connexin 43

Summary

Summary: A 43-kDa peptide which is a member of the connexin family of gap junction proteins. Connexin 43 is a product of a gene in the alpha class of connexin genes (the alpha-1 gene). It was first isolated from mammalian heart, but is widespread in the body including the brain.

Top Publications

  1. ncbi Cardiac malformation in neonatal mice lacking connexin43
    A G Reaume
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Science 267:1831-4. 1995
  2. pmc Connexin43 phosphorylation: structural changes and biological effects
    Joell L Solan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800 Box 19024, Seattle, WA 98109, USA
    Biochem J 419:261-72. 2009
  3. pmc Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture
    Jorge E Contreras
    Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago 340, Chile
    Proc Natl Acad Sci U S A 99:495-500. 2002
  4. pmc Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
    Juan A Orellana
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Alameda 340, Santiago, Chile
    Glia 58:329-43. 2010
  5. pmc Connexin 43 controls the multipolar phase of neuronal migration to the cerebral cortex
    Xiuxin Liu
    Department of Neurobiology and Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 109:8280-5. 2012
  6. ncbi Impairment of diazoxide-induced formation of reactive oxygen species and loss of cardioprotection in connexin 43 deficient mice
    Frank R Heinzel
    Institut fur Pathophysiologie, Universitatsklinikum Essen, Germany
    Circ Res 97:583-6. 2005
  7. pmc Pannexin 1: the molecular substrate of astrocyte "hemichannels"
    Rodolfo Iglesias
    The Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 29:7092-7. 2009
  8. pmc Connexin 43 hemichannels are permeable to ATP
    Jian Kang
    Department of Cell Biology and Anatomy, New York Medical College, Valhalla, New York 10595, USA
    J Neurosci 28:4702-11. 2008
  9. pmc Actin cytoskeleton rest stops regulate anterograde traffic of connexin 43 vesicles to the plasma membrane
    James W Smyth
    University of California San Francisco, Cardiovascular Research Institute, 555 Mission Bay Boulevard South, San Francisco, CA 94158, USA
    Circ Res 110:978-89. 2012
  10. pmc Reduced connexin 43 expression and its effect on the development of vascular lesions in retinas of diabetic mice
    Michael W Bobbie
    Departments of Medicine, University of Arizona, Tucson, Arizona, USA
    Invest Ophthalmol Vis Sci 51:3758-63. 2010

Research Grants

  1. Structure/Function Analysis of Low pl Connexin Isoforms
    ELLIOT HERTZBERG; Fiscal Year: 2003
  2. Microvascular Dysfunction in Hyperhomocysteinemia
    Shawn E Bearden; Fiscal Year: 2012
  3. Cx43 phosphorylation modulates Kras mediated pancreas cancer progression
    Paul D Lampe; Fiscal Year: 2012
  4. Mechanisms of Ventricular Tachycardia in Lipotoxic Cardiomyopathy
    JOHN PEARCE MORROW; Fiscal Year: 2013
  5. PROTEIN KINASE C IN THE LENS
    DOLORES JEAN TAKEMOTO; Fiscal Year: 2010
  6. Connexin 43 in Bone Remodeling
    Roberto Civitelli; Fiscal Year: 2013
  7. GENETIC STUDIES OF ODDD
    Ethylin Wang Jabs; Fiscal Year: 2010
  8. Implications of Endothelial-Myocyte Uncoupling in Cardiac Arrhythmia
    Suresh C Tyagi; Fiscal Year: 2010
  9. Satellite Glial Cells
    Luc Jasmin; Fiscal Year: 2013
  10. Renin angiotensin system and connexin43
    Samuel C Dudley; Fiscal Year: 2013

Detail Information

Publications336 found, 100 shown here

  1. ncbi Cardiac malformation in neonatal mice lacking connexin43
    A G Reaume
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Science 267:1831-4. 1995
    ..This finding suggests that Cx43 plays an essential role in heart development but that there is functional compensation among connexins in other parts of the developing fetus...
  2. pmc Connexin43 phosphorylation: structural changes and biological effects
    Joell L Solan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800 Box 19024, Seattle, WA 98109, USA
    Biochem J 419:261-72. 2009
    ..The present review assesses our current understanding of the effects of phosphorylation on connexin43 structure and function that in turn regulate gap junction biology, with an emphasis on events occurring in heart and skin...
  3. pmc Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture
    Jorge E Contreras
    Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago 340, Chile
    Proc Natl Acad Sci U S A 99:495-500. 2002
    ..Alternatively, dying astrocytes might compromise the health of neighboring cells via Cx43 gap junctions, thereby promoting the propagation of cell death...
  4. pmc Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
    Juan A Orellana
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Alameda 340, Santiago, Chile
    Glia 58:329-43. 2010
    ..glucose during the hypoxia in a concentration-dependent manner (and by zero glucose) and were not observed in connexin 43(-/-) astrocytes...
  5. pmc Connexin 43 controls the multipolar phase of neuronal migration to the cerebral cortex
    Xiuxin Liu
    Department of Neurobiology and Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 109:8280-5. 2012
    ..from the bipolar to the mutipolar and then back to bipolar again, is associated with expression of connexin 43 (Cx43) and, that knockdown of Cx43 retards, whereas its overexpression enhances, this morphogenetic process...
  6. ncbi Impairment of diazoxide-induced formation of reactive oxygen species and loss of cardioprotection in connexin 43 deficient mice
    Frank R Heinzel
    Institut fur Pathophysiologie, Universitatsklinikum Essen, Germany
    Circ Res 97:583-6. 2005
    Protection by ischemic preconditioning is lost in cardiomyocytes and hearts of heterozygous connexin 43 deficient (Cx43+/-) mice...
  7. pmc Pannexin 1: the molecular substrate of astrocyte "hemichannels"
    Rodolfo Iglesias
    The Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 29:7092-7. 2009
    ..Moreover, quantification of the amount of ATP released from wild-type, Cx43-null, and Panx1-KD astrocytes indicates that downregulation of Panx1, but not of Cx43, prevented ATP release from these cells...
  8. pmc Connexin 43 hemichannels are permeable to ATP
    Jian Kang
    Department of Cell Biology and Anatomy, New York Medical College, Valhalla, New York 10595, USA
    J Neurosci 28:4702-11. 2008
    ..Previous studies have implicated connexin 43 (Cx43) in ATP release, but definitive proof that ATP exits through Cx43 hemichannels does not exist...
  9. pmc Actin cytoskeleton rest stops regulate anterograde traffic of connexin 43 vesicles to the plasma membrane
    James W Smyth
    University of California San Francisco, Cardiovascular Research Institute, 555 Mission Bay Boulevard South, San Francisco, CA 94158, USA
    Circ Res 110:978-89. 2012
    The intracellular trafficking of connexin 43 (Cx43) hemichannels presents opportunities to regulate cardiomyocyte gap junction coupling...
  10. pmc Reduced connexin 43 expression and its effect on the development of vascular lesions in retinas of diabetic mice
    Michael W Bobbie
    Departments of Medicine, University of Arizona, Tucson, Arizona, USA
    Invest Ophthalmol Vis Sci 51:3758-63. 2010
    PURPOSE. To examine whether diabetes-induced connexin 43 downregulation promotes retinal vascular lesions characteristic of diabetic retinopathy (DR). METHODS...
  11. pmc Glucose increases intracellular free Ca(2+) in tanycytes via ATP released through connexin 43 hemichannels
    Juan A Orellana
    Departamento de Fisiologia, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Glia 60:53-68. 2012
    ..Glucose transportation, primarily via glucose transporters, and metabolism via anaerobic glycolysis increased connexin 43 (Cx43) hemichannel activity, evaluated by ethidium uptake and whole cell patch clamp recordings, through a K(..
