metallothionein

Summary

Summary: A low-molecular-weight (approx. 10 kD) protein occurring in the cytoplasm of kidney cortex and liver. It is rich in cysteinyl residues and contains no aromatic amino acids. Metallothionein shows high affinity for bivalent heavy metals.

Top Publications

  1. pmc Metallothionein protection of cadmium toxicity
    Curtis D Klaassen
    Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160 7417, USA
    Toxicol Appl Pharmacol 238:215-20. 2009
  2. pmc Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors
    Hatice Ozer
    Department of Pathology, Cumhuriyet University, Faculty of Medicine, Sivas, 58140, Turkey
    Diagn Pathol 7:124. 2012
  3. ncbi Clinical utility of copper, ceruloplasmin, and metallothionein plasma determinations in human neurodegenerative patients and their first-degree relatives
    Nathalie Arnal
    INIBIOLP Instituto de Investigac iones Bioquímicas de La Plata, CCT La Plata, CONICET UNLP, Catedra de Bioquimica y Biologia Molecular, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, 60 y 120 1900 La Plata, Argentina
    Brain Res 1319:118-30. 2010
  4. ncbi Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis
    Christopher Cobbett
    Department of Genetics, University of Melbourne, Parkville, Australia 3052
    Annu Rev Plant Biol 53:159-82. 2002
  5. ncbi Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy
    Lu Cai
    Department of Medicine, The University of Louisville, Louisville, Kentucky, USA
    J Am Coll Cardiol 48:1688-97. 2006
  6. pmc A review of metallothionein isoforms and their role in pathophysiology
    N Thirumoorthy
    Dept, of Pharmaceutics, Cherraan s College of Pharmacy, Coimbatore 39, TN, India
    World J Surg Oncol 9:54. 2011
  7. ncbi Metallothionein-2A overexpression increases the expression of matrix metalloproteinase-9 and invasion of breast cancer cells
    Hyung Gyun Kim
    Department of Toxicology, College of Pharmacy, Chungnam National University, Yuseong Gu, Daejeon, South Korea
    FEBS Lett 585:421-8. 2011
  8. ncbi Metallothioneins I and II: neuroprotective significance during CNS pathology
    Roger K Stankovic
    Department of Pathology, University of Sydney, NSW, Australia
    Int J Biochem Cell Biol 39:484-9. 2007
  9. ncbi Zinc supplementation attenuates metallothionein and oxidative stress changes in kidney of streptozotocin-induced diabetic rats
    Dervis Ozcelik
    Departments of Biophysics, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
    Biol Trace Elem Res 150:342-9. 2012
  10. ncbi Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers
    J Gumulec
    Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    Neoplasma 59:191-201. 2012

Detail Information

Publications359 found, 100 shown here

  1. pmc Metallothionein protection of cadmium toxicity
    Curtis D Klaassen
    Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160 7417, USA
    Toxicol Appl Pharmacol 238:215-20. 2009
    ..horse kidney in 1957 marked the birth of research on this low-molecular weight, cysteine-rich protein called metallothionein (MT) in Cd toxicology...
  2. pmc Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors
    Hatice Ozer
    Department of Pathology, Cumhuriyet University, Faculty of Medicine, Sivas, 58140, Turkey
    Diagn Pathol 7:124. 2012
    ..evaluate immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, and bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in typing of benign, borderline, and malignant serous and mucinous ovarian tumors.
  3. ncbi Clinical utility of copper, ceruloplasmin, and metallothionein plasma determinations in human neurodegenerative patients and their first-degree relatives
    Nathalie Arnal
    INIBIOLP Instituto de Investigac iones Bioquímicas de La Plata, CCT La Plata, CONICET UNLP, Catedra de Bioquimica y Biologia Molecular, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, 60 y 120 1900 La Plata, Argentina
    Brain Res 1319:118-30. 2010
    ..Copper measurement and the Cu/CRP ratio may be predictive markers of risk for the first-degree relatives of AD patients. We believe that these results are valuable as a reliable clinical tool...
  4. ncbi Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis
    Christopher Cobbett
    Department of Genetics, University of Melbourne, Parkville, Australia 3052
    Annu Rev Plant Biol 53:159-82. 2002
    ..Recent advances in understanding the regulation of PC biosynthesis and MT gene expression and the possible roles of PCs and MTs in heavy metal detoxification and homeostasis are reviewed...
  5. ncbi Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy
    Lu Cai
    Department of Medicine, The University of Louisville, Louisville, Kentucky, USA
    J Am Coll Cardiol 48:1688-97. 2006
    ..We aimed to test whether attenuation of early-phase cardiac cell death can prevent diabetic cardiomyopathy...
  6. pmc A review of metallothionein isoforms and their role in pathophysiology
    N Thirumoorthy
    Dept, of Pharmaceutics, Cherraan s College of Pharmacy, Coimbatore 39, TN, India
    World J Surg Oncol 9:54. 2011
    The Metallothionein (MT) is a protein which has several interesting biological effects and has been demonstrated increase focus on the role of MT in various biological systems in the past three decades...
  7. ncbi Metallothionein-2A overexpression increases the expression of matrix metalloproteinase-9 and invasion of breast cancer cells
    Hyung Gyun Kim
    Department of Toxicology, College of Pharmacy, Chungnam National University, Yuseong Gu, Daejeon, South Korea
    FEBS Lett 585:421-8. 2011
    The overexpression of metallothionein-2A (MT-2A) is frequently observed in invasive human breast tumors and has been linked with more aggressive breast cancers...
  8. ncbi Metallothioneins I and II: neuroprotective significance during CNS pathology
    Roger K Stankovic
    Department of Pathology, University of Sydney, NSW, Australia
    Int J Biochem Cell Biol 39:484-9. 2007
    ..Hence, MT-I, II may provide neurotherapeutic targets offering protection against neuronal injury and degeneration...
  9. ncbi Zinc supplementation attenuates metallothionein and oxidative stress changes in kidney of streptozotocin-induced diabetic rats
    Dervis Ozcelik
    Departments of Biophysics, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
    Biol Trace Elem Res 150:342-9. 2012
    Zinc is an element that under physiological conditions preferentially binds to and is a potent inducer of metallothionein under physiological conditions...
  10. ncbi Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers
    J Gumulec
    Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    Neoplasma 59:191-201. 2012
    ..Alpha-methylacyl-CoA racemase (AMACR), metallothionein classes 1A and 2A (MT1A and MT2A) were determined and compared to prostate specific antigen (PSA) levels...
  11. ncbi Mammalian metallothionein in toxicology, cancer, and cancer chemotherapy
    Mohammad Namdarghanbari
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, Milwaukee, USA
    J Biol Inorg Chem 16:1087-101. 2011
    The present paper centers on mammalian metallothionein 1 and 2 in relationship to cell and tissue injury beginning with its reaction with Cd²⁺ and then considering its role in the toxicology and chemotherapy of both metals and non-..
  12. ncbi Divergent consequences arise from metallothionein overexpression in astrocytes: zinc buffering and oxidant-induced zinc release
    Latha M Malaiyandi
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Glia 45:346-53. 2004
    ..In this study, we overexpressed the zinc-binding protein metallothionein-II (MT) in astrocytes to assess its impact as (1) an acute zinc buffering mechanism, and (2) an oxidant-..
