vesicular transport proteins

Summary

Summary: A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.

Top Publications

  1. pmc Membrane fusion: grappling with SNARE and SM proteins
    Thomas C Sudhof
    Department of Cellular and Molecular Physiology, Stanford University, Palo Alto, CA 94304, USA
    Science 323:474-7. 2009
  2. pmc A mutation in VPS35, encoding a subunit of the retromer complex, causes late-onset Parkinson disease
    Alexander Zimprich
    Department of Neurology, Medizinische Universität Wien, Vienna, Austria
    Am J Hum Genet 89:168-75. 2011
  3. ncbi SNAP receptors implicated in vesicle targeting and fusion
    T Sollner
    Rockefeller Research Laboratory, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    Nature 362:318-24. 1993
  4. pmc VPS35 mutations in Parkinson disease
    Carles Vilariño-Güell
    Department of Medical Genetics, University of British Columbia, Vancouver, Canada
    Am J Hum Genet 89:162-7. 2011
  5. pmc Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
    Matthew N J Seaman
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK
    J Cell Biol 165:111-22. 2004
  6. pmc Exorcising the exocyst complex
    Margaret R Heider
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Traffic 13:898-907. 2012
  7. pmc VPS35 haploinsufficiency increases Alzheimer's disease neuropathology
    Lei Wen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Georgia Health Sciences University, Augusta, GA 30912, USA
    J Cell Biol 195:765-79. 2011
  8. pmc Ubiquitin-dependent regulation of COPII coat size and function
    Lingyan Jin
    Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA
    Nature 482:495-500. 2012
  9. pmc A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis
    Agnes L Nishimura
    Human Genome Research Center, Department of Biology, Biosciences Institute, Sao Paulo University, Sao Paulo, Brazil
    Am J Hum Genet 75:822-31. 2004
  10. pmc LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan
    S Banerji
    University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom
    J Cell Biol 144:789-801. 1999

Research Grants

  1. TRANSPORT OF NEUROTRANSMITTERS INTO SYNAPTIC VESICLES
    Robert Edwards; Fiscal Year: 2005
  2. SYNAPTIC INPUTS TO RETINAL GANGLION CELLS
    RICHARD HARRY MASLAND; Fiscal Year: 2010
  3. APP Mediated Mitochondrial Injury in Aging Neurons
    ANAND HINDUPUR; Fiscal Year: 2007
  4. UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACH
    BHANU JENA; Fiscal Year: 2001
  5. EXOCYTOSIS: USING NANOSURGERY AND BIOENGINEERING
    BHANU JENA; Fiscal Year: 2003
  6. UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACH
    BHANU JENA; Fiscal Year: 2004
  7. Susceptibility mechanism in alcoholic pancreatitis
    Herbert Gaisano; Fiscal Year: 2007
  8. STRUCTURE/FUNCTION OF THE SNARE COMPLEX
    Phyllis Hanson; Fiscal Year: 2002
  9. New Perspectives in Transporter Biology-FASEB conference
    Robert Edwards; Fiscal Year: 2003
  10. GENETIC EPIDEMIOLOGY OF CRANIOSYNOSTOSIS
    SIMEON BOYADJIEV BOYD; Fiscal Year: 2004

Detail Information

Publications292 found, 100 shown here

  1. pmc Membrane fusion: grappling with SNARE and SM proteins
    Thomas C Sudhof
    Department of Cellular and Molecular Physiology, Stanford University, Palo Alto, CA 94304, USA
    Science 323:474-7. 2009
    ....
  2. pmc A mutation in VPS35, encoding a subunit of the retromer complex, causes late-onset Parkinson disease
    Alexander Zimprich
    Department of Neurology, Medizinische Universität Wien, Vienna, Austria
    Am J Hum Genet 89:168-75. 2011
    ..VPS35 is a component of the retromer complex and mediates retrograde transport between endosomes and the trans-Golgi network, and it has recently been found to be involved in Alzheimer disease...
  3. ncbi SNAP receptors implicated in vesicle targeting and fusion
    T Sollner
    Rockefeller Research Laboratory, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    Nature 362:318-24. 1993
    ....
  4. pmc VPS35 mutations in Parkinson disease
    Carles Vilariño-Güell
    Department of Medical Genetics, University of British Columbia, Vancouver, Canada
    Am J Hum Genet 89:162-7. 2011
    ..Our study implicates disruption of VPS35 and retromer-mediated trans-membrane protein sorting, rescue, and recycling in the neurodegenerative process leading to PD...
  5. pmc Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
    Matthew N J Seaman
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK
    J Cell Biol 165:111-22. 2004
    ..The data presented here support the hypothesis that retromer performs a selective function in endosome-to-Golgi transport, mediating retrieval of the CI-MPR, but not furin...
  6. pmc Exorcising the exocyst complex
    Margaret R Heider
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Traffic 13:898-907. 2012
    ..In this review, we discuss current knowledge of exocyst architecture, assembly, regulation and its roles in a variety of cellular trafficking pathways...
  7. pmc VPS35 haploinsufficiency increases Alzheimer's disease neuropathology
    Lei Wen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Georgia Health Sciences University, Augusta, GA 30912, USA
    J Cell Biol 195:765-79. 2011
    ..These results demonstrate an essential role for VPS35 in suppression of AD neuropathology and in inhibition of BACE1 activation and Aβ production by promoting BACE1 endosome-to-Golgi retrieval...
  8. pmc Ubiquitin-dependent regulation of COPII coat size and function
    Lingyan Jin
    Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA
    Nature 482:495-500. 2012
    ..As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat...
  9. pmc A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis
    Agnes L Nishimura
    Human Genome Research Center, Department of Biology, Biosciences Institute, Sao Paulo University, Sao Paulo, Brazil
    Am J Hum Genet 75:822-31. 2004
    ..These data suggest that clinically variable MNDs may be caused by a dysfunction in intracellular membrane trafficking...
  10. pmc LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan
    S Banerji
    University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom
    J Cell Biol 144:789-801. 1999
    ..Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves...
  11. ncbi Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover
    Gianni M Di Guglielmo
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Cell Biol 5:410-21. 2003
    ..Thus, segregation of TGF-beta receptors into distinct endocytic compartments regulates Smad activation and receptor turnover...
