protein subunits

Summary

Summary: Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.

Top Publications

  1. pmc Glutamate receptor ion channels: structure, regulation, and function
    Stephen F Traynelis
    Department of Pharmacology, Emory University School of Medicine, Rollins Research Center, 1510 Clifton Road, Atlanta, GA 30322 3090, USA
    Pharmacol Rev 62:405-96. 2010
  2. pmc Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2
    Scott B Hansen
    Laboratory of Molecular Neurobiology and Biophysics, The Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, New York 10065, USA
    Nature 477:495-8. 2011
  3. ncbi Crystal structure of the sodium-potassium pump at 2.4 A resolution
    Takehiro Shinoda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 459:446-50. 2009
  4. ncbi Crystal structure of the sodium-potassium pump
    J Preben Morth
    Centre for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Nature 450:1043-9. 2007
  5. ncbi Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7
    Rob D Chapman
    Institute of Clinical Molecular Biology and Tumour Genetics, GSF Research Center of Environment and Health, Munich Center for Integrated Protein Science CIPSM, Marchioninistrasse 25, 81377 Munich, Germany
    Science 318:1780-2. 2007
  6. ncbi Refined structure of the nicotinic acetylcholine receptor at 4A resolution
    Nigel Unwin
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 346:967-89. 2005
  7. pmc X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor
    Alexander I Sobolevsky
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nature 462:745-56. 2009
  8. ncbi Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:897-903. 2005
  9. ncbi NMDA receptor subunits: function and pharmacology
    Pierre Paoletti
    Laboratoire de Neurobiologie, CNRS UMR 8544 Ecole Normale Supérieure 46 rue d Ulm, 75005 Paris, France
    Curr Opin Pharmacol 7:39-47. 2007
  10. ncbi NMDA receptor subunits: diversity, development and disease
    S Cull-Candy
    Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, UK
    Curr Opin Neurobiol 11:327-35. 2001

Detail Information

Publications318 found, 100 shown here

  1. pmc Glutamate receptor ion channels: structure, regulation, and function
    Stephen F Traynelis
    Department of Pharmacology, Emory University School of Medicine, Rollins Research Center, 1510 Clifton Road, Atlanta, GA 30322 3090, USA
    Pharmacol Rev 62:405-96. 2010
    ....
  2. pmc Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2
    Scott B Hansen
    Laboratory of Molecular Neurobiology and Biophysics, The Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, New York 10065, USA
    Nature 477:495-8. 2011
    ....
  3. ncbi Crystal structure of the sodium-potassium pump at 2.4 A resolution
    Takehiro Shinoda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 459:446-50. 2009
    ....
  4. ncbi Crystal structure of the sodium-potassium pump
    J Preben Morth
    Centre for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Nature 450:1043-9. 2007
    ..The carboxy terminus of the alpha-subunit is contained within a pocket between transmembrane helices and seems to be a novel regulatory element controlling sodium affinity, possibly influenced by the membrane potential...
  5. ncbi Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7
    Rob D Chapman
    Institute of Clinical Molecular Biology and Tumour Genetics, GSF Research Center of Environment and Health, Munich Center for Integrated Protein Science CIPSM, Marchioninistrasse 25, 81377 Munich, Germany
    Science 318:1780-2. 2007
    ..Our results indicate that restriction of serine-7 epitopes to the Linker-proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression...
  6. ncbi Refined structure of the nicotinic acetylcholine receptor at 4A resolution
    Nigel Unwin
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 346:967-89. 2005
    ..Access to the pore on the intracellular side is further influenced by narrow lateral windows, which would be expected to screen out electrostatically ions of the wrong charge and size...
  7. pmc X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor
    Alexander I Sobolevsky
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nature 462:745-56. 2009
    ..We exploit the GluA2 structure to develop mechanisms of ion channel activation, desensitization and inhibition by non-competitive antagonists and pore blockers...
  8. ncbi Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:897-903. 2005
    ..Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit...
  9. ncbi NMDA receptor subunits: function and pharmacology
    Pierre Paoletti
    Laboratoire de Neurobiologie, CNRS UMR 8544 Ecole Normale Supérieure 46 rue d Ulm, 75005 Paris, France
    Curr Opin Pharmacol 7:39-47. 2007
    ....
  10. ncbi NMDA receptor subunits: diversity, development and disease
    S Cull-Candy
    Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, UK
    Curr Opin Neurobiol 11:327-35. 2001
    ..Recent studies are beginning to address the functional significance of NMDAR diversity under normal and pathological conditions...
  11. ncbi Biochemistry of Na,K-ATPase
    Jack H Kaplan
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97201, USA
    Annu Rev Biochem 71:511-35. 2002
    ..In this review, the current status of knowledge of Na,K-ATPase is discussed, and areas where there is still considerable uncertainty are highlighted...
  12. pmc International Union of Pharmacology. LXX. Subtypes of gamma-aminobutyric acid(A) receptors: classification on the basis of subunit composition, pharmacology, and function. Update
    Richard W Olsen
    Department of Molecular and Medical Pharmacology, Geffen School of Medicine at UCLA, Room CHS 23 120, 650 Young Drive South, Los Angeles, CA 90095 1735, USA
    Pharmacol Rev 60:243-60. 2008
    ..The list is divided into three categories of native receptor subtypes: "identified," "existence with high probability," and "tentative."..
  13. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ....
  14. pmc Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex
    Henri Marc Bourbon
    Centre de Biologie du Developpement, UMR5547 CNRS Toulouse III, IFR109, Universite Paul Sabatier, 31062 Toulouse, France
    Nucleic Acids Res 36:3993-4008. 2008
    ..The implications of this comprehensive work for MED structure-function relationships are discussed...
  15. ncbi Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle
    Huaqing Cai
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA
    Dev Cell 12:671-82. 2007
    ..However, recent findings have revealed a role for the coat in directing a vesicle to its correct intracellular destination. In this review we will discuss the literature that links coat proteins to vesicle targeting events...
  16. pmc Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry
    Zhuo Angel Chen
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, The University of Edinburgh, Edinburgh, UK
    EMBO J 29:717-26. 2010
    ..This work establishes cross-linking/MS as an integrated structure analysis tool for large multi-protein complexes...
  17. ncbi RNA polymerase IV directs silencing of endogenous DNA
    A J Herr
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Science 308:118-20. 2005
    ..The existence of this distinct silencing polymerase may explain the paradoxical involvement of an RNA silencing pathway in maintenance of transcriptional silencing...
