wheat germ agglutinins

Summary

Summary: Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.

Top Publications

  1. ncbi Wheat germ agglutinin-binding glycoproteins are decreased in Alzheimer's disease cerebrospinal fluid
    L R Fodero
    Department of Pathology, University of Melbourne, Victoria, Australia
    J Neurochem 79:1022-6. 2001
  2. ncbi Adsorptive endocytosis mediates the passage of HIV-1 across the blood-brain barrier: evidence for a post-internalization coreceptor
    W A Banks
    Veterans Affairs Medical Center and Tulane Univeristy School of Medicine, New Orleans, LA 70146, USA
    J Cell Sci 111:533-40. 1998
  3. pmc Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores
    D R Finlay
    J Cell Biol 104:189-200. 1987
  4. ncbi A genetic approach to visualization of multisynaptic neural pathways using plant lectin transgene
    Y Yoshihara
    Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, Saitama, Japan
    Neuron 22:33-41. 1999
  5. pmc Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes
    Li A Kung
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06620 8103, USA
    Mol Syst Biol 5:308. 2009
  6. ncbi Binding, internalization, and membrane incorporation of human immunodeficiency virus-1 at the blood-brain barrier is differentially regulated
    W A Banks
    GRECC, Veterans Affairs Medical Center St Louis and St Louis University School of Medicine, Division of Geriatrics, Department of Internal Medicine, St Louis, MO 63106, USA
    Neuroscience 128:143-53. 2004
  7. ncbi Genetic tracing shows segregation of taste neuronal circuitries for bitter and sweet
    Makoto Sugita
    Department of Physiology and Oral Physiology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1 2 3, Minami Ku, Hiroshima 734 8553, Japan
    Science 309:781-5. 2005
  8. ncbi Evidence that the species barrier of human immunodeficiency virus-1 does not extend to uptake by the blood--brain barrier: comparison of mouse and human brain microvessels
    William A Banks
    GRECC, Veterans Affairs Medical Center St Louis and Saint Louis University School of Medicine, Division of Geriatrics, Department of Internal Medicine, St Louis, MO 63106, USA
    Life Sci 77:2361-8. 2005
  9. ncbi Tracking mouse visual pathways with WGA transgene
    Yoko Hanno
    Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Eur J Neurosci 18:2910-4. 2003
  10. ncbi Effects of lectins on CCK-8-stimulated enzyme secretion and differentiation of the rat pancreatic cell line AR42J
    U Mikkat
    Department of Pathology, Faculty of Medicine, University of Rostock, Strempelstrasse 14, D-18055 Rostock, Germany
    Pancreas 23:368-74. 2001

Research Grants

  1. MEMBRANE ANTIGENS OF RAT PROSTATE ADENOCARCINOMA
    ALICE CLAFLIN; Fiscal Year: 1980
  2. Opiate Addiction and HIV-1 Induced Release of Cytokines
    William Banks; Fiscal Year: 2006
  3. Prion Transport Across the Blood-Brain Barrier
    William Banks; Fiscal Year: 2009
  4. Mechanisms of HIV Transport Across the BBB
    William Banks; Fiscal Year: 2004
  5. PTS1 Regulation of Brain Met Enk Levels in Alcoholism
    William Banks; Fiscal Year: 2004
  6. Determining Therapeutic Efficacy of AGE in AD
    Neelima Chauhan; Fiscal Year: 2005
  7. Reduction of Amyloid Burden by Antisense APP
    Neelima Chauhan; Fiscal Year: 2005
  8. Pathobiology of HMG-CoA reductase inhibitors in diabetes
    Yashpal Kanwar; Fiscal Year: 2006
  9. Dendritic cell function and ethanol
    ANNETTE SCHLUETER; Fiscal Year: 2007
  10. GLOMERULAR CAPILLARY WALL--NORMAL AND PATHOLOGIC
    Yashpal Kanwar; Fiscal Year: 2008

Detail Information

Publications186 found, 100 shown here

  1. ncbi Wheat germ agglutinin-binding glycoproteins are decreased in Alzheimer's disease cerebrospinal fluid
    L R Fodero
    Department of Pathology, University of Melbourne, Victoria, Australia
    J Neurochem 79:1022-6. 2001
    ..The measurement of WGA-reactive glycoproteins in CSF may be a useful biomarker for diagnosis of Alzheimer's disease...
  2. ncbi Adsorptive endocytosis mediates the passage of HIV-1 across the blood-brain barrier: evidence for a post-internalization coreceptor
    W A Banks
    Veterans Affairs Medical Center and Tulane Univeristy School of Medicine, New Orleans, LA 70146, USA
    J Cell Sci 111:533-40. 1998
    ..This coreceptor probably contains a negatively charged heparin sulfate group and could be a member of the chemokine receptor family...
  3. pmc Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores
    D R Finlay
    J Cell Biol 104:189-200. 1987
    ..WGA is the first identified inhibitor of nuclear protein transport and interacts directly with the nuclear pore...
  4. ncbi A genetic approach to visualization of multisynaptic neural pathways using plant lectin transgene
    Y Yoshihara
    Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, Saitama, Japan
    Neuron 22:33-41. 1999
    ..This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system...
  5. pmc Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes
    Li A Kung
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06620 8103, USA
    Mol Syst Biol 5:308. 2009
    ....
  6. ncbi Binding, internalization, and membrane incorporation of human immunodeficiency virus-1 at the blood-brain barrier is differentially regulated
    W A Banks
    GRECC, Veterans Affairs Medical Center St Louis and St Louis University School of Medicine, Division of Geriatrics, Department of Internal Medicine, St Louis, MO 63106, USA
    Neuroscience 128:143-53. 2004
    ..In conclusion, binding, internalization, and membrane incorporation are separately regulated steps likely determining whether HIV-1 is incorporated into brain endothelial cells, transported across them, or returned to the circulation...