  12. ncbi EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ....
  13. pmc Atg32 is a mitochondrial protein that confers selectivity during mitophagy
    Tomotake Kanki
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Dev Cell 17:98-109. 2009
    ..Therefore, Atg32 confers selectivity for mitochondrial sequestration as a cargo and is necessary for recruitment of this organelle by the autophagy machinery for mitophagy...
  14. pmc A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast
    M N Seaman
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla, California 92093 0668, USA
    J Cell Biol 142:665-81. 1998
    ..Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval...
  15. pmc The retromer complex - endosomal protein recycling and beyond
    Matthew N J Seaman
    University of Cambridge, Cambridge Institute for Medical Research, UK
    J Cell Sci 125:4693-702. 2012
    ..In this Commentary, I will highlight recent advances in the understanding of retromer-mediated endosomal protein sorting and discuss how retromer contributes to a diverse set of physiological processes...
  16. ncbi Tethering factors as organizers of intracellular vesicular traffic
    I Mei Yu
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Annu Rev Cell Dev Biol 26:137-56. 2010
    ..The combination of these and other approaches has led to exciting progress toward understanding how these essential nanomachines work...
  17. ncbi Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle
    Marcus C S Lee
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
    Cell 122:605-17. 2005
    ..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
  18. ncbi Protein sorting receptors in the early secretory pathway
    Julia Dancourt
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Annu Rev Biochem 79:777-802. 2010
    ..Our current understanding of the molecular mechanisms underlying cargo receptor function are described...
  19. ncbi Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles
    Elizabeth A Miller
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 114:497-509. 2003
    ..Together, our data support a model whereby Sec24p proteins contain multiple independent cargo binding domains that allow for recognition of a diverse set of sorting signals...
  20. pmc Retromer
    Juan S Bonifacino
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, Building 18T Room 101, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 20:427-36. 2008
    ..Recent studies have implicated retromer in a broad range of physiological, developmental and pathological processes, underscoring the critical nature of retrograde transport mediated by this complex...
  21. pmc A class of dynamin-like GTPases involved in the generation of the tubular ER network
    Junjie Hu
    Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 138:549-61. 2009
    ..Since atlastin-1 mutations cause a common form of hereditary spastic paraplegia, we suggest ER-shaping defects as a neuropathogenic mechanism...
  22. pmc The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics
    Michael E Harbour
    Department of Clinical Biochemistry, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Addenbrookes Hospital, Cambridge CB2 0XY, UK
    J Cell Sci 123:3703-17. 2010
    ....
  23. ncbi Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin
    Y Hata
    Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235
    Nature 366:347-51. 1993
    ..Our data suggest that Munc-18 is a previously unidentified essential component of the synaptic vesicle fusion protein complex...
  24. ncbi The retromer complex influences Wnt secretion by recycling wntless from endosomes to the trans-Golgi network
    Tatyana Y Belenkaya
    Division of Developmental Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Dev Cell 14:120-31. 2008
    ..Our findings link Wls and retromer functions in the same conserved Wnt secretion pathway. We propose that retromer influences Wnt secretion by recycling Wntless from endosomes to the trans-Golgi network (TGN)...
  25. pmc Mycobacterium tuberculosis glycosylated phosphatidylinositol causes phagosome maturation arrest
    Rutilio A Fratti
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 100:5437-42. 2003
    ..These findings identify ManLAM as the M. tuberculosis product responsible for the inhibition of phagosomal maturation...
  26. pmc Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest
    R A Fratti
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Cell Biol 154:631-44. 2001
    ..tuberculosis. This study also identifies mycobacterial phosphoinositides as products with specialized toxic properties, interfering with discrete trafficking stages in phagosomal maturation...
  27. ncbi Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis
    H Chen
    Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Nature 394:793-7. 1998
    ..We propose that epsin may participate, together with Eps15, in the molecular rearrangement of the clathrin coats that are required for coated-pit invagination and vesicle fission...
  28. ncbi The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
    Y Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 414:652-6. 2001
    ..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation...
  29. pmc WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function
    Joshua T Piotrowski
    Department of Immunology, College of Medicine, Mayo Clinic, Rochester, Minnesota, USA
    Mol Cell Biol 33:958-73. 2013
    ..Aberrant trafficking of these key T cell proteins may potentially lead to attenuated proliferation and effector function...
  30. pmc Snapin recruits dynein to BDNF-TrkB signaling endosomes for retrograde axonal transport and is essential for dendrite growth of cortical neurons
    Bing Zhou
    Synaptic Functions Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Building 35, Room 2B 215, 35 Convent Drive, Bethesda, MD 20892 3706, USA
    Cell Rep 2:42-51. 2012
    ..Our study indicates that Snapin-dynein coupling is one of the primary mechanisms driving BDNF-TrkB retrograde transport, thus providing mechanistic insights into the regulation of neuronal growth and survival...
  31. pmc Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons
    Qian Cai
    Synaptic Function Section, The Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892 3706, USA
    Neuron 68:73-86. 2010
    ..Altogether, our study highlights new mechanistic insights into how Snapin-DIC coordinates retrograde transport and late endosomal-lysosomal trafficking critical for autophagy-lysosomal function, and thus neuronal homeostasis...
  32. ncbi Endosomal sorting and signalling: an emerging role for sorting nexins
    Peter J Cullen
    Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 9:574-82. 2008
    ..Here we discuss the sorting nexins family of proteins and propose that they have a fundamental role in orchestrating the formation of protein complexes that are involved in endosomal sorting and signalling...
  33. ncbi Wnt signaling establishes anteroposterior neuronal polarity and requires retromer in C. elegans
    Brinda C Prasad
    Molecular Neurobiology Program, Department of Pharmacology, Skirball Institute, NYU School of Medicine, New York, NY 10016, USA
    Development 133:1757-66. 2006
    ..We show that retromer protein VPS-35 is required in Wnt-expressing cells and propose that retromer activity is needed to generate a fully active Wnt signal...
  34. ncbi Retromer controls epithelial cell polarity by trafficking the apical determinant Crumbs
    Shirin Meher Pocha
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Curr Biol 21:1111-7. 2011
    ..Our data reveal a mechanism for Crb trafficking by retromer that is vital for maintaining Crb levels and localization. We also show a novel function for retromer in maintaining epithelial cell polarity...