  18. ncbi Molecular architecture of SMC proteins and the yeast cohesin complex
    Christian H Haering
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 9:773-88. 2002
    ..The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication...
  19. ncbi Structural basis for modulation and agonist specificity of HCN pacemaker channels
    William N Zagotta
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Box 357290, University of Washington School of Medicine, Seattle, Washington 98195 7290, USA
    Nature 425:200-5. 2003
    ..On the basis of amino acid sequence similarity to HCN channels, the cyclic nucleotide-gated, and eag- and KAT1-related families of channels are probably related to HCN channels in structure and mechanism...
  20. ncbi Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme
    Uhn Soo Cho
    Department of Biological Structure, University of Washington, Seattle, Washington 98195, USA
    Nature 445:53-7. 2007
    ..Together, our structural results establish a crucial foundation for understanding PP2A assembly, substrate recruitment and regulation...
  21. ncbi Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Michael J Carrozza
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell 123:581-92. 2005
    ..This erases transcription elongation-associated acetylation to suppress intragenic transcription initiation...
  22. ncbi Architecture of succinate dehydrogenase and reactive oxygen species generation
    Victoria Yankovskaya
    Molecular Biology Division, VA Medical Center, San Francisco, CA 94121, USA
    Science 299:700-4. 2003
    ..Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme...
  23. ncbi The Mcm2-7 complex has in vitro helicase activity
    Matthew L Bochman
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Cell 31:287-93. 2008
    ..Our results show that purified Mcm2-7 acts as a helicase, provides functional evidence of a Mcm2/5 gate, and lays the foundation for future mechanistic studies of this critical factor...
  24. pmc Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by alpha5 subunit-containing gamma-aminobutyric acid type A receptors
    Valerie B Caraiscos
    Institute of Medical Science, Department of Anesthesia, University of Toronto, Toronto, ON, Canada M5S 1A8
    Proc Natl Acad Sci U S A 101:3662-7. 2004
    ....
  25. pmc Proteasome subunit Rpn13 is a novel ubiquitin receptor
    Koraljka Husnjak
    Institute of Biochemistry II and Cluster of Excellence Macromolecular Complexes, Goethe University, Theodor Stern Kai 7, D 60590 Frankfurt Main, Germany
    Nature 453:481-8. 2008
    ..Because Rpn13 is also the proteasomal receptor for Uch37, a deubiquitinating enzyme, our findings suggest a coupling of chain recognition and disassembly at the proteasome...
  26. ncbi Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity
    Benjamin D Rowland
    University of Oxford, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
    Mol Cell 33:763-74. 2009
    ..According to this model, Eco1 promotes sister DNA entrapment partly by relieving an antiestablishment activity associated with Scc3, Pds5, and Rad61...
  27. pmc Assembly reflects evolution of protein complexes
    Emmanuel D Levy
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 453:1262-5. 2008
    ..Our model of self-assembly allows reliable prediction of evolution and assembly of a complex solely from its crystal structure...
  28. ncbi The antidiabetic drug metformin activates the AMP-activated protein kinase cascade via an adenine nucleotide-independent mechanism
    Simon A Hawley
    Division of Molecular Physiology, School of Life Sciences and Wellcome Trust Biocentre, Dundee University, Dundee, Scotland, UK
    Diabetes 51:2420-5. 2002
    ..Our results also show that AMPK can be activated by mechanisms other than changes in the cellular AMP-to-ATP ratio...
  29. pmc Subunit arrangement and phenylethanolamine binding in GluN1/GluN2B NMDA receptors
    Erkan Karakas
    Cold Spring Harbor Laboratory, WM Keck Structural Biology Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 475:249-53. 2011
    ..These findings pave the way for improving the design of subtype-specific compounds with therapeutic value for neurological disorders and diseases...
  30. ncbi Architecture of the photosynthetic oxygen-evolving center
    Kristina N Ferreira
    Department of Biological Sciences, Imperial College London, London, SW7 2AZ, UK
    Science 303:1831-8. 2004
    ..The details of the surrounding coordination sphere of the metal cluster and the implications for a possible oxygen-evolving mechanism are discussed...
  31. ncbi NDUFA4 is a subunit of complex IV of the mammalian electron transport chain
    Eduardo Balsa
    Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, 28029, Spain
    Cell Metab 16:378-86. 2012
    ..The change in the attribution of the NDUFA4 protein requires renaming of the gene and reconsideration of the structure of CIV. Furthermore, NDUFA4 should be considered a candidate gene for CIV rather than CI deficiencies in humans...
  32. pmc Mediator influences telomeric silencing and cellular life span
    Xuefeng Zhu
    Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Medicinaregatan 9A, SE 40530 Goteborg, Sweden
    Mol Cell Biol 31:2413-21. 2011
    ..Increases in H4K16 acetylation have been observed at telomeres in aging cells. In agreement with this observation, we found that the loss of MED5 leads to shortening of the Saccharomyces cerevisiae (budding yeast) replicative life span...
  33. ncbi Allostery wiring diagrams in the transitions that drive the GroEL reaction cycle
    RIINA TEHVER
    Biophysics Program, Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA
    J Mol Biol 387:390-406. 2009
    ..We find that several substrate protein binding residues as well as sites related to ATPase activity belong to a single functional network in GroEL. For GroES, the mobile loop residues and GroES/GroES interface residues are linked...
  34. pmc Reconstitution reveals the functional core of mammalian eIF3
    Mamiko Masutani
    RIKEN Genomic Sciences Center, Tsurumi ku, Yokohama, Japan
    EMBO J 26:3373-83. 2007
    ..Extensive deletion analyses suggest that three evolutionarily conserved subunits (eIF3a, eIF3b, and eIF3c) and three non-conserved subunits (eIF3e, eIF3f, and eIF3h) comprise the functional core of mammalian eIF3...
  35. ncbi Subunit arrangement and function in NMDA receptors
    Hiroyasu Furukawa
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, New York, New York 10032, USA
    Nature 438:185-92. 2005
    ....
  36. ncbi mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events
    Marina K Holz
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 123:569-80. 2005
    ..Thus, the eIF3 preinitiation complex acts as a scaffold to coordinate a dynamic sequence of events in response to stimuli that promote efficient protein synthesis...
  37. ncbi A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II
    Henri Marc Bourbon
    Mol Cell 14:553-7. 2004
  38. ncbi Multiple regulatory sites in large-conductance calcium-activated potassium channels
    Xiao Ming Xia
    Department of Anesthesiology, Washington University School of Medicine, Box 8054, St Louis, Missouri 63110, USA
    Nature 418:880-4. 2002
    ..This allows BK channels to serve a variety of physiological roles contingent on the Ca(2+) concentration to which the channels are exposed...
