green fluorescent proteins

Summary

Summary: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.

Top Publications

  1. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
  2. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
  3. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
  4. ncbi A global double-fluorescent Cre reporter mouse
    Mandar Deepak Muzumdar
    Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA
    Genesis 45:593-605. 2007
  5. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
  6. pmc Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion
    S Jung
    Skirball Institute of Biomolecular Medicine and Howard Hughes Medical Institute New York University Medical Center, New York, New York 10016, USA
    Mol Cell Biol 20:4106-14. 2000
  7. ncbi Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP
    G Feng
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 28:41-51. 2000
  8. ncbi A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function
    John D Cahoy
    Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Neurosci 28:264-78. 2008
  9. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
  10. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007

Detail Information

Publications326 found, 100 shown here

  1. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
    ..Thus, these plasmids should further facilitate the rapid analysis of gene function in S. cerevisiae...
  2. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  3. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
    ..Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells...
  4. ncbi A global double-fluorescent Cre reporter mouse
    Mandar Deepak Muzumdar
    Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA
    Genesis 45:593-605. 2007
    ..In addition to serving as a global Cre reporter, the mT/mG mouse may also be used as a tool for lineage tracing, transplantation studies, and analysis of cell morphology in vivo...
  5. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
    ..When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules...
  6. pmc Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion
    S Jung
    Skirball Institute of Biomolecular Medicine and Howard Hughes Medical Institute New York University Medical Center, New York, New York 10016, USA
    Mol Cell Biol 20:4106-14. 2000
    ..Furthermore, a prominent response of CX(3)CR1-deficient microglia to peripheral nerve injury indicates unimpaired neuronal-glial cross talk in the absence of CX(3)CR1...
  7. ncbi Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP
    G Feng
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 28:41-51. 2000
    ..In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal...
  8. ncbi A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function
    John D Cahoy
    Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Neurosci 28:264-78. 2008
    ....
  9. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
    ..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community...
  10. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
    ..This method will be of general utility for analysis of the autophagosome maturation process...
  11. ncbi In vivo imaging of embryonic vascular development using transgenic zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 248:307-18. 2002
    ....
  12. ncbi The green fluorescent protein
    R Y Tsien
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647, USA
    Annu Rev Biochem 67:509-44. 1998
    ..Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories...
  13. pmc Single-copy insertion of transgenes in Caenorhabditis elegans
    Christian Frøkjaer-Jensen
    Department of Biology and Howard Hughes Medical Institute, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
    Nat Genet 40:1375-83. 2008
    ..We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines...
  14. ncbi Spatiotemporal rescue of memory dysfunction in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 302:1765-8. 2003
    ..The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required...
  15. pmc Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
    Samuel T Hess
    Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
    Biophys J 91:4258-72. 2006
    ..This new method suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution...
  16. ncbi 'Green mice' as a source of ubiquitous green cells
    M Okabe
    Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEBS Lett 407:313-9. 1997
    ..The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments...
  17. ncbi Engineering and characterization of a superfolder green fluorescent protein
    Jean Denis Pédelacq
    Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Nat Biotechnol 24:79-88. 2006
    ..X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP...
  18. ncbi Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
    Carlos Lois
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:868-72. 2002
    ..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
  19. ncbi Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
    Tsuyoshi Nakagawa
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 1060 Nishikawatsu, Matsue, Japan
    J Biosci Bioeng 104:34-41. 2007
    ..Moreover, tissue-specific and inducible gene expression using a promoter was also monitored with pGWBs. It is expected that, the pGWB system will serve as a powerful tool for plasmid construction in plant research...
  20. ncbi Directed differentiation of embryonic stem cells into motor neurons
    Hynek Wichterle
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Cell 110:385-97. 2002
    ..Thus, inductive signals involved in normal pathways of neurogenesis can direct ES cells to form specific classes of CNS neurons...
  21. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
  22. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  23. pmc Improving the photostability of bright monomeric orange and red fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nat Methods 5:545-51. 2008
    ..and TagRFP (a monomeric derivative of eqFP578 from Entacmaea quadricolor) that maintain most of the beneficial qualities of the original proteins and perform as reliably as Aequorea victoria GFP derivatives in fusion constructs...
  24. pmc An expanded palette of genetically encoded Ca²⁺ indicators
    Yongxin Zhao
    Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
    Science 333:1888-91. 2011
    ..This palette of indicators paints the way to a colorful new era of Ca(2+) imaging...
  25. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
    ..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
  26. ncbi FACS-optimized mutants of the green fluorescent protein (GFP)
    B P Cormack
    Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
    Gene 173:33-8. 1996
    ..coli, the folding of the mutant proteins is more efficient than folding of wt GFP. These two properties contribute to a greatly increased (100-fold) fluorescence intensity, making the mutants useful for a number of applications...
