luminescent proteins

Summary

Summary: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.

Top Publications

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
  2. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
  3. ncbi Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
  4. ncbi Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP
    G Feng
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 28:41-51. 2000
  5. ncbi Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
    Nathan C Shaner
    Nat Biotechnol 22:1567-72. 2004
  6. ncbi A guide to choosing fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Nat Methods 2:905-9. 2005
  7. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
  8. ncbi The green fluorescent protein
    R Y Tsien
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647, USA
    Annu Rev Biochem 67:509-44. 1998
  9. ncbi Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
    Jean Livet
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 450:56-62. 2007
  10. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998

Research Grants

  1. Engineering Molecular Sensors for Stem Cell Function
    David Schaffer; Fiscal Year: 2005
  2. Luminescent RNAs
    Andrej Luptak; Fiscal Year: 2013
  3. Near-IR Bioluminescence Imaging
    Stephen Miller; Fiscal Year: 2009
  4. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
  5. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
  6. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
  7. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
  8. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
  9. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
  10. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007

Detail Information

Publications299 found, 100 shown here

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  2. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
    ..When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules...
  3. ncbi Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
    ....
  4. ncbi Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP
    G Feng
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 28:41-51. 2000
    ..In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal...
  5. ncbi Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
    Nathan C Shaner
    Nat Biotechnol 22:1567-72. 2004
    ..Three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies...
  6. ncbi A guide to choosing fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Nat Methods 2:905-9. 2005
    ....
  7. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before...
  8. ncbi The green fluorescent protein
    R Y Tsien
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647, USA
    Annu Rev Biochem 67:509-44. 1998
    ..Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories...
  9. ncbi Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
    Jean Livet
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 450:56-62. 2007
    ..The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale...
  10. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
    ..Thus, these plasmids should further facilitate the rapid analysis of gene function in S. cerevisiae...
  11. ncbi 'Green mice' as a source of ubiquitous green cells
    M Okabe
    Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEBS Lett 407:313-9. 1997
    ..The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments...
  12. pmc Improving the photostability of bright monomeric orange and red fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nat Methods 5:545-51. 2008
    ..and TagRFP (a monomeric derivative of eqFP578 from Entacmaea quadricolor) that maintain most of the beneficial qualities of the original proteins and perform as reliably as Aequorea victoria GFP derivatives in fusion constructs...
  13. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
  14. pmc An expanded palette of genetically encoded Ca²⁺ indicators
    Yongxin Zhao
    Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
    Science 333:1888-91. 2011
    ..This palette of indicators paints the way to a colorful new era of Ca(2+) imaging...
  15. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  16. pmc Improving FRET dynamic range with bright green and red fluorescent proteins
    Amy J Lam
    Department of Bioengineering, Stanford University, Stanford, California, USA
    Nat Methods 9:1005-12. 2012
    ..These improvements enhance detection of transient biochemical events such as neuronal action-potential firing and RhoA activation in growth cones...
  17. pmc Bright and stable near-infrared fluorescent protein for in vivo imaging
    Grigory S Filonov
    Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA
    Nat Biotechnol 29:757-61. 2011
    ..Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra...
  18. ncbi A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants
    Brook K Nelson
    Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996 0840, USA
    Plant J 51:1126-36. 2007
    ..In addition, the Arabidopsis organelle marker lines can also be employed in plant cell biology teaching labs to demonstrate the distribution and dynamics of these organelles...
  19. ncbi FACS-optimized mutants of the green fluorescent protein (GFP)
    B P Cormack
    Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
    Gene 173:33-8. 1996
    ..coli, the folding of the mutant proteins is more efficient than folding of wt GFP. These two properties contribute to a greatly increased (100-fold) fluorescence intensity, making the mutants useful for a number of applications...
  20. pmc Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:10554-9. 2004
    ..Our study provides an important guide for the development and improvement of indicators using GFP-based FRET...
  21. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..Thus FRET between GFP mutants can monitor localized Ca2+ signals and protein heterodimerization in individual live cells...
  22. ncbi Green fluorescent protein as a marker for gene expression
    M Chalfie
    Department of Biological Sciences, Columbia University, New York, NY 10027
    Science 263:802-5. 1994
    ..Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms...
  23. ncbi Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
    Vsevolod V Belousov
    Laboratory of Genes for Regeneration, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya Street 16 10, Moscow 117997, Russia
    Nat Methods 3:281-6. 2006
    ..Moreover, sensitivity of the probe was sufficient to observe H(2)O(2) increase upon physiological stimulation. Using HyPer we detected temporal increase in H(2)O(2) in the cytoplasm of PC-12 cells stimulated with nerve growth factor...
  24. ncbi A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein
    J Nakai
    Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki, 444 8585, Japan
    Nat Biotechnol 19:137-41. 2001
    ..G-CaMP will be a useful tool for visualizing intracellular Ca2+ in living cells. Mutational analysis, together with previous structural information, suggests the residues that may alter the fluorescence of GFP...