  13. ncbi MiR-23a in amplified 19p13.13 loci targets metallothionein 2A and promotes growth in gastric cancer cells
    Juan An
    Laboratory of Molecular Oncology, Key Laboratory of Carcinogenesis and Translational Research Ministry of Education, Peking University Cancer Hospital Institute, Beijing, 100142, P R, China
    J Cell Biochem 114:2160-9. 2013
    ..of mRNA profile and miRNA TargetScan predictions, we found that upregulation of miR-23a and downregulation of metallothionein 2A (MT2A) were detected simultaneously in 70% (7/10) of the miRNA and mRNA profiles...
  14. pmc Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice
    Hann Ling Wong
    Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Takayama 8916 5, Ikoma, Nara 630 0101, Japan
    Plant Physiol 135:1447-56. 2004
    ..We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors...
  15. pmc A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity
    C A Blindauer
    Department of Chemistry, University of Edinburgh, United Kingdom
    Proc Natl Acad Sci U S A 98:9593-8. 2001
    ..The inorganic core strongly resembles the Zn(4)Cys(11) cluster of mammalian metallothionein, despite different amino acid sequences, a different linear order of the ligands, and presence of histidine ..
  16. ncbi Role of metallothionein in cadmium traffic and toxicity in kidneys and other mammalian organs
    Ivan Sabolic
    Unit of Molecular Toxicology, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, Zagreb, Croatia
    Biometals 23:897-926. 2010
    ..Liberated Cd can immediately affect the cell structures and functions. The resulting proteinuria and CdMT in the urine can be used as biomarkers of tubular injury...
  17. ncbi Metallothioneins in human tumors and potential roles in carcinogenesis
    M George Cherian
    Department of Pathology, University of Western Ontario, London, Ont, Canada N6A 5C1
    Mutat Res 533:201-9. 2003
    ..Therefore, additional experimental data on MT and its isoforms in human tumors, are needed to elucidate the biological functions of MT during tumor progression, along with other tumor markers...
  18. ncbi Detection of metallothionein 1G as a methylated tumor suppressor gene in human hepatocellular carcinoma using a novel method of double combination array analysis
    Mitsuro Kanda
    Department of Surgery II, Graduate School and Faculty of Medicine, University of Nagoya, Showa Ku, Nagoya, Japan
    Int J Oncol 35:477-83. 2009
    ..b>Metallothionein (MT) 1G gene located on 16q13 showed a decreased expression in tumor tissue...
  19. ncbi Visualization of proteins in intact cells with a clonable tag for electron microscopy
    Elia Diestra
    Cell Structure Lab, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Darwin 3, Campus de Cantoblanco, Madrid 28049, Spain
    J Struct Biol 165:157-68. 2009
    ..To test the viability of the metal-binding protein metallothionein (MT) as a tag for TEM in cells we have studied three MT-fusion proteins in Escherichia coli: AmiC, a ..
  20. ncbi Roles of the metallothionein family of proteins in the central nervous system
    J Hidalgo
    Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain
    Brain Res Bull 55:133-45. 2001
    ..The focus of this review concerns the biology of the MT family in the context of their expression and functional roles in the central nervous system...
  21. ncbi Inhibition of superoxide generation and associated nitrosative damage is involved in metallothionein prevention of diabetic cardiomyopathy
    Lu Cai
    Department of Medicine, University of Louisville School of Medicine, 511 South Floyd St, MDR 533, Louisville, KY 40202, USA
    Diabetes 54:1829-37. 2005
    The mechanisms of metallothionein prevention of diabetic cardiomyopathy are largely unknown...
  22. pmc Metallothionein alleviates oxidative stress-induced endoplasmic reticulum stress and myocardial dysfunction
    Rui Guo
    Department of Biochemistry and Molecular Biology, Hebei University College of Life Sciences, Baoding 071002, China
    J Mol Cell Cardiol 47:228-37. 2009
    ..ER stress and contractile function in the absence or presence of the heavy metal scavenger antioxidant metallothionein (MT)...
  23. ncbi Regulation of metallothionein gene expression by oxidative stress and metal ions
    G K Andrews
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160 7421, USA
    Biochem Pharmacol 59:95-104. 2000
    ..Thus, regulation of the mouse MT-I gene by metals and oxidative stress involves multiple signaling pathways which depend on the species of metal ion and the nature of the oxidative stress...
  24. ncbi The novel metallothionein genes of Caenorhabditis elegans. Structural organization and inducible, cell-specific expression
    J H Freedman
    Department of Molecular Pharmacology, Atran Laboratories, Albert Einstein College of Medicine, Bronx, New York 10461
    J Biol Chem 268:2554-64. 1993
    ..The inducibility and cell type specificity of metallothionein gene expression were investigated in transgenic C...
  25. pmc Inactivation of GSK-3beta by metallothionein prevents diabetes-related changes in cardiac energy metabolism, inflammation, nitrosative damage, and remodeling
    Yuehui Wang
    Chinese American Research Institute for Diabetic Complications, Wenzhou Medical College, Zhejiang, China
    Diabetes 58:1391-402. 2009
    Glycogen synthase kinase (GSK)-3beta plays an important role in cardiomyopathies. Cardiac-specific metallothionein-overexpressing transgenic (MT-TG) mice were highly resistant to diabetes-induced cardiomyopathy...
  26. pmc Suppression of metallothionein-I/II expression and its probable molecular mechanisms
    Samson T Jacob
    Department of Molecular and Cellular Biochemistry, College of Medicine, The Ohio State University, 333 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    Environ Health Perspect 110:827-30. 2002
    b>Metallothionein (MT) promoter was methylated in rat hepatoma and in mouse lymphosarcoma cells by methylation of cytosine within the CpG dinucleotide region...
  27. pmc Molecular functions of metallothionein and its role in hematological malignancies
    Shinichiro Takahashi
    Division of Hematology, Kitasato University School of Allied Health Sciences, 1 15 1 Kitasato, Minami Ku, Sagamihara, 252 0373, Japan
    J Hematol Oncol 5:41. 2012
    b>Metallothionein (MT) was reported to be a potential negative regulator of apoptosis, and various reports have suggested that it may play roles in carcinogenesis and drug resistance, in at least a portion of cancer cells...
  28. ncbi Metallothioneins 1 and 2 attenuate peroxynitrite-induced oxidative stress in Parkinson disease
    Manuchair Ebadi
    Department of Pharmacology, University of North Dakota School of Medicine and Health Sciences, 501 North Columbia Road, Grand Forks, ND 58203, USA
    Exp Biol Med (Maywood) 231:1576-83. 2006
    ..generator 3-morpholinosydnonimine (SIN-1)-induced neurotoxicity in control wild-type (control(wt)) mice, metallothionein double knockout (MT(dko)) mice, metallothionein-transgenic (MT(trans)) mice, and in cultured human ..
  29. ncbi Characterization of MtnE, the fifth metallothionein member in Drosophila
    Lilit Atanesyan
    Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
    J Biol Inorg Chem 16:1047-56. 2011
    ..Here we characterize a new fifth member of Drosophila MT gene family, coding for metallothionein E (MtnE). The MtnE transcription unit is located head-to-head with the one of MtnD...
  30. ncbi Early-phase immunodetection of metallothionein and heat shock proteins in extruded earthworm coelomocytes after dermal exposure to metal ions
    Joanna Homa
    Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, R Ingardena 6, PL 30 060 Krakow, Poland
    Environ Pollut 135:275-80. 2005
    ..bio-reactive quantities of metals within 3 days after dermal exposure, and that they respond by upregulating metallothionein (MT) and heat shock protein (HSP70, HSP72) expression...
  31. ncbi Metallothioneins and cancer
    Tomas Eckschlager
    Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno CZ 613 00, Czech Republic
    Curr Protein Pept Sci 10:360-75. 2009
    ..The role of MTs as a tumor disease marker or as a cause of resistance in cancer treatment is reviewed and discussed. Moreover, we describe some analytical methods that were developed to detect MTs...
