immediate early proteins

Summary

Summary: Proteins that are coded by immediate-early genes, in the absence of de novo protein synthesis. The term was originally used exclusively for viral regulatory proteins that were synthesized just after viral integration into the host cell. It is also used to describe cellular proteins which are synthesized immediately after the resting cell is stimulated by extracellular signals.

Top Publications

  1. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
  2. pmc Concurrent expression of latent and a limited number of lytic genes with immune modulation and antiapoptotic function by Kaposi's sarcoma-associated herpesvirus early during infection of primary endothelial and fibroblast cells and subsequent decline of l
    Harinivas H Krishnan
    Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, Kansas, USA
    J Virol 78:3601-20. 2004
  3. ncbi (Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms
    Florian Lang
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Physiol Rev 86:1151-78. 2006
  4. pmc Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a)
    A Brunet
    Division of Neuroscience, Children s Hospital and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 21:952-65. 2001
  5. ncbi Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway
    J P Rouault
    Unité INSERM U453, affiliée au CNRS, Centre Leon Berard, Lyon, France
    Nat Genet 14:482-6. 1996
  6. ncbi MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo
    H Sun
    Cold Spring Harbor Laboratory, New York 11724 2208
    Cell 75:487-93. 1993
  7. pmc Herpes simplex virus ICP0 promotes both histone removal and acetylation on viral DNA during lytic infection
    Anna R Cliffe
    Harvard Medical School, Department of Microbiology and Molecular Genetics, 200 Longwood Avenue, Boston, MA 02115, USA
    J Virol 82:12030-8. 2008
  8. ncbi Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Science 281:1001-5. 1998
  9. pmc Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum
    M K Webster
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Mol Cell Biol 13:2031-40. 1993
  10. pmc PML contributes to a cellular mechanism of repression of herpes simplex virus type 1 infection that is inactivated by ICP0
    Roger D Everett
    MRC Virology Unit, Church Street, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 80:7995-8005. 2006

Detail Information

Publications278 found, 100 shown here

  1. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
    ..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
  2. pmc Concurrent expression of latent and a limited number of lytic genes with immune modulation and antiapoptotic function by Kaposi's sarcoma-associated herpesvirus early during infection of primary endothelial and fibroblast cells and subsequent decline of l
    Harinivas H Krishnan
    Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, Kansas, USA
    J Virol 78:3601-20. 2004
    ..These are unique observations among in vitro herpesvirus infections and may have important implications in KSHV biology and pathogenesis...
  3. ncbi (Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms
    Florian Lang
    Department of Physiology, University of Tuebingen, Tuebingen, Germany
    Physiol Rev 86:1151-78. 2006
    ..There is little doubt that further targets will be identified that are modulated by the SGK isoforms and that further SGK-dependent in vivo physiological functions and pathophysiological conditions will be defined...
  4. pmc Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a)
    A Brunet
    Division of Neuroscience, Children s Hospital and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 21:952-65. 2001
    ..These findings indicate that SGK acts in concert with Akt to propagate the effects of PI3K activation within the nucleus and to mediate the biological outputs of PI3K signaling, including cell survival and cell cycle progression...
  5. ncbi Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway
    J P Rouault
    Unité INSERM U453, affiliée au CNRS, Centre Leon Berard, Lyon, France
    Nat Genet 14:482-6. 1996
    ..7). Here, we cloned and localized the human BTG2 gene. We show that BTG2 expression is induced through a p53-dependent mechanism and that BTG2 function may be relevant to cell cycle control and cellular response to DNA damage...
  6. ncbi MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo
    H Sun
    Cold Spring Harbor Laboratory, New York 11724 2208
    Cell 75:487-93. 1993
    ..The mutant 3CH134 protein also forms a physical complex with the phosphorylated form of p42MAPK. These findings suggest that 3CH134 is a physiological MAP kinase phosphatase; we propose the name MKP-1 for this phosphatase...
  7. pmc Herpes simplex virus ICP0 promotes both histone removal and acetylation on viral DNA during lytic infection
    Anna R Cliffe
    Harvard Medical School, Department of Microbiology and Molecular Genetics, 200 Longwood Avenue, Boston, MA 02115, USA
    J Virol 82:12030-8. 2008
    ..This study provides evidence that ICP0 directly alters the chromatin structure of the HSV-1 genome during lytic infection, and this system will serve as a useful model for the reduction of histone load in higher eukaryotes...
  8. ncbi Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Science 281:1001-5. 1998
    ..These findings identify TTP as a component of a negative feedback loop that interferes with TNF-alpha production by destabilizing its messenger RNA. This pathway represents a potential target for anti-TNF-alpha therapies...
  9. pmc Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum
    M K Webster
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Mol Cell Biol 13:2031-40. 1993
    ....
  10. pmc PML contributes to a cellular mechanism of repression of herpes simplex virus type 1 infection that is inactivated by ICP0
    Roger D Everett
    MRC Virology Unit, Church Street, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 80:7995-8005. 2006
    ..We conclude that PML contributes to a cellular antiviral repression mechanism that is countered by the activity of ICP0...
  11. pmc Serum and glucocorticoid-inducible kinase (SGK) is a target of the PI 3-kinase-stimulated signaling pathway
    J Park
    Friedrich Miescher Institut, Maulbeerstrasse 66, CH 4056 Basel, Switzerland
    EMBO J 18:3024-33. 1999
    ..Both hyperphosphorylation and nuclear translocation could be inhibited by wortmannin, but not by rapamycin...
  12. pmc A viral protein that selectively downregulates ICAM-1 and B7-2 and modulates T cell costimulation
    L Coscoy
    Howard Hughes Medical Institute and Departments of Microbiology and Medicine, University of California Medical Center, San Francisco, California 94143-0414, USA
    J Clin Invest 107:1599-606. 2001
    ..We propose that its expression reduces T cell responses to KSHV-infected B cells early in infection, thereby diminishing antiviral cytokine release and the production of stimulatory signals for CTL generation...
  13. ncbi Matrix metalloproteinases cleave connective tissue growth factor and reactivate angiogenic activity of vascular endothelial growth factor 165
    Gakuji Hashimoto
    Department of Pathology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
    J Biol Chem 277:36288-95. 2002
    ..MMPs may play a novel role through CTGF degradation in VEGF-induced angiogenesis during embryonic development, tissue maintenance, and/or pathological processes of various diseases...
  14. pmc Global changes in Kaposi's sarcoma-associated virus gene expression patterns following expression of a tetracycline-inducible Rta transactivator
    Hiroyuki Nakamura
    Department of Microbiology and Molecular Genetics, Division of Tumor Virology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA
    J Virol 77:4205-20. 2003
    ..Thus, our streamlined gene expression system provides a novel means not only to study the effects of viral gene products on overall KSHV gene expression and replication, but also to understand the natural viral reactivation process...
