myelin basic proteins

Summary

Summary: A group of 7 proteins produced from a single gene by alternate splicing found in central and peripheral nervous system myelin. The major basic protein (MBP) has long been of interest because it is the antigen, that, when injected into an animal, elicits a cellular immune response that produces the CNS autoimmune disease called experimental allergic encephalomyelitis (ENCEPHALOMYELITIS, ALLERGIC). In the peripheral nervous system, myelin basic protein 18.5-kDa is often referred to as the P1 protein. (From Siegel et al., Basic Neurochemistry, 5th ed, p130, 133)

Top Publications

  1. ncbi Myelin basic protein: a multifunctional protein
    J M Boggs
    Department of Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
    Cell Mol Life Sci 63:1945-61. 2006
  2. ncbi Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis
    George Harauz
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, Room 230, Axelrod Building, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Micron 35:503-42. 2004
  3. pmc T helper cell type 1 (Th1), Th2 and Th17 responses to myelin basic protein and disease activity in multiple sclerosis
    Chris J Hedegaard
    Institute for Inflammation Research, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark
    Immunology 125:161-9. 2008
  4. ncbi Protection of p27(Kip1) mRNA by quaking RNA binding proteins promotes oligodendrocyte differentiation
    Daniel Larocque
    Terry Fox Molecular Oncology Group and the Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    Nat Neurosci 8:27-33. 2005
  5. pmc Interaction of the 18.5-kD isoform of myelin basic protein with Ca2+ -calmodulin: effects of deimination assessed by intrinsic Trp fluorescence spectroscopy, dynamic light scattering, and circular dichroism
    David S Libich
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Protein Sci 12:1507-21. 2003
  6. pmc A public T cell clonotype within a heterogeneous autoreactive repertoire is dominant in driving EAE
    Juscilene S Menezes
    Division of Immune Regulation, Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA
    J Clin Invest 117:2176-85. 2007
  7. ncbi A distal upstream enhancer from the myelin basic protein gene regulates expression in myelin-forming schwann cells
    R Forghani
    Laboratory of Developmental Biology, Department of Neurology and Neurosurgery, Molecular Oncology Group H-5, McGill University, Montreal, Quebec, Canada, H3A 1A1
    J Neurosci 21:3780-7. 2001
  8. ncbi Curing CNS autoimmune disease with myelin-reactive Foxp3+ Treg
    Leigh A Stephens
    Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, Edinburgh, UK
    Eur J Immunol 39:1108-17. 2009
  9. pmc Sodium channel expression and localization at demyelinated sites in painful human dental pulp
    Michael A Henry
    Department of Endodontics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    J Pain 10:750-8. 2009
  10. pmc Mice deficient for the type II transmembrane serine protease, TMPRSS1/hepsin, exhibit profound hearing loss
    Michel Guipponi
    Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
    Am J Pathol 171:608-16. 2007

Detail Information

Publications252 found, 100 shown here

  1. ncbi Myelin basic protein: a multifunctional protein
    J M Boggs
    Department of Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
    Cell Mol Life Sci 63:1945-61. 2006
    ..Further study of this very abundant protein will reveal how it is utilized by the oligodendrocyte and myelin for different purposes...
  2. ncbi Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis
    George Harauz
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, Room 230, Axelrod Building, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Micron 35:503-42. 2004
    ..By solving the 3D structure of 18.5 kDa MBP and the effects of PTMs, we will attain a better understanding of myelin architecture, and of the molecular mechanisms that transpire in demyelinating diseases such as multiple sclerosis...
  3. pmc T helper cell type 1 (Th1), Th2 and Th17 responses to myelin basic protein and disease activity in multiple sclerosis
    Chris J Hedegaard
    Institute for Inflammation Research, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark
    Immunology 125:161-9. 2008
    ..04 and P=0.007). These data suggest that autoantigen-driven CD4+ T-cell proliferation and release of IL-17 and IL-5 may be associated with disease activity. Larger studies are needed to confirm this...
  4. ncbi Protection of p27(Kip1) mRNA by quaking RNA binding proteins promotes oligodendrocyte differentiation
    Daniel Larocque
    Terry Fox Molecular Oncology Group and the Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    Nat Neurosci 8:27-33. 2005
    ..Our findings demonstrate that QKI is upstream of p27(Kip1) during oligodendrocyte differentiation...
  5. pmc Interaction of the 18.5-kD isoform of myelin basic protein with Ca2+ -calmodulin: effects of deimination assessed by intrinsic Trp fluorescence spectroscopy, dynamic light scattering, and circular dichroism
    David S Libich
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Protein Sci 12:1507-21. 2003
    ....
  6. pmc A public T cell clonotype within a heterogeneous autoreactive repertoire is dominant in driving EAE
    Juscilene S Menezes
    Division of Immune Regulation, Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA
    J Clin Invest 117:2176-85. 2007
    ..These results are consistent with the hypothesis that the public clonotype BV8S2/BJ2S7 is a driver of disease and necessary for its propagation...
  7. ncbi A distal upstream enhancer from the myelin basic protein gene regulates expression in myelin-forming schwann cells
    R Forghani
    Laboratory of Developmental Biology, Department of Neurology and Neurosurgery, Molecular Oncology Group H-5, McGill University, Montreal, Quebec, Canada, H3A 1A1
    J Neurosci 21:3780-7. 2001
    ....
  8. ncbi Curing CNS autoimmune disease with myelin-reactive Foxp3+ Treg
    Leigh A Stephens
    Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, Edinburgh, UK
    Eur J Immunol 39:1108-17. 2009
    ....
  9. pmc Sodium channel expression and localization at demyelinated sites in painful human dental pulp
    Michael A Henry
    Department of Endodontics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    J Pain 10:750-8. 2009
    ..This remodeling of NaChs included prominent NaCh expression within nerve regions that showed a selective loss of MBP staining in a pattern consistent with a demyelinating process...
  10. pmc Mice deficient for the type II transmembrane serine protease, TMPRSS1/hepsin, exhibit profound hearing loss
    Michel Guipponi
    Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
    Am J Pathol 171:608-16. 2007
    ..Hearing impairment present in Tmprss1-null mice is characterized by a combination of various structural, cellular, and molecular abnormalities that are likely to affect different cochlear processes...
  11. ncbi Migratory activity and functional changes of green fluorescent effector cells before and during experimental autoimmune encephalomyelitis
    A Flügel
    Max Planck Institute of Neurobiology, Department of Neuroimmunology, 82152 Martinsried, 10098, Berlin, Germany
    Immunity 14:547-60. 2001
    ..Within the CNS, the effector cells are reactivated, with upregulated proinflammatory cytokines and downmodulated T cell receptor-associated structures, presumably reflecting autoantigen recognition in situ...
  12. ncbi Transgenic mice that express a myelin basic protein-specific T cell receptor develop spontaneous autoimmunity
    J Goverman
    Division of Biology, California Institute of Technology, Pasadena 91125
    Cell 72:551-60. 1993
    ..This model system affords a unique opportunity to dissect the genetic and environmental variables that may contribute to the development of spontaneous autoimmune disease...
  13. ncbi Progesterone and its metabolites increase myelin basic protein expression in organotypic slice cultures of rat cerebellum
    A M Ghoumari
    INSERM U488, Bicetre, France
    J Neurochem 86:848-59. 2003
    ....
  14. pmc Kif1b is essential for mRNA localization in oligodendrocytes and development of myelinated axons
    David A Lyons
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA, USA
    Nat Genet 41:854-8. 2009
    ..Our data demonstrate previously unknown functions of kif1b in vivo and provide insights into its possible roles in MS...
