microtubule associated proteins

Summary

Summary: High molecular weight proteins found in the MICROTUBULES of the cytoskeletal system. Under certain conditions they are required for TUBULIN assembly into the microtubules and stabilize the assembled microtubules.

Top Publications

  1. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
  2. ncbi Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice
    Taichi Hara
    Department of Bioregulation and Metabolism, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    Nature 441:885-9. 2006
  3. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
  4. pmc Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice
    Masaaki Komatsu
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    J Cell Biol 169:425-34. 2005
  5. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
  6. pmc Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells
    E Smirnova
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California 90095 1737, USA
    Mol Biol Cell 12:2245-56. 2001
  7. ncbi The role of autophagy during the early neonatal starvation period
    Akiko Kuma
    Time s Arrow and Biosignaling, PRESTO, Japan Science and Technology Agency, Kawaguchi 332 0012, Japan
    Nature 432:1032-6. 2004
  8. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
  9. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
  10. ncbi Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice
    Masaaki Komatsu
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Cell 131:1149-63. 2007

Research Grants

  1. Small Animal Model of HIV Neuropathogenesis in the HAART Era
    Pietro P Sanna; Fiscal Year: 2012
  2. A kinase pathway required for cortical neuron polarization
    Franck Polleux; Fiscal Year: 2012
  3. Novel Roles of Intracrine Angiotensin II in Proximal Tubule Cells
    Jia Zhuo; Fiscal Year: 2009
  4. Kevin C Slep; Fiscal Year: 2014
  5. Role of msps and tacc during axon guidance
    LAURA A LOWERY; Fiscal Year: 2010
  6. Microtubule Stability in Lung Endothelium
    CRISTHIAAN D OCHOA ARENAS; Fiscal Year: 2012
  7. PEROXYNITRITE DAMAGE TO MICROTUBULE PROTEINS
    LISA MARIE LANDINO; Fiscal Year: 2011
  8. Rick B Meeker; Fiscal Year: 2016
  9. Angela N Carter; Fiscal Year: 2014
  10. Gloria Lee; Fiscal Year: 2016

Detail Information

Publications408 found, 100 shown here

  1. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
    ..The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes...
  2. ncbi Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice
    Taichi Hara
    Department of Bioregulation and Metabolism, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    Nature 441:885-9. 2006
    ....
  3. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
    ..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response...
  4. pmc Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice
    Masaaki Komatsu
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    J Cell Biol 169:425-34. 2005
    ..Our results indicate the important role of autophagy in starvation response and the quality control of proteins and organelles in quiescent cells...
  5. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
    ..We suggest that p62 may, via LC3, be involved in linking polyubiquitinated protein aggregates to the autophagy machinery...
  6. pmc Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells
    E Smirnova
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California 90095 1737, USA
    Mol Biol Cell 12:2245-56. 2001
    ..We conclude that Drp1 contributes to mitochondrial division in mammalian cells...
  7. ncbi The role of autophagy during the early neonatal starvation period
    Akiko Kuma
    Time s Arrow and Biosignaling, PRESTO, Japan Science and Technology Agency, Kawaguchi 332 0012, Japan
    Nature 432:1032-6. 2004
    ..These results suggest that the production of amino acids by autophagic degradation of 'self' proteins, which allows for the maintenance of energy homeostasis, is important for survival during neonatal starvation...
  8. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
    ..This method will be of general utility for analysis of the autophagosome maturation process...
  9. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
    ..Remarkably, inhibition of Drp1 blocks cell death, implicating mitochondrial fission as an important step in apoptosis...
  10. ncbi Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice
    Masaaki Komatsu
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Cell 131:1149-63. 2007
    ....
  11. ncbi A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma
    G Ambrosini
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nat Med 3:917-21. 1997
    ..These findings suggest that apoptosis inhibition may be a general feature of neoplasia and identify survivin as a potential new target for apoptosis-based therapy in cancer and lymphoma...
  12. ncbi A ubiquitin-like system mediates protein lipidation
    Y Ichimura
    Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
    Nature 408:488-92. 2000
    ..11) and Apg3, respectively. These reactions are necessary for the formation of Apg8-phosphatidylethanolamine. This lipidation has an essential role in membrane dynamics during autophagy...
  13. pmc Fission and selective fusion govern mitochondrial segregation and elimination by autophagy
    Gilad Twig
    Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, MA 02111, USA
    EMBO J 27:433-46. 2008
    ..Together, these findings suggest that fission followed by selective fusion segregates dysfunctional mitochondria and permits their removal by autophagy...
  14. ncbi IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs
    I Tamm
    The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 58:5315-20. 1998
    ..Although quantitative differences may exist, these observations suggest commonality in the mechanisms used by IAP-family proteins to suppress apoptosis...
  15. doi Tracking the ends: a dynamic protein network controls the fate of microtubule tips
    Anna Akhmanova
    Department of Cell Biology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Rev Mol Cell Biol 9:309-22. 2008
    ..TIPs regulate different aspects of cell architecture by controlling microtubule dynamics, microtubule interactions with cellular structures and signalling factors, and the forces that are exerted on microtubule networks...
  16. ncbi Doublecortin expression levels in adult brain reflect neurogenesis
    Sebastien Couillard-Despres
    Volkswagen Foundation Junior Group, University of Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
    Eur J Neurosci 21:1-14. 2005
    ....
  17. ncbi Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons
    F Francis
    U129 de l INSERM, Institut Cochin de Genetique Moleculaire, Paris
    Neuron 23:247-56. 1999
    ..Our data suggest that the cortical dysgeneses associated with the loss of Doublecortin function might result from abnormal cytoskeletal dynamics in neuronal cell development...