  25. ncbi Localization of ASH1 mRNA particles in living yeast
    E Bertrand
    Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell 2:437-45. 1998
    ..Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization...
  26. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
    ..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
  27. pmc Circularly permuted green fluorescent proteins engineered to sense Ca2+
    T Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, Institute of Physical and Chemical Research (RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
    Proc Natl Acad Sci U S A 98:3197-202. 2001
    ..The Ca(2+)-dependent interaction between calmodulin and M13 in HeLa cells was monitored by the association of the two halves of GFP, neither of which was fluorescent by itself...
  28. pmc Far-red fluorescent tags for protein imaging in living tissues
    Dmitry Shcherbo
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Miklukho Maklaya 16 10, Moscow, Russia
    Biochem J 418:567-74. 2009
    ..Together, monomeric mKate2 and pseudo-monomeric tdKatushka2 represent the next generation of extra-bright far-red fluorescent probes offering novel possibilities for fluorescent imaging of proteins in living cells and animals...
  29. ncbi Fluorescent proteins and their applications in imaging living cells and tissues
    Dmitriy M Chudakov
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    Physiol Rev 90:1103-63. 2010
    ..Here we focus on the structure, evolution, and function of GFP-like proteins and their numerous applications for in vivo imaging, with particular attention to recent techniques...
  30. pmc A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules
    Laura A Banaszynski
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Cell 126:995-1004. 2006
    ..This general strategy for regulating protein stability should enable conditional perturbation of specific proteins with unprecedented control in a variety of experimental settings...
  31. ncbi Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications
    O Griesbeck
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla, California 92093-0647, USA
    J Biol Chem 276:29188-94. 2001
    ..Citrine is superior to all previous YFPs except when pH or halide sensitivity is desired and is particularly advantageous within genetically encoded fluorescent indicators of physiological signals...
  32. pmc Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
    Amy E Palmer
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 101:17404-9. 2004
    ....
  33. ncbi The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences
    M L Donnelly
    Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    J Gen Virol 82:1027-41. 2001
    ....
  34. ncbi Fluorescent proteins from nonbioluminescent Anthozoa species
    M V Matz
    Institute of Bioorganic Chemistry, Russian Acadmy of Science, 117871 Moscow, Russia
    Nat Biotechnol 17:969-73. 1999
    ..The usefulness of the new proteins for in vivo labeling was demonstrated by expressing them in mammalian cell culture and in mRNA microinjection assays in Xenopus embryos...
  35. pmc Imaging large-scale neural activity with cellular resolution in awake, mobile mice
    Daniel A Dombeck
    Department of Molecular Biology, Carl Icahn Labs, Princeton University, Princeton, NJ 08544, USA
    Neuron 56:43-57. 2007
    ..Behaviorally correlated calcium transients from large neuronal and astrocytic populations were routinely measured, with an estimated motion-induced false positive error rate of <5%...
  36. pmc In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2
    Benjamin R Arenkiel
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 54:205-18. 2007
    ....
  37. ncbi A synthetic oscillatory network of transcriptional regulators
    M B Elowitz
    Department of Molecular Biology and Physics, Princeton University, New Jersey 08544, USA
    Nature 403:335-8. 2000
    ..Such 'rational network design may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks...
  38. ncbi Improved green fluorescence
    R Heim
    Nature 373:663-4. 1995
  39. pmc Probing cellular protein complexes using single-molecule pull-down
    Ankur Jain
    Center for Biophysics and Computational Biology and Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Nature 473:484-8. 2011
    ..SiMPull should provide a rapid, sensitive and robust platform for analysing protein assemblies in biological pathways...
  40. ncbi G protein signaling events are activated at the leading edge of chemotactic cells
    C A Parent
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cell 95:81-91. 1998
    ....
  41. ncbi Counting cytokinesis proteins globally and locally in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 310:310-4. 2005
    ..Proteins concentrated up to 100 times in contractile rings and 7500 times in spindle pole bodies at certain times in the cell cycle. This approach can be used to measure the global and local concentrations of any fusion protein...
  42. pmc Role of polo kinase and Mid1p in determining the site of cell division in fission yeast
    J Bahler
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 143:1603-16. 1998
    ..Given its previously known functions in mitosis and the timing of cytokinesis, Plo1p is thus implicated as a key molecule in the spatial and temporal coordination of cytokinesis with mitosis...
  43. pmc Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome
    Xiaokun Shu
    Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Science 324:804-7. 2009
    ..Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering...
  44. pmc A genetically encoded fluorescent sensor of ERK activity
    Christopher D Harvey
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 105:19264-9. 2008
    ..EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues...
  45. ncbi Regulation of noise in the expression of a single gene
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Genet 31:69-73. 2002
    ..Our results thus provide the first direct experimental evidence of the biochemical origin of phenotypic noise, demonstrating that the level of phenotypic variation in an isogenic population can be regulated by genetic parameters...