  39. ncbi Germ-line mutations in nonsyndromic pheochromocytoma
    Hartmut P H Neumann
    Department of Nephrology and Hypertension, Albert Ludwigs University, Freiburg, Germany
    N Engl J Med 346:1459-66. 2002
    ....
  40. ncbi 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors
    Paola Grandi
    Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Mol Cell 10:105-15. 2002
    ..We conclude that the 40S synthesis machinery predominately associates with the 35S pre-rRNA factors, whereas factors required for 60S subunit synthesis largely bind later, showing an unexpected dichotomy in binding...
  41. ncbi Functional architecture of RNA polymerase I
    Claus D Kuhn
    Gene Center Munich and Center for Integrated Protein Science CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, 81377 Munich, Germany
    Cell 131:1260-72. 2007
    ..In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming...
  42. ncbi Molecular basis of NMDA receptor functional diversity
    Pierre Paoletti
    Institut de Biologie de l Ecole Normale Supérieure, Ecole Normale Superieure, Inserm U1024, 75005 Paris, France
    Eur J Neurosci 33:1351-65. 2011
    ..Indeed, the identity of this region, which is distal to the membrane and precedes the agonist-binding domains, determines key biophysical and pharmacological attributes of the various NMDAR subtypes...
  43. pmc Sorting of the Alzheimer's disease amyloid precursor protein mediated by the AP-4 complex
    Patricia V Burgos
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 18:425-36. 2010
    ....
  44. ncbi Structural divergence of the rotary ATPases
    Stephen P Muench
    Institute of Membrane and Systems Biology, Faculty of Biological Sciences, The University of Leeds, Leeds, West Yorks, LS2 9JT, UK
    Q Rev Biophys 44:311-56. 2011
    ..In particular, it discusses how newly revealed variation in the complexity of the inter-domain connections may impact on the mechanics and regulation of these molecular machines...
  45. ncbi PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation
    Zhanyun Tang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA
    Dev Cell 10:575-85. 2006
    ..Our findings suggest that Bub1 targets PP2A to centromeres, which in turn maintains Sgo1 at centromeres by counteracting Plk1-mediated chromosome removal of Sgo1...
  46. ncbi A cryptic protease couples deubiquitination and degradation by the proteasome
    Tingting Yao
    Department of Biochemistry, University of Iowa, 51 Newton Road, Iowa City, Iowa 52242, USA
    Nature 419:403-7. 2002
    ..The inability to remove ubiquitin can be rate-limiting for degradation in vitro and is lethal to yeast. Unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases, POH1 appears to be a Zn(2+)-dependent protease...
  47. pmc Identification of PP2A complexes and pathways involved in cell transformation
    Anna A Sablina
    Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Cancer Res 70:10474-84. 2010
    ..These observations delineate the PP2A family members and pathways perturbed by SV40ST during human cell transformation...
  48. ncbi A gated channel into the proteasome core particle
    M Groll
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Nat Struct Biol 7:1062-7. 2000
    ..Inhibition by the alpha-subunit tails is relieved upon binding of the regulatory particle to the CP to form the proteasome holoenzyme...
  49. ncbi Crystal structure of mitochondrial respiratory membrane protein complex II
    Fei Sun
    Tsinghua IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, China
    Cell 121:1043-57. 2005
    ..The Complex II structure provides a bona fide model for study of the mitochondrial respiratory system and human mitochondrial diseases related to mutations in this complex...
  50. ncbi NMDA receptor subunits have differential roles in mediating excitotoxic neuronal death both in vitro and in vivo
    Yitao Liu
    Brain Research Centre, Vancouver Coastal Health Research Institute and University of British Columbia, Vancouver, British Columbia, Canada V6T 2B5
    J Neurosci 27:2846-57. 2007
    ....
  51. pmc Protein kinase A activates protein phosphatase 2A by phosphorylation of the B56delta subunit
    Jung Hyuck Ahn
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:2979-84. 2007
    ..Moreover, stimulation of protein phosphatase activity by this mechanism may represent an important signaling pathway regulated by cAMP in neurons and other types of cell...
  52. ncbi DNA polymerase gamma, the mitochondrial replicase
    Laurie S Kaguni
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 1319, USA
    Annu Rev Biochem 73:293-320. 2004
    ..This review describes our current understanding of pol gamma biochemistry and biology, and it introduces other key proteins that function at the mitochondrial DNA replication fork...
  53. pmc Structure of the gating ring from the human large-conductance Ca(2+)-gated K(+) channel
    Yunkun Wu
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Nature 466:393-7. 2010
    ....
  54. ncbi Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae
    M L Mayer
    Graduate Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Cell 7:959-70. 2001
    ....
  55. pmc Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution
    Peng Yuan
    Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA
    Science 329:182-6. 2010
    ..The structure suggests that the Ca2+ gating ring, in addition to regulating the pore directly, may also modulate the voltage sensor...
  56. ncbi Structural basis of RNA polymerase II backtracking, arrest and reactivation
    Alan C M Cheung
    Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, 81377 Munich, Germany
    Nature 471:249-53. 2011
    ..These results establish the structural basis of Pol II backtracking, arrest and reactivation, and provide a framework for analysing gene regulation during transcription elongation...
  57. pmc Subunit interactions at the V1-Vo interface in yeast vacuolar ATPase
    Rebecca A Oot
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 287:13396-406. 2012
    ....
  58. pmc A universal protein-protein interaction motif in the eubacterial DNA replication and repair systems
    B P Dalrymple
    Commonwealth Scientific and Industrial Research Organisation Livestock Industries, 120 Meiers Road, Indooroopilly QLD 4068, Australia
    Proc Natl Acad Sci U S A 98:11627-32. 2001
    ..Comparison of the eubacterial, eukaryotic, and archaeal sliding clamp binding motifs suggests that the basic interactions have been conserved across the evolutionary landscape...
  59. ncbi Ancillary subunits associated with voltage-dependent K+ channels
    Olaf Pongs
    Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Universitatsklinikum Hamburg Eppendorf, Universitat Hamburg, Hamburg, Germany
    Physiol Rev 90:755-96. 2010
    ..We critically review present knowledge on the physiological role of ancillary Kv channel subunits and their effects on Kv channel properties...
  60. pmc ATP binds to proteasomal ATPases in pairs with distinct functional effects, implying an ordered reaction cycle
    David M Smith
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 144:526-38. 2011
    ..These results can explain how these hexameric ATPases interact with and "wobble" on top of the heptameric 20S proteasome...
