non histone chromosomal proteins

Summary

Summary: Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.

Top Publications

  1. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
  2. ncbi Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
    X Nan
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 393:386-9. 1998
  3. ncbi Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Mol Cell 5:243-54. 2000
  4. ncbi A molecular determinant for the establishment of sister chromatid cohesion
    Elcin Unal
    Howard Hughes Medical Institute and Department of Embryology, Carnegie Institution, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Science 321:566-9. 2008
  5. ncbi Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion
    Tom Rolef Ben-Shahar
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln sInn Fields, London WC2A 3PX, UK
    Science 321:563-6. 2008
  6. pmc Identification of cis-acting sites for condensin loading onto budding yeast chromosomes
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    Genes Dev 22:2215-27. 2008
  7. ncbi Genome regulation by polycomb and trithorax proteins
    Bernd Schuettengruber
    Institute of Human Genetics, CNRS, 141, rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Cell 128:735-45. 2007
  8. ncbi Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer
    I Versteege
    Laboratoire de Pathologie Moléculaire des Cancers, Section de Recherche, Institut Curie, Paris, France
    Nature 394:203-6. 1998
  9. ncbi G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis
    Nirit Feldman
    Department of Experimental Medicine, Hebrew University Medical School, Ein Kerem, Jerusalem 91120, Israel
    Nat Cell Biol 8:188-94. 2006
  10. pmc A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae
    V Guacci
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Cell 91:47-57. 1997

Research Grants

Detail Information

Publications281 found, 100 shown here

  1. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
    ..Proteins homologous to Scc1p exist in a variety of eukaryotic organisms including humans. A common cohesion apparatus might be used by all eukaryotic cells during both mitosis and meiosis...
  2. ncbi Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
    X Nan
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 393:386-9. 1998
    ..The data suggest that two global mechanisms of gene regulation, DNA methylation and histone deacetylation, can be linked by MeCP2...
  3. ncbi Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Mol Cell 5:243-54. 2000
    ..Our data suggest that a major role for the Scc2p/Scc4p complex is to facilitate the loading of cohesin complexes onto chromosomes...
  4. ncbi A molecular determinant for the establishment of sister chromatid cohesion
    Elcin Unal
    Howard Hughes Medical Institute and Department of Embryology, Carnegie Institution, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Science 321:566-9. 2008
    ..Smc3 head acetylation and its cell cycle regulation provide important insights into the biology and mechanism of cohesion establishment...
  5. ncbi Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion
    Tom Rolef Ben-Shahar
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln sInn Fields, London WC2A 3PX, UK
    Science 321:563-6. 2008
    ..Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent...
  6. pmc Identification of cis-acting sites for condensin loading onto budding yeast chromosomes
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    Genes Dev 22:2215-27. 2008
    ..This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis...
  7. ncbi Genome regulation by polycomb and trithorax proteins
    Bernd Schuettengruber
    Institute of Human Genetics, CNRS, 141, rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Cell 128:735-45. 2007
    ..Recent work suggests that PcG proteins regulate the nuclear organization of their target genes and that PcG-mediated gene silencing involves noncoding RNAs and the RNAi machinery...
  8. ncbi Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer
    I Versteege
    Laboratoire de Pathologie Moléculaire des Cancers, Section de Recherche, Institut Curie, Paris, France
    Nature 394:203-6. 1998
    ..The observation of bi-allelic alterations of hSNF5/INI1 in MRTs suggests that loss-of-function mutations of hSNF5/INI1 contribute to oncogenesis...
  9. ncbi G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis
    Nirit Feldman
    Department of Experimental Medicine, Hebrew University Medical School, Ein Kerem, Jerusalem 91120, Israel
    Nat Cell Biol 8:188-94. 2006
    ..Genetic studies show that these epigenetic changes actually have an important role in the inhibition of Oct-3/4 re-expression, thereby preventing reprogramming...
  10. pmc A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae
    V Guacci
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Cell 91:47-57. 1997
    ..These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link...
  11. ncbi Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast
    Jinglan Zhang
    Center for Molecular Discovery, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell 31:143-51. 2008
    ..Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion...
  12. ncbi Shugoshin collaborates with protein phosphatase 2A to protect cohesin
    Tomoya S Kitajima
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Nature 441:46-52. 2006
    ..Thus, we have revealed a conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis...
  13. ncbi At the heart of the chromosome: SMC proteins in action
    Tatsuya Hirano
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Mol Cell Biol 7:311-22. 2006
    ..An emerging idea is that SMC proteins use a diverse array of intramolecular and intermolecular protein-protein interactions to actively fold, tether and manipulate DNA strands...
  14. pmc Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP
    Daniel R Foltz
    Ludwig Institute for Cancer Research, La Jolla, CA 92093 0670, USA
    Cell 137:472-84. 2009
    ..We propose HJURP to be a cell-cycle-regulated CENP-A-specific histone chaperone required for centromeric chromatin assembly...
  15. ncbi Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits
    M L Phelan
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    Mol Cell 3:247-53. 1999
    ..The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex...