  46. pmc Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
    Hiromi Imamura
    Precursory Research for Embryonic Science, Japan Science and Technology Agency, 5 Sanbancho, Chiyoda ku, Tokyo 102 0075, Japan
    Proc Natl Acad Sci U S A 106:15651-6. 2009
    ..This was also confirmed by an experiment using oligomycin A, an inhibitor of F(o)F(1)-ATP synthase. In addition, it was demonstrated that HeLa cells change ATP-generating pathway in response to changes of nutrition in the environment...
  47. pmc The threshold for polyglutamine-expansion protein aggregation and cellular toxicity is dynamic and influenced by aging in Caenorhabditis elegans
    James F Morley
    Department of Biochemistry, Molecular Biology, and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 99:10417-22. 2002
    ....
  48. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
    ..Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes...
  49. pmc A monomeric red fluorescent protein
    Robert E Campbell
    Department of Pharmacology University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 99:7877-82. 2002
    ....
  50. pmc Control of stochasticity in eukaryotic gene expression
    Jonathan M Raser
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California San Francisco UCSF, 600 16th Street, Room S472D, San Francisco, CA 94143 2240, USA
    Science 304:1811-4. 2004
    ..These mutations suggest that noise is an evolvable trait that can be optimized to balance fidelity and diversity in eukaryotic gene expression...
  51. ncbi Near-infrared fluorescent proteins
    Dmitry Shcherbo
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia
    Nat Methods 7:827-9. 2010
    ..To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability...
  52. ncbi Development and use of fluorescent protein markers in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:87-91. 2003
    ....
  53. pmc A bright and photostable photoconvertible fluorescent protein
    Sean A McKinney
    Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Methods 6:131-3. 2009
    ..Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent...
  54. ncbi Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system
    Kensuke Yamauchi
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 66:4208-14. 2006
    ..This imaging technology will enable further understanding of the critical steps of metastasis and provide visible targets for antimetastasis drug development...
  55. ncbi In vivo imaging of neuronal activity by targeted expression of a genetically encoded probe in the mouse
    Thomas Bozza
    The Rockefeller University, New York, NY 10021, USA
    Neuron 42:9-21. 2004
    ..Targeted expression of synapto-pHluorin in mouse will permit the analysis of previously inaccessible neuronal populations and chronic imaging from genetically identified neurons in vivo...
  56. ncbi Self- and actin-templated assembly of Mammalian septins
    Makoto Kinoshita
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 3:791-802. 2002
    ..We conclude that septins alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that septins help organize these bundles...
  57. pmc Conversion of red fluorescent protein into a bright blue probe
    Oksana M Subach
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Chem Biol 15:1116-24. 2008
    ....
  58. pmc Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy
    Kateryna S Morozova
    Biophys J 99:L13-5. 2010
    ..TagRFP657 is shown to be an efficient protein tag for the superresolution fluorescence imaging using a commercially available stimulated emission depletion microscope...
  59. pmc Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 174:391-402. 2006
    ..Their absence delays late steps in cytokinesis, including septum formation and cell separation...
  60. pmc Regulation of TMEM16A chloride channel properties by alternative splicing
    Loretta Ferrera
    Laboratory of Molecular Genetics, Istituto Giannina Gaslini, Largo G Gaslini 5, 16147 Genova
    J Biol Chem 284:33360-8. 2009
    ..Alternative splicing appears as an important mechanism to regulate the voltage and Ca(2+) dependence of the TMEM16A-dependent Cl(-) channels in a tissue-specific manner...
  61. pmc An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
    Ryoko Ando
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 99:12651-6. 2002
    ..Illumination of a focused UV pulse onto the soma of a Kaede-expressing neuron resulted in filling of all processes with red fluorescence, allowing visualization of contact sites between the red and green neurons of interest...
  62. ncbi Real-time imaging of cell-cell adherens junctions reveals that Drosophila mesoderm invagination begins with two phases of apical constriction of cells
    H Oda
    Tsukita Cell Axis Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Kyoto Research Park, Chudoji Minami machi, Shimogyo ku, Kyoto 600 8813, Japan
    J Cell Sci 114:493-501. 2001
    ..Movies available on-line: http://www.biologists.com/JCS/movies/jcs2073.html..
  63. ncbi Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators
    Colette T Dooley
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093 0647, USA
    J Biol Chem 279:22284-93. 2004
    ..g. epidermal growth factor and lysophosphatidic acid...
  64. ncbi Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos
    Elayne Provost
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genesis 45:625-9. 2007
    ..The successful application of this technology in zebrafish will be valuable for visually marking transgenic embryos and transgene-expressing cells, or in any situation where reliable expression of multiple transgenes is desired...
  65. pmc Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 175:377-82. 2006
    ....
  66. ncbi The fluorescent toolbox for assessing protein location and function
    Ben N G Giepmans
    National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Science 312:217-24. 2006
    ..Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted...