  15. ncbi Effect of phosphorylation of myelin basic protein by MAPK on its interactions with actin and actin binding to a lipid membrane in vitro
    Joan M Boggs
    Division of Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Biochemistry 45:391-401. 2006
    ..Thus, phosphorylation of MBP, which occurs in response to various extracellular signals in both myelin and oligodendrocytes, attenuates the ability of MBP to polymerize and bundle actin and to bind it to a negatively charged membrane...
  16. ncbi Upregulation of oligodendrocyte progenitor cells associated with restoration of mature oligodendrocytes and myelination in peri-infarct area in the rat brain
    Kortaro Tanaka
    Department of Neurology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Brain Res 989:172-9. 2003
    ..These findings suggest that the upregulation of OPCs may contribute to replenishment of OLGs and resultant remyelination in the peri-infarct area after ischemic insult...
  17. ncbi Lysolecithin induces demyelination in vitro in a cerebellar slice culture system
    Eric Birgbauer
    Department of Neurobiology, Merck Research Laboratories, San Diego, California, USA
    J Neurosci Res 78:157-66. 2004
    ..Lysolecithin thus induces demyelination in an in vitro organotypic cerebellar slice culture system, providing a model system for studying myelination, demyelination, and remyelination in vitro...
  18. pmc Regulatory CD8+ T cells fine-tune the myelin basic protein-reactive T cell receptor V beta repertoire during experimental autoimmune encephalomyelitis
    H Jiang
    College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 100:8378-83. 2003
    ..Importantly, the T cell clones that expand in the absence of CD8+ T cell control are enriched in potentially pathogenic self-reactive T cell clones capable of inducing EAE in vivo...
  19. ncbi Steroid protection in the experimental autoimmune encephalomyelitis model of multiple sclerosis
    Laura Garay
    Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina Experimental CONICET, Buenos Aires, Argentina
    Neuroimmunomodulation 15:76-83. 2008
    ..As both estradiol (E(2)) and progesterone exert neuroprotective and myelinating effects on the nervous system, the effects of sex steroids were studied in the experimental autoimmune encephalomyelitis (EAE) model of MS...
  20. ncbi Expression of ABCA2 protein in human vestibular schwannoma and peripheral nerve
    Yan Wang
    Department of Physiology, Akita University School of Medicine, 1 1 1 Hondo, Akita 010 8543, Japan
    J Neurol Sci 232:59-63. 2005
    ..These data suggest that ABCA2 may be useful as a marker for cellular characterization of schwannomas...
  21. pmc Reactivity to myelin antigens in multiple sclerosis. Peripheral blood lymphocytes respond predominantly to myelin oligodendrocyte glycoprotein
    N Kerlero de Rosbo
    Neuroimmunology Laboratory, La Trobe University, Bundoora, Australia
    J Clin Invest 92:2602-8. 1993
    ..A predominant T cell reactivity to MOG in MS suggests an important role for cell-mediated immune response to this antigen in the pathogenesis of MS...
  22. pmc Autoantibodies to myelin basic protein (MBP) in healthy individuals and in patients with multiple sclerosis: a role in regulating cytokine responses to MBP
    Chris J Hedegaard
    Institute for Inflammation Research, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
    Immunology 128:e451-61. 2009
    ..However, the latter seem to be more efficient in facilitating the production of IFN-gamma and IL-5...
  23. ncbi Solution NMR and CD spectroscopy of an intrinsically disordered, peripheral membrane protein: evaluation of aqueous and membrane-mimetic solvent conditions for studying the conformational adaptability of the 18.5 kDa isoform of myelin basic protein (MBP)
    David S Libich
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, N1G 2W1, Guelph, ON, Canada
    Eur Biophys J 37:1015-29. 2008
    ..5 at 300 K, and 100 mM KCl, pH 6.5 at 277 K were the best conditions to use for future solution NMR studies of MBP. Micelles of DPC were found to be inappropriate for backbone resonance assignments of rmMBP in this instance...
  24. ncbi FTY720, sphingosine 1-phosphate receptor modulator, ameliorates experimental autoimmune encephalomyelitis by inhibition of T cell infiltration
    Hirotoshi Kataoka
    Research Laboratory III Immunology, Pharmaceutical Research Unit, Research and Development Division, Mitsubishi Pharma Corporation, Yokohama, Japan
    Cell Mol Immunol 2:439-48. 2005
    ....
  25. ncbi Proliferation and death of oligodendrocytes and myelin proteins are differentially regulated in male and female rodents
    Mirela Cerghet
    Department of Neurology, Henry Ford Hospital, Detroit, Michigan 48202, USA
    J Neurosci 26:1439-47. 2006
    ..We discuss the potential significance of these differences to multiple sclerosis, a sexually dimorphic disease, whose progression is altered by exogenous hormones...
  26. pmc The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors
    M C Marty
    Centre National de la Recherche Scientifique, 15 rue de l Ecole de Médecine 75270 Paris CEDEX 06 France
    Proc Natl Acad Sci U S A 99:8856-61. 2002
    b>Myelin basic proteins (MBP) are major constituents of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively...
  27. pmc Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits
    Rosanna Pescini Gobert
    Merck Serono SA, 9, Chemin des Mines, 1211 Geneva, Switzerland
    Mol Cell Biol 29:1538-53. 2009
    ..Oligodendrocytic differentiation feedback loops may therefore yield pharmacological targets to treat disease related to dysfunctional myelin deposition...
  28. ncbi T-cell reactivity to multiple myelin antigens in multiple sclerosis patients and healthy controls
    N Hellings
    Biomedisch Onderzoeksintituut DWI, Limburgs Universitair Centrum, Diepenbeek, Belgium
    J Neurosci Res 63:290-302. 2001
    ..Our data further indicate that precursor frequency analysis of myelin reactive T-cells by proliferation-based assays may underestimate the true frequency of myelin specific T-cells significantly...
  29. ncbi Increased CD8+ cytotoxic T cell responses to myelin basic protein in multiple sclerosis
    Ying C Q Zang
    Multiple Sclerosis Research Unit, Department of Neurology and Baylor Multiple Sclerosis Center, Baylor College of Medicine, Houston, TX 77030, USA
    J Immunol 172:5120-7. 2004
    ..These findings support the potential role of CD8(+) CTLs recognizing MBP in the injury of oligodendrocytes expressing both MHC class I molecules and MBP...
  30. ncbi Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro
    Lopamudra Homchaudhuri
    Department of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8
    Biochemistry 48:2385-93. 2009
    ....
  31. ncbi Contribution of changes in ubiquitin and myelin basic protein to age-related cognitive decline
    Deng Shun Wang
    Department of Neuroscience and Pathology Neuropathology, Birdsall 317, Mayo Clinic Jacksonville, 4500 Sam Pablo Road, Jacksonville, FL 32224, USA
    Neurosci Res 48:93-100. 2004
    ..An age-related decrease in MBP immunoreactivity was detected in NCI cases (r=0.71). These results support the hypothesis that white matter pathology may contribute to age-associated decline in cognition...
  32. ncbi Nitric oxide is a potential down-regulating molecule in autoimmune disease: inhibition of nitric oxide production renders PVG rats highly susceptible to EAE
    W B Cowden
    Division of Immunology and Cell Biology, The John Curtin School of Medical Research, The Australian National University, Canberra ACT
    J Neuroimmunol 88:1-8. 1998
    ..untreated rats could account for their increased susceptibility to developing clinical EAE. The findings in this study suggest that NO plays a role in protecting PVG rats against developing EAE...