  32. pmc Metallothionein abrogates GTP cyclohydrolase I inhibition-induced cardiac contractile and morphological defects: role of mitochondrial biogenesis
    Asli F Ceylan-Isik
    Center for Cardiovascular Research and Alternative Medicine, University of Wyoming College of Health Sciences, Laramie, WY 82071, USA
    Hypertension 53:1023-31. 2009
    ..designed to examine the effect of BH4 deficiency on cardiac morphology and function, as well as the impact of metallothionein (MT) on BH4 deficiency-induced abnormalities, if any...
  33. ncbi Interaction of metallothionein with tumor suppressor p53 protein
    Elena A Ostrakhovitch
    Department of Pathology, University of Western Ontario, London, Ont, Canada N6A 5C1
    FEBS Lett 580:1235-8. 2006
    Previous reports have shown that metallothionein (MT) may modulate p53 activity through zinc exchange. However, little is known on a direct interaction between MT and p53 in cells...
  34. ncbi The "magic numbers" of metallothionein
    Duncan E K Sutherland
    Department of Chemistry, The University of Western Ontario, London, ON, Canada
    Metallomics 3:444-63. 2011
    ..The most well studied member of the family is the mammalian metallothionein, which consists of two domains: a β-domain with 9 cysteine residues, which sequesters 3 Cd(2+) or Zn(2+) or ..
  35. ncbi Sub-cellular partitioning of cadmium, copper, nickel and zinc in indigenous yellow perch (Perca flavescens) sampled along a polymetallic gradient
    Anik Giguere
    INRS ETE, Universite du Quebec, Quebec City, Canada
    Aquat Toxicol 77:178-89. 2006
    ..among potentially metal-sensitive fractions (cytosolic enzymes, organelles) and detoxified metal fractions (metallothionein) was determined after differential centrifugation of fish liver homogenates...
  36. ncbi Isoflavonoid photoprotection in mouse and human skin is dependent on metallothionein
    Sitarina Widyarini
    Faculty of Veterinary Science, University of Sydney, New South Wales, Australia
    J Invest Dermatol 126:198-204. 2006
    ..might result from their interaction with one cutaneous antioxidant known to modulate UV photodamage, metallothionein (MT)...
  37. ncbi Metallothionein 3: an androgen-upregulated gene enhances cell invasion and tumorigenesis of prostate carcinoma cells
    Horng Heng Juang
    Department of Anatomy, College of Medicine, Chang Gung University, Kwei Shan, Tao Yuan, Taiwan
    Prostate 73:1495-506. 2013
    ..We determined the effects of androgen, cadmium, and arsenic on MT1/2 and MT3 in prostate carcinoma cells, and evaluated the functional effects of MT3 on cell proliferation, invasion, and tumorigenesis...
  38. ncbi Evidence that a copper-metallothionein complex is responsible for fluorescence in acid-secreting cells of the Drosophila stomach
    M McNulty
    Department of Neurobiology, Pharmacology, and Physiology, University of Chicago, IL 60637, USA
    Cell Tissue Res 304:383-9. 2001
    ..observations that (1) a similar fluorescent signal in yeast is produced by a complex between copper and metallothionein, and (2) metallothionein is expressed constitutively in the copper cell region and inducibly in other regions ..
  39. ncbi The potential effect of metallothionein 2A -5A/G single nucleotide polymorphism on blood cadmium, lead, zinc and copper levels
    Zeliha Kayaalti
    Ankara University Institute of Forensic Sciences Dikimevi, 06590 Ankara, Turkey
    Toxicol Appl Pharmacol 256:1-7. 2011
    ..The aim of this study was to investigate the association between the metallothionein 2A (MT2A) core promoter region -5 A/G single nucleotide polymorphism (SNP) and Cd, Pb, Zn and Cu levels in ..
  40. pmc Gains, losses and changes of function after gene duplication: study of the metallothionein family
    Ana Moleirinho
    IPATIMUP Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
    PLoS ONE 6:e18487. 2011
    ..Altogether our findings provide novel data on the evolution and diversification of MT gene duplicates, a valuable resource for understanding the vast set of biological processes in which these proteins are involved...
  41. ncbi Metallothionein alleviates glutathione depletion-induced oxidative cardiomyopathy in murine hearts
    Jun Ren
    Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY, USA
    Crit Care Med 36:2106-16. 2008
    ..This study was designed to examine the impact of glutathione depletion and the free radical scavenger, metallothionein (MT), on cardiac function.
  42. ncbi Zinc: a multipurpose trace element
    M Stefanidou
    Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75, Mikras Asias Street, Goudi, Athens 11527, Greece
    Arch Toxicol 80:1-9. 2006
    ..Zinc is not stored in the body and excess intakes result in reduced absorption and increased excretion. Nevertheless, there are cases of acute and chronic Zn poisoning...
  43. ncbi Protective effect of metallothionein on oxidative stress-induced DNA damage
    Natalie Chiaverini
    Laboratory for Biochemistry, Department of Chemistry, Katholieke Universiteit Leuven, BE 3001 Heverlee, Belgium
    Free Radic Res 44:605-13. 2010
    ..In this review, the involvement of MT in the protection of DNA against oxidative stress is discussed...
  44. ncbi Crystal structure of the cyanobacterial metallothionein repressor SmtB: a model for metalloregulatory proteins
    W J Cook
    Department of Pathology, University of Alabama at Birmingham 35294, USA
    J Mol Biol 275:337-46. 1998
    ..PCC7942 is a trans-acting dimeric repressor that is required for Zn(2+)-responsive expression of the metallothionein SmtA. The structure of SmtB was solved using multiple isomorphous replacement techniques and refined at 2...
  45. ncbi The metabolomic responses of Caenorhabditis elegans to cadmium are largely independent of metallothionein status, but dominated by changes in cystathionine and phytochelatins
    Samantha L Hughes
    School of Biomedical and Health Sciences, Pharmaceutical Science Division, King s College London, Franklin Wilkins Building, Stamford Street, London SE1 9NH, United Kingdom
    J Proteome Res 8:3512-9. 2009
    ..Neither in the presence nor absence of cadmium did the metallothionein status (single or double mtl knockouts) markedly modulate the metabolic profile...
  46. ncbi Metallothionein expression in hepatocellular carcinoma
    Geng Wen Huang
    Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
    World J Gastroenterol 8:650-3. 2002
    ..To investigate the expression of metallothioneins (MTs), which were recently thought to have close relationship with tumors, in human hepatocellular carcinoma...
  47. ncbi Polymorphisms in metallothionein-1 and -2 genes associated with the risk of type 2 diabetes mellitus and its complications
    Lina Yang
    School of Public Health, Jilin University, Changchun, China
    Am J Physiol Endocrinol Metab 294:E987-92. 2008
    b>Metallothionein (MT) as a potent antioxidant can affect energy metabolism. The present study was undertaken to investigate the association between MT gene polymorphism and type 2 diabetes mellitus...
  48. ncbi Immunohistochemical metallothionein expression in hepatocellular carcinoma: relation to tumor progression and chemoresistance to platinum agents
    Tsunehiro Endo
    First Department of Internal Medicine, Chiba University School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba, 260 8670, Japan
    J Gastroenterol 39:1196-201. 2004
    b>Metallothionein (MT), which is known to detoxify heavy metal ions, is considered to serve as a mechanism of resistance to platinum complex compounds...