  12. pmc Osteoblast connexin43 modulates skeletal architecture by regulating both arms of bone remodeling
    Marcus Watkins
    Department of Internal Medicine and Cell Biology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 22:1240-51. 2011
    ..Thus Cx43 in osteogenic cells is a critical regulator of both arms of the bone remodeling cycle, its absence causing structural changes remindful of aged or disused bone...
  13. pmc Zonula occludens-1 alters connexin43 gap junction size and organization by influencing channel accretion
    Andrew W Hunter
    Department of Cell Biology and Anatomy, Cardiovascular Developmental Biology Center, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Biol Cell 16:5686-98. 2005
    ..We propose that ZO-1 controls the rate of Cx43 channel accretion at GJ peripheries, which, in conjunction with the rate of GJ turnover, regulates GJ size and distribution...
  14. ncbi Connexin 43 hemichannels contribute to the propagation of apoptotic cell death in a rat C6 glioma cell model
    E Decrock
    Department of Basic Medical Sciences Physiology group, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Cell Death Differ 16:151-63. 2009
    ..Buffering cytoplasmic Ca(2+) changes inhibited the spread of apoptosis in both cases. We conclude that Cx43 hemichannels, in concert with their GJ counterparts, play a role in communicating cytC-induced apoptotic cell death messages...
  15. ncbi Cx43 hemichannels and gap junction channels in astrocytes are regulated oppositely by proinflammatory cytokines released from activated microglia
    Mauricio A Retamal
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Santiago 6513492, Chile
    J Neurosci 27:13781-92. 2007
    ..Accordingly, this opposite regulation may affect glucose trafficking and certainly will modify the metabolic status of astrocytes involved in brain inflammation...
  16. pmc Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43
    D E Gutstein
    Section of Myocardial Biology, Cardiovascular Institute, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Circ Res 88:333-9. 2001
    ..1+/-0.13 versus 1.66+/-0.06; P:<0.01). This novel genetic murine model of primary sudden cardiac death defines gap junctional abnormalities as a key molecular feature of the arrhythmogenic substrate...
  17. pmc Autophagy: a pathway that contributes to connexin degradation
    Alexandra Lichtenstein
    Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA
    J Cell Sci 124:910-20. 2011
    ..These results demonstrate that autophagy can regulate cellular levels of wild-type connexins and imply that the persistence of accumulations of CX50P88S results from insufficient degradation capacity of constitutive autophagy...
  18. ncbi Transduction of cell survival signals by connexin-43 hemichannels
    Lilian I Plotkin
    Division of Endocrinology and Metabolism, the Center for Osteoporosis and Metabolic Bone Diseases, and the Central Arkansas Veterans Healthcare System, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Biol Chem 277:8648-57. 2002
    ....
  19. pmc Reciprocal regulation between proinflammatory cytokine-induced inducible NO synthase (iNOS) and connexin43 in bladder smooth muscle cells
    Kai Li
    Department of Molecular Signaling, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409 3898, Japan
    J Biol Chem 286:41552-62. 2011
    ..Our findings may provide an important molecular mechanism for the symptoms of bladder infection. In addition, it may further our understanding of the roles of GJs in inflammatory diseases...
  20. pmc Premature osteoblast clustering by enamel matrix proteins induces osteoblast differentiation through up-regulation of connexin 43 and N-cadherin
    Richard J Miron
    Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
    PLoS ONE 6:e23375. 2011
    ..evaluate the influence of cell clustering on vital osteoblast cell-cell communication and adhesion molecules, connexin 43 (cx43) and N-cadherin (N-cad) as assessed by immunofluorescence imaging, real-time PCR and Western blot ..
  21. ncbi Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein
    B J Warn-Cramer
    Department of Molecular Carcinogenesis, Cancer Research Center of Hawaii, School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA
    J Biol Chem 271:3779-86. 1996
    ....
  22. ncbi Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase
    B J Warn-Cramer
    Molecular Carcinogenesis Section, Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA
    J Biol Chem 273:9188-96. 1998
    ..Studies with connexin-43 mutants suggest that MAP kinase phosphorylation at one or more of the tandem Ser279/Ser282 sites is sufficient to disrupt gap junctional intercellular communication...
  23. ncbi The tumor-suppressive function of Connexin43 in keratinocytes is mediated in part via interaction with caveolin-1
    Stephanie Langlois
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada
    Cancer Res 70:4222-32. 2010
    ....
  24. ncbi Connexin 43 interacts with Bax to regulate apoptosis of pancreatic cancer through a gap junction-independent pathway
    Yan Sun
    Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai 200032, P R China
    Int J Oncol 41:941-8. 2012
    ..The results from the present study elucidate a novel mechanism of the Cx43-mediated regulation of apoptosis in pancreatic cancer...
  25. pmc Connexin 43 connexon to gap junction transition is regulated by zonula occludens-1
    J Matthew Rhett
    Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Biol Cell 22:1516-28. 2011
    b>Connexin 43 (Cx43) is a gap junction (GJ) protein widely expressed in mammalian tissues that mediates cell-to-cell coupling...
  26. pmc Expressing connexin 43 in breast cancer cells reduces their metastasis to lungs
    Zhongyong Li
    Division of Musculoskeletal Sciences, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Clin Exp Metastasis 25:893-901. 2008
    ..These results suggest that expressing Cx43 in breast cancer cells decreases their metastatic potential through a mechanism independent of gap junctional communication but, rather, related to N-cadherin expression and apoptosis...
  27. pmc ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannels
    Juan A Orellana
    Departamento de Fisiologia, Pontificia Universidad Catolica de Chile, Santiago, Chile
    J Neurochem 118:826-40. 2011
    ....
  28. ncbi Connexin 43 reverses malignant phenotypes of glioma stem cells by modulating E-cadherin
    Shi cang Yu
    Institute of Pathology and Southwest Cancer Center, Third Military Medical University, Chongqing, China
    Stem Cells 30:108-20. 2012
    ..Our results suggest that GSCs require the low expression of Cx43 for maintaining their malignant phenotype, and upregulation of Cx43 might be a potential strategy for treatment of malignant glioma...
  29. pmc Involvement of urinary bladder Connexin43 and the circadian clock in coordination of diurnal micturition rhythm
    Hiromitsu Negoro
    Department of Urology, Graduate School of Medicine, Kyoto University, Sakyo, Kyoto 606 8507, Japan
    Nat Commun 3:809. 2012
    ..Therefore, circadian oscillation of connexin43 is associated with the biological clock and contributes to diurnal changes in bladder capacity, which avoids disturbance of sleep by micturition...
  30. ncbi Role of connexin 43 in ischemic preconditioning does not involve intercellular communication through gap junctions
    X Li
    Institut fur Pathophysiologie, Zentrum fur Innere Medizin, Universitatsklinikum Essen, Hufelandstrasse 55, Essen 45122, Germany
    J Mol Cell Cardiol 36:161-3. 2004
    b>Connexin 43 (Cx 43) has recently been implicated in protection of ischemic preconditioning...
  31. ncbi Gradual loss of functional gap junction within progression of colorectal cancer -- a shift from membranous CX32 and CX43 expression to cytoplasmic pattern during colorectal carcinogenesis
    Luiza Kanczuga-Koda
    Department of Medical Pathomorphology, Medical University of Bialystok, Collegium Pathologicum, Waszyngtona St 13, 15 269 Bialystok, Poland
    In Vivo 24:101-7. 2010
    ....
  32. ncbi Connexin 43 expression and distribution in compensated and decompensated cardiac hypertrophy in patients with aortic stenosis
    Sawa Kostin
    Department of Experimental Cardiology, Max Planck Institute, Benekestr 2D 61231, Bad Nauheim, Germany
    Cardiovasc Res 62:426-36. 2004
    ..In advanced heart failure alterations of the major ventricular GJ protein, connexin 43 (Cx43) are found...