  27. ncbi Bone marrow cells regenerate infarcted myocardium
    D Orlic
    Hematopoiesis Section, Genetics and Molecular Biology Branch, NHGRI, NIH, Bethesda, MD 20892, USA
    Nature 410:701-5. 2001
    ..The developing tissue comprised proliferating myocytes and vascular structures. Our studies indicate that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease...
  28. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..Thus FRET between GFP mutants can monitor localized Ca2+ signals and protein heterodimerization in individual live cells...
  29. ncbi Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse
    Nobuaki Tamamaki
    Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Comp Neurol 467:60-79. 2003
    ..4% of the GFP-positive cells. They accounted for 6.5% in layer I. In accord with unidentified GFP-positive cells, many medium-sized spherical somata emitting intense GFP fluorescence were observed in layer I...
  30. ncbi The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs
    Kristen M Kwan
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah, USA
    Dev Dyn 236:3088-99. 2007
    ..The Tol2kit greatly facilitates zebrafish transgenesis, simplifies the sharing of clones, and enables large-scale projects testing the functions of libraries of regulatory or coding sequences...
  31. ncbi Green fluorescent protein as a marker for gene expression
    M Chalfie
    Department of Biological Sciences, Columbia University, New York, NY 10027
    Science 263:802-5. 1994
    ..Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms...
  32. ncbi A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins
    Ulrich Rothbauer
    Munich Center for Integrated Protein Science, CiPS M and Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    Mol Cell Proteomics 7:282-9. 2008
    b>Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization and dynamics...
  33. ncbi Functional neurogenesis in the adult hippocampus
    Henriette van Praag
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 415:1030-4. 2002
    ..Our findings demonstrate that newly generated cells mature into functional neurons in the adult mammalian brain...
  34. pmc Automatic and quantitative measurement of protein-protein colocalization in live cells
    Sylvain V Costes
    Image Analysis Laboratory, National Cancer Institute, Frederick, Maryland, USA
    Biophys J 86:3993-4003. 2004
    ..25 x 10(-3) s(-1). More generally, this algorithm can be used to answer a variety of biological questions involving protein-protein interactions or co-compartmentalization and can be generalized to colocalization of more than two colors...
  35. pmc Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes
    Kevin D Foust
    Center for Gene Therapy, The Research Institute at Nationwide Children s Hospital, 700 Children s Drive, Columbus, Ohio 43205, USA
    Nat Biotechnol 27:59-65. 2009
    ..It may also be useful for rapid postnatal genetic manipulations in basic neuroscience studies...
  36. ncbi Local, efflux-dependent auxin gradients as a common module for plant organ formation
    Eva Benkova
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Cell 115:591-602. 2003
    ..Our results suggest that PIN-dependent, local auxin gradients represent a common module for formation of all plant organs, regardless of their mature morphology or developmental origin...
  37. ncbi A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein
    J Nakai
    Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki, 444 8585, Japan
    Nat Biotechnol 19:137-41. 2001
    ..G-CaMP will be a useful tool for visualizing intracellular Ca2+ in living cells. Mutational analysis, together with previous structural information, suggests the residues that may alter the fluorescence of GFP...
  38. ncbi An improved zinc-finger nuclease architecture for highly specific genome editing
    Jeffrey C Miller
    Sangamo BioSciences, Inc, Pt Richmond Tech Center, 501 Canal Blvd, Suite A100 Richmond, California 94804, USA
    Nat Biotechnol 25:778-85. 2007
    ..This architecture provides a general means for improving the specificity of ZFNs as gene modification reagents...
  39. ncbi Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae
    Mark A Sheff
    Bauer Center for Genomics Research, 7 Divinity Avenue, Harvard University, Cambridge, MA 02138, USA
    Yeast 21:661-70. 2004
    ..These tags significantly improve the detection limits for live-cell fluorescence imaging in yeast, and provide sufficient distinguishable fluorophores for four-colour imaging...
  40. ncbi A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish
    Koichi Kawakami
    Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Dev Cell 7:133-44. 2004
    ..We propose our gene trap approach should facilitate studies of vertebrate development and organogenesis...
  41. ncbi Localization of ASH1 mRNA particles in living yeast
    E Bertrand
    Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell 2:437-45. 1998
    ..Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization...
  42. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
    ..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
  43. pmc XRCC3 promotes homology-directed repair of DNA damage in mammalian cells
    A J Pierce
    Cell Biology Program, Memorial Sloan Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    Genes Dev 13:2633-8. 1999
    ..These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal...