  35. pmc Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
    Cecilia N Arighi
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 165:123-33. 2004
    ..These observations indicate that retromer prevents the delivery of the CI-MPR to lysosomes, probably by sequestration into endosome-derived tubules from where the receptor returns to the TGN...
  36. ncbi Relationships between EEA1 binding partners and their role in endosome fusion
    I G Mills
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    J Cell Sci 114:1959-65. 2001
    ..These studies reveal a complex network of interactions between the proteins required for endosome fusion...
  37. ncbi Curvature of clathrin-coated pits driven by epsin
    Marijn G J Ford
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 419:361-6. 2002
    ..We propose that this helix is inserted into one leaflet of the lipid bilayer, inducing curvature. On lipid monolayers epsin alone is sufficient to facilitate the formation of clathrin-coated invaginations...
  38. ncbi A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function
    H Horiuchi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1149-59. 1997
    ..This demonstrates that a soluble exchange factor coupled to a Rab effector translocates from cytosol to the membrane, where the complex stabilizes the GTPase in the active state...
  39. pmc Structural basis for cargo regulation of COPII coat assembly
    Scott M Stagg
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 134:474-84. 2008
    ..The COPII coat structure leads us to propose a mechanism by which cargo drives cage assembly and membrane curvature for budding from the ER...
  40. pmc Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes
    Andreas Vonderheit
    Institute of Biochemistry, Swiss Federal Institute of Technology, Zurich, Switzerland
    PLoS Biol 3:e233. 2005
    ..We concluded that Rab7 is recruited to early endosomes, where it forms distinct domains that mediate cargo sorting as well as the formation of late-endosome-targeted transport vesicles...
  41. pmc Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p
    Charles Boyd
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 167:889-901. 2004
    ..Assembly of the exocyst occurs when the first subset of subunits, delivered on vesicles, joins Sec3p and Exo70p on the plasma membrane. Exocyst assembly serves to both target and tether vesicles to sites of exocytosis...
  42. ncbi Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules
    Suprabha Pulipparacharuvil
    Center for Basic Neuroscience, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9111, USA
    J Cell Sci 118:3663-73. 2005
    ..Ubiquitous knockdown of dVps16A was lethal. Together, these findings demonstrate that Drosophila Vps16A is essential for lysosomal trafficking. Furthermore, metazoans have two types of Vps-C complexes with non-redundant functions...
  43. ncbi The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis
    Karolina Peplowska
    University of Osnabruck, Department of Biology, Biochemistry Section, Barbarastrasse 13, 49076 Osnabruck, Germany
    Dev Cell 12:739-50. 2007
    ..Our data suggest that modular assembled tethering complexes define organelle biogenesis in the endocytic pathway...
  44. ncbi SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment
    Colin J Traer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Cell Biol 9:1370-80. 2007
    ..Finally, these data suggest that by associating with molecular motors, SNX-BAR proteins may coordinate sorting with carrier transport between donor and recipient membranes...
  45. ncbi Rab7: role of its protein interaction cascades in endo-lysosomal traffic
    Tuanlao Wang
    Institute for Biomedical Research, Xiamen University, Xiamen, Fujian, China 361005
    Cell Signal 23:516-21. 2011
    ..We summarize here the key protein interaction cascades of Rab7 by focusing on endo-lysosomal trafficking regulated by its interaction with HOPs, RILP, ORP1L, FYCO1 and Mon1/Sand1-CCZ1 complex...
  46. ncbi Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
    R B Sutton
    The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 395:347-53. 1998
    ..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission...
  47. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    ..In conclusion, we have shown that Golgi-resident glycosylation enzymes recycle through the ER and that this novel pathway is the likely explanation for the nocodazole-induced Golgi scattering observed in interphase cells...
  48. pmc Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure
    Janna Merte
    The Solomon H Snyder Department of Neuroscience and Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, MD 21205, USA
    Nat Cell Biol 12:41-6; sup pp 1-8. 2010
    ..Thus, during COPII vesicle formation, Sec24b shows cargo specificity for a core PCP component, Vangl2, of which proper ER-to-Golgi transport is essential for the establishment of PCP, convergent extension and closure of the neural tube...
  49. ncbi SNAREs and traffic
    Wanjin Hong
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Sinapore, Singapore
    Biochim Biophys Acta 1744:493-517. 2005
    ..In the post-genomic era, a fairly complete list of "all" SNAREs in several organisms (including human) can now be made. This review aims to summarize the key properties and the mechanism of action of SNAREs in mammalian cells...
  50. pmc The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15
    A S Sengar
    Programs of Cancer and Blood Research, and Developmental Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    EMBO J 18:1159-71. 1999
    ..The Ese1-Eps15 scaffold therefore links dynamin, epsin and other endocytic pathway components...
  51. ncbi Anthrax toxins cooperatively inhibit endocytic recycling by the Rab11/Sec15 exocyst
    Annabel Guichard
    Section of Cell and Developmental Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0349, USA
    Nature 467:854-8. 2010
    ..This coordinated disruption of the Rab11/Sec15 exocyst by anthrax toxins may contribute to toxin-dependent barrier disruption and vascular dysfunction during B. anthracis infection...
  52. ncbi SNAREs contribute to the specificity of membrane fusion
    S J Scales
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305, USA
    Neuron 26:457-64. 2000
    ..The data suggest that SNARE pairing does underlie vesicle trafficking fidelity, and that specific SNARE interactions with other proteins may facilitate the correct pairing...
  53. pmc The serotonin transporter is an exclusive client of the coat protein complex II (COPII) component SEC24C
    Sonja Sucic
    Institute of Pharmacology, Center of Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria
    J Biol Chem 286:16482-90. 2011
    ..By inference, variations in the Sec24C gene may also affect SERT cell surface levels and thus be linked to mood disorders...
  54. pmc Cargo selection into COPII vesicles is driven by the Sec24p subunit
    Elizabeth Miller
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720 3202, USA
    EMBO J 21:6105-13. 2002
    ..Our data suggest that the principle role of Sec24p is to discriminate cargo molecules for incorporation into COPII vesicles...