  7. ncbi Genetic tracing shows segregation of taste neuronal circuitries for bitter and sweet
    Makoto Sugita
    Department of Physiology and Oral Physiology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1 2 3, Minami Ku, Hiroshima 734 8553, Japan
    Science 309:781-5. 2005
    ....
  8. ncbi Evidence that the species barrier of human immunodeficiency virus-1 does not extend to uptake by the blood--brain barrier: comparison of mouse and human brain microvessels
    William A Banks
    GRECC, Veterans Affairs Medical Center St Louis and Saint Louis University School of Medicine, Division of Geriatrics, Department of Internal Medicine, St Louis, MO 63106, USA
    Life Sci 77:2361-8. 2005
    ..These results show that the mouse can be used to study the initial phases of HIV-1 uptake by the BBB...
  9. ncbi Tracking mouse visual pathways with WGA transgene
    Yoko Hanno
    Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Eur J Neurosci 18:2910-4. 2003
    ..Thus, this transgenic mouse line would provide a useful tool for analyzing phenotypic changes in the visual pathways of various mutant mice...
  10. ncbi Effects of lectins on CCK-8-stimulated enzyme secretion and differentiation of the rat pancreatic cell line AR42J
    U Mikkat
    Department of Pathology, Faculty of Medicine, University of Rostock, Strempelstrasse 14, D-18055 Rostock, Germany
    Pancreas 23:368-74. 2001
    ..CONCLUSION: In long-term studies, both lectins revealed no toxic or apoptosis-inducing effects. On the contrary, WGA showed an inhibitory effect on cell proliferation and led to improved differentiation of cells...
  11. ncbi Functionalisation of allergen-loaded microspheres with wheat germ agglutinin for targeting enterocytes
    Franziska Walter
    Department of Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, Vienna A 1090, Austria
    Biochem Biophys Res Commun 315:281-7. 2004
    ..Thus, we conclude that WGA-microspheres are suitable vehicles for oral delivery and mucosal targeting due to lectin-mediated bioadhesion...
  12. ncbi The lectin-cell interaction and its implications to intestinal lectin-mediated drug delivery
    Franz Gabor
    Institute of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A 1090 Vienna, Austria
    Adv Drug Deliv Rev 56:459-80. 2004
    ..As exemplified by lectin-grafted prodrug and carrier systems, this strategy is expected to improve absorption and probably bioavailability of poorly absorbable drugs, peptides and proteins as well as therapeutic DNA...
  13. ncbi Glycoconjugates on the surface of epididymal spermatozoa in a marsupial, the brushtail possum, Trichosurus vulpecula
    N J Cooper
    Department of Anatomical Sciences, Adelaide University, SA 5005, South Australia
    Reproduction 122:165-76. 2001
    ..It would appear that this situation differs markedly from that in sub-mammalian vertebrates...
  14. ncbi Collecting duct carcinoma mixed with common renal cell carcinoma: analysis of morphological characteristics using lectin histochemistry
    A Nagamoto
    Department of Urology, Yamato Municipal Hospital, Kanagawa, 242-8602, Japan
    Anticancer Res 21:567-70. 2001
    ..Based on the results of histological, histochemical and chromosomal examinations, we speculated on the histogenesis of this collecting duct carcinoma...
  15. ncbi Changes in the distribution of lectin receptors during capacitation and acrosome reaction in boar spermatozoa
    Irma Jiménez
    Departamento de Ciencias de la Salud, Universidad Autonoma Metropolitana Iztapalapa, Apdo Postal 55 535, México D F CP 09340, Mexico
    Theriogenology 59:1171-80. 2003
    ..This kind of study could improve the diagnosis of reproductive problems of subfertile boars and males of other species...
  16. ncbi A novel application of thermo-responsive polymer to affinity precipitation of polysaccharide
    Li Chun Pan
    Biomedical Engineering Center, Industrial Technology Research Institute, Chutung, Hsinchu 310, Taiwan, ROC
    J Biochem Biophys Methods 55:87-94. 2003
    ..Additionally, other affinity ligands could be coupled to the polymer for separation of the respective bioactive molecules...
  17. ncbi Approach to the comprehensive analysis of glycoproteins isolated from human serum using a multi-lectin affinity column
    Ziping Yang
    Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA
    J Chromatogr A 1053:79-88. 2004
    ....
  18. ncbi Characterization, fate, and function of hamster cortical granule components
    T Hoodbhoy
    Department of Biology, University of California, Riverside, California 92521, USA
    Mol Reprod Dev 58:223-35. 2001
    ....
  19. ncbi Binding and uptake of wheat germ agglutinin-grafted PLGA-nanospheres by caco-2 monolayers
    Andrea Weissenboeck
    Institute of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria
    Pharm Res 21:1917-23. 2004
    ..The Caco-2 association of lectin-grafted PLGA-nanospheres was investigated compared to plain and BSA-coated spheres...
  20. ncbi Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates
    Kazuki Nakajima
    Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3 4 1, Higashi Osaka 577 8502, Japan
    J Proteome Res 2:81-8. 2003
    ..The results will be useful, for example, to understand the biological events expressed with carbohydrate changes on the cell surface...
  21. ncbi Age-related alterations in the carbohydrate residue composition of the cell surface in the unexposed normal human epidermis
    S Georgiou
    Department of Dermatology, University of Patras, Greece
    Gerontology 51:155-60. 2005
    ....
  22. ncbi A topologically segregated one-bead-one-compound combinatorial glycopeptide library for identification of lectin ligands
    Laiqiang Ying
    Department of Chemistry, University of California, Davis, One Shields Ave, Davis, California 95616, USA
    J Comb Chem 7:372-84. 2005
    ....