  33. ncbi Structural analysis of the complex between calmodulin and full-length myelin basic protein, an intrinsically disordered molecule
    Viivi Majava
    Department of Biochemistry, University of Oulu, PO Box 3000, 90014, Oulu, Finland
    Amino Acids 39:59-71. 2010
    ..Our results provide a detailed picture of the MBP-CaM interaction, including a 3D model of the complex between full-length proteins...
  34. ncbi Charge effects modulate actin assembly by classic myelin basic protein isoforms
    Christopher M D Hill
    Department of Molecular and Cellular Biology, Biophysics Interdepartmental Group, University of Guelph, Guelph, Ont, Canada N1G 2W1
    Biochem Biophys Res Commun 329:362-9. 2005
    ..The presence of at least one region derived from exon II or VI of the "classic" MBP gene was required for effective bundling as assessed by light scattering and transmission electron microscopy...
  35. ncbi IL-10-secreting regulatory T cells do not express Foxp3 but have comparable regulatory function to naturally occurring CD4+CD25+ regulatory T cells
    Pedro L Vieira
    Division of Immunoregulation, The National Institute for Medical Research, London, United Kingdom
    J Immunol 172:5986-93. 2004
    ..These data show that Foxp3 expression is not a prerequisite for IL-10-T(Reg) activity in vitro or in vivo, and suggest that IL-10-T(Reg) and naturally occurring CD4(+)CD25(+) T(Reg) may have distinct origins...
  36. ncbi Remyelination is extensive in a subset of multiple sclerosis patients
    Peter Patrikios
    Center for Brain Research, Medical University of Vienna, Vienna, Austria
    Brain 129:3165-72. 2006
    ..These results suggest that the variable and patient-dependent extent of remyelination must be considered in the design of future clinical trials aimed at promoting CNS repair...
  37. ncbi A functional and structural basis for TCR cross-reactivity in multiple sclerosis
    Heather L E Lang
    Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Nat Immunol 3:940-3. 2002
    ..This provides structural evidence for molecular mimicry involving HLA molecules. The structural details suggest an explanation for the preponderance of MHC class II associations in HLA-associated diseases...
  38. ncbi Acute rolipram/thalidomide treatment improves tissue sparing and locomotion after experimental spinal cord injury
    Guido C Koopmans
    Department of Anesthesiology, Academic Hospital Maastricht, Maastricht, 6200 AZ, The Netherlands
    Exp Neurol 216:490-8. 2009
    ..The present data suggest that clinical trials for the acute combined application of rolipram and thalidomide may be warranted. The use of such "established drugs" could facilitate the early initiation of trials...
  39. pmc Serum levels of anti-myelin antibodies in relapsing-remitting multiple sclerosis patients during different phases of disease activity and immunomodulatory therapy
    Francesco Angelucci
    Institute of Neurology, Department of Neuroscience, Catholic University, Largo Gemelli 8, 00168, Rome, Italy
    Dis Markers 21:49-55. 2005
    ..In conclusion, these data indicate that the use of peripheral levels of autoantibodies against MOG and MBP as marker of multiple sclerosis might be complicated by the phase of disease activity and by the epitope of the MOG protein used...
  40. pmc Crosspresentation by nonhematopoietic and direct presentation by hematopoietic cells induce central tolerance to myelin basic protein
    Antoine Perchellet
    Department of Immunology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 105:14040-5. 2008
    ....
  41. pmc Thyroid hormone activates oligodendrocyte precursors and increases a myelin-forming protein and NGF content in the spinal cord during experimental allergic encephalomyelitis
    Laura Calza
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy
    Proc Natl Acad Sci U S A 99:3258-63. 2002
    ....
  42. ncbi Functional organization of a Schwann cell enhancer
    Eric Denarier
    Laboratory of Developmental Biology, Molecular Oncology Group H 5, McGill University Health Centre, Montreal, Quebec, H3A 1A1, Canada
    J Neurosci 25:11210-7. 2005
    ..Finally, this investigation demonstrates that in vivo functional analysis, supported by controlled transgenesis, can be a robust complement to molecular and bioinformatics approaches to regulatory mechanisms...
  43. ncbi Interaction of lipid-bound myelin basic protein with actin filaments and calmodulin
    J M Boggs
    Division of Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Biochemistry 39:7799-806. 2000
    ..This change in interaction of MBP with the bilayer could then create a cytosol to membrane signal caused by changes in interaction of the cytoskeleton with the membrane...
  44. ncbi Assembly of tubulin by classic myelin basic protein isoforms and regulation by post-translational modification
    Christopher M D Hill
    Department of Molecular and Cellular Biology and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1
    Biochemistry 44:16672-83. 2005
    ..Finally, bC1 was shown to disrupt and aggregate planar sheets of crystalline tubulin stabilized by paclitaxel, establishing that these structures are not suitable substrates for the formation of MBP cocrystals...
  45. ncbi Mutations in the rat myelin basic protein gene are associated with specific alterations in other myelin gene expression
    Jean Luc Carre
    Service de Biochimie et Biologie Moléculaire, Faculte de Medecine, 22 avenue C Desmoulins, 29200 Brest, France
    Neurosci Lett 330:17-20. 2002
    ..Quantitation of mRNA levels indicated that decreases in PLP and DM20 were a result of changes in mRNA levels but detected no change in other myelin gene transcripts...
  46. ncbi Nogo-A expression in the human hippocampus in normal aging and in Alzheimer disease
    Vanessa Gil
    Development and Regeneration of the CNS, IRB PCB, Barcelona Science Park, University of Barcelona, Spain
    J Neuropathol Exp Neurol 65:433-44. 2006
    ..These findings also indicate that Reticulon proteins could be considered as new putative drug targets in therapies of neurodegenerative disorders...
  47. pmc Structured functional domains of myelin basic protein: cross talk between actin polymerization and Ca(2+)-dependent calmodulin interaction
    Vladimir V Bamm
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    Biophys J 101:1248-56. 2011
    ..These three domains of MBP represent molecular recognition fragments with multiple roles in both membrane- and protein-association...
  48. ncbi Purification of a new clathrin assembly protein from bovine brain coated vesicles and its identification as myelin basic protein
    K Prasad
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 0301, USA
    J Biol Chem 270:30551-6. 1995
    ..It is not clear if it acts as an assembly protein in vivo, but because it is well characterized and easily available, MBP will be a useful protein to investigate the mechanism of clathrin assembly and disassembly in vitro...
  49. pmc Degradation of amyloid beta protein by purified myelin basic protein
    Mei Chen Liao
    Department of Neurosurgery, Stony Brook University, Stony Brook, New York 11794 8122, USA
    J Biol Chem 284:28917-25. 2009
    ..Together, these findings indicate that purified MBP possesses Abeta degrading activity in vitro...
  50. ncbi CD28 costimulation is crucial for the development of spontaneous autoimmune encephalomyelitis
    A J Oliveira-dos-Santos
    Amgen Institute, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Ontario, Canada
    J Immunol 162:4490-5. 1999
    ..These results provide the first genetic evidence that CD28 costimulation is crucial for MBP-specific T cell activation in vivo and the initiation of spontaneous EAE...
  51. ncbi Myelin basic protein binds to and inhibits the fibrillar assembly of Abeta42 in vitro
    Michael D Hoos
    Department of Medicine, Stony Brook University, Stony Brook, New York 11794 8153, USA
    Biochemistry 48:4720-7. 2009
    ..These findings suggest that MBP may play a role in regulating the deposition of Abeta42 and thereby also may regulate the early formation of amyloid plaques in Alzheimer's disease...