  55. ncbi Structure of the Sec13/31 COPII coat cage
    Scott M Stagg
    National Resource for Automated Molecular Microscopy, Departments of Cell, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 439:234-8. 2006
    ..Our data are consistent with a model for COPII coat complex assembly in which Sec23/24 has a non-structural role as a multivalent ligand localizing the self-assembly of Sec13/31 to form a cage lattice driving ER cargo export...
  56. pmc Helical structures of ESCRT-III are disassembled by VPS4
    Suman Lata
    Unit for Virus Host Cell Interaction, UMR 5233 UJF Université Joseph Fourier EMBL European Molecular Biology Laboratory CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France
    Science 321:1354-7. 2008
    ..Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4...
  57. pmc Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products
    M N Seaman
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    J Cell Biol 137:79-92. 1997
    ....
  58. pmc An exocyst complex functions in plant cell growth in Arabidopsis and tobacco
    Michal Hála
    Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
    Plant Cell 20:1330-45. 2008
    ..We conclude that the exocyst functions as a complex in plant cells, where it plays important roles in morphogenesis...
  59. ncbi Compartmental specificity of cellular membrane fusion encoded in SNARE proteins
    J A McNew
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 407:153-9. 2000
    ..Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis...
  60. pmc Membrane recruitment of the cargo-selective retromer subcomplex is catalysed by the small GTPase Rab7 and inhibited by the Rab-GAP TBC1D5
    Matthew N J Seaman
    University of Cambridge, Cambridge Institute for Medical Research, Department of Clinical Biochemistry, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 0XY, UK
    J Cell Sci 122:2371-82. 2009
    ..We therefore propose that recruitment of the cargo-selective VPS35/29/26 complex is catalysed by Rab7 and inhibited by the Rab-GAP protein, TBC1D5...
  61. pmc Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery
    J A Martina
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 153:1111-20. 2001
    ..These observations suggest that stonin 2 is a novel component of the general endocytic machinery...
  62. pmc Vps-C complexes: gatekeepers of endolysosomal traffic
    Daniel P Nickerson
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Curr Opin Cell Biol 21:543-51. 2009
    ..Accumulating evidence supports the view that Vps-C complexes implement a regulatory logic that governs endomembrane identity and dynamics...
  63. pmc The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p
    Melonnie L M Furgason
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 106:14303-8. 2009
    ..Furthermore, the Tlg2p N-peptide competes with the closed conformation for binding, suggesting a fundamental regulatory mechanism for SM-syntaxin interactions in SNARE assembly and membrane fusion...
  64. ncbi RalA and the exocyst complex influence neuronal polarity through PAR-3 and aPKC
    Giovanna Lalli
    The Wolfson Centre for Age Related Diseases, King s College London, London SE1 1UL, UK
    J Cell Sci 122:1499-506. 2009
    ..Thus, neuronal polarization involves a RalA-regulated association between mediators of vesicle trafficking (exocyst complex) and cell polarity (PAR-3)...
  65. ncbi Recruitment of the endosomal WASH complex is mediated by the extended 'tail' of Fam21 binding to the retromer protein Vps35
    Michael E Harbour
    Cambridge Institute for Medical Research Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 0XY, UK
    Biochem J 442:209-20. 2012
    ..Additionally, overexpression of the Fam21-tail results in cell-spreading defects, implicating the activity of the WASH complex in regulating the mobilization of membrane into the endosome-to-cell surface pathway...
  66. pmc Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1
    Francesca Morgera
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 23:337-46. 2012
    ..Therefore, upon vesicle arrival, Sec6 is proposed to release Sec9 in favor of Sec6-exocyst assembly and to simultaneously recruit Sec1 to sites of secretion for coordinated SNARE complex formation and membrane fusion...
  67. ncbi Vesicle tethering complexes in membrane traffic
    James R C Whyte
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Cell Sci 115:2627-37. 2002
    ..Moreover, the diversity of tethering complexes in the cell suggests that not all tethering events occur through the same mechanisms...
  68. pmc Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions
    Sandra Lopez-Verges
    Institut Cochin, Département Maladies Infectieuses, 27 rue du Faubourg Saint Jacques, F 75014 Paris, France
    Proc Natl Acad Sci U S A 103:14947-52. 2006
    ....
  69. ncbi Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation
    Michael R Lang
    Department of Medicine, Division of Genetic Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Nat Genet 38:1198-203. 2006
    ..As SEC23A lesions cause the cranio-lenticulo-sutural dysplasia syndrome, crusher provides the first vertebrate model system that links the biology of endoplasmic reticulum to Golgi trafficking with a clinically relevant dysmorphology...
  70. ncbi Live imaging of yeast Golgi cisternal maturation
    Kumi Matsuura-Tokita
    Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
    Nature 441:1007-10. 2006
    ....
  71. ncbi A VAMP-binding protein from Aplysia required for neurotransmitter release
    P A Skehel
    Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
    Science 269:1580-3. 1995
    ..Presynaptic injection of antibodies specific for VAP-33 inhibited synaptic transmission, which suggests that VAP-33 is required for the exocytosis of neurotransmitter...
  72. pmc Coordinated lipid transfer between the endoplasmic reticulum and the Golgi complex requires the VAP proteins and is essential for Golgi-mediated transport
    Diego Peretti
    The Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 19:3871-84. 2008
    ..We propose that Nir2, OSBP, and CERT function coordinately at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities...
  73. ncbi Distinct endocytic pathways control the rate and extent of synaptic vesicle protein recycling
    Susan M Voglmaier
    Department of Neurology, Graduate Programs in Cell Biology, Neuroscience and Biomedical Sciences, UCSF School of Medicine, 600 16th Street, San Francisco, California 94143, USA
    Neuron 51:71-84. 2006
    ..After stimulation, however, inhibition of the AP3 pathway prevents the full recovery of VGLUT1 by endocytosis, implicating the AP3 pathway specifically in compensatory endocytosis...
  74. pmc The neuronal sortilin-related receptor SORL1 is genetically associated with Alzheimer disease
    Ekaterina Rogaeva
    Centre for Research in Neurodegenerative Diseases, Department of Medicine, Department, University of Toronto, Toronto, Ontario, Canada
    Nat Genet 39:168-77. 2007
    ..These data suggest that inherited or acquired changes in SORL1 expression or function are mechanistically involved in causing Alzheimer disease...