  49. ncbi Metallothionein protects bone marrow stromal cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation
    An Ling Liu
    Yingdong College of Biotechnology, Shaoguan University, Shaoguan, Guangdong Province 512005, PR China
    Cell Biol Int 28:905-11. 2004
    b>Metallothionein (MT), a cysteine-rich, metal-binding protein, is involved in homeostatic regulation of essential metals and protection of cells against oxidative injury...
  50. pmc Loss of metallothionein predisposes mice to diethylnitrosamine-induced hepatocarcinogenesis by activating NF-kappaB target genes
    Sarmila Majumder
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio, USA
    Cancer Res 70:10265-76. 2010
    ..Taken together, these results demonstrate that MTs protect mice from hepatocarcinogen-induced liver damage and carcinogenesis, underscoring their potential therapeutic application against hepatocellular cancer...
  51. ncbi Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae
    Giusy M Adamo
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan IT 20126, Italy
    Microbiology 158:2325-35. 2012
    ..The major difference detected in the two strains was the copy number of the gene CUP1, which encodes a metallothionein. In evolved cells, a sevenfold amplification of CUP1 was observed, accounting for its strongly and steadily ..
  52. pmc ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene
    D J Thiele
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109 0606
    Mol Cell Biol 8:2745-52. 1988
    ..ACE1 maps to the left arm of chromosome VII, 9 centimorgans centromere distal to lys5. The ACE1 gene appears to play a direct or indirect positive role in activation of CUP1 expression in response to elevated copper concentrations...
  53. ncbi Tissue-specific cadmium and metallothionein levels in freshwater crab Sinopotamon henanense during acute exposure to waterborne cadmium
    Wenli Ma
    School of Life Science and Technology, Shanxi University, Taiyuan 030006, People s Republic of China
    Environ Toxicol 23:393-400. 2008
    ..The relationship between tissue-specific Cd accumulation and metallothionein (MT) induction was investigated using the Cd saturation assay and atomic absorption spectrophotometry method...
  54. ncbi Photoperiod affects hepatic and renal cadmium accumulation, metallothionein induction, and cadmium toxicity in the wild bank vole (Clethrionomys glareolus)
    T Włostowski
    Institute of Biology, University of Białystok, Swierkowa 20B, Białystok 15 950, Poland
    Ecotoxicol Environ Saf 58:29-36. 2004
    ..Histological examinations and analyses of metallothionein (MT), Cd, Cd bound and not bound to MT, iron and lipid peroxidation in the liver and kidneys were carried out...
  55. ncbi Cadmium accumulation, metallothionein and glutathione levels, and histopathological changes in the kidneys and liver of magpie (Pica pica) from a zinc smelter area
    Tadeusz Włostowski
    Institute of Biology, University of Białystok, Swierkowa 20B, 15 950, Białystok, Poland
    Ecotoxicology 19:1066-73. 2010
    ..The concentrations of metallothionein (MT) and glutathione (GSH) that are linked to a protective effect against Cd toxicity were also determined...
  56. ncbi Metallothionein is a potential negative regulator of apoptosis
    Ryuya Shimoda
    National Cancer Institute at the National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 73:294-300. 2003
    ..Previous studies have revealed that metallothionein (MT) can protect cells from apoptosis induced by oxidative stress and metals...
  57. ncbi Isolation of a prokaryotic metallothionein locus and analysis of transcriptional control by trace metal ions
    J W Huckle
    Department of Biological Sciences, University of Durham, UK
    Mol Microbiol 7:177-87. 1993
    ....
  58. pmc Glucose regulates free cytosolic Zn²⁺ concentration, Slc39 (ZiP), and metallothionein gene expression in primary pancreatic islet β-cells
    Elisa A Bellomo
    Section of Cell Biology, Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK
    J Biol Chem 286:25778-89. 2011
    ..expression of the Zn²⁺ importer family members Slc39a6, Slc39a7, and Slc39a8, and decreased expression of metallothionein 1 and 2...
  59. ncbi Metallothionein is a crucial protective factor against Helicobacter pylori-induced gastric erosive lesions in a mouse model
    Masaharu Mita
    Laboratory Animal Research Center, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    Am J Physiol Gastrointest Liver Physiol 294:G877-84. 2008
    ..b>Metallothionein (MT) is a low-molecular-weight, cysteine-rich protein that can act not only as a metal-binding protein, but ..
  60. ncbi Expression of metallothionein and p53 antigens are correlated in oral squamous cell carcinoma
    Sergio Vitorino Cardoso
    Department of Oral Pathology, Federal University of Minas Gerais, Av Antonio Carlos, 6627, Belo Horizonte, Brazil
    Anticancer Res 29:1189-93. 2009
    b>Metallothionein and p53 proteins have been associated with tumoral evolution and resistance against therapy. Experimentally, the former may modulate the activity of tumor suppressor protein through zinc exchange...
  61. ncbi Metallothionein redox cycle and function
    Y James Kang
    Department of Medicine, University of Louisville School of Medicine, 511 S Floyd Street, MDR 530, Louisville, KY 40202, USA
    Exp Biol Med (Maywood) 231:1459-67. 2006
    The biologic function of metallothionein (MT) has been a perplexing topic ever since the discovery of this protein...
  62. ncbi Metallothionein-null mice are more sensitive than wild-type mice to liver injury induced by repeated exposure to cadmium
    S S Habeebu
    Center for Environmental and Occupational Health, Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas 66160 7417, USA
    Toxicol Sci 55:223-32. 2000
    Liver is a major target organ of cadmium (Cd) toxicity following acute and chronic exposure. Metallothionein (MT), a low-molecular-weight, cysteine-rich, metal-binding protein has been shown to play an important role in protection ..
  63. ncbi Prior increase in metallothionein levels is required to prevent doxorubicin cardiotoxicity
    Xiuhua Sun
    Department of Medicine, University of Louisville School of Medicine, and Jewish Hospital Heart and Lung Institute, Louisville, Kentucky 40202, USA
    Exp Biol Med (Maywood) 227:652-7. 2002
    Many studies have shown that metallothionein (MT) can be increased significantly by different oxidative insults in multiple organ systems. However, the increase in MT production often fails to protect against oxidative tissue injury...
  64. ncbi Downregulation of metallothionein 1F, a putative oncosuppressor, by loss of heterozygosity in colon cancer tissue
    Dong wang Yan
    Department of General Surgery, Shanghai Jiaotong University Affiliated First People s Hospital, 85 Wujin Road, People s Republic of China
    Biochim Biophys Acta 1822:918-26. 2012
    Downregulation of metallothionein (MT) genes has been reported in several tumors with discrepant results...
  65. pmc Evidence for a protective role of metallothionein-1 in focal cerebral ischemia
    M van Lookeren Campagne
    Department of Cardiovascular Research, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 96:12870-5. 1999
    ..These results provide evidence that increased expression of MT-1 protects against focal cerebral ischemia and reperfusion...
  66. pmc Metallothionein - overexpression as a highly significant prognostic factor in melanoma: a prospective study on 1270 patients
    G Weinlich
    Clinical Department of Dermatology and Venerology, Innsbruck Medical University, Anichstrasse 35, Innsbruck A 6020, Austria
    Br J Cancer 94:835-41. 2006
    ..1; 95% CI 4.7-10.9; P<0.0001 for survival), as well as multivariate analysis with other prognostic markers resulted in MT overexpression as a highly significant and independent factor for prognosis in primary melanoma...