  67. ncbi Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex
    H Tabata
    Department of Molecular Neurobiology, Institute of DNA Medicine, Jikei University School of Medicine, Minato-ku, 105-8461, Tokyo, Japan
    Neuroscience 103:865-72. 2001
    ..Thus, this system is much easier and more efficient than virus-mediated gene transfer, and is useful for gain-of-function analysis of neural cell fate determination, migration, positioning and axon path-finding in mouse embryos...
  68. pmc Budding yeast chromosome structure and dynamics during mitosis
    C G Pearson
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 152:1255-66. 2001
    ..These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms...
  69. ncbi Dynamic binding of histone H1 to chromatin in living cells
    T Misteli
    National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 408:877-81. 2000
    ..These results support a model in which linker histones bind dynamically to chromatin in a stop-and-go mode...
  70. ncbi Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors
    Hui Wang Ai
    University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, Edmonton, Alberta T6G 2G2, Canada
    Nat Methods 5:401-3. 2008
    ..Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other...
  71. pmc Cerulean, Venus, and VenusY67C FRET reference standards
    Srinagesh V Koushik
    Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD, USA
    Biophys J 91:L99-L101. 2006
    ..Since the results from each method were in good agreement, the FRET efficiency value of each construct could be determined with high accuracy and precision, thereby justifying their use as standards...
  72. pmc Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy, and peroxisome degradation pathways
    J Kim
    Section of Microbiology, University of California, Davis, California 95616, USA
    Mol Biol Cell 10:1337-51. 1999
    ....
  73. pmc Wavelength mutations and posttranslational autoxidation of green fluorescent protein
    R Heim
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647
    Proc Natl Acad Sci U S A 91:12501-4. 1994
    ....
  74. ncbi In vivo imaging of light-emitting probes
    B W Rice
    Xenogen Corporation, 860 Atlantic Avenue, Alameda, CA 94501, USA
    J Biomed Opt 6:432-40. 2001
    ..Instrumentation for in vivo imaging developed at Xenogen is described and several examples of images of mice with bioluminescent cells are presented...
  75. ncbi Real-time kinetics of gene activity in individual bacteria
    Ido Golding
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 123:1025-36. 2005
    ..This greatly facilitates kinetic interpretations in terms of the integer-valued random processes that produce the fluctuations...
  76. pmc Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging
    Mark C Leake
    Clarendon Laboratory, Oxford Physics, University of Oxford, Parks Road, Oxford OX1 3PU, United Kingdom
    Proc Natl Acad Sci U S A 105:15376-81. 2008
    ..TatA complexes do not form in cells lacking TatBC, suggesting that TatBC controls the oligomeric state of TatA. Our data support the TatA polymerization model for the mechanism of Tat transport...
  77. ncbi Genetically encoded chloride indicator with improved sensitivity
    Olga Markova
    Institut de Neurobiologie de la Méditerranée INMED, INSERM U901, Parc Scientifique de Luminy, Marseille, France
    J Neurosci Methods 170:67-76. 2008
    ..This genetically encoded indicator offers a means for monitoring Cl and pH under different physiological conditions and high-throughput screening of pharmacological agents...
  78. pmc Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape
    H Sesaki
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 147:699-706. 1999
    ..Our results thus suggest that mitochondrial shape is normally controlled by a balance between division and fusion which requires Dnm1p and Fzo1p, respectively...
  79. pmc An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching
    Michele L Markwardt
    Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 6:e17896. 2011
    ..Thus, mCerulean3 is a bright, photostable cyan fluorescent protein which possesses several characteristics that are highly desirable for FRET experiments...
  80. pmc Superresolution imaging using single-molecule localization
    George Patterson
    Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Phys Chem 61:345-67. 2010
    ..Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales...
  81. ncbi Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius
    Gert Jan Kremers
    Section Molecular Cytology and Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM, Amsterdam, The Netherlands
    Biochemistry 45:6570-80. 2006
    ..Using the large lifetime difference between SCFP1 and SCFP3A enabled us to perform for the first time dual-lifetime imaging of spectrally identical fluorescent species in living cells...
  82. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    ..In conclusion, we have shown that Golgi-resident glycosylation enzymes recycle through the ER and that this novel pathway is the likely explanation for the nocodazole-induced Golgi scattering observed in interphase cells...
  83. ncbi Quantitative comparison of the sensitivity of detection of fluorescent and bioluminescent reporters in animal models
    Tamara Troy
    Xenogen Corporation, USA
    Mol Imaging 3:9-23. 2004
    ..The use of blue-shifted excitation filters is explored as a method to subtract out tissue autofluorescence and improve the sensitivity of fluorescent imaging...
  84. ncbi Green fluorescent protein-based halide indicators with improved chloride and iodide affinities
    L J Galietta
    Department of Medicine, Cardiovascular Research Institute, 1246 Health Sciences East Tower, University of California, San Francisco, CA 94143-0521, USA
    FEBS Lett 499:220-4. 2001
    ..The anion-sensitive mechanism of these indicators was established and their utility in cells was demonstrated using transfected cells expressing the cystic fibrosis transmembrane conductance regulator chloride channel...