  16. pmc Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells
    Yamini Dalal
    Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS Biol 5:e218. 2007
    ..This demonstration of stable half-nucleosomes in vivo provides a possible basis for the instability of centromeric nucleosomes that are deposited in euchromatic regions, which might help maintain centromere identity...
  17. ncbi Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    Thomas A Volpe
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Science 297:1833-7. 2002
    ..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
  18. ncbi Postreplicative recruitment of cohesin to double-strand breaks is required for DNA repair
    Lena Ström
    Department of Cell and Molecular Biology, Karolinska Institute, Berzelius vag 35, 171 77 Stockholm, Sweden
    Mol Cell 16:1003-15. 2004
    ..Evidence is also provided that DSB induction elicits establishment of sister chromatid cohesion in G2, implicating that damage-recruited cohesin facilitates DNA repair by tethering chromatids...
  19. ncbi HP1 and the dynamics of heterochromatin maintenance
    Christèle Maison
    Unité Mixte de Reserche 218, Centre National de la Recherche Scientifique Institut Curie Section de Recherche, 26 Rue d Ulm, 75231 Paris Cedex 05, France
    Nat Rev Mol Cell Biol 5:296-304. 2004
  20. ncbi Recombination regulation by transcription-induced cohesin dissociation in rDNA repeats
    Takehiko Kobayashi
    National Institute for Basic Biology, SOKENDAI, School of Life Science, 38 Nishigonaka, Myodaijicho, Okazaki, 444 8585 Japan
    Science 309:1581-4. 2005
    ..This transcription is regulated by the silencing gene, SIR2, and by copy number. Transcription-induced cohesin dissociation may be a general mechanism of recombination regulation...
  21. pmc Purification and biochemical heterogeneity of the mammalian SWI-SNF complex
    W Wang
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, CA 94305 5428, USA
    EMBO J 15:5370-82. 1996
    ..Certain cell lines completely lack BRG1 and hbrm, indicating that they are not essential for cell viability and that the mammalian SWI-SNF complex may be tailored to the needs of a differentiated cell type...
  22. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
    ..This model may also explain the stable inheritance of the heterochromatic state...
  23. ncbi Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein
    S B Seo
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Cell 104:119-30. 2001
    ..We propose that histone masking by INHAT plays a regulatory role in chromatin modification and serves as a novel mechanism of transcriptional regulation...
  24. ncbi Molecular architecture of SMC proteins and the yeast cohesin complex
    Christian H Haering
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 9:773-88. 2002
    ..The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication...
  25. pmc The CENP-S complex is essential for the stable assembly of outer kinetochore structure
    Miho Amano
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 186:173-82. 2009
    ..In addition, we found that intrakinetochore distance was increased in CENP-S- and CENP-X-deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore...
  26. ncbi Establishment and maintenance of a heterochromatin domain
    Ira M Hall
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 297:2232-7. 2002
    ..This work defines sequential requirements for the initiation and propagation of regional heterochromatic domains...
  27. ncbi Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity
    Benjamin D Rowland
    University of Oxford, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
    Mol Cell 33:763-74. 2009
    ..According to this model, Eco1 promotes sister DNA entrapment partly by relieving an antiestablishment activity associated with Scc3, Pds5, and Rad61...
  28. ncbi Sororin is required for stable binding of cohesin to chromatin and for sister chromatid cohesion in interphase
    Julia Schmitz
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 17:630-6. 2007
    ..Our data indicate that sororin interacts with chromatin-bound cohesin and functions during the establishment or maintenance of cohesion in S or G2 phase, respectively...
  29. pmc CTCF physically links cohesin to chromatin
    Eric D Rubio
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle WA 98195, USA
    Proc Natl Acad Sci U S A 105:8309-14. 2008
    ..These results have implications for the functional role of cohesin subunits in the pathogenesis of Cornelia de Lange syndrome and Roberts syndromes...
  30. ncbi An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 93:1067-76. 1998
    ..We propose that the APC promotes sister separation not by destroying cohesins but instead by liberating the "sister-separating" Esp1 protein from its inhibitor Pds1p...
  31. pmc A novel nuclear structure containing the survival of motor neurons protein
    Q Liu
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    EMBO J 15:3555-65. 1996
    ..These structures are similar in number (2-6) and size (0.1-1.0 micron) to coiled bodies, and frequently are found near or associated with coiled bodies. We term these prominent nuclear structures gems, for Gemini of coiled bodies...
  32. ncbi Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription
    P L Jones
    Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland 20892 5431, USA
    Nat Genet 19:187-91. 1998
    ..These results establish a direct causal relationship between DNA methylation-dependent transcriptional silencing and the modification of chromatin...
  33. ncbi Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4
    A Verreault
    Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 87:95-104. 1996
    ..Our data suggest that CAC is a key intermediate of the de novo nucleosome assembly pathway and that the p48 subunit participates in other aspects of histone metabolism...
  34. ncbi Wapl controls the dynamic association of cohesin with chromatin
    Stephanie Kueng
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Cell 127:955-67. 2006
    ..Wapl depletion also increases the residence time of cohesin on chromatin in interphase. Our data indicate that Wapl is required to unlock cohesin from a particular state in which it is stably bound to chromatin...