  15. pmc Molecular cloning of PC3, a putatively secreted protein whose mRNA is induced by nerve growth factor and depolarization
    A Bradbury
    Istituto di Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy
    Proc Natl Acad Sci U S A 88:3353-7. 1991
    ..5, placenta, amnion, and spleen, which are proliferating and/or differentiating. The deduced protein sequence from the cDNA indicates the presence of a signal peptide, suggesting that PC3 is secreted...
  16. pmc Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2
    T Kobayashi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 339:319-28. 1999
    ..These findings raise the possibility that some physiological roles ascribed to PKB on the basis of the overexpression of constitutively active PKB mutants might be mediated by SGK...
  17. pmc ICP27 interacts with the RNA export factor Aly/REF to direct herpes simplex virus type 1 intronless mRNAs to the TAP export pathway
    I Hsiung Brandon Chen
    Department of Microbiology and Molecular Genetics, Medical Sciences I, College of Medicine, University of California, Irvine, CA 92697 4025, USA
    J Virol 76:12877-89. 2002
    ..Interestingly, the leucine-rich N-terminal sequence was required for efficient export, even though ICP27 export was LMB insensitive. Thus, this region is required for efficient ICP27 export but does not function as a CRM1-dependent NES...
  18. ncbi In search of a function for the TIS21/PC3/BTG1/TOB family
    S Matsuda
    Department of Molecular Pathogenesis, Nagoya University School of Medicine, Japan
    FEBS Lett 497:67-72. 2001
    ..It is now emerging that every member of this family has a potential to regulate cell growth. We would like to propose here to use a nomenclature APRO as a new term for the family...
  19. pmc Human cytomegalovirus immediate-early 1 protein facilitates viral replication by antagonizing histone deacetylation
    Michael Nevels
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    Proc Natl Acad Sci U S A 101:17234-9. 2004
    ..We also demonstrate an interaction between IE2 and HDAC3. We propose that the ability to modify chromatin is fundamental to transcriptional activation by IE1 and, likely, IE2 as well...
  20. pmc Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells
    Torsten Bunde
    Institut fur Medizinische Immunologie, Charite, Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    J Exp Med 201:1031-6. 2005
    ..In conclusion, high frequencies of IE-1 but not pp65-specific CD8 T cells correlate with protection from CMV disease. These results have important implications for monitoring T cell responses, adoptive cell therapy, and vaccine design...
  21. pmc Chromatin remodeling of the Kaposi's sarcoma-associated herpesvirus ORF50 promoter correlates with reactivation from latency
    Fang Lu
    The Wistar Institute Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 77:11425-35. 2003
    ..Together, these results suggest that remodeling of a stably positioned nucleosome at the transcriptional initiation site of ORF50 is a regulatory step in the transition from latent to lytic infection...
  22. pmc Glucocorticoids inhibit MAP kinase via increased expression and decreased degradation of MKP-1
    O Kassel
    Forschungszentrum Karlsruhe, Institute of Toxicology and Genetics, PO Box 3640, D-76021 Karlsruhe, Germany
    EMBO J 20:7108-16. 2001
    ..These results identify MKP-1 as essential for glucocorticoid-mediated control of Erk-1/2 activation and unravel a novel regulatory mechanism for this anti-inflammatory drug...
  23. ncbi Connective tissue growth factor is a mediator of angiotensin II-induced fibrosis
    Monica Ruperez
    Vascular and Renal Research Laboratory, Autonoma University, Fundacion Jimenez Diaz, Avda Reyes Catolicos, 2, 28040 Madrid, Spain
    Circulation 108:1499-505. 2003
    ..Angiotensin II (Ang II) participates in the development of fibrosis during vascular damage. Connective tissue growth factor (CTGF) is a novel fibrotic mediator. However, the potential link between CTGF and Ang II has not been investigated...
  24. pmc Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus
    B Sodeik
    Yale University School of Medicine, Department of Cell Biology, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 136:1007-21. 1997
    ..We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus...
  25. ncbi Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts
    G R Grotendorst
    Department of Cell Biology and Anatomy, University of Miami School of Medicine, FL 33136, USA
    Cytokine Growth Factor Rev 8:171-9. 1997
    ..Agents that inhibit CTGF production or action could lead to the development of new therapeutic approaches for the control of fibrotic disorders in humans...
  26. ncbi Growth and differentiation proceeds normally in cells deficient in the immediate early gene NGFI-A
    S L Lee
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 270:9971-7. 1995
    ....
  27. pmc The serum-inducible mouse gene Krox-24 encodes a sequence-specific transcriptional activator
    P Lemaire
    Laboratoire de Genetique Moleculaire, Centre National de la Recherche Scientifique D 1302, Ecole Normale Superieure, Paris, France
    Mol Cell Biol 10:3456-67. 1990
    ..Krox-24 is therefore a sequence-specific transcriptional activator. Krox-24-binding sites were found upstream of several serum-inducible genes, raising the possibility that Krox-24 is involved in the regulation of these genes...
  28. ncbi T cell recognition patterns of immunodominant cytomegalovirus antigens in primary and persistent infection
    Naeem Khan
    Division of Immunology, School of Infection and Host Defense, University of Liverpool, Duncan Building, Daulby Street, Liverpool, UK
    J Immunol 178:4455-65. 2007
    ....
  29. pmc Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant
    R F Greaves
    Department of Medicine, Cambridge University Clinical School, Addenbrookes Hospital, United Kingdom
    J Virol 72:366-79. 1998
    ..One rescued virus (CR249) carried a deletion removing the large intron between exons 1 and 2 of the ie1-ie2 locus, revealing that this intron was dispensable for growth in cell culture...
  30. pmc A human cytomegalovirus antagonist of type I IFN-dependent signal transducer and activator of transcription signaling
    Christina Paulus
    Institut fur Medizinische Mikrobiologie und Hygiene, Forschungszentrum, Universitat Regensburg, D 93047 Regensburg, Germany
    Proc Natl Acad Sci U S A 103:3840-5. 2006
    ..This study identifies the CMV IE1-72kDa protein as a viral antagonist of the cellular innate immune response, inhibiting IFN-dependent STAT signaling by means of an unprecedented molecular mechanism...
  31. ncbi Identification of different specificity requirements between SGK1 and PKBalpha
    James T Murray
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    FEBS Lett 579:991-4. 2005
    ..Our results identify a specific substrate for SGK1 and may facilitate detection of additional physiological substrates for this enzyme...
  32. ncbi Role of SGK1 kinase in regulating glucose transport via glucose transporter GLUT4
    Sankarganesh Jeyaraj
    Institute of Physiology I, University of Tubingen, 72076 Tubingen, Germany
    Biochem Biophys Res Commun 356:629-35. 2007
    ..In summary, SGK1 promotes glucose transporter membrane abundance via GLUT4 phosphorylation at Ser274. Thus, SGK1 may contribute to the insulin and GLUT4-dependent regulation of cellular glucose uptake...