  44. pmc Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification
    Morgan L Maeder
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Cell 31:294-301. 2008
    ..In summary, OPEN provides an "open-source" method for rapidly engineering highly active zinc-finger arrays, thereby enabling broader practice, development, and application of ZFN technology for biological research and gene therapy...
  45. pmc High-throughput in vivo analysis of gene expression in Caenorhabditis elegans
    Rebecca Hunt-Newbury
    Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS Biol 5:e237. 2007
    ..elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org)...
  46. pmc Circularly permuted green fluorescent proteins engineered to sense Ca2+
    T Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, Institute of Physical and Chemical Research (RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
    Proc Natl Acad Sci U S A 98:3197-202. 2001
    ..The Ca(2+)-dependent interaction between calmodulin and M13 in HeLa cells was monitored by the association of the two halves of GFP, neither of which was fluorescent by itself...
  47. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  48. ncbi Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
    L Timmons
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Gene 263:103-12. 2001
    ....
  49. ncbi CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance
    Jan Hendrik Niess
    Gastrointestinal Unit, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
    Science 307:254-8. 2005
    ..Thus, CX3CR1-dependent processes, which control host interactions of specialized DCs with commensal and pathogenic bacteria, may regulate immunological tolerance and inflammation...
  50. ncbi Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
    M A Cowley
    The Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA
    Nature 411:480-4. 2001
    ..On the basis of our results, we propose an integrated model of leptin action and neuronal architecture in the arcuate nucleus of the hypothalamus...
  51. ncbi Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues
    Konrad Hochedlinger
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 121:465-77. 2005
    ..These data show that certain adult progenitors remain competent to interpret key embryonic signals and support the notion that progenitor cells are a driving force in tumorigenesis...
  52. ncbi High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
    Chris J Janse
    Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
    Mol Biochem Parasitol 145:60-70. 2006
    ..The combination of enhanced transfection efficiency and a versatile rodent model provides a basis for the further development of novel tools for high throughput genome manipulation...
  53. pmc Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos
    Alexander Pfeifer
    The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:2140-5. 2002
    ..Ability to transfer foreign genes into human ES cells has potential relevance for the development of gene and cell-based therapies...
  54. ncbi Neural stem and progenitor cells in nestin-GFP transgenic mice
    John L Mignone
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Comp Neurol 469:311-24. 2004
    ....
  55. pmc The carnegie protein trap library: a versatile tool for Drosophila developmental studies
    Michael Buszczak
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 175:1505-31. 2007
    ....
  56. ncbi Direct isolation of satellite cells for skeletal muscle regeneration
    Didier Montarras
    CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France
    Science 309:2064-7. 2005
    ..Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity...
  57. ncbi Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators
    George T Hanson
    Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Biol Chem 279:13044-53. 2004
    ..T. Dooley, T. M. Dore, G. Hanson, W. C. Jackson, S. J. Remington, and R. Y. Tsien, submitted for publication), it is shown that the cytosol of HeLa cells is also unusually reducing but somewhat less so than the mitochondrial matrix...
  58. pmc Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
    B Grant
    Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
    Mol Biol Cell 10:4311-26. 1999
    ..We describe a new member of the low-density lipoprotein receptor superfamily, RME-2, identified in our screens for endocytosis defective mutants. We show that RME-2 is the C. elegans yolk receptor...
  59. ncbi Fluorescent proteins and their applications in imaging living cells and tissues
    Dmitriy M Chudakov
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    Physiol Rev 90:1103-63. 2010
    ..Here we focus on the structure, evolution, and function of GFP-like proteins and their numerous applications for in vivo imaging, with particular attention to recent techniques...
  60. pmc Visualization of the intracellular behavior of HIV in living cells
    David McDonald
    Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Cell Biol 159:441-52. 2002
    ..Based on these observations, we propose that HIV uses dynein and the microtubule network to facilitate the delivery of the viral genome to the nucleus of the cell during early postentry steps of the HIV life cycle...
  61. ncbi Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system
    Corinna Burger
    Department of Molecular Genetics and Microbiology, University of Florida, Gainesville 32610, USA
    Mol Ther 10:302-17. 2004
    ..Retrograde transport of rAAV1 and rAAV5 was also observed in particular CNS areas. These results suggest that vectors based on distinct AAV serotypes can be chosen for specific applications in the nervous system...
  62. pmc Electroporation and RNA interference in the rodent retina in vivo and in vitro
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16-22. 2004
    ..Plasmid DNA was also efficiently transfected into retinal explants in vitro by high-voltage pulses...
  63. pmc Use of the viral 2A peptide for bicistronic expression in transgenic mice
    Georgios Trichas
    Department of Physiology Anatomy and Genetics, University of Oxford, Oxford, UK
    BMC Biol 6:40. 2008
    ..They work in a wide variety of eukaryotic cells, but to date have not been demonstrated to function in transgenic mice in an inheritable manner...