  35. ncbi Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region
    Y Blat
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 98:249-59. 1999
    ..The methodology described can be used to analyze the distribution of any DNA-binding protein and, via microchips, along entire genomes...
  36. ncbi Chromosomal passengers: conducting cell division
    Sandrine Ruchaud
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, King s Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK
    Nat Rev Mol Cell Biol 8:798-812. 2007
    ....
  37. pmc Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers
    Thomas D Barber
    The Ludwig Center and Howard Hughes Medical Institute at the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 105:3443-8. 2008
    ..These results suggest that defective sister chromatid cohesion as a result of somatic mutations may represent a major cause of chromosome instability in human cancers...
  38. pmc Budding yeast chromosome structure and dynamics during mitosis
    C G Pearson
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 152:1255-66. 2001
    ..These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms...
  39. ncbi Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin
    K Shibahara
    Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 96:575-85. 1999
    ..We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states...
  40. ncbi The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides
    Edward A Bayer
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    Annu Rev Microbiol 58:521-54. 2004
    ....
  41. pmc Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
    Claire Ditchfield
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK
    J Cell Biol 161:267-80. 2003
    ..Together, these results suggest that by targeting checkpoint proteins to kinetochores, Aurora B couples chromosome alignment with anaphase onset...
  42. ncbi Identification of cohesin association sites at centromeres and along chromosome arms
    T Tanaka
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 98:847-58. 1999
    ..Finally, cohesion associated with "minimal" centromeres is insufficient to resist the splitting force exerted by microtubules and appears to be reinforced by cohesion provided by their flanking DNA sequences...
  43. ncbi Evidence for the existence of an HP1-mediated subcode within the histone code
    Gwen Lomberk
    Gastroenterology Research Unit, Department of Medicine, and Mayo Clinic Cancer Center, Rochester, MN 55605, USA
    Nat Cell Biol 8:407-15. 2006
    ....
  44. pmc Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1beta and SMC3
    Maureen Eijpe
    Molecular Genetics Group, Botanical Center, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
    J Cell Biol 160:657-70. 2003
    ..We propose that REC8 then provides cohesion. RAD51 and/or DMC1 coimmunoprecipitates with REC8, suggesting that REC8 may also provide a basis for assembly of recombination complexes...
  45. ncbi Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae
    M L Mayer
    Graduate Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Cell 7:959-70. 2001
    ....
  46. ncbi Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1
    Q Ye
    Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Biol Chem 271:14653-6. 1996
    ..LBR can interact with chromodomain proteins that are highly conserved in eukaryotic species and may function in the attachment of heterochromatin to the inner nuclear membrane in cells...
  47. ncbi A genome-wide screen identifies genes required for centromeric cohesion
    Adele L Marston
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames Street, Cambridge, MA 02139, USA
    Science 303:1367-70. 2004
    ..The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identifies three factors important for the stepwise loss of sister chromatid cohesion...
  48. pmc Pericentric chromatin is organized into an intramolecular loop in mitosis
    Elaine Yeh
    Department of Biology, 623 Fordham Hall CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Curr Biol 18:81-90. 2008
    ..Cohesin is concentrated at the centromere region of the chromosome despite the fact that sister centromeres can be separated by 800 nm in vivo. The function of cohesin at sites of separated DNA is unknown...
  49. pmc Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion
    Robert V Skibbens
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Genetics 166:33-42. 2004
    ..Our findings document a novel role for Chl1p in sister-chromatid cohesion and provide new insights into the possible mechanisms through which DNA helicases may contribute to cancer progression when mutated...
  50. ncbi Dual inhibition of sister chromatid separation at metaphase
    O Stemmann
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 107:715-26. 2001
    ..We propose that separase activation at the metaphase-anaphase transition requires the removal of both securin and an inhibitory phosphate...
  51. ncbi Live-cell imaging reveals a stable cohesin-chromatin interaction after but not before DNA replication
    Daniel Gerlich
    Gene Expression Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Curr Biol 16:1571-8. 2006
    ....
  52. ncbi A family of zinc-finger proteins is required for chromosome-specific pairing and synapsis during meiosis in C. elegans
    Carolyn M Phillips
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Dev Cell 11:817-29. 2006
    ..To gain insight into the evolution of these components, we characterized homologs in C. briggsae and C. remanei, which revealed changes in copy number of this gene family within the nematode lineage...
  53. ncbi Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis
    Hideaki Tagami
    Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Cell 116:51-61. 2004
    ..This finding provides new insights into possible mechanisms for maintenance of epigenetic information after chromatin duplication...
  54. ncbi Heterochromatin revisited
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Genet 8:35-46. 2007
    ....
  55. pmc Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2
    Silke Hauf
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e69. 2005
    ..The similarity of the phenotype obtained after expression of nonphosphorylatable SA2 in human cells to that seen after the depletion of Plk1 suggests that SA2 is the critical target of Plk1 in the cohesin dissociation pathway...
  56. ncbi The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes
    Vahan B Indjeian
    Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 307:130-3. 2005
    ....