  52. ncbi Binding of the proline-rich segment of myelin basic protein to SH3 domains: spectroscopic, microarray, and modeling studies of ligand conformation and effects of posttranslational modifications
    Eugenia Polverini
    Dipartimento di Fisica and CNISM, Universita di Parma, V le Usberti, 7 A, 43100 Parma, Italy
    Biochemistry 47:267-82. 2008
    ....
  53. pmc P(0) glycoprotein overexpression causes congenital hypomyelination of peripheral nerves
    L Wrabetz
    Department of Neurology and Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Cell Biol 148:1021-34. 2000
    ..These data reveal new points in nerve development at which Schwann cells are susceptible to increased gene dosage, and suggest a novel basis for hereditary neuropathy...
  54. ncbi Immunochemical studies of myelin basic protein in shiverer mouse devoid of major dense line of myelin
    P Dupouey
    Neurosci Lett 12:113-8. 1979
    ..Indirect immunofluorescence is negative when using specific anti-MBP serum. The importance of Shi/Shi (as compared to other hypomelinating mutants) stems from the specificity of this genetic lesion, i.e. the lack of basic protein...
  55. ncbi Amelioration of relapsing experimental autoimmune encephalomyelitis with altered myelin basic protein peptides involves different cellular mechanisms
    A Gaur
    Neurocrine Biosciences, San Diego, CA 92121, USA
    J Neuroimmunol 74:149-58. 1997
    ..Thus, single amino acid changes at different positions in the same peptide epitope can lead to APL capable of controlling auto-immune disease by different mechanisms...
  56. ncbi Thyroid hormone affects Schwann cell and oligodendrocyte gene expression at the glial transition zone of the VIIIth nerve prior to cochlea function
    M Knipper
    University of Tubingen, Department of Oto Rhino Laryngology, Tübingen Centre for Hearing Research, Röntgenweg 11, D 72076 Tubingen, Germany
    Development 125:3709-18. 1998
    ..The thyroid hormone-dependent commencement of nerve conduction is discussed in connection with the patterning refinement of central auditory pathways and the acquisition of deafness...
  57. ncbi Cutting edge: myelin basic protein-specific cytotoxic T cell tolerance is maintained in vivo by a single dominant epitope in H-2k mice
    E S Huseby
    Department of Immunology, University of Washington, Seattle 98195, USA
    J Immunol 163:1115-8. 1999
    ..This study is the first to show that the pool of self-reactive lymphocytes specific for MBP contain MHC class I-restricted T cells whose response is regulated in vivo by the induction of tolerance...
  58. pmc Tyrosine phosphorylation of QKI mediates developmental signals to regulate mRNA metabolism
    Youyi Zhang
    Department of Pharmacology, Emory University School of Medicine, Atlanta, GA 30322, USA
    EMBO J 22:1801-10. 2003
    ..Therefore, developmental regulation of Src-PTK-dependent tyrosine phosphorylation of QKI suggests a novel mechanism for accelerating CNS myelinogenesis via regulating mRNA metabolism...
  59. ncbi Macrophages and nitric oxide as the possible cellular and molecular basis for strain and gender differences in susceptibility to autoimmune central nervous system inflammation
    Maria A Staykova
    The Neurosciences Research Unit, The Canberra Hospital, Australia
    Immunol Cell Biol 80:188-97. 2002
    ....
  60. pmc Crystal structure of HLA-DR2 (DRA*0101, DRB1*1501) complexed with a peptide from human myelin basic protein
    K J Smith
    Department of Molecular Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
    J Exp Med 188:1511-20. 1998
    ..The necessary space for this aromatic side chain is created by an alanine at the polymorphic DRbeta 71 position. These features make the P4 pocket of HLA-DR2 distinct from DR molecules associated with other autoimmune diseases...
  61. pmc Glatiramer acetate (Copaxone) induces degenerate, Th2-polarized immune responses in patients with multiple sclerosis
    P W Duda
    Laboratory of Molecular Immunology, Center for Neurologic Diseases, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 105:967-76. 2000
    ..This compound may be useful in a variety of autoimmune disorders in which immune deviation to a Th2 type of response is desirable...
  62. ncbi TNF accelerates the onset but does not alter the incidence and severity of myelin basic protein-induced experimental autoimmune encephalomyelitis
    G Kassiotis
    Laboratory of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece
    Eur J Immunol 29:774-80. 1999
    ..Taken together, these results indicate that although TNF is not required for the progression of MBP-induced EAE, it contributes positively by advancing the onset of disease...
  63. ncbi Conformation of bovine myelin basic protein purified with bound lipids
    E Polverini
    Istituto Nazionale per la Fisica della Materia, Universita di Parma, Italy
    Eur Biophys J 28:351-5. 1999
    ..Theoretical predictions, made using different computational methods, substantially confirm the tendency of the protein to assume an ordered secondary structure in accordance with our CD results...
  64. ncbi Activated cyclic AMP-response element binding protein (CREB) is expressed in a myelin-associated protein in chick
    Dong Woon Kim
    Department of Anatomy, Seoul National University College of Medicine, Seoul, South Korea
    Neurochem Res 30:1133-7. 2005
    ..Immunoblot analyses showed that phospho-CREB recognized a protein with molecular weights of approximately 70 kDa. Our data suggest that the antigen recognized by phospho-CREB is a myelin-associated protein in the chick CNS...
  65. ncbi Effect of posttranslational modifications to myelin basic protein on its ability to aggregate acidic lipid vesicles
    J M Boggs
    Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
    Biochemistry 36:5065-71. 1997
    ..However, charge modifications might affect adhesion in cytoplasm-containing regions of myelin such as the paranodal loops, where MBP concentration is low and where K+ concentration may vary in the range of 60-75 mM...
  66. ncbi Interaction of myelin basic protein with actin in the presence of dodecylphosphocholine micelles
    Vladimir V Bamm
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Biochemistry 49:6903-15. 2010
    ..Thus, whereas the N-terminal domain of MBP can be more important for the MBP's actin polymerization activity and membrane-association, the C-terminal domain can regulate its interaction with actin...
  67. ncbi Secondary structure and solvent accessibility of a calmodulin-binding C-terminal segment of membrane-associated myelin basic protein
    Lopamudra Homchaudhuri
    Department of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
    Biochemistry 49:8955-66. 2010
    ..The deiminated form would be more susceptible to regulation of its membrane binding functions by Ca(2+)-CaM than the unmodified form...
  68. pmc Characterization of mouse myelin basic protein messenger RNAs with a myelin basic protein cDNA clone
    N K Zeller
    Proc Natl Acad Sci U S A 81:18-22. 1984
    ..In addition to these, there are also minor bands that hybridize with pNZ111, including a band of 4,100 bases and smaller ones of 1,900, 1,500, and 1,200 bases...
  69. ncbi The microtubule-associated protein tumor overexpressed gene/cytoskeleton-associated protein 5 is necessary for myelin basic protein expression in oligodendrocytes
    Victor P Francone
    Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Neurosci 27:7654-62. 2007
    ..These data indicate that TOG is necessary for efficient translation of MBP mRNA and suggest that this role is mediated by its interaction with hnRNP A2...
  70. ncbi An Arg/Lys-->Gln mutant of recombinant murine myelin basic protein as a mimic of the deiminated form implicated in multiple sclerosis
    Ian R Bates
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Protein Expr Purif 25:330-41. 2002
    ..Overall, the mutant protein's properties were consistent with the effects of deimination and support its use as a model for evaluating the effects of this modification...