  64. ncbi The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences
    M L Donnelly
    Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    J Gen Virol 82:1027-41. 2001
    ....
  65. ncbi Optical sectioning deep inside live embryos by selective plane illumination microscopy
    Jan Huisken
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Science 305:1007-9. 2004
    ..We also demonstrate that SPIM can be applied to visualize the embryogenesis of the relatively opaque Drosophila melanogaster in vivo...
  66. ncbi Capturing and profiling adult hair follicle stem cells
    Rebecca J Morris
    Department of Dermatology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Nat Biotechnol 22:411-7. 2004
    ..Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair...
  67. ncbi A modular design for the clathrin- and actin-mediated endocytosis machinery
    Marko Kaksonen
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Cell 123:305-20. 2005
    ....
  68. pmc Movement of Bax from the cytosol to mitochondria during apoptosis
    K G Wolter
    Biochemistry Section, Surgical Neurology Branch, Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 139:1281-92. 1997
    ....
  69. ncbi High-level transduction and gene expression in hematopoietic repopulating cells using a human immunodeficiency [correction of imunodeficiency] virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoter
    Christophe Demaison
    Molecular Immunology Unit, Institute of Child Health, UCL, 30 Guilford Street, London, WC1N 1EH, UK
    Hum Gene Ther 13:803-13. 2002
    ....
  70. pmc A critical period for enhanced synaptic plasticity in newly generated neurons of the adult brain
    Shaoyu Ge
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Neuron 54:559-66. 2007
    ....
  71. ncbi Disruption of fusion results in mitochondrial heterogeneity and dysfunction
    Hsiuchen Chen
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 280:26185-92. 2005
    ..In contrast, fragmentation of mitochondria, without severe loss of fusion, did not result in such cellular defects. Our results showed that key cellular functions decline as mitochondrial fusion is progressively abrogated...
  72. pmc Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease
    Katharina Lahl
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
    J Exp Med 204:57-63. 2007
    ..Furthermore, DEREG mice will allow a more precise definition of the function of Foxp3+ T reg cells in immune reactions in vivo...
  73. ncbi Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages
    N Faust
    Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Blood 96:719-26. 2000
    ..Mice with an inactivation of both copies of the lys gene developed normally and were fertile. (Blood. 2000;96:719-726)..
  74. ncbi Engineering stability in gene networks by autoregulation
    A Becskei
    EMBL, Structures and Biocomputing, Heidelberg, Germany
    Nature 405:590-3. 2000
    ..Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback...
  75. ncbi Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum
    Mikio Hoshino
    Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Neuron 47:201-13. 2005
    ..Our results suggest that Ptf1a is involved in driving neural precursors to differentiate into GABAergic neurons in the cerebellum...
  76. ncbi Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period
    Bidisha Chattopadhyaya
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 24:9598-611. 2004
    ....
  77. pmc Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA promoter activity
    Natalia J Martinez
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genome Res 18:2005-15. 2008
    ..Finally, our data suggest that post-transcriptional mechanisms contribute to differential miRNA expression. The data and strains described here will provide a valuable guide and resource for the functional analysis of C. elegans miRNAs...
  78. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
    ..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
  79. pmc Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
    Hiromi Imamura
    Precursory Research for Embryonic Science, Japan Science and Technology Agency, 5 Sanbancho, Chiyoda ku, Tokyo 102 0075, Japan
    Proc Natl Acad Sci U S A 106:15651-6. 2009
    ..This was also confirmed by an experiment using oligomycin A, an inhibitor of F(o)F(1)-ATP synthase. In addition, it was demonstrated that HeLa cells change ATP-generating pathway in response to changes of nutrition in the environment...
  80. ncbi Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells
    Takaki Miyata
    Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, Saitama 351 0198, Japan
    Development 131:3133-45. 2004
    ..The asymmetric P/P division may therefore contribute to efficient neuron/progenitor segregation required for CP initiation through cell cycle-dependent and lineage-restricted expression of Ngn2...
  81. ncbi Effective targeted gene 'knockdown' in zebrafish
    A Nasevicius
    Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA
    Nat Genet 26:216-20. 2000
    ..Conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebrafish embryos...
  82. pmc Translational control and target recognition by Escherichia coli small RNAs in vivo
    Johannes H Urban
    Max Planck Institute for Infection Biology, RNA Biology Group, Chariteplatz 1, 10117 Berlin, Germany
    Nucleic Acids Res 35:1018-37. 2007
    ..We expect our GFP fusion approach to be applicable to sRNA targets of other bacteria, and also demonstrate that Vibrio RyhB sRNA represses a Vibrio sodB fusion when co-expressed in E.coli...