  61. pmc Role of the RNA polymerase trigger loop in catalysis and pausing
    Jinwei Zhang
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA
    Nat Struct Mol Biol 17:99-104. 2010
    ..The similar effects of TL/TH substitutions on pausing and nucleotide addition provide additional support for the view that TH formation is rate-limiting for escape from nonbacktracked pauses...
  62. pmc Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 A resolution
    Xiao Tao
    Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA
    Science 326:1668-74. 2009
    ..These same surface features also suggest a possible approach to the development of inhibitory agents specific to each member of the inward-rectifier K+ channel family...
  63. pmc Ribosomal protein L7Ae is a subunit of archaeal RNase P
    I Ming Cho
    Department of Biochemistry and Molecular Genetics, Center for RNA Biology, Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 107:14573-8. 2010
    ..After establishing in vitro reconstitution of the single RNA with four previously known protein subunits (POP5, RPP21, RPP29, and RPP30), we show that addition of L7Ae to this RNase P complex increases the optimal ..
  64. pmc Pharmacological characterization of a novel cell line expressing human alpha(4)beta(3)delta GABA(A) receptors
    N Brown
    Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Terlings Park, Eastwick Road, Harlow, Essex CM20 2QR, UK
    Br J Pharmacol 136:965-74. 2002
    ..With its restricted distribution within the brain and unique pharmacology this receptor may play an important role in the action of neurosteroids and anaesthetics. British Journal of Pharmacology (2002) 136, 965-974..
  65. ncbi Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, U K
    Science 311:1430-6. 2006
    ..The novel subunit Nqo15 has a similar fold to the mitochondrial iron chaperone frataxin, and it may be involved in iron-sulfur cluster regeneration in the complex...
  66. pmc Diversity of protein-protein interactions
    Irene M A Nooren
    European Bioinformatics Institute EMBL EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
    EMBO J 22:3486-92. 2003
    ..Structural characteristics of different types of PPIs are discussed and related to their physiological function, specificity and evolution...
  67. ncbi Common architecture of the flagellar type III protein export apparatus and F- and V-type ATPases
    Tatsuya Ibuki
    Osaka University, Osaka, Japan
    Nat Struct Mol Biol 18:277-82. 2011
    ..These results suggest that the type III protein export system and F- and V-type ATPases share a similar mechanism and an evolutionary relationship...
  68. pmc The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons
    Wen Ling Chiu
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:4415-34. 2010
    ....
  69. ncbi Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutières syndrome and mimic congenital viral brain infection
    Yanick J Crow
    Leeds Institute of Molecular Medicine, University of Leeds, St James s University Hospital, Leeds, LS9 7TF, UK
    Nat Genet 38:910-6. 2006
    ..Our findings demonstrate a role for ribonuclease H in human neurological disease and suggest an unanticipated relationship between ribonuclease H2 and the antiviral immune response that warrants further investigation...
  70. ncbi How subunits cooperate in cAMP-induced activation of homotetrameric HCN2 channels
    Jana Kusch
    Institut fur Physiologie II, Universitatsklinikum Jena, Friedrich Schiller Universitat Jena, Jena, Germany
    Nat Chem Biol 8:162-9. 2012
    ....
  71. ncbi Crystal structure of Arp2/3 complex
    R C Robinson
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 294:1679-84. 2001
    ..ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament...
  72. ncbi The mammalian Mediator complex and its role in transcriptional regulation
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Trends Biochem Sci 30:250-5. 2005
    ..Recent studies have defined the subunit composition and associated activities of mammalian Mediator, and revealed a striking evolutionary conservation of Mediator structure and function from yeast to man...
  73. pmc Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase
    Y Chi
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Genes Dev 15:1078-92. 2001
    ..Simultaneous down-regulation of both transcriptional regulatory proteins and RNA polymerase may enhance the potency and specificity of transcriptional inhibition by Srb10...
  74. ncbi Evidence for an unfolding/threading mechanism for protein disaggregation by Saccharomyces cerevisiae Hsp104
    Ronnie Lum
    Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 279:29139-46. 2004
    ....
  75. ncbi Protein disulfide isomerase acts as a redox-dependent chaperone to unfold cholera toxin
    B Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 104:937-48. 2001
    ....
  76. pmc Mutations in SCN9A, encoding a sodium channel alpha subunit, in patients with primary erythermalgia
    Y Yang
    Department of Dermatology, Peking University First Hospital, Beijing, China
    J Med Genet 41:171-4. 2004
    ..SCN9A, encoding a voltage-gated sodium channel alpha subunit predominantly expressed in sensory and sympathetic neurones, may play an important role in nociception and vasomotor regulation...
  77. pmc Analysis of the dynein-dynactin interaction in vitro and in vivo
    Stephen J King
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Mol Biol Cell 14:5089-97. 2003
    ..The dynein IC fragments have different effects on endomembrane localization, suggesting that different endomembranes may bind dynein via distinct mechanisms...
  78. ncbi Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors
    Jochen Schwenk
    Institute of Physiology II, University of Freiburg, Engesserstrasse 4, 79108 Freiburg, Germany
    Science 323:1313-9. 2009
    ..These results demonstrate that cornichons are intrinsic auxiliary subunits of native AMPARs and provide previously unknown molecular determinants for glutamatergic neurotransmission in the central nervous system...
  79. pmc Human MUC1 carcinoma-associated protein confers resistance to genotoxic anticancer agents
    Jian Ren
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 5:163-75. 2004
    ..These findings indicate that MUC1 attenuates the apoptotic response to DNA damage and that this oncoprotein confers resistance to genotoxic anticancer agents...
  80. pmc Location of modulatory beta subunits in BK potassium channels
    Guoxia Liu
    Division of Cardiology, Department of Medicine, LaGuardia Community College, Long Island City, NY 11101, USA
    J Gen Physiol 135:449-59. 2010
    ..Thus, each beta lies between and can interact with the voltage-sensing domains of two adjacent alpha subunits...
  81. pmc The MIS12 complex is a protein interaction hub for outer kinetochore assembly
    Arsen Petrovic
    Department of Experimental Oncology, European Institute of Oncology IEO, Milan, Italy
    J Cell Biol 190:835-52. 2010
    ..Our analysis has important implications for understanding kinetochore organization in different organisms...
  82. pmc LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1
    Jose M Lizcano
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 23:833-43. 2004
    ..Between them, these kinases may mediate the physiological effects of LKB1, including its tumour suppressor function...