  18. ncbi Control of apoptosis and mitotic spindle checkpoint by survivin
    F Li
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 396:580-4. 1998
    ..The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis...
  19. pmc Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells
    N Mizushima
    Unit Process and Combined Circuit, PRESTO, Japan Science and Technology Corporation, Kawaguchi 332 0012, Japan
    J Cell Biol 152:657-68. 2001
    ..We also show that Apg12-Apg5 is required for targeting of a mammalian Aut7/Apg8 homologue, LC3, to the isolation membranes. These results suggest that the Apg12-Apg5 conjugate plays essential roles in isolation membrane development...
  20. ncbi Transient expression of doublecortin during adult neurogenesis
    Jason P Brown
    Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
    J Comp Neurol 467:1-10. 2003
    ..This assumption is further supported by our observation that the number of DCX-expressing cells in the dentate gyrus was decreased with age according to the reduction of neurogenesis in the aging dentate gyrus previously reported...
  21. ncbi Validating survivin as a cancer therapeutic target
    Dario C Altieri
    Department of Cancer Biology, LRB 428, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nat Rev Cancer 3:46-54. 2003
    ..Survivin is an attractive candidate for cancer therapy, so what is its potential applicability in the clinic?..
  22. ncbi Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons
    J G Gleeson
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
    Neuron 23:257-71. 1999
    ..Therefore, DCX likely directs neuronal migration by regulating the organization and stability of microtubules...
  23. doi Survivin: key regulator of mitosis and apoptosis and novel target for cancer therapeutics
    Alain C Mita
    Institute for Drug Development, Cancer Therapy and Research Center at the University of Texas Health Science Center, 7979 Wurzbach Road, San Antonio, TX 78229, USA
    Clin Cancer Res 14:5000-5. 2008
    ..This article reviews the multiple functions of survivin in the regulation of apoptosis, the promotion of tumorigenesis, and the development of survivin inhibitors as a novel anticancer therapeutic strategy...
  24. ncbi LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation
    Yukiko Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Myodaiji 38, Okazaki 444 8585, Japan
    J Cell Sci 117:2805-12. 2004
    ..Generation of the form II correlates with autophagosome association of GABARAP and GATE16. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II...
  25. ncbi p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy
    Serhiy Pankiv
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 282:24131-45. 2007
    ..In fact, p62 bodies and these structures are indistinguishable. Taken together, our results clearly suggest that p62 is required both for the formation and the degradation of polyubiquitin-containing bodies by autophagy...
  26. doi An EB1-binding motif acts as a microtubule tip localization signal
    Srinivas Honnappa
    Biomolecular Research, Structural Biology, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland
    Cell 138:366-76. 2009
    ..Our findings establish a general "microtubule tip localization signal" (MtLS) and delineate a unifying mechanism for this subcellular protein targeting process...
  27. ncbi The inhibitors of apoptosis (IAPs) and their emerging role in cancer
    E C LaCasse
    Apoptogen Inc, CHEO Research Institute, Ottawa, Ontario, Canada
    Oncogene 17:3247-59. 1998
    ..Overall a picture consistent with an IAP role in tumour progression rather than tumour initiation is emerging making the IAPs an attractive therapeutic target...
  28. pmc Formation process of autophagosome is traced with Apg8/Aut7p in yeast
    T Kirisako
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 147:435-46. 1999
    ..Moreover, it is revealed that autophagosome formation is severely impaired in the apg8 null mutant. Apg8p would play an important role in the autophagosome formation...
  29. pmc The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
    T Kirisako
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 151:263-76. 2000
    ..A reaction similar to ubiquitination is probably involved in the second modification. The reversible modification of Apg8 appears to be coupled to the membrane dynamics of autophagy and the Cvt pathway...
  30. pmc Assembly of centrosomal proteins and microtubule organization depends on PCM-1
    Alexander Dammermann
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK
    J Cell Biol 159:255-66. 2002
    ....
  31. pmc Mammalian end binding proteins control persistent microtubule growth
    Yulia Komarova
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, USA
    J Cell Biol 184:691-706. 2009
    ..This probably occurs through their action on microtubule ends, because catastrophe suppression does not require the EB domains needed for binding to known EB partners...
  32. ncbi Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
    Tatiana Stepanova
    Medical Genetics Center Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands
    J Neurosci 23:2655-64. 2003
    ..We propose that similar mechanisms underlie the specific association of CLIPs and EB1-related proteins with the ends of growing microtubules in non-neuronal and neuronal cells...
  33. pmc Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division
    Jessica Yingling
    Departments of Pediatrics and Medicine, Center for Human Genetics and Genomics, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92098 0627, USA
    Cell 132:474-86. 2008
    ..Thus, control of symmetric division, essential for neuroepithelial stem cell proliferation, is mediated through spindle orientation determined via LIS1/NDEL1/dynein-mediated cortical microtubule capture...
  34. ncbi The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus
    Christian J Malone
    Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Cell 115:825-36. 2003
    ..We propose that dynein and ZYG-12 move the centrosomes toward the nucleus, followed by a ZYG-12/SUN-1-dependent anchorage...
  35. ncbi Reconstitution of a microtubule plus-end tracking system in vitro
    Peter Bieling
    European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 450:1100-5. 2007
    ..We expect that such in vitro reconstitutions will also be essential for the mechanistic dissection of other plus-end tracking systems...
  36. ncbi Transcriptional repression of the anti-apoptotic survivin gene by wild type p53
    William H Hoffman
    Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 277:3247-57. 2002
    ..The identification of survivin as a p53 repressed gene should aid in the elucidation of the contribution of transcriptional repression to p53-dependent apoptosis...