  57. ncbi A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
    T Hirano
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Cell 79:449-58. 1994
    ..These motifs are highly conserved in a protein family, members of which have been identified recently in both prokaryotes and eukaryotes...
  58. ncbi Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells
    E Minc
    Departement de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universite Paris 7, Paris, France
    Chromosoma 108:220-34. 1999
    ..Thus, subtle differences in primary sequence and in posttranslational modifications may promote their targeting at different chromatin sites, generating pleiotropic effects...
  59. ncbi MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin
    X Nan
    Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom
    Cell 88:471-81. 1997
    ..These properties, together with the abundance of MeCP2 and the high frequency of its 2 bp binding site, suggest a role as a global transcriptional repressor in vertebrate genomes...
  60. pmc Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin
    Lucia Piacentini
    Istituto Pasteur, Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Roma, Italy
    J Cell Biol 161:707-14. 2003
    ..These data significantly broaden the current views of the roles of HP1 in vivo by demonstrating that this protein has multifunctional roles...
  61. ncbi Cohesin mediates transcriptional insulation by CCCTC-binding factor
    Kerstin S Wendt
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Nature 451:796-801. 2008
    ..We propose that cohesin functions as a transcriptional insulator, and speculate that subtle deficiencies in this function contribute to 'cohesinopathies' such as Cornelia de Lange syndrome...
  62. ncbi Centrosome-dependent exit of cytokinesis in animal cells
    M Piel
    Institut Curie, Section Recherche, UMR 144 du CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France
    Science 291:1550-3. 2001
    ....
  63. ncbi The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci
    Raymond A Poot
    Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
    Nat Cell Biol 6:1236-44. 2004
    ..We propose that the WSTF-ISWI complex has a role in the maintenance of chromatin structures during DNA replication...
  64. pmc Heterochromatin protein 1 is recruited to various types of DNA damage
    Martijn S Luijsterburg
    Swammerdam Institute for Life Sciences, Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1012 WX Amsterdam, Netherlands
    J Cell Biol 185:577-86. 2009
    ..Our results show that HP1 proteins are important for DNA repair and may function to reorganize chromatin in response to damage...
  65. ncbi Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype
    A G Uren
    The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, 3050, Victoria, Australia
    Curr Biol 10:1319-28. 2000
    ..To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice...
  66. pmc A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction
    P Pasierbek
    Department of Cytology and Genetics, Institute of Botany, University of Vienna, A 1030 Vienna, Austria
    Genes Dev 15:1349-60. 2001
    ..The loss of yeast Rec8p from chromosome arms at meiosis I and centromeres at meiosis II coordinates the disjunction of homologs and sister chromatids at the two meiotic divisions...
  67. pmc Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase
    Rita Gandhi
    Cold Spring Harbor Laboratory, One Bungtown Road, P O Box 100, Cold Spring Harbor, New York 11724, USA
    Curr Biol 16:2406-17. 2006
    ..This process is known as sister-chromatid resolution. Although two mitotic kinases have been implicated in this process, it remains unknown exactly how the cohesin-mediated linkage is destabilized at a mechanistic level...
  68. ncbi ATP hydrolysis is required for cohesin's association with chromosomes
    Prakash Arumugam
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 13:1941-53. 2003
    ..These Smc "heads" are connected by Scc1, creating a tripartite proteinaceous ring...
  69. ncbi Securin is required for chromosomal stability in human cells
    P V Jallepalli
    The Johns Hopkins Oncology Center, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cell 105:445-57. 2001
    ..These results illuminate the function of mammalian securin and show that it is essential for the maintenance of euploidy...
  70. ncbi A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage
    Elies C Woudstra
    Mechanisms of Gene Transcription Laboratory, Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 415:929-33. 2002
    ....
  71. ncbi Molecular biology. Chromatin higher order folding--wrapping up transcription
    Peter J Horn
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Science 297:1824-7. 2002
    ..We also discuss evidence suggesting that adenosine-5'-triphosphate-dependent chromatin-remodeling enzymes and histone-modifying enzymes may regulate transcription by controlling the extent and dynamics of chromatin higher order folding...
  72. ncbi Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Cell 126:1049-64. 2006
    ....
  73. pmc Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae
    G A Hartzog
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 12:357-69. 1998
    ..1998), provide strong evidence that these factors are important for transcription elongation in vivo...
  74. pmc Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin
    Abarna Thiru
    Department of Biochemistry, Cambridge Centre for Molecular Recognition, University of Cambridge, UK
    EMBO J 23:489-99. 2004
    ..This finding implies a further complexity to the histone code for regulation of chromatin structure and suggests how binding of HP1 family proteins may lead to its condensation...
  75. pmc Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin
    Alison L Pidoux
    Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, 6 34 Swann Building, Edinburgh EH93JR, Scotland, UK
    Mol Cell 33:299-311. 2009
    ....
  76. pmc Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis
    Reiko Honda
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 14:3325-41. 2003
    ..A nonphosphorylatable mutant (TSS893-895AAA) was a poor activator of Aurora B, demonstrating that INCENP phosphorylation is important for kinase activation...