  23. ncbi Relationships between degree of binding, cytotoxicity and cytoagglutinating activity of plant-derived agglutinins in normal lymphocytes and cultured leukemic cell lines
    Hideki Ohba
    Single Molecule Bioanalysis Laboratory, National Institute for Advanced Industrial Science and Technology, AIST Shikoku, 2217 14 Hayashi Cho, Takamatsu, 761 0395, Kagawa, Japan
    Cancer Chemother Pharmacol 51:451-8. 2003
    ..To clarify the relationships between the degree of lectin-cell binding, cytotoxicity and cytoagglutinating activity of plant-derived lectins in normal lymphocytes and cultured leukemic cell lines...
  24. ncbi Changes in oligosaccharide expression on plasma membrane of the mouse oocyte during fertilisation and early cleavage
    Kimiya Kitamura
    Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Zygote 11:183-9. 2003
    ....
  25. ncbi Wheat germ agglutinin-induced platelet activation via platelet endothelial cell adhesion molecule-1: involvement of rapid phospholipase C gamma 2 activation by Src family kinases
    T Ohmori
    Department of Laboratory Medicine, Yamanashi Medical University, Nakakoma, Yamanashi 409-3898, Japan
    Biochemistry 40:12992-3001. 2001
    ....
  26. ncbi Apoptosis induction associated with cell cycle dysregulation by rice bran agglutinin
    N Miyoshi
    Laboratory of Cellular Biochemistry, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada, Shizuoka 422-8526, Japan
    J Biochem 130:799-805. 2001
    ..These data indicate that RBA induces apoptosis associated with cell cycle arrest in U937 cells, and suggest that the induction mechanism for RBA is similar to that for WGA, but different from that for VAA...
  27. ncbi Prostaglandin D2 generation by rat peritoneal mast cells stimulated with Datura stramonium agglutinin and its inhibition by haptenic sugar and wheat germ agglutinin
    Tamiko Suzuki-Nishimura
    Department of Pharmacology, Meiji Pharmaceutical University, Tokyo, Japan
    Jpn J Pharmacol 90:77-80. 2002
    ..These results suggest that the binding of DSA to the corresponding sugar residues on the mast cell surface mediates the signaling of the prostaglandin G/H synthase pathway...
  28. ncbi Quantitative determination of dietary lectin activities by enzyme-linked immunosorbent assay using specific glycoproteins immobilized on microtiter plates
    Simone Vincenzi
    Dipartimento Scientifico e Tecnologico, Universita di Verona, Strada Le Grazie 15, 37134, Verona, Italy
    J Agric Food Chem 50:6266-70. 2002
    ..Examples of application to wheat germ and roasted peanut extracts are reported...
  29. ncbi A histopathological and lectin-histochemical study of the lining epithelium in postoperative maxillary cysts
    M Maruyama
    Department of Oral Medicine and Bioregulation, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan
    Oral Dis 8:241-8. 2002
    ..Histopathological and lectin-histochemical characteristics were studied in the lining epithelium of postoperative maxillary cysts (POMC)...
  30. ncbi Interaction of glycophorin A with lectins as measured by surface plasmon resonance (SPR)
    Bozena Krotkiewska
    Department of Medical Biochemistry, University Medical School, Wrocław, Poland
    Acta Biochim Pol 49:481-90. 2002
    ..The data obtained show that SPR is a valuable method to determine interaction of glycoproteins with lectins, which potentially can be used to detect differences in the carbohydrate moiety of individual glycoprotein samples...
  31. ncbi The influence of dietary lectins on the cell proliferation of human breast cancer cell lines in vitro
    Ursula Valentiner
    Institute for Anatomy II Experimental Morphology, University Hospital Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
    Anticancer Res 23:1197-206. 2003
    ..If used in cell culture, they can influence cellular proliferation. In this study the in vitro effects of six dietary lectins on the cell proliferation of human breast cancer cell lines were investigated...
  32. ncbi Hemocyte-mediated phagocytosis and melanization in the mosquito Armigeres subalbatus following immune challenge by bacteria
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Cell Tissue Res 313:117-27. 2003
    ..These studies implicate hemocytes as the primary line of defense against bacteria...
  33. pmc Comparison of alteration of cell surface carbohydrates of the chinchilla tubotympanum and colonial opacity phenotype of Streptococcus pneumoniae during experimental pneumococcal otitis media with or without an antecedent influenza A virus infection
    H H Tong
    Department of Otolaryngology, College of Medicine and Public Health, The Ohio State University, Columbus 43210, USA
    Infect Immun 70:4292-301. 2002
    ..pneumoniae on the changes of the carbohydrate moieties in the ET epithelium and that the selection of the opaque variant may facilitate the pneumococcal invasion of the middle ear...
  34. ncbi Human glands of Moll: histochemical and ultrastructural characterization of the glands of Moll in the human eyelid
    Mechthild Stoeckelhuber
    Anatomical Institute, Chair 2, University of Munich, Germany
    J Invest Dermatol 121:28-36. 2003
    ..We suppose that these apocrine glands are active from birth in producing agents against pathogenic microorganisms in the eyelid shaft and on the ocular surface...
  35. ncbi Lectin-sugar interaction. Calculated versus experimental binding energies
    Dirk Neumann
    Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbrucken, Germany
    Eur J Biochem 269:1518-24. 2002
    ..The insights gained from such theoretical studies may be used to complement the results from the still scarce crystal structures...