  37. pmc Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
    Kazuhisa Kinoshita
    Max Planck Institute of Molecular Cell Biology and Genetics MPI CBG, 01307 Dresden, Germany
    J Cell Biol 170:1047-55. 2005
    ..We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis...
  38. ncbi DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation
    Jill A Morris
    Department of Neuroscience, Merck Research Laboratories, West Point, PA 19486, USA
    Hum Mol Genet 12:1591-608. 2003
    ....
  39. pmc Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
    Elizabeth L Axe
    Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
    J Cell Biol 182:685-701. 2008
    ..This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation...
  40. ncbi Kinesin and dynein superfamily proteins and the mechanism of organelle transport
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Tokyo, Japan
    Science 279:519-26. 1998
    ..This review focuses on the molecular mechanism of organelle transport in cells and describes kinesin and dynein superfamily proteins...
  41. pmc The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line
    Gohta Goshima
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1003-16. 2003
    ..From our phenotypic data, we construct a model for the distinct roles of molecular motors during mitosis in a single metazoan cell type...
  42. pmc Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function
    Srinivas Venkatram
    Department of Cell and Developmental Biology, Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Biol Cell 15:2287-301. 2004
    ..This study expands the known roles of gamma-tubulin complex components in organizing different types of microtubule structures in S. pombe...
  43. ncbi Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology
    Chuang Rung Chang
    Cellular Neurology Unit, NINDS, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:21583-7. 2007
    ..Thus, protein phosphorylation at Ser(637) results in clear alterations in Drp1 function and mitochondrial morphology that are likely involved in dynamic regulation of mitochondrial division in cells...
  44. ncbi Survivin and molecular pathogenesis of colorectal cancer
    Paul J Kim
    Department of Cancer Biology and the Cancer Center, University of Massachusetts, Medical School, Worcester, MA, USA
    Lancet 362:205-9. 2003
    ..Survivin is a bifunctional regulator of cell death and cell proliferation expressed during embryonic development but undetectable in healthy adult tissues and re-expressed in many cancers, including colorectal cancer...
  45. ncbi The case for survivin as a regulator of microtubule dynamics and cell-death decisions
    Dario C Altieri
    Department of Cancer Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Opin Cell Biol 18:609-15. 2006
    ..Building a unifying model to reconcile the differing views on survivin will aid in the design and interpretation of molecularly based clinical trials targeting this network in humans...
  46. ncbi EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration
    Ying Wen
    Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA
    Nat Cell Biol 6:820-30. 2004
    ..These results show an evolutionarily conserved pathway for microtubule capture, and suggest that mDia functions as a scaffold protein for EB1 and APC to stabilize microtubules and promote cell migration...
  47. ncbi Chromosomal passengers: conducting cell division
    Sandrine Ruchaud
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, King s Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK
    Nat Rev Mol Cell Biol 8:798-812. 2007
    ....
  48. ncbi Escape of intracellular Shigella from autophagy
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Science 307:727-31. 2005
    ..Rather, Shigella VirG, a protein required for intracellular actin-based motility, induced autophagy by binding to the autophagy protein, Atg5. In nonmutant Shigella, this binding is competitively inhibited by IcsB binding to VirG...
  49. ncbi Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion
    Hitoshi Nakatogawa
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Cell 130:165-78. 2007
    ..Our results provide further insights into the mechanisms underlying the unique membrane dynamics of autophagy and also indicate the functional versatility of ubiquitin-like proteins...
  50. pmc The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells
    Fanni Gergely
    Department of Pharmacology, University of Cambridge, Wellcome Trust Cancer Research UK Institute, Cambridge CB2 1QR, UK
    Genes Dev 17:336-41. 2003
    ..Thus, in human somatic cells, ch-TOG appears to play a major role in organizing spindle poles, and a more minor role in stabilizing spindle microtubules that is, at least in part, mediated via an interaction with TACC3...
  51. ncbi An IAP-IAP complex inhibits apoptosis
    Takehiko Dohi
    Department of Cancer Biology and the Cancer Center, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    J Biol Chem 279:34087-90. 2004
    ..Therefore, orchestration of an IAP-IAP complex regulates apoptosis...
  52. ncbi Mitochondrial bioenergetics and structural network organization
    Giovanni Benard
    Institut National de la Sante et de la Recherche Medicale INSERM, U688 Physiopathologie Mitochondriale, Universite Victor Segalen Bordeaux 2, 146 rue Leo Saignat, F 33076 Bordeaux cedex, France
    J Cell Sci 120:838-48. 2007
    ..Our data demonstrate that the relationship between mitochondrial network organization and bioenergetics is bidirectional, and we provide a model for analyzing the metabolic signals involved in this crosstalk...
  53. doi Structural basis for sorting mechanism of p62 in selective autophagy
    Yoshinobu Ichimura
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421
    J Biol Chem 283:22847-57. 2008
    ..These results demonstrate that the intracellular level of p62 is tightly regulated by autophagy through the direct interaction of LC3 with p62 and reveal that selective turnover of p62 via autophagy controls inclusion body formation...
  54. ncbi Graded reduction of Pafah1b1 (Lis1) activity results in neuronal migration defects and early embryonic lethality
    S Hirotsune
    Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 19:333-9. 1998
    ..The phenotypes observed are distinct from those of other mouse mutants with neuronal migration defects, suggesting that Pafah1b1 participates in a novel pathway for neuronal migration...
  55. ncbi Genetic interactions between doublecortin and doublecortin-like kinase in neuronal migration and axon outgrowth
    Thomas A S Deuel
    Howard Hughes Medical Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 49:41-53. 2006
    ..Dcx and Dclk may directly or indirectly regulate microtubule-based vesicle transport, a process critical to both neuronal migration and axon outgrowth...