  77. ncbi Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis
    Huiling Xu
    Divisions of Radiation Oncology and Research, Peter MacCallum Cancer Centre, Melbourne, Victoria 8006, Australia
    Dev Cell 8:949-61. 2005
    ..Unexpectedly, Rec8 null mice are born in sub-Mendelian frequencies and fail to thrive. These findings illuminate hitherto unknown REC8 functions in chromosome dynamics during mammalian meiosis and possibly in somatic development...
  78. ncbi Cohesin: its roles and mechanisms
    Kim Nasmyth
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Annu Rev Genet 43:525-58. 2009
    ..Recent evidence suggests that cohesin acts as a novel topological device that traps chromosomal DNA within a large tripartite ring formed by its core subunits...
  79. ncbi CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment
    K W Wood
    Laboratory of Cell Biology, Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla 92093 0660, USA
    Cell 91:357-66. 1997
    ..We further demonstrate that CENP-E powers movement toward microtubule plus ends in vitro. These findings support a model in which CENP-E functions in congression to tether kinetochores to dynamic microtubule plus ends...
  80. pmc Propagation of centromeric chromatin requires exit from mitosis
    Lars E T Jansen
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 176:795-805. 2007
    ....
  81. pmc High-resolution mapping reveals links of HP1 with active and inactive chromatin components
    Elzo de Wit
    Department of Molecular Biology, Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS Genet 3:e38. 2007
    ..These results demonstrate that HP1-chromatin is transcriptionally active and has extensive links with several other chromatin components...
  82. pmc In vivo HP1 targeting causes large-scale chromatin condensation and enhanced histone lysine methylation
    Pernette J Verschure
    Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, P O Box 94062, 1090 GB Amsterdam, The Netherlands
    Mol Cell Biol 25:4552-64. 2005
    ..Our findings indicate that HP1alpha and HP1beta targeting is sufficient to induce heterochromatin formation...
  83. ncbi Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1)
    N Koike
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Japan
    FEBS Lett 467:17-21. 2000
    ..These results suggest that Pim-1 affects the structure or silencing of chromatin by phosphorylating HP1...
  84. pmc Two human orthologues of Eco1/Ctf7 acetyltransferases are both required for proper sister-chromatid cohesion
    Fajian Hou
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Mol Biol Cell 16:3908-18. 2005
    ..We propose that EFO1 and EFO2 are targeted to different chromosome structures to help establish or maintain sister-chromatid cohesion...
  85. ncbi The centromere paradox: stable inheritance with rapidly evolving DNA
    S Henikoff
    Howard Hughes Medical Institute Research Laboratories, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 293:1098-102. 2001
    ..Incompatibilities between rapidly evolving centromeric components may be responsible for both the organization of centromeric regions and the reproductive isolation of emerging species...
  86. pmc Regulation of the Drosophila Enhancer of split and invected-engrailed gene complexes by sister chromatid cohesion proteins
    Cheri A Schaaf
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, Saint Louis, Missouri, USA
    PLoS ONE 4:e6202. 2009
    ..We propose that cohesin helps restrain transcription of these gene complexes, and that deregulation of similarly cohesin-hypersensitive genes may underlie developmental deficits in Cornelia de Lange syndrome...
  87. pmc A handcuff model for the cohesin complex
    Nenggang Zhang
    Department of Pediatric Hematology Oncology, Texas Children s Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA
    J Cell Biol 183:1019-31. 2008
    ..These data support a two-ring handcuff model for the cohesin complex, which is flexible enough to establish and maintain sister chromatid cohesion as well as ensure the fidelity of chromosome segregation in higher eukaryotes...
  88. pmc Cohesin promotes the repair of ionizing radiation-induced DNA double-strand breaks in replicated chromatin
    Christina Bauerschmidt
    Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford OX3 7DQ, UK
    Nucleic Acids Res 38:477-87. 2010
    ..Our results suggest that the cohesin complex contributes to cell survival by promoting the repair of radiation-induced DNA double-strand breaks in G2-phase cells in an ATM-dependent pathway...
  89. ncbi Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5
    G V Kalpana
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, NY 10032
    Science 266:2002-6. 1994
    ..The gene, termed INI1 (for integrase interactor 1), may encode a nuclear factor that promotes integration and targets incoming viral DNA to active genes...
  90. ncbi Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners
    Alexander Schleiffer
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Mol Cell 11:571-5. 2003
    ..Scc1 interacts with SMC proteins through N- and C-terminal domains to form a ring-like structure. Since these are the only domains conserved among kleisins, we suggest that ring formation with SMC proteins may define this family...
  91. pmc Painting of fourth in genus Drosophila suggests autosome-specific gene regulation
    Jan Larsson
    Umeå Centrum för Molekylär Patogenes, Umea University, SE 901 87 Umea, Sweden
    Proc Natl Acad Sci U S A 101:9728-33. 2004
    ..The chromosome specificity is determined at the species level and not by the amino acid sequence. We argue that POF is involved in a chromosome-specific regulatory function...
  92. pmc CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
    H Liao
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 130:507-18. 1995
    ..The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events...