  36. ncbi Lectin binding in the human umbilical cord from normally grown pregnancies and from pregnancies complicated by intra-uterine growth retardation with absent or reversed diastolic flow
    E Sgambati
    Department of Anatomy, Histology and Forensic Medicine, University of Florence, Viale Morgagni 85, IT 50134 Florence, Italy
    Biol Neonate 84:119-34. 2003
    ..The reactivity with ConA and UEA I was stronger in the vein than in the arteries in both study groups. Some hypotheses are reported on the role played by the sugar residues in the normal and pathological umbilical cords...
  37. ncbi Lectin binding and endocytosis at the apical surface of human airway epithelia
    S M Yi
    Department of Otolaryngology, Head and Neck Surgery, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    Gene Ther 8:1826-32. 2001
    ..We found that several lectins bound to the apical surface were actively taken up into the cells. These data may be of value for studies of airway epithelial structure and may facilitate the targeting of the epithelial apical surface...
  38. ncbi Histochemistry of six lectins in the tissues of the flat fish Paralichthys olivaceus
    Kyung Sook Jung
    Department of Veterinary Medicine, Graduate School, Cheju National University, Jeju 690 756, Republic of Korea
    J Vet Sci 3:293-301. 2002
    ....
  39. ncbi Role of carbohydrates in repair of human respiratory epithelium using an in vitro model
    E C Adam
    Respiratory Cell and Molecular Biology Division, University of Southampton, Southampton, UK
    Clin Exp Allergy 33:1398-404. 2003
    ..The epithelial layer in the conducting airway provides a primary protective barrier. Repair of this barrier normally occurs rapidly after damage, but is compromised in diseases such as asthma...
  40. ncbi Infectivity of Chlorella species for the ciliate Paramecium bursaria is not based on sugar residues of their cell wall components, but on their ability to localize beneath the host cell membrane after escaping from the host digestive vacuole in the early
    Yuuki Kodama
    Department of Natural Science and Symbiosis, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi, Japan
    Protoplasma 231:55-63. 2007
    ....
  41. ncbi Differences in both glycosylation and binding properties between rat and mouse liver prolactin receptors
    O Lascols
    Laboratorie de Biologie Cellulaire, I N S E R M U402, Faculte de Medecine Saint Antoine, Paris, France
    Cell Mol Biol (Noisy-le-grand) 40:359-71. 1994
    ....
  42. ncbi Human FSH isoforms: carbohydrate complexity as determinant of in-vitro bioactivity
    S Creus
    , Gallo 1330. C.P. 1425, Buenos Aires, Argentina
    Mol Cell Endocrinol 174:41-9. 2001
    ....
  43. ncbi Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution
    Alex Monzo
    Horvath Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 66, 6020, Innsbruck, Austria
    Anal Bioanal Chem 389:2097-102. 2007
    ..Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution...
  44. ncbi Lectin binding and uptake in human (myelo)monocytic cell lines: HL60 and U937
    U Schumacher
    University of Southampton, United Kingdom
    Ultrastruct Pathol 20:463-71. 1996
    ....
  45. ncbi Changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding
    S Kirkeby
    Institute of Anatomy, Royal Dental College of Copenhagen, Denmark
    Pathobiology 60:297-302. 1992
    ..These changes of the muscle carbohydrate components might be involved in the expression of the dystrophic syndrome This seems to be the first report on changes of soluble glycoproteins in muscular dystrophy...
  46. pmc Identification and characterization of the major stilbene-disulphonate- and concanavalin A-binding protein of the porcine renal brush-border membrane as aminopeptidase N
    H See
    Department of Medicine, University of Toronto, Canada
    Biochem J 271:147-55. 1990
    ..Bicarbonate and iodide blocked the binding of GP 130 to the SITS-affinity resin, showing that GP 130 has an anion-binding site. Neither these anions nor stilbene disulphonates affected the aminopeptidase N activity of GP 130...
  47. ncbi Boronic acid lectin affinity chromatography (BLAC). 2. Affinity micropartitioning-mediated comparative glycosylation profiling
    Alex Monzo
    Horvath Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 66, Innsbruck, Austria
    Anal Bioanal Chem 392:195-201. 2008
    ....
  48. pmc Analysis of unconventional approaches for the rapid detection of surface lectin binding ligands on human cell lines
    Lily Anne Y Welty
    Department of Biology and The Center for Cancer and Developmental Biology, California State University Northridge, 18111 Nordhoff Street, Northridge, CA 91330 8303, USA
    Acta Histochem 107:411-20. 2006
    ..The direct use of frozen, thawed and fixed cells allows rapid mass screening for surface molecules, without the requirement for costly and time consuming cell culture...
  49. ncbi Effect of lysozyme on plasma membranes of liver and heart cells: lectin binding assay
    M V Mikaelyan
    Institute of Biochemistry, Physical Chemistry of Proteins, National Academy of Sciences, P Sevak str 5 1, Yerevan 375014, Armenia
    Cell Biol Int 31:699-702. 2007
    ..The investigations were carried out on polyacrylamide slices immobilized with wheat germ agglutinin or Concanavalin A. It was shown that lysozyme changes the carbohydrate profile of plasma membranes of liver and heart cells...
  50. ncbi Lectin-induced aggregates of blood cells from patients with acute coronary syndromes
    Irina V Gorudko
    Department of Biophysics, Belarusian State University, Minsk, Belarus
    Arch Med Res 39:674-81. 2008
    ....
  51. ncbi Effect of intraperitoneally administered plant lectins on leukocyte diapedesis and visceral organ weight in rats and mice
    Karoly Baintner
    Department of Physiology, Faculty of Animal Science, University of Kaposvar, Kaposvar, H 7401, Hungary
    Pathol Oncol Res 13:139-43. 2007
    ..The experiments show that plant lectins, used as models of bacterial lectins, can reproduce some aspects of peritonitis...