  56. pmc Dynamics of inner kinetochore assembly and maintenance in living cells
    Peter Hemmerich
    Leibniz Institute for Age Research, Fritz Lipmann Institute, 07745 Jena, Germany i leibniz de
    J Cell Biol 180:1101-14. 2008
    ..Our data reveal a wide range of cell cycle-specific assembly plasticity of the centromere that provides both stability through sustained binding of some components and flexibility through dynamic exchange of other components...
  57. pmc Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued
    K T Vaughan
    Cell Biology Group, Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, USA
    J Cell Biol 131:1507-16. 1995
    ..Our data also suggest, however, that this interaction must be highly regulated...
  58. pmc Roles of Hof1p, Bni1p, Bnr1p, and myo1p in cytokinesis in Saccharomyces cerevisiae
    E A Vallen
    Department of Biology, Swarthmore College, Swarthmore, Pennsylvania 19081, USA
    Mol Biol Cell 11:593-611. 2000
    ..In bni1Delta cells that lack the actomyosin contraction, septum formation is often slow and asymmetric, suggesting that actomyosin contraction may provide directionality for efficient septum formation...
  59. ncbi Cdk5 phosphorylation of doublecortin ser297 regulates its effect on neuronal migration
    Teruyuki Tanaka
    Department of Neurosciences, University of California, San Diego, La Jolla, 92093, USA
    Neuron 41:215-27. 2004
    ..These results suggest that Dcx phosphorylation by Cdk5 regulates its actions on migration through an effect on microtubules...
  60. ncbi CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta
    Gideon Lansbergen
    MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Dev Cell 11:21-32. 2006
    ..We propose that LL5beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends...
  61. ncbi Regulation of survivin expression by IGF-1/mTOR signaling
    V Vaira
    Department of Cancer Biology and the Cancer Center, University of Massachusetts Medical School, Worcester, MA 01605, USA, and AOS Paolo and Fondazione Ospedale Maggiore Policlinico, Regina Elena and Mangiagalli, Milan, Italy
    Oncogene 26:2678-84. 2007
    ..Antagonists of this pathway may be beneficial to lower an antiapoptotic threshold maintained by survivin in prostate cancer...
  62. ncbi Doublecortin expression in the adult rat telencephalon
    J Nacher
    Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY 10021, USA
    Eur J Neurosci 14:629-44. 2001
    ..Given these results, we hypothesize that DCX expression in differentiated neurons could be related to its capacity for microtubule reorganization and that this fact could be linked to axonal outgrowth or synaptogenesis...
  63. ncbi Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
    ..Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization...
  64. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  65. ncbi Dual subcellular roles for LIS1 and dynein in radial neuronal migration in live brain tissue
    Jin Wu Tsai
    Integrated Program in Cellular, Molecular and Biophysical Studies, Columbia University, 630 W 168th Street, New York, New York 10032, USA
    Nat Neurosci 10:970-9. 2007
    ..Thus, dynein pulls on the microtubule network from the swelling. The nucleus is transported along the trailing microtubules by dynein assisted by myosin II...
  66. doi A role for NBR1 in autophagosomal degradation of ubiquitinated substrates
    Vladimir Kirkin
    Institute of Biochemistry II, Goethe University, Frankfurt Main, Germany
    Mol Cell 33:505-16. 2009
    ..We propose that NBR1 and p62 act as receptors for selective autophagosomal degradation of ubiquitinated targets...
  67. ncbi Escaping cell death: survival proteins in cancer
    M Jaattela
    Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, Copenhagen, DK 2100, Denmark
    Exp Cell Res 248:30-43. 1999
    ..Understanding the molecular mechanisms of action of these proteins may offer novel modes of rationally and selectively manipulating the sensitivity of cancer cells to therapy...
  68. pmc Defects in axonal elongation and neuronal migration in mice with disrupted tau and map1b genes
    Y Takei
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Cell Biol 150:989-1000. 2000
    ..These findings indicate the cooperative functions of tau and MAP1B in vivo in axonal elongation and neuronal migration as regulators of microtubule organization...
  69. ncbi Cdc42, dynein, and dynactin regulate MTOC reorientation independent of Rho-regulated microtubule stabilization
    A F Palazzo
    Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA
    Curr Biol 11:1536-41. 2001
    ..MTOC reorientation and MT stabilization both act to polarize the MT array in migrating cells, yet these processes act independently and are regulated by separate Rho family GTPase-signaling pathways...
  70. pmc The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. Microtubule-associated proteins
    Scott C Schuyler
    Department of Pediatric Oncology, The Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 160:517-28. 2003
    ..We propose that Ase1p functions as an MT cross-bridge that imparts matrix-like characteristics to the midzone. MT-dependent networks of spindle midzone MAPs may be one molecular basis for the postulated spindle matrix...
  71. ncbi APC binds to the novel protein EB1
    L K Su
    Johns Hopkins Oncology Center, Baltimore, Maryland 21231, USA
    Cancer Res 55:2972-7. 1995
    ..This conservation suggests an essential function for EB1 that might provide clues to the mechanism through which APC suppresses colonic neoplasia...
  72. pmc Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo
    P GONCZY
    European Molecular Biology Laboratory, Heidelberg, D 69117 Germany
    J Cell Biol 147:135-50. 1999
    ..In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation...
  73. pmc A role for survivin in chemoresistance of endothelial cells mediated by VEGF
    Jennifer Tran
    Molecular and Cellular Biology Research, Sunnybrook and Women s College Health Sciences Centre, Toronto, ON, Canada M4N 3M5
    Proc Natl Acad Sci U S A 99:4349-54. 2002
    ....