  75. pmc The amyotrophic lateral sclerosis 8 protein VAPB is cleaved, secreted, and acts as a ligand for Eph receptors
    Hiroshi Tsuda
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 133:963-77. 2008
    ..We propose that VAP MSP domains are secreted and act as diffusible hormones for Eph receptors. This work provides insight into mechanisms that may impact the pathogenesis of ALS...
  76. ncbi ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains
    Alexander A Mironov
    Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Chieti, Italy
    Dev Cell 5:583-94. 2003
    ..Fully protruded saccules then move centripetally, evolving into one of two types of carriers (with distinct kinetic and structural features). These findings provide an alternative framework for analysis of ER-to-Golgi traffic...
  77. ncbi TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking
    E Diaz
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307, USA
    Cell 93:433-43. 1998
    ..These data suggest that TIP47 binds MPR cytoplasmic domains and facilitates their collection into transport vesicles destined for the Golgi...
  78. pmc Identification of two evolutionarily conserved genes regulating processing of engulfed apoptotic cells
    Jason M Kinchen
    Center for Cell Clearance, University of Virginia, Charlottesville, Virginia 22908, USA
    Nature 464:778-82. 2010
    ..Taken together, these data identify SAND-1 (Mon1) and CCZ-1 (Ccz1) as critical and evolutionarily conserved components regulating the processing of ingested apoptotic cell corpses...
  79. ncbi ER stress and unfolded protein response in amyotrophic lateral sclerosis
    Kohsuke Kanekura
    Department of Cell Biology and Neuroscience, School of Medicine, Keio University, Tokyo, 160 8582, Japan
    Mol Neurobiol 39:81-9. 2009
    ..In this review, we summarize recent advances in research on the ALS pathomechanism especially addressing the putative involvement of ER stress and UPR dysfunction...
  80. pmc The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network
    Thomas Wassmer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, UK
    Dev Cell 17:110-22. 2009
    ..These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting...
  81. ncbi Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner
    Giuseppa Pennetta
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Neuron 35:291-306. 2002
    ..We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding...
  82. pmc Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic
    Rachael L Plemel
    Department of Biochemistry, University of Washington, Seattle, WA 98195 3750, USA
    Mol Biol Cell 22:1353-63. 2011
    ..Biochemical and genetic experiments demonstrate the importance of these regions, revealing the Vps11 CTD as a key integrator of Vps-C complex assembly, Rab signaling, and endosomal and lysosomal traffic...
  83. pmc Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex
    Olivier Roumanie
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 170:583-94. 2005
    ..Moreover, our analysis of the Sec3 subunit of the exocyst suggests that polarization of the exocyst may be a consequence rather than a cause of polarized exocytosis...
  84. ncbi A loss-of-function screen reveals SNX5 and SNX6 as potential components of the mammalian retromer
    Thomas Wassmer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
    J Cell Sci 120:45-54. 2007
    ..SNX5 and SNX6 may therefore constitute functional equivalents of Vps17p in mammals...
  85. ncbi New insights into the structural mechanisms of the COPII coat
    Christopher Russell
    Institute for Molecular Biophysics, Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA
    Traffic 11:303-10. 2010
    ..In this review, we will summarize the latest developments in COPII structure and discuss how these structures shed light on the functional mechanisms of the COPII coat...
  86. ncbi Mycobacterium tuberculosis virulence is mediated by PtpA dephosphorylation of human vacuolar protein sorting 33B
    Horacio Bach
    Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, British Columbia V5Z 3J5, Canada
    Cell Host Microbe 3:316-22. 2008
    ..These results demonstrate that PtpA is essential for Mtb intracellular persistence and identify a key host regulatory pathway that is inactivated by Mtb...
  87. ncbi The Mon1-Ccz1 complex is the GEF of the late endosomal Rab7 homolog Ypt7
    Mirjana Nordmann
    Department of Biology Chemistry, University of Osnabruck, Germany
    Curr Biol 20:1654-9. 2010
    ..Our data indicate that the Mon1-Ccz1 complex triggers endosomal maturation by activating Ypt7 on late endosomes...
  88. ncbi Role of Erv29p in collecting soluble secretory proteins into ER-derived transport vesicles
    W J Belden
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Science 294:1528-31. 2001
    ..In vivo, export of gpalphaf from the ER was saturable and depended on the expression level of Erv29p. These results indicate that membrane receptors can link soluble cargo proteins to the COPII coat...
  89. ncbi Vesicle trafficking: pleasure and pain from SM genes
    Ruud F G Toonen
    Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam VUA, De Boelelaan 1087, The Netherlands
    Trends Cell Biol 13:177-86. 2003
    ..This review examines whether a universal principle for the molecular function of SM genes exists and whether the divergence in SM gene function can be related to the unique characteristics of different transport routes...
  90. ncbi Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking
    M Babst
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0668, USA
    Traffic 1:248-58. 2000
    ..This may contribute to the tumorigenic phenotype exhibited by the tsg101 mutant fibroblasts...
  91. pmc Diabetes-associated SorCS1 regulates Alzheimer's amyloid-beta metabolism: evidence for involvement of SorL1 and the retromer complex
    Rachel F Lane
    Department of Neurology and Alzheimer s Disease Research Center, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Neurosci 30:13110-5. 2010
    ..003) in the brains of female Sorcs1 hypomorphic mice. From these data, we propose that dysfunction of SorCS1 may contribute to both the APP/Aβ disturbance underlying AD and the insulin/glucose disturbance underlying DM...
  92. ncbi COPII-cargo interactions direct protein sorting into ER-derived transport vesicles
    M J Kuehn
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA
    Nature 391:187-90. 1998
    ..Our results indicate that cargo packaging signals and soluble cargo adaptors are recognized by a recruitment complex comprising Sar1-GTP and Sec23/24...
  93. ncbi VAPB interacts with and modulates the activity of ATF6
    Christos Gkogkas
    The Centre for Neuroscience Research, The University of Edinburgh, The Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK
    Hum Mol Genet 17:1517-26. 2008
    ..The mis-function of such regulatory systems may contribute to the pathological mechanisms of degenerative motor neuron disease...
