myoglobin

Summary

Summary: A conjugated protein which is the oxygen-transporting pigment of muscle. It is made up of one globin polypeptide chain and one heme group.

Top Publications

  1. ncbi Evolution of myoglobin
    T Suzuki
    Laboratory of Biochemistry, Faculty of Science, Kochi University, Japan
    Cell Mol Life Sci 54:979-1004. 1998
  2. pmc Nitrite regulates hypoxic vasodilation via myoglobin-dependent nitric oxide generation
    Matthias Totzeck
    Department of Medicine, Division of Cardiology, Pulmonology, and Vascular Medicine, University Hospital Duesseldorf, Germany
    Circulation 126:325-34. 2012
  3. pmc Myoglobin forms amyloid fibrils by association of unfolded polypeptide segments
    Marcus Fändrich
    Institut fur Molekulare Biotechnologie, Beutenbergstrasse 11, D 07745 Jena, Germany
    Proc Natl Acad Sci U S A 100:15463-8. 2003
  4. pmc Imaging the migration pathways for O2, CO, NO, and Xe inside myoglobin
    Jordi Cohen
    Department of Physics and Beckman Institute, University of Illinois, Urbana, Illinois, USA
    Biophys J 91:1844-57. 2006
  5. ncbi Mapping the network of pathways of CO diffusion in myoglobin
    Luca Maragliano
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA
    J Am Chem Soc 132:1010-7. 2010
  6. ncbi Characteristics and mechanism of formation of peroxide-induced heme to protein cross-linking in myoglobin
    Brandon J Reeder
    Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Biochemistry 41:367-75. 2002
  7. ncbi Watching a protein as it functions with 150-ps time-resolved x-ray crystallography
    Friedrich Schotte
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:1944-7. 2003
  8. pmc Nitrite reductase activity of myoglobin regulates respiration and cellular viability in myocardial ischemia-reperfusion injury
    Ulrike B Hendgen-Cotta
    Department of Medicine, Division of Cardiology, Pulmonology and Vascular Medicine, University Hospital Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany
    Proc Natl Acad Sci U S A 105:10256-61. 2008
  9. ncbi Myoglobin function reassessed
    Jonathan B Wittenberg
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Exp Biol 206:2011-20. 2003
  10. pmc Protein collective motions coupled to ligand migration in myoglobin
    Yasutaka Nishihara
    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto Japan
    Biophys J 98:1649-57. 2010

Detail Information

Publications355 found, 100 shown here

  1. ncbi Evolution of myoglobin
    T Suzuki
    Laboratory of Biochemistry, Faculty of Science, Kochi University, Japan
    Cell Mol Life Sci 54:979-1004. 1998
    The distribution, physiological function, amino acid sequence and gene structure of myoglobin and myoglobin-like proteins from various taxa are summarized, and their evolution is discussed...
  2. pmc Nitrite regulates hypoxic vasodilation via myoglobin-dependent nitric oxide generation
    Matthias Totzeck
    Department of Medicine, Division of Cardiology, Pulmonology, and Vascular Medicine, University Hospital Duesseldorf, Germany
    Circulation 126:325-34. 2012
    ..We have shown that deoxygenated myoglobin in the heart can reduce nitrite to nitric oxide (NO·) and thereby contribute to cardiomyocyte NO· signaling ..
  3. pmc Myoglobin forms amyloid fibrils by association of unfolded polypeptide segments
    Marcus Fändrich
    Institut fur Molekulare Biotechnologie, Beutenbergstrasse 11, D 07745 Jena, Germany
    Proc Natl Acad Sci U S A 100:15463-8. 2003
    ..However, it is inevitable that such conditions often stabilize protein folding intermediates...
  4. pmc Imaging the migration pathways for O2, CO, NO, and Xe inside myoglobin
    Jordi Cohen
    Department of Physics and Beckman Institute, University of Illinois, Urbana, Illinois, USA
    Biophys J 91:1844-57. 2006
    b>Myoglobin (Mb) is perhaps the most studied protein, experimentally and theoretically. Despite the wealth of known details regarding the gas migration processes inside Mb, there exists no fully conclusive picture of these pathways...
  5. ncbi Mapping the network of pathways of CO diffusion in myoglobin
    Luca Maragliano
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA
    J Am Chem Soc 132:1010-7. 2010
    The pathways of diffusion of a CO molecule inside a myoglobin protein and toward the solvent are investigated...
  6. ncbi Characteristics and mechanism of formation of peroxide-induced heme to protein cross-linking in myoglobin
    Brandon J Reeder
    Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Biochemistry 41:367-75. 2002
    At acidic pH values heme-protein cross-linked myoglobin (Mb-H) forms as a product of a peroxide-induced ferric-ferryl redox cycle...
  7. ncbi Watching a protein as it functions with 150-ps time-resolved x-ray crystallography
    Friedrich Schotte
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:1944-7. 2003
    We report picosecond time-resolved x-ray diffraction from the myoglobin (Mb) mutant in which Leu29 is replaced by Phe (L29Fmutant). The frame-by-frame structural evolution, resolved to 1...
  8. pmc Nitrite reductase activity of myoglobin regulates respiration and cellular viability in myocardial ischemia-reperfusion injury
    Ulrike B Hendgen-Cotta
    Department of Medicine, Division of Cardiology, Pulmonology and Vascular Medicine, University Hospital Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany
    Proc Natl Acad Sci U S A 105:10256-61. 2008
    ..Studies suggest that hemoglobin and myoglobin may subserve a fundamental physiological function as hypoxia dependent nitrite reductases...
  9. ncbi Myoglobin function reassessed
    Jonathan B Wittenberg
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Exp Biol 206:2011-20. 2003
    ..Within the sarcoplasm, myoglobin, a mobile carrier of oxygen, is developed in response to mitochondrial demand and augments the flow of oxygen to ..
  10. pmc Protein collective motions coupled to ligand migration in myoglobin
    Yasutaka Nishihara
    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto Japan
    Biophys J 98:1649-57. 2010
    Ligand migration processes inside myoglobin and protein dynamics coupled to the migration were theoretically investigated with molecular dynamics simulations...
  11. ncbi Myoglobin-deficient mice activate a distinct cardiac gene expression program in response to isoproterenol-induced hypertrophy
    Andrei Molojavyi
    Institut für Herz und Kreislaufphysiologie, Heinrich Heine Universitat Dusseldorf, 40001 Dusseldorf, Germany
    Physiol Genomics 41:137-45. 2010
    b>Myoglobin knockout mice (myo-/-) adapt to the loss of myoglobin by the activation of a variety of compensatory mechanisms acting on the structural and functional level...
