membrane proteins

Summary

Summary: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Top Publications

  1. ncbi Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes
    A Krogh
    Center for Biological Sequence Analysis, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
    J Mol Biol 305:567-80. 2001
  2. ncbi Induction of autophagy and inhibition of tumorigenesis by beclin 1
    X H Liang
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Nature 402:672-6. 1999
  3. pmc Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death
    M C Wei
    Howard Hughes Medical Institute, Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 292:727-30. 2001
  4. pmc The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus
    Abraham L Brass
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard Medical School, Charlestown, MA 02129, USA
    Cell 139:1243-54. 2009
  5. doi The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1
    Philip A Gregory
    Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia
    Nat Cell Biol 10:593-601. 2008
  6. doi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
  7. pmc The Beclin 1 network regulates autophagy and apoptosis
    R Kang
    Department of Surgery, Hillman Cancer Center, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Cell Death Differ 18:571-80. 2011
  8. pmc Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor
    Zhenyu Yue
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:15077-82. 2003
  9. pmc XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response
    Ann Hwee Lee
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Mol Cell Biol 23:7448-59. 2003
  10. ncbi IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA
    Marcella Calfon
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Nature 415:92-6. 2002

Detail Information

Publications311 found, 100 shown here

  1. ncbi Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes
    A Krogh
    Center for Biological Sequence Analysis, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
    J Mol Biol 305:567-80. 2001
    ..Additionally, TMHMM can discriminate between soluble and membrane proteins with both specificity and sensitivity better than 99 %, although the accuracy drops when signal peptides are ..
  2. ncbi Induction of autophagy and inhibition of tumorigenesis by beclin 1
    X H Liang
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Nature 402:672-6. 1999
    ..These findings suggest that decreased expression of autophagy proteins may contribute to the development or progression of breast and other human malignancies...
  3. pmc Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death
    M C Wei
    Howard Hughes Medical Institute, Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 292:727-30. 2001
    ..Thus, activation of a "multidomain" proapoptotic member, BAX or BAK, appears to be an essential gateway to mitochondrial dysfunction required for cell death in response to diverse stimuli...
  4. pmc The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus
    Abraham L Brass
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard Medical School, Charlestown, MA 02129, USA
    Cell 139:1243-54. 2009
    ..Collectively this work identifies a family of antiviral restriction factors that mediate cellular innate immunity to at least three major human pathogens...
  5. doi The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1
    Philip A Gregory
    Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia
    Nat Cell Biol 10:593-601. 2008
    ..Expression of the miR-200 family was also lost in regions of metaplastic breast cancer specimens lacking E-cadherin. These data suggest that downregulation of the microRNAs may be an important step in tumour progression...
  6. doi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
    ..Here we review the evidence for how this principle combines the potential for sphingolipid-cholesterol self-assembly with protein specificity to selectively focus membrane bioactivity...
  7. pmc The Beclin 1 network regulates autophagy and apoptosis
    R Kang
    Department of Surgery, Hillman Cancer Center, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Cell Death Differ 18:571-80. 2011
    ..Here, we summarize new findings regarding the organization and function of the Beclin 1 network in cellular homeostasis, focusing on the cross-regulation between apoptosis and autophagy...
  8. pmc Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor
    Zhenyu Yue
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:15077-82. 2003
    ..They both provide a biological explanation for recent evidence implicating beclin 1 in human cancer and suggest that mutations in other genes operating in this pathway may contribute to tumor formation through deregulation of autophagy...
  9. pmc XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response
    Ann Hwee Lee
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Mol Cell Biol 23:7448-59. 2003
    ..Further, our observations suggest the existence of additional, as-yet-unknown, key regulators of the UPR...
  10. ncbi IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA
    Marcella Calfon
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Nature 415:92-6. 2002
    ..Our findings suggest that physiological ER load regulates a developmental decision in higher eukaryotes...
  11. pmc WoLF PSORT: protein localization predictor
    Paul Horton
    Computational Biology Research Center, AIST, Tokyo, Japan
    Nucleic Acids Res 35:W585-7. 2007
    ..Taken together, this information allows a user to understand the evidence (or lack thereof) behind the predictions made for particular proteins. WoLF PSORT is available at wolfpsort.org...
  12. pmc Neuroligins and neurexins link synaptic function to cognitive disease
    Thomas C Sudhof
    Neuroscience Institute, Department of Molecular and Cellular Physiology, Stanford University, 1050 Arastradero Road B249, Palo Alto, California 94304, USA
    Nature 455:903-11. 2008
    ..In humans, alterations in genes encoding neurexins or neuroligins have recently been implicated in autism and other cognitive diseases, linking synaptic cell adhesion to cognition and its disorders...
  13. ncbi A human homologue of the Drosophila Toll protein signals activation of adaptive immunity
    R Medzhitov
    Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520 8011, USA
    Nature 388:394-7. 1997
    ..1, which is required for the activation of naive T cells...
  14. ncbi Osteoblastic cells regulate the haematopoietic stem cell niche
    L M Calvi
    Endocrine Unit, Department of Medicine, Center for Human Genetics and Molecular Pediatric Disease, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Nature 425:841-6. 2003
    ..Niche constituent cells or signalling pathways provide pharmacological targets with therapeutic potential for stem-cell-based therapies...
  15. pmc Organizing the cell cortex: the role of ERM proteins
    Richard G Fehon
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Nat Rev Mol Cell Biol 11:276-87. 2010
    ..Recent studies examining the structure and in vivo functions of ERMs have greatly advanced our understanding of the importance of membrane-cytoskeleton interactions...
  16. pmc Mitochondria supply membranes for autophagosome biogenesis during starvation
    Dale W Hailey
    The Eunice Kennedy Shriver National Institutes of Child Health and Human Development, 18 Library Drive, Bethesda, MD 20892, USA
    Cell 141:656-67. 2010
    ..Disruption of mitochondria/ER connections by mitofusin2 depletion dramatically impairs starvation-induced autophagy. Mitochondria thus play a central role in starvation-induced autophagy, contributing membrane to autophagosomes...
  17. pmc STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx
    Jen Liou
    Department of Molecular Pharmacology, Stanford University Medical School, California 94305, USA
    Curr Biol 15:1235-41. 2005
    ..Our study suggests that STIM proteins function as Ca(2+) store sensors in the signaling pathway connecting Ca(2+) store depletion to Ca(2+) influx...
