gag gene products

Summary

Summary: Proteins coded by the retroviral gag gene. The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. Many of the final products are associated with the nucleoprotein core of the virion. gag is short for group-specific antigen.

Top Publications

  1. ncbi Lentiviral RNAs can use different mechanisms for translation initiation
    Emiliano P Ricci
    Unité de Virologie Humaine, Ecole Normale Superieure de Lyon, IFR 128, Lyon, F 69364, France
    Biochem Soc Trans 36:690-3. 2008
  2. ncbi CD8+ T-cell responses to different HIV proteins have discordant associations with viral load
    Photini Kiepiela
    HIV Pathogenesis Programme, Doris Duke Medical Research Institute, University of KwaZulu Natal, Durban 4013, South Africa
    Nat Med 13:46-53. 2007
  3. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
  4. pmc Superior control of HIV-1 replication by CD8+ T cells is reflected by their avidity, polyfunctionality, and clonal turnover
    Jorge R Almeida
    Cellular Immunology Laboratory, U543, Institut National de la Sante et de la Recherche Medicale, Avenir Group, 75013 Paris, France
    J Exp Med 204:2473-85. 2007
  5. ncbi The protein network of HIV budding
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Cell 114:701-13. 2003
  6. ncbi The stoichiometry of Gag protein in HIV-1
    John A G Briggs
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK
    Nat Struct Mol Biol 11:672-5. 2004
  7. ncbi Gag-specific CD8+ T lymphocytes recognize infected cells before AIDS-virus integration and viral protein expression
    Jonah B Sacha
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53711, USA
    J Immunol 178:2746-54. 2007
  8. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
  9. pmc Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function
    Dimiter G Demirov
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 99:955-60. 2002
  10. ncbi AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly
    Xinhong Dong
    Department of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    Cell 120:663-74. 2005

Research Grants

Detail Information

Publications263 found, 100 shown here

  1. ncbi Lentiviral RNAs can use different mechanisms for translation initiation
    Emiliano P Ricci
    Unité de Virologie Humaine, Ecole Normale Superieure de Lyon, IFR 128, Lyon, F 69364, France
    Biochem Soc Trans 36:690-3. 2008
    ..Our results show that HIV-1 is able to drive the synthesis of the Gag polyprotein both by a classical cap-dependent mechanism and an IRES, whereas HIV-2 and SIV appear to use exclusively an IRES mechanism...
  2. ncbi CD8+ T-cell responses to different HIV proteins have discordant associations with viral load
    Photini Kiepiela
    HIV Pathogenesis Programme, Doris Duke Medical Research Institute, University of KwaZulu Natal, Durban 4013, South Africa
    Nat Med 13:46-53. 2007
    ..These population-based data, suggesting the existence of both effective immune responses and responses lacking demonstrable biological impact in chronic HIV infection, are of relevance to HIV vaccine design and evaluation...
  3. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  4. pmc Superior control of HIV-1 replication by CD8+ T cells is reflected by their avidity, polyfunctionality, and clonal turnover
    Jorge R Almeida
    Cellular Immunology Laboratory, U543, Institut National de la Sante et de la Recherche Medicale, Avenir Group, 75013 Paris, France
    J Exp Med 204:2473-85. 2007
    ..Such attributes are interlinked and constitute the basis for effective control of HIV-1 replication. These data on the features of effective CD8+ T cells in HIV infection may aid in the development of successful T cell vaccines...
  5. ncbi The protein network of HIV budding
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Cell 114:701-13. 2003
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses...
  6. ncbi The stoichiometry of Gag protein in HIV-1
    John A G Briggs
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK
    Nat Struct Mol Biol 11:672-5. 2004
    ..In the immature virus, Gag forms a hexameric lattice with a spacing of 8.0 nm. Thus, less than half of the CA proteins form the mature core...
  7. ncbi Gag-specific CD8+ T lymphocytes recognize infected cells before AIDS-virus integration and viral protein expression
    Jonah B Sacha
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53711, USA
    J Immunol 178:2746-54. 2007
    ..0017) in SIV-infected rhesus macaques. These results suggest that HIV vaccines should focus CD8(+) T cell responses on Gag...
  8. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies...
  9. pmc Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function
    Dimiter G Demirov
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 99:955-60. 2002
    ..These data demonstrate a link between the E2-like domain of TSG101 and HIV-1 L domain function, and indicate that TSG101 derivatives can act as potent and specific inhibitors of HIV-1 replication by blocking virus budding...
  10. ncbi AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly
    Xinhong Dong
    Department of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    Cell 120:663-74. 2005
    ..These studies illuminate an early step in retroviral particle assembly and provide evidence that the trafficking of Gag to late endosomes is part of a productive particle assembly pathway...
  11. pmc Magnitude of functional CD8+ T-cell responses to the gag protein of human immunodeficiency virus type 1 correlates inversely with viral load in plasma
    Bradley H Edwards
    Department of Medicine, University of Alabama, 845 19th Street South, BBRB 226, Birmingham, AL 35294, USA
    J Virol 76:2298-305. 2002
    ..These data serve as strong evidence that major histocompatibility complex class I presentation of Gag peptides is an essential feature for any HIV-1 vaccine designed to elicit optimal CD8+ T-cell responses...
  12. pmc Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly
    Jamil S Saad
    Howard Hughes Medical Institute, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    Proc Natl Acad Sci U S A 103:11364-9. 2006
    ..Our findings indicate that PI(4,5)P(2) acts as both a trigger of the myristyl switch and a membrane anchor and suggest a potential mechanism for targeting Gag to membrane rafts...
  13. ncbi Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding
    Robert D Fisher
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Cell 128:841-52. 2007
    ..ALIX therefore serves as a flexible, extended scaffold that connects retroviral Gag proteins to ESCRT-III and other cellular-budding machinery...
  14. ncbi HIV-1 viral escape in infancy followed by emergence of a variant-specific CTL response
    Margaret E Feeney
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    J Immunol 174:7524-30. 2005
    ..Taken together, these findings indicate that the developing immune system of children may exhibit greater plasticity in responding to a continually evolving chronic viral infection...
  15. pmc Virus population homogenization following acute human immunodeficiency virus type 1 infection
    Gerald H Learn
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    J Virol 76:11953-9. 2002
    ..This early selective process focuses on viral properties within Env but not Gag p17. Hence, the viral homogeneity observed early in HIV-1 infection results from a selective process that occurs during the establishment of infection...