  67. ncbi Protective effects of metallothionein against dopamine quinone-induced dopaminergic neurotoxicity
    Ikuko Miyazaki
    Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2 5 1 Shikatacho, Okayama 700 8558, Japan
    FEBS Lett 581:5003-8. 2007
    ..Here, we examined the effects of cysteine-rich metal-binding proteins, metallothionein (MT)-1 and -2, on DA quinone-induced neurotoxicity. MT quenched DA semiquinones in vitro...
  68. ncbi Mammalian zinc transport, trafficking, and signals
    Robert J Cousins
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611 0370, USA
    J Biol Chem 281:24085-9. 2006
  69. ncbi Metallothionein-I+II in neuroprotection
    Mie Ø Pedersen
    Section of Neuroprotection, Institute of Neuroscience and Pharmacology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Biofactors 35:315-25. 2009
    b>Metallothionein (MT)-I+II synthesis is induced in the central nervous system (CNS) in response to practically any pathogen or disorder, where it is increased mainly in reactive glia...
  70. ncbi Metallothioneins: unparalleled diversity in structures and functions for metal ion homeostasis and more
    Claudia A Blindauer
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    Nat Prod Rep 27:720-41. 2010
    ....
  71. ncbi Metallothionein prevents high-fat diet induced cardiac contractile dysfunction: role of peroxisome proliferator activated receptor gamma coactivator 1alpha and mitochondrial biogenesis
    Feng Dong
    Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, WY 82071, USA
    Diabetes 56:2201-12. 2007
    ..This study was designed to evaluate the impact of the free radical scavenger metallothionein on high-fat diet-induced myocardial, intracellular Ca(2+), and mitochondrial dysfunction...
  72. ncbi Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers
    J C Amiard
    Universite de Nantes, Pôle Mer et Littoval, SMAB EA 2160, Service d Ecotoxicologie, Nantes, France
    Aquat Toxicol 76:160-202. 2006
    The literature on metallothioneins (MT) and metallothionein-like proteins (MTLP) in aquatic invertebrates is large and increasing, and yet inconsistencies and confusion remain, not least over the physiological role of MT and their use as ..
  73. ncbi Metallothionein: the multipurpose protein
    P Coyle
    Division of Clinical Biochemistry, Institute of Medical and Veterinary Science, Adelaide, SA
    Cell Mol Life Sci 59:627-47. 2002
    ..Use of mice with altered gene expression has enhance our understanding of the multifaceted role of MT, emphasised in this review...
  74. pmc Identification of a copper-binding metallothionein in pathogenic mycobacteria
    Ben Gold
    Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, New York 10065, USA
    Nat Chem Biol 4:609-16. 2008
    ..We propose that the product of the MT0196 gene be named mycobacterial metallothionein (MymT)...
  75. ncbi Zinc-buffering capacity of a eukaryotic cell at physiological pZn
    Artur Krezel
    Division of Human Nutrition, Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, TX 77555, USA
    J Biol Inorg Chem 11:1049-62. 2006
    ..Jointly, metallothionein and thionein function as the major zinc buffer under conditions of increased cellular zinc.
  76. pmc Targeted disruption of metallothionein I and II genes increases sensitivity to cadmium
    B A Masters
    Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104
    Proc Natl Acad Sci U S A 91:584-8. 1994
    We inactivated the mouse metallothionein (MT)-I and MT-II genes in embryonic stem cells and generated mice homozygous for these mutant alleles. These mice were viable and reproduced normally when reared under normal laboratory conditions...
  77. ncbi Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation
    Francesco Dondero
    University of Piemonte Orientale Amedeo Avogadro, Department of Environmental and Life Sciences, Molecular Physiology Unit, Via Trotti 17, 15100, Alessandria, Italy
    Gene 345:259-70. 2005
    The mRNA levels of two components of the mussel (Mytilus galloprovincialis) metallothionein (MT) gene families, MT10 and MT20, were evaluated using real-time quantitative-PCR and Sybr Green I chemistry in animals exposed to heavy metals ..
  78. ncbi The possible biological role of metallothionein in apoptosis
    Magdalena Dutsch-Wicherek
    Department of Otolaryngology, Head and Neck Surgery of the Jagiellonian University, Krakow, Poland
    Front Biosci 13:4029-38. 2008
    Metallothioneins (MTs) are a family of low molecular weight proteins with a high affinity for divalent metals. Metallothionein has been shown to have a protective role in apoptosis. Specifically, it controls the cellular zinc ion levels...
  79. ncbi Metallothionein suppresses angiotensin II-induced nicotinamide adenine dinucleotide phosphate oxidase activation, nitrosative stress, apoptosis, and pathological remodeling in the diabetic heart
    Guihua Zhou
    Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky, USA
    J Am Coll Cardiol 52:655-66. 2008
    We evaluated metallothionein (MT)-mediated cardioprotection from angiotensin II (Ang II)-induced pathologic remodeling with and without underlying diabetes.
  80. ncbi Redox biochemistry of mammalian metallothioneins
    Wolfgang Maret
    King s College London, Metal Metabolism Group, Diabetes and Nutritional Sciences Division, School of Medicine, London UK
    J Biol Inorg Chem 16:1079-86. 2011
    b>Metallothionein (MT) is a generic name for certain families of structurally rather variable metal-binding proteins...
  81. ncbi Zinc coordination environments in proteins as redox sensors and signal transducers
    Wolfgang Maret
    Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, 77555, USA
    Antioxid Redox Signal 8:1419-41. 2006
    ..The paradigm of such redox transducers is the zinc protein metallothionein, which, together with its apoprotein, thionein, functions at a central node in cellular signaling by ..
  82. ncbi Overlapping gene expression profiles of cell migration and tumor invasion in human bladder cancer identify metallothionein 1E and nicotinamide N-methyltransferase as novel regulators of cell migration
    Y Wu
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    Oncogene 27:6679-89. 2008
    ..identified genes known to be associated with cell invasion such as versican, and novel ones, including metallothionein 1E (MT1E) and nicotinamide N-methyltransferase (NNMT), whose expression correlated positively with cancer ..
  83. ncbi Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays
    C Gross
    University of Utah Health Sciences Center, Departments of Medicine and Biochemistry, Salt Lake City, Utah 84132, USA
    J Biol Chem 275:32310-6. 2000
    ..Copper-induced FET3 and FTR1 expression arises from an indirect copper effect on cellular iron pools...
  84. ncbi Metallothionein expression in human neoplasia
    S E Theocharis
    Department of Tumour Biology, Institut Curie, Paris, France
    Histopathology 45:103-18. 2004
    The metallothionein family is a class of low-molecular-weight, cysteine-rich proteins with high affinity for metal ions...
  85. pmc Functional analysis of the transcription repressor PLU-1/JARID1B
    Angelo G Scibetta
    Breast Cancer Biology Group, King s College London School of Medicine, 3rd Floor, Thomas Guy House, Guy s Hospital, London SE1 9RT, United Kingdom
    Mol Cell Biol 27:7220-35. 2007
    ..Chromatin immunoprecipitation assays confirmed that the metallothionein 1H (MT1H), -1F, and -1X genes are direct transcriptional targets of PLU-1/JARID1B in vivo...
  86. ncbi Advances in metallothionein structure and functions
    Milan Vasak
    Institute of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Trace Elem Med Biol 19:13-7. 2005
    ..In contrast, the biological properties of the brain-specific MT-3 isoform imply that the clusters in this protein play a structural role. The recent highlights of MT research are the subject of this review...