  83. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
    ..Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes...
  84. ncbi A synthetic oscillatory network of transcriptional regulators
    M B Elowitz
    Department of Molecular Biology and Physics, Princeton University, New Jersey 08544, USA
    Nature 403:335-8. 2000
    ..Such 'rational network design may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks...
  85. pmc Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish
    Jon M Davison
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Dev Biol 304:811-24. 2007
    ....
  86. pmc Processing bodies require RNA for assembly and contain nontranslating mRNAs
    Daniela Teixeira
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    RNA 11:371-82. 2005
    ..Fourth, multiple translation factors and ribosomal proteins are lacking from P-bodies. These results suggest additional biological roles of P-bodies in addition to being sites of mRNA degradation...
  87. ncbi Development and use of fluorescent protein markers in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:87-91. 2003
    ....
  88. pmc A novel eGFP-expressing immunodeficient mouse model to study tumor-host interactions
    Simone P Niclou
    CRP Sante, Norlux Neuro Oncology Laboratory, 84, Val Fleuri, L 1526 Luxembourg
    FASEB J 22:3120-8. 2008
    ..Thus, the model described should provide significant insight into how tumor cells communicate with their microenvironment...
  89. pmc Atg8 controls phagophore expansion during autophagosome formation
    Zhiping Xie
    Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:3290-8. 2008
    ..This model provides a foundation for future analyses of the functions and dynamics of known autophagy-related proteins and for screening new genes...
  90. pmc Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control
    Adam C Fisher
    School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 3:e2351. 2008
    ....
  91. ncbi daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans
    S T Henderson
    Institute for Behavioral Genetics, University of Colorado at Boulder, Campus Box 447, Boulder, CO 80309 0447, USA
    Curr Biol 11:1975-80. 2001
    ..We suggest that changes in the subcellular localization of DAF-16 by environmental cues allows for rapid reallocation of resources in response to a changing environment at all stages of life...
  92. ncbi Effective gene therapy with nonintegrating lentiviral vectors
    Rafael J Yáñez-Muñoz
    Molecular Immunology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Nat Med 12:348-53. 2006
    ..For therapeutic application to postmitotic tissues, this system substantially reduces the risk of insertional mutagenesis...
  93. pmc Identification of the nuclear localization and export signals of high risk HPV16 E7 oncoprotein
    Alixandra A Knapp
    Biology Department, Boston College, Higgins Hall, Room 578, 140 Commonwealth Avenue, Chestnut Hill, MA 02467, USA
    Virology 383:60-8. 2009
    ..The presence of both NLSs and an NES suggests that HPV16 E7 shuttles between the cytoplasm and nucleus which is consistent with E7 having functions in both of these cell compartments...
  94. ncbi A role for NBR1 in autophagosomal degradation of ubiquitinated substrates
    Vladimir Kirkin
    Institute of Biochemistry II, Goethe University, Frankfurt Main, Germany
    Mol Cell 33:505-16. 2009
    ..We propose that NBR1 and p62 act as receptors for selective autophagosomal degradation of ubiquitinated targets...
  95. pmc Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway
    Eek Hoon Jho
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 22:1172-83. 2002
    ..Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal...
  96. ncbi APC and GSK-3beta are involved in mPar3 targeting to the nascent axon and establishment of neuronal polarity
    Song Hai Shi
    Departments of Physiology and Biochemistry, Howard Hughes Medical Institute, University of California at San Francisco, 1550 4th Street, San Francisco, CA 94143 0725, USA
    Curr Biol 14:2025-32. 2004
    ....
  97. pmc Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells
    Paul Gadue
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1496, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 103:16806-11. 2006
    ....
  98. ncbi Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus
    Qian Zhang
    Genelux Corporation, San Diego Science Center, San Diego, California 92109, USA
    Cancer Res 67:10038-46. 2007
    ..These findings suggest that immune activation may combine with viral oncolysis to induce tumor eradication in this model, providing a novel perspective for the design of oncolytic viral therapies for human cancers...
  99. ncbi Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior
    Henry J Klassen
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Invest Ophthalmol Vis Sci 45:4167-73. 2004
    ..To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration...
  100. pmc Novel AAV serotypes for improved ocular gene transfer
    Corinna Lebherz
    Gene Therapy Program, Division of Medical Genetics, Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA
    J Gene Med 10:375-82. 2008
    ..We show here that AAV7 and 8 enable superior long-term transduction of retinal and also anterior chamber structures...
  101. ncbi Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction
    Yoshinori Miyahara
    Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
    Nat Med 12:459-65. 2006
    ..Thus, transplantation of monolayered mesenchymal stem cells may be a new therapeutic strategy for cardiac tissue regeneration...