  94. pmc Structural basis of cargo membrane protein discrimination by the human COPII coat machinery
    Joseph D Mancias
    Howard Hughes Medical Institute and the Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    EMBO J 27:2918-28. 2008
    ....
  95. ncbi A library of 7TM receptor C-terminal tails. Interactions with the proposed post-endocytic sorting proteins ERM-binding phosphoprotein 50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin 1 (SNX1), and G protein-coupled receptor-associated so
    Arne Heydorn
    Laboratory for Molecular Pharmacology, Department of Pharmacology, Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    J Biol Chem 279:54291-303. 2004
    ..It is concluded that the tail library provides useful information on the general importance of certain adaptor proteins, for example, in this case, ruling out EBP50 as being a broad spectrum-recycling adaptor...
  96. ncbi Controlling the location and activation of Rab GTPases
    Miguel C Seabra
    Cell and Molecular Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, UK
    Curr Opin Cell Biol 16:451-7. 2004
    ..The diversity of cellular processes regulated by Rab family members is made possible, not only by the wide range of effectors they recruit, but also by the different mechanisms regulating their own targeting and activation...
  97. pmc Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p
    X Pan
    Department of Biology, University of Rochester, Rochester NY, 14627, USA
    Mol Biol Cell 11:2445-57. 2000
    ..Overexpression of Nvj1p caused the profound proliferation of NV junctions. We conclude that Vac8p and Nvj1p are necessary components of a novel interorganelle junction apparatus...
  98. pmc Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast
    Felipe O Bendezú
    Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH 1015 Lausanne, Switzerland
    Mol Biol Cell 22:44-53. 2011
    ..Thus in fission yeast long-range cytoskeletal transport and PIP₂-dependent exocyst represent parallel morphogenetic modules downstream of Cdc42, raising the possibility of similar mechanisms in other cell types...
  99. ncbi Stonin 2 is an AP-2-dependent endocytic sorting adaptor for synaptotagmin internalization and recycling
    M Kasim Diril
    Freie Universitat Berlin, Institute of Chemistry and Biochemistry, Department of Membrane Biochemistry, Takustrasse 6, D 14195 Berlin, Germany
    Dev Cell 10:233-44. 2006
    ..Our data identify stonin 2 as an AP-2-dependent endocytic sorting adaptor for synaptotagmin internalization and recycling...
  100. ncbi Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae
    Greg Odorizzi
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Campus Box 347, Boulder, CO 80309, USA
    J Cell Sci 116:1893-903. 2003
    ..Our data suggest that the function of Bro1 in the MVB pathway takes place on endosomal membranes and occurs in concert with or downstream of the function of the ESCRT-III complex...
  101. pmc Phosphatidylinositol 4,5-bisphosphate mediates the targeting of the exocyst to the plasma membrane for exocytosis in mammalian cells
    Jianglan Liu
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Mol Biol Cell 18:4483-92. 2007
    ..Using the ts045 vesicular stomatitis virus glycoprotein trafficking assay, we demonstrate that Exo70-PI(4,5)P(2) interaction is critical for the docking and fusion of post-Golgi secretory vesicles, but not for their transport to the PM...

Research Grants65

  1. TRANSPORT OF NEUROTRANSMITTERS INTO SYNAPTIC VESICLES
    Robert Edwards; Fiscal Year: 2005
    ..We will now use the identified vesicular transport proteins to pursue several goals. First, we will characterize further the ionic mechanisms of transport...
  2. SYNAPTIC INPUTS TO RETINAL GANGLION CELLS
    RICHARD HARRY MASLAND; Fiscal Year: 2010
    ..released from the same cellular sites or different ones? This will be studied by localizing their vesicular transport proteins. The third question is a more general one...
  3. APP Mediated Mitochondrial Injury in Aging Neurons
    ANAND HINDUPUR; Fiscal Year: 2007
    ..function will be investigated in mouse and neuronal models of AD by assessing the interaction of vesicular transport proteins with cytosolic proteins responsible for cargo transport...
  4. UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACH
    BHANU JENA; Fiscal Year: 2001
    ..The biochemical composition of the "new structure" will be determined by microsequencing electrophoretically resolved proteins immunoisolated from brain tissue, using antibodies to the SNARE proteins. ..
  5. EXOCYTOSIS: USING NANOSURGERY AND BIOENGINEERING
    BHANU JENA; Fiscal Year: 2003
    ..These studies will further our understanding of the exocytotic fusion machinery in pancreatic acinar cells, contributing to our understanding of this vital cellular process. ..
  6. UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACH
    BHANU JENA; Fiscal Year: 2004
    ..The biochemical composition of the "new structure" will be determined by microsequencing electrophoretically resolved proteins immunoisolated from brain tissue, using antibodies to the SNARE proteins. ..
  7. Susceptibility mechanism in alcoholic pancreatitis
    Herbert Gaisano; Fiscal Year: 2007
    ..Significance: These actions of alcohol on pancreatic acinar PM-Munc18c may be the 'sentinel' event that initiates the role of alcohol in pancreatic injury, and which could be therapeutically targeted. ..
  8. STRUCTURE/FUNCTION OF THE SNARE COMPLEX
    Phyllis Hanson; Fiscal Year: 2002
    ....
  9. New Perspectives in Transporter Biology-FASEB conference
    Robert Edwards; Fiscal Year: 2003
    ..Each oral session will be followed by a panel discussion of the topic. To promote the advancement of junior faculty, the most exciting poster submissions will be selected for short oral presentations during the regular oral sessions. ..
  10. GENETIC EPIDEMIOLOGY OF CRANIOSYNOSTOSIS
    SIMEON BOYADJIEV BOYD; Fiscal Year: 2004
    ..abstract_text> ..
  11. Host proteins in Vaccinia viral membrane biogenesis
    Victor Hsu; Fiscal Year: 2004
    ..abstract_text> ..
  12. Aggregation and Clearance of Mutant Huntingtin(RMI)
    JAMES ROTHMAN; Fiscal Year: 2005
    ..We hypothesize that by eliminating the accumulated protein we will bring about recovery of the neurodegenerative process, as shown in several animal models of the disease. ..