  93. pmc Condensin regulates the stiffness of vertebrate centromeres
    Susana A Ribeiro
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Mol Biol Cell 20:2371-80. 2009
    ..Loss of stiffness caused by condensin-depletion produces abnormal uncoordinated sister kinetochore movements, leads to an increase in Mad2(+) kinetochores near the metaphase plate and delays anaphase onset...
  94. ncbi The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication
    P D Kaufman
    Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 81:1105-14. 1995
    ..p150 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in human cell extracts, suggesting that such complexes are intermediates between histone synthesis and assembly onto replicating DNA...
  95. ncbi Polycomb silencing mechanisms and the management of genomic programmes
    Yuri B Schwartz
    Department of Molecular Biology and Biochemistry, Rutgers University, Nelson Laboratories, 604 Allison Road, Piscataway, New Jersey 08854, USA
    Nat Rev Genet 8:9-22. 2007
    ..These epigenetic states must be reprogrammed when cells become committed to differentiation...
  96. ncbi SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family
    A V Strunnikov
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210
    Genes Dev 9:587-99. 1995
    ....
  97. ncbi The meiotic protein SWI1 is required for axial element formation and recombination initiation in Arabidopsis
    Raphael Mercier
    School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, UK
    Development 130:3309-18. 2003
    ..The possible inter-relationship between these processes and the function of SWI1 are discussed...
  98. pmc Painting of fourth and chromosome-wide regulation of the 4th chromosome in Drosophila melanogaster
    Anna Mia Johansson
    UCMP, Umea University, Umea, Sweden
    EMBO J 26:2307-16. 2007
    ..We propose a balancing mechanism involving POF and HP1 that provides a feedback system for fine-tuning expression status of genes on the 4th chromosome...
  99. ncbi Structural and thermodynamic evidence for a stabilizing role of Nop5p in S-adenosyl-L-methionine binding to fibrillarin
    Mohamed Aittaleb
    Department of Chemistry and Biochemistry, Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA
    J Biol Chem 279:41822-9. 2004
    ..Finally, assessment of cofactor-binding thermodynamics and catalytic activities of enzyme mutants identified three additional fibrillarin residues (Thr-70, Glu-88, and Asp-133) to be important for cofactor binding and for catalysis...
  100. pmc Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus
    Suzana Hadjur
    Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
    Nature 460:410-3. 2009
    ..This new aspect of cohesin function is probably important for normal development and disease...
  101. pmc Painting of fourth, a chromosome-specific protein in Drosophila
    J Larsson
    Department of Microbiology, Umea University, S 901 87 Umea, Sweden
    Proc Natl Acad Sci U S A 98:6273-8. 2001
    ..In the related species Drosophila busckii, POF paints the entire X chromosome exclusively in males, suggesting relationships between the fourth chromosome and the X and between POF complexes and dosage-compensation complexes...

Research Grants62

  1. Human HMGN proteins: role in chromatin & gene function
    Barbara Rattner; Fiscal Year: 2007
    ..Together, these studies will contribute to the understanding of the biological functions of the HMGN proteins in the context of chromatin. ..
  2. Chromatin and Coactivators in HSV-1 gene regulation
    STEVEN TRIEZENBERG; Fiscal Year: 2009
    ..Early stages in virus infection seem to depend on certain enzymes from the host cell. This research will validate the roles of those enzymes in virus infection, which may lead to new antiviral drug targets. ..
  3. Post-translational Modifications of High-mobility Group Proteins
    Yinsheng Wang; Fiscal Year: 2009
    ..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
  4. MOLECULAR CYTOGENETICS OF HETEROCHROMATIN
    JOEL EISSENBERG; Fiscal Year: 1992
    ....
  5. DEVELOPMENTAL BIOLOGY OF HEMOGLOBIN SWITCHING
    George Stamatoyannopoulos; Fiscal Year: 1991
    ..This will have therapeutic implications in patients with globin disorders such as sickle cell anemia...
  6. Functions of a Novel SET Protein in KRAB/KAP1 Repression
    David Schultz; Fiscal Year: 2006
    ..It is our expectation that the proposed research will define KIS1 as the missing link to understanding heterochromatin mediated gene silencing by the KRAB:KAP-1 repression system. ..
  7. GENETIC-MOLECULAR BASIS OF DROSOPHILA LONGEVITY
    JAMES FRISTROM; Fiscal Year: 1980
    ....
  8. NONHISTONE CHROMOSOMAL PROTEINS AND HEPATOMA GROWTH
    Michael Lea; Fiscal Year: 1980
    ..The potential of proteins, which are either increased or decreased in amount in hepatomas, to exhibit contrasting proterties in these test systems will be investigated...
  9. HISTONE BIOCHEMISTRY AND THE INFLUENCE OF HORMONES
    R Cole; Fiscal Year: 1992
    ..differences (variants of H1 and other histones, histone and DNA modifications, kinds of DNA non histone chromosomal proteins)...