  33. pmc The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL
    M R Wills
    Department of Medicine, University of Cambridge Clinical School, United Kingdom
    J Virol 70:7569-79. 1996
    ....
  34. ncbi Microarray analysis reveals glucocorticoid-regulated survival genes that are associated with inhibition of apoptosis in breast epithelial cells
    Wei Wu
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Cancer Res 64:1757-64. 2004
    ..Given the widespread clinical administration of dexamethasone before chemotherapy, understanding GR-induced survival mechanisms is essential for achieving optimal therapeutic responses...
  35. ncbi Evasion of a single-step, chemotherapy-induced senescence in breast cancer cells: implications for treatment response
    Lynne W Elmore
    Department of Pathology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298 230, USA
    Clin Cancer Res 11:2637-43. 2005
    ..Here, we test the hypothesis that evasion of senescence confers resistance to chemotherapeutic drugs and ionizing radiation...
  36. ncbi Restraint of proinflammatory cytokine biosynthesis by mitogen-activated protein kinase phosphatase-1 in lipopolysaccharide-stimulated macrophages
    Peili Chen
    Stress Signaling Unit, Laboratory of Cellular and Molecular Biology, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA
    J Immunol 169:6408-16. 2002
    ..These findings highlight MKP-1 as a critical negative regulator of the macrophage inflammatory response, underscoring its premise as a potential target for developing novel anti-inflammatory drugs...
  37. ncbi PC3 potentiates NGF-induced differentiation and protects neurons from apoptosis
    Giuseppina Corrente
    Istituto di Neurobiologia, Consiglio Nazionale delle Ricerche, Viale Carlo Marx 15, 00156, Rome, Italy
    Neuroreport 13:417-22. 2002
    ..Such effects might be a consequence of the arrest of cell cycle exerted by PC3TIS21/BTG2, or expression of a sensitizing (neurogenic) property of the molecule...
  38. ncbi Inhibition of connective tissue growth factor (CTGF/CCN2) expression decreases the survival and myogenic differentiation of human rhabdomyosarcoma cells
    Stefania Croci
    Cancer Research Section, Department of Experimental Pathology, University of Bologna, Bologna, Italy
    Cancer Res 64:1730-6. 2004
    ..CTGF emerges as a survival and differentiation factor and could be a new therapeutic target in human rhabdomyosarcoma...
  39. ncbi Conditional expression of the mitogen-activated protein kinase (MAPK) phosphatase MKP-1 preferentially inhibits p38 MAPK and stress-activated protein kinase in U937 cells
    C C Franklin
    Department of Medicine, Division of Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 272:16917-23. 1997
    ..These findings suggest that ERK2-mediated induction of MKP-1 may play an important role in preferentially attenuating signaling through the p38 MAPK and SAPK signal transduction pathways...
  40. pmc CYR61, a product of a growth factor-inducible immediate early gene, promotes angiogenesis and tumor growth
    A M Babic
    Department of Molecular Genetics, M C 669, University of Illinois at Chicago College of Medicine, 900 South Ashland Avenue, Chicago, IL 60607 7170, USA
    Proc Natl Acad Sci U S A 95:6355-60. 1998
    ..Taken together, these results identify CYR61 as an angiogenic inducer that can promote tumor growth and vascularization; the results also suggest potential roles for CYR61 in physiologic and pathologic neovascularization...
  41. pmc Tumor progression and oncogene addiction in a PDGF-B-induced model of gliomagenesis
    Filippo Calzolari
    National Institute for Cancer Research IST, IRCCS, Genoa, Italy
    Neoplasia 10:1373-82, following 1382. 2008
    ....
  42. ncbi The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts
    C C Chen
    Department of Molecular Genetics, University of Illinois at Chicago College of Medicine, 900 South Ashland Ave, Chicago, IL 60607 7170, USA
    J Biol Chem 276:47329-37. 2001
    ..We propose a model in which Cyr61 integrates its activities on endothelial cells, fibroblasts, and macrophages to regulate the processes of angiogenesis, inflammation, and matrix remodeling in the context of cutaneous wound healing...
  43. pmc Varicella-zoster virus ORF63 inhibits apoptosis of primary human neurons
    Chantelle Hood
    Centre for Virus Research, Westmead Millennium Institute, and University of Sydney, P O Box 412, Westmead, 2145 NSW, Australia
    J Virol 80:1025-31. 2006
    ..As ORF63 is expressed in neurons during both productive and latent infection, it may play a significant role in viral pathogenesis by promoting neuron survival during primary and reactivated infections...
  44. ncbi Pro-angiogenic activities of CYR61 (CCN1) mediated through integrins alphavbeta3 and alpha6beta1 in human umbilical vein endothelial cells
    Shr Jeng Leu
    Department of Molecular Genetics, University of Illinois College of Medicine, Chicago, Illinois 60607 7170, USA
    J Biol Chem 277:46248-55. 2002
    ....
  45. ncbi Human cytomegalovirus immediate early proteins and cell growth control
    Jonathan P Castillo
    Program in Immunology and Virology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Gene 290:19-34. 2002
    ..Here, we review the specifics of several HCMV IE proteins, two virion components, and their functions in relation to cell growth control...
  46. ncbi High glucose and hyperinsulinemia stimulate connective tissue growth factor expression: a potential mechanism involved in progression to fibrosis in nonalcoholic steatohepatitis
    V Paradis
    Service d Anatomie Pathologique, Hopital de Bicetre, Le Kremlin Bicetre, France
    Hepatology 34:738-44. 2001
    ..In conclusion, this study suggests that hyperglycemia and insulin are key-factors in the progression of fibrosis in patients with NASH through the up-regulation of CTGF...
  47. pmc Cyclin-dependent kinase activity is required at early times for accurate processing and accumulation of the human cytomegalovirus UL122-123 and UL37 immediate-early transcripts and at later times for virus production
    Veronica Sanchez
    Molecular Biology Section, Mail Code 0366, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0366, USA
    J Virol 78:11219-32. 2004
    ..These results underscore the importance of accurate RNA processing and reiterate the significant role of cell cycle-regulatory factors in HCMV infection...
  48. ncbi The Egr-1 transcription factor directly activates PTEN during irradiation-induced signalling
    T Virolle
    The Burnham Institute, Cancer Research Center, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 3:1124-8. 2001
    ..Loss of Egr-1 expression, which often occurs in human cancers, could deregulate the PTEN gene and contribute to the radiation resistance of some cancer cells...
  49. pmc The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable
    Lisa A Johnson
    Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences I, Rm B240, University of California at Irvine, Irvine, CA 92697 4025, USA
    J Virol 83:6335-46. 2009
    ..In contrast, a decrease in TAP/NXF1 levels severely impaired export of ICP27 and poly(A)(+) RNA. We conclude that TAP/NXF1 is essential for ICP27-mediated export of RNA during HSV-1 infection, whereas Aly/REF may be dispensable...