  74. ncbi Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170
    Masaki Fukata
    Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Cell 109:873-85. 2002
    ..These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization...
  75. pmc Mitochondrial survivin inhibits apoptosis and promotes tumorigenesis
    Takehiko Dohi
    Department of Cancer Biology and the Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Clin Invest 114:1117-27. 2004
    ..Therefore, mitochondrial survivin orchestrates a novel pathway of apoptosis inhibition, which contributes to tumor progression...
  76. pmc The mitochondrial protein hFis1 regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1
    Yisang Yoon
    Center for Basic Research in Digestive Diseases and Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA
    Mol Cell Biol 23:5409-20. 2003
    ..We propose that hFis1 is a limiting factor in mitochondrial fission and that the number of hFis1 molecules on the mitochondrial surface determines fission frequency...
  77. ncbi Translational control of p27Kip1 accumulation during the cell cycle
    L Hengst
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 271:1861-4. 1996
    ....
  78. ncbi The conserved KMN network constitutes the core microtubule-binding site of the kinetochore
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East, Room 3052, La Jolla, CA 92093, USA
    Cell 127:983-97. 2006
    ..Based on these findings, we propose that the conserved KMN network constitutes the core microtubule-binding site of the kinetochore...
  79. ncbi Survivin, cancer networks and pathway-directed drug discovery
    Dario C Altieri
    Department of Cancer Biology and the Cancer Center, Lazare Research Building 428, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nat Rev Cancer 8:61-70. 2008
    ..Pursuing the nodal functions of survivin in cancer might lead to the development of global pathway inhibitors with unique therapeutic potential...
  80. pmc Atg8 controls phagophore expansion during autophagosome formation
    Zhiping Xie
    Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:3290-8. 2008
    ..This model provides a foundation for future analyses of the functions and dynamics of known autophagy-related proteins and for screening new genes...
  81. ncbi The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3
    I Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Biol Chem 276:1701-6. 2001
    ..These results indicate that hApg7p is an authentic protein-activating enzyme for hApg12p and the three Apg8p homologs...
  82. ncbi Inhibition of STAT3 signaling induces apoptosis and decreases survivin expression in primary effusion lymphoma
    Yoshiyasu Aoki
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:1535-42. 2003
    ..Since constitutive STAT3 activation and survivin expression have been widely documented in different types of cancers, their linkage may extend to many malignancies and be critical to their pathogenesis...
  83. pmc The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization
    Lee A Ligon
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6085, USA
    Mol Biol Cell 14:1405-17. 2003
    ..Differences in the expression and regulation of plus-end proteins in different cell types may underlie previously noted differences in microtubule dynamics...
  84. ncbi Survivin, a cancer target with an emerging role in normal adult tissues
    Seiji Fukuda
    Walther Oncology Center, Indiana University School of Medicine, 950 West Walnut Street, Indianapolis, IN 46202, USA
    Mol Cancer Ther 5:1087-98. 2006
    ....
  85. pmc Inhibition of macroautophagy triggers apoptosis
    Patricia Boya
    CNRS UMR 8125, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Mol Cell Biol 25:1025-40. 2005
    ..All together, these data indicate that autophagy may be cytoprotective, at least under conditions of nutrient depletion, and point to an important cross talk between type 1 and type 2 cell death pathways...
  86. ncbi Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B
    S S Mann
    Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Winston Salem, North Carolina 27157 1010
    J Biol Chem 269:11492-7. 1994
    ..We propose that LC3 functions primarily as a MAP1A and MAP1B subunit and that its expression could regulate the microtubule binding activity of MAP1A and MAP1B...
  87. ncbi The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules
    Y Mimori-Kiyosue
    Tsukita Cell Axis Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Shimogyo ku, 600 8813, Japan
    Curr Biol 10:865-8. 2000
    ..These findings showed that the APC-EB1 interaction is regulated within cells and is allowed near the ends of microtubules only under restricted conditions...
  88. pmc The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins
    J H Walenta
    Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Cell Biol 152:923-34. 2001
    ..Because overexpression of Hook3 caused fragmentation of the Golgi complex, we propose that Hook3 participates in defining the architecture and localization of the mammalian Golgi complex...
  89. pmc HBXIP functions as a cofactor of survivin in apoptosis suppression
    Hiroyuki Marusawa
    The Burnham Institute, La Jolla, CA 92037, USA
    EMBO J 22:2729-40. 2003
    ..Thus, HBXIP functions as a cofactor for survivin, and serves as a link between the cellular apoptosis machinery and a viral pathogen involved in hepatocellular carcinogenesis...
  90. pmc Analysis of the dynein-dynactin interaction in vitro and in vivo
    Stephen J King
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Mol Biol Cell 14:5089-97. 2003
    ..The dynein IC fragments have different effects on endomembrane localization, suggesting that different endomembranes may bind dynein via distinct mechanisms...
  91. pmc EB1 and EB3 control CLIP dissociation from the ends of growing microtubules
    Yulia Komarova
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, USA
    Mol Biol Cell 16:5334-45. 2005
    ....
  92. ncbi Human IRGM induces autophagy to eliminate intracellular mycobacteria
    Sudha B Singh
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Science 313:1438-41. 2006
    ..We also identified a function for a human IRG protein in the control of intracellular pathogens and report that the human Irgm1 ortholog, IRGM, plays a role in autophagy and in the reduction of intracellular bacillary load...
  93. ncbi Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast
    Marcel E Janson
    Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell 128:357-68. 2007
    ..By combining motor and bundling proteins, cells can thus dynamically organize stable regions of overlap between cytoskeletal filaments...