  85. ncbi Macromolecular dynamics in living cell nuclei revealed by fluorescence redistribution after photobleaching
    A B Houtsmuller
    Department of Pathology, Josephine Nefkens Institute, Erasmus University Rotterdam, The Netherlands
    Histochem Cell Biol 115:13-21. 2001
    ..This review gives an overview of the current state of knowledge of nuclear structure and function. In particular, the different applications of FRAP technology to study the dynamics of GFP-tagged nuclear proteins will be summarised...
  86. pmc Diversity and evolution of coral fluorescent proteins
    Naila O Alieva
    Section of Integrative Biology, University of Texas at Austin, Austin, Texas, United States of America
    PLoS ONE 3:e2680. 2008
    ....
  87. ncbi Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins
    Hui Wang Ai
    University of Alberta, Department of Chemistry, Edmonton, Alberta, Canada T6G 2G2
    Biochemistry 46:5904-10. 2007
    ..These new variants should greatly facilitate multicolor fluorescent imaging by legitimizing blue fluorescing proteins as practical and robust members of the fluorescent protein "toolkit"...
  88. ncbi Double-labelled HIV-1 particles for study of virus-cell interaction
    Marko Lampe
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Virology 360:92-104. 2007
    ..We present here a detailed characterization of this novel tool together with exemplary live cell imaging studies, demonstrating its suitability for real-time analyses of HIV-cell interaction...
  89. pmc Green fluorescent protein functions as a reporter for protein localization in Escherichia coli
    B J Feilmeier
    Department of Microbiology, Iowa State University, Ames 50011, USA
    J Bacteriol 182:4068-76. 2000
    ..These results suggest that GFP could serve as a useful reporter for genetic analysis of bacterial protein export and of protein folding...
  90. ncbi The multiple uses of fluorescent proteins to visualize cancer in vivo
    Robert M Hoffman
    AntiCancer Inc and Department of Surgery, University of California, San Diego, 7917 Ostrow Street, San Diego, California 92111, USA
    Nat Rev Cancer 5:796-806. 2005
    ..Visualization of many aspects of cancer initiation and progression in vivo should be possible with fluorescent proteins...
  91. ncbi A change in the selective translocation of the Kinesin-1 motor domain marks the initial specification of the axon
    Catherine Jacobson
    Center for Research on Occupational and Environmental Toxicology, Oregon Health and Science University, Portland, Oregon 97239, USA
    Neuron 49:797-804. 2006
    ..Imaging YFP-tagged truncated Kinesin-1 provides the most precise definition to date of when neuronal polarity first emerges and allows visualization of the molecular differentiation of the axon in real time...
  92. ncbi Expression of exogenous genes in Trypanosoma cruzi: improving vectors and electroporation protocols
    Wanderson D DaRocha
    Departamento de Bioquimica e Imunologia, ICB, Universidade Federal de Minas Gerais, MG 31270 010, Belo Horizonte, Brazil
    Parasitol Res 92:113-20. 2004
    ..In contrast, the amastin 5' UTR plus Ig resulted in lower levels of reporter gene expression. We also constructed a vector containing an expression cassette designed to be targeted to the tubulin locus of the parasite...
  93. ncbi Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
    Carlos Lois
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:868-72. 2002
    ..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
  94. pmc Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor
    Janko Potzkei
    Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Juelich Research Center, Wilhelm Johnen Straße, D 52425 Juelich, Germany
    BMC Biol 10:28. 2012
    ..Therefore, real-time monitoring of cellular oxygen levels is basically a prerequisite for the analysis of hypoxia-induced processes in living cells and tissues...
  95. pmc Flavin mononucleotide (FMN)-based fluorescent protein (FbFP) as reporter for gene expression in the anaerobe Bacteroides fragilis
    Leandro A Lobo
    Instituto de Microbiologia Paulo de Gois, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    FEMS Microbiol Lett 317:67-74. 2011
    ..This suggests that ahpC and dps are induced following internalization by macrophages. Thus, we show that BS2 is a suitable tool for the detection of gene expression in obligate anaerobic bacteria in in vivo studies...
  96. pmc Enhanced beetle luciferase for high-resolution bioluminescence imaging
    Yoshihiro Nakajima
    National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan
    PLoS ONE 5:e10011. 2010
    ..These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc...
  97. ncbi Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging
    Chaincy Kuo
    Caliper Life Sciences, Xenogen Corporation, 2061 Challenger Drive, Alameda, California 94501, USA
    J Biomed Opt 12:024007. 2007
    ..Finally, reconstructions are demonstrated in a PC3M-luc (prostate tumor line) metastatic tumor model in nude mice...
  98. pmc Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
    Hari Shroff
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 104:20308-13. 2007
    ..The simplicity, minimal invasiveness, resolution, and speed of the technique all suggest its potential to directly visualize molecular interactions within cellular structures at the nanometer scale...