  18. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
    ..This complex array of signals and recognition proteins ensures the dynamic but accurate distribution of transmembrane proteins to different compartments of the endosomal-lysosomal system...
  19. pmc Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web server
    LUKAS KALL
    Center for Genomics and Bioinformatics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Nucleic Acids Res 35:W429-32. 2007
    ..We here present a web interface (http://phobius.cgb.ki.se and http://phobius.binf.ku.dk) to access Phobius...
  20. ncbi Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy
    Sophie Pattingre
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Cell 122:927-39. 2005
    ..This antiautophagy function of Bcl-2 may help maintain autophagy at levels that are compatible with cell survival, rather than cell death...
  21. ncbi Binding of hepatitis C virus to CD81
    P Pileri
    IRIS, Chiron, Siena 53100, Italy
    Science 282:938-41. 1998
    ..Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro...
  22. pmc JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy
    Yongjie Wei
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 30:678-88. 2008
    ..These findings define a mechanism that cells use to regulate autophagic activity in response to nutrient status...
  23. ncbi A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function
    Stefan Feske
    The CBR Institute for Biomedical Research, and the Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 441:179-85. 2006
    ..We propose that Orai1 is an essential component or regulator of the CRAC channel complex...
  24. ncbi Genetic variants regulating ORMDL3 expression contribute to the risk of childhood asthma
    Miriam F Moffatt
    National Heart and Lung Institute, Imperial College, London SW3 6LY, UK
    Nature 448:470-3. 2007
    ..The results indicate that genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma...
  25. ncbi ER stress induces cleavage of membrane-bound ATF6 by the same proteases that process SREBPs
    J Ye
    Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 6:1355-64. 2000
    ..ATF6 processing did not require SCAP, which is essential for SREBP processing. We conclude that S1P and S2P are required for the ER stress response as well as for lipid synthesis...
  26. ncbi Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response
    A Bertolotti
    Skirball Institute of Biomolecular Medicine, Departments of Medicine and Cell Biology and the Kaplan Cancer Center, New York University School of Medicine, New York, New York 10016, USA
    Nat Cell Biol 2:326-32. 2000
    ..These findings are consistent with a model in which BiP represses signalling through PERK and IRE1 and protein misfolding relieves this repression by effecting the release of BiP from the PERK and IRE1 lumenal domains...
  27. doi The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
    Camilla Raiborg
    Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Nature 458:445-52. 2009
    Selective trafficking of membrane proteins to lysosomes for destruction is required for proper cell signalling and metabolism...
  28. ncbi Multifunctional strands in tight junctions
    S Tsukita
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Nat Rev Mol Cell Biol 2:285-93. 2001
    ..New insights into the molecular architecture of tight junctions allow us to now discuss the structure and functions of this unique cell-cell adhesion apparatus in molecular terms...
  29. pmc Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia
    Huafeng Zhang
    Vascular Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:10892-903. 2008
    ....
  30. pmc STIM1, an essential and conserved component of store-operated Ca2+ channel function
    Jack Roos
    Torrey Pines Therapeutics, Inc, La Jolla, CA 92037, USA
    J Cell Biol 169:435-45. 2005
    ..We propose that STIM1, a ubiquitously expressed protein that is conserved from Drosophila to mammalian cells, plays an essential role in SOC influx and may be a common component of SOC and CRAC channels...
  31. pmc Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusin
    Elena Ziviani
    Department of Biomedical Sciences, Medical Research Council Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 107:5018-23. 2010
    ..We hypothesize that Mfn ubiquitination may provide a mechanism by which terminally damaged mitochondria are labeled and sequestered for degradation by autophagy...
  32. pmc Mutations of the X-linked genes encoding neuroligins NLGN3 and NLGN4 are associated with autism
    Stephane Jamain
    Laboratoire de génétique humaine et fonctions cognitives, Universite Paris 7, Institut National de la Santé et la Recherche Médicale E0021, 75015 Paris, France
    Nat Genet 34:27-9. 2003
    ..These mutations affect cell-adhesion molecules localized at the synapse and suggest that a defect of synaptogenesis may predispose to autism...
  33. ncbi A hidden Markov model for predicting transmembrane helices in protein sequences
    E L Sonnhammer
    National Center for Biotechnology Information, NLM NIH, Bethesda, Maryland 20894, USA
    Proc Int Conf Intell Syst Mol Biol 6:175-82. 1998
    ..The same accuracy was achieved on a larger dataset of 160 proteins. These results compare favourably with existing methods...
  34. pmc Atg9 vesicles are an important membrane source during early steps of autophagosome formation
    Hayashi Yamamoto
    Frontier Research Center, Tokyo Institute of Technology, Yokohama 226 8503, Japan
    J Cell Biol 198:219-33. 2012
    ..Our findings provide conclusive linkage between the cytoplasmic Atg9 vesicles and autophagosomal membranes and offer new insight into the requirement for Atg9 vesicles at the early step of autophagosome formation...
  35. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
    ..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
  36. ncbi Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry
    Matthew J Evans
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Ave, New York 10021, USA
    Nature 446:801-5. 2007
    ..With CLDN1 we have identified a novel key factor for HCV entry and a new target for antiviral drug development...
  37. ncbi Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease
    R Sherrington
    Department of Medicine Neurology, University of Toronto, Ontario, Canada
    Nature 375:754-60. 1995
    ..Because these changes occurred in conserved domains of this gene, and are not present in normal controls, they are likely to be causative of AD3...
  38. ncbi Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells
    Johan H van Es
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    Nature 435:959-63. 2005
    ..Our data indicate that gamma-secretase inhibitors, developed for Alzheimer's disease, might be of therapeutic benefit in colorectal neoplastic disease...
  39. pmc Genetic variation in PNPLA3 confers susceptibility to nonalcoholic fatty liver disease
    Stefano Romeo
    Donald W Reynolds Cardiovascular Clinical Research Center, Eugene McDermott Center for Human Growth and Development, Dallas, TX 75390, USA
    Nat Genet 40:1461-5. 2008
    ..Thus, variation in PNPLA3 contributes to ancestry-related and inter-individual differences in hepatic fat content and susceptibility to NAFLD...
  40. pmc Functional and physical interaction between Bcl-X(L) and a BH3-like domain in Beclin-1
    M Chiara Maiuri
    INSERM U848, Villejuif, France
    EMBO J 26:2527-39. 2007
    ..BH3-only proteins and pharmacological BH3 mimetics induce autophagy by competitively disrupting the interaction between Beclin-1 and Bcl-2 or Bcl-X(L)...