  87. ncbi Antioxidant role of metallothioneins: a comparative overview
    A Viarengo
    Dipartimento di Scienze e Tecnologie Avanzate, Universita del Piemonte Orientale Amedeo Avogadro, Alessandria, Italy
    Cell Mol Biol (Noisy-le-grand) 46:407-17. 2000
    ..Finally, as the relevance of redox processes in cell signaling is becoming more and more evident, a search for MT effects on redox signaling could represent a turning point in the understanding of the functional role of these protein...
  88. pmc Regulation of zinc homeostasis by inducible NO synthase-derived NO: nuclear metallothionein translocation and intranuclear Zn2+ release
    Daniela U Spahl
    Institute of Molecular Medicine, Research Group Immunobiology, Medical Department, Heinrich Heine University, Universitatsstrasse 1, D 40225 Dusseldorf, Germany
    Proc Natl Acad Sci U S A 100:13952-7. 2003
    ..via NO donors resulting in nitrosative stress leads to cytoplasmic Zn2+ release from the zinc storing protein metallothionein (MT) and probably other proteins that complex Zn2+ via cysteine thiols...
  89. ncbi Longevity and heavy metal resistance in daf-2 and age-1 long-lived mutants of Caenorhabditis elegans
    D Barsyte
    The School of Biological Sciences, The University of Manchester, England
    FASEB J 15:627-34. 2001
    ..As metallothionein (MT) proteins play a major role in metal detoxification, we examined the expression of MT genes both under ..
  90. ncbi Correlation between expression of metallothionein and expression of Ki-67 and MCM-2 proliferation markers in non-small cell lung cancer
    Bozena Werynska
    Department of Pulmonology and Pulmonary Tumours, Medical University in Wroclaw, Wroclaw, Poland
    Anticancer Res 31:2833-9. 2011
    ..This study aimed at the evaluation of the intensity of metallothionein (MT-I/II) expression in various histological types of non-small cell lung cancer (NSCLC) and correlation of ..
  91. ncbi Independent metal-binding features of recombinant metallothioneins convergently draw a step gradation between Zn- and Cu-thioneins
    Roger Bofill
    Dept de Química, Fac de Ciències, Universitat Autonoma de Barcelona, Barcelona, Spain
    Metallomics 1:229-34. 2009
    ..Overall, the analyzed trends allow the proposal of a chemically- and biologically-sound new reflection on MT classification criteria...
  92. pmc Zn2+-sensing by the cyanobacterial metallothionein repressor SmtB: different motifs mediate metal-induced protein-DNA dissociation
    J S Turner
    Department of Biochemistry and Genetics, The Medical School, University of Newcastle, UK
    Nucleic Acids Res 24:3714-21. 1996
    ..of repressors which dissociate from DNA in the presence of metals; Zn2+ being the most potent inducer of metallothionein gene (smtA) transcription in vivo...
  93. ncbi Correlation between metallothionein (MT) expression and selected prognostic factors in ductal breast cancers
    Agnieszka Gomulkiewicz
    Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland
    Folia Histochem Cytobiol 48:242-8. 2010
    Our study aimed at examining significance of metallothionein (MT) expression in ductal breast cancers by determination of a relationship between expression of MT protein (MT-1/2) and selected prognostic factors, including grade of ..
  94. ncbi Metallothionein 1E is methylated in malignant melanoma and increases sensitivity to cisplatin-induced apoptosis
    William J Faller
    UCD School of Biomolecular and Biomedical Science, UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
    Melanoma Res 20:392-400. 2010
    ..Overall, these studies suggest that MT1E is a potential tumour suppressor gene, whose loss may promote resistance to apoptosis-inducing therapies...
  95. pmc Metallothionein as a clonable high-density marker for cryo-electron microscopy
    Cedric Bouchet-Marquis
    Dept of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, CO 80309 0347, USA
    J Struct Biol 177:119-27. 2012
    ..Here we report on the application of a small metal-clustering protein, metallothionein (MTH), as a clonable label capable of clustering metal atoms into a high-density particle with high spatial ..
  96. ncbi Induction of astrocyte metallothioneins (MTs) by zinc confers resistance against the acute cytotoxic effects of methylmercury on cell swelling, Na+ uptake, and K+ release
    M Aschner
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    Brain Res 813:254-61. 1998
    b>Metallothionein (MT) proteins play an important role in the detoxification of heavy metals...
  97. ncbi Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene
    M Karin
    Nature 308:513-9. 1984
    ..have defined two stretches of DNA (genetic elements), lying close to the promoter for a human gene for metallothionein, that separately mediate the induction of the gene by heavy metal ions, particularly cadmium, and by ..
  98. ncbi Correlation of metallothionein expression with clinical progression of cancer in the oral cavity
    Jolanta Szelachowska
    Departments of Oncology, Wroclaw Medical University, Poland
    Anticancer Res 29:589-95. 2009
    This study aimed at finding out whether the expression of metallothionein (MT), laminin, Ki-67 antigen and minichromosome maintenance-2 (Mcm-2) protein changes with growing invasiveness of the tumour...
  99. ncbi Metallothionein 1 is a downstream target of vascular endothelial zinc finger 1 (VEZF1) in endothelial cells and participates in the regulation of angiogenesis
    Hiroki Miyashita
    Department of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
    Endothelium 12:163-70. 2005
    ..previous microarray analysis revealed that the down-regulation of VEZF1 gene resulted in the decrease of metallothionein 1 (MT1) gene in endothelial cells...
  100. ncbi Effects of cadmium telluride quantum dots on cadmium bioaccumulation and metallothionein production to the freshwater mussel, Elliptio complanata
    Caroline Peyrot
    Department of Chemistry, Universite de Montreal, PO Box 6128 Downtown, Montreal, QC, Canada H3C 3J7
    Comp Biochem Physiol C Toxicol Pharmacol 150:246-51. 2009
    ..After the exposure period, Cd bioaccumulation in the gills, digestive gland and gonad tissues and metallothionein (MT) levels were determined...
  101. ncbi Nitric oxide-induced changes in intracellular zinc homeostasis are mediated by metallothionein/thionein
    Claudette M St Croix
    Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol Lung Cell Mol Physiol 282:L185-92. 2002
    We hypothesized that metallothionein (MT), a cysteine-rich protein with a strong affinity for Zn(2+), plays a role in nitric oxide (NO) signaling events via sequestration or release of Zn(2+) by the unique thiolate clusters of the ..

Research Grants62

  1. A Novel Murine Model of Chronic Inflammatory Periodontitus
    Helieh S Oz; Fiscal Year: 2010
    ..glutathione, GST, metallothionein) to establishing homeostasis in the periodontium...
  2. Molecular Mechanisms of Intestinal Metal Ion Transport During Iron Deficiency
    James F Collins; Fiscal Year: 2013
    ..In enterocytes, a copper transporting ATPase (Atp7a) and a copper-binding protein (metallothionein) were upregulated in the setting of increased intracellular copper levels...
  3. Cisplatin Resistance mediated by copper export pathways
    Stephen B Howell; Fiscal Year: 2013
    ..produce cisplatin (DDP) resistance when over-expressed, the others being another Cu exporter, ATP7A, and metallothionein II...
  4. Role of Metallothioneins in Isoflurane-Preconditioning of neurons in vitro.
    Adam C Hall; Fiscal Year: 2010
    ..Recent work from my lab identified metallothionein I + II genes (MT-I + II) as among those significantly regulated in rat liver, kidney and heart following a 2% ..
  5. Extracellular Zinc Buffering and A-beta Oligomerization
    GUIDO FAAS; Fiscal Year: 2012
    ..The same will be done to measure the Zn2+ binding dynamics of the Zn2+-binding protein metallothionein-3 (MT-3) which is enriched at Zn2+ releasing synapses...