  12. ncbi Intra- and intermolecular beta-pleated sheet formation in glutamine-repeat inserted myoglobin as a model for polyglutamine diseases
    M Tanaka
    Laboratory for CAG Repeat Diseases, RIKEN Brain Science Institute, Saitama 351-0198, Japan
    J Biol Chem 276:45470-5. 2001
    ..To elucidate structural properties of the expanded polyglutamine, we prepared sperm whale myoglobin (Mb) mutants, in which 12, 28, 35, and 50 repeats of glutamine were inserted at the corner between the C and D ..
  13. ncbi Utility of urine myoglobin for the prediction of acute renal failure in patients with suspected rhabdomyolysis: a systematic review
    Karina Rodriguez-Capote
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    Clin Chem 55:2190-7. 2009
    Urine myoglobin continues to be used as a marker of rhabdomyolysis, particularly to assess risk of developing acute renal failure and evaluate treatment success...
  14. ncbi Body size and skeletal muscle myoglobin of cetaceans: adaptations for maximizing dive duration
    S R Noren
    Department of Biology, Earth and Marine Sciences Building, University of California, Santa Cruz 95064, USA
    Comp Biochem Physiol A Mol Integr Physiol 126:181-91. 2000
    ..Furthermore, myoglobin content is a key oxygen store in the muscle as it is many times higher in marine mammals than terrestrial ..
  15. ncbi Thyroid hormone stimulates myoglobin gene expression in rat cardiac muscle
    Gisele Giannocco
    Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508 900 Sao Paulo, SP, Brazil
    Mol Cell Endocrinol 226:19-26. 2004
    T3 increases the heart activity, O2 consumption and the reactive O2 species production. Myoglobin (Mb) is highly expressed in the heart, where it facilitates O2 diffusion, mitochondrial respiration, and scavenges reactive O2 species...
  16. ncbi Old proteins - new locations: myoglobin, haemoglobin, neuroglobin and cytoglobin in solid tumours and cancer cells
    T A Gorr
    Institute of Veterinary Physiology, University of Zurich, Zurich, Switzerland
    Acta Physiol (Oxf) 202:563-81. 2011
    The unexpected identification of myoglobin (MB) in breast cancer prompted us to evaluate the clinico-pathological value of MB, haemoglobin (HB) and cytoglobin (CYGB) in human breast carcinoma cases...
  17. ncbi Functional differentiation of myoglobin isoforms in hypoxia-tolerant carp indicates tissue-specific protective roles
    Signe Helbo
    Department of Bioscience, Aarhus University, Denmark
    Am J Physiol Regul Integr Comp Physiol 302:R693-701. 2012
    ..genome duplication, the hypoxia-tolerant common carp and goldfish are the only vertebrates known to possess two myoglobin (Mb) paralogs...
  18. ncbi Toward quantitative simulations of carbon monoxide escape pathways in myoglobin
    Ron Elber
    Department of Chemistry and Biochemistry, and Institute of Computational Sciences and Engineering, University of Texas at Austin, 1 University Station, ICES, C0200, Austin, Texas 78712, USA
    J Phys Chem B 112:6147-54. 2008
    ..molecular dynamics trajectories have been computed to explore the diffusion of carbon monoxide through myoglobin. The classical equations of motion were integrated for 2 ns and the resulting pathways analyzed...
  19. pmc Regulation of myoglobin expression
    Shane B Kanatous
    Department of Biology, Colorado State University, Fort Collins, CO 80523, USA
    J Exp Biol 213:2741-7. 2010
    b>Myoglobin is a well-characterized, cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers...
  20. ncbi Non-thermal effects of electromagnetic fields at mobile phone frequency on the refolding of an intracellular protein: myoglobin
    Fabrizio Mancinelli
    Dipartimento di Biochimica e Biofisica, Seconda Universita degli Studi di Napoli, Italy
    J Cell Biochem 93:188-96. 2004
    ..have been observed on the refolding kinetics of the heme binding site in an intracellular protein: tuna myoglobin, starting from acidic conditions...
  21. pmc Differential loss and retention of cytoglobin, myoglobin, and globin-E during the radiation of vertebrates
    Federico G Hoffmann
    School of Biological Sciences, University of Nebraska, Lincoln, USA
    Genome Biol Evol 3:588-600. 2011
    ..overwhelming majority of jawed vertebrates (gnathostomes) possess copies of the related cytoglobin (Cygb) and myoglobin (Mb) genes...
  22. ncbi Nature of the Fe-O2 bonding in oxy-myoglobin: effect of the protein
    Hui Chen
    Department of Organic Chemistry and the Lise Meitner Minerva Center for Computational Quantum Chemistry, The Hebrew University of Jerusalem, Givat Ram Campus, 91904 Jerusalem, Israel
    J Am Chem Soc 130:14778-90. 2008
    The nature of the Fe-O2 bonding in oxy-myoglobin was probed by theoretical calculations: (a) QM/MM (hybrid quantum mechanical/molecular mechanical) calculations using DFT/MM and CASSCF/MM methods and (b) gas-phase calculations using DFT (..
  23. ncbi Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: potential role in cardioprotection
    Nicole L Lohr
    Department of Anesthesiology, Medical College of Wisconsin, MEB 4245, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    J Mol Cell Cardiol 47:256-63. 2009
    ..Thus, the photodissociation to NO and its synergistic effect with sodium nitrite may represent a noninvasive and site-specific means for increasing NO bioavailability...
  24. ncbi Convective oxygen transport and tissue oxygen consumption in Weddell seals during aerobic dives
    R W Davis
    Department of Marine Biology, Texas A and M University at Galveston, Galveston, TX 77553, USA
    J Exp Biol 202:1091-113. 1999
    ....
  25. pmc Neuroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat Spalax
    Aaron Avivi
    Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
    Proc Natl Acad Sci U S A 107:21570-5. 2010
    ..A parallel regulatory response was found for myoglobin (Mb) in Spalax and rat muscle, suggesting similar functions of Mb and Ngb...
  26. ncbi Development of protein delivery microsphere system by a novel S/O/O/W multi-emulsion
    Weien Yuan
    Shanghai Jiaotong University, School of Pharmacy, 800 Dongchuan Road, Shanghai 200240, People s Republic of China
    Eur J Pharm Sci 36:212-8. 2009
    ..The dextran glassy particles can stabilize proteins in the PLGA matrix, which is the major advantage of this novel protein sustained-release system over preparation for the PLGA microspheres using W/O/W double-emulsion method...