  41. ncbi Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members
    Michael Certo
    Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Cancer Cell 9:351-65. 2006
    ..Our data allow us to distinguish a cellular state we call "primed for death," which can be determined by BH3 profiling and which correlates with dependence on antiapoptotic family members for survival...
  42. pmc IFITM3 inhibits influenza A virus infection by preventing cytosolic entry
    Eric M Feeley
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Charlestown, Massachusetts, United States of America
    PLoS Pathog 7:e1002337. 2011
    ..Therefore these findings are likely to have significance for the intrinsic immune system's neutralization of a diverse array of threats...
  43. pmc Tim-3 expression defines a novel population of dysfunctional T cells with highly elevated frequencies in progressive HIV-1 infection
    R Brad Jones
    Department of Immunology, University of Toronto, Toronto, ON, Canada
    J Exp Med 205:2763-79. 2008
    ..Blocking the Tim-3 signaling pathway restored proliferation and enhanced cytokine production in HIV-1-specific T cells. Thus, Tim-3 represents a novel target for the therapeutic reversal of HIV-1-associated T cell dysfunction...
  44. ncbi Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls
    R J Kaufman
    Department of Biological Chemistry and Howard Hughes Medical Institute, The University of Michigan Medical Center, Ann Arbor, Michigan 48109 0650 USA
    Genes Dev 13:1211-33. 1999
  45. ncbi DRAM, a p53-induced modulator of autophagy, is critical for apoptosis
    Diane Crighton
    Tumour Cell Death Laboratory, Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
    Cell 126:121-34. 2006
    ..Collectively therefore, these studies not only report a stress-induced regulator of autophagy but also highlight the relationship of DRAM and autophagy to p53 function and damage-induced programmed cell death...
  46. pmc Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells
    N Mizushima
    Unit Process and Combined Circuit, PRESTO, Japan Science and Technology Corporation, Kawaguchi 332 0012, Japan
    J Cell Biol 152:657-68. 2001
    ..We also show that Apg12-Apg5 is required for targeting of a mammalian Aut7/Apg8 homologue, LC3, to the isolation membranes. These results suggest that the Apg12-Apg5 conjugate plays essential roles in isolation membrane development...
  47. pmc Upregulation of Tim-3 and PD-1 expression is associated with tumor antigen-specific CD8+ T cell dysfunction in melanoma patients
    Julien Fourcade
    Department of Medicine and Division of Hematology Oncology, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15213, USA
    J Exp Med 207:2175-86. 2010
    ..Collectively, our findings support the use of Tim-3-Tim-3L blockade together with PD-1-PD-L1 blockade to reverse tumor-induced T cell exhaustion/dysfunction in patients with advanced melanoma...
  48. pmc Three-dimensional structures of membrane proteins from genomic sequencing
    Thomas A Hopf
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 149:1607-21. 2012
    ....
  49. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
    ..Key findings from our studies in cell culture were recapitulated in photoreceptors expressing mutant rhodopsin in animal models of retinitis pigmentosa...
  50. ncbi Protein sensors for membrane sterols
    Joseph L Goldstein
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell 124:35-46. 2006
    ..Here, we discuss recent advances that explain how cells employ an ensemble of membrane-embedded proteins to monitor sterol concentrations and adjust sterol synthesis and uptake...
  51. pmc Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 19:5360-72. 2008
    ..These results suggest that mammalian cells have at least two distinct class III PI3-kinase complexes, which may function in different membrane trafficking pathways...
  52. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
    ..These results indicate that AIF is a mitochondrial effector of apoptotic cell death...
  53. doi The tail of integrins, talin, and kindlins
    Markus Moser
    Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Science 324:895-9. 2009
    ..This review focuses on the mechanisms whereby two key proteins, talin and the kindlins, regulate integrin activation by binding the tails of integrin-beta subunits...
  54. ncbi BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly
    Tomomi Kuwana
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Mol Cell 17:525-35. 2005
    ..In this model, the simple inhibition of antiapoptotic functions is insufficient to induce apoptosis unless a direct activator of Bax or Bak is present...
  55. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
    ..membrane (ONM) is continuous with the endoplasmic reticulum, it is nevertheless enriched in several integral membrane proteins, including nesprin 2 Giant (nesp2G), an 800-kD protein featuring an NH(2)-terminal actin-binding domain...
  56. pmc The mitochondrial fusion-promoting factor mitofusin is a substrate of the PINK1/parkin pathway
    Angela C Poole
    Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 5:e10054. 2010
    ....
  57. ncbi Blue native PAGE
    Ilka Wittig
    Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt, Germany
    Nat Protoc 1:418-28. 2006
    ..These protocols for 2D and 3D PAGE can be completed in 2 and 3 days...
  58. pmc STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling
    Hiroki Ishikawa
    Department of Medicine and Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, Miami, Florida 33136, USA
    Nature 455:674-8. 2008
    ..Thus, as well as identifying a regulator of innate immune signalling, our results imply a potential role for the translocon in innate signalling pathways activated by select viruses as well as intracellular DNA...
  59. pmc Sequence variants at the TERT-CLPTM1L locus associate with many cancer types
    Thorunn Rafnar
    deCODE Genetics, Sturlugata 8, 101 Reykjavik, Iceland
    Nat Genet 41:221-7. 2009
    ..However, neither variant could fully account for the association of the other. rs2736098 corresponds to A305A in the telomerase reverse transcriptase (TERT) protein and rs401681 is in an intron of the CLPTM1L gene...
  60. pmc The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus
    Elisa Scarselli
    Istituto di Ricerche di Biologia Molecolare P Angeletti IRBM, Via Pontina Km 30 600, 00040 Pomezia Rome, Italy
    EMBO J 21:5017-25. 2002
    ..Finally, E2 recognition by SR-BI was competed out in an isolate-specific manner both on the hepatoma cell line and on the human SR-BI-transfected cell line by an anti-HVR1 monoclonal antibody...
  61. pmc Lung cancer susceptibility locus at 5p15.33
    James D McKay
    International Agency for Research on Cancer IARC, Lyon, France
    Nat Genet 40:1404-6. 2008
    ..016). The susceptibility region contains two genes, TERT and CLPTM1L, suggesting that one or both may have a role in lung cancer etiology...