  71. pmc CD4(+) T cells prevent spontaneous experimental autoimmune encephalomyelitis in anti-myelin basic protein T cell receptor transgenic mice
    F Van de Keere
    Howard Hughes Medical Institute, The Center for Cancer Research, and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Exp Med 188:1875-82. 1998
    ..Transfer of CD4(+) T cells after the appearance of symptoms of EAE had no protective effect. These results indicate that at least some CD4(+) T cells have a regulatory function that prevent the activation of self-reactive T cells...
  72. pmc Experimental autoimmune encephalomyelitis in mice expressing the autoantigen MBP 1-10 covalently bound to the MHC class II molecule I-Au
    Florian C Kurschus
    Department of Neuroimmunology, Max Planck Institute of Neurobiology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Int Immunol 18:151-62. 2006
    ..Our data show that in the circumstances where autoantigenic priming persists, and where the number of antigen-specific T cells is high enough, autoimmunity may prevail over very potent tolerance-inducing mechanisms...
  73. ncbi Experimental cerebral hypoperfusion induces white matter injury and microglial activation in the rat brain
    Eszter Farkas
    Department of Molecular Neurobiology, University of Groningen, The Netherlands
    Acta Neuropathol 108:57-64. 2004
    ..Since the detected glial changes correspond with those reported in vascular and Alzheimer dementia, this model of cerebral hypoperfusion may serve to characterize the causal relationship between ischemia and white matter damage...
  74. pmc The role of citrullinated proteins suggests a novel mechanism in the pathogenesis of multiple sclerosis
    Mario A Moscarello
    Department of Structural Biology and Biochemistry, Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, ON, Canada
    Neurochem Res 32:251-6. 2007
    ..We propose that citrullinated MBP, resulting from elevated levels of PAD 2 represents an important biochemical pathway in the pathogenesis of MS...
  75. ncbi Protective effects of a peroxisome proliferator-activated receptor-beta/delta agonist in experimental autoimmune encephalomyelitis
    Paul E Polak
    Department of Anesthesiology, University of Illinois, and Jesse Brown Veteran s Affairs Research Division, Chicago, IL 60612, USA
    J Neuroimmunol 168:65-75. 2005
    ..RTPCR analysis showed that GW0742 increased expression of some myelin genes. These data demonstrate that PPARdelta agonists, like other PPAR ligands, can exert protective actions in an autoimmune model of demyelinating disease...
  76. ncbi Macrophage-depletion induced impairment of experimental CNS remyelination is associated with a reduced oligodendrocyte progenitor cell response and altered growth factor expression
    Mark R Kotter
    Cambridge Centre for Brain Repair, and Neuroregeneration Laboratory, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    Neurobiol Dis 18:166-75. 2005
    ..These data suggest that the macrophage response to toxin-induced demyelination influences the growth factor environment, thereby affecting the behavior of OPCs and hence the efficiency of remyelination...
  77. ncbi Expansion and functional relevance of high-avidity myelin-specific CD4+ T cells in multiple sclerosis
    Bibiana Bielekova
    Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:3893-904. 2004
    ..These data have important implications for autoimmunity research and should be considered in the development of Ag-specific therapies in MS...
  78. ncbi High incidence of spontaneous autoimmune encephalomyelitis in immunodeficient anti-myelin basic protein T cell receptor transgenic mice
    J J Lafaille
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Cell 78:399-408. 1994
    ..The data also suggest that spontaneous EAE may be triggered by an in situ activation of CD4+ anti-MBP cells in the nervous system...
  79. pmc Thyroid hormone administration enhances remyelination in chronic demyelinating inflammatory disease
    Mercedes Fernandez
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy
    Proc Natl Acad Sci U S A 101:16363-8. 2004
    ..Moreover, thyroid hormone exerts a neuroprotective effect with respect to axonal pathology...
  80. pmc Deimination of membrane-bound myelin basic protein in multiple sclerosis exposes an immunodominant epitope
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Proc Natl Acad Sci U S A 103:4422-7. 2006
    ..These findings suggest a mechanism for initial loss of myelin stability and the autoimmune pathogenesis of multiple sclerosis...
  81. ncbi A combinatorial network of evolutionarily conserved myelin basic protein regulatory sequences confers distinct glial-specific phenotypes
    Hooman F Farhadi
    Royal Victoria Hospital, Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec H3A 1A1, Canada
    J Neurosci 23:10214-23. 2003
    ....
  82. ncbi A tale of two citrullines--structural and functional aspects of myelin basic protein deimination in health and disease
    George Harauz
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1
    Neurochem Res 32:137-58. 2007
    ..In this review, we describe the structural and functional consequences of MBP deimination in healthy and diseased myelin...
  83. ncbi Effect of arginine loss in myelin basic protein, as occurs in its deiminated charge isoform, on mediation of actin polymerization and actin binding to a lipid membrane in vitro
    Joan M Boggs
    Division of Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
    Biochemistry 44:3524-34. 2005
    ..Thus, this posttranslational modification of MBP, which occurs early in life and is increased in multiple sclerosis, attenuates the ability of MBP to polymerize and bundle actin, and to bind it to a negatively charged membrane...
  84. ncbi Transfer of central nervous system autoantigens and presentation in secondary lymphoid organs
    Alex F de Vos
    Department of Immunology, Erasmus Medical Centre, 3000 DR Rotterdam, The Netherlands
    J Immunol 169:5415-23. 2002
    ..A major implication is that novel therapeutic strategies may be targeted to peripheral events, thereby circumventing the blood-brain barrier...
  85. ncbi Age-induced decrease of glycoprotein Po and myelin basic protein gene expression in the rat sciatic nerve. Repair by steroid derivatives
    R C Melcangi
    Department of Endocrinology, University of Milan, Italy
    Neuroscience 85:569-78. 1998
    ....
  86. ncbi Study of expression of myelin basic proteins (MBPs) in developing rat brain using a novel antibody reacting with four major isoforms of MBP
    Kyoichi Akiyama
    Department of Bioactivity Regulation, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan
    J Neurosci Res 68:19-28. 2002
    b>Myelin basic proteins (MBPs) are the major protein components of myelin. MBP isoforms are known to have different expression patterns...
  87. ncbi Analyses of proteolipid protein mutants show levels of proteolipid protein regulate oligodendrocyte number and cell death in vitro and in vivo
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit MI 48201, USA
    Neurochem Res 29:2095-103. 2004
    ..The regulation of apoptosis by PLP gene expression occurs independently of myelination, indicating that the PLP gene has multiple primary functions...
  88. ncbi Membrane-anchoring and charge effects in the interaction of myelin basic protein with lipid bilayers studied by site-directed spin labeling
    Ian R Bates
    Department of Molecular Biology and Genetics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 278:29041-7. 2003
    ..The mobility at F42A/F43A and especially F86A/F87A increased significantly. Depth measurements and helical wheel analysis indicated that the Phe-86/Phe-87 region could form a surface-seeking amphipathic alpha-helix...
  89. ncbi Major T-cell responses in multiple sclerosis
    L Steinman
    Department of Neurologic Sciences, Beckman Center for Molecular and Genetic Medicine, Stanford University, Stanford, CA 94305, USA
    Mol Med Today 1:79-83. 1995
    ..If this region of MBP is critical in the pathogenesis of MS, then therapy aimed at controlling the immune response to this immunodominant region of MBP may be beneficial in treating MS...
  90. ncbi Design of novel cyclic altered peptide ligands of myelin basic protein MBP83-99 that modulate immune responses in SJL/J mice
    Maria Katsara
    Immunology and Vaccine Laboratory, Burnet Institute Austin Campus, Studley Road, Heidelberg, 3084 Victoria, Australia
    J Med Chem 51:3971-8. 2008
    ..In particular, cyclo(83-99)[A (91)]MBP 83-99 is a promising mutant peptide analogue for the potential treatment of MS...