  6. Gastric Injury In Trauma and Surgical Sepsis
    David I Soybel; Fiscal Year: 2010
    ..of various zinc transporters, vesicular storage sites, and metal binding proteins in the cytoplasm, of which metallothionein (MT) is a major reservoir...
  7. Homocysteine: Vascular Biochemistry and Metabolism
    DONALD WELDON JACOBSEN; Fiscal Year: 2010
    ..g., metallothionein) targeted by homocysteine and how loss of function of targeted proteins leads to vascular cell dysfunction...
  8. Critical Roles for Iron and Copper Detoxification in Borrelia burgdorferi
    Xin Li; Fiscal Year: 2013
    ..data show that (i) BicA is a novel fusion between an iron-binding ferritin-like molecule and a copper-binding metallothionein;(ii) B...
  9. Metallothioneins and polyamines in major depression and suicide
    Adolfo Sequeira; Fiscal Year: 2013
    ..We will determine the effects of cortisol and antidepressants on polyamine, and metallothionein gene and protein expression...
  10. REGULATION OF CELLULAR ZINC HOMEOSTASIS
    David J Eide; Fiscal Year: 2013
    ..by several mechanisms including the control of zinc uptake, efflux, vesicular storage, and binding by metallothionein. In this proposal, another important facet of zinc homeostasis is considered, i.e...
  11. Urinary cadmium and risk of breast cancer
    Jaymie R Meliker; Fiscal Year: 2013
    ..and possible connections to the carcinogenic process, considering interplay between cadmium, zinc, and metallothionein. A third aim will investigate DNA methylation profiles and their association with breast cancer and cadmium, ..
  12. Alcohol Induced Chronic Pancreatitis
    Craig D Logsdon; Fiscal Year: 2013
    ..Aim #3. Determine the role of metallothionein (MT) in the development of CP in alcohol treated mice expressing mutant trypsin...
  13. Molecular Genetics of Cadmium Toxicity
    Daniel W Nebert; Fiscal Year: 2010
    ..Environmental Cd is absorbed by the small intestine and lung, is bound to metallothionein and glutathione in hepatocytes, and is deposited in the kidney...
  14. A Clonable High-Density for 3-D Electron Microscopy of Cellular Structures
    Andreas Hoenger; Fiscal Year: 2010
    ..We propose to develop a metallothionein gene as a "clonable tag" that will bind gold to enhance its density in a variety of samples for EM...
  15. Targeted-and Image-Based Adenovirus Cancer Therapeutic Vectors
    J Michael Mathis; Fiscal Year: 2013
    ..of clinically important cell populations Our innovative use of a structural fusion protein incorporating metallothionein into adenovirus pIX gene provides the non-invasive imaging advantages of detecting physical biodistribution ..
  16. NOT STATED
    Marius Brouwer; Fiscal Year: 1992
    ..The presence of three copper-inducible metallothionein genes in the hepatopancreas have also been demonstrated...
  17. METALLOTHIONEIN, MERCURY AND MONOCYTE ACTIVATION
    RUDOLFS ZALUPS; Fiscal Year: 2006
    ....
  18. CADMIUM TOXICOLOGY
    Curtis Klaassen; Fiscal Year: 2000
    ..Over half of the body burden of Cd is concentrated in liver and kidney and bound to metallothionein (MT). MT is a low molecular weight, cysteine-rich metal-binding protein...
  19. Cadmium Cytoskeletal Toxicology in the Lung
    Iih Nan Chou; Fiscal Year: 2005
    ..In intact cells the MT integrity can be modulated by cellular glutathione (GSH) and metallothionein. Because of their vital roles in many cellular processes, the MT structural impairment could translate into ..
  20. BIOLOGICAL FUNCTION OF COPPER-METALLOTHIONEIN
    Marius Brouwer; Fiscal Year: 1991
    The long-term goal of the proposed research is a better understanding of the role of copper metallothionein (CuMT) in copper metabolism and in metalloenzyme activation...
  21. CADMIUM EFFECTS ON GENE EXPRESSION AND EMBRYOGENESIS
    Glen Andrews; Fiscal Year: 1990
    ..established that the cell's first line of defense against heavy metal toxicity is the metal binding protein metallothionein. Cells also react rapidly to toxic levels of metals by induction of the heat shock proteins (ie HSP-70) which ..
  22. METALLOTHIONEIN ACTIVATION--A MODEL OF CARCINOGEN ACTION
    Michael Lieberman; Fiscal Year: 1991
    ..developed an approach which takes advantage of the fact that mouse S49 cell do not normally express either metallothionein I (MT-I) or metallothionein II (MT-II), although the genes are intact...
  23. HYPOXIC STRESS MECHANISMS IN RADIATION AND CHEMOTHERAPY
    Keith Laderoute; Fiscal Year: 2009
    ..and involved in the transcriptional regulation of a growing number of tumor-related proteins including metallothionein, placenta growth factor, zinc transporter-1, glucose-6-phosphate, dehydrogenase, and matrix ..
  24. Estrogen Receptor-beta and Prostate Function
    Shuk Mei Ho; Fiscal Year: 2004
    ..that express only ER-beta, involves altered expression of survivin, TERT, thymidylate synthase (TS) and metallothionein-II (MT-II)...
  25. Intestinal Endogenous Zinc: Role for Metallothionein?
    Nancy Krebs; Fiscal Year: 2003
    ..The proposed pilot studies examine the potential role of metallothionein (MT) in pancreaticobiliary secretions as a modulator of Zn secretion into the human gastrointestinal tract...
  26. TUMOR IMAGING WITH NUCLIDES THAT BIND TO METALLOTHIONEIN
    Kathryn Morton; Fiscal Year: 1993
    ..an alternative approach to nuclear medicine tumor detection by imaging radiometals which binds many protein metallothionein (MT)...
  27. Metallothionein & Cadmium Carcinogenesis in the Prostate
    Shuk Mei Ho; Fiscal Year: 2006
    ..During our last funding period, we have made several major contributions to the field of Cd and metallothionein research: a) We have developed highly sensitive, sequence-specific quantitative RT-PCR protocols for rodent ..
  28. MECHANISMS OF CADMIUM TOXICITY IN LLC-PK1 CELLS
    WALTER PROZIALECK; Fiscal Year: 1991
    ..The first is to determine whether or not Cd2+ that is bound to metallothionein can disrupt junctions between LLC-PK1 cells in the same manner as Cd2+ itself...
  29. ZINC AND THE SYTHESIS OF ZINC BINDING PROTEINS
    Robert Cousins; Fiscal Year: 1999
    ..that cytokine (interleukin 1) and hormonal regulation of zinc metabolism is mediated through changes in metallothionein gene expression...
  30. MULTINUCLEAR NMR INVESTIGATIONS OF METALLOTHIONEIN
    James Otvos; Fiscal Year: 1980
    The interaction of cadmium with metallothionein, a low molecular weight cysteine-rich cadmium binding protein, will be investigated by 113Cd, 13C, and 1H NMR...
  31. METALLOTHIONEIN IN BIOLOGY AND MEDICINE
    Curtis Klaassen; Fiscal Year: 1990
    ..in the United States, Japan, and Europe to discuss their most recent research results on the role of "Metallothionein in Biology and Medicine"...
  32. METALLOTHIONEIN & HUMAN TUMOR RESISTANCE TO CHEMOTHERAPY
    Marc Ernstoff; Fiscal Year: 1992
    ..This proposal will evaluate the influence of metallothionein (MT) on the efficacy of cDDP as a chemotherapeutic agent in the treatment of human cancers...