  33. ncbi Heart defects in connexin43-deficient mice
    J Ya
    Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, The Netherlands
    Circ Res 82:360-6. 1998
    ....
  34. ncbi Gap junction channels formed by coexpressed connexin40 and connexin43
    V Valiunas
    Department of Physiology and Biophysics, State University of New York, Stony Brook, New York 11794, USA
    Am J Physiol Heart Circ Physiol 281:H1675-89. 2001
    ..Thus, whereas our biochemical evidence suggests that Cx40 and Cx43 form heteromeric connexons, we conclude that they are functionally insignificant with regard to voltage-dependent gating...
  35. ncbi Mitochondrial connexin 43 impacts on respiratory complex I activity and mitochondrial oxygen consumption
    Kerstin Boengler
    Institut fur Pathophysiologie, Universitatsklinikum Essen, Essen, Germany
    J Cell Mol Med 16:1649-55. 2012
    b>Connexin 43 (Cx43) is present at the sarcolemma and the inner membrane of cardiomyocyte subsarcolemmal mitochondria (SSM)...
  36. ncbi Mutations of the Connexin43 gap-junction gene in patients with heart malformations and defects of laterality
    S H Britz-Cunningham
    Department of Physiology, Loma Linda University, CA 92354, USA
    N Engl J Med 332:1323-9. 1995
    ..We hypothesized that mutations altering sites critical to this regulation would lead to functional or developmental abnormalities of the heart...
  37. ncbi Intramolecular loop/tail interactions are essential for connexin 43-hemichannel activity
    Raf Ponsaerts
    Department Molecular Cell Biology, KU Leuven, Leuven, Belgium
    FASEB J 24:4378-95. 2010
    ..We conclude that intramolecular loop/tail interactions control Cx43-hemichannel activity, laying the basis for developing hemichannel-specific blockers...
  38. pmc Opening of connexin 43 hemichannels is increased by lowering intracellular redox potential
    Mauricio A Retamal
    Núcleo Milenio Inmunología e Inmunoterapia, Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Santiago 4860, Chile
    Proc Natl Acad Sci U S A 104:8322-7. 2007
    ..These findings suggest that lowering intracellular redox potential increases the opening of Cx43 and Cx43-EGFP hemichannels, possibly by action on cytoplasmic cysteine residues in the connexin C terminus...
  39. ncbi Molecular reorganization of Cx43, Zo-1 and Src complexes during the endocytosis of gap junction plaques in response to a non-genomic carcinogen
    Jerome Gilleron
    INSERM U 895, Team 5 Physiopathologic control of germ cell proliferation genomic and non genomic mechanisms, Universite Paris Descartes, 45 rue des Saint Peres, 75006, Paris, France
    J Cell Sci 121:4069-78. 2008
    The gap junction protein connexin 43 (Cx43) exhibits dynamic trafficking that is altered in most tumor cells and in response to carcinogen exposure...
  40. pmc An angiotensin II- and NF-kappaB-dependent mechanism increases connexin 43 in murine arteries targeted by renin-dependent hypertension
    Florian Alonso
    Service of Internal Medicine, Laboratory of Experimental Medicine 19 135S, University Hospital, CHUV 1011 Lausanne, Switzerland
    Cardiovasc Res 87:166-76. 2010
    ..We investigated how connexins of the endothelial cells (ECs; Cx37, Cx40) and smooth muscle cells (SMCs; Cx43, Cx45) of the aorta change during renin-dependent and -independent hypertension...
  41. ncbi Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia
    Wilhelm Roell
    Institute of Physiology I, Life and Brain Center, University of Bonn, Bonn 53105, Germany
    Nature 450:819-24. 2007
    ..VT protection is critically dependent on expression of the gap-junction protein connexin 43 (Cx43; also known as Gja1): SMs genetically engineered to express Cx43 conferred a similar protection to that ..
  42. ncbi Connexin phosphorylation as a regulatory event linked to gap junction internalization and degradation
    Dale W Laird
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochim Biophys Acta 1711:172-82. 2005
    ..This paper will review the evidence that connexin phosphorylation regulates, stimulates or triggers gap junction disassembly, internalization and degradation...
  43. ncbi Connexins act as tumor suppressors in three-dimensional mammary cell organoids by regulating differentiation and angiogenesis
    Elizabeth McLachlan
    Departments of Anatomy and Cell Biology and Physiology and Pharmacology, The University of Western Ontario, London, Ontario, Canada
    Cancer Res 66:9886-94. 2006
    Connexins are tumor suppressors, and human breast connexin 26 (Cx26) and connexin 43 (Cx43) gap junctions are often down-regulated in breast cancer...
  44. pmc Microglia at brain stab wounds express connexin 43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis factor-alpha
    E A Eugenin
    Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago 340, Chile
    Proc Natl Acad Sci U S A 98:4190-5. 2001
    ..This report demonstrates that microglia can communicate with each other through gap junctions that are induced by inflammatory cytokines, a process that may be important in the elaboration of the inflammatory response...
  45. ncbi Efferent vagal nerve stimulation protects heart against ischemia-induced arrhythmias by preserving connexin43 protein
    Motonori Ando
    Department of Cardiovascular Control, Kochi Medical School, Nankoku, Kochi, Japan
    Circulation 112:164-70. 2005
    ..Vagal nerve stimulation has been postulated to contribute to the antifibrillatory effect. Here, we suggest a novel mechanism for the antiarrhythmogenic properties of vagal stimulation during acute MI...
  46. ncbi ATP release from activated neutrophils occurs via connexin 43 and modulates adenosine-dependent endothelial cell function
    Holger K Eltzschig
    Department of Anesthesiology and Intensive Care Medicine, Tubingen University Hospital, Germany
    Circ Res 99:1100-8. 2006
    ..These studies showed that PMN ATP release occurs through connexin 43 (Cx43) hemichannels in a protein/phosphatase-A-dependent manner...
  47. ncbi Gap junctions and connexin expression in human suburothelial interstitial cells
    G P Sui
    Institute of Urology, London, UK
    BJU Int 90:118-29. 2002
    ..To determine whether suburothelial interstitial cells of the human bladder express gap junctions, and if so, to establish their extent and composition, using immunocytochemistry, confocal microscopy and electron microscopy...
  48. ncbi Targeting connexin43 expression accelerates the rate of wound repair
    Cindy Qiu
    Department of Anatomy and Developmental Biology, University College London, Gower St, WC1E 6BT London, UK
    Curr Biol 13:1697-703. 2003
    ..Here we show that direct cell-cell communication through connexin 43 (Cx43) gap junction channels also plays a major role in the wound healing process...
  49. pmc Mechanical strain opens connexin 43 hemichannels in osteocytes: a novel mechanism for the release of prostaglandin
    Priscilla P Cherian
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    Mol Biol Cell 16:3100-6. 2005
    ....
  50. pmc Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium
    James W Smyth
    Cardiovascular Research Institute, Department of Medicine, University of California San Francisco, 505 Parnassus Avenue, San Francisco, CA 94143, USA
    J Clin Invest 120:266-79. 2010
    ..We anticipate that protecting the microtubule-based forward delivery apparatus of connexons could improve cell-cell coupling and reduce ischemia-related cardiac arrhythmias...
  51. pmc Connexin 43 (GJA1) mutations cause the pleiotropic phenotype of oculodentodigital dysplasia
    William A Paznekas
    Department of Pediatrics, Center for Craniofacial Development and Disorders, McKusick Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Am J Hum Genet 72:408-18. 2003
    ..The human connexin 43 gene, or GJA1, is located at human chromosome 6q22-q23 within the candidate region for the oculodentodigital ..