  99. pmc Inhibition of 2A-mediated 'cleavage' of certain artificial polyproteins bearing N-terminal signal sequences
    Pablo de Felipe
    Centre for Biomolecular Sciences, North Haugh, University of St Andrews, St Andrews, Scotland, UK
    Biotechnol J 5:213-23. 2010
    ..Solutions to this problem include the use of longer 2As (with a favourable upstream context) or modifying the order of proteins comprising polyproteins...
  100. ncbi Plastid tubules of higher plants are tissue-specific and developmentally regulated
    R H Köhler
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca NY 14853, USA
    J Cell Sci 113:81-9. 2000
    ..Photobleaching experiments indicated that GFP can flow through stromules and that the technique can be used to distinguish interconnected plastids from independent plastids...
  101. ncbi High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors
    Yue Ma
    National Primate Research Center, School of Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Stem Cells 21:111-7. 2003
    ..These results also have important implications for the possible future use of gene-modified human ES cells in transplantation and tissue regeneration applications...

Research Grants73

  1. Engineering Molecular Sensors for Stem Cell Function
    David Schaffer; Fiscal Year: 2005
    ..In particular, imaging genetically encoded sensors based on fluorescent and luminescent proteins, which can be permanently introduced into cells and organisms, can yield detailed information on numerous ..
  2. Luminescent RNAs
    Andrej Luptak; Fiscal Year: 2013
    ..Fluorescent and luminescent proteins are prime examples of tools that have had an enormous impact on biology...
  3. Near-IR Bioluminescence Imaging
    Stephen Miller; Fiscal Year: 2009
    ..Despite considerable effort spent isolating and mutagenizing luminescent proteins, there is no luciferase that maximally emits light >650 nm...
  4. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  5. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  6. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  7. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  8. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  9. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  10. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  11. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  12. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  13. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  14. MOLECULAR BIOLOGY OF MUTANT 5-HT2C SEROTONIN RECEPTORS
    Katharine Herrick Davis; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  15. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2008
    ..abstract_text> ..
  16. DNA helicase and primase inhibitors for biodefense
    Donald Moir; Fiscal Year: 2006
    ..Finally, compounds will be tested for lack of toxicity to mammalian cells in culture, and lack of activity against eukaryotic polymerase alpha and helicase, resulting in a collection of validated hits. ..
  17. Type III Secretion Inhibitors for Anti-Infective Therapy
    Donald Moir; Fiscal Year: 2007
    ..aeruginosa TTSS; (3) Screen a diverse compound library to identify and validate TTSS inhibitors; and (4) Prioritize validated screening hits for in vitro potency, mechanism, spectrum, and selectivity. [unreadable] [unreadable]..
  18. Rhabdovirus phosphoproteins: RNA silencing and complex formation
    Michael Goodin; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  19. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2006
    ..Such new techniques should help explore the biochemical mechanisms of long-term depression and a newly discovered form of long-term potentiation of synapses between parallel fibers and Purkinje neurons in young adult cerebellar slices. ..
  20. NEURONAL MIGRATION IN THE DEVELOPING BRAIN
    Huaiyu Hu; Fiscal Year: 2003
    ....
  21. Discovery of B. pseudomallei Therapeutics for Biodefense
    Donald Moir; Fiscal Year: 2008
    ..pseudomallei targets. In Phase III, a potent, safe, orally active B. pseudomallei inhibitor will be advanced into IND enabling toxicology and safety pharmacology studies and file an IND. [unreadable] [unreadable] [unreadable]..
  22. Imageable Mouse Models of Pancreatic Cancer
    Michael Bouvet; Fiscal Year: 2008
    ..Using such dual-colored imaging techniques, a better characterization of the tumor-host interactions in pancreatic cancer can be made. ..
  23. Role of Na, K-ATPase beta isoforms in pump sorting and epithelial integrity
    Olga Vagin; Fiscal Year: 2010
    ....
  24. GENETICALLY TARGETABLE LABELS FOR LIGHT AND EM
    Roger Tsien; Fiscal Year: 2007
    ..These activities benefit from the combination of this applicant teams expertise in chemistry, imaging instrument development, cell and molecular biology, physics and biophysics. ..
  25. Effects of intracellular angiotensin II
    Julia Cook; Fiscal Year: 2009
    ..abstract_text> ..
  26. Molecular Imaging of Cardiac Cell Transplantation
    Joseph Wu; Fiscal Year: 2008
    ..The goal is to use molecular imaging to guide stem cell therapy, which should translate into transplant protocols that are more reproducible, quantifiable, and beneficial. ..
  27. Nanostructuring and Molecular Imaging of Engineered Cardiovascular Tissues
    Joseph Wu; Fiscal Year: 2008
    ..With a multi-disciplinary team approach, we are confident that these combined efforts will accelerate the translation of nanotechnology-based tissue engineering to the clinical arena in the future. ..
  28. Molecular Studies of Brain Malformations
    Huaiyu Hu; Fiscal Year: 2007
    ....
  29. Sensing Biowarfare Agents by Surface Enhanced Raman
    Donald Moir; Fiscal Year: 2005
    ....