  52. ncbi Characterization of the non-sulphated highly anionic Kurloff cell glycoconjugates by lectin- and immunoblotting: evidence for the presence of alpha (2,8)-linked polysialic acid-bearing molecules
    S E Letaïef
    Laboratoire d Histologie, Universite de Caen, CHU Cote de Nacre, France
    Biol Cell 77:269-76. 1993
    ..Moreover, bindings to Phaseolus vulgaris and to wheat germ agglutinins suggest the presence of bisecting N-acetylglucosamine residues...
  53. ncbi Location of the carbohydrates present in the HK-ATPase vesicles isolated from hog gastric mucosa
    K Hall
    CURE, Veterans Administration Medical Center, Los Angeles, California 90073
    Biochemistry 29:701-6. 1990
    ..Electron microscopy using postembedding staining techniques showed that both concanavalin A and wheat germ agglutinin staining occurred on the extracellular face of the gastric vesicles.(ABSTRACT TRUNCATED AT 250 WORDS)..
  54. ncbi Distribution of postganglionic parasympathetic fibers originating in the pterygopalatine ganglion in the maxillary and ophthalmic nerve branches of the trigeminal nerve; HRP and WGA-HRP study in the guinea pig
    L A Segade
    Department of Morphological Sciences, Faculty of Medicine, University of Santiago de Compostela, Spain
    Brain Res 522:327-32. 1990
    ..The presence of PTPG fibers in the supraorbital, infratrochlear, zygomaticotemporal, zygomaticofacial-inferior palpebral, sphenopalatine and infraorbital-superior alveolar nerves has not hitherto been reported in mammals...
  55. ncbi Purification of murine hemopoietic stem cells and committed progenitors by fluorescence activated cell sorting using wheat germ agglutinin and monoclonal antibodies
    J G Bauman
    Radiobiological Institute, TNO, Rijswijk, The Netherlands
    Acta Histochem Suppl 36:241-53. 1988
    ..This multicolour analysis and sorting therefore is equivalent to the multistep sorting procedures...
  56. ncbi Golgi apparatus and TGN during endocytosis
    Monika Vetterlein
    Institute of Histology and Embryology, Department of Cell Biology and Ultrastructure Research, University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria
    Histochem Cell Biol 117:143-50. 2002
    ..The findings suggest that incorporation of TGN elements into Golgi stacks provides a mechanism for uptake of internalised WGA into the Golgi apparatus...
  57. ncbi Cellular localization and characterization of proteins that bind high density lipoprotein
    B Hokland
    Department of Medicine, University of Washington, Seattle 98195
    J Lipid Res 33:1335-42. 1992
    ..The cellular localization and physical properties of the 110 and 130 kDa proteins suggest that they may play a role in binding of HDL to the cell surface...
  58. pmc A flow-cytometric gram-staining technique for milk-associated bacteria
    Claus Holm
    Department of Veterinary and Milk Quality, Danish Dairy Board, DK 8000 Aarhus C, Denmark
    Appl Environ Microbiol 69:2857-63. 2003
    ..These results demonstrate that the technique is suitable for analyzing milk samples without precultivation...
  59. ncbi Visualizing the endocytic and exocytic processes of wheat germ agglutinin by quantum dot-based single-particle tracking
    Shu Lin Liu
    Key Laboratory of Analytical Chemistry for Biology and Medicine Ministry of Education, College of Chemistry and Molecular Sciences, Research Center for Nanobiology and Nanomedicine MOE 985 Innovative Platform, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, PR China
    Biomaterials 32:7616-24. 2011
    ..This is important for the application of lectins as drug carriers and antineoplastic drugs in medicine, and also offers insights into the pathway of endocytosis and exocytosis...
  60. ncbi Glycoconjugates of choroidal neovascular membranes in age-related macular degeneration
    Robert F Mullins
    Center for Macular Degeneration, Department of Ophthalmology and Visual Science, The University of Iowa Carver College of Medicine, Iowa City, IA, USA
    Mol Vis 11:509-17. 2005
    ..The purpose of this study was to characterize the carbohydrate groups associated with CNV by lectin histochemistry...
  61. ncbi Translocation of beta-catenin into the nucleus independent of interactions with FG-rich nucleoporins
    Eun Kyung Suh
    Neuroscience Program, Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Exp Cell Res 290:447-56. 2003
    ..Transport is blocked by a soluble form of the C-cadherin cytoplasmic domain, suggesting that masking of the nuclear targeting signal may be a mechanism of regulating beta-catenin subcellular localization...
  62. ncbi [Preparation of lectin-conjugated PLGA nanoparticles and evaluation of their in vitro bioadhesive activity]
    Ya Shu Yin
    School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
    Yao Xue Xue Bao 42:550-6. 2007
    ....
  63. ncbi Cell-cell contact and membrane spreading in an ultrasound trap
    W T Coakley
    School of Biosciences, Main Building, Cardiff University, Cardiff CF10 3TL, UK
    Colloids Surf B Biointerfaces 34:221-30. 2004
    ..The potential of the technique to characterise and control the progression of cell adhesion in suspension away from solid substrata is discussed...
  64. ncbi Stabilization of extracellular ascorbate mediated by coenzyme Q transmembrane electron transport
    Antonio Arroyo
    Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide, Seville, Spain
    Methods Enzymol 378:207-17. 2004
  65. ncbi Comparative glycoproteomics based on lectins affinity capture of N-linked glycoproteins from human Chang liver cells and MHCC97-H cells
    Zhibin Xu
    Glycoconjugate Key Laboratory, Ministry of Health, Department of Biochemistry and Molecular Biology, Fudan University, Shanghai, PR China
    Proteomics 7:2358-70. 2007
    ..Our work was part of the HUPO Human Liver Proteome Project (HLPP) studies and was supported by CHINA HUPO...