  10. EFFECT OF CANNABINOIDS ON THE GENOME OF MAMMALIAN CELLS
    Gary Stein; Fiscal Year: 1980
    ..These studies are being carried out in intact animals, with various tissues and cell types being examined and in cultured human cells, both quiescent and proliferating...
  11. Gene Activation by Remote Transcriptional Enhancers
    Dale Dorsett; Fiscal Year: 2004
    ..This will help illuminate the basic mechanisms underlying gene expression defects that occur in some human diseases. ..
  12. Functions of SWAP-70 in B Cell Activation
    Rolf Jessberger; Fiscal Year: 2006
    ..Therefore, the analysis of SWAP-70 in B cell activation is likely to help in our understanding of normal B cell function as well as of immune deficiencies, B cell malignancies, autoimmunity, and allergy. ..
  13. The Function of SWAP-70 in Mast Cells
    Rolf Jessberger; Fiscal Year: 2007
    ..The proposed experiments should yield key information on pathways regulating mast cell exocytosis, and will help understanding mast cell-associated diseases such as allergic reactions. ..
  14. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
  15. Biochemical Analysis of Sister Chromatid Cohesion
    Tatsuya Hirano; Fiscal Year: 2006
    ..The information obtained from this work will ultimately lead to a better understanding of human health because chromosome anomalies, such as aneuploidy and translocation, are tightly associated with tumor development and birth defects. ..
  16. BIOLOGY OF BAMACAN IN TUMORIGENESIS
    Giancarlo Ghiselli; Fiscal Year: 2006
    ..3) The contribution of bamacan in vivo to colon tumorigenesis by generating transgenic mice over-expressing bamacan in the intestinal epithelium. ..
  17. BIOCHEMICAL ANALYSIS OF MITOTIC CHROMOSOME CONDENSATION
    Tatsuya Hirano; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  18. Molecular mechanism of DNA damage recognition in mammals
    Kyoko Yokomori; Fiscal Year: 2008
    ..abstract_text> ..
  19. Analysis of the Novel Tumor Suppressor Gene SNF5/INI1
    Charles Roberts; Fiscal Year: 2006
    ..This proposal directly addresses scientific priorities identified by the BT-PRG arm of the NCI. ..
  20. Role of Human Condensin in DNA repair
    Kyoko Yokomori; Fiscal Year: 2006
    ..I believe that the project will make a significant contribution to our understanding of genome maintenance in human cells. ..
  21. Biochemical Basis of CENP-A Epigenetic Inheritance
    SAMANTHA ZEITLIN; Fiscal Year: 2005
    ..These studies will also have direct implications for understanding cancer causation, and applications in gene therapy. ..
  22. Centrosome Amplification in Human Breast Cancer
    JEFFREY SALISBURY; Fiscal Year: 2006
    ..The study of centrosome behavior is of fundamental importance to our understanding of the origin of malignant tumors and may reveal new targets for intervention or prevention of the development of breast cancer. ..
  23. FASEB Summer Conference, July 20-25, 2002
    Fuyuhiko Tamanoi; Fiscal Year: 2002
    ..Our unique focus on lipid rafts and their significance in signal transduction as well as focus on cancer therapy and anti-parasite strategies promises to bring together a wide range of researchers. ..
  24. HUMAN SMC FAMILY PROTEINS IN CHROMOSOME DYNAMICS
    Kyoko Yokomori; Fiscal Year: 2003
    ..abstract_text> ..
  25. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
  26. NEGATIVE REGULATION OF CELL CYCLE PROGRESSION
    M Hoyt; Fiscal Year: 2004
    ..The identified genes will report checkpoint activation and will allow a powerful dissection of this signal transduction pathway. ..
  27. MULTIDISCIPLINARY BASIC RESEARCH TRAINING IN CANCER
    JEFFREY SALISBURY; Fiscal Year: 2002
    ..Separate facilities are available for animal housing, engineering, and classroom and lecture space. ..
  28. Telomerase function and regulation in a new model system
    Neal Lue; Fiscal Year: 2007
    ..We anticipate that our findings will contribute to a detailed molecular understanding of telomerase function and regulation. ..
  29. The control of growth and metabolism by effectors of the TOR signaling pathway
    Michael Lehmann; Fiscal Year: 2007
    ..It will thus improve our under- standing of how nutrient sensing is linked to fat metabolism and help paving the way for treatments of obesity-related (type 2) diabetes and other diseases. [unreadable] [unreadable] [unreadable]..
  30. Mechanism of Mitotic Checkpoint in Mammalian Cells
    TIMOTHY YEN; Fiscal Year: 2007
    ..2 Description, ..
  31. HIV Impairs Reverse Cholesterol Transport
    Michael Bukrinsky; Fiscal Year: 2006
    ..These studies are expected to yield sufficient information upon which to base a well-planned and rigorous series of further investigations. [unreadable] [unreadable]..
  32. Chemical Genetic and Biochmechical Studies of Mitotic Proteolysis
    RANDALL KING; Fiscal Year: 2007
    ..abstract_text> ..
  33. Epigenetic Etiologies of Autism-Spectrum Disorders
    Janine LaSalle; Fiscal Year: 2009
    ..The successful completion of these studies should yield findings to help develop better diagnostic and treatment modalities for autism-spectrum disorders. ..