  30. Concurrent Immune Stimulation and Inhibition of Angiogenesis for Glioma Therapy
    JOHN OHLFEST; Fiscal Year: 2008
    ..The project has high impact potential because it may identify a new treatment approach for glioblastoma patients. [unreadable] [unreadable] [unreadable]..
  31. The Role of Triggering Receptor Expressed on Myeloid Cells-2 (Trem2) on Microglia
    Christine Hsieh; Fiscal Year: 2008
    ..The significance of understanding the role of TREM2 may be applied to multiple neurological and immunological diseases. [unreadable] [unreadable]..
  32. pH Regulation of Fat Storage in the Nematode Intestine
    Keith Nehrke; Fiscal Year: 2007
    ..The strength of this proposal is that it will use a powerful model system and novel reagents for monitoring pH in living organisms to study fat biology from a unique perspective: that of acid-base homeostasis. ..
  33. Coordination of signaling during phagocytosis
    Joel A Swanson; Fiscal Year: 2010
    ..Because these studies will measure signal amplitudes as a function of particle size, they should identify size thresholds for FcR signaling and determine the relative contribution of 3'Pls to those thresholds. ..
  34. Subsequent effects of manipulating embryonic neuronal activity
    Elwood Albert Linney; Fiscal Year: 2010
    ..The technologies used will include behavioral analyses, the creation and use of a number of fluorescent transgenic reporter lines to fish, microarray analysis, and HPLC analysis of neurotransmitter levels. ..
  35. Vesicle targeting in Plasmodium falciparum
    Julian Rayner; Fiscal Year: 2008
    ..By focusing on elements of P. falciparum secretion that are unique to this organism, we aim to identify targets for the future development of novel prevention and control measures. [unreadable] [unreadable] [unreadable]..
  36. Use of Gene Therapy: A Tool to Study Reproductive Function
    Ursula B Kaiser; Fiscal Year: 2010
    ..abstract_text> ..
  37. Selfish gene and genetic control of vector-borne diseases
    ZACH ADELMAN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  38. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David W Piston; Fiscal Year: 2010
    ..Second, understanding the cell-to-cell communication pathways may lead to new drugs that enhance insulin secretion for the treatment of Type II diabetes. ..
  39. Sentinel cells that report neural and neurovascular signaling
    David Kleinfeld; Fiscal Year: 2008
    ..The assay has wide ranging applications, including, but not limited to research on abused substances. [unreadable] [unreadable] [unreadable]..
  40. REPRODUCTIVE BIOLOGY OF GONADOTROPIN REGULATION
    URSULA KAISER; Fiscal Year: 2005
    ..abstract_text> ..
  41. Genetic Dissection of Brain Reward Circuits
    LARRY ZWEIFEL; Fiscal Year: 2008
    ..abstract_text> ..
  42. Gonadotropin Receptor Dimerization/Oligomerization
    Deborah Segaloff; Fiscal Year: 2008
    ..abstract_text> ..
  43. CENTER FOR ENVIRONMENTAL GENOMICS
    Elwood Linney; Fiscal Year: 2006
    ..In aggregates the coupled scientific findings from the proposed Program will substantially enhance our understanding of environmental toxicology and genomics. ..
  44. Analysis of sperm transfer behavior in C elegans
    Gary Schindelman; Fiscal Year: 2004
    ..These questions will be investigated by means of surgical and genetic methods. ..
  45. CARDIAC MYOSIN TRANSGENESIS:MOLECULAR DESIGN/PERFORMANCE
    David Warshaw; Fiscal Year: 2004
    ..These studies should provide important information about myosin's molecular structure and function and how alterations to myosin's molecular performance is mechanically expressed in the ventricular power output. ..
  46. Pregnancy Hormones and Membrane Estrogen Receptors
    Han Htun; Fiscal Year: 2003
    ..The research will open a new avenue of investigation in which the genome integrates the actions of both membrane and intracellular estrogen receptors. ..
  47. 2003 Gordon Research Conference - Biology of Aging
    John Tower; Fiscal Year: 2003
    ..Significant funding is also provided by the Gordon Research Conference Organization, and additional funds are being solicited from private foundations and industry. ..
  48. MOLECULAR IMAGING OF ADVANCED BLADDER CANCER
    JOHN FRANGIONI; Fiscal Year: 2002
    ..We believe that bladder cancer-specific, low-molecular weight ligands will someday be clinically useful reagents for improved detection and guided therapy of transitional cell carcinoma of the bladder. ..
  49. Spatiotemporal Analysis of Neoplasia in Animal Models
    Christopher Contag; Fiscal Year: 2006
    ..These goals will be met by generating a shared imaging research resource at Stanford University with the ability of spatiotemporal analyses of both structure and function in neoplastic disease models. ..
  50. Role of Fibroblast-Derived MT1-MMP in Oral Cancer
    Eben Rosenthal; Fiscal Year: 2006
    ..abstract_text> ..