  91. ncbi Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture
    Samuel J Jackson
    Department of Neuroinflammation, Institute of Neurology, University College London, 1 Wakefield Street, London WC1N 1PJ, UK
    Eur J Neurosci 20:2267-75. 2004
    ....
  92. ncbi Golli protein negatively regulates store depletion-induced calcium influx in T cells
    Ji Ming Feng
    Semel Institute of Neuroscience and Human Behavior, UCLA Geffen School of Medicine, 635 Charles Young Drive, Los Angeles, California 90095, USA
    Immunity 24:717-27. 2006
    ..These results indicate that golli functions in a unique way to regulate T cell activation through a mechanism involving the modulation of the calcium homeostasis...
  93. pmc Interaction forces and adhesion of supported myelin lipid bilayers modulated by myelin basic protein
    Younjin Min
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 106:3154-9. 2009
    ..Thus loss of adhesion, possibly contributing to demyelination, can be brought about by either an excess or deficit of MBP or anionic lipids...
  94. ncbi The golli-myelin basic protein negatively regulates signal transduction in T lymphocytes
    Ji Ming Feng
    UCLA Medical School, Neuropsychiatric Institute, Room 47 448, 760 Westwood Plaza, Los Angeles, CA 90024 17519, USA
    J Neuroimmunol 152:57-66. 2004
    ..Structure-function assays indicate that BG21 inhibitory activity resides in the golli domain rather than in MBP domain of the molecule. These results reveal a novel role for MBP gene products in T lymphocytes within the immune system...
  95. ncbi Differences in susceptibility of MBP charge isomers to digestion by stromelysin-1 (MMP-3) and release of an immunodominant epitope
    Cheryl A D'Souza
    Centre for Research in Neurodegenerative Diseases, University of Toronto, 6 Queen s Park Cres West, Toronto, M5S 3H2, Canada
    Neurochem Res 31:1045-54. 2006
    ..Stromelysin-1 was able to cleave myelin basic protein in the presence of lipids and within the context of myelin and released several peptides including peptides containing the immunodominant epitope...
  96. ncbi Cytoskeleton in myelin-basic-protein-deficient shiverer oligodendrocytes
    C A Dyer
    Department of Biomedical Sciences, E K Shriver Center, Waltham, Mass 02254, USA
    Dev Neurosci 17:53-62. 1995
    ..In the absence of this signal, oligodendrocytes apparently do not normally assemble cytoskeleton; this may be one important basis for the abnormal morphology of intact shiverer CNS...
  97. ncbi Structural properties of proteins specific to the myelin sheath
    P Kursula
    Department of Biochemistry, University of Oulu, Oulu, Finland
    Amino Acids 34:175-85. 2008
    ..In this review, the structural properties of a number of myelin-specific proteins are described...
  98. ncbi Antibodies to neuron-specific antigens in children with autism: possible cross-reaction with encephalitogenic proteins from milk, Chlamydia pneumoniae and Streptococcus group A
    A Vojdani
    Section of Neuroimmunology, Immunosciences Laboratory, Inc, 8693 Wilshire Boulevard, Suite 200, Beverly Hills, CA 90211, USA
    J Neuroimmunol 129:168-77. 2002
    ..These results suggest a mechanism by which bacterial infections and milk antigens may modulate autoimmune responses in autism...
  99. ncbi Region-specific myelin pathology in mice lacking the golli products of the myelin basic protein gene
    Erin C Jacobs
    Neuropsychiatric Institute, University of California Los Angeles School of Medicine, Los Angeles, California 90024, USA
    J Neurosci 25:7004-13. 2005
    ..Together, all these findings suggest a role for golli proteins in oligodendrocyte differentiation, migration, and/or myelin elaboration in the brain...
  100. ncbi Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3':5'-monophosphate-dependent protein kinase
    A Kishimoto
    J Biol Chem 260:12492-9. 1985
    ..These results provide some clues to understanding the rationale that these kinases may show different but sometimes similar functions depending on the structure of target phosphate acceptor proteins...
  101. ncbi Tumor necrosis factor-alpha and alphaB-crystallin up-regulation during antibody-mediated demyelination in vitro: a putative protective mechanism in oligodendrocytes
    Cécile Besson Duvanel
    Neurochemistry Laboratory, Department of Pediatrics, CHUV, Lausanne, Switzerland
    J Neurosci Res 78:711-22. 2004
    ..These results suggest that TNF-alpha, often regarded as a promoter of oligodendroglial death, could alternatively mediate a protective pathway through alphaB-crystallin up-regulation...

Research Grants74

  1. Early intervention to restore OL and myelin development in leukodystrophy model
    Jean de Vellis; Fiscal Year: 2009
    ..Our preliminary work has shown that the ASPA knockout OLs exhibit lack of mature myelin basic proteins and lack of immunostaining of myelin proteins in cell processes in the CNS, even though presence of ..
  2. EXPRESSION OF THE MYELIN BASIC PROTEIN GENE
    CARMIE PUCKETT; Fiscal Year: 1991
    ..the following purposes: (1) to identify those cis elements involved in the high level expression of the myelin basic proteins in the oligodendrocyte; (2) to examine which tissues and cell types possess the trans factors necessary ..
  3. FUNCTIONAL DOMAINS OF THE MYELIN BASIC PROTEINS
    Emily Friedman; Fiscal Year: 1991
    A complete understanding of the role the myelin basic proteins (MBPs) play in organizing the myelin membrane will elucidate important mechanisms underlying myelin repair...
  4. Recombinant T-cell Receptor Ligands for Treatment of Uveitis
    Grazyna Adamus; Fiscal Year: 2006
    ..To determine an effective treatment regimen for clinical rat AAU using RTLs. 2. To determine the abilities of RTLs to inhibit recurrent anterior and posterior uveitis in Lewis rats. [unreadable] [unreadable] [unreadable] [unreadable]..
  5. IMMUNOLOGICAL MECHANISM OF INFLAMMATION IN UVEITIS & EAE
    Grazyna Adamus; Fiscal Year: 2001
    ..3) Examine the role of cytokines and chemokines in the recruitment of cell to the eye in primary and recurrent uveitis associated with EAE after immunization of Lewis rats with MBP. ..
  6. MECHANISM OF RETINAL DAMAGE IN AUTOIMMUNE RETINOPATHY
    Grazyna Adamus; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  7. Anti-idiotypic immune regulation by T cell vaccination
    Jingwu Zhang; Fiscal Year: 2004
    ..The long-term goal of the study is to define the mechanism of T cell vaccination and develop a practical peptide-based vaccination approach. ..
  8. MYELIN BASIC PROTEIN, TOLERANCE AND AUTOIMMUNE DISEASE
    Joan Goverman; Fiscal Year: 2004
    ..They will investigate mechanisms of tolerance that operate on these CTLs as well as their effector mechanisms and target cells within the CNS. ..
  9. Sex Chromosome Effects in Lupus
    Rhonda Voskuhl; Fiscal Year: 2008
    ..This disease was chosen in light of the 9:1 female preponderance of the human disease system lupus erythematosus. [unreadable] [unreadable] [unreadable]..
  10. NOVEL MHC CLASS II CONSTRUCTS FOR TREATMENTS OF CBD
    GREGORY BURROWS; Fiscal Year: 2003
    ....
  11. The Effect of Sex Chromosomes on Immune Responses
    Rhonda Voskuhl; Fiscal Year: 2009
    ..Together these proposed studies will greatly advance the understanding of the role of sex chromosomes on immune responses. ..