  50. pmc Target structures of the CD8(+)-T-cell response to human cytomegalovirus: the 72-kilodalton major immediate-early protein revisited
    F Kern
    Institut fur Medizinische Immunologie, Medizinische Fakultät der Humboldt Universität zu Berlin Charité, 10098 Berlin, Germany
    J Virol 73:8179-84. 1999
    ..In summary, our results suggest that IE-1 is far more important as a CD8(+)-T-cell target than current opinion suggests...
  51. ncbi Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex
    J P Rouault
    Unité INSERM U453, Centre Leon Berard, 69373 Lyon Cedex 08, France
    J Biol Chem 273:22563-9. 1998
    ..Finally, we found that box B, one of two conserved domains which define the Btg family, plays a functional role, namely that it is essential to the Btg-mCaf1 interaction...
  52. pmc Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase
    T Kobayashi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI WTB complex, Dow Street, University of Dundee, Dundee DD1 5EH, Scotland, U K
    Biochem J 344:189-97. 1999
    ..Like PKB and SGK1, SGK2 and SGK3 preferentially phosphorylate Ser and Thr residues that lie in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr motifs...
  53. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
    ..We propose that the small G-protein kir/Gem, interacting with beta-subunits, regulates Ca2+ channel expression at the cell surface...
  54. pmc Transcriptional regulation of the interleukin-6 gene of human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
    Hongyu Deng
    Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, 90095, USA
    J Virol 76:8252-64. 2002
    ..We propose that the existence of two vIL-6 promoters offers opportunities for differential regulation of vIL-6 gene expression in different tissue types and may account for the variable vIL-6 levels observed in KS, PEL, and MCD...
  55. pmc Herpes simplex virus-infected cell protein 0 blocks the silencing of viral DNA by dissociating histone deacetylases from the CoREST-REST complex
    Haidong Gu
    Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, 910 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 104:17134-9. 2007
    ..We conclude that CoREST(146-482) functionally replaced ICP0 and that, by extension, ICP0 acts to block the silencing of viral DNA by displacing HDAC1/2 from the CoREST-REST complex...
  56. pmc Control of cytomegalovirus lytic gene expression by histone acetylation
    Jane C Murphy
    Department of Medicine, University of Cambridge, Cambridge CB2 2QQ, UK
    EMBO J 21:1112-20. 2002
    ....
  57. pmc Open reading frame 50 protein of Kaposi's sarcoma-associated herpesvirus directly activates the viral PAN and K12 genes by binding to related response elements
    Pey Jium Chang
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut, USA
    J Virol 76:3168-78. 2002
    ..Two KSHV lytic-cycle promoters are activated by a similar mechanism that involves direct recognition of a homologous response element by the DNA binding domain of ORF 50 protein in the context of related cellular proteins...
  58. ncbi Role and interaction of connective tissue growth factor with transforming growth factor-beta in persistent fibrosis: A mouse fibrosis model
    T Mori
    Department of Dermatology, Kanazawa University School of Medicine, Kanazawa, Ishikawa, Japan
    J Cell Physiol 181:153-9. 1999
    ..The animal model on skin fibrosis by exogenous application of growth factors developed in this study may prove useful for future studies on fibrotic disorders...
  59. pmc Phenotype of a herpes simplex virus type 1 mutant that fails to express immediate-early regulatory protein ICP0
    Roger D Everett
    MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 78:1763-74. 2004
    ....
  60. pmc The BTG2 protein is a general activator of mRNA deadenylation
    Fabienne Mauxion
    CNRS, Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, UPR 2167, Gif sur Yvette, France
    EMBO J 27:1039-48. 2008
    ..This may constitute a common function for BTG/Tob family members...
  61. pmc Mouse cytomegalovirus immediate-early protein 1 binds with host cell repressors to relieve suppressive effects on viral transcription and replication during lytic infection
    Qiyi Tang
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Virol 77:1357-67. 2003
    ..The virus can counter this repression by overexpressing IE1 when present in sufficient copy number, thus reducing the availability and effectiveness of these repressors...
  62. ncbi SOCS/CIS protein inhibition of growth hormone-stimulated STAT5 signaling by multiple mechanisms
    P A Ram
    Department of Biology, Division of Cell Biology, Boston University, Boston, Massachusetts 02215, USA
    J Biol Chem 274:35553-61. 1999
    ....
  63. ncbi Tristetraprolin and other CCCH tandem zinc-finger proteins in the regulation of mRNA turnover
    P J Blackshear
    Office of Clinical Research and the Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Biochem Soc Trans 30:945-52. 2002
    ..Together, the TTP family members should be capable of complex regulation of short-lived mRNAs containing this type of AU-rich instability motif...
  64. ncbi Gem: an induced, immediate early protein belonging to the Ras family
    J Maguire
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Science 265:241-4. 1994
    ..Deregulated Gem expression prevented proliferation of normal and transformed 3T3 cells. These results suggest that Gem is a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane...
  65. pmc The herpes simplex virus type 1 latency-associated transcript (LAT) enhancer/rcr is hyperacetylated during latency independently of LAT transcription
    Nicole J Kubat
    Department of Molecular Genetics and Microbiology, Box 100266, University of Florida College of Medicine, Gainesville, FL 32610 0266, USA
    J Virol 78:12508-18. 2004
    ..This result suggests a role for the 5' exon LAT enhancer region as a cis-acting regulator of transcription that maintains a transcriptionally permissive chromatin domain in the HSV-1 latent episome...
  66. pmc B cell translocation gene 2 enhances susceptibility of HeLa cells to doxorubicin-induced oxidative damage
    Young Bin Lim
    Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, Suwon, 443 721, Korea
    J Biol Chem 283:33110-8. 2008
    ..In summary, BTG2/TIS21/PC3 enhances cancer cell death by accumulating H2O2 via imbalance of the antioxidant enzymes in response to chemotherapy...
  67. ncbi Integrated actions of transforming growth factor-beta1 and connective tissue growth factor in renal fibrosis
    W Qi
    Dept of Medicine, Level 3, Wallace Freeborn Professorial Block, Royal North Shore Hospital, St Leonards, New South Wales, Australia 2065, USA
    Am J Physiol Renal Physiol 288:F800-9. 2005
    ..It has further uniquely demonstrated that CTGF requires TGF-beta, signaling through the TbetaRII in both PTCs and CFs, to exert its fibrogenic response in this in vitro model...
  68. ncbi Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3
    Stefano Farioli-Vecchioli
    Institute of Neurobiology and Molecular Medicine, Consiglio Nazionale Ricerche, Via Fosso di Fiorano 64, 00143, Rome, Italy
    FASEB J 21:2215-25. 2007
    ..As a whole, this indicates that PC3 may prevent medulloblastoma development by controlling cell cycle and promoting differentiation of GCPs...