  62. ncbi FoxO3 controls autophagy in skeletal muscle in vivo
    Cristina Mammucari
    Venetian Institute of Molecular Medicine, 35129 Padova, Italy
    Cell Metab 6:458-71. 2007
    ..These findings point to FoxO3 and Bnip3 as potential therapeutic targets in muscle wasting disorders and other degenerative and neoplastic diseases in which autophagy is involved...
  63. pmc Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways
    Liyun Sang
    Genentech Inc, South San Francisco, CA 94080, USA
    Cell 145:513-28. 2011
    ..Our study further illustrates the power of linking proteomic networks and human genetics to uncover critical disease pathways...
  64. pmc Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes
    Birke Bartosch
    Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, Institut National de la Santé et de la Recherche Médicale U412, IFR 128, Ecole Normale Superieure de Lyon, 69364 Lyon Cedex 07, France
    J Exp Med 197:633-42. 2003
    ..Altogether, these studies indicate that these pseudo-particles may mimic the early infection steps of parental HCV and will be suitable for the development of much needed new antiviral therapies...
  65. ncbi Secreted antagonists of the Wnt signalling pathway
    Yoshiaki Kawano
    Department of Cancer Cell Biology, Division of Medicine, Imperial College, London W12 0NN, UK
    J Cell Sci 116:2627-34. 2003
    ..In addition, there are other protein interactions that contribute to Wnt antagonist function. Moreover, certain sFRPs and Dkks do not antagonise Wnt function, which suggests that these families have as-yet-undiscovered functions...
  66. ncbi Notch-mediated restoration of regenerative potential to aged muscle
    Irina M Conboy
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305 5235, USA
    Science 302:1575-7. 2003
    ..Thus, Notch signaling is a key determinant of muscle regenerative potential that declines with age...
  67. pmc Orchestrating the unfolded protein response in health and disease
    Randal J Kaufman
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
    J Clin Invest 110:1389-98. 2002
  68. ncbi Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes
    Umut Ozcan
    Department of Genetics and Complex Diseases, Harvard Medical School, Boston, MA 02115, USA
    Science 306:457-61. 2004
    ..Pharmacologic manipulation of this pathway may offer novel opportunities for treating these common diseases...
  69. doi TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity
    Antonella Caputo
    Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova 16148, Italy
    Science 322:590-4. 2008
    ..The molecular identity of these membrane proteins is still unclear...
  70. pmc IFI16 is an innate immune sensor for intracellular DNA
    Leonie Unterholzner
    School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Nat Immunol 11:997-1004. 2010
    ..IFI16 (p204) is the first PYHIN protein to our knowledge shown to be involved in IFN-β induction. Thus, the PYHIN proteins IFI16 and AIM2 form a new family of innate DNA sensors we call 'AIM2-like receptors' (ALRs)...
  71. pmc STING regulates intracellular DNA-mediated, type I interferon-dependent innate immunity
    Hiroki Ishikawa
    Department of Medicine and Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    Nature 461:788-92. 2009
    ..Collectively, our studies indicate that STING is essential for host defence against DNA pathogens such as HSV-1 and facilitates the adjuvant activity of DNA-based vaccines...
  72. ncbi HES and HERP families: multiple effectors of the Notch signaling pathway
    Tatsuya Iso
    Institute for Genetic Medicine, Department of Biochemistry and Molecular Biology, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089, USA
    J Cell Physiol 194:237-55. 2003
    ..The identification of the HERP family as a Notch effector that cooperates with HES/E(spl) family has opened a new avenue to our understanding of the Notch signaling pathway...
  73. pmc Identification and proteomic profiling of exosomes in human urine
    Trairak Pisitkun
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Proc Natl Acad Sci U S A 101:13368-73. 2004
    ..The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine...
  74. ncbi Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis
    Mats Hellstrom
    AngioGenetics Sweden AB, Scheeles vag 2, SE 171 77 Stockholm, Sweden
    Nature 445:776-80. 2007
    ....
  75. pmc Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA
    Jiaxi Wu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 339:826-30. 2013
    ..cGAMP bound to STING, leading to the activation of IRF3 and induction of interferon-β. Thus, cGAMP functions as an endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA...
  76. pmc Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice
    Takehiro Nitta
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Cell Biol 161:653-60. 2003
    ..e., the size-selective loosening of the BBB) not only provides new insight into the basic molecular physiology of BBB but also opens a new way to deliver potential drugs across the BBB into the central nervous system...
  77. ncbi Rho GTPases and the actin cytoskeleton
    A Hall
    Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research Campaign Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Science 279:509-14. 1998
    ....
  78. pmc DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL
    Mikhail B Litinskiy
    Division of Molecular Immunology, Department of Pathology and Laboratory Medicine, Joan and Sanford I Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nat Immunol 3:822-9. 2002
    ..By eliciting CD40-independent Ig class switching and plasmacytoid differentiation, BLyS and APRIL critically link the innate and adaptive immune responses...
  79. pmc The branching programme of mouse lung development
    Ross J Metzger
    Department of Biochemistry and HHMI, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nature 453:745-50. 2008
    ..We show that this hierarchical and modular programme is genetically tractable, and it is ideally suited to encoding and evolving the complex networks of the lung and other branched organs...
  80. pmc Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms
    Jessica M Weidner
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 84:12646-57. 2010
    ..of this well-studied virus infection system, we show that although both tetherin and IFITM3 are plasma membrane proteins, tetherin inhibits virion particle release from infected cells, while IFITM3 disrupts an early event after ..
  81. ncbi The glucose-regulated proteins: stress induction and clinical applications
    A S Lee
    University of Southern California Norris Comprehensive Cancer Center, Dept of Biochemistry and Molecular Biology, University of Southern California Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089 9176, USA
    Trends Biochem Sci 26:504-10. 2001
    ..The elucidation of Grp function and regulation opens up new therapeutic approaches to diseases associated with ER stress and cancer...
  82. pmc The PINK1/Parkin pathway regulates mitochondrial morphology
    Angela C Poole
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 105:1638-43. 2008
    ..Our studies suggest that the PINK1/Parkin pathway promotes mitochondrial fission and that the loss of mitochondrial and tissue integrity in PINK1 and parkin mutants derives from reduced mitochondrial fission...
  83. pmc Collaborative induction of inflammatory responses by dectin-1 and Toll-like receptor 2
    Benjamin N Gantner
    Department of Immunology, University of Washington, Seattle, 98103, USA
    J Exp Med 197:1107-17. 2003
    ..The data demonstrate that collaborative recognition of distinct microbial components by different classes of innate immune receptors is crucial in orchestrating inflammatory responses...