Research Grants66

  1. Assessing Protein Interactions in Cells by Fluorescence
    William Clark; Fiscal Year: 2003
    ..By fusing blue and green fluorescent proteins (BFP, GFP and/or YFP/CFP) to the receptor chains and expressing them in cells, these fluorophores could ..
  2. Structure-based Directed Evolution of Fast-Maturing GFPs
    Rebekka Wachter; Fiscal Year: 2007
    ..We propose to develop fast-maturing GFPs (green fluorescent proteins) by directed evolution, as guided by structural and mechanistic knowledge...
  3. The relative roled of sex and genes during development of systemic autoimmunity
    TRINE NORGAARD JORGENSEN; Fiscal Year: 2010
    ..To do so, we will target immune cells of a given sex with green fluorescent proteins prior to transfer into sex-matched or mismatched lethally irradiated recipients...
  4. A novel model for studying fetal skeletal muscle development
    Min Du; Fiscal Year: 2009
    ..pDsRed and pAcGFP vectors are mammalian expression vectors and express red and green fluorescent proteins respectively...
  5. Electrostatics and Dynamics in Proteins
    Steven G Boxer; Fiscal Year: 2013
    ..information on fields and their effects on function at the active sites of seveal enzymes and green fluorescent proteins (GFPs)...
  6. Small Animal Tomography System for Green Fluorescent Protein Imaging
    Andreas H Hielscher; Fiscal Year: 2011
    ..this proposal is to develop image reconstruction schemes and instrumentation for tomographic imaging of green fluorescent proteins (GFP) in small animals...
  7. High Speed 10-Color Flow Cytometer
    DEBRA LYNN LASKIN; Fiscal Year: 2012
    ..Paired with the use of monoclonal antibodies, green fluorescent proteins, and other fluorescent indicator dyes, cell subsets have been identified and characterized that have led ..
  8. (PQD5) Development of in Vitro Vascularized Microtumors for Drug Screening
    Christopher C W Hughes; Fiscal Year: 2013
    ..In addition, lentiviral-mediated expression of red, yellow and green fluorescent proteins in the tumor cells, endothelial cells and stromal cells will allow for repeate, rapid and quantitative ..
  9. IONIC CONTROL OF CARDIAC MUSCLE CONTRACTION: EC COUPLING
    Donald Bers; Fiscal Year: 2004
    ..This will be examined using new fluorescence resonance energy transfer probes, based on modified green fluorescent proteins linked by calmodulin biding peptides. This will provide fundamental new data about this system. 3...
  10. 3 to15 Covalent Gold for High Resolution Electron Microscopy
    VISHWAS JOSHI; Fiscal Year: 2009
    ..Clonable expression tags will be used in which yellow and green fluorescent proteins (YFP and GFP respectively) are expressed in combination with hexahistidine tag (His-tag) and lac ..
  11. MYOFIBRILLOGENESIS IN LIVING CARDIAC MUSCLE CELLS
    Joseph Sanger; Fiscal Year: 2006
    ..The experimental approaches include the use of plasmids encoding members of the group of Green Fluorescent Proteins ligated to sarcomeric proteins and mutated domains for transfections of cardiomyocytes...
  12. Small Animal Tomography System for Green Fluorescent Protein Imaging
    ANDREAS HIELSCHER; Fiscal Year: 2007
    ..this proposal is to develop image reconstruction schemes and instrumentation for tomographic imaging of green fluorescent proteins (GFP) in small animals...
  13. Live Cell Imaging with Fluorescent Polymer Bioconjugates
    JOONGHO MOON; Fiscal Year: 2005
    ..commonly used fluorophores in cell imaging, cell tracking, and in vivo imaging are organic molecules or green fluorescent proteins (GFPs). They have proven valuable in optical imaging and been applied to the study of single molecules...
  14. MYOFIBRILLOGENESIS IN LIVING SKELETAL MUSCLE CELLS
    Joseph Sanger; Fiscal Year: 2004
    ..single living skeletal muscle cells transfected with probes expressing sarcomeric proteins linked to Green Fluorescent Proteins. Our second approach is focused on a molecular analysis of proteins interacting with the Z-bodies and Z-..
  15. GASTRIC NEUROPEPTIDASES/PEPTIDE RECEPTOR INTERACTIONS
    Catia Sternini; Fiscal Year: 2002
    ..sites and pH at which these occur; and d) identifying interactions between receptors tagged with green fluorescent proteins, Cy3 labeled GRP, and other molecules that are colocalized at different stages of endocytosis and ..