  52. pmc Enhanced transmural fiber rotation and connexin 43 heterogeneity are associated with an increased upper limit of vulnerability in a transgenic rabbit model of human hypertrophic cardiomyopathy
    Crystal M Ripplinger
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Circ Res 101:1049-57. 2007
    ..The transmural fiber angle shift was determined using diffusion tensor MRI. The transmural distribution of connexin 43 was quantified with immunohistochemistry...
  53. pmc Connexin43 deficiency causes delayed ossification, craniofacial abnormalities, and osteoblast dysfunction
    F Lecanda
    Divisions of Bone and Mineral and Infectious Diseases, Department of Internal Medicine, Washington University School of Medicine, Barnes Jewish Hospital, St Louis, Missouri 63110, USA
    J Cell Biol 151:931-44. 2000
    ..Cell to cell signaling, mediated by Cx43 gap junctions, was critical for normal osteogenesis, craniofacial development, and osteoblastic function...
  54. ncbi Basic fibroblast growth factor causes urinary bladder overactivity through gap junction generation in the smooth muscle
    Masaaki Imamura
    Department of Urology, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 606 8507 Japan
    Am J Physiol Renal Physiol 297:F46-54. 2009
    ..Here, we report the involvement of basic fibroblast growth factor (bFGF) and connexin 43, a bladder gap junction protein, in bladder overactivity...
  55. ncbi Ubiquitination and down-regulation of gap junction protein connexin-43 in response to 12-O-tetradecanoylphorbol 13-acetate treatment
    Edward Leithe
    Institute for Cancer Research at The Norwegian Radium Hospital, N 0310 Oslo, Norway
    J Biol Chem 279:50089-96. 2004
    ..Moreover, the results suggest that the proteasome might play an indirect role in Cx43 degradation by affecting the level of monoubiquitin conjugation and trafficking of Cx43 to endosomal compartments...
  56. ncbi Role of the ubiquitin-proteasome pathway in downregulation of the gap-junction protein Connexin43 by TNF-{alpha} in human corneal fibroblasts
    Kazuhiro Kimura
    Departments of Ocular Pathophysiology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
    Invest Ophthalmol Vis Sci 51:1943-7. 2010
    ..The ubiquitin-proteasome system may thus play an important role in the disruption of corneal homeostasis associated with corneal inflammation...
  57. ncbi Gap junction-mediated spread of cell injury and death during myocardial ischemia-reperfusion
    David Garcia-Dorado
    Laboratorio de Cardiologia Experimental, Servicio de Cardiologia, Hospital Universitario Valle de Hebron, Barcelona, Spain
    Cardiovasc Res 61:386-401. 2004
    ..GJ play an important role in the pathophysiology of cell injury and death during myocardial ischemia-reperfusion and are potential targets for new cardioprotective therapeutic strategies...
  58. ncbi Increased susceptibility to urethane-induced lung tumors in mice with decreased expression of connexin43
    José Luis Avanzo
    Laboratory of Experimental Oncology, Department of Pathology, Faculty of Veterinary Medicine and Zootechny, University of Sao Paulo, Avenida Prof Dr Orlando Marques de Paiva 87, CEP 05508 900 São Paulo, Brazil
    Carcinogenesis 25:1973-82. 2004
    ..In conclusion, our results indicate that deletion of one allele of the Cx43 gene clearly favors the carcinogenic effect of urethane administration and results in a higher susceptibility to lung adenoma formation in mice...
  59. ncbi Epidermal growth factor receptor is a common mediator of quinone-induced signaling leading to phosphorylation of connexin-43: role of glutathione and tyrosine phosphatases
    Kotb Abdelmohsen
    Institut fur Biochemie und Molekularbiologie I, Heinrich Heine Universitat Dusseldorf, D 40225 Dusseldorf, Germany
    J Biol Chem 278:38360-7. 2003
    ..In summary, EGFR-dependent signaling was mediated by protein-tyrosine phosphatase inactivation (menadione), GSH depletion (BQ), and redox-cycling (DMNQ), funneling into the same signaling pathway...
  60. pmc Connexon-mediated cell adhesion drives microtissue self-assembly
    Brian Bao
    Department of Molecular Pharmacology, Physiology, and Biotechnology, Center for Biomedical Engineering, Brown University, Providence, RI 02912, USA
    FASEB J 25:255-64. 2011
    ..These findings demonstrate that connexon-mediated cell adhesion and intercellular communication differentially influence microtissue self-assembly, and that their contributions are comparable to those of cadherins...
  61. ncbi Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal
    Steve Catarino
    Centre of Ophthalmology and Vision Sciences, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    Biochem J 437:255-67. 2011
    ..Furthermore, the internalization of Cx43 induced by Cx43-Ub was shown to depend on its interaction with epidermal growth factor receptor substrate 15...
  62. ncbi Downregulation of connexin 43 expression by high glucose induces senescence in glomerular mesangial cells
    XiaoJie Zhang
    Department of Nephrology, Kidney Center and Key Lab of PLA, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, People s Republic of China
    J Am Soc Nephrol 17:1532-42. 2006
    b>Connexin 43 (Cx43) plays an important role in cell differentiation and growth control, but whether it can be regulated by high glucose and whether it can mediate in glomerular mesangial cells (GMC) the phenotype alterations that are ..
  63. pmc Connexin43 interacts with βarrestin: a pre-requisite for osteoblast survival induced by parathyroid hormone
    Nicoletta Bivi
    Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202 5120, USA
    J Cell Biochem 112:2920-30. 2011
    ..These studies demonstrate that βarrestin is a novel Cx43-interacting protein and suggest that, by sequestering βarrestin, Cx43 facilitates cAMP signaling, thereby exerting a permissive role on osteoblast survival induced by PTH...
  64. ncbi Developmental exposure to estrogens alters epithelial cell adhesion and gap junction proteins in the adult rat prostate
    H Habermann
    Department of Urology, University of Illinois, Chicago, Illinois 60612, USA
    Endocrinology 142:359-69. 2001
    ..These findings are significant in light of the data on human prostate cancers where carcinogenesis and progression are associated with loss of E-cadherin and a switch from Cx32 to Cx43 expression in the epithelium...
  65. pmc Misexpression of wingless-related MMTV integration site 5A in mouse mammary gland inhibits the milk ejection response and regulates connexin43 phosphorylation
    Sarah E Baxley
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Biol Reprod 85:907-15. 2011
    ..In summary, we propose that WNT5A inhibits the response to oxytocin and prevents milk ejection through regulation of Cx43 function...
  66. pmc Gating properties of heterotypic gap junction channels formed of connexins 40, 43, and 45
    Mindaugas Rackauskas
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biophys J 92:1952-65. 2007
    ..Furthermore, both fast and slow gating mechanisms of Cx40 exhibit a negative gating polarity...
  67. ncbi Transcription factor Tbx3 is required for the specification of the atrioventricular conduction system
    Martijn L Bakker
    Heart Failure Research Center, Academic Medical Center, Amsterdam, The Netherlands
    Circ Res 102:1340-9. 2008
    ..Our data suggest a mechanism in which Tbx3 represses differentiation into ventricular working myocardium, thereby imposing the conduction system phenotype on cells within its expression domain...
  68. pmc Cell-specific expression of connexins and evidence of restricted gap junctional coupling between glial cells and between neurons
    J E Rash
    Department of Anatomy, Colorado State University, Fort Collins, Colorado 80523, USA
    J Neurosci 21:1983-2000. 2001
    ..Thus, the different cell types of the CNS express different connexins, which define separate pathways for neuronal versus glial gap junctional communication...