  27. ncbi Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle
    Paul Robach
    Département médical, Ecole Nationale de Ski et d Alpinisme, Chamonix, France
    Blood 109:4724-31. 2007
    ..for oxygen transport because it is incorporated in the heme of the oxygen-binding proteins hemoglobin and myoglobin. An interaction between iron homeostasis and oxygen regulation is further suggested during hypoxia, in which ..
  28. pmc Staphylococcus aureus IsdB is a hemoglobin receptor required for heme iron utilization
    Victor J Torres
    Department of Microbiology and Immunology, Vanderbilt University Medical School, 21st Avenue South, Medical Center North, Room A 5211, Nashville, TN 37232, USA
    J Bacteriol 188:8421-9. 2006
    ..Here we report that S. aureus can utilize the host hemoproteins hemoglobin and myoglobin, but not hemopexin, as iron sources for bacterial growth...
  29. ncbi Serum myoglobin and renal morbidity and mortality following thoracic and thoraco-abdominal aortic repair: does rhabdomyolysis play a role?
    C C Miller
    Department of Cardiothoracic and Vascular Surgery, University of Texas Medical School at Houston, Memorial Hermann Heart and Vascular Institute, Houston, TX 77030, USA
    Eur J Vasc Endovasc Surg 37:388-94. 2009
    ..We studied postoperative myoglobinaemia and renal dysfunction following aortic surgery...
  30. ncbi Myoglobin plasma level related to muscle mass and fiber composition: a clinical marker of muscle wasting?
    Marc Andre Weber
    Department E010 Radiology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany
    J Mol Med (Berl) 85:887-96. 2007
    ..The present study evaluated the potential of myoglobin (MG) plasma levels to indicate wasting of large locomotor muscles and, moreover, to reflect the loss of MG-rich ..
  31. ncbi Prognostic value, kinetics and effect of CVVHDF on serum of the myoglobin and creatine kinase in critically ill patients with rhabdomyolysis
    T S Mikkelsen
    Department of Intensive Care, Aarhus University Hospital, Aarhus, Denmark
    Acta Anaesthesiol Scand 49:859-64. 2005
    I) To investigate the kinetics of the myoglobin and creatine kinase (CK) in rhabdomyolysis. Especially to describe those patients in whom an isolated increase in the myoglobin or the CK occurred at a later stage...
  32. pmc Molecular dynamics simulation of sperm whale myoglobin: effects of mutations and trapped CO on the structure and dynamics of cavities
    Cecilia Bossa
    Dipartimento di Chimica, University of Rome La Sapienza, Rome, Italy
    Biophys J 89:465-74. 2005
    ..of extended (80-ns) molecular dynamics simulations of wild-type and YQR triple mutant of sperm whale deoxy myoglobin in water are reported and compared with the results of the simulation of the intermediate(s) obtained by ..
  33. ncbi Myoglobin: an essential hemoprotein in striated muscle
    George A Ordway
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Exp Biol 207:3441-6. 2004
    b>Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O2 by its heme residue, a porphyrin ring:iron ion complex...
  34. ncbi Ligand pathways in myoglobin: a review of Trp cavity mutations
    J S Olson
    Department of Biochemistry and Cell Biology, Rice University, Texas 77005 1892, USA
    IUBMB Life 59:552-62. 2007
    The pathways for ligand entry and exit in myoglobin have now been well established by a wide variety of experimental results, including pico- to nano- to microsecond transient absorbance measurements and time-resolved X-ray ..
  35. ncbi Systematic relationships and biogeography of the tracheophone suboscines (Aves: Passeriformes)
    Martin Irestedt
    Department of Vertebrate Zoology and Molecular Systematics Laboratory, Swedish Museum of Natural History, P O Box 50007, SE 104 05 Stockholm, Sweden
    Mol Phylogenet Evol 23:499-512. 2002
    ..Both mitochondrial (cytochrome b) and nuclear genes (c-myc, RAG-1, and myoglobin) have been sequenced, and more than 3000 bp were subjected to parsimony and maximum-likelihood analyses...
  36. ncbi The escape process of carbon monoxide from myoglobin to solution at physiological temperature
    Yasutaka Nishihara
    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606 8502, Japan
    J Am Chem Soc 126:11877-88. 2004
    The carbon monoxide (CO) docking sites involved in the ligand escape process from the iron atom in hem of myoglobin (Mb) to solution at physiological temperature were studied on the basis of the effect of xenon (Xe) on the ligand escape ..
  37. ncbi Reduced albumin-cobalt binding with transient myocardial ischemia after elective percutaneous transluminal coronary angioplasty: a preliminary comparison to creatine kinase-MB, myoglobin, and troponin I
    D Bar-Or
    Swedish Medical Center
    Am Heart J 141:985-91. 2001
    ..We then compared ACB assay results with creatine kinase isoenzyme (CK-MB), myoglobin, and cardiac troponin I (cTn-I) values after PTCA.
  38. ncbi Redox reactions of hemoglobin and myoglobin: biological and toxicological implications
    A I Alayash
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Antioxid Redox Signal 3:313-27. 2001
    Direct cytotoxic effects associated with hemoglobin (Hb) or myoglobin (Mb) have been ascribed to redox reactions (involving either one- or two-electron steps) between the heme group and peroxides...
  39. ncbi Thyroid hormone stimulates myoglobin expression in soleus and extensorum digitalis longus muscles of rats: concomitant alterations in the activities of Krebs cycle oxidative enzymes
    R A dos Santos
    Department of Physiology and Biophysics, Institute of Biomedical Sciences, , Brazil
    Thyroid 11:545-50. 2001
    b>Myoglobin (Mb) gene expression, Citrate Synthase (CS) and Succinate Dehydrogenase (SDH) activities of Soleus (S) and Extensorum Digitalis Longus (EDL) muscles were studied in intact, thyroidectomized and T3-treated (25 microg/100g, BW, ..
  40. pmc Protein structural dynamics in solution unveiled via 100-ps time-resolved x-ray scattering
    Hyun Sun Cho
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    Proc Natl Acad Sci U S A 107:7281-6. 2010
    ..To demonstrate its capabilities, we have tracked structural changes in myoglobin as it undergoes a photolysis-induced transition from its carbon monoxy form (MbCO) to its deoxy form (Mb)...