  16. pmc In vitro assembly properties of human immunodeficiency virus type 1 Gag protein lacking the p6 domain
    S Campbell
    ABL Basic Research Program, NCI Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA
    J Virol 73:2270-9. 1999
    ....
  17. ncbi Visualization of retroviral replication in living cells reveals budding into multivesicular bodies
    Nathan M Sherer
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Ave, New Haven, CT 06536, USA
    Traffic 4:785-801. 2003
    ..These data suggest that retroviruses can interact with the vps sorting machinery in a more traditional sense, directly linked to the mechanism by which cellular proteins are sorted into multivesicular endosomes...
  18. pmc Plasma membrane rafts play a critical role in HIV-1 assembly and release
    A Ono
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Room 307, 9000 Rockville Pike, Bethesda, MD 20892-0460, USA
    Proc Natl Acad Sci U S A 98:13925-30. 2001
    ..These results identify the association of Gag with plasma membrane rafts as an important step in HIV-1 replication. These findings may lead to novel strategies for suppressing HIV-1 replication in vivo...
  19. ncbi Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2
    Melissa Batonick
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Virology 342:190-200. 2005
    ..Together these data attribute a role to the AP-2 complex in the regulation of HIV-1 assembly/release...
  20. pmc HLA-associated immune escape pathways in HIV-1 subtype B Gag, Pol and Nef proteins
    Zabrina L Brumme
    Ragon Institute of MGH, MIT and Harvard, Charlestown, MA, USA
    PLoS ONE 4:e6687. 2009
    ..Results are organized into protein-wide escape maps illustrating the sites and pathways of HLA-driven viral evolution...
  21. pmc Mutations of the human immunodeficiency virus type 1 p6Gag domain result in reduced retention of Pol proteins during virus assembly
    X F Yu
    Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    J Virol 72:3412-7. 1998
    ..These results suggest that the p6 domain of HIV-1 Gag may play an important role in recruiting or retaining cleaved Pol proteins during virus assembly...
  22. ncbi HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
    J Martin-Serrano
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
    Nat Med 7:1313-9. 2001
    ..Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens...
  23. pmc Functional replacement and positional dependence of homologous and heterologous L domains in equine infectious anemia virus replication
    Feng Li
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Virol 76:1569-77. 2002
    ....
  24. pmc The late domain of human immunodeficiency virus type 1 p6 promotes virus release in a cell type-dependent manner
    Dimiter G Demirov
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Virol 76:105-17. 2002
    ..The results described here define the role of p6 in virus replication in a wide range of cell types and reveal a strong cell type-dependent requirement for p6 in virus particle budding...
  25. pmc Improved primate foamy virus vectors and packaging constructs
    Martin Heinkelein
    Institut fur Virologie und Immunbiologie, Universitat Wurzburg, Germany
    J Virol 76:3774-83. 2002
    ..By this strategy, efficient vector transfer was achieved with constructs that have minimal genetic overlap...
  26. pmc Tsg101 control of human immunodeficiency virus type 1 Gag trafficking and release
    A Goff
    Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794 5222, USA
    J Virol 77:9173-82. 2003
    ....
  27. pmc Characterization of prototype foamy virus gag late assembly domain motifs and their role in particle egress and infectivity
    Annett Stange
    Institut fur Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Fetscherstr 74, 01307 Dresden, Germany
    J Virol 79:5466-76. 2005
    ..Taken together these results demonstrate that PFV, like other viruses, requires components of the vacuolar protein sorting (VPS) machinery for egress and enters the VPS pathway through interaction with TSG101...
  28. pmc T cell cross-reactivity and conformational changes during TCR engagement
    Jean K Lee
    Human Immunology Unit, Medical Research Council, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England, UK
    J Exp Med 200:1455-66. 2004
    ..These findings have implications for the rational design of vaccines targeting viruses with unstable genomes...
  29. ncbi The M-PMV cytoplasmic targeting-retention signal directs nascent Gag polypeptides to a pericentriolar region of the cell
    Jeffrey N Sfakianos
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Traffic 4:660-70. 2003
    ..Thus the previously defined cytoplasmic targeting-retention signal appears to act as a cotranslational intracellular targeting signal that concentrates Gag proteins at the centriole for assembly of capsids...
  30. ncbi Intracellular versus cell surface assembly of retroviral pseudotypes is determined by the cellular localization of the viral glycoprotein, its capacity to interact with Gag, and the expression of the Nef protein
    Virginie Sandrin
    INSERM U412, Lyon Ecole Normale Supérieure de Lyon, and IFR128 BioSciences Lyon Gerland, Lyon, F 69007 France
    J Biol Chem 281:528-42. 2006
    ..Finally, the expression of Nef proteins specifically enhanced the incorporation of the retroviral GPs by increasing their localization in late endosomes...
  31. pmc Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane
    Amy M Booth
    Department of Biological Chemistry and 2Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biol 172:923-35. 2006
    ..In support of this, we find that Jurkat T cells direct the key budding factor of HIV, HIV Gag, to these endosome-like domains of plasma membrane and secrete HIV Gag from the cell in exosomes...
  32. pmc HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein
    Owen Pornillos
    Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT 84132, USA
    J Cell Biol 162:425-34. 2003
    ..HIV-1 Gag apparently mimics this Hrs activity, and thereby usurps Tsg101 and other components of the MVB vesicle fission machinery to facilitate viral budding...
  33. pmc Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release
    H G Gottlinger
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 88:3195-9. 1991
    ..Inhibition of the release of the viral capsid proteins by interferon alpha indicates that this step of virus maturation may be sensitive to inhibition by antiviral drugs...
  34. ncbi HIV-1 nucleocapsid protein as a nucleic acid chaperone: spectroscopic study of its helix-destabilizing properties, structural binding specificity, and annealing activity
    Maria A Urbaneja
    AIDS Vaccine Program, SAIC Frederick, Building 535 424, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Mol Biol 318:749-64. 2002
    ....
  35. ncbi Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid
    T R Gamble
    Biochemistry Department, University of Utah, Salt Lake City 84103, USA
    Cell 87:1285-94. 1996
    ..Side by side association of these strips may allow capsid to form the surface of the viral core. Cyclophilin A could then function by weakening the association between capsid strips, thereby promoting disassembly of the viral core...