  83. pmc The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila
    P Robin Hiesinger
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 121:607-20. 2005
    ..Our data suggest that Vha100-1 functions downstream of SNAREs in synaptic vesicle fusion...
  84. ncbi Signalling by PI3K isoforms: insights from gene-targeted mice
    Bart Vanhaesebroeck
    Ludwig Institute for Cancer Research, 91 Riding House Street, London W1W 7BS, UK
    Trends Biochem Sci 30:194-204. 2005
    ..Targeting strategies that take into account the complex interplay between members of the PI3K family will be crucial to gain a full understanding of the physiological roles of the isoforms of PI3K...
  85. pmc Prion protein attenuates excitotoxicity by inhibiting NMDA receptors
    Houman Khosravani
    Department of Physiology and Biophysics, Hotchkiss Brain Institute, University of Calgary, Calgary T2N4N1, Canada
    J Cell Biol 181:551-65. 2008
    ..Thus, native PrP(C) mediates an important neuroprotective role by virtue of its ability to inhibit NR2D subunits...
  86. ncbi Subunit composition, distribution and function of GABA(A) receptor subtypes
    W Sieghart
    Section of Biochemical Psychiatry, University Clinic for Psychiatry, and Department of Biochemistry and Molecular Biology, Brain Research Institute, University of Vienna, Vienna, Austria
    Curr Top Med Chem 2:795-816. 2002
    ..Such drugs might exhibit quite selective clinical effects...
  87. ncbi An extrasynaptic GABAA receptor mediates tonic inhibition in thalamic VB neurons
    Fan Jia
    CV Starr Laboratory for Molecular Neuropharmacology, Department of Anesthesiology, Weill Medical College, Cornell University, New York, NY 10021, USA
    J Neurophysiol 94:4491-501. 2005
    ..In view of the critical role of thalamic neurons in the generation of oscillatory activity associated with sleep, these receptors may represent a principal site of action for the novel hypnotic agent gaboxadol...
  88. ncbi Dynamic regulation of pol II transcription by the mammalian Mediator complex
    Sohail Malik
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, 1230 York Ave, New York, NY 10021, USA
    Trends Biochem Sci 30:256-63. 2005
    ..In addition, the compositional complexity of the Mediator allows for assimilation of other diverse signals such as those emanating from repressors and other coactivators...
  89. pmc An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation
    Silke Busch
    Institut fur Mikrobiologie und Genetik, Georg August Universitat, Grisebachstrasse 8, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 104:8089-94. 2007
    ..We propose that a correctly assembled CSN including a functional JAMM links protein turnover to fungal sexual development...
  90. pmc Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase
    Rita Gandhi
    Cold Spring Harbor Laboratory, One Bungtown Road, P O Box 100, Cold Spring Harbor, New York 11724, USA
    Curr Biol 16:2406-17. 2006
    ..This process is known as sister-chromatid resolution. Although two mitotic kinases have been implicated in this process, it remains unknown exactly how the cohesin-mediated linkage is destabilized at a mechanistic level...
  91. ncbi Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR epsilon 2 (NR2B) subunit of the N-methyl-D-aspartate receptor
    T Nakazawa
    Department of Oncology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Biol Chem 276:693-9. 2001
    ..These data suggest that Tyr-1472 phosphorylation of GluR epsilon 2 is important for synaptic plasticity...
  92. pmc New advances in NMDA receptor pharmacology
    Kevin K Ogden
    Department of Pharmacology, Emory University School of Medicine, 5025 Rollins Research Center, 1510 Clifton Road, Atlanta, GA 30322, USA
    Trends Pharmacol Sci 32:726-33. 2011
    ..Here, we review the properties of new emerging classes of subunit-selective NMDA receptor modulators, which we predict will mark the beginning of a productive period of progress for NMDA receptor pharmacology...
  93. pmc A scaffold of accessory subunits links the peripheral arm and the distal proton-pumping module of mitochondrial complex I
    Heike Angerer
    Medical School, Center for Membrane Proteomics, Goethe University, Frankfurt am Main, Germany
    Biochem J 437:279-88. 2011
    ....
  94. pmc Crystal structure of the extracellular segment of integrin alpha Vbeta3
    J P Xiong
    Renal Unit, Leukocyte Biology and Inflammation Program, Structural Biology Program, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
    Science 294:339-45. 2001
    ..MIDAS lies adjacent to a calcium-binding site with a potential regulatory function...
  95. pmc The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection
    Latifa ElAntak
    Structural Studies Division, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England, UK
    J Mol Biol 396:1097-116. 2010
    ..Taken together, we propose that eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RRM and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition...
  96. ncbi Structure of the membrane domain of respiratory complex I
    Rouslan G Efremov
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Nature 476:414-20. 2011
    ..The structure indicates that proton translocation in complex I, uniquely, involves coordinated conformational changes in six symmetrical structural elements...
  97. pmc Crystal structure of A3B3 complex of V-ATPase from Thermus thermophilus
    Megan J Maher
    Division of Molecular Biosciences, Imperial College London, South Kensington Campus, London, UK
    EMBO J 28:3771-9. 2009
    ..In the catalytic interface, our mutational analysis, on the basis of the A(3)B(3) structure, has highlighted the presence of a cluster composed of key hydrophobic residues, which are essential for ATP hydrolysis by V-ATPases...
  98. pmc The exozyme model: a continuum of functionally distinct complexes
    Daniel L Kiss
    Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4960, USA
    RNA 17:1-13. 2011
    ..The exozyme model is consistent with all published data and provides a new framework for understanding the general mechanisms and regulation of RNA processing and turnover...
  99. ncbi 5-HT3 receptors mediate serotonergic fast synaptic excitation of neocortical vasoactive intestinal peptide/cholecystokinin interneurons
    Isabelle Ferezou
    Laboratoire de Neurobiologie et Diversité Cellulaire, Centre National de la Recherche Scientifique, Unité Mixte de Rechérche 7637, Ecole Superieure de Physique et Chime Industrielles de la ville de Paris, 75005 Paris, France
    J Neurosci 22:7389-97. 2002
    ....
  100. ncbi RNA polymerase I contains a TFIIF-related DNA-binding subcomplex
    Sebastian R Geiger
    Gene Center and Department of Biochemistry, Center for Integrated Protein Science CIPSM, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Mol Cell 39:583-94. 2010
    ..Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity...
  101. pmc Position of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex
    Jesse Eichner
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    EMBO J 29:706-16. 2010
    ..Consistent with this mechanism, mutations far from the enzyme active site, which alter the binding of either structured TFIIF domains to Pol II, have similar defects in transcription start site usage...