  13. Regulation of Neurotransmitter Transporter Recyclin(RMI)
    JAMES ROTHMAN; Fiscal Year: 2005
    ..In addition, identification of such molecules may yield compounds that can be developed into new therapeutic drugs, or provide information about potential therapeutic targets. ..
  14. POSTTRANSLATIONAL PROTEIN TRANSPORT INTO YEAST ER
    Tom Rapoport; Fiscal Year: 2003
    ..It will test the hypothesis that this protein works as a molecular ratchet rather than a machine that "pulls" the protein through the membrane. ..
  15. STRUCTURE/FUNCTION OF GTC, A NOVEL GOLGI PROTEIN COMPLEX
    FREDERICK HUGHSON; Fiscal Year: 2002
    ..These studies will provide the first insights into the role of this novel protein complex in the process of intracellular protein traffic. ..
  16. Single Molecule Studies of SNARE-Induced Vesicle Fusion
    Axel T Brunger; Fiscal Year: 2010
    ..Such a system could serve as an efficient model system for novel drug discovery. ..
  17. SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETION
    Robert Flaumenhaft; Fiscal Year: 2007
    ..abstract_text> ..
  18. NONSYNDROMIC CRANIOSYNOSTOSIS: PHENOTYPE/GENOTYPE STUDY
    SIMEON A BOYADJIEV BOYD; Fiscal Year: 2010
    ..In addition, we will also identify genes associated with sagittal NSC. The resources that we have amassed and the collaborative and integrated approaches that we will utilize put us in a unique position to accomplish these aims. ..
  19. Chemical Genetic Analysis of Platelet Granule Secretion
    Robert Flaumenhaft; Fiscal Year: 2008
    ..Once identified, compounds will be tested in a previously established battery of platelet function tests and evaluated for antithrombotic potential in an in vivo murine model of thrombus formation. [unreadable] [unreadable] [unreadable]..
  20. STRUCTURAL ANALYSIS OF INTRACELLULAR TRAFFICKING PROTEIN
    FREDERICK HUGHSON; Fiscal Year: 2002
    ....
  21. Mechanisms of Endosome-to-Lysosome Trafficking in Brain
    Lian Li; Fiscal Year: 2007
    ..abstract_text> ..
  22. Ubiquitin-mediated proteolysis at synaptic terminals
    Lian Li; Fiscal Year: 2007
    ..These studies should advance our understanding of protein degradation at synaptic terminals, and provide a basis for the development of therapeutic strategies for treating malfunctions of the nervous system. ..
  23. Juvenile trophic factors for the prevention and treatment of hippocampal aging
    Jan Krzysztof Blusztajn; Fiscal Year: 2010
    ..The ultimate goal of our studies is to relate our results to the age-associated changes in memory in humans, and to develop juvenile growth-factor replacement strategies that could benefit people. ..
  24. Endocrine pancreatic cell regeneration from bone marrow
    Mehboob A Hussain; Fiscal Year: 2010
    ..4) To identify the cell type within BM that contributes to the pancreatic endocrine cell population. The long-term goal of these studies is to evaluate transplantation of BM derived cells as a potential treatment for diabetes mellitus. ..
  25. Conference on the Diagnosis of Multiple System Atrophy
    Sid Gilman; Fiscal Year: 2006
    ..This conference will assist with early diagnosis by developing new guidelines for diagnosis using newly available imaging and neuropathological criteria. [unreadable] [unreadable] [unreadable]..
  26. DEVELOPMENT OF INTESTINAL TRANSPORT OF CA++ AND PI
    Fayez Ghishan; Fiscal Year: 2007
    ..For Type specifications, see instructions on page6.) RESEARCH GRANT TABLE OF CONTENTS Pago Numbers Face Page -\ Description, ..
  27. Trafficking and signaling of opioid receptor subtypes and heterodimers
    Jennifer L Whistler; Fiscal Year: 2010
    ..Thus targeting these subtypes or heterodimers could provide improved therapeutic utility. ..
  28. Raft/Caveolar Mechanisms in Pulmonary Hypertension
    PRAVIN SEHGAL; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  29. A NOVEL TARGETED THERAPEUTIC USING VIRAL CAPSID PROTEIN
    Lali Medina Kauwe; Fiscal Year: 2008
    ..As HER2+ breast cancer does not respond well to conventional [unreadable] therapies, this alternative therapy could provide a significant contribution to breast cancer treatment. [unreadable] [unreadable] [unreadable]..
  30. ORGANIZATION OF CENTRAL SYMPATHETIC PATHWAYS
    PATRICE GUYENET; Fiscal Year: 2008
    ..The research will contribute to the basic physiological knowledge needed to understand how syrr pathetic tone is regulated in health and in diseases such as arrhythmias, hypertension and dysautonomias. ..
  31. RNAi screen to identify host factors involved in Chlamydia pathogenesis
    Isabelle Derre; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  32. Characterization of a neuronal ubiquitination machinery
    Lian Li; Fiscal Year: 2008
    ..abstract_text> ..
  33. Integrative Biology of Tumor Metastasis
    Rakesh Jain; Fiscal Year: 2009
    ..Finally, we have the resources and the clinical collaborators in place to initiate a clinical trial based on our findings, as attested by our ongoing trial using a VEGF-specific antibody. ..
  34. Retrovirus Restriction by Trima5alpha
    Paul Bieniasz; Fiscal Year: 2009
    ....
  35. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007
    ..Capacity for GSH synthesis, availability of the precursor amino acid cysteine, and steady-state GSH levels all contribute to the body's ability to defend itself against oxidative or chemical stressors. ..
  36. Structure and Function in NSS transporters
    Gary Rudnick; Fiscal Year: 2008
    ..Finally, there are experiments to evaluate the possibility that TnaT functions as a homo-oligomer and to gain structural information about the transporter using two-dimensional (2D) crystallography. ..
  37. Cognition and Estrogen in Menopause:A Monkey Model
    Mary Lou Voytko; Fiscal Year: 2006
    ..The findings from these novel experiments in middle-aged monkeys will significantly advance our understanding of the effects of ovarian hormone therapy on cognitive and neurobiological profiles of primates. ..