  69. pmc Kruppel-associated box domain-associated protein-1 as a latency regulator for Kaposi's sarcoma-associated herpesvirus and its modulation by the viral protein kinase
    Pei Ching Chang
    Department of Biological Chemistry and Molecular Medicine and University of California Davis Cancer Center, California 95817, USA
    Cancer Res 69:5681-9. 2009
    ..In summary, the cellular transcriptional repressor KAP-1 plays a role in regulating KSHV latency, and viral protein kinase modulates the chromatin remodeling function of this repressor...
  70. pmc Molecular mechanism of BST2/tetherin downregulation by K5/MIR2 of Kaposi's sarcoma-associated herpesvirus
    Mandana Mansouri
    Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA
    J Virol 83:9672-81. 2009
    ..We further demonstrate that KSHV release is decreased in the absence of K5 in a BST2-dependent manner, suggesting that K5 contributes to the evasion of intracellular antiviral defense programs...
  71. pmc Lytic but not latent infection by Kaposi's sarcoma-associated herpesvirus requires host CSL protein, the mediator of Notch signaling
    Yuying Liang
    Howard Hughes Medical Institute and Department of Microbiology, University of California Medical Center, San Francisco, CA 94143 0414, USA
    Proc Natl Acad Sci U S A 100:8490-5. 2003
    ..RBP-J kappa-mediated repression is therefore not essential for establishment of latent infection, but the RTA-mediated redirection of RBP-J kappa activity from repression to activation is critical for lytic viral replication...
  72. pmc Herpes simplex virus type 1 immediate-early protein ICP0 and is isolated RING finger domain act as ubiquitin E3 ligases in vitro
    Chris Boutell
    Medical Research Council Virology Unit, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 76:841-50. 2002
    ..We conclude that ICP0 has the potential to act as an E3 ubiquitin ligase during viral infection and to target specific cellular proteins for destruction by the 26S proteasome...
  73. ncbi mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1)
    Juan M García-Martínez
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 416:375-85. 2008
    ..The results of the present study indicate that NDRG1 phosphorylation represents an excellent biomarker for mTORC2 activity...
  74. pmc Reciprocal activities between herpes simplex virus type 1 regulatory protein ICP0, a ubiquitin E3 ligase, and ubiquitin-specific protease USP7
    Chris Boutell
    MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 79:12342-54. 2005
    ..We propose that the biological significance of the ICP0-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP0 are expressed...
  75. pmc Pathways of Egr-1-mediated gene transcription in vascular biology
    E S Silverman
    Pulmonary and Critical Care Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Am J Pathol 154:665-70. 1999
  76. ncbi The low density lipoprotein receptor-related protein/alpha2-macroglobulin receptor is a receptor for connective tissue growth factor
    P R Segarini
    FibroGen, Inc, South San Francisco, California 94080, USA
    J Biol Chem 276:40659-67. 2001
    ..Last, CTGF is rapidly internalized and degraded and this process is LRP-dependent. In summary, our data indicate that LRP is a receptor for CTGF, and may play an important role in mediating CTGF biology...
  77. ncbi Connective tissue growth factor and its regulation: a new element in diabetic glomerulosclerosis
    B L Riser
    Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan 48202, USA
    Ren Fail 23:459-70. 2001
    ..These results suggest that CTGF upregulation is an important factor in the pathogenesis of mesangial matrix accumulation in both diabetic and non-diabetic glomerulosclerosis, acting downstream of TGF-beta...
  78. ncbi Depolarization strongly induces human cytomegalovirus major immediate-early promoter/enhancer activity in neurons
    D G Wheeler
    Department of Physiology, McGill University, , , Canada
    J Biol Chem 276:31978-85. 2001
    ..These results may provide insight into molecular mechanisms of cytomegalovirus-related diseases of the brain...
  79. ncbi Enhancement of transactivation activity of Rta of Epstein-Barr virus by RanBPM
    Li Kwan Chang
    Institute of Microbiology and Biochemistry, Department of Biochemical Science and Technology, National Taiwan University, No 1, Sec 4, Roosevelt Rd, Taipei, 10617, Taiwan
    J Mol Biol 379:231-42. 2008
    ..This fact explains why the expression of RanBPM enhances the transactivation activity of Rta. Taken together, the present results indicate a new role of RanBPM in regulating a viral protein that is critical to EBV lytic activation...
  80. ncbi Novel growth factors involved in the pathogenesis of proliferative vitreoretinopathy
    D R Hinton
    Department of Ophthalmology Keck School of Medicine of the University of Southern California Los Angeles, CA 90033, USA
    Eye (Lond) 16:422-8. 2002
    ..Methods Immunohistochemical methods (ABC Elite) were used to demonstrate the presence of HGF and CTGF in cryostat sections of five human PVR membranes...
  81. ncbi Genes modulated by expression of GD3 synthase in Chinese hamster ovary cells. Evidence that the Tis21 gene is involved in the induction of GD3 9-O-acetylation
    Honoo Satake
    Glycobiology Research and Training Center, Department of Medicine, University of California, San Diego, La Jolla 92093 0687, USA
    J Biol Chem 278:7942-8. 2003
    ..This indicates separate machineries for 9-O-acetylation on alpha2-8-linked sialic acids of gangliosides and on alpha2-6-linked sialic acids on N-glycans...
  82. ncbi Post-translational modification of Rta of Epstein-Barr virus by SUMO-1
    Li Kwan Chang
    Faculty of Biological Medicine and Environmental Biology and Graduate Institute of Biochemistry, Kaohsiung Medical University, 100, Shih Chuan 1st Road, Kaohsiung, Taiwan
    J Biol Chem 279:38803-12. 2004
    ..These results indicate that sumoylation of Rta may be important in EBV lytic activation...
  83. ncbi Connexin43 interacts with NOV: a possible mechanism for negative regulation of cell growth in choriocarcinoma cells
    Alexandra Gellhaus
    Institute of Anatomy and Cell Biology, University Hospital Essen, 55 Hufelandstrasse, 45122 Essen, Germany
    J Biol Chem 279:36931-42. 2004
    ..We suggest that Cx43 is able to regulate cell growth via an up-regulation of NOV transcription, a change in localization of the NOV protein and a binding of NOV to the C terminus of Cx43...
  84. pmc Spontaneous molecular reactivation of herpes simplex virus type 1 latency in mice
    Lawrence T Feldman
    Department of Microbiology and Immunology, University of California Medical Center, Los Angeles, CA 90024, USA
    Proc Natl Acad Sci U S A 99:978-83. 2002
    ..In the absence of detectable infectious virus in these ganglia, we propose the term "spontaneous molecular reactivation" to describe this ongoing process...
  85. pmc Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells
    P Lemaire
    Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 85:4691-5. 1988
    ..These properties suggest that Krox-20 and Krox-24 may encode transcription factors with identical DNA target sequences and that these factors may be involved in the modulation of cell proliferation and differentiation...