Research Grants73

  1. Complex I: Role of L Subunit in Proton Translocation
    Steven B Vik; Fiscal Year: 2011
    ..Complex I subunits are targeted by proteases that trigger cell death processes. Mutations in the protein subunits of Complex I are risk factors for a wide range of diseases and disorders, such as multiple sclerosis, Type 2 ..
  2. Diverse Roles for RIC8 During G Protein Signaling in Fungi
    Katherine A Borkovich; Fiscal Year: 2012
    ..The stability of G protein subunits in ?ric8 and G?? mutants will be determined in the presence of agents that influence the proteolytic activity ..
  3. Mechanisms of innate immune response modulation by mechanical ventilation
    William A Altemeier; Fiscal Year: 2012
    ..of LPS-induced inflammation by (a) using chromatin immunoprecipitation assay to identify which protein subunits of AP-1 are recruited to target gene promoters by mechanical ventilation, (b) inhibiting AP-1 activation ..
  4. Potentiation of Glycine Receptors by Ethanol
    LUIS GERARDO AGUAYO; Fiscal Year: 2010
    ..by using receptor mutagenesis, kinetic analysis of GTP binding to G proteins, heterologous expression of G protein subunits, patch-clamp and Western blot techniques...
  5. Role of Gsalpha in Regulating Osteoblast Differentiation
    JOY Y WU; Fiscal Year: 2012
    ..Finally, Aim 3 will focus on determining the ability of osteoprogenitors to differentiate towards other mesenchymal lineages such as adipocytes, using lineage tracing in vivo and cell differentiation assays in vitro. ..
  6. Roles of Gs Alpha and interaction with Wnt signaling in regulation of adipocyte d
    Partha S Sinha; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: This study focuses on mechanisms underlying how fat cells and bone cells develop from the bone marrow. This may help improve understanding and treatment of diseases such as obesity and osteoporosis. ..
  7. Development of a Novel Vaccine Against Herpes Simplex Type 2
    DEBORAH SPECTOR; Fiscal Year: 2009
    ..S. population together with the medical and psychological impacts of the disease make HSV-2 an important candidate for the development of an effective vaccine that can prevent infection or disease. ..
  8. Establishment of sister chromatid cohesion
    Douglas E Koshland; Fiscal Year: 2013
    ..different structures are mediated by structural maintenance of chromosome (smc) complexes that consist of 4 protein subunits conserved from bacteria to man...
  9. Direct Regulation of Mitochondrial Transcription by Thyroid Hormone and Receptor
    MEGAN LYNN BESTWICK; Fiscal Year: 2012
    ..Approximately 80 protein subunits are necessary to generate the enzymatic complexes of this pathway, and the remaining protein subunits are ..
  10. Pathogen-Specific Regulation of Protein Assembly
    ALLEN BERNARD REITZ; Fiscal Year: 2010
    ..sativum (pea) PBGS hexamer because it was predicted to bind in a cavity formed by three of the individual protein subunits. This cavity is present in the inactive hexamer and not in the active octamer...
  11. Age Effects on Liver Inflammation and Injury
    Alex B Lentsch; Fiscal Year: 2013
    ..These protein subunits are involved in recruitment of ubiquitinylated substrates to the proteasome for degradation...
  12. Development of a Recombinant Subunit Vaccine for CCHF
    David E Clements; Fiscal Year: 2013
    ..In addition to vaccine development, the protein subunits produced can be used for development of diagnostic reagents, as well as targets for antiviral drug ..
  13. Immunogenicity of the Type IV Secretin System
    WENDY CATHERINE BROWN; Fiscal Year: 2012
    ..TFSS protein pairs induces significantly stronger protective immune responses as compared to individual protein subunits in an A. marginale challenge model...
  14. Campylobacter jejuni outer membrane protein vaccine
    Stuart Thompson; Fiscal Year: 2009
    ..Specific Aim 3. We will determine whether immunization of mice with conserved, purified recombinant OMPs protects against subsequent experimental C. jejuni infection. ..
  15. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2013
    ..pylori and to affect survival in a novel gastro-mimetic incubation system. ..
  16. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..interdependence of the telomerase RNA, telomerase reverse transcriptase (TERT), and several additional protein subunits. The primary objective of this proposal is to elucidate how conserved structural domains within telomerase ..
  17. Intragenic Suppressors of McCune-Albright Syndrome Mutations
    ROBIN PALS RYLAARSDAM; Fiscal Year: 2010
    ..This project aims to provide important basic science information that can be used in the rational design of drugs for McCune-Albright Syndrome and other disorders cause by G-protein defects. ..
  18. Mechanisms of Oxygen Sensing
    Navdeep S Chandel; Fiscal Year: 2013
    ..This exploratory grant will provide detailed understanding of how PHD2 is regulated during hypoxia leading to rationale molecular basis for therapeutic targeting of HIFs. ..
  19. Dynamic Interactions Between G Proteins and Calcium at SNARE Complexes
    Simon T Alford; Fiscal Year: 2013
    ..We will determine how this receptor acts and how it modulates chemical communication throughout the brain. ..
  20. Adenovirus-vectored RSV vaccine not inhibited by maternal immunity
    JASON GRAHAM GALL; Fiscal Year: 2013
    ..In phase I we will test the hypothesis that RSV-specific immune responses induced by a novel replication-deficient adenovirus vector derived from non-human adenovirus will not be inhibited by human viral immunity. ..
  21. Investigation of an anti-cancer phytochemical targeting Nrf2
    Donna D Zhang; Fiscal Year: 2013
    ..the effects of brusatol on the proteins that may enhance the activity of the E3 ligase, such as the protein subunits of the E3 ligase, as well as regulatory proteins that control the dynamic assembly/disassembly of the ligase ..
  22. Mitochondrial rhomboid function in yeast
    Donna M Gordon; Fiscal Year: 2010
    ..Putative candidates identified using both of these methods will likely include protein subunits of the Pcp1p complex, accessory proteins involved in substrate recognition, scaffolding proteins involved in ..
  23. Nicotinic receptor genes & substance abuse: Functional studies of associated SNPs
    Jerry A Stitzel; Fiscal Year: 2010
    ..Such knowledge should lead to the development of improved prevention and treatment of individuals who suffer from these disorders. ..
  24. Co-chaperone role of phosducin-like protein in G protein subunit assembly
    Barry M Willardson; Fiscal Year: 2010
    ....