  33. METALLOTHIONEIN AND REACTIVE OXYGEN AND NITROGEN SPECIES
    Bruce Pitt; Fiscal Year: 2003
    ..NO induced changes in labile zinc via MPSLM and FRET in perfused lung and zinc depletion or targeted disruption of MT on sensitivity of intact mice to hyperoxia will be studied. ..
  34. DOMINANT-NEGATIVE AND GAIN OF FUNCTION P53 MUTATIONS
    Eric Stanbridge; Fiscal Year: 2000
    ..Using both constitutive and inducible (sheep metallothionein or tetracycline-responsive) promoter constructs, we will manipulate the relative levels of mutant:Wt p53 ..
  35. HYPOXIA AND GENE EXPRESSION IN HUMAN TUMORS
    James Raleigh; Fiscal Year: 2003
    ..account for the expression of oxygen regulated proteins (ORPs) such as vascular endothelial growth factor and metallothionein (MT) in human squamous cell carcinomas in clinical studies...
  36. FLOURESCENT TOOLS FOR THE STUDY OF METALLOTHIONEIN
    Christopher Frederickson; Fiscal Year: 2006
    ..DESCRIPTION (provided by applicant): We will deliver to life scientists fluorescent tools for the study of metallothionein and metallothionein- zinc signaling...
  37. NMR STUDIES OF METALLOTHIONEIN STRUCTURE AND REACTIVITY
    James Otvos; Fiscal Year: 1992
    ..reactivity correlations between the multiple polymorphic forms (isoforms) of the metal binding protein, metallothionein (MT)...
  38. REGULATION OF METALLOTHIONEIN GENE EXPRESSION
    Edward Seto; Fiscal Year: 2002
    ..This regulation is achieved by the presence of multiple, distinct metallothionein genes, the predominant metallothionein gene is hMTIIA, which accounts for 50% of all metallothioneins ..
  39. METALLOTHIONEIN AND PROSTATE TUMORIGENESIS
    ASIM ABDEL MAGEED; Fiscal Year: 2000
    ..Current evidence demonstrated the unequivocal relationship between the ubiquitous occurrence of metallothionein (MT) in various types of human primary tumors, such as prostate cancer, breast carcinoma and malignant ..
  40. METABOLIC DETERMINANTS OF SPONTANEOUS MUTAGENESIS
    Toby Rossman; Fiscal Year: 2000
    ..proposal is to evaluate the ability of the antioxidants Vitamin C (ascorbate), Vitamin E (a-tocopherol) and metallothionein (MT) to reduce the frequency and spectrum of spontaneous mutations at the HPRT locus...
  41. CENTER FOR REPRODUCTIVE SCIENCES
    Paul Terranova; Fiscal Year: 2000
    ..The primary investigators and their research interests are: Glen Andrews, structure/regulation of the metallothionein gene and transcriptional control of cytokines/growth factors in preimplantation embryo development; S.K...
  42. GENETICS OF THIONEIN & METAL TOLERANCE IN DROSOPHILA
    GUSTAVO MARONI; Fiscal Year: 1992
    ..is to obtain an integrated understanding of the functions and regulation of the metal-binding protein metallothionein (MT)...
  43. Human Prolactin antagonist as a Chemopreventive Agent
    Wen Chen; Fiscal Year: 2004
    ..Our specific aims are (1) to establish bi-transgenic mice by cross-breeding male mouse metallothionein I promoter (MT) hPRL transgenic mice (MT/hPRL) and MT/G129R transgenic mice with female MMTV/neu transgenic ..
  44. Regulation of Celluar Zinc Distribution
    Wolfgang Maret; Fiscal Year: 2004
    ..The experiments proposed in this application will investigate the roles of metallothionein (MT) and its apoprotein thionein (T) in this process and the role of the MT/T ratio as a regulated zinc donor/..
  45. METALLOTHIONEIN AND ADRIAMYCIN CARDIOTOXICITY
    Y Kang; Fiscal Year: 2006
    Cardiotoxicity due to oxidative stress compromises effective use of Adriamycin in cancer treatment. Metallothionein (MT), a potent antioxidant, protects the heart from Adriamycin-induced toxicity...
  46. Oxidative Stress and Heart Failure by Copper Restriction
    Y James Kang; Fiscal Year: 2010
    ..Therefore, Cu supplementation is not an appropriate approach to rescue diabetic cardiomyopathy. ..
  47. Environmental Hormones: Effects on Thyroid Function
    Curtis Klaassen; Fiscal Year: 2005
    ..abstract_text> ..
  48. METALLOTHIONEIN IN REPRODUCTION AND DEVELOPMENT
    Glen Andrews; Fiscal Year: 2002
    These studies address research objectives of this RFA including: mechanisms of regulation of metallothionein (MT) gene expression, metal induction during development, and organs specificity in transgenic models...
  49. Neurotoxicology of dietary iron/manganese interactions
    Keith Erikson; Fiscal Year: 2005
    ..If successful, these studies will lay the foundation for the development of a pharmacological therapy aimed at the prevention of manganese-accumulation in vulnerable populations. ..
  50. HEPATOBILIARY DISPOSITION IN TOXICOLOGY
    Curtis Klaassen; Fiscal Year: 2003
    ..abstract_text> ..
  51. Environmental Toxicology Using Transgenic Models
    Glen Andrews; Fiscal Year: 2005
    ..g., the metal chelator metallothionein, the metal exporter zinc-transporter-1, and gamma-glutamylcysteine synthetase heavy chain, the rate limiting ..
  52. Molecular Biology of Mammalian Zinc Homeostasis
    Glen Andrews; Fiscal Year: 2006
    ..Andrews is an expert in mammalian embryonic development and zinc deficiency, and in the molecular biology of metallothionein gene regulation. The co-I., Dr...
  53. A mechanism of metal-mediated immunosuppression
    MICHAEL LYNES; Fiscal Year: 2007
    ..b>Metallothionein (MT) is an intriguing example of this group of proteins, and plays several critical roles in cellular ..
  54. MECHANISMS OF CADMIUM TOXICITY IN EPITHELIAL CELLS
    WALTER PROZIALECK; Fiscal Year: 2007
    ..Furthermore, the results of these studies could have more general implications regarding the mechanisms by which toxic substances affect living organisms. ..
  55. Metal Transfer Reactions between Stable Isotopically Doped Zn Proteins
    Andrew Mason; Fiscal Year: 2007
    ..One class of protein that have been implicated in metal homeostasis is metallothionein (MT)...
  56. The link between lipid peroxidation and manganese induced glutathione depletion
    Keith Erikson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  57. Coordinate Regulation of Uptake and Efflux Transporters
    Curtis D Klaassen; Fiscal Year: 2010
    ....
  58. Zinc and Alcohol-Induced Oxidative Liver Injury
    Zhanxiang Zhou; Fiscal Year: 2009
    ..Cellular zinc homeostasis is regulated by metallothionein (MT). The MT-bound zinc can be released under oxidative stress condition...
  59. Zinc inhibition of endotoxemia in alcoholic liver injury
    Zhanxiang Zhou; Fiscal Year: 2010
    ..The results would have high potential leading to a simplistic approach to prevention/treatment of ALD. ..
  60. Metallothionein in Chronic Alcoholic Liver Injury
    Zhanxiang Zhou; Fiscal Year: 2004
    ..These studies will provide fundamental understanding of the role and possible clinical application of MT in cytoprotection against chronic alcohol-induced liver injury, a major clinical health problem without FDA approved therapy. ..