  34. A Novel HTP Assay for Identification of Compounds that Reduce PNC Prevalence
    Sui Huang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  35. Intraflagellar Transport Motors in C. elegans neurons
    Jonathan M Scholey; Fiscal Year: 2010
    ....
  36. Role of PML nuclear body proteins for establishment of EBV latent infection
    PAUL DALLING LING; Fiscal Year: 2010
    ..Design of EBNA2 and/or EBNA-LP inhibitors is a potential practical application that will be derived from our studies. ..
  37. READTHROUGH ACTIVATION OF GENE TRANSCRIPTION
    David Spector; Fiscal Year: 2001
    ..Understanding new molecular mechanisms for controlling gene expression has applications in the development of viral vectors and cellular expression systems for gene therapy. ..
  38. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  39. MOLECULAR REGULATION OF FXR ACTIVITY
    Jongsook Kim Kemper; Fiscal Year: 2010
    ..The studies may also facilitate the design of therapeutic agents for treating these metabolic disorders. ..
  40. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal F Lue; Fiscal Year: 2010
    ....
  41. Mechanism of in-vitro aging
    LYSLE LEWIS; Fiscal Year: 2007
    ..The proposed research will enhance our understanding of events that occur as a consequence of shortened chromosomes and factors that affect the rate of shortening. [unreadable] [unreadable] [unreadable]..
  42. Smt4 Regulation of Centromere Function and Cohesion
    Jeff Bachant; Fiscal Year: 2007
    ..These studies will elucidate potentially conserved mechanisms controlling chromosome segregation and identify additional Smt3/SUMO-1 substrates regulating centromere function. ..
  43. MERCURY INDUCED AUTOIMMUNITY
    Kenneth Pollard; Fiscal Year: 2007
    ..Identification of the role that IFN-gamma plays in the development of induced murine systemic autoimmunity should prove applicable to murine models of idiopathic systemic autoimmunity and to human lupus. ..
  44. New Inhibitors of HIV Replication
    Gabriele Varani; Fiscal Year: 2005
    ..While the project is challenging and partly untested, the introduction of new structures leading to therapeutic options for treating drug-resistant viruses would have substantial impact. ..
  45. C-X-C Chemokines and Kaposi's Sarcoma
    DAVID MARKOVITZ; Fiscal Year: 2005
    ..This work thus has the potential to link KSHV, growth factors, and HIV/Tat in the pathogenesis of KS, and to suggest new avenues for the treatment of this complex neoplasm. ..
  46. Specification of Mammalian Replication Origins
    David Gilbert; Fiscal Year: 2005
    ..abstract_text> ..
  47. NUCLEOPROTEINS IN MAMMALIAN SPERMATOGENESIS
    Marvin Meistrich; Fiscal Year: 2006
    ..Thus they provide a well-defined model system for study of human infertility. ..
  48. IMMUNOTOXICOLOGY OF A HEAVY METAL
    Kenneth Pollard; Fiscal Year: 2004
    ..Analysis of the interaction between fibrillarin, mercury and cells of the lymphoid system may lead to insights into how an imunotoxin renders self-antigen immunogenic. ..
  49. Structure Determination of the Polymerase alpha-primase
    DEWIGHT WILLIAMS; Fiscal Year: 2006
    ..abstract_text> ..
  50. NUCLEAR TRANSLOCATION OF HIV 1 GENOME AS A DRUG TARGET
    Michael Bukrinsky; Fiscal Year: 2004
    ..Upon completion, these studies are expected to define potent anti-HIV compounds working through a novel mechanism different from that of any other currently used drug. ..
  51. XENOBIOTIC ACCELERATION OF AUTOIMMUNITY
    Kenneth Pollard; Fiscal Year: 2003
    ..abstract_text> ..
  52. PERINUCLEOLAR COMPARTMENT AND CANCER
    Sui Huang; Fiscal Year: 2003
    ..The results of these experiments will provide an important foundation to address the significance of the PNC in malignant cells. ..
  53. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..
  54. MHC-Defined and Identical Twin Monkeys for Research
    Joseph Kemnitz; Fiscal Year: 2005
    ..Additionally, genetically identical macaques will have general utility in biomedical research, especially to the neuroscience community. ..
  55. 2006 Biophysical Discussions - Molecuar Motors: Point Counterpoint
    Steven Rosenfeld; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  56. Role of MeCP2 as a Chromatin Architectural Protein
    CHRISTOPHER WOODCOCK; Fiscal Year: 2007
    ..The results will provide fundamental new information of the interactions of MeCP2 with chromatin, information that can be drawn on as potential therapies for RTT are formulated and evaluated. ..
  57. 750 Mhz NMR Console
    Gabriele Varani; Fiscal Year: 2005
    ..Furthermore, the new console will reduce the inevitable downtime associated with console failure, which are becoming increasingly common as the spectrometer ages. ..
  58. Regulation of rRNA Genes by Silencing Mechanisms
    Danesh Moazed; Fiscal Year: 2010
    ....