  66. pmc Changes in the organization of excitation-contraction coupling structures in failing human heart
    David J Crossman
    Department of Physiology, Faculty of Medicine and Health Sciences, University of Auckland, Auckland, New Zealand
    PLoS ONE 6:e17901. 2011
    ....
  67. ncbi NMR and molecular modeling studies of the interaction between wheat germ agglutinin and the beta-D-GlcpNAc-(1-->6)-alpha-D-Manp epitope present in glycoproteins of tumor cells
    Kristina Lycknert
    Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S 106 91 Stockholm, Sweden
    Biochemistry 43:9647-54. 2004
    ..Experimental and theoretical data therefore agree that one conformation with a gt conformation of the hydroxymethyl group and a negative sign for the psi torsion angle is indeed selected by the lectin upon binding...
  68. ncbi Proteomic characterization of isolated retinal pigment epithelium microvilli
    Vera L Bonilha
    The Cole Eye Institute, Department of Ophthalmic Research, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Mol Cell Proteomics 3:1119-27. 2004
    ..The isolation technique used should be amenable for isolating microvilli in other epithelia as well, allowing new insights into additional functions of this important epithelial compartment...
  69. ncbi Bioadhesion and enhanced bioavailability by wheat germ agglutinin-grafted lipid nanoparticles for oral delivery of poorly water-soluble drug bufalin
    Ying Liu
    Department of Pharmaceutics, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201003, PR China
    Int J Pharm 419:260-5. 2011
    ..7-fold improvement in oral bioavailability. These results illustrate the potential utility of WGA-grafted lipid nanoparticles for oral delivery of a poorly water-soluble drug such as bufalin...
  70. ncbi [Wheat germ agglutinin anchored chitosan nanoparticles and its conjugation with N-acetylglucosamine]
    Hui Li
    Department of Pharmaceutics, Shanghai Eighth People s Hospital, Shanghai 200235, China
    Yao Xue Xue Bao 47:131-4. 2012
    ..After the addition of NAG, the binding rates between CS-NPs and BSM almost didn't change, while the binding rates between WGA-CS-NPs and BSM dropped down significantly, which confirmed the specific binding characteristics of WGA to NAG...
  71. ncbi Exploring the binding profiles of ConA, boronic acid and WGA by MALDI-TOF/TOF MS and magnetic particles
    Katrin Sparbier
    Bruker Daltonik GmbH, Permoserstrasse 15, 04318 Leipzig, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 840:29-36. 2006
    ..Search results revealed overlapping profiles of known serum glycoproteins...
  72. ncbi In vitro and in vivo evaluation of WGA-carbopol modified liposomes as carriers for oral peptide delivery
    Abdallah Makhlof
    Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University, Gifu, Japan
    Eur J Pharm Biopharm 77:216-24. 2011
    ....
  73. ncbi The bud scar-based screening system for hunting human genes extending life span
    Cuiying Chen
    Fundamental and Applied Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, Technologiepark 927, B 9052 Zwijnaarde, Belgium
    Ann N Y Acad Sci 1019:355-9. 2004
    ..The knowledge gained from the studies of budding yeast will benefit studies in humans for better understanding of aging and aging-related disease...
  74. ncbi The wheat germ agglutinin-fractionated proteome of subjects with Alzheimer's disease and mild cognitive impairment hippocampus and inferior parietal lobule: Implications for disease pathogenesis and progression
    Fabio Di Domenico
    Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
    J Neurosci Res 88:3566-77. 2010
    ..This study, the first to use proteomics to identify WGA-fractionated proteins isolated from brains from subjects with MCI and AD, provides additional information about the active proteome of the brain throughout AD progression...
  75. ncbi Preparation of a high-performance multi-lectin affinity chromatography (HP-M-LAC) adsorbent for the analysis of human plasma glycoproteins
    Majlinda Kullolli
    Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA
    J Sep Sci 31:2733-9. 2008
    ..A single clinical sample can be fractionated in less than 10 min per run, making this a useful sample preparation tool for proteomics/glycoproteomics studies associated with disease abnormalities...
  76. ncbi Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 303:1010-4. 2004
    ..We propose that when p53 accumulates in the cytosol, it can function analogously to the BH3-only subset of proapoptotic Bcl-2 proteins to activate Bax and trigger apoptosis...
  77. ncbi Surface modification of liposomes using polymer-wheat germ agglutinin conjugates to improve the absorption of peptide drugs by pulmonary administration
    Mitsutaka Murata
    Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University, Gifu 501 1196, Japan
    J Pharm Sci 102:1281-9. 2013
    ..Hence, surface modification of liposomes with CP-WGA has potential for effective pulmonary administration of peptides...
  78. ncbi Histochemical characterisation of trigeminal neurons that innervate monkey extraocular muscles
    K Fackelmann
    Institute of Anatomy, Ludwig Maximilian University of Munich, Munich, Germany
    Prog Brain Res 171:17-20. 2008
    ..A surprisingly large population of NOS-positive TG cells (30%) was found only after distal tracer injections. Up to now none of these TG cell groups could be related to the palisade endings located at the myotendinous junction...
  79. ncbi Adjuvant effect of Gantrez®AN nanoparticles during oral vaccination of piglets against F4+enterotoxigenic Escherichia coli
    Katrien Vandamme
    Ghent University, Laboratory of Pharmaceutical Technology, Harelbekestraat 72, B 9000 Ghent, Belgium
    Vet Immunol Immunopathol 139:148-55. 2011
    ..These data indicate that nanoparticle effects after F4 encapsulation were of lesser importance for the adjuvant effect of Gantrez(®)AN NP, contrarily to the reactivity of the Gantrez(®)AN polymer used to prepare the nanoparticles...