  41. ncbi Use of a whole blood rapid panel test for heart-type fatty acid-binding protein in patients with acute chest pain: comparison with rapid troponin T and myoglobin tests
    Yoshihiko Seino
    First Department of Medicine and Coronary Care Unit, Nippon Medical School, Tokyo, Japan
    Am J Med 115:185-90. 2003
    ..We sought to determine the clinical utility of a newly developed qualitative test to measure heart-type fatty acid-binding protein levels in blood for the early identification of myocardial infarction...
  42. ncbi Roles of nitric oxide, nitrite and myoglobin on myocardial efficiency in trout (Oncorhynchus mykiss) and goldfish (Carassius auratus): implications for hypoxia tolerance
    Claus Lunde Pedersen
    Department of Biological Sciences, Universitetsparken, Aarhus University, DK 8000, Aarhus C, Denmark
    J Exp Biol 213:2755-62. 2010
    The roles of nitric oxide synthase activity (NOS), nitrite and myoglobin (Mb) in the regulation of myocardial function during hypoxia were examined in trout and goldfish, a hypoxia-intolerant and hypoxia-tolerant species, respectively...
  43. pmc The role of cavities in protein dynamics: crystal structure of a photolytic intermediate of a mutant myoglobin
    M Brunori
    Department of Biochemical Sciences, Consiglio Nazionale delle Ricerche Centre of Molecular Biology, University La Sapienza, 00185 Rome, Italy
    Proc Natl Acad Sci U S A 97:2058-63. 2000
    We determined the structure of the photolytic intermediate of a sperm whale myoglobin (Mb) mutant called Mb-YQR [Leu-(B10)-->Tyr; His(E7)-->Gln; Thr(E10)-->Arg] to 1...
  44. pmc Atomic level computational identification of ligand migration pathways between solvent and binding site in myoglobin
    Jory Z Ruscio
    Genetics, Bioinformatics, and Computational Biology Program, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Proc Natl Acad Sci U S A 105:9204-9. 2008
    b>Myoglobin is a globular protein involved in oxygen storage and transport...
  45. ncbi Osteocalcin and myoglobin removal in on-line hemodiafiltration versus low- and high-flux hemodialysis
    Francisco Maduell
    Department of Nephrology, Hospital General de Castellon, Spain
    Am J Kidney Dis 40:582-9. 2002
    ..Osteocalcin is a protein with a molecular mass of 5,800 daltons, and myoglobin is a large molecule with a molecular mass of 17,200 daltons...
  46. pmc Competition with xenon elicits ligand migration and escape pathways in myoglobin
    Catherine Tetreau
    Institut Curie Recherche INSERM U350, Centre Universitaire, 91405 Orsay, France
    Biophys J 86:435-47. 2004
    Evidence for ligand migration toward the xenon-binding cavities in myoglobin comes from a number of laser photolysis studies of MbO2 including mutants and from cryo- and time-resolved crystallography of MbCO...
  47. ncbi Myoglobin in pelagic small cetaceans
    M L Dolar
    Scripps Institution of Oceanography, La Jolla, CA 92093 0204, USA
    J Exp Biol 202:227-36. 1999
    Although myoglobin (Mb) is considered to contribute significantly to the oxygen and diving capacity of marine mammals, few data are available for cetaceans...
  48. ncbi Ligand migration in myoglobin: a combined study of computer simulation and x-ray crystallography
    Takayuki Tsuduki
    Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    J Chem Phys 136:165101. 2012
    A ligand-migration mechanism of myoglobin was studied by a multidisciplinary approach that used x-ray crystallography and molecular dynamics simulation...
  49. ncbi Variation in temperature tolerance among families of Atlantic salmon (Salmo salar) is associated with hypoxia tolerance, ventricle size and myoglobin level
    Katja Anttila
    Department of Zoology, 6270 University Boulevard, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4
    J Exp Biol 216:1183-90. 2013
    ..cellular levels, we also discovered support for the OCLTT concept as relative ventricle mass (RVM) and cardiac myoglobin (Mb) levels both correlated positively with CTmax (R(2)=0.21, P<0.001 and R(2)=0.17, P=0.003, respectively)...
  50. ncbi 'It's hollow': the function of pores within myoglobin
    Ayana Tomita
    Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro ku, Tokyo, 152 8551, Japan
    J Exp Biol 213:2748-54. 2010
    Despite a century of research, the cellular function of myoglobin (Mb), the mechanism regulating oxygen (O(2)) transport in the cell and the structure-function relationship of Mb remain incompletely understood...
  51. ncbi Evolution of mammalian diving capacity traced by myoglobin net surface charge
    Scott Mirceta
    Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK
    Science 340:1234192. 2013
    ..However, little is known regarding the molecular and evolutionary underpinnings of the high muscle myoglobin concentration phenotype of divers...
  52. ncbi Toxicity of myoglobin and haemoglobin: oxidative stress in patients with rhabdomyolysis and subarachnoid haemorrhage
    B J Reeder
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
    Biochem Soc Trans 30:745-8. 2002
    ..Haem-protein cross-linked myoglobin and haemoglobin are generated by ferric-ferryl redox cycling, and thus can be used as markers of oxidative ..
  53. ncbi Concentrations of myoglobin and myoglobin mRNA in heart ventricles from Antarctic fishes
    T J Moylan
    School of Marine Sciences, University of Maine, Orono, ME 04469 5741, USA
    J Exp Biol 203:1277-86. 2000
    ..approach to ascertain the presence and concentrations of both the intracellular oxygen-binding hemoprotein myoglobin (Mb) and its messenger RNA (mRNA) in 13 of 15 known species of Antarctic channichthyid icefishes...
  54. ncbi A longitudinal and cross-sectional analysis of total body oxygen store development in nursing harbor seals (Phoca vitulina)
    Cheryl A Clark
    Department of Biological Sciences, University of Alaska Anchorage, 3211 Providence Drive, Anchorage, AK 99508, USA
    J Comp Physiol B 177:217-27. 2007
    ..At each handling, hematocrit, hemoglobin, red blood cell count, total plasma volume, blood volume, muscle myoglobin concentration, and blood and muscle oxygen stores were determined...
  55. ncbi Determination of microscopic rate constants for CO binding and migration in myoglobin encapsulated in silica gels
    Silvia Sottini
    Dipartimento di Fisica, Universita degli Studi di Parma, Parco Area delle Scienze 7 A, Universita degli Studi di Parma, Parco Area delle Scienze 23 A, 43100 Parma, Italy
    J Phys Chem B 109:19523-8. 2005
    CO rebinding kinetics after nanosecond photolysis of myoglobin encapsulated in wet silica gels exhibits an enhanced geminate phase that allows the determination of the microscopic rate constants and the activation barriers for distinct ..