  36. pmc The efficacy of DNA vaccination is enhanced in mice by targeting the encoded protein to dendritic cells
    Godwin Nchinda
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, New York 10065, USA
    J Clin Invest 118:1427-36. 2008
    ..The efficacy of DNA vaccines therefore may be enhanced by inclusion of sequences such as single-chain antibodies to target the antigen to DCs...
  37. pmc Proteins related to the Nedd4 family of ubiquitin protein ligases interact with the L domain of Rous sarcoma virus and are required for gag budding from cells
    A Kikonyogo
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 98:11199-204. 2001
    ..These findings mechanistically link the cellular ubiquitination pathway to retrovirus budding...
  38. pmc The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their budding
    Gregory Camus
    Institut Cochin, Universite Paris Descartes, Centre National de la Recherche Scientifique UMR 8104, Paris, France
    Mol Biol Cell 18:3193-203. 2007
    ..We propose that AP-1 promotes Gag release by transporting it to intracellular sites of active budding, and/or by facilitating its interactions with other cellular partners...
  39. pmc Transactivation of elements in the human endogenous retrovirus W family by viral infection
    Christoffer Nellåker
    The Department of Neuroscience, Karolinska Institutet, Retzius vag 8, 171 77 Stockholm, Sweden
    Retrovirology 3:44. 2006
    ....
  40. pmc Contribution of CD8+ T cells to containment of viral replication and emergence of mutations in Mamu-A*01-restricted epitopes in Simian immunodeficiency virus-infected rhesus monkeys
    Eun Young Kim
    Division of Infectious Diseases, The Feinberg School of Medicine, Northwestern University, 676 N St Clair St, Suite 200, Chicago, IL 60611, USA
    J Virol 82:5631-5. 2008
    ..These results confirm the importance of cytotoxic T cells in controlling viremia and the constraint on epitope sequences that require compensatory changes to go to fixation...
  41. pmc Infectious HIV-1 assembles in late endosomes in primary macrophages
    Annegret Pelchen-Matthews
    Cell Biology Unit, Medical Research MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK
    J Cell Biol 162:443-55. 2003
    ..This notion has significant implications for understanding the biology of HIV and its cell-cell transmission...
  42. ncbi Rev-independent expression of synthetic gag-pol genes of human immunodeficiency virus type 1 and simian immunodeficiency virus: implications for the safety of lentiviral vectors
    R Wagner
    Institut für medizinische Mikrobiologie and Hygiene, Universitat Regensburg, D 93053 Regensburg, Germany
    Hum Gene Ther 11:2403-13. 2000
    ..By eliminating regions of homology and sequences involved in packaging, synthetic gag-pol genes should improve the safety profile of lentiviral vectors...
  43. ncbi On-going generation of multiple forms of HIV-1 intersubtype recombinants in the Yunnan Province of China
    Rongge Yang
    Laboratory of Molecular Virology and Epidemiology, AIDS Research Center, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    AIDS 16:1401-7. 2002
    ....
  44. pmc Organization of immature human immunodeficiency virus type 1
    T Wilk
    The Structural Biology Programme, European Molecular Biology Laboratory, D69012 Heidelberg, Federal Republic of Germany
    J Virol 75:759-71. 2001
    ..Authentic, immature HIV-1 displays additional surface features and an increased density between the lipid bilayers which reflect the presence of gp41. The other internal features match those of virus-like particles...
  45. ncbi HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRET
    Emma Poole
    Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, CB2 2QQ, UK
    Traffic 6:741-55. 2005
    ..HIV-1 Psi thus acts as a subcellular localisation signal as well as a high-affinity-binding site for Gag...
  46. ncbi Characterization and selection of HIV-1 subtype C isolates for use in vaccine development
    Carolyn Williamson
    Division of Medical Virology, University of Cape Town, Observatory, Cape Town, South Africa 7925
    AIDS Res Hum Retroviruses 19:133-44. 2003
    ..Genes identified in this study have been incorporated into the VEE VRP candidate vaccines targeted for clinical trial in South Africa...
  47. ncbi Identification of a host protein essential for assembly of immature HIV-1 capsids
    Concepcion Zimmerman
    Department of Physiology, University of California at San Francisco, San Francisco, California 94143, USA
    Nature 415:88-92. 2002
    ..These findings support a critical role for HP68 in post-translational events of HIV-1 assembly and reveal a previously unappreciated dimension of host-viral interaction...
  48. ncbi Proteolytic processing of foamy virus Gag and Pol proteins
    R M Flugel
    Retroviral Gene Expression, Research Programme Applied Tumor Virology, German Cancer Research Center, Im Neuenheimer Feld 242, 69009 Heidelberg, Germany
    Curr Top Microbiol Immunol 277:63-88. 2003
    ..The temporal and spatial control and the factors that regulate FV PRs remain to be elucidated...
  49. pmc PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101 [corrected]
    Fadila Bouamr
    Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York, USA
    J Virol 77:11882-95. 2003
    ..This result suggests that Nedd4 is involved early in budding of HTLV-1...
  50. pmc Virus maturation by budding
    H Garoff
    Department of Biosciences at Novum, S 141 57 Huddinge, Sweden
    Microbiol Mol Biol Rev 62:1171-90. 1998
    ..This new concept also provides answers to the question of how viral and cellular membrane proteins are sorted during budding. In addition, it has implications for the mechanism by which the virion is uncoated during virus entry...
  51. pmc Interaction of the human immunodeficiency virus type 1 nucleocapsid with actin
    B Liu
    Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    J Virol 73:2901-8. 1999
    ....
  52. pmc Entropic switch regulates myristate exposure in the HIV-1 matrix protein
    Chun Tang
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 5398, USA
    Proc Natl Acad Sci U S A 101:517-22. 2004
    ..Our findings indicate that the HIV-1 myristyl switch is regulated not by mechanically induced conformational changes, as observed for other myristyl switches, but instead by entropic modulation of a preexisting equilibrium...
  53. pmc The microbial mimic poly IC induces durable and protective CD4+ T cell immunity together with a dendritic cell targeted vaccine
    Christine Trumpfheller
    Laboratory of Cellular Physiology and Immunology, Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:2574-9. 2008
    ..We suggest that poly IC be tested as an adjuvant with DC-targeted vaccines to induce numerous multifunctional CD4(+) Th1 cells with proliferative capacity...
  54. ncbi Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia
    Y Yao
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Genes Brain Behav 7:103-12. 2008
    ..05) in the patients compared with the controls. Thus, studies aiming to further understanding of complex human disease such as schizophrenia may need to be extended beyond the strictly protein-coding fraction of the transcriptome...