  86. pmc Herpes simplex virus ICP0 and ICP34.5 counteract distinct interferon-induced barriers to virus replication
    Karen L Mossman
    Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Virol 76:1995-8. 2002
    ..5...
  87. ncbi TIS21/BTG2/PC3 is expressed through PKC-delta pathway and inhibits binding of cyclin B1-Cdc2 and its activity, independent of p53 expression
    Min Sook Ryu
    Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, Suwon 443 721, South Korea
    Exp Cell Res 299:159-70. 2004
    ..In summary, TPA-induced TIS21 mRNA expression is mediated by PKC-delta, and TIS21 induces G2/M arrest and cell death by inhibiting cyclin B1-Cdc2 binding and the kinase activity through its binding to Cdc2...
  88. ncbi The role of early growth response gene 1 (egr-1) in regulation of the immune response
    S B McMahon
    Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia, USA
    J Leukoc Biol 60:159-66. 1996
    ..The role of egr-1 in regulation of the immune response will be discussed in the context of these recent studies...
  89. pmc Cell cycle arrest by human cytomegalovirus 86-kDa IE2 protein resembles premature senescence
    Emanuela Noris
    Department of Public Health and Microbiology, University of Turin, Italy
    J Virol 76:12135-48. 2002
    ..Altogether our results demonstrate for the first time that HCMV, after arresting the cell cycle and inhibiting apoptosis, triggers the cellular senescence program, probably through the p16(INK4a) and p53 pathways...
  90. pmc Autorepression of the human cytomegalovirus major immediate-early promoter/enhancer at late times of infection is mediated by the recruitment of chromatin remodeling enzymes by IE86
    Matthew Reeves
    Department of Medicine, Box 157, Level 5, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom
    J Virol 80:9998-10009. 2006
    ....
  91. pmc Multiple endocytic trafficking pathways of MHC class I molecules induced by a Herpesvirus protein
    Robert E Means
    Department of Microbiology and Molecular Genetics, Division of Tumor Virology, New England Regional Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01772, USA
    EMBO J 21:1638-49. 2002
    ..These results demonstrate a novel trafficking mechanism of MHC class I molecules induced by KSHV K3, which ensures viral escape from host immune effector recognition...
  92. ncbi Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ICP0
    P Lomonte
    Medical Research Council Virology Unit, Glasgow G11 5JR, Scotland, United Kingdom
    J Biol Chem 276:5829-35. 2001
    ....
  93. pmc Comparative study of regulation of RTA-responsive genes in Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8
    Moon Jung Song
    Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, Jonsson Comprehensive Cancer Center, and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 77:9451-62. 2003
    ..Based on these results, we conclude that direct binding of RTA to these target sequences contributes to their gene expression to various extents during the lytic life cycle of KSHV...
  94. ncbi A family of cytokine-inducible inhibitors of signalling
    R Starr
    The Walter and Eliza Hall Institute for Medical Research, Parkville, Victoria, Australia
    Nature 387:917-21. 1997
    ..Transcription of all four SOCS genes is increased rapidly in response to interleukin-6, in vitro and in vivo, suggesting they may act in a classic negative feedback loop to regulate cytokine signal transduction...
  95. ncbi Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0
    Patrick Lomonte
    Viral Silencing and Centromeric Instability Team, Universite de Lyon, Lyon, F 69003, France
    FEBS Lett 581:658-62. 2007
    ..CENP-B is one of the major proteins of centromeres and its degradation is likely to contribute to the severe damage induced to centromeres by ICP0...
  96. pmc Inhibition of macroautophagy triggers apoptosis
    Patricia Boya
    CNRS UMR 8125, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Mol Cell Biol 25:1025-40. 2005
    ..All together, these data indicate that autophagy may be cytoprotective, at least under conditions of nutrient depletion, and point to an important cross talk between type 1 and type 2 cell death pathways...
  97. pmc Dynamic regulation of pro- and anti-inflammatory cytokines by MAPK phosphatase 1 (MKP-1) in innate immune responses
    Hongbo Chi
    Section of Immunobiology, Department of Pharmacology, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 103:2274-9. 2006
    ..These results highlight the complex mechanisms by which the MAPKs regulate innate immunity...
  98. pmc Replication of ICP0-null mutant herpes simplex virus type 1 is restricted by both PML and Sp100
    Roger D Everett
    MRC Virology Unit, Church Street, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 82:2661-72. 2008
    ..These data lend further weight to the hypothesis that ND10 play an important role in the regulation of HSV-1 gene expression...
  99. pmc Quantitative membrane proteomics reveals new cellular targets of viral immune modulators
    Eric Bartee
    Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America
    PLoS Pathog 2:e107. 2006
    ..The unbiased global proteome analysis therefore revealed novel immunomodulatory functions of pathogen proteins...
  100. pmc Role of EGR1 in regulation of stimulus-dependent CD44 transcription in B lymphocytes
    J S Maltzman
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, 19104, USA
    Mol Cell Biol 16:2283-94. 1996
    ..These studies identify EGR1 as an intermediary linking BCR-derived signals to the induction of CD44. The relevance of these molecular events to BCR signal transduction and antigen-stimulated B-cell-mediated immune responses is discussed...
  101. ncbi A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency
    G A Taylor
    Howard Hughes Medical Institute Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA
    Immunity 4:445-54. 1996
    ..TTP-deficient mice may serve as useful models of the autoimmune inflammatory state resulting from chronic effective TNF alpha excess...

Research Grants63

  1. Alteration of ER:mitochondrial contacts by human cytomegalovirus infection
    ANAMARIS MARTHA COLBERG POLEY; Fiscal Year: 2010
    ..Trafficking of its UL37 immediate early proteins from the endoplasmic reticulum (ER) to mitochondria is unconventional but central to its replication and ..
  2. Epigenetic determinants of Epstein-Barr virus and cellular DNA in oral diseases
    SHANNON CELESTE KENNEY; Fiscal Year: 2013
    ..The proposed studies should greatly enhance our understanding of how EBV normally replicates in differentiated epithelial cells yet achieves long term viral latency in undifferentiated epithelial tumor cells. ..
  3. EARLY INTERACTIONS OF DNA VIRUSES AND HOST
    Gerd Maul; Fiscal Year: 2009
    ..Whether a mouse model inducibly expressing the immediate early proteins can elucidate the early steps in the multi-step reactivation sequence and cell-specific expression of IE1 ..
  4. Are the suppressors of cytokine signaling involved in Alzheimer's disease?
    DOUGLAS GORDON WALKER; Fiscal Year: 2011
    ..SOCS are immediate early proteins with short half-lives;their presence in tissue indicates ongoing responses to pathology...