  16. Stereology & Live Cell Imaging Workstation
    Anda Cornea; Fiscal Year: 2004
    ..For other projects, use of green fluorescent proteins and their mutants has created opportunities for identifying the intracellular location, translocation ..
  17. Mosiac Analysis of Synaptic Development in Mice
    Guoping Feng; Fiscal Year: 2006
    ..generated many lines of transgenic mice in which a small number of individual neurons are labeled with green fluorescent proteins (GFP) in a Golgi-like fashion, allowing novel analyses of processes such as axonal guidance, dendritic ..
  18. YEAST BASED NUCLEAR REPORTER LIFETIME FRET ASSAY SYSTEM
    Salvador Fernandez; Fiscal Year: 2000
    ..The approach is based on the use of green fluorescent proteins as an intracellular market, and fluorescence lifetime sensing to quantify reporter gene expression...
  19. ACQUISITION OF A HIGH SPEED 7 COLOR FACS INSTRUMENT
    Troy Randall; Fiscal Year: 2001
    ..to examine UV dependent parameters, such as low level Ca2+ flux, Hoescht staining of DNA, and some types of green fluorescent proteins. Therefore, we feel that the MoFlo is the instrument of choice for the Institute's investigators.
  20. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  22. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  23. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  24. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  25. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  26. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  27. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  28. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  29. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  30. NEURAL REGULATION OF VASOPRESSIN RELEASE
    J Thomas Cunningham; Fiscal Year: 2010
    ..Benefits: The results will provide new information regarding the control of vasopressin secretion and how these systems contribute to pathophysiology. ..
  31. CONTROL OF SODIUM INTAKE IN THE HINDLIMB UNWEIGHTED RAT
    J Cunningham; Fiscal Year: 2005
    ..Additionally, these data may contribute to understanding of health problems associated with an aging population and with cardiovascular disease such as congestive heart failure. ..
  32. LENTIVIRAL DELIVERY OF GDNF AND BCL-2 IN PD MODEL
    Marina Emborg; Fiscal Year: 2003
    ..These studies will serve as the preclinical foundation to determine whether this in vivo method to deliver these potent trophic factor and antiapoptotic genes will be suitable for testing in patients with PD. ..
  33. ACYLTRANSFERASE INHIBITORS AS ANTI RAS AGENTS
    Charles Smith; Fiscal Year: 2009
    ..abstract_text> ..
  34. Mathematical Models of Programmed CTL Proliferation
    Dominik Wodarz; Fiscal Year: 2009
    ..These are the role of effector molecules (such as perform and IFN-g) for CTL dynamics, and the role of CD4 T cell help. The modeling work will be complemented by experiments with VSV and LCMV infected mice. ..
  35. NPY DEPRESSION OF HIPPOCAMPAL EPILEPSY
    Anthony van den Pol; Fiscal Year: 2002
    ..NPY is potentially of great interest because its primary action in the normal hippocampus appears to be one of depressing hyperexcited neurons, without a substantial effect on normal neurotransmission. ..
  36. Hypocretin Neurons
    Anthony N van den Pol; Fiscal Year: 2010
    ..Understanding the role of the endogenous opioid in hypocretin cells should also facilitate the understanding and treatment of narcolepsy. ..
  37. Pitx2 Function in Developing Mouse Brain Neurons
    Donna M Martin; Fiscal Year: 2010
    ..These results will help guide future experiments aimed at directing neural stem cell differentiation toward specific neuronal cell subtypes. ..
  38. GENISTEIN AS MODULATOR OF ANTIOXIDANT PROTEIN EXPRESSION
    Shiu Ming Kuo; Fiscal Year: 2002
    ..These studies may also lead to the identification of a previous unknown pathway for Gn to regulate mammalian genes. ..
  39. Cytomegalovirus in the Brain
    Anthony N van den Pol; Fiscal Year: 2010
    ..These experiments will help us understand the mechanisms associated with CMV-induced neurological dysfunction, and how to combat the virus within the brain with minimal complications. ..
  40. Elucidate C. elegans Oxygen Sensing with Microfluidics
    Hang Lu; Fiscal Year: 2006
    ..The general techniques developed in this study should impact other C. elegans researches of sensing and behaviors such as odor discrimination and thermo-sensation. ..
  41. Gene drive potential of transposable elements in Anopheles gambiae.
    DAVID A O apos BROCHTA; Fiscal Year: 2010
    ..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
  42. Function and regulation of T-box factors in C. elegans
    Peter G Okkema; Fiscal Year: 2010
    ..elegans, and the results obtained will have a direct bearing on our understanding of these factors in all organisms. ..
  43. Regulation of SMAD4 and BMPR1A Expression in Juvenile Polyposis
    James R Howe; Fiscal Year: 2010
    ..abstract_text> ..