  55. pmc APOBEC3G incorporation into human immunodeficiency virus type 1 particles
    Veronique Zennou
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, NY 10016, USA
    J Virol 78:12058-61. 2004
    ..Because it is, therefore, difficult to evolve specific sequences that confer escape from APOBEC3G, these findings may explain why lentiviruses evolved an activity that induces its destruction...
  56. ncbi M-PMV capsid transport is mediated by Env/Gag interactions at the pericentriolar recycling endosome
    Jeffrey N Sfakianos
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Traffic 4:671-80. 2003
    ..Thus, for the first time, endocytic trafficking of a retroviral Env glycoprotein is linked to the efficient cytoplasmic transport of Gag...
  57. pmc Sequence-specific binding of human immunodeficiency virus type 1 nucleocapsid protein to short oligonucleotides
    R J Fisher
    Protein Chemistry Laboratory, SAIC Frederick, NCI Frederick Cancer Research and Development Center, Maryland 21702, USA
    J Virol 72:1902-9. 1998
    ....
  58. ncbi Nedd4.1-mediated ubiquitination and subsequent recruitment of Tsg101 ensure HTLV-1 Gag trafficking towards the multivesicular body pathway prior to virus budding
    Vincent Blot
    Departement de Biologie Cellulaire, CNRS UMR 8104 and INSERM U567, Institut Cochin, 75014 Paris, France
    J Cell Sci 117:2357-67. 2004
    ..Our findings indicate that Nedd4.1 and Tsg101 act successively in the assembly process of HTLV-1 to ensure proper Gag trafficking through the endocytic pathway up to late endosomes where the late steps of retroviral release occur...
  59. pmc Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1
    Luciana J Costa
    Molecular Virology Laboratory, Dep of Genetics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    Retrovirology 3:33. 2006
    ..In this report, we investigated further the ability of Nef to facilitate the replication of HIV-1...
  60. ncbi Heteronuclear NMR studies of the interaction of tRNA(Lys)3 with HIV-1 nucleocapsid protein
    C Tisne
    Laboratoire de Cristallographie et RMN Biologiques, EP 2075 CNRS Faculté de Pharmacie, 4 avenue de l Observatoire, Paris, 75006, France
    J Mol Biol 306:443-54. 2001
    ..It is interesting that for the biological role of the NC protein, these pairs could be the starting points of the tRNA melting required for the hybridisation to the viral RNA...
  61. ncbi Human endogenous retrovirus (HERV)-W ENV and GAG proteins: physiological expression in human brain and pathophysiological modulation in multiple sclerosis lesions
    Hervé Perron
    bioMerieux, R and D, Chemin de l orme, 69280 Marcy l Etoile, France
    J Neurovirol 11:23-33. 2005
    ..This is compatible with a pathophysiological role in MS, but also illustrates the ambivalence of such HERV antigens, which can be expressed in cell-specific patterns, under physiological or pathological conditions...
  62. pmc Role for HLA class II molecules in HIV-1 suppression and cellular immunity following antiretroviral treatment
    U Malhotra
    Program in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D3-100, Seattle, WA 98109, USA
    J Clin Invest 107:505-17. 2001
    ..Eliciting responses to the promiscuous epitope region may be beneficial in vaccine strategies...
  63. pmc Efficient processing of the immunodominant, HLA-A*0201-restricted human immunodeficiency virus type 1 cytotoxic T-lymphocyte epitope despite multiple variations in the epitope flanking sequences
    C Brander
    AIDS Research Center and Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Virol 73:10191-8. 1999
    ..These data suggest that escape from this immunodominant CTL response is not frequently accomplished by changes in the epitope flanking sequences...
  64. pmc Escape in one of two cytotoxic T-lymphocyte epitopes bound by a high-frequency major histocompatibility complex class I molecule, Mamu-A*02: a paradigm for virus evolution and persistence?
    Thorsten U Vogel
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Virol 76:11623-36. 2002
    ..Differential selection by CTL may therefore be a paradigm of immunodeficiency virus infection...
  65. ncbi Regulation of human endogenous retrovirus W protein expression by herpes simplex virus type 1: implications for multiple sclerosis
    Klemens Ruprecht
    Department of Neurology, Clinical Research Unit for Multiple Sclerosis and Neuroimmunology, Wurzburg, Germany
    J Neurovirol 12:65-71. 2006
    ....
  66. pmc Evidence for a new viral late-domain core sequence, FPIV, necessary for budding of a paramyxovirus
    Anthony P Schmitt
    Northwestern University, 2205 Tech Dr, Evanston, IL 60208 3500, USA
    J Virol 79:2988-97. 2005
    ..We hypothesize that these proline residues act to partially restore virus budding by generation of new motifs that act as suboptimal late domains...
  67. ncbi Evaluation of minor groove binding probe and Taqman probe PCR assays: Influence of mismatches and template complexity on quantification
    Yuanrong Yao
    Department of Neuroscience, Karolinska Institute, Retzius vag 8, S 171 77 Stockholm, Sweden
    Mol Cell Probes 20:311-6. 2006
    ....
  68. pmc A role for ubiquitin ligase recruitment in retrovirus release
    B Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:13063-8. 2000
    ..Our findings suggest that the engagement of the ubiquitin conjugation machinery by L domains plays a crucial role in the release of a diverse group of enveloped viruses...
  69. ncbi Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes
    J E Schmitz
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Science 283:857-60. 1999
    ..These results confirm the importance of cell-mediated immunity in controlling HIV-1 infection and support the exploration of vaccination approaches for preventing infection that will elicit these immune responses...
  70. pmc Nucleic acid-independent retrovirus assembly can be driven by dimerization
    Marc C Johnson
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    J Virol 76:11177-85. 2002
    ....
  71. ncbi Studies on the mechanism of inactivation of the HIV-1 nucleocapsid protein NCp7 with 2-mercaptobenzamide thioesters
    Lisa M Miller Jenkins
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Med Chem 48:2847-58. 2005
    ..These studies provide a better understanding of the mechanism of action of thioester compounds, which is important for future design of anti-HIV-1 compounds that target NCp7...
  72. pmc Role of murine leukemia virus nucleocapsid protein in virus assembly
    Delphine Muriaux
    HIV Drug Resistance Program, National Cancer Institute Frederick, P O Box B, Frederick, MD 21702 1201, USA
    J Virol 78:12378-85. 2004
    ..Therefore, the functions of NC in virus budding and infectivity are completely distinct from viral late-domain function...