  25. Mitochondrial DNA and Prostate Cancer in African American
    Keshav K Singh; Fiscal Year: 2013
    ..The entire protein coding capacity of the mitochondrial genome is dedicated to the production of 13 protein subunits. These 13 proteins constitute various subunits that make up four oxidative phosphorylation (OXPHOS) complexes ..
  26. Epigenetic Consequences of Prenatal Alcohol Exposure
    Eva E Redei; Fiscal Year: 2013
    ..Identifying the mechanisms and potential reversibility of transgenerational effects and devising novel interventions in the animal model of FAE is a major goal that could potentially be beneficial for human FAE. ..
  27. The Role of TLR4 and RSV F Protein in Immunity to RSV
    JORGE C BLANCO; Fiscal Year: 2013
    ..It is expected that at the completion of this grant, we will have identified strategies that may lead to development of a RSV vaccine and new therapeutics for mitigating the pathologic host response to RSV. ..
  28. Proteins in RNA-Based Enzymes
    Andrey S Krasilnikov; Fiscal Year: 2012
    ..The specific aims of the proposal are: 1. To determine how protein subunits of RNase MRP interact with the RNA moiety;2...
  29. Proteolytic Regulation of Troponin T &Cardiac Function
    Jian Ping Jin; Fiscal Year: 2013
    ..Cardiac muscle contraction is regulated by Ca2+ through the troponin complex that consists of three protein subunits: troponin C, troponin I, and troponin T (TnT)...
  30. Analysis of metazoan SAGA complex function in gene expression
    Jerry L Workman; Fiscal Year: 2013
    ..by applicant): The SAGA complex is a highly conserved transcriptional co-activator comprised of over 20 protein subunits. SAGA contains 2 enzymatic activities, a histone acetyltransferase and a histone deubiquitinase, interacts ..
  31. CarSpers:sperm-specific ion channels;targets for male contraceptives
    David E Clapham; Fiscal Year: 2013
    ..4 distinct genes present in all mammals, including humans, that encode 6 transmembrane-spanning ion channel protein subunits. These proteins form a sperm-specific, alkaline-activated, calcium-selective ion channel localized ..
  32. Structural Studies of Telomerase Holoenzyme
    EDWARD JOHN MIRACCO; Fiscal Year: 2013
    ..These experiments will reveal the complete structure of a telomerase holoenzyme as well as offer insight into what roles intermolecular contacts and subunit movement play in telomere catalysis. ..
  33. Expression and Function of the Alpha 7 Nicotinic Receptor in Schizophrenia
    Sherry Leonard; Fiscal Year: 2013
    ..The proposed research will contribute to development of additional 17* receptor-targeted drugs for cognition. ..
  34. Structural studies of RNase P
    ALFONSO MONDRAGON; Fiscal Year: 2013
    ..almost all organisms, from bacteria to humans, and is composed of one essential RNA subunit and one or more protein subunits. The RNA component is responsible for catalysis as it can process RNA in vitro in the absence of protein...
  35. Therapeutic Small Molecule Modulators of G beta gama Signaling
    Val S Goodfellow; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): G protein subunits play a central role in G-protein coupled receptor (GPCR)-mediated signal transduction processes...
  36. Role of the SCF/Fbxo15 ubiquitin ligase in stem cells and cancer biology
    Michele Pagano; Fiscal Year: 2012
    ..We will identify biologically significant substrates of human Fbxo15 (Specific Aim 1) and validate the Fbxo15-dependent regulation of these substrates (Specific Aim 2). ..
  37. Vaccine Potential of Nipah virus-like Particles
    PRAMILA NONE WALPITA; Fiscal Year: 2013
    ..We expect that NiV VLPs will be highly effective as vaccine. Importantly, the information gleaned from our studies will direct the development of much needed vaccines for other paramyxoviruses. ..
  38. Regulation of heterotrimeric G proteins by non-receptor activators
    GREGORY GORDON TALL; Fiscal Year: 2013
    ..asymmetric) cell division (4) define the authentic cellular interactions between the compendium of G protein subunits and Ric-8A or Ric-8B...
  39. Targeting eIF2alpha siRNA to alpha(v)beta(3) Integrin-bearing Tumor Cells
    Ching An Peng; Fiscal Year: 2010
    ....
  40. Cellular Intoxication Pathway of Cytolethal Distending Toxin
    KENNETH ALAN BRADLEY; Fiscal Year: 2013
    ..Active CDT holotoxins are heterotrimeric complexes of three protein subunits, generally encoded by three contiguous genes (cdtA, cdtB, cdtC) in a single operon...
  41. Guinea pig cytomegalovirus as a model for vaccines that target endocytic entry
    Michael A McVoy; Fiscal Year: 2010
    ..This project aims to determine if guinea pig cytomegalovirus can be used to evaluate new vaccine strategies that are designed to elicit antibodies that block epithelial cell entry. ..
  42. Animal models of membrane-targeted APP intracellular domain - Resubmission 01
    Angele Parent; Fiscal Year: 2013
    ....
  43. Theoretical study of structure-function correlations of Aquaporin V and mutants
    LIAO Y CHEN; Fiscal Year: 2013
    ..The knowledge so gained will positively impact on finding new medicines to prevent and control AQP5-related diseases. ..
  44. G Protein Modulation of Glycine Receptor Function and Ethanol Action
    Gregg E Homanics; Fiscal Year: 2012
    ..Our primary objectives are to generate gene knockin mice that harbor mutant a1 GlyRs that are insensitive to modulation by G???, and test these mice for cellular and behavioral responses to EtOH. ..
  45. Innate and acquired immune responses to novel pneumococcal T cell antigens
    KRISTIN LEIGH MOFFITT; Fiscal Year: 2013
    ..Further characterization of candidate proteins and elucidation of the mechanism of immunity they elicit are critical to advance the development of a protein subunit vaccine that prevents colonization. ..
  46. Structure and Dynamics of Gap Junction Channels
    MARK JAY YEAGER; Fiscal Year: 2013
    ..is accomplished by a unique set of molecular pores called gap junction channels, which are assembled from protein subunits called connexins...
  47. Molecular Pathogenesis of Diamond Blackfan Anemia
    KATHLEEN MIHO SAKAMOTO; Fiscal Year: 2013
    ..Mutations in ribosomal protein subunits (RPS) 19 and 11 have been described...
  48. Depots of persistent influenza antigens support protective CD4 T cell memory
    Dawn M Jelley Gibbs; Fiscal Year: 2010
    ....
  49. Preweanling Methylphenidate Exposure and Reward Behavior
    Cynthia Crawford; Fiscal Year: 2009
    ..D2 receptor functioning will be assessed by measuring adenylyl cyclase activity, G-protein subunits levels (i.e...