  56. pmc Cavities and packing defects in the structural dynamics of myoglobin
    M Brunori
    Istituto Pasteur Fondazione Cenci Bolognetti and Department of Biochemical Sciences A Rossi Fanelli, University of Rome La Sapienza, P le Aldo Moro 5, 00185 Rome, Italy
    EMBO Rep 2:674-9. 2001
    ..In the case of myoglobin (Mb), a prototype for structure-function relationship studies, the photosensitivity of the adduct of the reduced ..
  57. ncbi Preparing polymer-based sustained-release systems without exposing proteins to water-oil or water-air interfaces and cross-linking reagents
    Tuo Jin
    Shanghai JiaoTong University School of Pharmacy, China
    J Control Release 128:50-9. 2008
    ....
  58. ncbi Short-term immobilization after eccentric exercise. Part II: creatine kinase and myoglobin
    Stephen P Sayers
    Human Physiology Laboratory, Department of Health Sciences, Boston University, Sargent College of Health and Rehabilitation Sciences, 635 Commonwealth Avenue, Boston, MA 02215, USA
    Med Sci Sports Exerc 35:762-8. 2003
    ..In this study, we compared changes in plasma CK and myoglobin (Mb), as Mb is released from damaged muscle directly into the bloodstream whereas CK is released first into the ..
  59. pmc Folding intermediate and folding nucleus for I-->N and U-->I-->N transitions in apomyoglobin: contributions by conserved and nonconserved residues
    Ekaterina N Samatova
    Institute of Protein Research, Russian Academy of Sciences, Moscow, Russian Federation
    Biophys J 98:1694-702. 2010
    ....
  60. ncbi Heterogeneity of myoglobin distribution in the locomotory muscles of five cetacean species
    L K Polasek
    Department of Marine Biology, Texas A and M University at Galveston, Galveston, TX 77551, USA
    J Exp Biol 204:209-15. 2001
    b>Myoglobin is an important storage site for oxygen in the swimming muscles of diving marine mammals. However, little is known about its distribution within muscles since previous studies have relied on single samples...
  61. pmc Myoglobin's old and new clothes: from molecular structure to function in living cells
    Gerolf Gros
    Zentrum Physiologie, Medizinische Hochschule Hannover, 30625 Hannover, Germany
    J Exp Biol 213:2713-25. 2010
    b>Myoglobin, a mobile carrier of oxygen, is without a doubt an important player central to the physiological function of heart and skeletal muscle...
  62. ncbi Contribution of genetic influences to animal-to-animal variation in myoglobin content and beef lean color stability
    D A King
    Roman L Hruska US Meat Animal Research Center, USDA ARS, Clay Center, NE 68933 0166, USA
    J Anim Sci 88:1160-7. 2010
    ..b>Myoglobin concentration was highly heritable (0.85), but ultimate pH was weakly heritable (0.06)...
  63. ncbi Preparation of polysaccharide glassy microparticles with stabilization of proteins
    Weien Yuan
    Shanghai Jiaotong University, School of Pharmacy, Shanghai, 200240, China
    Int J Pharm 366:154-9. 2009
    ..Model protein (such as bovine serum albumin, myoglobin and beta-galactosidase (beta-Gal)) was dissolved in water together with dextran and polyethylene glycol (PEG), ..
  64. ncbi Preparation of protein-loaded sustained-release microspheres via 'solid-in-oil-in-hydrophilic oil-in-ethanol (S/O/hO/E)' emulsification
    Weien Yuan
    School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
    Colloids Surf B Biointerfaces 79:326-33. 2010
    ..The assay of enzymatic activity of beta-galactosidase in hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) indicated that over 90% of the protein recovered from the microspheres was active...
  65. ncbi Diagnostic value of serial measurement of cardiac markers in patients with chest pain: limited value of adding myoglobin to troponin I for exclusion of myocardial infarction
    Kai Marten Eggers
    Department of Cardiology, Cardiothoracic Center, University Hospital, Uppsala, Sweden
    Am Heart J 148:574-81. 2004
    ..Despite improved laboratory assays for cardiac markers and a revised standard for definition of myocardial infarction (AMI), early detection of coronary ischemia in unselected patients with chest pain remains a difficult challenge...
  66. ncbi The expression of myoglobin and ROR2 protein in Dupuytren's disease
    Minna Forsman
    Department of Surgery, Oulu University, Oulu, Finland
    J Surg Res 146:271-5. 2008
    ..The purpose of this study was to examine the gene expression profiles of palmar fascia of DD and healthy patients using microarray analysis to highlight the genes that might contribute to the pathogenesis of DD...
  67. ncbi Serum ratio of heart-type fatty acid-binding protein to myoglobin. A novel marker of cardiac damage and volume overload in hemodialysis patients
    Masato Furuhashi
    Second Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
    Nephron Clin Pract 93:C69-74. 2003
    ..We evaluated whether the serum ratio of hFABP to myoglobin (F/M) might be a useful marker for assessing cardiac damage in hemodialysis patients.
  68. ncbi Acute inhibition of myoglobin impairs contractility and energy state of iNOS-overexpressing hearts
    Carsten Wunderlich
    Institute for Cardiovascular Physiology, Heinrich Heine University, Dusseldorf, Germany
    Circ Res 92:1352-8. 2003
    ..1H nuclear magnetic resonance (NMR) spectroscopy was used to monitor changes of cardiac myoglobin (Mb) metabolism in isolated hearts of wild-type (WT) and TGiNOS mice...
  69. ncbi Enhanced sampling by multiple molecular dynamics trajectories: carbonmonoxy myoglobin 10 micros A0-->A(1-3) transition from ten 400 picosecond simulations
    Anne E Loccisano
    The National Energy and Technology Laboratory, Pittsburgh, PA 15236 0940, USA
    J Mol Graph Model 22:369-76. 2004
    ..the time scale of molecular dynamics simulations is reported for the spectroscopic A-states of carbonmonoxy myoglobin (MbCO)...
  70. ncbi Comparison of the new high sensitive cardiac troponin T with myoglobin, h-FABP and cTnT for early identification of myocardial necrosis in the acute coronary syndrome
    Kerstin Kurz
    Medizinische Klinik, Abteilung für Innere Medizin III, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 410, Heidelberg, Germany
    Clin Res Cardiol 100:209-15. 2011
    ..T assay (TnThs) for early diagnosis of myocardial infarction in patients with suspected acute coronary syndrome (ACS) and compare it with the fourth generation cTnT assay, myoglobin and heart-type fatty acid binding protein (h-FABP).