  73. pmc Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15
    Atsushi Okumura
    The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:1440-5. 2006
    ..Identification of ISG15 as the critical component in IFN-mediated inhibition of HIV-1 release advances the understanding of the IFN-mediated inhibition of HIV-1 replication and uncovers a target for the anti HIV-1 therapy...
  74. ncbi Human immunodeficiency virus type 1 assembly, release, and maturation
    Catherine S Adamson
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Adv Pharmacol 55:347-87. 2007
  75. ncbi Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity
    John W Shiver
    Merck Research Laboratories, West Point, Pennsylvania 19486, USA
    Nature 415:331-5. 2002
    ..The replication-defective adenovirus is a promising vaccine vector for development of an HIV-1 vaccine...
  76. ncbi Affinities of the nucleocapsid protein for variants of SL3 RNA in HIV-1
    Andrew C Paoletti
    Department of Chemistry, Graduate Program in Structural Biology, Biochemistry, and Biophysics, Syracuse University, Syracuse, New York 13244 4100, USA
    Biochemistry 41:15423-8. 2002
    ..These results are interesting in the context of RNA-protein interaction, as well as for the discovery of antiNC agents for AIDS therapy...
  77. ncbi Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B
    J Luban
    Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032
    Cell 73:1067-78. 1993
    ..The Gag-CyP interaction may be important for the HIV-1 life cycle and may be relevant to the pathology caused by this immunosuppressive virus...
  78. pmc Murine leukemia virus particle assembly quantitated by fluorescence microscopy: role of Gag-Gag interactions and membrane association
    Mariam Andrawiss
    Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Science, University College London, London, United Kingdom
    J Virol 77:11651-60. 2003
    ..These data suggest that two independent mechanisms, CA interactions and membrane association following myristylation, cooperate in MLV Gag assembly and budding...
  79. pmc Transmission of HIV-1 Gag immune escape mutations is associated with reduced viral load in linked recipients
    Paul A Goepfert
    Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Exp Med 205:1009-17. 2008
    ..In addition to their direct implications for HIV-1 vaccine design, these data suggest that CTL-induced viral polymorphisms and their associated in vivo viral fitness costs could have a significant impact on HIV-1 pathogenesis...
  80. ncbi Functional relationship between the matrix proteins of feline and simian immunodeficiency viruses
    Mariana L Manrique
    Centro de Virologia Animal CEVAN CONICET, C1414DEM Buenos Aires, Argentina
    Virology 329:157-67. 2004
    ..Our results thus provide novel information about the functional homology between the MA proteins of distantly related lentiviruses...
  81. ncbi Mutational analysis of the feline immunodeficiency virus matrix protein
    M L Manrique
    , Serrano 669, C1414DEM, Buenos Aires, Argentina
    Virus Res 76:103-13. 2001
    ..Our results reveal the role that the FIV MA plays in virus morphogenesis and contribute to the understanding of the assembly process in non-primate lentiviruses...
  82. pmc The late-domain-containing protein p6 is the predominant phosphoprotein of human immunodeficiency virus type 1 particles
    Barbara Muller
    Abteilung Virologie, Universitatsklinikum Heidelberg, D 69120 Heidelberg, Germany
    J Virol 76:1015-24. 2002
    ..Inhibition experiments suggested that a cyclin-dependent kinase or a related kinase, most likely ERK2, was involved in p6 phosphorylation by virion-associated enzymes...
  83. pmc Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag)
    L VerPlank
    Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, NY 11794 5222, USA
    Proc Natl Acad Sci U S A 98:7724-9. 2001
    ..The results link L-domain function in HIV to the Ub machinery and a specific component of the cellular trafficking apparatus...
  84. pmc Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 101:14889-94. 2004
    ..These results demonstrate that PI(4,5)P2 plays a key role in Gag targeting to the plasma membrane and thus serves as a cellular determinant of HIV-1 particle production...
  85. ncbi How retroviruses select their genomes
    Victoria D'Souza
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA
    Nat Rev Microbiol 3:643-55. 2005
    ....
  86. ncbi Mechanism of nucleocapsid protein catalyzed structural isomerization of the dimerization initiation site of HIV-1
    Manuela J Rist
    Center for Advanced Research in Biotechnology of the University of Maryland, Rockville 20850, USA
    Biochemistry 41:14762-70. 2002
    ....
  87. ncbi Dendritic cell-lysosomal-associated membrane protein (LAMP) and LAMP-1-HIV-1 gag chimeras have distinct cellular trafficking pathways and prime T and B cell responses to a diverse repertoire of epitopes
    Luciana B Arruda
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    J Immunol 177:2265-75. 2006
    ..These data indicate that LAMP-1 and DC-LAMP Ag chimeras follow different trafficking pathways, induce distinct modulatory immune responses, and are able to present cryptic epitopes...
  88. ncbi Retroviral genomic RNAs are transported to the plasma membrane by endosomal vesicles
    Eugenia Basyuk
    IGMM CNRS UMR5535, Universite Montpellier II, IFR 24, 1919, route de Mende, 34293 Cedex 5, Montpellier, France
    Dev Cell 5:161-74. 2003
    ..This may allow retroviruses to hijack the endosomal machinery as part of their biosynthetic pathway. More generally, tethering to vesicles may provide an efficient mechanism for directed RNA transport...
  89. ncbi HIV-1 Gag protein associates with F-actin present in microfilaments
    O Rey
    Department of Pediatrics, School of Medicine, University of California at Los Angeles 90095, USA
    Virology 220:530-4. 1996
    ..In vivo and in vitro analyses of this interaction indicated that the unprocessed Gag polyprotein is capable of association with polymerized actin (F-actin). Binding of Gag to F-actin may be involved in the assembly or budding of HIV-1...
  90. pmc Foamy virus Bet proteins function as novel inhibitors of the APOBEC3 family of innate antiretroviral defense factors
    Rebecca A Russell
    Jefferiss Trust Laboratories, Wright Fleming Institute, Imperial College, London, United Kingdom
    J Virol 79:8724-31. 2005
    ..While these data identify the foamy virus Bet protein as a functional ortholog of the HIV-1 Vif auxiliary protein, they also indicate that Vif and Bet block APOBEC3 protein function by distinct mechanisms...