  84. pmc Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene
    Xueping Qu
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Clin Invest 112:1809-20. 2003
    ..Thus, mutation of beclin 1 or other autophagy genes may contribute to the pathogenesis of human cancers...
  85. ncbi Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease
    Laurent Monney
    Center for Neurologic Diseases, Department of Neurology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 415:536-41. 2002
    ..Tim-3 may have an important role in the induction of autoimmune diseases by regulating macrophage activation and/or function...
  86. pmc Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo
    Amanda M Marchiando
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    J Cell Biol 189:111-26. 2010
    ..These data demonstrate that MLCK activation triggers caveolin-1-dependent endocytosis of occludin to effect structural and functional tight junction regulation...
  87. ncbi A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol
    Yihong Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 429:841-7. 2004
    ..Derlin-1 interacts with US11, a virally encoded ER protein that specifically targets MHC class I heavy chains for export from the ER, as well as with VIMP, a novel membrane protein that recruits the p97 ATPase and its cofactor...
  88. ncbi The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor
    M S Brown
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 89:331-40. 1997
  89. doi The blood-brain barrier in health and chronic neurodegenerative disorders
    Berislav V Zlokovic
    Center for Neurodegenerative and Vascular Brain Disorders, Frank P Smith Laboratory for Neuroscience and Neurosurgical Research, University of Rochester Medical School, Rochester, NY 14642, USA
    Neuron 57:178-201. 2008
    ..These findings support developments of new therapeutic approaches for chronic neurodegenerative disorders directed at the BBB and other nonneuronal cells of the neurovascular unit...
  90. ncbi Dysferlin, a novel skeletal muscle gene, is mutated in Miyoshi myopathy and limb girdle muscular dystrophy
    J Liu
    Day Neuromuscular Research Laboratory, Charlestown, Massachusetts 02129, USA
    Nat Genet 20:31-6. 1998
    ..Identical mutations in the dysferlin gene can produce more than one myopathy phenotype (MM, limb girdle dystrophy, distal myopathy with anterior tibial onset)...
  91. pmc Role of BNIP3 and NIX in cell death, autophagy, and mitophagy
    J Zhang
    Department of Biochemistry, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38117 3678, USA
    Cell Death Differ 16:939-46. 2009
    ..In this review, we will discuss possible mechanisms by which BNIP3 and NIX induce cell death and mitophagy. We will also consider the potential relationship between cell death pathways and autophagy in development and homeostasis...
  92. pmc Interaction between Plasmodium falciparum apical membrane antigen 1 and the rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites
    Dave Richard
    Walter and Eliza Hall Institute of Medical Research, Parkville, Melbourne, Victoria 3052, Australia
    J Biol Chem 285:14815-22. 2010
    ..We propose that the formation of the AMA1-RON complex is essential for secretion of the rhoptry contents, which then allows the establishment of parasite infection within the parasitophorous vacuole...
  93. pmc Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles
    Mayla Hsu
    Aaron Diamond AIDS Research Center and Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:7271-6. 2003
    ..However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex...
  94. ncbi p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
    A Yang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 398:714-8. 1999
    ..Taken together, our results indicate that p63 is critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis...
  95. ncbi GROMACS: fast, flexible, and free
    David van der Spoel
    Department of Cell and Molecular Biology, Uppsala University, Husargatan 3, Box 596, S 75124 Uppsala, Sweden
    J Comput Chem 26:1701-18. 2005
    ..It is maintained by a group of developers from the Universities of Groningen, Uppsala, and Stockholm, and the Max Planck Institute for Polymer Research in Mainz. Its Web site is http://www.gromacs.org...
  96. ncbi Translocation of proteins into mitochondria
    Walter Neupert
    Institut fur Physiologische Chemie, Universitat Munchen, 81377 Munchen, Germany
    Annu Rev Biochem 76:723-49. 2007
    ..This review summarizes the present knowledge on the import and sorting of mitochondrial precursor proteins, with a special emphasis on unresolved questions and topics of current research...
  97. ncbi Alzheimer's disease is a synaptic failure
    Dennis J Selkoe
    Center for Neurologic Diseases, Brigham and Women s Hospital, and the Harvard Center for Neurodegeneration and Repair, Boston, MA 02115, USA
    Science 298:789-91. 2002
    ....
  98. pmc Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development
    Hsiuchen Chen
    Division of Biology, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 160:189-200. 2003
    ..Therefore, mitochondrial fusion is essential for embryonic development, and by enabling cooperation between mitochondria, has protective effects on the mitochondrial population...
  99. pmc TOPCONS: consensus prediction of membrane protein topology
    Andreas Bernsel
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91, Sweden
    Nucleic Acids Res 37:W465-8. 2009
    ..The web interface allows for constraining parts of the sequence to a known inside/outside location, and detailed results are displayed both graphically and in text format...
  100. pmc The beta-glucan receptor dectin-1 functions together with TLR2 to mediate macrophage activation by mycobacteria
    Mahesh Yadav
    Department of Biological Sciences, Center for Tropical Disease Research and Training, University of Notre Dame, 130 Galvin Life Science Center, Notre Dame, IN 46556, USA
    Blood 108:3168-75. 2006
    ..These are the first results to establish a significant role for dectin-1, in cooperation with TLR2, to activate a macrophage's proinflammatory response to a mycobacterial infection...
  101. pmc Estimating medium- and long-term trends in malaria transmission by using serological markers of malaria exposure
    C J Drakeley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 102:5108-13. 2005
    ..Thus, serological tools allow us to detect variations in malaria transmission over time. Such tools will be invaluable for monitoring trends in malaria endemicity and the effectiveness of malaria control programs...

Research Grants78

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..Based upon recent evidence, we propose that membrane proteins are concentrated in "protein islands," protein and cholesterol-rich domains separated by expanses of mostly ..
  2. Structure and function of urea transporters
    Ming Zhou; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Urea transporters (UT) are integral membrane proteins that facilitate transport of urea across cell membrane. UTs are highly expressed in many mammalian tissues, including kidney, liver, and brain...
  3. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
    ..We have observed that mutants in key membrane proteins such as dynamin and dystroglycan phenocopy the Sply myopathy...
  4. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    ..Nedd4-2 induces down regulation of a number of membrane proteins including ENaC;this results in reduced sodium reabsorption...
  5. Role for Na,K-ATPase in Nucleoplasmic Calcium Homeostasis
    Craig Gatto; Fiscal Year: 2010
    ....