  5. Inhibition of mTOR Alters Epstein-Barr Virus Lytic Replication
    Amy L Adamson; Fiscal Year: 2012
    ..specific aims of this proposal are #1) to determine how the mTOR pathway alters the functions of the EBV immediate early proteins BZLF1 and BRLF1, and whether such alterations are cell-type specific, and #2) to determine whether EBV ..
  6. INHIBITING TRANSACTIVATION OF HIV-1
    Robert Ricciardi; Fiscal Year: 1992
    ..Different DNA elements of the HIV LTR may be involved in transactivation by these immediate early proteins and tat...
  7. Silencing Suppression Mechanism of a Herpes Simplex Virus Virion Component
    NIL CELEBI CHERUKURI; Fiscal Year: 2009
    ....
  8. Modification of RNA polymerase II by HSV-1 proteins
    Stephen Rice; Fiscal Year: 2006
    ..This research will also help to elucidate the fundamental replication mechanisms of HSV-1 and other pathogenic herpesviruses. ..
  9. MATURATION OF HERPESVIRAL NUCLEAR PROTEINS
    David Knipe; Fiscal Year: 2003
    The long-term goals of this research are to define the mechanisms by which the herpes simplex virus immediate early proteins move into the infected cell nucleus and regulate viral gene expression...
  10. HIV-HSV INTERACTIONS IN VITRO
    MARY NIES; Fiscal Year: 1993
    ..These studies should more clearly define the mechanisms by which HSV upregulates HIV expression and may indicate whether HSV may serve as a permissive cofactor for HIV replication under certain conditions...
  11. REGULATION OF CELLULAR FUNCTIONS BY DNA TUMOR VIRUSES
    JAMES ALWINE; Fiscal Year: 2009
    ..SV40 T antigen and HCMV major immediate early proteins (MIEPs) activate Akt kinase through activation of phosphatidylinositide 3'-OH kinase (PI3K)...
  12. HORMONES, IMMEDIATE EARLY PROTEINS AND BEHAVIOR
    JEFFREY BLAUSTEIN; Fiscal Year: 2000
    ....
  13. REGULATION OF HERPES PROTEINS IN PERSISTENT INFECTION
    Gretchen Caughman; Fiscal Year: 1993
    ..Specifically, the aims are to: 1) characterize the structure and function of the EHV-1 immediate early proteins (IEPs) and define further the alterations in their regulation, synthesis, and localization; 2) determine ..
  14. CYTOMEGALOVIRUS AND MHC CLASS II IN TRANSPLANTATION
    Daniel Sedmak; Fiscal Year: 1999
    ..Based upon preliminary data, the investigators hypothesize that CMV immediate early proteins play a major role in the inhibition of class II transcription, which will be investigated in Specific Aim ..
  15. CYTOMEGALOVIRUS IMMEDIATE EARLY PROTEINS AND VIRION RNAS
    Thomas E Shenk; Fiscal Year: 2010
    ..HCMV replication will be studied in fibroblasts, the cell commonly used to propagate HCMV;endothelial cells and epithelial cells. ..
  16. Human Cytomegalovirus and the Unfolded Protein Response
    JENNIFER ISLER; Fiscal Year: 2004
    ..Our data, and the data of others, suggest that HCMV immediate early proteins target critical cellular regulators and alter the processes they control to benefit viral infection...
  17. Disruption of the SGk1 gene in aldosterone target cells.
    ANIKO NARAY FEJES TOTH; Fiscal Year: 2002
    ..abstract_text> ..
  18. ALDO SPECIFICITY OF MINERALOCORTICOID TARGET CELLS
    ANIKO NARAY FEJES TOTH; Fiscal Year: 2009
    ..WT mice, we will identify aldosterone-regulated genes that are not downstream of SGK1. CCD cells will be isolated by immuno-selection, and differentially expressed genes identified using DNA microarrays. ..
  19. REGULATION AND FUNCTION OF THE ALPHA SUBUNIT OF ENAC
    Christie Thomas; Fiscal Year: 2003
    ..The long-term objectives are to understand the regulation and function of ENaC in transepithelial Na+ transport. ..
  20. Egr-1, PKC Beta, Signalling and Atherosclerosis
    Shi Fang Yan; Fiscal Year: 2009
    ....
  21. Early Growth Response-1 (Egr-1), PKC Beta and Restenosis
    Shi Fang Yan; Fiscal Year: 2007
    ..abstract_text> ..
  22. Sgk1 in Na+ transport in fetal lung epithelia
    Christie Thomas; Fiscal Year: 2007
    ..abstract_text> ..
  23. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2001
    ....
  24. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2008
    ..abstract_text> ..
  25. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2002
    ..The experimental design includes identification of the products of alternative splicing and characterization of the role of the ligands in infection. ..
  26. FUNCTIONS FOR HERPES SIMPLEX VIRUS ICP22 AND US1.5
    Bernard Roizman; Fiscal Year: 2009
    ..In all instances a key question to be resolved is the contribution of each of these functions of ICP22 to the biology of the virus in the experimental animal host. ..
  27. HIV Epitope Specific T Cell Responses and Control of HIV Replication
    Mark Jacobson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  28. Genes That Regulate Neuronal Cell Death
    Robert Freeman; Fiscal Year: 2008
    ..They will also provide new information concerning the function of EGLN-catalyzed proline hydroxylation as a novel mechanism for altering protein function in neurons. ..
  29. Hyperoxaluria and Nephrolithiasis After Gastric Bypass Surgery for Obesity
    Rajiv Kumar; Fiscal Year: 2008
    ..Finally, we will study why these complications occur and we will develop ways in which to prevent kidney stones and loss of kidney function after gastric bypass surgery. [unreadable] [unreadable] [unreadable]..
  30. Aberrant T Cell Function and Immunopathogenesis of CMV Immune Recovery Uveitis
    Mark Jacobson; Fiscal Year: 2008
    ..Thus, understanding the disease mechanism of CMV IRU could facilitate development of effective therapy for CMV IRU or diagnostic or treatment strategies to prevent CMV IRU. [unreadable] [unreadable] [unreadable]..
  31. CYR61-Mediated Angiogenesis in Rheumatoid Arthritis
    LESTER LAU; Fiscal Year: 2002
    ..Then we will explore Cyr61 as a potential therapeutic target. We will test whether blockade of Cyr6l function, using monoclonal antibodies or inhibitory peptides, can prevent or ameliorate arthritis and joint destruction. ..
  32. Mechanisms of Resistance to Radio Inducible Gene Therapy
    Ralph Weichselbaum; Fiscal Year: 2009
    ..Egr-TNF +IR by enhancing resistance of the tumor endothelium. Specific Aim 3. Explore the role of tumor cell resistance to Ad.Egr-TNF+IR. ..
  33. Regulation of Fibrogenesis by the Dietary Flavonoids
    Dennis Ricupero; Fiscal Year: 2002
    ....