  44. STRUCTURE AND FUNCTION OF THE LH AND FSH RECEPTORS
    Deborah Segaloff; Fiscal Year: 2002
    ..Long term goals of research in this area include the ability to better understand, diagnose, and correct reproductive disorders. ..
  45. Conference on Photodynamic Therapy
    David Kessel; Fiscal Year: 2002
    ..The most recent PDT meeting, sponsored by the International Photodynamic Assn, has more than 600 participants. ..
  46. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 2001
    ....
  47. Imageable Mouse Models of Pancreatic Cancer
    Michael Bouvet; Fiscal Year: 2008
    ..Using such dual-colored imaging techniques, a better characterization of the tumor-host interactions in pancreatic cancer can be made. ..
  48. Gonadotropin Receptor Dimerization/Oligomerization
    Deborah Segaloff; Fiscal Year: 2008
    ..abstract_text> ..
  49. Function of a Neuropeptide Gene Family in C. elegans
    Christine Li; Fiscal Year: 2006
    ..In addition, because C. elegans is the current genetic model system for parasitic nematodes, elucidating the functions of neuropeptides and their signaling pathways may identify new antihelmintic drug targets. ..
  50. Proteomic charaterization of insulin signaling targets
    JONATHAN BOGAN; Fiscal Year: 2006
    ..Additionally, this work may have broader implications for insulin signaling and for protein targeting. [unreadable] [unreadable]..
  51. Redox control of Nrf2/ARE by thioredoxin and GSH
    Jason Hansen; Fiscal Year: 2005
    ..The roles of GSH and cytosolic and nuclear TRX during NRF2 activation and binding will provide an essential foundation for future developmental toxicology. ..
  52. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  53. Novel & Selective Small Molecular Inhibitors of Human Peptide Deformylase
    Hakim Djaballah; Fiscal Year: 2006
    ..Indeed, the likelihood that a compound interferes with both assays, and therefore behaves as a false positive in both assays, is vastly reduced compared to a strategy solely based on one technique. ..
  54. Genetic Reg. of Hematopoietic Stem Cell Mobilization
    Hartmut Geiger; Fiscal Year: 2007
    ..g. to muscle tissue, or to other hematopoietic niches. ..
  55. DRUG DISCOVERY CORE FACILITY
    Charles Smith; Fiscal Year: 2005
    ..Establishment of this DDCF will provide an important bridge between basic and applied research, and so will enhance the research environment and advance cancer research. ..
  56. Cleveland Cell Biology Symposium: Regulation
    ALAN TARTAKOFF; Fiscal Year: 2004
    ..The results should be an intense synthesis of knowledge which will be stimulating for both students and established faculty. ..
  57. Role of Pitx2 in the Mammalian Central Nervous System
    Donna Martin; Fiscal Year: 2005
    ..5-14.5) mouse diencephalon, 2) Develop and analyze mice with Pitx2 loss of function, and 3) Develop and analyze transgenic mice with CNS-specific Pitx2 gain of function. ..
  58. PHARYNGEAL MYOGENESIS AND ORGANOGENESIS IN C ELEGANS
    PETER OKKEMA; Fiscal Year: 2004
    ..Finally we are specifically examining role the PEB-l plays during pharyngeal development by isolating and characterizing apeb-1 mutant. ..
  59. IGF-I and its binding proteins in vascular smooth muscle cells
    Cunming Duan; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  60. Dissecting neural circuits by microfluidic-enabled rapid microscopy & ablation
    Hang Lu; Fiscal Year: 2008
    ..In addition, besides the contribution to C. elegans sensory biology, the technologies are widely applicable to areas such as developmental biology, and to other organisms. [unreadable] [unreadable]..
  61. Forward and Reverse Genetics in Mosquitoes Using an RNA Virus
    ZACH ADELMAN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  62. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2002
    ..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
  63. 2008 Viral Vectors for Gene Therapy, The Science of Gordon Research Conference
    Paul Robbins; Fiscal Year: 2008
    ..End of Abstract) [unreadable] [unreadable] [unreadable]..
  64. Inhibition of NF-KB to Facilitate Islet Transplantation
    Paul Robbins; Fiscal Year: 2003
    ..The successful completion of the proposed studies should determine if NF-kB inhibition in islets by peptide and gene mediated transduction is able to improve islet viability prior to and post-transplantation. ..
  65. Assemby and Recombination of dsRNA Bacteriophage phi8
    Leonard Mindich; Fiscal Year: 2004
    ..Since reverse genetic techniques and in vitro genomic packaging are not available for these viruses, the cystoviridae (dsRNA bacteriophages) offer a valuable model for system for the study of the eukaryotic systems. ..