  6. REGULATION OF PULMONARY CIRCULATION IN FETUS AND NEWBORN
    Girija G Konduri; Fiscal Year: 2013
    ..These studies will identify an important new source of oxidative stress in PPHN. These observation may lead to new targeted therapies to improve vasodilation and oxygenation in PPHN. ..
  7. Identification of Proteins Important for Male Osteoporosis
    Hong Wen Deng; Fiscal Year: 2013
    ..proteins and proteins with extreme isoelectric point, molecular weight, and hydrophobicity, especially membrane proteins. A NOVEL approach to this problem is subcellular proteome extraction to stepwise isolate proteins from ..
  8. Molecular Biology of Human Erythrocyte Alpha Spectrin
    Patrick G Gallagher; Fiscal Year: 2013
    ..Some people require surgery or blood transfusions to treat the anemia. This application studies the what, why, and how of abnormalities of the lining of the red blood cell that cause the anemia. ..
  9. Liver disease in CF: CFTR controls innate immunity in biliary epithelium
    Mario Strazzabosco; Fiscal Year: 2013
    ....
  10. Inflammatory hyperalgesia due to TRPV1, the pepper spray receptor in the cornea
    Sharona E Gordon; Fiscal Year: 2013
    ..Although cell surface receptor-stimulated trafficking of membrane lipids and membrane proteins is of broad significance to biology, the molecular mechanisms by which it occurs are poorly understood...
  11. Endoplasmic Reticulum Chaperone as a Regulator of Obesity and Diabetes
    Amy S Lee; Fiscal Year: 2010
    ..If validated, our findings may lead to new targets for therapy against obesity and type 2 diabetes in humans. ..
  12. The biology of blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2013
    ..I. will examine the hypothesis that TNF? regulates BTB dynamics via its effects on basement membrane proteins, such as collagens, proteases and protease inhibitors, which, in turn, regulates the steady-state levels of ..
  13. Leptin as a Regulator of T Cell Metabolism and Function
    NANCIE MACIVER; Fiscal Year: 2013
    ..The hormone leptin, which is secreted by fat cells in proportion to fat mass and affects immune function, may be responsible for linking nutritional status with immune cell function. ..
  14. Inhibitors of the Y. pestis cell surface plasminogen activator for biodefense
    Marjorie H Barnes; Fiscal Year: 2010
    ..These new drugs will not be susceptible to existing resistance mechanisms and will provide a proof of principle for the efficacy of this therapeutic approach. ..
  15. Regulation of Signaling Receptor Activity by LRP-1
    Steven L Gonias; Fiscal Year: 2013
    ..is a transmembrane receptor that mediates the endocytosis of over forty distinct ligands and other plasma membrane proteins. LRP-1 also regulates cell-signaling by two distinct mechanisms...
  16. Development of a novel method for inhibiting diabetic retinopathy
    David R Clemmons; Fiscal Year: 2012
    ..The work in this proposal will determine if this approach is feasible using animal models of this disease state. ..
  17. Development of Bioactive Chemical Probes for Calcium-activated Chloride Channel
    Min Li; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Ion channels are membrane proteins, found in both excitable cells and non-excitable cells...
  18. Phospholipid flip-flop in biogenic membranes
    Anant K Menon; Fiscal Year: 2013
    ..purification and mass spectrometry on the one hand, and screening of systematic collections of yeast ER membrane proteins on the other. Our purification efforts will be aided by the use of novel affinity matrices...
  19. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..These labeled membrane proteins will be identified by mass spectrometry and will represent possible thiol isomerase substrates...
  20. Developmental Regulation of Brain and Behavior
    JULI S WADE; Fiscal Year: 2011
    ....
  21. REGULATION OF NUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY
    Katherine L Wilson; Fiscal Year: 2012
    ABSTRACT The functions of nuclear membrane proteins and A-type lamin filaments, linked to a growing number of signaling pathways, human diseases (`laminopathies') and human aging, are an important open frontier in biology...
  22. Matrix Otopathology
    MICHAEL ANNE NMI GRATTON; Fiscal Year: 2011
    ..2) The accumulation of basement membrane proteins upregulate hypoxia-related responses that modulate genes associated with basement membrane homeostasis...
  23. Developing Immunotherapeutics for Methamphetamine Abuse
    THOMAS RICHARD KOSTEN; Fiscal Year: 2010
    ..Orson and Kinsey have been working on the development of DMA vaccines. Our group's combined experience is ideally positioned to get a vaccine quickly into phase 1 human studies. ..
  24. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
    ....
  25. Genetically testing interactions of ER and oxidative stresses in retinal disease
    Douglas Gould; Fiscal Year: 2013
    ..Knowledge of the relative roles of ER and oxidative stress can be exploited in development of targeted therapeutics that could prevent or reduce vision loss in millions of people worldwide. ..
  26. Melanopsin Signal Transduction Studied by FTIR Spectroscopy
    Kenneth J Rothschild; Fiscal Year: 2012
    ..recent development of methods to: i) measure sub-picosecond protein changes;ii) probe minute quantities of membrane proteins including single crystals using time-resolved FTIR microscopy and iii) rapidly in vitro express membrane ..
  27. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    Pei Zhou; Fiscal Year: 2012
    ..Subsequent trafficking of nascent LPS to the outer membrane involves a second ABC transporter and two outer membrane proteins, encoded by the lpt genes...
  28. Phospholemman and Cardiac Contracility
    Joseph Y Cheung; Fiscal Year: 2013
    ..DESCRIPTION (provided by applicant): Phospholemman (PLM), the 1st member of the FXYD family of small integral membrane proteins involved in regulation of ion transport, modulates the function of both Na+-K+-ATPase and Na+/Ca2+ exchanger ..
  29. INTERNATIONAL HERPESVIRUS WORKSHOP
    Jay A Nelson; Fiscal Year: 2012
    ..approach to the following topics: virus structure, mechanism of virus entry and cell-cell spread, membrane proteins, pathogenesis and latency, DNA replication, vaccination and the immune response, transcriptional control, ..
  30. The Roles of Conserved outer-membrane proteins in SFG rickettsia pathogenesis
    Juan J Martinez; Fiscal Year: 2013
    ....
  31. Liver Cell Membrane Protein-Expression and Function
    Allan W Wolkoff; Fiscal Year: 2012
    This is a Program Project to continue investigation of the functions and trafficking of specific liver cell membrane proteins. Four projects involving 15 faculty investigators representing 6 academic departments are proposed...
