscal Year: 2003
    ..III. Molecular protein: protein and RNA: protein interactions will be investigated by using recombinant fusion proteins of various constructs of these three molecules. IV...
  18. CANDIDATE PROTEIN OF THE B AMYLOID PRECURSOR COMPLEX
    Jan Teller; Fiscal Year: 1999
    ..We propose to study the XBP protein by characterizing its ability to interact with functional fragments of X11 both in vitro and in vivo by using recombinant fusion proteins and specific antibodies.
  19. Enhancing HIV-1 vaccine immunogenicity using hsp70
    Donald Harn; Fiscal Year: 2004
    ..We will also attempt to enhance TD158 plasmid DNA vaccine efficacy by boosting with TD158/HspT0 recombinant fusion proteins, formulated plus/minus a Th1-driving adjuvant...
  20. Neuronal precursor regulation during corticogenesis
    DANIEL SANTILLANO; Fiscal Year: 2003
    ..Using adenoviral gene transfer methods and recombinant fusion proteins for R-prominin, our studies will examine the mechanisms that effect the proliferation potential of ..
  21. PROTEOMIC DIS. OF CHLAMYDIA INCLUSION-HOST INTERACTIONS
    Christine Wu; Fiscal Year: 2006
    ..Finally, Specific Aim 3 will focus on the selected chlamydial proteins from Specific Aim 2 and generate recombinant fusion proteins to identify interacting host proteins in vitro.
  22. IMMUNOLOGICAL MECHANISM OF INFLAMMATION IN UVEITIS & EAE
    Grazyna Adamus; Fiscal Year: 2001
    ..3) Examine the role of cytokines and chemokines in the recruitment of cell to the eye in primary and recurrent uveitis associated with EAE after immunization of Lewis rats with MBP. ..
  23. Recombinant T-cell Receptor Ligands for Treatment of Uveitis
    Grazyna Adamus; Fiscal Year: 2006
    ..To determine an effective treatment regimen for clinical rat AAU using RTLs. 2. To determine the abilities of RTLs to inhibit recurrent anterior and posterior uveitis in Lewis rats. [unreadable] [unreadable] [unreadable] [unreadable]..
  24. MECHANISM OF RETINAL DAMAGE IN AUTOIMMUNE RETINOPATHY
    Grazyna Adamus; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  25. CUTANEOUS ONCOLOGY
    Madeleine Duvic; Fiscal Year: 2004
    ..Young physicians and students who receive advanced training in the proper detection, prevention, and treatment of skin cancers will be a resource and may improve outcomes for patients of the future. ..
  26. AIDS Neurotherapeutics and BBB Drug Efflux
    William Pardridge; Fiscal Year: 2007
    ..This work provides the basis for future drug discovery of AET blockers, which can be used as co-drugs to increase CNS penetration of HAART drugs. ..
  27. STRUCTURE AND FUNCTION OF THE LH AND FSH RECEPTORS
    Deborah Segaloff; Fiscal Year: 2002
    ..Long term goals of research in this area include the ability to better understand, diagnose, and correct reproductive disorders. ..
  28. A novel Dlg isoform and identification of its interacting proteins
    Kyung Ok Cho; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. Non-Viral Gene Targeting to the Brain
    William Pardridge; Fiscal Year: 2008
    ..abstract_text> ..
  30. BLys inhibition using TACI-Fc in patients with B-bell non-Hodgkins' Lymphoma
    Stephen Ansell; Fiscal Year: 2008
    ..abstract_text> ..
  31. OSTEOCLASTIC PHOSPHOTYROSYL PHOSPHATASE AND RESORPTION
    KIN HING LAU; Fiscal Year: 2002
    ....
  32. Gonadotropin Receptor Dimerization/Oligomerization
    Deborah Segaloff; Fiscal Year: 2008
    ..abstract_text> ..
  33. Mucosal M Cell Endocytosis: Specificity and Mechanisms
    David Lo; Fiscal Year: 2008
    ..Such insights will not only help understand the biology of mucosal immunity, but also provide potential strategies for developing effective mucosal vaccines. [unreadable] [unreadable] [unreadable] [unreadable]..
  34. Conference on Photodynamic Therapy
    David Kessel; Fiscal Year: 2002
    ..The most recent PDT meeting, sponsored by the International Photodynamic Assn, has more than 600 participants. ..
  35. 10th-12th Conferences: Drug Abuse/Immunomodulation/AIDS
    Thomas Rogers; Fiscal Year: 2005
    ..The combination of the web site, and a brochure mailing should guarantee wide dissemination of information regarding the meeting, and guarantee publicity for the published results. ..
  36. Novel Assessment of Early Changes in Diabetic Retinopathy
    Quan Dong Nguyen; Fiscal Year: 2010
    ..Such methods will be critically important to assessment of preventive interventions and monitoring diabetic eye disease in the future. ..
  37. The vital role of BAFF in the development of SLE
    William Stohl; Fiscal Year: 2010
    ..abstract_text> ..
  38. REGULATION OF AUTOIMMUNITY WITH T CELL RECEPTOR PEPTIDES
    Arthur A Vandenbark; Fiscal Year: 2010
    ..abstract_text> ..