  94. ncbi Phosphorylation of microtubule-associated protein 2 (MAP2) and its relevance for the regulation of the neuronal cytoskeleton function
    C Sanchez
    Centro de Biologia Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autónoma de Madrid CSIC UAM, Cantoblanco, 28049, Madrid, Spain
    Prog Neurobiol 61:133-68. 2000
    ..Here we will review the current knowledge about the regulation of MAP2 function through phosphorylation/dephosphorylation and its relevance in the broader context of neuronal functions...
  95. ncbi Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype
    A G Uren
    The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, 3050, Victoria, Australia
    Curr Biol 10:1319-28. 2000
    ..To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice...
  96. ncbi Evidence that APC regulates survivin expression: a possible mechanism contributing to the stem cell origin of colon cancer
    T Zhang
    Division of Genetic and Preventive Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Cancer Res 61:8664-7. 2001
    ....
  97. ncbi RNAi reveals doublecortin is required for radial migration in rat neocortex
    Jilin Bai
    Department of Physiology and Neurobiology, University of Connecticut, 3107 Horsebarn Hill Road, U 4156, Storrs, Connecticut 06269, USA
    Nat Neurosci 6:1277-83. 2003
    ..In utero RNAi can therefore be effectively used to study the specific cellular roles of DCX in neocortical development and to produce an animal model of double cortex syndrome...
  98. ncbi Survivin, versatile modulation of cell division and apoptosis in cancer
    Dario C Altieri
    Department of Cancer Biology and the Cancer Center, LRB 428, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Oncogene 22:8581-9. 2003
    ....
  99. ncbi Microtubule nucleation: gamma-tubulin and beyond
    Christiane Wiese
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Cell Sci 119:4143-53. 2006
    ....
  100. ncbi Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein
    A B Bowman
    Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive La Jolla, CA 92093, USA
    Cell 103:583-94. 2000
    ..We propose that SYD mediates the axonal transport of at least one class of vesicles by interacting directly with KLC...
  101. pmc Human TPX2 is required for targeting Aurora-A kinase to the spindle
    Thomas A Kufer
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Biol 158:617-23. 2002
    ..We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly...

Research Grants65

  1. Small Animal Model of HIV Neuropathogenesis in the HAART Era
    Pietro P Sanna; Fiscal Year: 2012
    ..Ultimately, the proposed Tg mice will allow mechanistic studies aimed at the generation of new pathogenetic hypotheses of HIV-associated neurocognitive disorders in the setting of HAART and their interaction with drugs of abuse. ..
  2. A kinase pathway required for cortical neuron polarization
    Franck Polleux; Fiscal Year: 2012
    ..for the ability of LKB1 to specify neuronal polarity in part through their ability to phosphorylate microtubule associated proteins (MAPs) such as Tau...
  3. Novel Roles of Intracrine Angiotensin II in Proximal Tubule Cells
    Jia Zhuo; Fiscal Year: 2009
    ..These studies will provide new insights into important roles of intracellular or intracrine Ang II in the regulation of salt and fluid transport in proximal tubules of the kidney and in the pathogenesis of hypertension. ..
  4. Kevin C Slep; Fiscal Year: 2014
    ..A host of microtubule associated proteins regulate the polymer's dynamics both spatially and temporally...
  5. Role of msps and tacc during axon guidance
    LAURA A LOWERY; Fiscal Year: 2010
    ..Finally, the proteins studied here are also misregulated in certain cancers. Thus, the research proposed here is of broad biomedical significance. ..
  6. Microtubule Stability in Lung Endothelium
    CRISTHIAAN D OCHOA ARENAS; Fiscal Year: 2012
    ....
  7. PEROXYNITRITE DAMAGE TO MICROTUBULE PROTEINS
    LISA MARIE LANDINO; Fiscal Year: 2011
    ..Researchers must consider and assess the effects of reactive molecules on abundant brain proteins because it is very likely that many proteins are damaged as we age. ..
  8. Rick B Meeker; Fiscal Year: 2016
    ..These studies will provide insights into the cellular dysfunction associated with age- and HIV- associated loss of neurotrophin support and will determine the therapeutic potential of the novel p75 ligand, LM11A-31. ..
  9. Angela N Carter; Fiscal Year: 2014
    ..We will employ a series of biochemical, pharmacological, neurophysiological and behavioral assays for these studies. ..
  10. Gloria Lee; Fiscal Year: 2016
    ..Our data will aid in the identification of new targets for disease therapeutics and new biomarkers for tracking disease progress. ..
  11. Dileep Varma; Fiscal Year: 2014
    ..As a contributor, Kevin Slep will provide equipment and expertise with protein purification and biochemical assays. As a 2nd contributor, Arshad Desai will provide training and expertise with C. elegans genetics and functional assays. ..
  12. Lee Ligon; Fiscal Year: 2016
    ..of the microtubule filament by post-translational modification of tubulin subunits or decoration by microtubule associated proteins (MAPs) may provide navigational cues for molecular motors...
  13. Joseph E Italiano; Fiscal Year: 2016
    ....
  14. Modeling Microtubule Dynamics in Mitosis
    David J Odde; Fiscal Year: 2013
    ..Understanding MT dynamics and their regulation by microtubule associated proteins in mitosis will allow us to more rationally develop new cancer treatment strategies...
  15. Phong T Tran; Fiscal Year: 2015
    ....
  16. JOHN CHARLES WILLIAMS; Fiscal Year: 2014
    ..State-of-the-art solid-state NMR spectroscopy will be introduced as a novel technique to probe the intrinsically insoluble and non-crystalline assemblies of microtubules with their associated proteins. ..