  91. pmc Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells
    Elizabeth R Wright
    Division of Biology, California Institute of Technology, Pasadena, CA, USA
    EMBO J 26:2218-26. 2007
    ..This model suggests why the SP1 spacer is essential for assembly of the Gag lattice and how cleavage between SP1 and CA acts as a structural switch controlling maturation...
  92. ncbi HIV type 1 Gag and nucleocapsid proteins: cytoskeletal localization and effects on cell motility
    F J Ibarrondo
    Department of Pediatrics, UCLA School of Medicine, Los Angeles, California 90095, USA
    AIDS Res Hum Retroviruses 17:1489-500. 2001
    ..These data suggest that interactions between HIV-1 Gag and actin in infected cells enhance cell motility. Ultimately this enhanced motility of infected cells could promote the dissemination of virus into the brain and other tissues...
  93. pmc Analysis of total human immunodeficiency virus (HIV)-specific CD4(+) and CD8(+) T-cell responses: relationship to viral load in untreated HIV infection
    M R Betts
    Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9113, USA
    J Virol 75:11983-91. 2001
    ..These results suggest that overall frequencies of HIV-specific T cells are not the sole determinant of immune-mediated protection in HIV-infection...
  94. pmc Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses
    Virginie Sandrin
    Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, IFR128 Biosciences Lyon Gerland, Ecole Normal Supérieure de Lyon, France
    J Virol 78:7153-64. 2004
    ..Thus, intracellular colocalization, as well as interactions between Env and core proteins, may influence the recruitment of the glycoprotein onto viral particles and generate infectious pseudotyped viruses...
  95. pmc Retrovirus infection strongly enhances scrapie infectivity release in cell culture
    Pascal Leblanc
    LaboRetro Unité de virologie humaine INSERM U758, Ecole Normale Superieure de Lyon, Lyon Cedex, France
    EMBO J 25:2674-85. 2006
    ..Under these conditions, we found that PrPC, PrPSc and scrapie infectivity are recruited by both MuLV virions and exosomes. We propose that retroviruses can be important cofactors involved in the spread of the pathological prion agent...
  96. ncbi Tsg101 and Alix interact with murine leukemia virus Gag and cooperate with Nedd4 ubiquitin ligases during budding
    Carolina Segura-Morales
    IGMM CNRS, 1919 Route de Mende 34293, Montpellier Cedex 5, France
    J Biol Chem 280:27004-12. 2005
    ..1. Other Nedd4-dependent Gag proteins also contain binding sites for Tsg101 or Alix, suggesting that this could be a common feature of retroviruses...
  97. ncbi HIV-1 assembly and maturation
    A G Bukrinskaya
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, U S A
    Arch Virol 149:1067-82. 2004
    ..This brief review summarizes some recent findings on the final steps of the HIV-1 life cycle and touches upon some unanswered questions, particularly regarding the processes involved in virus maturation and infectivity...
  98. pmc Covalent modification of human immunodeficiency virus type 1 p6 by SUMO-1
    Cagan Gurer
    Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
    J Virol 79:910-7. 2005
    ..HIV-1 bearing the p6-K27R mutation was insensitive to SUMO-1 overexpression, suggesting that covalent attachment of SUMO-1 to p6 is detrimental to HIV-1 replication...
  99. ncbi HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane
    Dmitriy Mazurov
    National Cancer Institute, HIV Drug Resistance Program, Bld 535, Rm 110, NCI Frederick, Frederick, MD 21702 1201, USA
    Virology 346:194-204. 2006
    ..Our data suggest that once at the plasma membrane, HTLV-1 virion components associate with CD82-containing microdomains, which may facilitate the mobilization of nascent virions to sites of intercellular adhesion...
  100. pmc Direct measurement of Gag-Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy
    Daniel R Larson
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 162:1233-44. 2003
    ..These methods also have general applicability to in vivo studies of protein-protein and -membrane interactions involved in the formation of complex macromolecular structures...
  101. ncbi The critical role of proximal gag sequences in feline immunodeficiency virus genome encapsidation
    Iris Kemler
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Virology 327:111-20. 2004
    ..Focusing further on this proximal sequence, we found that a deletion of only 13 nt at the 5' end of gag impaired encapsidation of subgenomic vector and proviral RNAs...

Research Grants62

  1. Immunization Strategies for Autologous HIV Immunotherapy
    Louis D Falo; Fiscal Year: 2012
    ..The studies we propose include translational preclinical models designed as a direct prelude to human clinical trials. ..
  2. Monitoring single conformational events during HIV assembly
    WALTHER H MOTHES; Fiscal Year: 2013
    ..A detailed knowledge of the energy landscape and the kinetics of HIV assembly will aid in the identification of novel structural intermediates. This new information will be relevant for the rational design of antiviral therapies. ..
  3. Rational Design of antivrials targeted to HIV-1 capsid
    Asim K Debnath; Fiscal Year: 2013
    ..The studies described in this proposal may lead to the development of a new class of antiretroviral therapeutics targeting the HIV-1 capsid. ..
  4. Viral and Cellular Determinants of HIV-1 Assembly
    Paul W Spearman; Fiscal Year: 2013
    ....
  5. Dendritic Cell-Targeted HIV Vaccine Product
    Tibor Keler; Fiscal Year: 2011
    ....
  6. Membrane Coupling and Dynamic Reorganization of Gag in Viral Budding
    MATHIAS LOESCHE; Fiscal Year: 2013
    ..Its broader impact is to provide new techniques for the structural characterization of membrane proteins associated with lipid bilayers in their physiologically relevant, thermally disordered state. ..
  7. A New Method for Biomembrane Simulations
    Gregory A Voth; Fiscal Year: 2012
    ....
  8. High-throughput screening for HIV assembly and maturation inhibitors
    Wuyuan Lu; Fiscal Year: 2013
    ..Our long-term objective is to develop HIV assembly and maturation inhibitors as a novel class of therapeutic agents for the prevention of HIV-1 infection and the treatment of AIDS. ..
  9. NMR STUDIES OF HIV-1 PROTEINS
    MICHAEL FINLEY SUMMERS; Fiscal Year: 2013
    ..abstract_text> ..
  10. HIV Nucleocapsid Protein Nucleic Acid Chaperone Activity
    KARIN M MUSIER-FORSYTH; Fiscal Year: 2013
    ..The specific aims are: (1) To probe the NA chaperone activity of WT, mutant and precursor forms of HIV-1 NC, and (2) to probe HIV-1 Gag's chaperone activity in vitro and in vivo. ..