  39. Gene drive potential of transposable elements in Anopheles gambiae.
    DAVID A O apos BROCHTA; Fiscal Year: 2010
    ..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
  40. Biochemical Modulation of Nociceptive Circuits
    MARK JOHN ZYLKA; Fiscal Year: 2010
    ..Finally, we will test the hypothesis that this phosphatase modulates neuropathic pain behaviors in wild-type and phosphatase knockout mice. ..
  41. ROLE OF FRIZZLED RECEPTORS AND EMBRYOGENESIS
    Stephen Ekker; Fiscal Year: 2001
    ..Using these tools, we will identify the signaling cascades mediated by this family of receptor- like molecules in an attempt to understand in a broader context the role this new gene family plays in development. ..
  42. Single Molecule Studies of DNA Helicases
    PIERO BIANCO; Fiscal Year: 2007
    ..abstract_text> ..
  43. GENISTEIN AS MODULATOR OF ANTIOXIDANT PROTEIN EXPRESSION
    Shiu Ming Kuo; Fiscal Year: 2002
    ..These studies may also lead to the identification of a previous unknown pathway for Gn to regulate mammalian genes. ..
  44. BLOOD BRAIN BARRIER LARGE NEUTRAL AMINO ACID TRANSPORTER
    William Pardridge; Fiscal Year: 2003
    ..These studies will provide new molecular biological information on a crucial transporter at the blood-brain barrier that regulates the supply in the brain of essential amino acids. ..
  45. Metabolism of Carcinogens and Drugs by Human P450s
    F Guengerich; Fiscal Year: 2005
    ..Successful completion of these collective studies should provide better understanding of the ways in which human P450s catalyze oxidation of carcinogens and drugs and the significance of variations in these enzymes. ..
  46. TWO X-LINKED GENES THAT REGULATE MINERAL HOMEOSTASIS
    Michael Whyte; Fiscal Year: 2002
    ....
  47. Transgenic Rod Development and Function
    Baerbel Rohrer; Fiscal Year: 2005
    ..However, more importantly, it will provide avenues for studying gene defects at the single cell level not only in the retina, but in other systems as well. ..
  48. Novel regulatory role of cryopyrin in inflammation
    Harold Hoffman; Fiscal Year: 2005
    ..abstract_text> ..
  49. In vivo panning for schistosome protective epitopes
    Charles Shoemaker; Fiscal Year: 2005
    ....
  50. Promotion of PDT Induced phototoxicity by bile acids
    David Kessel; Fiscal Year: 2003
    ..If the results of preliminary studies are borne out by further investigation, UDCA could offer a safe and effective means for promoting clinical PDT efficacy. ..
  51. FAE specific receptors for targeted vaccine delivery
    David Lo; Fiscal Year: 2004
    ..The development of successful targeting ligands would comprise a novel platform for the development of synthetic vaccines, especially for the induction of mucosal immunity against agents such as influenza. ..
  52. Supplemental Oxygen for Hypoxia-Related Retinal Deseases
    Quan Nguyen; Fiscal Year: 2005
    ....
  53. Development of a T cell specific multiple sclerosis drug
    Arthur Vandenbark; Fiscal Year: 2005
    ..The proposed studies will address key milestones to establish clinical safety of VG1000 needed for subsequent proof-of-principle trials to evaluate inhibition of MOG-specific T cell responses and clinical efficacy. ..
  54. Analysis of Toxin A synthesis in P. aeruginosa
    Abdul Hamood; Fiscal Year: 2006
    ....
  55. REGULATION OF BBB GLUT1 GLUCOSE TRANSPORTER
    William Pardridge; Fiscal Year: 2002
    ..These studies will provide insight into molecular mechanisms of regulation of a step crucial to the maintenance of cerebral intermediary metabolism, i.e., the continuous transport of glucose across the blood-brain barrier in vivo. ..
  56. Bioreactor for Engineered Bioartificial Tissues (BATS)
    Albert Banes; Fiscal Year: 2005
    ..Expansion to a larger bioreactor chamber will allow biomedical researchers to develop better strategies for growth and testing of scaled up tissues for use in vivo in numerous connective tissue applications. ..
  57. DerG (a new immunomodulator) Treatment of Viral Encepha*
    KENNETH ROSENTHAL; Fiscal Year: 2005
    ....
  58. Structural Studies of NAGS
    Dashuang Shi; Fiscal Year: 2006
    ..abstract_text> ..
  59. Elucidate C. elegans Oxygen Sensing with Microfluidics
    Hang Lu; Fiscal Year: 2006
    ..The general techniques developed in this study should impact other C. elegans researches of sensing and behaviors such as odor discrimination and thermo-sensation. ..
  60. Engineering DNA nicking endonucleases
    Shuang yong Xu; Fiscal Year: 2006
    ..Under Phase II funding, this isothermal amplification method (NEMDA) will be optimized and the sensitivity will be improved. Finally, we will investigate the potential of NEMDA for detections of pathogens and its use in bio-prospecting. ..
  61. Enhancing Immunotherapy through Toll Like Receptors
    Cynthia Leifer; Fiscal Year: 2007
    ..This career transition grant will allow me to develop a research program focused on new ways to exploit TLR-PAMP biology for the treatment of human cancer. ..