  17. The Roles of Steel Factor in Germ Cell Behavior in the Mouse
    Christopher C Wylie; Fiscal Year: 2010
    ..We will test the hypothesis that signals from adjacent tissues activate expression of Steel factor in cells immediately around the germ cells at the gastrula stage. ..
  18. David Sept; Fiscal Year: 2015
    ..underlying dynamic instability, and provides important insight into the mechanisms by which drugs and microtubule associated proteins can regulate microtubule dynamics...
  19. NICHOLAS COWAN; Fiscal Year: 2016
    ..2. Defective microtubule dynamics and/or interactions with Microtubule Associated Proteins (MAPs) Disease causing mutations might compromise microtubule dynamics or interfere with interaction(..
  20. The Micromechanics of Central Spindle Organization
    SCOTT THOMAS FORTH; Fiscal Year: 2013
    ....
  21. DCX Domain Proteins and Microtubule Transport in Cerebral Cortical Development
    Christian R Schubert; Fiscal Year: 2011
    ....
  22. FMRP-mediated translation regulation in neuronal development
    YUE contact FENG; Fiscal Year: 2010
    ..abstract_text> ..
  23. ORGANIZATION OF THE MAMMALIAN MITOTIC SPINDLE
    Duane A Compton; Fiscal Year: 2012
    ..Data generated from this work will provide insight into mechanisms of aneuploidy in tumor cells and may reveal strategies for therapy of chromosomally unstable aneuploid tumors. ..
  24. STUDIES ON MICROTUBULE BINDING PROTEINS
    Ronald A Milligan; Fiscal Year: 2010
    ..Additional work on microtubule associated proteins has provided insights into the mechanisms of selective microtubule stabilization by these proteins...
  25. MELISSA KLEIN GARDNER; Fiscal Year: 2016
    ....
  26. Edward M Campbell; Fiscal Year: 2014
    ....
  27. Elias T Spiliotis; Fiscal Year: 2016
    ..g., inhibitory peptides), which can be used as an alternative to microtubule-targeting agents. ..
  28. Induction of Cell Cleavage by the Mitotic Spindle
    Robert Margolis; Fiscal Year: 2009
    ..Further, three microtubule associated proteins, PRC1, MKLP1 and Cyk-4, are necessary for organization of the midzone spindle...
  29. Mechanisms of Action of Anti-Mitotic Drugs
    Mary Jordan; Fiscal Year: 2007
    ..The results will elucidate mechanisms and predict possible clinical efficacy of combination therapies directed at modulating mitotic microtubule regulatory proteins in combination with taxanes and Vinca alkaloids. ..
  30. CALMODULIN DEPENDENT KINASE AND SYNAPTIC FUNCTION
    MARY VALLANO; Fiscal Year: 1992
    ....
  31. PROTEIN COMPONENTS OF THE MITOTIC SPINDLE
    Richard Vallee; Fiscal Year: 1991
    b>Microtubule associated proteins (MAPs) from sea urchin eggs will be characterized. Preliminary studies have yielded a new procedure for isolating microtubules from this source which involves the use of taxol to promote assembly...
  32. PATHOBIOLOGY OF TAU PROTEIN
    KENNETH KOSIK; Fiscal Year: 1999
    ..Saporin is toxic to cells only when it reaches the cytosol. ..
  33. Colloquium on the Cytoskeleton and Human Disease
    Leslie Wilson; Fiscal Year: 2001
    ..Drs. Wilson, Briand, Jordan, Hamel, Marvaldi, and Binder have the primary responsibility for the scientific program, and Drs. Briand, Wilson, Briand, Wilson, Braguer, Peyrot, and Rossignol are in charge of meeting arrangements. ..
  34. BASIS OF SYNAPTIC PATHOLOGY IN ALZHEIMERS DISEASE
    Eliezer Masliah; Fiscal Year: 2001
    ..These studies will help to better understand the mechanisms of synaptic pathology in AD and to develop potential neuroprotective agents that will operate by enhancing the GT activity. ..
  35. NEURONAL POLARITY
    KENNETH KOSIK; Fiscal Year: 2000
    ..Finally, they will study the effects of other actin associated proteins on MAP organizational properties in ABP expressing; melanoma cells and in gelsolin deficient neuronal cultures. ..
  36. TOMOGRAPHIC ANALYSIS OF CYTOKINESIS IN ARABIDOPSIS
    L Staehelin; Fiscal Year: 2003
    ..abstract_text> ..
  37. TOMOGRAPHIC ANALYSIS OF CYTOKINESIS IN ARABIDOPSIS
    L Staehelin; Fiscal Year: 2002
    ..abstract_text> ..
  38. CYTOSKELETAL PROTEINS & TROPHIC FACTORS IN AGING
    EDWIN GROLLMAN; Fiscal Year: 1991
    ..for Phase II is the study of the effect and the mechanism of effect of neurotrophic factors on microtubule associated proteins (MAPs) and on the cytoskeletal structure of the aging neuron...
  39. ALKYLATING AGENTS AND DRUG RESISTANCE
    Kenneth Tew; Fiscal Year: 1999
    ..adaptations, especially with respect to tubulin and actin isoforins and altered expression of various microtubule associated proteins (MAP's) will be enacted with a view to understanding factors which may govern mitotic traverse ..
  40. MECHANISMS OF OCCUPATIONAL NEUROPATHIES
    MOHAMED ABOU DONIA; Fiscal Year: 1999
    ....