  69. pmc Connexin 43 hemichannels mediate the Ca2+ influx induced by extracellular alkalinization
    Kurt A Schalper
    Departamento de Fisiologia, Pontificia Universidad Catolica de Chile, Alameda 340, 4 to Piso, Santiago, 8331150 Chile
    Am J Physiol Cell Physiol 299:C1504-15. 2010
    ..Here, we report that extracellular alkalinization induces opening of connexin 43 hemichannels (Cx43 HCs). Increasing extracellular pH from 7.4 to 8...
  70. ncbi Glial hemichannels and their involvement in aging and neurodegenerative diseases
    Juan A Orellana
    Facultad de Medicina, Departamento de Neurologia, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Rev Neurosci 23:163-77. 2012
    ..Therefore, blockade of hemichannels expressed by glial cells and/or neurons during neuroinflammation might prevent neurodegeneration...
  71. pmc Connexin43 mutation causes heterogeneous gap junction loss and sudden infant death
    David W Van Norstrand
    Mayo Clinic, MN 55905, USA
    Circulation 125:474-81. 2012
    ..GJA1 mutations have been associated with cardiac diseases, including atrial fibrillation. Therefore, GJA1 is a plausible candidate gene for premature sudden death...
  72. pmc Connexin43 modulates cell polarity and directional cell migration by regulating microtubule dynamics
    Richard Francis
    Department of Developmental Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e26379. 2011
    ..Together, these findings suggest the essential role of Cx43 gap junctions in development is mediated by regulation of the tubulin cytoskeleton and cell polarity by Cx43 via a nonchannel function...
  73. ncbi Cell population dynamics (apoptosis, mitosis, and cell-cell communication) during disruption of homeostasis
    M R Wilson
    Department of Pediatrics and Human Development, College of Human Medicine, East Lansing, Michigan 48824, USA
    Exp Cell Res 254:257-68. 2000
    ..This model system is useful for further studies on the relationships among inter- and intracellular mechanisms of homeostasis...
  74. pmc Effects of ageing and streptozotocin-induced diabetes on connexin43 and P2 purinoceptor expression in the rat corpora cavernosa and urinary bladder
    Sylvia O Suadicani
    Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    BJU Int 103:1686-93. 2009
    ....
  75. ncbi Regulation of gap junction intercellular communication by the ubiquitin system
    Ane Kjenseth
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, University of Oslo, Oslo, Norway
    Cell Signal 22:1267-73. 2010
    ..This review discusses the current knowledge about the regulation of intercellular communication via gap junctions by ubiquitination of connexins...
  76. ncbi Neurons control the expression of connexin 30 and connexin 43 in mouse cortical astrocytes
    Annette Koulakoff
    INSERM, U840, College de France, 11 place Marcelin Berthelot, Paris, France
    Glia 56:1299-311. 2008
    ..Altogether these observations indicate that the expression of the two main astrocyte connexins is tightly regulated by neurons...
  77. pmc RhoA GTPase and F-actin dynamically regulate the permeability of Cx43-made channels in rat cardiac myocytes
    Mickael Derangeon
    Institut de Physiologie et Biologie Cellulaires, Universite de Poitiers, F 86022 Poitiers, France
    J Biol Chem 283:30754-65. 2008
    ..The inhibition of Rho downstream kinase effectors had no effect on GJIC. The present results provide further insight into the gating and regulation of junctional channels and identify a new downstream target for the small G-protein RhoA...
  78. pmc Cell autonomous requirement of connexin 43 for osteocyte survival: consequences for endocortical resorption and periosteal bone formation
    Nicoletta Bivi
    Department Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN 46202 5120, USA
    J Bone Miner Res 27:374-89. 2012
    b>Connexin 43 (Cx43) mediates osteocyte communication with other cells and with the extracellular milieu and regulates osteoblastic cell signaling and gene expression...
  79. ncbi Connexin mimetic peptides improve cell migration rates of human epidermal keratinocytes and dermal fibroblasts in vitro
    Catherine S Wright
    Department of Biological and Biomedical Sciences, Glasgow Caledonian University, UK
    Wound Repair Regen 17:240-9. 2009
    ..Furthermore, our results support the therapeutic potential of connexin mimetic peptides to aid wound closure, and provide a simple approach to screening new agents...
  80. ncbi Presence of connexin 43 in subsarcolemmal, but not in interfibrillar cardiomyocyte mitochondria
    Kerstin Boengler
    Institut fur Pathophysiologie, Zentrum fur Innere Medizin, Universitatsklinikum Essen, Essen, Germany
    Basic Res Cardiol 104:141-7. 2009
    ..b>Connexin 43 (Cx43) is located at the inner membrane of SSM, and Cx43 is involved in the cardioprotection by ischemic ..
  81. pmc Compartmentalized connexin 43 s-nitrosylation/denitrosylation regulates heterocellular communication in the vessel wall
    Adam C Straub
    Department of Molecular Physiology and Biological Physics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Arterioscler Thromb Vasc Biol 31:399-407. 2011
    ..To determine whether S-nitrosylation of connexins (Cxs) modulates gap junction communication between endothelium and smooth muscle...
  82. ncbi Atrial tachycardia/fibrillation in the connexin 43 G60S mutant (Oculodentodigital dysplasia) mouse
    Jari M Tuomi
    Department of Physiology and Pharmacology, University of Western Ontario, London, Canada
    Am J Physiol Heart Circ Physiol 300:H1402-11. 2011
    ..In conclusions, while Cx43(G60S/+) mice had severe AT/F, Cx40(-/-) mice were resistant to CCh-induced AT/F...
  83. pmc Staphylococcus aureus-derived peptidoglycan induces Cx43 expression and functional gap junction intercellular communication in microglia
    Sarita Garg
    Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Neurochem 95:475-83. 2005
    ....
  84. ncbi Failure of spermatogenesis in mice lacking connexin43
    W A Roscoe
    Department of Physiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biol Reprod 65:829-38. 2001
    ..These results indicate that intercellular communication via Cx43 channels is required for postnatal expansion of the male germ line...
  85. ncbi Decreased levels of Cx43 gap junctions result in ameloblast dysregulation and enamel hypoplasia in Gja1Jrt/+ mice
    K Toth
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada
    J Cell Physiol 223:601-9. 2010
    ..Together, these data show that reduced levels of Cx43 gap junctions result in ameloblast dysregulation, enamel hypoplasia, and secondary tissue responses...
  86. ncbi The influence of lycopene on the proliferation of human breast cell line (MCF-7)
    F Fornelli
    Institute of Sciences of Food Production, Consiglio Nazionale delle Ricerche, Bari, Italy
    Toxicol In Vitro 21:217-23. 2007
    ..The induction of GJIC by lycopene was confirmed by an increased expression of connexin 43. Collectively, the above data confirm the inhibitor effects of lycopene on MCF-7 cell growth and suggest that ..
  87. ncbi All-trans retinoic Acid regulates cx43 expression, gap junction communication and differentiation in primary lens epithelial cells
    Amy C Long
    The Ohio State University, Interdisciplinary Ph D Program in Nutrition, Department of Human Nutrition, Department of Ophthalmology, Biomedical Engineering Center, College of Optometry, Department of Veterinary Clinical Sciences, Columbus, Ohio, USA
    Curr Eye Res 35:670-9. 2010
    To examine the effect of all-trans retinoic acid (ATRA) treatment on connexin 43 (Cx43) expression, gap junction intercellular communication (GJIC), and cellular differentiation in primary canine lens epithelial cells (LEC).
  88. ncbi A neuroprotective role for gap junctions
    C C Naus
    Department of Anatomy and Cell Biology, The University of Western Ontario, London, Canada
    Cell Commun Adhes 8:325-8. 2001
    ..These findings support the hypothesis that gap junctions play a neuroprotective role against glutamate cytotoxicity...