  80. ncbi Mu-opioid and N-methyl-D-aspartate receptors are localized at laryngeal motoneurons of guinea pigs
    Hiromi Yamazaki
    Department of Molecular and Medical Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
    Biol Pharm Bull 32:293-6. 2009
    ..The present study demonstrates phenotypic variation in the synaptic transmission to the LMNs in guinea pigs. These results suggest modulation of the glottic movement by mu-opioid and NMDA mechanisms...
  81. ncbi Glycoproteomic analysis of WGA-bound glycoprotein biomarkers in sera from patients with lung adenocarcinoma
    Piyorot Hongsachart
    Institute of Biological Chemistry and Genomics Research Center, Academia Sinica, Taipei, Taiwan
    Electrophoresis 30:1206-20. 2009
    ..We suggest that these altered glycoproteins may be useful as biomarkers for lung cancer development and progression...
  82. ncbi Wheat germ agglutinin-conjugated chitosan-Ca-alginate microparticles for local colon delivery of 5-FU: development and in vitro characterization
    M Glavas Dodov
    Institute of Pharmaceutical Technology, Faculty of Pharmacy, University Ss Cyril and Methodious, Vodnjanska 17, P O Box 36, 1000 Skopje, Macedonia
    Int J Pharm 381:166-75. 2009
    ..Functionalized MPs showed excessive mucoadhesiveness in vitro, due to the positive surface charge, pH-dependent swelling of the matrix and lectin-sugar recognition...
  83. ncbi Affinity monolithic capillary columns for glycomics/proteomics: 1. Polymethacrylate monoliths with immobilized lectins for glycoprotein separation by affinity capillary electrochromatography and affinity nano-liquid chromatography in either a single colum
    Fred M Okanda
    Department of Chemistry, Oklahoma State University, Stillwater, OK, USA
    Electrophoresis 27:1020-30. 2006
    ..e., tandem columns) for enhanced separation of glycoproteins by LAC using the CEC modality. Furthermore, in the coupled columns format, glycoforms of a given glycoprotein were readily separated...
  84. ncbi Import of plasmid DNA into the nucleus is sequence specific
    D A Dean
    Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688, USA
    Exp Cell Res 230:293-302. 1997
    ..Taken together, these results support a model in which plasmid DNA nuclear import occurs by a mechanism similar to that used by nuclear localization signal-containing proteins but is also dependent on transcription...
  85. ncbi Cholesterol depletion by methyl-beta-cyclodextrin blocks cholera toxin transport from endosomes to the Golgi apparatus in hippocampal neurons
    H Shogomori
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel
    J Neurochem 78:991-9. 2001
    ..We conclude that CT transport from endosomes to the Golgi apparatus is cholesterol-dependent, whereas CT transport from the Golgi apparatus to lysosomes is cholesterol-independent...
  86. ncbi TrkB dimerization during development of the prefrontal cortex of the macaque
    K Ohira
    Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Kanrin, Inuyama, Aichi, Japan
    J Neurosci Res 65:463-9. 2001
    ..Moreover, the intercellular binding proteins of TrkB were not cross-linked by BS3. Therefore, these results suggest that the change in dimerization among TrkB subtypes occurs during development of the monkey prefrontal cortex...
  87. ncbi Cytotactin and its proteoglycan ligand mark structural and functional boundaries in somatosensory cortex of the early postnatal mouse
    K L Crossin
    Rockefeller University, New York, New York 10021 6399
    Dev Biol 136:381-92. 1989
    ..were recognized by lentil lectin and concanavalin A and CTB proteoglycan was also recognized by peanut and wheat germ agglutinins. The HNK-1 carbohydrate antigen, present on cytotactin, CTB proteoglycan, and other adhesion molecules, ..
  88. ncbi Early events of secretory granule formation in the rat parotid acinar cell under the influence of isoproterenol. An ultrastructural and lectin cytochemical study
    F D'Amico
    Centro di Microscopia Elettronica di Medicina, University of Catania, Italy
    Eur J Histochem 45:169-75. 2001
    ..Immature and mature secretory granules were labelled with WGA, SBA, PNA, and lightly with UEA-I. Cis and intermediate cisternae of the Golgi apparatus were labelled with WGA, and trans cisternae with WGA and SBA...
  89. ncbi Localization of N-methyl-D-aspartate NR2B subunits on primary sensory neurons that give rise to small-caliber sciatic nerve fibers in rats
    Q P Ma
    Department of Pharmacology, Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Terlings Park, CM20 2QR, Harlow, UK
    Neuroscience 101:699-707. 2000
    ....
  90. ncbi Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8-aminonaphthalene-1,3,6-trisulfonate
    F Y Che
    Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, PRC
    Biotechniques 30:1272-6, 1278. 2001
    ....
  91. ncbi Epithelial polarity: the ins and outs of the fly epidermis
    J A Knoblich
    Research Institute of Molecular Pathology I M P, Vienna, Austria
    Curr Biol 10:R791-4. 2000
    ..Recent experiments have shed light on how apical-basal polarity is generated during cellularization in Drosophila, when around 6000 epithelial cells are created synchronously from a syncytium...
  92. ncbi Purification of rat C6 glioma plasma membranes by affinity partitioning
    Q Wang
    Department of Biochemistry, University of Antwerp, Universitaire Instelling Antwerpen, Antwerp, Belgium
    Biol Signals Recept 9:45-52. 2000
    ..Furthermore, the isolated plasma membranes retain their functional integrity, as shown by the high-affinity insulin-like growth factor-I (IGF-I) binding capacity of IGF-I receptors...