  51. HIGH RESOLUTION 4-DIMENSIONAL FLUORESCENCE MICROSCOPY
    Walter Carrington; Fiscal Year: 2002
    ..Sophisticated image restoration, volume visualization and data analysis software will be provided at the microscope for this purpose. ..
  52. VANDERBILT IN VIVO IMAGING CENTER
    David Piston; Fiscal Year: 2002
    ..Collaborations between projects and cores promise to validate current hypotheses about tumor vascularization, and to rapidly apply these finding to clinical cancer diagnosis and therapy. ..
  53. MOLECULAR SORTING DURING INTRACELLULAR TRANSPORT
    Ira Mellman; Fiscal Year: 2006
    ..5) To identify and characterize novel molecules that function during polarized sorting. Cell biological approaches will be applied together with mutant screens and expression analysis in Drosophila. ..
  54. VISIBLE ANIMAL MODELS OF NEOPLASTIC DISEASE
    Christopher Contag; Fiscal Year: 2002
    ..1 R33 CA88303-01 -3- Christopher Contag, Ph.D. ..
  55. Olympus FV1000 Laser Scanning Confocal Microscope
    William Bement; Fiscal Year: 2007
    ..This information, in turn, will help guide future investigations of a number of normal and pathological human processes such as wound repair, nerve regeneration, cancer, and birth defects. [unreadable] [unreadable] [unreadable]..
  56. VISUALIZING TUMORS AND MECHANISMS OF THERAPY IN VIVO
    Robert Negrin; Fiscal Year: 2007
    ..These insights will be utilized to develop strategies translatable to the clinic to improve outcomes for patients with a broad range of malignant conditions. ..
  57. Gene Therapy for Correction of PiZ Mutation
    Sihong Song; Fiscal Year: 2006
    ..Liver pathology will be examined. We anticipate achieving the therapeutic correction in PiZ transgenic mouse model. The success of this project may lead to a novel gene therapy for clinical treatment of human AAT deficiency. ..
  58. Na+/H+ exchange in the nematode intestine
    Keith Nehrke; Fiscal Year: 2004
    ..This multidisciplinary approach will help to define the biological role of individual Na+/H+ exchangers in the nematode intestine. ..
  59. ER Chaperone Availability in Cells During Homeostasis and Misfolded Protein Stres
    Erik L Snapp; Fiscal Year: 2010
    ..We are studying, at the cellular level, the mechanisms that maintain and regulate the secretory protein folding environment. ..
  60. ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERY
    Natalya Y Rapoport; Fiscal Year: 2010
    ....
  61. High density mutagenesis of mtDNA
    Mikhail Alexeyev; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  62. Cell Biology of Human Thiamine Transporters
    VEEDAMALI SUBRAMANIAN; Fiscal Year: 2005
    ....
  63. Structure-based Directed Evolution of Fast-Maturing GFPs
    Rebekka Wachter; Fiscal Year: 2007
    ..In more general terms, a fast-maturing GFP would aid in the development of therapies for a very large number of disease states including neurodegenerative and cancerous disorders. [unreadable] [unreadable] [unreadable]..
  64. Polycystic Liver Disease and ER Quality Control
    Erik Snapp; Fiscal Year: 2007
    ..These studies will provide some of the first insights into the cell biology of PCLD and will identify substrates that require the activities of Sec63 and glucosidase IIb. [unreadable] [unreadable] [unreadable]..
  65. Mesenchymal Stem Cells for Fetal Gene Delivery
    Alice Tarantal; Fiscal Year: 2006
    ..abstract_text> ..
  66. In vivo imaging of corneal inflammation
    Ellen Lee; Fiscal Year: 2008
    ..These studies will be an important contribution to the understanding of the corneal inflammatory process, which may lead to the development of novet strategies for its prevention and/or control. ..
  67. Noncoding RNA based platforms for in vivo biosensors
    KEVIN HOFF; Fiscal Year: 2008
    ..These studies are predicted to establish a biosensor platform with rapid detection kinetics that is easily evolvable, modular, and capable of allosteric control of mRNA expression. [unreadable] [unreadable] [unreadable]..
  68. ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERY
    Natalya Rapoport; Fiscal Year: 2001
    ....
  69. Center for Pediatric Stem/Progenitor Cell Translational Research
    Alice Tarantal; Fiscal Year: 2008
    ..If imaging techniques can be developed that allows one to monitor the cells over time, this would provide a very powerful tool to determine the fate of the cells after injection into humans. ..
  70. Center for Fetal Gene Transfer for HLB Diseases
    Alice Tarantal; Fiscal Year: 2005
    ..abstract_text> ..
  71. Identifying targets for diagnosing oral candidiasis
    David Schofield; Fiscal Year: 2005
    ..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..
  72. The role of MOZ in normal and leukemic hematopoiesis
    CYNTHIA SNYDER; Fiscal Year: 2007
    ..The long term goal of this project is to understand how MOZ contributes to commitment and terminal differentiation during hematopoiesis. ..