  89. ncbi Regulation of connexin 43 by basic fibroblast growth factor in the bladder: transcriptional and behavioral implications
    Hiromitsu Negoro
    Kyoto University, Kyoto, Japan
    J Urol 185:2398-404. 2011
    ..factor is a candidate causative factor of detrusor overactivity in bladder outlet obstruction cases through up-regulation of the gap junction protein connexin 43. We addressed the transcriptional and behavioral implications of this axis.
  90. pmc Oscillating fluid flow activation of gap junction hemichannels induces ATP release from MLO-Y4 osteocytes
    Damian C Genetos
    Department of Orthopaedic Surgery, University of California at Davis, Sacramento, California, USA
    J Cell Physiol 212:207-14. 2007
    ..We demonstrate that fluid flow activates hemichannels in MLO-Y4, but not MC3T3-E1, through a mechanism involving protein kinase C, which induces ATP and PGE(2) release...
  91. ncbi Cx43 gap junction gene expression and gap junctional communication in mouse neural crest cells
    C W Lo
    Biology Department, University of Pennsylvania, Philadelphia, USA
    Dev Genet 20:119-32. 1997
    ..known to be important in mediating cell-cell interactions amongst migratory cells, our studies showed that the connexin 43 (Cx43) gap junction gene is widely expressed in mouse neural crest cell lineages...
  92. pmc Human mesenchymal stem cells make cardiac connexins and form functional gap junctions
    Virginijus Valiunas
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, NY 11794 8661, USA
    J Physiol 555:617-26. 2004
    ..hMSC coupling via gap junctions to other cell types provides the basis for considering them as a therapeutic repair or cellular delivery system to syncytia such as the myocardium...
  93. ncbi Alterations in connexin expression in the bladder of patients with urge symptoms
    Jochen Neuhaus
    Department of Urology, Clinic for Urology, University of Leipzig, Leipzig, Germany
    BJU Int 96:670-6. 2005
    ....
  94. pmc Connexin 43 regulates epicardial cell polarity and migration in coronary vascular development
    David Y Rhee
    Laboratory of Developmental Biology, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Development 136:3185-93. 2009
    b>Connexin 43 knockout (Cx43 KO) mice exhibit conotruncal malformations and coronary artery defects...
  95. ncbi Acute downregulation of connexin43 at wound sites leads to a reduced inflammatory response, enhanced keratinocyte proliferation and wound fibroblast migration
    Ryoichi Mori
    Department of Physiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 119:5193-203. 2006
    ....
  96. ncbi Combined reduction of intercellular coupling and membrane excitability differentially affects transverse and longitudinal cardiac conduction
    Mera Stein
    Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, Yalelaan 50, Utrecht, The Netherlands
    Cardiovasc Res 83:52-60. 2009
    ..In this study, we have investigated the effect of isolated and combined reductions in membrane excitability and intercellular coupling on impulse propagation and arrhythmogeneity in genetically modified mice...
  97. ncbi Involvement of the cytoplasmic C-terminal domain of connexin43 in neuronal migration
    Cima Cina
    Department of Cellular and Physiological Sciences, The Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
    J Neurosci 29:2009-21. 2009
    ..This study illustrates that deletion of the C-terminal domain of Cx43 alters neuronal migration in the neocortex...
  98. ncbi Forced alignment of mesenchymal stem cells undergoing cardiomyogenic differentiation affects functional integration with cardiomyocyte cultures
    Daniel A Pijnappels
    Department of Cardiology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Circ Res 103:167-76. 2008
    ..Also, immunocytochemical analysis showed alignment-dependent increases in connexin 43 expression...
  99. ncbi Connexin43 and connexin45 form heteromeric gap junction channels in which individual components determine permeability and regulation
    Agustin D Martinez
    Department of Pediatrics, University of Chicago, Chicago, Ill, USA
    Circ Res 90:1100-7. 2002
    ..Thus, biochemical and electrophysiological evidence suggests that Cx43 and Cx45 extensively mix to form heteromeric channels; however, individual connexin components dominate aspects of the physiological behavior of these channels...
  100. ncbi Upregulation in astrocytic connexin 43 gap junction levels may exacerbate generalized seizures in mesial temporal lobe epilepsy
    Carissa G Fonseca
    Department of Anatomy, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
    Brain Res 929:105-16. 2002
    ..We hypothesize that this surprisingly large upregulation in Cx43 may exacerbate generalized seizures in the progression of MTLE...
  101. ncbi Gap junction remodeling and altered connexin43 expression in the failing human heart
    Sawa Kostin
    Department of Experimental Cardiology, Max Planck Institute, Bad Nauheim, Germany
    Mol Cell Biochem 242:135-44. 2003
    ....

Research Grants69

  1. Structure/Function Analysis of Low pl Connexin Isoforms
    ELLIOT HERTZBERG; Fiscal Year: 2003
    ..Establishing the basis of connexin charge alteration will introduce a new player in chemical modification of proteins and their properties. ..
  2. Microvascular Dysfunction in Hyperhomocysteinemia
    Shawn E Bearden; Fiscal Year: 2012
    ....
  3. Cx43 phosphorylation modulates Kras mediated pancreas cancer progression
    Paul D Lampe; Fiscal Year: 2012
    ..Since drugs that affect Cx43 activity and gap junctional communication are under development, understanding how these factors affect progression could ultimately lead to better treatment of this lethal disease. ..
  4. Mechanisms of Ventricular Tachycardia in Lipotoxic Cardiomyopathy
    JOHN PEARCE MORROW; Fiscal Year: 2013
    ..Further, connexin 43, the main component of the ventricular gap junction, is down regulated at the transcriptional level and the ..
  5. PROTEIN KINASE C IN THE LENS
    DOLORES JEAN TAKEMOTO; Fiscal Year: 2010
    ..previous work from this lab has shown that PKC3 is oxidatively activated and that this activation results in connexin 43 (Cx43), connexin 46 (Cx46), and connexin 50 (Cx50) gap junction inhibition...
  6. Connexin 43 in Bone Remodeling
    Roberto Civitelli; Fiscal Year: 2013
    ..Results will establish the mechanisms by which Cx43 modulates cortical bone and how such mechanisms cause the skeletal abnormalities of ODDD, findings that may be translated to clinical settings and lead to pharmacologic targeting. ..
  7. GENETIC STUDIES OF ODDD
    Ethylin Wang Jabs; Fiscal Year: 2010
    ..identified mutations (missense and small duplication) in the gap junction protein GJA1, also referred to as connexin 43, in ODDD patients...
  8. Implications of Endothelial-Myocyte Uncoupling in Cardiac Arrhythmia
    Suresh C Tyagi; Fiscal Year: 2010
    ..These studies will delineate the mechanism of Hcy-dependent endothelial-myocyte uncoupling in cardiac arrhythmia and failure, and will have therapeutic ramifications for sudden cardiac death. ..
  9. Satellite Glial Cells
    Luc Jasmin; Fiscal Year: 2013
    ..an enzyme (guanylyl cyclase), a calcium-activated potassium channel (SK3), and a component of the gap junction (connexin 43)...
  10. Renin angiotensin system and connexin43
    Samuel C Dudley; Fiscal Year: 2013
    ..Specifically, this application will give a more complete picture of the pro-arrhythmic effects of AngII on heart, which could lead to new diagnostic and therapeutic interventions. ..
  11. Mechanisms of Intracellular NAMPT-regulated GSNOR in Vessel Wall
    ADAM CARL STRAUB; Fiscal Year: 2012
    ..nitric oxide group from a cysteine-thiol side chain, serves as an important post-translational modification on connexin 43 gap junction (GJ) proteins, and that this modification is associated with control of resistance arterial tone...