  12. Events Leading to Loss Of Tolerance to Myelin Basic Pro*
    Joan Goverman; Fiscal Year: 2003
    ..We will utilize this unique experimental system in combination with several transgenic, knock-out and knock-in mouse models bred onto the appropriate genetic background to test this hypothesis. ..
  13. Regulatory T cells in MBP-Specific Autoimmunity
    Joan Goverman; Fiscal Year: 2006
    ..Understanding how regulatory T cells prevent MBP-specific T cells from mediating disease is important for understanding the pathogenesis of MS and may provide insights into new therapeutic strategies. ..
  14. HLA-DQ-derived RTLs for Treatment of Celiac Disease
    GREGORY BURROWS; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  15. NOVEL MHC CLASS II CONSTRUCTS FOR TREATMENT OF EAE
    GREGORY GEORGE BURROWS; Fiscal Year: 2010
    ..Evaluation of the in vivo effects RTLs have on relapsing-remitting and chronic models of EAE. The work proposed will provide a solid base for pharmacological intervention in CD4+ T cell mediated autoimmune diseases. ..
  16. Mechanisms of Disease Progression in EAE
    Richard Jones; Fiscal Year: 2007
    ..Aim 3 will evaluate the number and phenotype of regulatory T cells capable of blocking or reducing disease. [unreadable] [unreadable]..
  17. THE ROLE OF LYMPHOCYTES IN CEREBRAL ISCHEMIC INJURY
    Kyra Becker; Fiscal Year: 2004
    ....
  18. Human MHC Class II Constructs For Autoimmume Therapy
    GREGORY BURROWS; Fiscal Year: 2005
    ..Understanding how these unique, rationally engineered drugs work will provide a solid foundation for pharmacological intervention in CD4+ T cell mediated autoimmune diseases. ..
  19. Protective Effect of Pregnancy Doses of Estrogens in EAE
    Rhonda Voskuhl; Fiscal Year: 2005
    ..abstract_text> ..
  20. CNS antigen sensitization in stroke.
    Kyra Becker; Fiscal Year: 2009
    ..Data derived from this study will be used to plan future trials of immunomodulation in patients with stroke. ..
  21. Large Scale Images of Gene Transcription in MS and EAE
    Lawrence Steinman; Fiscal Year: 2004
    ..The discovery of the role of osteopontin, first identified in large scale profiles, exemplifies how this approach may help us to understand MS and to develop new therapies. ..
  22. Mimic Peptides of HIV-1 Epitopes as Vaccines
    Darcy Wilson; Fiscal Year: 2005
    ..Ultimately, this peptide sequence information will be incorporated into a therapeutic/ prophylactic multi-epitope vaccine formulation based on multiple peptides, fusion proteins or DNA sequences in viral vectors. ..
  23. CD8+ T Cells Control Autoimmunity In EAE
    Hong Jiang; Fiscal Year: 2007
    ..Thus, the studies will provide theoretical basis for clinical application of this regulatory pathway in prevention and treatment of multiple sclerosis in man. [unreadable] [unreadable] [unreadable]..
  24. GENETIC ELEMENTS REGULATING MYELIN GENE EXPRESSION
    Anthony Campagnoni; Fiscal Year: 2001
    DESCRIPTION: The Golli-mbp gene encodes two families of proteins, the Golli proteins and the myelin basic proteins. The overall goal of this application is to identify the genetic regulatory elements that govern specificity in the ..
  25. IN VIVO TREATMENT OF EAE WITH ANTI I-A ANTIBODIES
    Lawrence Steinman; Fiscal Year: 2001
    ..Aim 5 is to assess the possibility of using DNA vaccination against TCR Vb for treatment of EAE. It is hoped that these studies will provide the basis for future clinical trials in MS patients. ..
  26. Emerging Strategies: bridging basic and clinical science
    Robin Miskimins; Fiscal Year: 2008
    ..Ample discussion periods and an open forum panel discussion involving the invited speakers will allow for extensive interaction between participants. [unreadable] [unreadable] [unreadable] [unreadable]..
  27. Perinatal hypoxia-ischemia, glutamate receptors, and Nitric Oxide Synthase
    Zhengwei Cai; Fiscal Year: 2007
    ..Direct effects of inflammatory cytokines and IGF-1 on survival and differentiation of OLs will be examined in cultures of OLs at various maturation stages. ..
  28. Signaling mechanisms in oligodendrocytes
    Robin Miskimins; Fiscal Year: 2006
    ..Completion of the studies described will provide significant insight into the role of p85 in regulating oligodendrocyte differentiation. [unreadable] [unreadable]..
  29. Trigeminal Neuropathic Pain Mechanisms
    Michael Henry; Fiscal Year: 2006
    ..Identification of specific sodium channel alterations after injury may help develop more selective treatments for neuropathic pain. ..
  30. EFFECTOR MOLECULES IN DEMYELINATING DISEASE
    Anne Cross; Fiscal Year: 2001
    ..Since there now have been identified pharmaceutic agents which can inhibit the production of PN, our findings from these studies may indicate an area with therapeutic potential for MS. ..
  31. Golli-MBP Gene Expression in the Nervous System
    Anthony Campagnoni; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  32. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  33. Sodium Channel Expression in Human Teeth
    Michael Henry; Fiscal Year: 2009
    ..abstract_text> ..
  34. CD8+ T CELL RECOGNITION OF TH1 AND TH2
    Hong Jiang; Fiscal Year: 2003
    ..We will determine if this difference uniquely applies to the recognition of TCR peptide presented by Qa-1 or if CD8+ T cells specific for other peptides presented by MHC class I molecules also distinguish between TH1 and TH2 targets. ..
  35. A PHASE ll TRIAL OF RITUXAN IN MULTIPLE SCLEROSIS
    DOROTHY CROSS; Fiscal Year: 2007
    ..Parks and Naismith, and PI will be able to focus 30% more time on investigator-initiated trials and mentoring junior colleagues. ..
  36. THE ROLE OF B CELLS IN EAE PATHOGENESIS
    Anne Cross; Fiscal Year: 2004
    ..Methods to alter in humans the number and function of B cells and Abs already exist, making our results potentially applicable to MS therapy. ..
  37. PEPTIDE MEDIATED IMMUNOTHERAPY FOR AUTOIMMUNE DISEASE
    Lawrence Steinman; Fiscal Year: 2003
    ..This work with APL may be applicable not only to MS, but to other autoimmune diseases including juvenile diabetes, rheumatoid arthritis, and inflammatory bowel disease. ..
  38. Perinatal infection, cytokines and brain injury
    Zhengwei Cai; Fiscal Year: 2008
    ....
  39. Synthesis and Assembly of Basic Proteins into Myelin.
    Anthony Campagnoni; Fiscal Year: 2009
    ..These studies are relevant to demyelinating diseases, like MS and leukodystrophies, and will enhance our understanding of myelination, remyelination and the effects of hypomyelination on neural function. ..
  40. EFFERENT INNERVATION OF IDENTIFIED VESTIBULAR AFFERENTS
    Anna Lysakowski; Fiscal Year: 2008
    ..The intent is to produce a body of morphological, neurochemical and cell biological knowledge from which physiologically and pharmacologically testable hypotheses can be derived. [unreadable] [unreadable] [unreadable]..
  41. Proteolytic Mechanisms of Axonal and Dendritic Damage
    Ming Liu; Fiscal Year: 2008
    ..abstract_text> ..
  42. REGULATION OF INTERFERON ACTIVITY BY SHP-1 IN THE CNS
    PAUL MASSA; Fiscal Year: 2007
    ..We believe that information gained under these specific aims will elucidate mechanisms whereby SHP-1 controls virus infections in oligodendrocytes and perhaps other cells of the CNS. ..