  71. ncbi Free-energy landscape, principal component analysis, and structural clustering to identify representative conformations from molecular dynamics simulations: the myoglobin case
    Elena Papaleo
    Department of Biotechnology and Bioscience, University of Milano Bicocca, 20126 Milan, Italy
    J Mol Graph Model 27:889-99. 2009
    ..dynamics (MD) simulations were used to sample conformations in the neighborhood of the native structure of holo-myoglobin (holo-Mb), collecting trajectories spanning 0.22 micros at 300 K...
  72. ncbi Spin-forbidden CO ligand recombination in myoglobin
    Jeremy N Harvey
    School of Chemistry, University of Bristol, Cantock s Close, Bristol, UK BS8 1TS
    Faraday Discuss 127:165-77. 2004
    The reaction of small ligands within the distal pocket of haem proteins such as myoglobin, to form ligated, low-spin iron complexes is an archetypal spin-forbidden process in bioinorganic chemistry, because the initial, "deoxy" iron ..
  73. pmc Theoretical models of microvascular oxygen transport to tissue
    Daniel Goldman
    Department of Medical Biophysics, The University of Western Ontario, London, Ontario, Canada
    Microcirculation 15:795-811. 2008
    ..Finally, the issue of experimental validation of microvascular O(2) delivery models is discussed, and new directions in O(2) transport modeling are outlined...
  74. ncbi Mapping the pathways for O2 entry into and exit from myoglobin
    E E Scott
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA
    J Biol Chem 276:5177-88. 2001
    ..90 mutants at 27 different positions were used to map pathways for ligand movement into and out of sperm whale myoglobin. By analogy to a baseball glove, the protein "catches" and then "holds" incoming ligand ..
  75. ncbi Nitrite reductase function of deoxymyoglobin: oxygen sensor and regulator of cardiac energetics and function
    Tienush Rassaf
    University Hospital Aachen, Department of Medicine, Division of Cardiology, Pulmonary and Vascular Diseases, Aachen, Germany
    Circ Res 100:1749-54. 2007
    Although the primary function of myoglobin (Mb) has been considered to be cellular oxygen storage and supply, recent studies have suggested to classify Mb as a multifunctional allosteric enzyme...
  76. ncbi The ontogeny of aerobic and diving capacity in the skeletal muscles of Weddell seals
    S B Kanatous
    Department of Biology, Colorado State University, Fort Collins, CO 80523 1878, USA
    J Exp Biol 211:2559-65. 2008
    ..were collected from pups, juveniles and adults and analyzed for changes in fiber type, mitochondrial density, myoglobin concentrations and aerobic, lipolytic and anaerobic enzyme activities...
  77. pmc Modeling of oxygen transport and cellular energetics explains observations on in vivo cardiac energy metabolism
    Daniel A Beard
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS Comput Biol 2:e107. 2006
    ..In addition, the validated model provides quantitative predictions of changes in phosphate metabolites during cardiac ischemia...
  78. pmc Myoglobin: A scavenger of bioactive NO
    U Flogel
    Institute for Cardiovascular Physiology, Heinrich Heine University, 40225 Dusseldorf, Germany
    Proc Natl Acad Sci U S A 98:735-40. 2001
    The present study explored the role of myoglobin (Mb) in cardiac NO homeostasis and its functional relevance by employing isolated hearts of wild-type (WT) and myoglobin knockout mice...
  79. ncbi Peak value of blood myoglobin predicts acute renal failure induced by rhabdomyolysis
    Shunji Kasaoka
    Advanced Medical Emergency and Critical Care Center, Yamaguchi University Hospital, Ube, Yamaguchi 755 8505, Japan
    J Crit Care 25:601-4. 2010
    Acute renal failure (ARF) is the most important complication of rhabdomyolysis. Serial measurements of blood myoglobin might be useful for predicting rhabdomyolysis-induced ARF.
  80. ncbi His64(E7)-->Tyr apomyoglobin as a reagent for measuring rates of hemin dissociation
    M S Hargrove
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251 1892
    J Biol Chem 269:4207-14. 1994
    To develop an assay for hemin dissociation, His64(E7) was replaced by Tyr in sperm whale myoglobin producing a holoprotein with a distinct green color due to an intense absorption band at 600 nm...
  81. ncbi Tryptophanyl substitutions in apomyoglobin determine protein aggregation and amyloid-like fibril formation at physiological pH
    Ivana Sirangelo
    Dipartimento di Biochimica e Biofisica, Seconda Universita degli Studi di Napoli, Via L De Crecchio 7, 80138 Napoli, Italy
    J Biol Chem 277:45887-91. 2002
    b>Myoglobin is an alpha-helical globular protein that contains two highly conserved tryptophan residues located at positions 7 and 14 in the N-terminal region of the protein...
  82. ncbi Fibrillogenesis and cytotoxic activity of the amyloid-forming apomyoglobin mutant W7FW14F
    Ivana Sirangelo
    Dipartimento di Biochimica e Biofisica, Seconda Universita degli Studi di Napoli, Via L De Crecchio 7, 80138 Naples, Italy
    J Biol Chem 279:13183-9. 2004
    ..Propidium iodide uptake showed that cell toxicity is the result of altered membrane permeability. Phalloidin staining showed that membrane damage is not associated to an altered cell shape caused by changes in the cytoskeleton...
  83. ncbi Myoglobin facilitates oxygen diffusion
    M W Merx
    Institut für Herz und Kreislaufphysiologie, Heinrich Heine Universitat Dusseldorf, Germany
    FASEB J 15:1077-9. 2001
  84. ncbi Three-dimensional Fourier synthesis of horse oxyhaemoglobin at 2.8 A resolution: the atomic model
    M F Perutz
    Nature 219:131-9. 1968
  85. ncbi A causative role for redox cycling of myoglobin and its inhibition by alkalinization in the pathogenesis and treatment of rhabdomyolysis-induced renal failure
    K P Moore
    Joint Department of Medicine, Royal Free and University College Medical School, London NW3 2QG, United Kingdom
    J Biol Chem 273:31731-7. 1998
    Muscle injury (rhabdomyolysis) and subsequent deposition of myoglobin in the kidney causes renal vasoconstriction and renal failure...
  86. ncbi Tetracycline inhibits W7FW14F apomyoglobin fibril extension and keeps the amyloid protein in a pre-fibrillar, highly cytotoxic state
    Clorinda Malmo
    Dipartimento di Biochimica e Biofisica, Seconda Universita di Napoli, Naples, Italy
    FASEB J 20:346-7. 2006
    ..Thus, this inhibition keeps the W7FW14F mutant in a prefibrillar, highly cytotoxic state. In this respect, a careful usage of tetracycline as fibril inhibitor is indicated...