  12. Mechanisms of Intracellular NAMPT-regulated GSNOR in Vessel Wall
    ADAM CARL STRAUB; Fiscal Year: 2013
    ..nitric oxide group from a cysteine-thiol side chain, serves as an important post-translational modification on connexin 43 gap junction (GJ) proteins, and that this modification is associated with control of resistance arterial tone...
  13. SMOOTH MUSCLE CELL PROLIFERATION IN HUMAN CORONARY ARTERY BYPASS GRAFTS
    Devendra K Agrawal; Fiscal Year: 2012
    ..The information obtained from this study should provide an opportunity to formulate superior therapeutic approaches. ..
  14. Connexin 43 Hemichannels and Signaling In Bone
    LILIAN IRENE PLOTKIN; Fiscal Year: 2012
    ..We expect that this work will provide opportunities to improve the treatment of diseases with increased bone fragility. ..
  15. Cyclic nucleotide permeability of connexin mutants related to genetic disease
    Virginijus Valiunas; Fiscal Year: 2013
    ..The results of the proposed research will establish a baseline for understanding the role of perm-selectivity as a potential determinant of disease states such as oculodentodigital dysplasia and atrial fibrillation. ..
  16. Mechanisms of Mitochondrial Dysfunction in Brain Vasculature
    Neetu Tyagi; Fiscal Year: 2013
    ..These studies will demonstrate the novel mechanism of cerebrovascular remodeling and have therapeutic ramifications for mitochondrial repair in cerebral ischemic stroke. ..
  17. Connexin Distribution in Physiological Versus Pathological Cardiac Hypertrophy
    Michael R Zile; Fiscal Year: 2013
    ..pathological hypertrophy, with ex- tensively characterized cytoskeletal properties in each setting. ..
  18. MOLECULAR MECHANISMS OF THE MYOTONIC DYSTROPHY MUTATION
    MANI SUBRAMANIAM MAHADEVAN; Fiscal Year: 2013
    ..5, and profound disturbances in connexin 40 (Cx40) and connexin 43 (Cx43), proteins that are targets of Nkx2.5 and crucial for normal cardiac conduction...
  19. Mechanisms for Stem Cell Differentiation into Cardiac Myocytes
    Margaret Loewy Kirby; Fiscal Year: 2010
    ..the following sequential events drive naive stem cell differentiation into a cardiomyocyte: shared functional connexin 43 (Cx43)-derived gap junctions develop between the stem cell and an adjacent contracting cardiomyocyte (Cx43 is ..
  20. Development of a novel connexin-based peptide for the treatment of corneal injury
    GAUTAM SUDHIR GHATNEKAR; Fiscal Year: 2012
    ..FirstString's lead novel bioengineered peptide dubbed ACT1, is based on the C-terminal sequence of connexin 43 (Cx43), and shows great promise in modulating intercellular communication...
  21. Mechanism(s) of CD8 T cell-mediated Chlamydia-induced reproductive pathology
    ASHLESH KRISHNA MURTHY; Fiscal Year: 2013
    ..b>Connexin 43, also expressed on oviduct epithelium, has been shown to be involved in the transfer of antigenic peptides from ..
  22. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul D Lampe; Fiscal Year: 2013
    ....
  23. STRUCTURAL ANALYSIS OF GAP JUNCTION TRAFFICKING
    Gina E Sosinsky; Fiscal Year: 2013
    ..We investigate the connexin43 trafficking process using an imaging based approach examining the hierarchy of connexin43 phosphorylation events and where within the cell cycle, connexin43-kinase(s) interactions occurs. ..
  24. JNK Suppression of Connexin43 Enhances Atrial Fibrillation in Aged Atria
    Xun Ai; Fiscal Year: 2013
    ..Our long-term goal, understanding the molecular mechanism of AF development in aged hearts with co-existing cardiovascular diseases such as HF, will be greatly advanced by the results of the current proposal. ..
  25. SIGNAL TRANSDUCTION AT FERTILIZATION
    Laurinda A Jaffe; Fiscal Year: 2012
    ..These studies will be complemented by investigations of the phosphorylation of connexin 43 on specific regulatory sites...
  26. The effect of phthalates on the heart: molecular pathways and clinical relevance
    Nikki Gillum Posnack; Fiscal Year: 2013
    ..In contrast, little is known about the adverse effects of DEHP on the heart, and awareness of its potential cardiotoxicity is essentially non-existent. ..
  27. Determining the role of sema3d during fin regeneration
    MARY KATHRYN IOVINE; Fiscal Year: 2013
    ....
  28. Connexin 43 gap junction dynamics in the diabetic heart
    Joseph A Palatinus; Fiscal Year: 2013
    ..Zonula occludens-1 (ZO-1) binds Connexin 43 (Cx43), and this interaction is important for gapjunction remodeling...
  29. Intracellular Sorting of Connexin 43 by the Ubiquitin Ligase Wwp1
    LYDIA ELEANOR MATESIC; Fiscal Year: 2013
    ..displays myocyte hypertrophy and disarray, disorganization of intercalated discs with altered localization of Connexin 43 (Cx43), and, usually, increased amounts of interstitial fibrosis...
  30. Caveolae, mitochondria, and cardiac protection.
    Hemal H Patel; Fiscal Year: 2012
    ..Certain proteins found in the heart control the degree of injury. It is the goal of this study to discover key proteins involved in protection against a heart attack injury so that drugs can be appropriately designed. ..
  31. Electro-mechanical Resynchronization Therapy For Arrhythmogenic Right Ventricular
    Harikrishna Tandri; Fiscal Year: 2012
    ..Finally, insights gained from gap junctional protein expression in ARVC may stimulate research in the role of gap junctional proteins in arrhythmogenesis in other inherited cardiomyopathies. (End of Abstract) ..
  32. A gap junction-independent role for Connexin43 in cardiac electrophysiology
    Mario Delmar; Fiscal Year: 2013
    ..Overall, our experiments challenge the prevailing concept that, in a structurally normal heart, Cx43-dependent arrhythmias are only consequent to the loss of gap junction channels between cells. ..
  33. Altered phosphorylation of connexin 43 contributes to increased gap-junction coup
    HEIDI GRABENSTATTER; Fiscal Year: 2013
    ..astrocytes3 suggests that increased open probability of gap- junction hemi channels via dephosphorylating of connexin 43 in hippocampal astrocytes may contribute to the progression of epileptogenesis...
  34. Mechanisms of Gap Junction Regulation
    Paul L Sorgen; Fiscal Year: 2013
    ....
  35. Mechanisms enhancing functional coupling between native and embryonic stem cell d
    Karen Maass; Fiscal Year: 2013
    ....
  36. GAP JUNCTIONS AND IONIC CURRENTS IN DEVELOPING HEART
    Jenny J Yang; Fiscal Year: 2013
    ..These combined experimental approaches will identify new molecular targets on the connexin proteins for the possible pharmacological modulation of cardiac electrical communication by new therapeutic agents. ..
  37. Connexin-Mediated Hemichannel Activity in Experimental Periodontitis
    Karen F Ross; Fiscal Year: 2012
    ..to fluid movement above the cells (mechanical stimulation), and oral keratinocytes express predominately connexin 43 (Cx43). We hypothesize, therefore, that P...
  38. Restoration of Skin Structure and Function Post-Wounding
    GAUTAM SUDHIR GHATNEKAR; Fiscal Year: 2010
    ..This technology provides promise for improving healing, reduced scarring and restoration of skin structure and function following injuries for millions of people. ..
  39. Mechanobiology of Rotator Cuff Development
    Stavros Thomopoulos; Fiscal Year: 2013
    ..We will also study the recovery potential of the developing tendon enthesis subjected to varying durations of paralysis and recovery. ..