  32. Molecular pathogenesis and genetic diversification of childhood falciparum malari
    STEVE MYER TAYLOR; Fiscal Year: 2013
    ..abstract_text> ..
  33. Molecular mechanisms of bacterial homologs of neurotransmitter:sodium symporters
    Jonathan A Javitch; Fiscal Year: 2013
    ..abstract_text> ..
  34. A Molecular targeting agent for systemic radiotherapy of metastatic Prostate Canc
    Kevin P Maresca; Fiscal Year: 2010
    ..This proposal is designed to generate the pre-clinical efficacy data for the corresponding therapeutic I-131-MIP-1072 in order to assess the possibilities of developing the imaging agent into an effective treatment for prostate cancer. ..
  35. Vaccines for Sustainable Therapy of Opiate Addiction
    Frank M Orson; Fiscal Year: 2012
    ....
  36. 2011 Mechanisms of Membrane Transport Gordon Research Conference
    Nancy Carrasco; Fiscal Year: 2011
    ..Recent success in obtaining high-resolution structures of membrane proteins from model organisms has inspired the field and encouraged entry of a new generation of investigators...
  37. FUNCTION OF THE RAS RELATED RAL PROTEINS
    LARRY FEIG; Fiscal Year: 2009
    ..unique set of downstream signaling molecules that regulate multiple cellular processes including delivery of membrane proteins like E-cadherin to the basolateral plasma membrane of epithelial cells, exocytosis, apoptosis, cell ..
  38. HEREDITARY DEFECTS IN HUMAN SODIUM CHANNELS
    Alfred L George; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Voltage-gated sodium (NaV) channels are heteromultimeric integral membrane proteins that are responsible for the initial phase of the action potential in most excitable cells...
  39. Function of mRNA Editing in Trypanosomes
    Stephen L Hajduk; Fiscal Year: 2010
    ..Together these studies will provide the first analysis of the role of RNA editing in protein diversification in trypanosomes and is likely to lead to the discovery of novel mitochondrial proteins. ..
  40. Novel Erythroid Cell Membrane Protein
    Emery H Bresnick; Fiscal Year: 2010
    ..mFam55B appears to be a Type II membrane protein, whereas ICAMs are Type I membrane proteins. We discovered four mammalian mFam55B-related genes, which have not been described, but only mFam55B is GATA-..
  41. Alterations in membrane composition and function eith calorie restriction
    Jon J Ramsey; Fiscal Year: 2011
    ..Also, these theories ignore the role membrane proteins play in protecting against oxidative damage...
  42. DC-SIGN Inhibitors for the Treatment of HIV Infection
    ALLEN BERNARD REITZ; Fiscal Year: 2013
    ....
  43. Problems in Membrane Protein Crystallography: Hetero-Oligomeric Cytochrome b6f
    William A Cramer; Fiscal Year: 2012
    ..Such membranes contain the majority of the relatively few hetero-oligomeric integral membrane proteins that have been solved by X-ray crystallography to a resolution d 3.0 E...
  44. INTERNATIONAL HERPESVIRUS WORKSHOP
    Jay A Nelson; Fiscal Year: 2011
    ..approach to the following topics: virus structure, mechanism of virus entry and cell-cell spread, membrane proteins, pathogenesis and latency, DNA replication, vaccination and the immune response, transcriptional control, ..
  45. Biology of the HMW1 and HMW2 Adhesins of H. Influenzae
    JOSEPH W ST. GEME; Fiscal Year: 2013
    ..influenzae disease. More generally, these studies will provide fundamental insights into host-microbial relationships, bacterial protein secretion, and prokaryotic protein glycosylation. ..
  46. Association of a common variant of the PPAP2B gene with cardiovascular disease.
    Andrew J Morris; Fiscal Year: 2013
    ..Establishing PPAP2B, its product LPP3 and the lipid substrates of this enzyme as risk predictors and mediators of CAD promises to identify important and innovative targets for the development of new biomarkers and/or therapeutics. ..
  47. Identification of a Molecular Signature for Barrier Insulators
    LAURIE ANN STEINER; Fiscal Year: 2013
    ..The goal of this application is to study the location, structure, and function of barrier insulators in mammalian cells, so that we can better understand this fundamental mechanism of gene regulation. ..
  48. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    Alexander A Spector; Fiscal Year: 2012
    ..Prestin belongs to a family of membrane proteins that facilitate the movement of anions (negatively charged ions such as chloride) through the cell membrane...
  49. Chemistry, Structure and Biology of Thiol Transferases
    Richard N Armstrong; Fiscal Year: 2013
    ..All of these proteins are integral membrane proteins. Specific Aim 1 relies heavily on hydrogen/deuterium exchange mass spectrometry (H/DEX MS) to probe the ..
  50. Gonococcal Mechanisms to Evade Host Defenses
    WILLIAM MAURICE SHAFER; Fiscal Year: 2013
    ..We hypothesize that genes involved in lipooligosaccharide (LOS) biosynthesis and those that encode membrane proteins are important in determining the ability of gonococci to resist the bactericidal action of SAC...
  51. CD-73 adenosine signaling in the central nervous system in disease and health
    Margaret S Bynoe; Fiscal Year: 2012
    ..Such therapies could be used to stop the progress of, or even possibly reverse the damage caused by MS. ..
  52. The Molecular Basis of Allorecognition in Social Amoeba
    Gad Shaulsky; Fiscal Year: 2013
    ..We found these trans-membrane proteins to be highly polymorphic in natural Dictyostelium populations and necessary and sufficient for ..
  53. Structure and Specificity in Metal Ion Homeostasis
    Amy C Rosenzweig; Fiscal Year: 2013
    ..The P1B-type ATPases, integral membrane proteins that use the energy of ATP hydrolysis to transport metal ions across membranes, play a key role in metal ..
  54. EFFECT OF R1R2 OVER-EXPRESSION ON CARDIAC FUNCTION
    Michael Regnier; Fiscal Year: 2013
    ..Mechanistic interpretations will be aided by proteomic analysis of myofilament and membrane proteins to assess isoform and phosphorylation profiles...
  55. Genetic Analysis of Mouse Nervous System Development
    Kathryn V Anderson; Fiscal Year: 2013
    ..These experiments will define the roles of cilia ad centrosomes in the control asymmetric cell division and migration in the cortex. ..