  50. Assembly of type IV collagen in health and disease
    Raghu Kalluri; Fiscal Year: 2013
    ..Such studies will provide further insights into possible cell based therapy options for Alport syndrome, and will also provide a basic understanding of type IV collagen assembly in the GBM. ..
  51. SYNAPTIC MECHANISMS IN RETINA
    Malcolm M Slaughter; Fiscal Year: 2012
    ..We hypothesize that these newly discovered receptors can influence how the normal retina functions and how that might change in diabetic conditions. We will also explore a new glycine receptor that controls inhibition in the retina. ..
  52. Development of stable and rapidly acting adjuvanted vaccines for biodefense
    ROBERT NEWTON BREY; Fiscal Year: 2013
    DESCRIPTION: (provided by the applicant) With the current emphasis on defined vaccines composed of protein subunits, there is a persistent need to improve the stability and performance of vaccines...
  53. Outside-in signaling mechanisms of platelet integrin alpha-llb-beta3
    Xiaoping Du; Fiscal Year: 2013
    ....
  54. Structural Studies of Eukaryotic Transcription
    Francisco J Asturias; Fiscal Year: 2012
    ..a cryo-EM structure of Mediator with high enough resolution to identify density corresponding to individual protein subunits. 3D difference mapping and molecular labeling techniques will be used to localize specific subunits and ..
  55. Collagen IV Networks of Basement Membranes
    ROBERTO MARCELO VANACORE; Fiscal Year: 2013
    ..This new information may lead to the discovery of novel molecular targets for the development of new alternative therapies for the treatment of DN. ..
  56. BIOPHYSICS OF RECEPTOR/G-PROTEIN INTERACTIONS
    Oleg G Kisselev; Fiscal Year: 2011
    ..interface, the mechanism of signal transfer from the receptor, and the roles of individual G- protein subunits, especially the G23 subunit complex, in this dynamic process...
  57. Regulation of the Tumor Microenvironment by KSHV
    Christopher H Parsons; Fiscal Year: 2013
    ..Therefore, understanding mechanisms for how KSHV regulates expression of its own receptors and the longevity of KSHV-infected cells could provide a scientific basis for developing novel preventive and therapeutic targets for KS. ..
  58. Novel Mechanisms Regulating G Protein and MAP Kinase Signaling
    Yuqi Wang; Fiscal Year: 2010
    ..The main hypothesis is that G protein signaling is regulated via ubiquitination of G protein subunits. The hypothesis is based on recent observations in yeast that both G( subunit (Gpa1) and G( subunit (Ste4) ..
  59. Telomeres, Telomerase and Tumor Progression
    Steven E Artandi; Fiscal Year: 2013
    ....
  60. Synergism of telomere maintenance and telomerase with cancer-promoting signaling
    Elizabeth H Blackburn; Fiscal Year: 2013
    ..This research is anticipated to generate new approaches for anti-cancer therapies. ..
  61. Structure and Function of the proton-turbine of the ATP-Synthase
    Petra Fromme; Fiscal Year: 2010
    ..While several structures of the membrane extrinsic F1 part and individual protein subunits of the ion conduction FO part have been determined by X-ray structure crystallography, the molecular ..
  62. G PROTEIN COUPLED RECEPTOR ACTIVATION STUDIED IN YEAST
    Mark Dumont; Fiscal Year: 2009
    ..the two types of receptors and G proteins, a genetic screen will be conducted to identify mutations in G protein subunits that specifically prevent the interaction with receptors and mutations in receptors that specifically ..
  63. Function and Expression of Connexins in the pre-Botzinger Complex
    JONATHAN KELTY; Fiscal Year: 2007
    ..These trans-cellular channels are made up of joined hemichannels, each a hexamer of integral membrane protein subunits termed connexins (Cx)...
  64. Adjuvants for Agile Vaccine Development
    Philip Felgner; Fiscal Year: 2006
    ..abstract_text> ..
  65. INACTIVATION OF THE PIGA GENE IN HEMATOPOIETIC STEM CELL
    Monica Bessler; Fiscal Year: 2002
    ..The findings could provide the scientific foundation of new therapeutic modalities for PNH and other bone marrow failure syndromes. ..
  66. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2006
    ..2) Determine functional and physical relationships between G protein subunits. The mechanism of posttranscriptional regulation of G-alpha levels by GNB-1 will be determined using pulse-..
  67. MODULATION OF SODIUM CHANNELS BY G PROTEINS
    Todd Scheuer; Fiscal Year: 2001
    ..demonstrate that directly activating G proteins with guanine nucleotides or by expression of recombinant G protein subunits results in strong sodium channel modulation...
  68. Cyclic GMP Phosphodiesterase in Signaling
    Hsien Yu Wang; Fiscal Year: 2007
    ..observations provide the basis for the specific aims in this proposal: suppression of the heterotrimeric G-protein subunits G(alpha)t2/o in mouse F9 teratocarcinoma stem cells and treatment with inhibitors of cyclic GMP PDE, such as ..
  69. STRUCTURAL STUDIES OF PROTEIN SUBUNITS
    Thomas Smith; Fiscal Year: 2004
    ..These studies have the potential of changing the way we view the early steps in the viral infection process and will allow us to develop tools that are applicable to other viral systems. ..
  70. Transcriptional control of FSH expression
    Daniel Bernard; Fiscal Year: 2007
    ..Activins, produced within gonadotropes, stimulate the production of one of the two protein subunits that comprise FSH, the FSHbeta subunit...
  71. STRUCTURE, SYNTHESIS AND ASSEMBLY OF PICORNAVIRUSES
    ROLAND RUECKERT; Fiscal Year: 1991
    ..Selected mutants will be analyzed by x-ray crystallography at Purdue University to determine the effect of the mutation on the 3-dimensional structure of the pocket...
  72. Chemosensing in the Gastrointestinal Tract
    Catia Sternini; Fiscal Year: 2010
    ..The recent discovery that bitter taste receptors (T2Rs) and the G-protein subunits, ??gustducin and ??transducin, which mediate gustatory signals in the oral cavity, are also expressed in the ..
  73. ROLE OF THE PNH PHENOTYPE IN LEUKEMIC TRANSFORMATION
    Monica Bessler; Fiscal Year: 2005
    ..The availability of a mouse model for PNH will provide us with a powerful tool to test new therapeutic agents for the treatment of PNH, PNH/MDS, PNH,AML and possibly other clonal blood disorders. ..