  43. Effects of intracellular angiotensin II
    Julia Cook; Fiscal Year: 2009
    ..abstract_text> ..
  44. Effect of Macrolide Treatment on a Novel Pathway of Neutrophilic Inflammation in
    J Edwin Blalock; Fiscal Year: 2010
    ..If successful, the current proposal will yield fundamental insights into the disease process and a new and specific biomarker for COPD as well as suggesting new therapeutic targets. (End of Abstract) ..
  45. Canavan Disease: Pathogenesis and Treatment
    Aryan Namboodiri; Fiscal Year: 2008
    ..These studies are likely to lead to a simple and inexpensive method for the treatment of CD and also advance our knowledge on the functional roles of NAA and the relationship of increased NAA to CD pathology. ..
  46. Migration Inhibitory Factor in the Progression of EAE
    Caroline C Whitacre; Fiscal Year: 2010
    ..This project studies a key factor involved in disease progression and will identify specific points in disease when therapeutic intervention is likely to be successful. ..
  47. IMMUNOTHERAPY OF MYASTHENIA GRAVIS
    J Blalock; Fiscal Year: 2001
    ..Is there restricted usage of TCR by AChR 100-116 specific T-cells recognized by CTCR8? 6. Can EAMG-associated T-cells of other specificities be identified with complementary peptides to different AChR T-cell epitopes? ..
  48. MAP KINASE REGULATION OF MICROGLIAL ACTIVATION
    Narayan Bhat; Fiscal Year: 2004
    ..The suppressive effects of the kinase inhibitors on activation-associated microglial functions; i.e., targeting of oligodendrocytes and myelin phagocytosis will be tested using in vitro models. ..
  49. Vaccine therapy of congenital adrenal Hyperplasia
    Scott Rivkees; Fiscal Year: 2003
    ..We also anticipate being able to extend this novel approach to the treatment of other receptor-mediated endocrine disorders, such as hyperthyroidism. ..
  50. PDGF SIGNALING IN OLIGODENDROCYTE DEVELOPMENT
    RANDALL MCKINNON; Fiscal Year: 2004
    ..abstract_text> ..
  51. A-1 Adenosine Receptor Action in Developing Heart
    Scott Rivkees; Fiscal Year: 2005
    ..We anticipate that these studies will identify Rho and ROCK as critical signaling molecules in heart development and reveal that cardiac GTPase activity transduces the effects of adenosine. ..
  52. Cellular Mechanisms of Perinatal White Matter Injury
    STEPHEN BACK; Fiscal Year: 2005
    ..Through this work we will gain fundamental new insights into the cellular and molecular mechanisms that underlie neonatal white matter injury and, thereby, develop new strategies to prevent PVL. ..
  53. CANCER SUSCEPTIBILITY IN AN ANIMAL MODEL
    Raymond Yeung; Fiscal Year: 2004
    ..Appreciation of these 'modifier' genes will impart new knowledge about the interplay between genes and environment during the development of complex diseases such as cancer. ..
  54. Evaluation of MicroRNA Expression in CD4 T cells in Type 1 Diabetes
    Alberto Pugliese; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  55. CB1 Receptor Action on the Developing Hippocampus
    Scott Rivkees; Fiscal Year: 2005
    ..We anticipate that these studies will result in new insights into the potential effects of cannabinoid action on the developing brain. ..
  56. A1-ADENOSINE RECEPTOR ACTION IN HIPPOCAMPUS
    Scott Rivkees; Fiscal Year: 2003
    ..We anticipate these studies will lead to new insights into the mechanisms of A1AR action. We also anticipate that these studies will identify A1ARs as an important regulator of axon growth. ..
  57. Cellular Mechanisms of Preterm White Matter Injury
    STEPHEN BACK; Fiscal Year: 2003
    ....
  58. Development of Protein-Detecting Microarrays
    Thomas Kodadek; Fiscal Year: 2002
    ..If successful, we will then move to apply these technologies to the creation and evaluation of functional PDMs. ..
  59. Latent Mobility Abnormalities and Disability
    Joe Verghese; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  60. Pharmacogenetics in Tourette Syndrom
    Harvey Singer; Fiscal Year: 2004
    ..The identification of genetic polymorphisms that are associated with clinical disease or treatment success, has obvious benefits. ..
  61. MR Image Analysis in MS: Identification of a Surrogate
    Ponnada Narayana; Fiscal Year: 2002
    ..While the main emphasis is on MS, this system should be readily adaptable to investigate and manage various neurological disorders that require accurate determination of tissue volumes and their temporal change. ..
  62. DNA Tolerizing Vaccine Therapy for Multiple Sclerosis
    Hideki Garren; Fiscal Year: 2002
    ..S. FDA for the evaluation of a DNA tolerizing vaccine in a phase I clinical trial. ..
  63. 3 T Whole Body Magnetic Resonance Scanner
    Ponnada Narayana; Fiscal Year: 2004
    ..Strong institutional commitment and financial plans for operating the scanner, particularly the long-term maintenance, are described. ..
  64. MAGNETIC RESONANCE OF SPINAL CORD INJURY
    Ponnada Narayana; Fiscal Year: 2006
    ..With recent advances in the treatment of SCI, there is a critical need to noninvasively monitor the efficacy of a given treatment. The proposed MRI and fMRI studies greatly aid realize this goal. ..
  65. Nestin: A Putative Marker of a Mammary Stem and Progenitor Cell Lineage
    Teresa Wood; Fiscal Year: 2007
    ..This novel marker may identify a population of undifferentiated breast tumor stem or progenitor cells. [unreadable] [unreadable] [unreadable]..
  66. OLIGODENDROCYTE GENERATION--A MULTIFACTORIAL APPROACH
    Teresa Wood; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  67. ADENOSINE AND TRAUMATIC BRAIN INJURY
    Patrick Kochanek; Fiscal Year: 2007
    ..These studies explore the most promising adenosine-based therapy for TBI-A2a receptor antagonists. Our bench to bedside track record ensures translation to the clinic. [unreadable] [unreadable]..
  68. Neuroinflammation in Cholesterol-Induced AD Pathogenesis
    Narayan Bhat; Fiscal Year: 2009
    ..The outcome of these studies should have implications for developing novel and effective anti- inflammatory treatments for AD. ..
  69. MRI of Angiogenesis in Spinal Cord Injury
    Ponnada Narayana; Fiscal Year: 2009
    ..In addition, it is possible to modulate angiogenesis in conjunction with other treatments, such as cellular grafts to further enhance the recovery from SCI. Thus the proposed studies have a very high degree of clinical relevance. ..
  70. SERIAL MAGNETIC RESONANCE IN MULTIPLE SCLEROSIS
    Ponnada Narayana; Fiscal Year: 2002
    ..A further significance of these studies is that they may enable us to follow objectively and quantitatively the efficacy of drugs in the treatment of MS. ..
  71. MAP KINASES IN OLIGODENDROCYTE CELL SIGNALING
    Narayan Bhat; Fiscal Year: 2005
    ..Putative MAPK-responsive elements and relevant binding activities will be determined by deletion and gel shift analyses ..
  72. New Chemistry for Analysis of Multi-protein complexes
    Thomas Kodadek; Fiscal Year: 2005
    ..Finally, a new aspect application of this oxidative chemistry is proposed: the development of a general method by which to identify the protein target(s) of bioactive small molecules. ..
  73. NO DAMAGE TO FOLATE CYCLE IN THE CENTRAL NERVOUS SYSTEM
    Kenneth Hensley; Fiscal Year: 2005
    ..abstract_text> ..