  87. ncbi Heme proteins: the role of solvent in the dynamics of gates and portals
    Mariano Andrea Scorciapino
    Department of Chemical Sciences, University of Cagliari, Italy
    J Am Chem Soc 132:5156-63. 2010
    ..of solvation on proteins dynamics have been investigated by employing molecular dynamics simulations and using myoglobin as a model system...
  88. ncbi Ninety-minute exclusion of acute myocardial infarction by use of quantitative point-of-care testing of myoglobin and troponin I
    J McCord
    Henry Ford Hospital Center, 2799 W Grand Blvd, Detroit, MI 48202, USA
    Circulation 104:1483-8. 2001
    ..The present study evaluated whether a multimarker strategy that used point-of-care measurement of myoglobin, creatine kinase (CK)-MB, and troponin I could exclude acute myocardial infarction in </=3 hours.
  89. ncbi CO escape from myoglobin with metadynamics simulations
    Matteo Ceccarelli
    CNR INFM SLACS and Department of Physics, University of Cagliari, SP Monserrato Sestu Km 0 700, I 09042 Monserrato, Italy
    Proteins 71:1231-6. 2008
    The relatively small size of myoglobin makes it suitable for the investigation of the ligand escape process in respiratory proteins and, in general, an ideal model system for the study of the more general structure-function paradigm...
  90. ncbi Comparison between leg and arm eccentric exercises of the same relative intensity on indices of muscle damage
    Athanasios Z Jamurtas
    Department of Physical Education and Sport Science, University of Thessaly, Karies, Trikala, Greece
    Eur J Appl Physiol 95:179-85. 2005
    ..delayed onset muscle soreness (DOMS), serum creatine kinase (CK) and lactate dehydrogenase (LDH) activities, myoglobin (Mb) concentration, and muscle strength [EPT and isometric peak torque (IPT)] were assessed before and 24, 48, ..
  91. pmc Pathway for heme uptake from human methemoglobin by the iron-regulated surface determinants system of Staphylococcus aureus
    Hui Zhu
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59718, USA
    J Biol Chem 283:18450-60. 2008
    ..Hemin is then relayed to IsdE, the lipoprotein component of the IsdDEF transporter...
  92. ncbi When bad things happen to good fish: the loss of hemoglobin and myoglobin expression in Antarctic icefishes
    Bruce D Sidell
    School of Marine Sciences, University of Maine, 5751 Murray Hall, Orono, ME 04469 5751, USA
    J Exp Biol 209:1791-802. 2006
    ..Their loss of hemoglobin (Hb) expression, and in some cases, loss of myoglobin (Mb) expression, has taught us much about the function of these proteins...
  93. ncbi The sensitivity of cardiac markers: an evidence-based approach
    M Panteghini
    1 Laboratorio Analisi Chimico cliniche, Spedali Civili, Brescia, Italy
    Clin Chem Lab Med 37:1097-106. 1999
    ..In particular, the diagnostic sensitivities of myoglobin, creatine kinase (CK)-MB isoenzyme, determined as mass concentration, CK-MB isoforms, and of the two cardiac ..
  94. pmc Hypoxia reprograms calcium signaling and regulates myoglobin expression
    Shane B Kanatous
    Department of Biology, Colorado State University, Fort Collins, CO, USA
    Am J Physiol Cell Physiol 296:C393-402. 2009
    b>Myoglobin is an oxygen storage molecule that is selectively expressed in cardiac and slow-twitch skeletal muscles that have a high oxygen demand...
  95. ncbi The synthetic polyphenol tert-butyl-bisphenol inhibits myoglobin-induced dysfunction in cultured kidney epithelial cells
    Anu Shanu
    Discipline of Pathology, Redox Biology Group, The University of Sydney, Sydney, NSW, 2006, Australia
    Free Radic Res 44:843-53. 2010
    Abstract Rhabdomyolysis caused by severe burn releases extracellular myoglobin (Mb) that accumulates in the kidney and urine (maximum [Mb] approximately 50 microM) (termed myoglobinuria). Extracellular Mb can be a pro-oxidant...
  96. ncbi Metabolic and vascular support for the role of myoglobin in humans: a multiparametric NMR study
    S Duteil
    NMR Laboratory AFM CEA, IFR 14, Institute of Myology, Pitie Salpetriere University Hospital, Paris Cedex, France
    Am J Physiol Regul Integr Comp Physiol 287:R1441-9. 2004
    In human muscle the role of myoglobin (Mb) and its relationship to factors such as muscle perfusion and metabolic capacity are not well understood...
  97. ncbi Myoglobin protects the heart from inducible nitric-oxide synthase (iNOS)-mediated nitrosative stress
    Axel Gödecke
    Institut für Herz und Kreislaufphysiologie, Heinrich Heine Universitat, Postfach 101007, 40001 Dusseldorf, Germany
    J Biol Chem 278:21761-6. 2003
    ..Here we report that transgenic mice with cardiac specific iNOS-overexpression and concomitant myoglobin-deficiency (tg-iNOS+/myo-/-) develop signs of heart failure with cardiac hypertrophy, ventricular dilatation, ..
  98. ncbi Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens
    Seobo Sim
    Department of Parasitology and Institute of Tropical Medicine, BK21 Project for Medical Science, Yonsei University College of Medicine, 120 752, Seoul, Korea
    Parasitol Res 91:338-43. 2003
    ..The selected genes, which were named C. sinensis myoglobin, contained a single open reading frame of 450 base pairs encoding 150 amino acids. A single hybridized band of 0...
  99. pmc Extended molecular dynamics simulation of the carbon monoxide migration in sperm whale myoglobin
    Cecilia Bossa
    Department of Chemistry, University of Rome La Sapienza, Rome, Italy
    Biophys J 86:3855-62. 2004
    ..molecular dynamics simulation on the migration of photodissociated carbon monoxide in wild-type sperm whale myoglobin. Our results allow following one possible ligand migration dynamics from the distal pocket to the Xe1 cavity via ..
  100. pmc X-ray structure analysis of a metalloprotein with enhanced active-site resolution using in situ x-ray absorption near edge structure spectroscopy
    Alessandro Arcovito
    Department of Biochemical Sciences and Consiglio Nazionale delle Ricerche, Institute of Molecular Biology and Pathology, University of Rome La Sapienza, P le A Moro 5, 00185 Rome, Italy
    Proc Natl Acad Sci U S A 104:6211-6. 2007
    ..and Fe K-edge polarized x-ray absorption near edge structure (XANES) spectra on the same cyanomet sperm whale myoglobin crystal...