  38. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  39. The Role of Doa4 in Membrane Protein Trafficking
    Alexander Amerik; Fiscal Year: 2006
    ..3. Proteins necessary for suppression of the doa4 degradation defect by mutations of Vps factors will be identified by mutagenesis, using chemical and genetic approaches. ..
  40. Rab25 in the Pathogenesis of Ichthyosis Vulgaris
    James Goldenring; Fiscal Year: 2003
    ..In addition, it will provide the first investigations of alterations in membrane recycling in skin diseases. ..
  41. Conference on Angiogenesis in Cancer and Other Diseases
    Rakesh Jain; Fiscal Year: 2002
    ..The meeting's synthesis of available information will be particularly thought-provoking for students and post- doctoral fellows. ..
  42. COMPARTMENTAL ORGANIZATION OF THE YEAST GOLGI COMPLEX
    TODD GRAHAM; Fiscal Year: 2002
    ..In addition, loss of specific Golgi enzyme activities lead to several serious human disorders including I- cell disease, pseudo-Hurler polydystrophy, HEMPAS disease and Lowe syndrome. ..
  43. ADENOSINE DERIVATIVES AND SYNAPTIC TRANSMISSION
    EUGENE SILINSKY; Fiscal Year: 2003
    ..Indeed, fast excitatory purinergic transmission with similar properties to the neurons we study occurs in regions of the CNS associated with cholinergic synapses and with Alzheimer's disease. ..
  44. CELLULAR PHYSIOLOGY OF STAT3
    PRAVIN SEHGAL; Fiscal Year: 2002
    ..Establishing the basic subunit structure of the cytosolic 200-400 kDa and 1-2 MDa STAT3-containing complexes in IL-6-free and IL-6-treated cells is a sine qua non for further progress in this field. ..
  45. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..
  46. Ultraview LCI Spinning Disk Confocal Microscope System
    Dennis Brown; Fiscal Year: 2003
    ..Thus, a wide-range of projects and investigators will benefit from this equipment, which will be integrated into a Core facility that has been operational for over 15 years. ..
  47. NOVEL PROTEINS IN NEUROTRANSMITTER RELEASE
    Lian Li; Fiscal Year: 2001
    ....
  48. IMPROVED CELL COUNTING USING THE OPTICAL DISECTOR
    CHRISTOPHER VON BARTHELD; Fiscal Year: 2004
    ....
  49. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    Stephen Goff; Fiscal Year: 2003
    ..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
  50. Anti-Angiogenesis/Drugs in Tumors--Bench /Bedside & Back
    Rakesh Jain; Fiscal Year: 2005
    ..Attendance at this conference will provide an excellent opportunity for young investigators working on cancer to educate and familiarize themselves with the field of angiogenesis and the complexities of the system. ..
  51. Olympus FV 1000 Confocal Microscope for Neurobiology and Cellular Biology Researc
    Francisco Alvarez; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  52. Nicotine induced neuroplasticity in the carotid body
    Estelle Gauda; Fiscal Year: 2004
    ....
  53. Plasma Membrane Trafficking in Polarized Hepatocytes
    Ann Hubbard; Fiscal Year: 2005
    ..rabs 8, 11 and 17 and the t-SNAREs) as well as candidates identified in Aims #1 B and #2. Our overall goal is to understand the molecular basis of PM protein traffic in polarized hepatocytes. ..
  54. Specificity and Structure: The Kex 2 and Furin Proteases
    Robert Fuller; Fiscal Year: 2005
    ..2. To elucidate the biochemistry and physiology of processing of yeast cell wall proteins by Kex2p and GPI-anchored aspartyl proteases. 3. To achieve Kex2p and furin crystallization to advance determination of their structures. ..
  55. Analysis of sperm transfer behavior in C elegans
    Gary Schindelman; Fiscal Year: 2004
    ..These questions will be investigated by means of surgical and genetic methods. ..
  56. NEUROTRANSMITTER FUNCTION IN C. ELEGANS
    Erik Jorgensen; Fiscal Year: 2004
    ..Ultimately, these studies might help explain the molecular behavior of neuroactive drugs and lead to the development of new and more specific pharmaceuticals which act on individual neurotransmitter systems. ..
  57. Structure and Function of the Phox Domain
    TATIANA KUTATELADZE; Fiscal Year: 2004
    ....
  58. STRUCTURE AND FUNCTION OF FYVE DOMAINS
    TATIANA KUTATELADZE; Fiscal Year: 2004
    ..Zinc's structural importance is demonstrated by FYVE domain refolding experiments, and is related to other zinc binding domains such as RING fingers. ..
  59. The Role of Ceramide Kinase in Neurosecretion
    Sandra Bajjalieh; Fiscal Year: 2002
    ..This work will lay the foundation for biochemical and genetic studies of the role of ceramide kianse, its substrate and its product in neurotransmission and how changes in its activity contribute to synaptic plasticity. ..
  60. The Lymphatic System in the Primate corpus Luteum
    Fuhua Xu; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  61. SYNUCLEIN FUNCTIONS: ROLE IN PARKINSON'S DISEASE
    THOMAS SUDHOF; Fiscal Year: 2003
    ..We anticipate that these experiments will establish a molecular understanding of synuclein functions in the brain and provide insight into the role of an alpha synuclein in Parkinson's disease. ..
  62. APP, Alzheimer's Disease, and Synaptic Function
    THOMAS SUDHOF; Fiscal Year: 2008
    ..Together these studies will contribute to our understanding of APP function and Abeta toxicity in AD, and may suggest new avenues for interfering with the pathogenesis of AD. ..
  63. ICRS SYMPOSIUM ON THE CANNABINOIDS
    ALLYN HOWLETT; Fiscal Year: 2002
    ..Appropriate representation of women has been assured in all aspects of planning, organization and implementation of the annual conferences. ..
  64. FATTY ACID BINDING PROTEINS-LIGAND SPECIFICITY
    Friedhelm Schroeder; Fiscal Year: 2010
    ..Differences in this nuclear receptor/fatty acid energy regulation could contribute to the pathogenesis of obesity, insulin resistance, type 2 diabetes mellitus, and hyperlipidemic conditions. ..