  56. Key determinants of dengue virus neutralization by naturally occurring human mAbs
    Scott A Smith; Fiscal Year: 2013
    ..These skills, along with didactic coursework, will help me to develop my career, as a physician-scientist, in a direction independently of my mentor. ..
  57. 2010 and 2012 Membrane Transport Proteins
    Robert H Edwards; Fiscal Year: 2012
    ..However, technical limitations associated with the analysis of membrane proteins and transporters in particular has previously limited progress in our understanding of these fascinating and ..
  58. Disruption of Extracellular Matrix-Glial Interactions in Schizophrenia
    Sabina Berretta; Fiscal Year: 2013
    ..will be used in Specific Aim 4 to test dynamically the potential of growth factors and secretory carrier membrane proteins, investigated in Specific Aim 2, to cause CSPG accumulation in human astrocytes and decreased CSPG secretion ..
  59. Roles of Epstein-Barr virus nuclear antigens 2 and LP in B cell proliferation
    Elliott D Kieff; Fiscal Year: 2013
    ....
  60. Adiporedoxin, a Novel Player in Adipokine Secretion
    Paul F Pilch; Fiscal Year: 2013
    ..By understanding the mechanism of Arx action, these consequences can be addressed by appropriate therapeutic strategies. ..
  61. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..deficiencies occur in genes for Dol-P-sugar-utilizing glycosyltransferases (DPS-GT), unusual multi-spanning membrane proteins. The mechanisms of DPS-GT functions are unknown, so the effects of disease-causing mutations are obscure...
  62. Receptor-mediated Endocytosis-Mechanism and Function
    Frederick R Maxfield; Fiscal Year: 2013
    ..responsible for lipid transport, and membrane lipids strongly Influence the transport and distribution of membrane proteins. In Aim I the role of specific proteins will be studied using rapid and selective protein inactivation by ..
  63. Membrane-associated PDE3-binding proteins in human myocardium
    Matthew A Movsesian; Fiscal Year: 2013
    ..The research proposed in this application, which focuses on a novel molecular mechanism through which a new pharmacological strategy for the treatment of heart failure can be based, has the potential to lead to such improvements. ..
  64. Cholesterol and Sphingolipid Perturbation of Membrane Traffic in Human Disease
    Andrew H Limper; Fiscal Year: 2011
    ..since these lipids can significantly affect membrane transport as well as the microenvironment of other membrane proteins. Based on preliminary data showing reduced cilia in ?F508-CFTR cells, we will also study the effect of ..
  65. CELLULAR MECHANISMS OF PTLD IN TRANSPLANT RECIPIENTS
    Olivia M Martinez; Fiscal Year: 2012
    ..Thus, studies that identify and define novel targets for inhibiting tumor growth could lead to new diagnostic and therapeutic agents for prevention of this devastating disease. ..
  66. Development of an Algae-based Membrane Protein Expression System
    ALEXANDER ARAVANIS; Fiscal Year: 2009
    ..capable of producing relatively large amounts of highly purified prokaryotic and eukaryotic integral membrane proteins. Completion of Phase II work would represent validation of a new expression system that uses the chloroplast ..
  67. Impact of Oral bacterial interactions on periodontitis
    Eric Reynolds; Fiscal Year: 2004
    ..Further, outer membrane proteins that are altered during this interaction also have not been studied...
  68. Molecular basis of congenital disorders of glycosylation: mechanisms of oligosach
    Smita Mohanty; Fiscal Year: 2010
    ..Despite the fact that 30% of all genes encode membrane proteins and over 50% of drug targets are a class of membrane proteins called G protein-coupled receptors (GPCRs), ..
  69. Apparatus for encapsulating integral membrane proteins for structural studies by
    RONALD WILLIAM PETERSON; Fiscal Year: 2010
    ..Approximately half of current drug targets are integral membrane proteins, yet as the number of known structures approaches 50,000 only a few hundred of those are of integral ..
  70. Hemolytic Anemia: Biochemical, Molecular and Proteomic Diagnostics
    Jeffrey S Friedman; Fiscal Year: 2010
    ..We are pursuing these studies to try to find the cause of this disorder for the large group of patients for whom we can currently provide no specific diagnosis. ..
  71. 3-D CRYSTALLIZATION OF MEMBRANE PROTEINS
    JAMES GOUAUX; Fiscal Year: 1999
    ..bind to tertiary epitopes on the membrane protein complexes, to carry out crystallization trials on the membrane proteins, alone and in combination with Fv domains, and to analyze the resulting crystals by x-ray diffraction...
  72. Anachem Lipidic Cubic Phase Crystallization Robot
    Robert M Stroud; Fiscal Year: 2010
    ..Membrane Protein Crystallization and Structure Determination in Lipidic Cubic Phase or Sponge Mesophase: Membrane proteins constitute 30% of the genome, and are targets for over 40% of all drugs in use today, however only in the ..
  73. Crystallization of His-tag Proteins on Nanostructured 1D & 2D Template Interface
    David Thompson; Fiscal Year: 2007
    ..obtain high-quality 1D and 2D crystals of histidine-tagged (His-tag) soluble proteins & His-tag integral membrane proteins (IMP) using a family of new nitrilotriacetic acid (NTA) reagents that promote nucleation in a symmetry-..
  74. Membrane Proteins of S. mansoni as Vaccine Candidates
    Donald Harn; Fiscal Year: 2002
    ..performing functions such as nutrient uptake and attachment that are normally performed by integral surface membrane proteins, the parasites must have developed mechanisms for avoiding immune attack directed at these proteins...
  75. Structure-Function of Hetero-oligomeric Integral Membrane Proteins
    WILLIAM CRAMER; Fiscal Year: 2007
    ..The cytochrome b6f complex is one of a small number (presently, 17) of hetero-oligomeric integral membrane proteins whose crystal structure has been solved to a resolution better than 3.0 A...
  76. Eukaryotic Membrane Protein Folding in E. coli
    John Spudich; Fiscal Year: 2006
    Heterologous expression of correctly folded eukaryotic membrane proteins is currently the primary limitation to their structure/function study...
  77. Innovative Methods for Membrane Protein Crystallization
    PAUL KENIS; Fiscal Year: 2006
    ..and methodology to substantially speed up the process of determining optimal crystallization conditions for membrane proteins. The crystallization platform will enable precise control of the path towards supersaturation, to identify ..
  78. Droplet Method for Bilayer Assays
    Gregory W Faris; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Membrane proteins are extremely important to understanding human health and treating disease...