  93. ncbi Functional and anatomical organization of cardiovascular pressor and depressor sites in the lateral hypothalamic area. II. Ascending projections
    G V Allen
    Robarts Research Institute, London, Ontario, Canada
    J Comp Neurol 330:421-38. 1993
    ..The results demonstrate that the posterolateral hypothalamus of the rat contains two regions with specific cardiovascular function and highly organized connections with diencephalic, forebrain, and cortical structures...
  94. ncbi The influence of a colonic microbiota on HPMA copolymer lectin conjugates binding in rodent intestine
    S Wroblewski
    Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA
    J Drug Target 9:85-94. 2001
    ..In GF animals, luminal surface binding of P(HPMA)-(WGA-FITC) was visible, and goblet cell binding of P(HPMA)-(PNA-FITC) was seen. These subtle changes suggest that altered glycoprotein expression occurred under GF conditions...
  95. ncbi Identification of a conserved loop in Mog1 that releases GTP from Ran
    S M Steggerda
    Center for Cell Signaling, Department of Biochemistry and Molecular Genetics, and Cell and Molecular Biology Program, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Traffic 2:804-11. 2001
    ..These data suggest that Mog1 and RanGEF use similar mechanisms to facilitate guanine nucleotide release from Ran...
  96. ncbi Reduction of spontaneous metastases through induction of carbohydrate cross-reactive apoptotic antibodies
    Behjatolah Monzavi-Karbassi
    Arkansas Cancer Research Center and Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, 72205, USA
    J Immunol 174:7057-65. 2005
    ..This finding parallels suggestions that carbohydrate-reactive IgM with apoptotic activity may have merit in the adjuvant setting if the right carbohydrate-associated targets are identified...
  97. ncbi Canine serum alkaline phosphatase isoenzymes detected by polyacrylamide gel disk electrophoresis
    Hiroshi Itoh
    Department of Veterinary Surgery, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan
    J Vet Med Sci 64:35-9. 2002
    ....
  98. ncbi Somatotopic distribution of corneal afferent neurons in the guinea pig trigeminal ganglion
    J T Keller
    Christ Hospital, Department of Neurosurgery, Cincinnati, OH
    Neurosci Lett 121:247-50. 1991
    ..The results of this study have provided for the first time the precise location and somata diameter of primary afferent corneal neurons within the guinea pig trigeminal ganglion...
  99. ncbi Bioengineering single crystal growth
    Ching Hsuan Wu
    Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, United States
    J Am Chem Soc 133:1658-61. 2011
    ..This allows us to control the placement and orientation of smooth, cylindrical calcite single crystals where the crystallographic c-direction is parallel to the cylinder axis and the underlying line pattern...
  100. ncbi A flow cytometric method for viability assessment of Staphylococcus aureus and Burkholderia cepacia in mixed culture
    Marc Rüger
    Chair of Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany
    Cytometry A 81:1055-66. 2012
    ....
  101. pmc Anti-(insulin receptor) monoclonal antibody-stimulated tyrosine phosphorylation in cells transfected with human insulin receptor cDNA
    N P Brindle
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 268:615-20. 1990
    ..These data are consistent with a role for the insulin receptor tyrosine kinase activity in the mechanism of action of insulin-mimetic anti-receptor antibodies...

Research Grants11

  1. MEMBRANE ANTIGENS OF RAT PROSTATE ADENOCARCINOMA
    ALICE CLAFLIN; Fiscal Year: 1980
    ..Purified membrane preparations from the H tumor bind more concanavalin A (Con A) and Wheat Germ Agglutinins than do G tumor membrane preparations...
  2. Opiate Addiction and HIV-1 Induced Release of Cytokines
    William Banks; Fiscal Year: 2006
    ....
  3. Prion Transport Across the Blood-Brain Barrier
    William Banks; Fiscal Year: 2009
    ..To cause disease, prions must cross the blood-brain barrier to enter the brain. We will determine how prions cross the BBB. Knowing how prions enter the brain should lead to strategies on how to prevent prion diseases. ..
  4. Mechanisms of HIV Transport Across the BBB
    William Banks; Fiscal Year: 2004
    ....
  5. PTS1 Regulation of Brain Met Enk Levels in Alcoholism
    William Banks; Fiscal Year: 2004
    ..abstract_text> ..
  6. Determining Therapeutic Efficacy of AGE in AD
    Neelima Chauhan; Fiscal Year: 2005
    ..If successful, this project will validate the use of safe, naturally well-tolerated, cost-effective and alternative herbal pharmacotherapy for treating AD. ..
  7. Reduction of Amyloid Burden by Antisense APP
    Neelima Chauhan; Fiscal Year: 2005
    ..Significance: Targeting the translation of amyloidogenic APP by antisense synthetic oligodeoxynucleotides may prove to be an effective and safe form of gene therapy for treating familial AD and other genetic diseases. ..
  8. Pathobiology of HMG-CoA reductase inhibitors in diabetes
    Yashpal Kanwar; Fiscal Year: 2006
    ....
  9. Dendritic cell function and ethanol
    ANNETTE SCHLUETER; Fiscal Year: 2007
    ..This in turn will elucidate new approaches for enhancing immunity and preventing infection in alcoholics, as the appropriate points of intervention become clear. ..
  10. GLOMERULAR CAPILLARY WALL--NORMAL AND PATHOLOGIC
    Yashpal Kanwar; Fiscal Year: 2008
    ..Null alleles will be interbred to generate Null -/- mutant as a conventional KO mice with ablation of the TIN-ag gene. ..
  11. The Choriocapillaris in Aging and Macular Disease
    Robert Mullins; Fiscal Year: 2005
    ..These experiments are expected to provide novel insights into the potential role of the choriocapillaris in the pathobiology of AMD. ..