  40. Cell Biology of Astrocytes in the Optic Nerve Head
    TATJANA CLAUDIA JAKOBS; Fiscal Year: 2013
    ..Our goal is to study the reaction of individual astrocytes to injury and glaucomatous degeneration in more detail than has been possible before. ..
  41. INTERCELLULAR COMMUNICATION IN THE LENS
    Lisa Ebihara; Fiscal Year: 2013
    ..Three different connexin proteins (Connexin 43 (Cx43), Connexin 46 (Cx46), Connexin 50 (Cx50)) have been identified in lens fibers...
  42. Homeostatic Roles of Connexin43 in Response to DNA Damage
    DANNY SAM ROH; Fiscal Year: 2013
    ..This proposal studies a potential coping mechanism to DNA damage that involves changes in how damaged cells communicate with one another to maintain their function and viability. ..
  43. Regulation of Vascular Stabilization by Connexin 43
    Linda J Metheny-Barlow; Fiscal Year: 2013
    ..Our preliminary data demonstrate that the junctional molecule Connexin 43 (Cx43) plays a previously unidentified role in stabilization of normal blood vessels...
  44. Cellular Cardiomyoplasty for Chronic Heart Failure
    Joshua M Hare; Fiscal Year: 2013
    ..resulting from a combination of autologous CSCs and MSCs, 2) assess the impact of cell-cell communication by connexin 43 (Cx43)-mediated gap junctions on cardiac regeneration, and 3) assess the cell- cell interaction and efficacy of ..
  45. SiRNA therapeutics: Gap junction delivery in vitro and in vivo
    Richard T Mathias; Fiscal Year: 2013
    ..By a combination of experiment and mathematical modeling we seek to determine the ability of cellular delivery of siRNA to penetrate tissues in vitro and in vivo. ..
  46. Mechanobiology of 3D Trabecular Bone Explants
    X Edward Guo; Fiscal Year: 2013
    ..of 3D bovine trabecular bone explants with seeded primary bovine OBs depend on the gap junctions/hemi-channels connexin 43 (Cx43) in OCYs...
  47. Regulation of Osteoblast Differentiation and Function by Connexin 43
    Joseph P Stains; Fiscal Year: 2013
    ..The gap junction protein connexin 43, which permits the direct cell-to-cell communication of signals between osteoblasts and osteocytes, has been ..
  48. Hemichannels, astrocytic release, and neuropathic pain
    Maiken Nedergaard; Fiscal Year: 2013
    ..The proposed studies will provide a step-by-step analysis of neuron- glia interactions initiated by nerve injury and may comprise an efficient means to prevent and treat chronic pain. ..
  49. Gap Junctional Patterning in Arrhythmic Heart
    Robert G Gourdie; Fiscal Year: 2013
    ..This project will deliver new insights on the function of a protein essential to a stable heartbeat called connexin43. The project aims to provide novel approaches to the prevention and treatment of heart attacks. ..
  50. Modulation of regulatory cell function in cancer immunotherapy
    PIOTR J KRAJ; Fiscal Year: 2013
    ..This will be accomplished by modulating the expression of connexin 43, a molecule identified to control suppressor function of TR cells and expression of Foxp3...
  51. Tissue Engineered Repair of Cranial Facial Muscle
    EMILY LYNN ONGSTAD; Fiscal Year: 2013
    ..It is our aim to generate a replacement for facial muscle defects that will lead to regrowth of normal, functional tissue with reduced scarring. ..
  52. Malignant melanoma: Regulation by AP-2 and PAR-1
    MENASHE BARELI; Fiscal Year: 2012
    ..These genes include MCAM/MUC18, Connexin 43, Maspin, RUNX3, IL-8, Ang-2, GRO, ACRP30, and GITR-ligand...
  53. Efficacy of Novel Wnt Modulator in Colon Cancer
    Heinz Josef Lenz; Fiscal Year: 2013
    ..the expression of Wnt/[unreadable]-catenin regulated genes including survivin, EGFR, VEGF, EphB2, S100A4,CD44, connexin 43 and cyclin D1, which play important roles in initiation, proliferation, angiogenesis, metastasis and drug ..
  54. Regulation of Cx26 and Cx32 Channels by Cytosolic Interdomain Interactions
    Andrew L Harris; Fiscal Year: 2013
    ....
  55. Astrocyte connexin43 containing channels amplify CNS dysfunction in NeuroAIDS
    Eliseo A Eugenin; Fiscal Year: 2013
    ..The results obtained from this proposal should indicate potential novel therapeutic targets to limit the devastating consequences of NeuroAIDS. PHS 398/2590 (Rev. 06/09) Page Continuation Format Page ..
  56. Role of Desmosomes in Cardiac Electrical Function
    Mario Delmar; Fiscal Year: 2013
    ..abstract_text> ..
  57. Ion Channel Function in Arrhythmogenic Cardiac Cell
    PENELOPE ALTMAN BOYDEN; Fiscal Year: 2010
    ....
  58. Connexin43 Regulation and Cardiovascular Function
    Glenn I Fishman; Fiscal Year: 2013
    ....
  59. Bisphosphonate binding to connexin43-expressing cells
    Teresita Bellido; Fiscal Year: 2007
    ....
  60. Damage signaling from irradiated to non-irradiated cells
    EDOUARD AZZAM; Fiscal Year: 2004
    ..They are also relevant to diagnostic and therapeutic nuclear medicine where radionuclides are being investigated to detect various diseases and treat cancer. ..
  61. MOLECULAR MECHANISMS CELL COMMUNICATIONS/ATHEROGENESIS
    Peter Davies; Fiscal Year: 2002
    ..that both vascular endothelial and smooth muscle cells in tissue culture express mRNA for the Gj protein connexin 43 (cxn43)...
  62. MOLECULAR MECHANISMS OF CELL COMMUNICATION IN ATHEROGENE
    Peter Davies; Fiscal Year: 1993
    ..that both vascular endothelial and smooth muscle cells in tissue culture express mRNA for the Gj protein connexin 43 (cxn43)...
  63. Local Signals and Macromolecular Architecture in Heart
    Terry Rogers; Fiscal Year: 2006
    ..abstract_text> ..
  64. TURNING SCAR INTO VIABLE MYOCARDIUM
    Race Kao; Fiscal Year: 2004
    ..Proteins specific to cardiac or skeletal muscel (TnI, myosin, connexin 43, myogenin) will be used to indicate the phenotypes of regenerated muscle by identifying the specific mRNA (..
  65. Homogeneous Transfer of Connexin 43 to Prevent Atrial Fibrillation in Pigs
    Hao Qin; Fiscal Year: 2007
    ..We will test the hypothesis that overexpression of connexin 43 (Cx43) will increase conduction velocity through the atria in the absence of significant changes in tissue ..
  66. Communication Between Laminin 5 and Gap Junctions
    William Carter; Fiscal Year: 2005
    ..b>Connexin 43 (Cx43) is the predominant constituent of GJs in the epidermis...
  67. PINEAL GAP JUNCTION--CHARACTERIZATION AND REGULATION
    JUAN SAEZ; Fiscal Year: 1992
    ..Connexin 26 is restricted to pinealocytes and connexin 43 is found only in the astrocytes]...
  68. The Role of Gap Junctions in Peripheral Odor Coding
    Chunbo Zhang; Fiscal Year: 2004
    ..we have shown that mature olfactory receptor neurons (ORNs) express gap junction protein subunits connexin 36, connexin 43, and connexin 45...
  69. REGULATORY MECHANISMS OF CARDIAC REPOLARIZATION
    Michael Tamkun; Fiscal Year: 2005
    ..Given the role that both Kv channels and connexins play in cardiac and vascular smooth muscle, the proposed research will significantly advance our understanding of electrical excitability in the cardiovascular system. ..