  11. Structural Basis for Long-Range Genetic Interactions in Retroviral CA Assembly
    Rebecca Craven; Fiscal Year: 2009
    ..A detailed understanding of this essential step of virus infection will allow development of better maturation inhibitors for use as antiretroviral drugs. ..
  12. MOUSE RETROVIRUS CO-OPTING OF IMMUNE CELL INTERACTIONS
    William Green; Fiscal Year: 2005
    ..abstract_text> ..
  13. TNT: Targeting and Triggering Adjuvancy of DNA Vaccines
    Michele Kutzler; Fiscal Year: 2005
    ..Taken together, this proposal aims to identify and characterize a unique TNT adjuvant combination for HIV-1 DNA vaccines resulting in enhanced antigen specific cell-mediated immune responses and improved HIV-1 DNA vaccine candidates. ..
  14. MOLECULAR BIOLOGY OF HIV1 GAG PROTEINS
    Heinrich Gottlinger; Fiscal Year: 2003
    ..An understanding of the molecular requirements for the association of these viral and cellular proteins with HIV-1 virions could provide specific targets for anti-viral therapy. ..
  15. GENETICS OF PRIMATE D TYPE RETROVIRUSES
    Eric Hunter; Fiscal Year: 1999
    ..The carboxy-terminal amino acid sequence of the five major gag gene products of this virus will be determined...
  16. GENETICS OF PRIMATE D TYPE RETROVIRUSES
    Eric Hunter; Fiscal Year: 2001
    ..Screen for inhibitors of the proteinase using a combinatorial peptide library; 2d. Crystallize and determine the structure of the different forms of the proteinase. ..
  17. HIV-1 Gag structure as a therapeutic target
    Mamuka Kvaratskhelia; Fiscal Year: 2007
    ..Potential outcome of these studies include development of a new method that could be exploited for screening of larger chemical libraries to discover novel potent anti-assembly inhibitors. [unreadable] [unreadable] [unreadable]..
  18. MOLECULAR ANALYSIS OF HIV REPLICATION
    Maxine Linial; Fiscal Year: 1992
    ....
  19. NONREPLICATING MODIFIED VIRUS LIKE PARTICLES (VLPS) VAC
    Polly Roy; Fiscal Year: 1999
    ..Once optimal production method for VLPs is established, VLPs presenting all clades of HIV-1 Env and Gag antigens will be generated using baculovirus multigene expression system. ..
  20. KIF4 motor protein, regulates trafficking and stability of HIV-1 Gag
    Nathaniel W Martinez; Fiscal Year: 2010
    ..Gag trafficking and virus particle assembly remains an unexplored field for targeting &development of novel pharmaceutical inhibitors needed to treat HIV infections. ..
  21. ANALYSIS OF RETROVIRUS ASSEMBLY BY IN VITRO MUTAGENESIS
    John Wills; Fiscal Year: 1990
    ..The long-range goal of the work proposed here is to elucidate the roles of the gag gene products in the assembly of Rous sarcoma virus (RSV), the prototype retrovirus...
  22. A computer model for the membrane binding of HIV-1 Gag
    Diana Murray; Fiscal Year: 2003
    ..abstract_text> ..
  23. HUMAN FOAMY VIRUS STRUCTURAL PROTEINS
    Paul Goepfert; Fiscal Year: 2000
    ..A better understanding of the human member of this remarkable but relatively neglected genus of retroviruses will enhance our knowledge of both viral and cellular mechanisms. ..
  24. HOST CELL PROTEINS IN RETROVIRAL ASSEMBLY & TRANSPORT
    Rachel LaCasse; Fiscal Year: 2002
    ..abstract_text> ..
  25. Immunogenicity of HSV Amplicons in Rhesus Macaques
    Luis Giavedoni; Fiscal Year: 2004
    ..In addition, the successful completion of these studies will have a significant impact on the continued development of HSV amplicons as gene and cancer therapy vectors. ..
  26. BIOPHYSICAL STUDIES OF HIV ASSEMBLY AND MATURATION
    Peter E Prevelige; Fiscal Year: 2010
    ..abstract_text> ..
  27. The Role of Matrix Cofactors in SIVcpz Replication
    Frederic Bibollet Ruche; Fiscal Year: 2009
    ..The knowledge gained from this project and the SIVcpz-chimpanzee model can be used to expand our understanding of HIV-1 MA protein interactions and could lead to new approaches for treatment of HIV-1 in patients. ..
  28. Intracellular Localization of HIV-1 RNAs by Rev&Matrix
    Michael Green; Fiscal Year: 2005
    ..Finally, our results suggest a novel method for inhibiting the activity of any mRNA. This "mRNA nuclear entrapment" strategy will be developed and tested using HIV-1 replication as a model system. ..
  29. EIAV Control by High Avidity Rev-Specific CTL Clones
    ROBERT MEALEY; Fiscal Year: 2005
    ..The information obtained from the proposed studies should have implications for HIV-1 immunotherapy and vaccine design, where experiments of this type may be more difficult. ..
  30. HIV-1 Suppression by MHC class I restricted CD8 T Cells
    Paul Goepfert; Fiscal Year: 2008
    ..Our findings will have important implications fro HIV immunopathogenesis and vaccine design by supplying an HIV-1 specific CD8 T cell phenotype that results in efficient HIV-1 control. [unreadable] [unreadable] [unreadable]..
  31. Structural Biology of HIV Assembly, Budding, and Entry
    Wesley Sundquist; Fiscal Year: 2006
    ..In summary, our Program will provide significant insights into how the HIV virion functions as a machine that can enter and exit cells, and how these processes can be inhibited. ..
  32. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2009
    ..Multicolor imaging with site-specific fluorescein derivatives (FLASH and ReAsH) will be used to determine the order in which Gag assembly sites are formed and maintained in the cell. ..
  33. AN EVOLUTIONARY LINK BETWEEN TELOMERES AND TRANSPOSONS
    MARY LOU PARDUE; Fiscal Year: 2008
    ..We have discovered that HeT-A and TART expression can be regulated by several external agents. We describe experiments to better define the set of agents capable of this regulation and to explore possible mechanisms. ..
  34. Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cells
    JAISRI LINGAPPA; Fiscal Year: 2008
    ..abstract_text> ..
  35. MVA/HIV-48: Potential Dual Vaccine for HIV and Smallpox
    Rama Rao Amara; Fiscal Year: 2003
    ..The project will be a collaborative undertaking between researchers at the CDC, the NIAID, and the NIAID HIV Vaccine Trial Network (HVTN ). The focus of this application is the quantification and phenotyping of responding T cells. ..