  41. ENDOTHELIAL CELL CYTOSKELETON IN HYPEROXIA
    Susan Harding; Fiscal Year: 1992
    ..5) Characterize cytoplasmic microtubules, microtubule associated proteins and quantitate cytoskeletal tubulin polymer in normoxic and oxygen exposed cells...
  42. PROPERTIES OF DYNEIN ISOENZYMES
    Ian Gibbons; Fiscal Year: 2003
    ....
  43. MECHANISMS OF CYTOSKELETAL INJURY BY NI COMPOUNDS
    Ken Lin; Fiscal Year: 1990
    ....
  44. Examination of PTL-1 in mechanosensory neurons of Caenorhabditis elegans
    KATHLEEN SUSMAN; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  45. Prognostic Significance of Neuronal Differentiation of Cutaneous Melanoma
    Vijayasaradhi Setaluri; Fiscal Year: 2009
    ..However, there are no biological markers to reliably assess the aggressiveness of melanoma. The goal of this project is to assess the value of novel markers in predicting melanoma tumor aggressiveness. ..
  46. BIOCHEMISTRY AND PATHOBIOLOGY OF MAP TWO IN NEURONS
    KENNETH KOSIK; Fiscal Year: 1990
    ..MAP2 and tau cDNA probes will be used to demonstrate ribosomal topography and their possible contribution of the compartmentalization. Finally, we propose to initiate dynamic studies of possible roles for the MAPs in neuronal transport...
  47. Thrombin signaling and HIV dementia
    Katherine Conant; Fiscal Year: 2006
    ..abstract_text> ..
  48. MECHANISM AND CONTROL OF BRAIN MICROTUBULE DYNAMICS
    Leslie Wilson; Fiscal Year: 2009
    ....
  49. BRAIN MACROPHAGES AND REPRODUCTIVE AGING
    Frederick Naftolin; Fiscal Year: 2002
    ..We will assess production of reactive oxygen species in these cultures by in-situ chemiluminescence and histochemistry. ..
  50. Arsenic disruption of neuron development
    Douglas Currie; Fiscal Year: 2009
    ..function;(i) Rho GTPases which are major regulators of actin dynamics in developing neurons and (ii) microtubule associated proteins (MAPs) which regulate microtubule stability...
  51. MAP1A AND MAP1B--SYNTHESIS, STRUCTURE/FUNCTION
    JAMES HAMMARBACK; Fiscal Year: 2000
    ..The last two aims directly test the role of MAP1 proteins in establishing, organizing and maintaining the axonal cytoskeleton. ..
  52. ESTRAMUSTINE AS AN ANTIMICROTUBULE AGENT IN THE PROSTATE
    Kenneth Tew; Fiscal Year: 1993
    ..effects in a diverse range of cells, including prostatic tumor cells, through binding to microtubule associated proteins (MAPs)...
  53. NEURONAL CYTOSKELETAL REMODELLING
    PETER MORIN; Fiscal Year: 1999
    ..We have set-up several vehicles via which I may obtain additional laboratory training and continue spending a small proportion of my time with clinically related matters. ..
  54. CSF MAP-2 IN TRAUMATIC BRAIN INJURY
    Frank Zemlan; Fiscal Year: 1999
    ..PROPOSED COMMERCIAL APPLICATION: The purpose of this Phase I application is to develop an in vitro test for identifying neuronal injury associated with traumatic brain damage. ..
  55. DEPRESSION, 5-HT1A RECEPTOR & NEUROPLASTICITY
    Efrain Azmitia; Fiscal Year: 2005
    ..The applicant will focus on dendritic collapse and apoptosis of neurons. This work will continue our long-term research into the interactions between serotonin and adult neumplasticity. ..
  56. CYTOSKELETAL INJURY & MECHANISM OF HERBICIDE TOXICITY
    Iih Nan Chou; Fiscal Year: 1991
    ..injuries due to alterations in the molecular species, phosphorylation state and distribution of microtubule associated proteins (MAPs) and their isoelectric variants? b...
  57. CALPAIN AND SUBSTRATES IN AGED AND ALZEHEIMER BRAINS
    LYNN PERLMUTTER; Fiscal Year: 1991
    ....
  58. GENETIC ANALYSIS OF TUBULIN AND INTERACTING PROTEINS
    Margaret Fuller; Fiscal Year: 1991
    ..of the tubulin molecule, and may be mediated by interactions between tubulin and different sets of microtubule associated proteins. Our long term goal is to elucidate both the cis and trans-acting mechanisms that regulate the ..
  59. DYNAMIC PROPERTIES OF THE CYTOSKELETON
    Michael Caplow; Fiscal Year: 1990
    ..We wish to further develop a kinetic model which we have recently derived for microtubule assembly and disassembly, which takes into account the GTP hydrolysis reaction...
  60. CYTOSKELETAL DYNAMICS DURING CELL SHAPE CHANGES
    JOHN HENSON; Fiscal Year: 1992
    ..state of these two major cytoskeletal elements is in turn regulated by a host of actin binding and microtubule associated proteins (MAP)...
  61. EXTRACELLULAR MATRIX MODULATION OF LYMPHOCYTE MOTILITY
    Stuart Ratner; Fiscal Year: 1999
    ..We will determine the effects of constitutively active and dominant negative mutant GTPases on integrin-induced appendage formation, adhesion, and motility. ..
  62. REGULATION OF MAP2 IN BRAIN
    Itzhak Fischer; Fiscal Year: 1991
    ....
  63. ALZHEIMER NEUROFIBRILLARY DEGENERATION
    Khalid Iqbal; Fiscal Year: 2001
    ..the phosphorylation of normal tau by which converts it to an AD-like state in sequestering normal microtubule associated proteins (MAP), MAP1 and MAP2 and depolymerizing microtubles...