  34. Tissue Factor-factor Vlla signaling
    Usha Pendurthi; Fiscal Year: 2010
    ..The proposed studies will provide valuable information in understanding how TF contributes to tumorigenesis. These studies will also provide clues on potential therapeutic targets for prevention of cancer progression. ..
  35. FoxM1B Protein in Stimulating Proliferation during Aging
    LESTER LAU; Fiscal Year: 2007
    ..abstract_text> ..
  36. REGULATIOIN OF RENAL PHOSPHATE EXCRETION AND VITAMIN D METABOLISM BY FGF 7
    Rajiv Kumar; Fiscal Year: 2010
    ..The interaction between the FGF 7 and other PTNs will be clarified. Such information will significantly enhance our knowledge of mineralization processes and Pi homeostasis. ..
  37. DNA Damage Targeted Gene Therapy in Head & Neck Cancer
    Ralph Weichselbaum; Fiscal Year: 2009
    ..MRI measurements will be performed to detect changes in blood flow and/or vascular destruction and cell death following treatment with ad.Egr-TNF.11D and XRT. These studies will lead to new therapies and future clinical trials. ..
  38. Structure and Function of the Matricellular Protein CCN1
    LESTER LAU; Fiscal Year: 2008
    ..Through these studies, we hope to gain insight into how CCN1 acts as a matricellular protein to control multiple cellular processes. ..
  39. Integrin-Matrix Interaction in Cardiovascular Development
    Lester F Lau; Fiscal Year: 2010
    ..Together, these studies will provide novel insights into how CCN proteins regulate cardiovascular development through extracellular matrix signaling, and shed light on their roles in cardiovascular diseases. ..
  40. THE STRUCTURE OF CALBINDIN-D28K
    Rajiv Kumar; Fiscal Year: 2004
    ..These findings will potentially be of significance in the understanding of neurodegenerative disorders, cerebellar function and long-term hippocampal potentiation. ..
  41. Mechanisms of Neurotrophin dependent Survival
    Robert Freeman; Fiscal Year: 2005
    ..Together these experiments will test the importance of NF-kB for neurotrophinregulated survival and they will uncover important new information concerning the mechanisms by which neurotrophins protect neurons from cell death. ..
  42. REGULATION OF HUMAN CYTOMEGALOVIRUS GENE EXPRESSION
    Mark Stinski; Fiscal Year: 2005
    ..We will investigate the functions of this replication defective HCMV in activation of viral or cellular gene expression and compare these functions to those of the 1E72 and 1E86 proteins. ..
  43. CMV Immune Response & Long-Term Outcome of CMV Retinitis
    Mark Jacobson; Fiscal Year: 2005
    ....
  44. Gene Therapy for Extension of Vein Graft Patency
    Ralph Weichselbaum; Fiscal Year: 2005
    ..abstract_text> ..
  45. Ribosome Biogenesis and p53 Dependent Cell Cycle Control
    LESTER LAU; Fiscal Year: 2006
    ..Through these studies we hope to elucidate the mechanism by which nucleolar surveillance functions as a novel cell cycle checkpoint. ..
  46. Adjunct rhIL-12 enhance THI response to viral vaccine?
    Mark Jacobson; Fiscal Year: 2004
    ..g. CMV, HSV and HIV). ..
  47. INDUCIBLE GENES IN ANGIOGENESIS AND TUMOR GROWTH
    LESTER LAU; Fiscal Year: 2003
    ..Third, the role of Cyr61 in tumor growth and metastasis will be evaluated, and intervention of Cyr61 activities will be explored as a means of restricting tumor growth. ..
  48. Connective Tissue Growth Factor in Hepatic Fibrosis
    David Brigstock; Fiscal Year: 2006
    ..In addition, these studies will help establish whether CTGF is a therapeutic target for treating fibrosis, which is a contributing factor in 45 percent of deaths in the USA. ..
  49. Multidisciplinary Training in Developmental Neuroscience
    Robert Freeman; Fiscal Year: 2007
    ..A plan is also in place that will increase the pool of predoctoral applicants from underrepresented racial and ethnic groups. [unreadable] [unreadable]..
  50. Modulation of K channels in renal collecting duct
    Wen Hui Wang; Fiscal Year: 2007
    ....
  51. THE PHYSIOLOGY AND METABOLISM OF VITAMIN D
    Rajiv Kumar; Fiscal Year: 2003
    ..Results of this investigation could lead to a better understanding of the pathogenesis of bone diseases and the formulation of new therapeutic strategies in disorders such as hyperparathyroidism and osteoporosis. ..
  52. EXPRESSION AND STRUCTURE OF THE CALCIUM SENSING RECEPTOR
    Rajiv Kumar; Fiscal Year: 2007
    ..Furthermore, this information will serve as the basis for more extensive experiments involving the structure of the full-length receptor. [unreadable] [unreadable]..
  53. Biology of a Novel Phosphaturic Protein - sFRP-4
    Rajiv Kumar; Fiscal Year: 2007
    ..Our findings will have important implications with respect to Pi homeostasis, bone mineralization in mammals and humans, and the regulation of ion transport through Wnt signaling in the kidney. ..
  54. The Lateral Septum, Urocortin and Alcohol
    ADAM WEITEMIER; Fiscal Year: 2004
    ..Findings of this proposal will provide an important contribution to work highlighting the importance of stress- and anxiety-modulating neuropeptide systems in the regulation of alcohol-related behaviors. ..
  55. CASPASE 8 AND ITS HOMOLOGS IN NF-KB AND JNK ACTIVATION
    PREET CHAUDHARY; Fiscal Year: 2004
    ..abstract_text> ..
  56. Role of VEGF in Neonatal Pulmonary Hypertension
    Theresa Grover; Fiscal Year: 2008
    ..These studies will provide an integrated approach to the hypothesis, incorporating whole animal physiology coupled with molecular and cellular studies. ..
  57. Somatomedin: Autologous Regulator of Bone Formation
    Ernesto Canalis; Fiscal Year: 2008
    ..These investigations should clarify the role of CHOP in bone cell differentiation and function. [unreadable] [unreadable]..
  58. NO-MEDIATED MODIFICATION OF NMDA RECEPTOR DURING HYPOXIA
    Om Mishra; Fiscal Year: 2008
    ..abstract_text> ..
  59. Post-transcriptional regulation of TNF-alpha production
    Paul Anderson; Fiscal Year: 2005
    ..These studies might also identify molecular targets for the development of orally available TNFa blockers that can be used to treat patients with rheumatoid arthritis. ..
  60. Chemical Complementation Assay for MKP-1 (RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..This proposal will provide sufficient reagents, including cells and DMA transfection vectors, to permit the high throughput screening of 100,000 compounds. ..
  61. Genes in X-linked Ectodermal Dysplasia Receptor
    PREET CHAUDHARY; Fiscal Year: 2006
    ..In the long-term these studies may lead to better diagnosis and treatment of ectodermal dysplasias and craniofacial abnormalities. ..