  36. STRUCTURE/FUNCTION OF HIV1 CAPSID/CYCLOPHILIN COMPLEX
    Wesley Sundquist; Fiscal Year: 2001
    ..Finally, genetic analysis of mutants of CA in an HIV provirus will be used to test models that arise from the CA and CA-cyclophilin structures. ..
  37. ALABAMA/ZAMBIA HIV VACCINE CLINICAL TRIALS UNIT (HVTU)
    Paul Goepfert; Fiscal Year: 2006
    ..Recently, the Alabama clinic enrolled 125 higher-risk gay men in <5 months in the first US phase III trial. Plans for utilizing these cohorts and others are described. ..
  38. Poly-functional analyses of vaccine-induced T cell responses
    Guido Ferrari; Fiscal Year: 2010
    ..Overall, these findings will shed new insights on the importance and fate of vaccine-induced T cell responses in controlling HIV-infection. ..
  39. AN EVOLUTIONARY LINK BETWEEN TELOMERES AND TRANSPOSONS
    MARY LOU PARDUE; Fiscal Year: 2006
    ..abstract_text> ..
  40. EIAV Control by DNA Vaccine-Induced CTL
    ROBERT MEALEY; Fiscal Year: 2006
    ..The information obtained from the proposed studies should have implications for HIV-1 vaccine design, where experiments of this type may be more difficult. [unreadable] [unreadable]..
  41. Glial cell differentiation and glioma formation
    MARILYN RESH; Fiscal Year: 2006
    ..The activity of the above proteins in human glioma tumor samples will be determined. ..
  42. Immune Control of HIV in Children
    Margaret Feeney; Fiscal Year: 2006
    ..This research will be conducted under the guidance of leading investigators in the field of HIV immunopathogenesis in adults. ..
  43. PROTEINS OF TELOMERE RETROTRANSPOSABLE ELEMENTS
    MARY PARDUE; Fiscal Year: 2001
    ....
  44. Role of Lysyl-tRNA Synthetase in HIV-1 Replication
    Lawrence Kleiman; Fiscal Year: 2006
    ..Both biochemical purification methods and a genetic strategy for identifying cDNAs coding for LysRS proteases will be used to identify cellular proteases that can cleave LysRS. ..
  45. Cell-free VEE assembly system and alphavirus drug screen
    JAISRI LINGAPPA; Fiscal Year: 2006
    ..The cell-free VEE assembly system offer a safe and low cost approach to understanding the mechanism of VEE capsid formation and to screen for agents active against alphaviruses that have potential as bioterrorism agents. ..
  46. Mucosal and Peripheral NK-T Cells in HIV Infection
    Otto Yang; Fiscal Year: 2007
    ..Exploring NK-T cells in HIV-1 infection could there have a high impact on the development of new strategies in prevention and treatment. [unreadable] [unreadable]..
  47. CD8+ T cell cytokine production in HIV-1 infection
    Paul Goepfert; Fiscal Year: 2005
    ..An HIV-1 vaccine would also be enhanced by identifying CD8+ T cell epitopes that portend a favorable prognosis and are difficult for the virus to mutate (specific aims 2 and 3). ..
  48. TNFSF APC activators for HIV Vaccines
    RICHARD KORNBLUTH; Fiscal Year: 2003
    ..Upon the completion of these feasibility studies, it will be clear if these concepts should be advanced into more extensive vaccine studies both for HIV and other pathogens. ..
  49. The impact of early antiretroviral therapy on HIV persistence and inflammation
    STEVEN GRANT DEEKS; Fiscal Year: 2010
    ..Our work may also provide important insights in the role of chronic inflammation in driving viral persistence, and hence may lead to the development of novel interventions. ..
  50. HIV Vaccine Development
    David Montefiori; Fiscal Year: 2003
    ....
  51. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    Stephen Goff; Fiscal Year: 2003
    ..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
  52. FOAMY VIRUS INFECTION IN VITRO AND IN VIVO
    Maxine Linial; Fiscal Year: 2005
    ..These experiments will be will be important for the assessment of the utility of foamy viruses as vectors for gene therapy. ..
  53. Latent virus and HIV-1 specific immunity in LTNPs
    Joel Blankson; Fiscal Year: 2006
    ..Siliciano, and the supportive environment of the Johns Hopkins University will provide Dr. Blankson with the skills he needs to develop into an independent clinician and basic scientist researcher in HIV-1 pathogenesis. ..
  54. A hybrid 12T Q-FTMS for the investigation of nucleic acids non-cov./cov.adducts
    Daniele Fabris; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  55. Implications of HIV in Low HCV Clearance in Chinese IDUs
    Xiao Fang Yu; Fiscal Year: 2007
    ..The results of these studies should provide further insight into the mechanism of HCV clearance and the development of immunity which are critical for the development of effective vaccine and new treatment strategies. ..
  56. MOLECULAR ANALYSIS OF A HIGHLY PATHOGENIC FIV
    John Elder; Fiscal Year: 2008
    ..abstract_text> ..
  57. Dissecting HIV integration vs production in CD4+ T cells
    UNA O DOHERTY; Fiscal Year: 2008
    ..We will then apply what we learn from our in vitro studies to define more precisely the phenotype(s) of the CD4+ resting T cells that are latently infected in patients. ..
  58. Effect of Schistosoma Co-infection on HIV Immune Responses
    Huyen Cao; Fiscal Year: 2007
    ..mansoni infection dysregulates cellular immune responses by enhancing the activity of IL-10-producing T cells. S. mansoni co-infection will therefore be examined in the context of being an experimental immune modulator. ..
  59. Cell immune responses in SIV-infected sooty mangabeys
    Amitinder Kaur; Fiscal Year: 2004
    ..abstract_text> ..
  60. VACCINE-ELICITED SIV-SPECIFIC CTL
    WILLIAM CHARINI; Fiscal Year: 2004
    ..In addition, a committee of distinguished scientists including two AIDS researchers and an immunologist, will oversee Dr. Charini's progress. ..
  61. Immunologic Control of Drug-resistant HIV-1
    Steven Deeks; Fiscal Year: 2005
    ..Finally, since we will estimate the relative in vivo thresholds for HIV-mediated immunogenicity and pathogenicity, these studies may also have implications for vaccine development. ..