  101. ncbi Myoglobin, desmin and vimentin in ultrastructurally proven rhabdomyomas and rhabdomyosarcomas. An immunohistochemical study utilizing a series of monoclonal and polyclonal antibodies
    T Seidal
    Department of Pathology, Sahlgren Hospital, Goteborg, Sweden
    Appl Pathol 5:201-19. 1987
    The present study is an immunohistochemical analysis utilizing a series of mono- and polyclonal antibodies to myoglobin, desmin and vimentin in smooth and striated control muscle tissues, 7 alveolar and 7 embryonal rhabdomyosarcomas and ..

Research Grants62

  1. Biosynthetic Protein Models of Heme-copper Oxidases and Nitric Oxide Reductases
    Yi Lu; Fiscal Year: 2013
    ..a novel biosynthetic approach that utilizes a stable, easy-to-produce, and well-characterized heme protein (myoglobin) as a scaffold for making structural and functional models of HCO and NOR...
  2. Research Center for Pharmacology &Drug Toxicology
    L Jackson Roberts; Fiscal Year: 2013
    ..hemorrhage and the second project will evaluate the ability of acetaminophen to inhibit hemoglobin and myoglobin induced oxidative stress in patients undergoing cardiopulmonary bypass surgery...
  3. Novel endonuclease-targeted approaches to nephroprotection
    Alexei G Basnakian; Fiscal Year: 2013
    ..We plan to use complimentary in vivo (glycerol-induced rhabdomyolysis) and in vitro (hemin or myoglobin) approaches to induce tubular epithelial cells injury...
  4. Nitrite Mediated Cardioprotection
    DAVID JOSEPH LEFER; Fiscal Year: 2012
    ..our mechanistic studies of nitrite supplementation therapy by investigating the reduction of nitrite to NO by myoglobin. Specific Aim 3 will investigate some of the molecular mechanisms of nitrite supplementation therapy, including ..
  5. Myoglobin as a Nitrite Reductase that Regulates Hypoxic Cardiac NO Signaling
    Sruti Shiva; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): There is an emerging appreciation that heme proteins such as myoglobin and hemoglobin catalyze the metabolism of nitric oxide (NO) and nitrite, and thereby modulate cell and tissue responses to ..
  6. Development of an instrument to track oxygen from blood to mitochondria in vivo
    Kenneth A Schenkman; Fiscal Year: 2013
    ..provide snapshots of tissue oxygen profiles (TOPs) in muscle by simultaneously measuring hemoglobin saturation, myoglobin saturation, and cytochrome aa3 oxidation from optical reflectance spectra...
  7. Molecular Probes of the Mechanisms of Cytocrhome P450
    JOHN TAYLOR GROVES; Fiscal Year: 2013
    ..of the mechanisms of action of the drug-metabolizing enzymes cytochrome P450 and other heme proteins such as myoglobin and cytochrome c...
  8. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph R Lakowicz; Fiscal Year: 2013
    ..Using these structures we have reached the needed clinical sensitivity goals for myoglobin, and are within a factor of 10 of the sensitivity needed for B-type natriuretic peptide (BNP) and cardiac ..
  9. Femtosecond Coherence Spectroscopy &Ultrafast Kinetic Study of Heme Proteins
    Paul M Champion; Fiscal Year: 2012
    ..For example, the two geminate phases for oxygen rebinding to the heme in myoglobin exhibit very different Arrhenius prefactors, suggesting that the entropic barrier for recombination is time ..
  10. Biomarkers of sick sinus syndrome
    Liang Tang; Fiscal Year: 2013
    ..An elevated cTnI concentration was reported to be associated with SSS. A combination of cTnI and myoglobin, one of the earliest biomarkers released into blood circulation, can achieve diagnostic sensitivity of 97% with ..
  11. Structural Contexts and Functional Consequences of Protein S-nitrosylation
    Eric R Schreiter; Fiscal Year: 2010
    ..We have previously demonstrated using X-ray crystallography that S- nitrosylation of myoglobin induces a conformational change in that protein...
  12. Microfluidics for High-Throughput HDX-MS
    Julian P Whitelegge; Fiscal Year: 2013
    ..Our joint team has demonstrated automated multi-step HDX experiments with two model proteins, myoglobin (Mb) and bacteriorhodopsin (bR, an integral membrane protein) using the first-generation DMDG chips coupled with ..
  13. Iron homeostasis in mammalian muscle
    Nancy Catherine Andrews; Fiscal Year: 2013
    ..We focused on skeletal and cardiac muscle because both tissues are highly metabolic and require iron for myoglobin production, mitochondrial biogenesis and mitochondrial function...
  14. Point-of-care system based on single polymer nanowires for real-time, AMI diagnos
    Minhee Yun; Fiscal Year: 2010
    ..Utilizing the novel concepts, a model sensing system is proposed to develop four represented cardiac markers (myoglobin, B-type natriuretic peptide, cardiac troponin I, and creatin kinase-MB)...
  15. Mechanisms of photobiomodulation in myocardial reperfusion injury
    Martin Bienengraeber; Fiscal Year: 2013
    ..Three Specific Aims will be used to identify the potential contributions of NO containing myoglobin, hemoglobin and/or cytochrome c oxidase to the cardioprotective effect of NIR in the non-diabetic and diabetic ..
  16. Modulation of In Vivo Tumor Oxygenation via Polymersome-encapsulated Myoglobin
    PAIMAN PETER GHOROGHCHIAN; Fiscal Year: 2011
    ..Significant additional advantages include tremendous cost savings to the health care system, in reduced operating and therapeutic costs, as well as local job creation and economic stimulus. ..
  17. Electrostatically triggered hydrophobic self-assembly of protein hydrogels
    Kevin Baler; Fiscal Year: 2013
    ..This proposal will utilize this new understanding of induced protein self-assembly to develop a new albumin/myoglobin-based scaffold that can deliver oxygen on demand by neighboring cells...
  18. Rapid homogeneous antibody-based detection of proteins
    Tomasz Heyduk; Fiscal Year: 2010
    ..molecular pincer assays for six markers of cardiac disease (troponin I, B-type natriuretic peptide (BNP), myoglobin, creatine kinase (CK-MB), C-reactive protein (CRP), and D-dimer) for research market...
  19. Catalytic Properties of Cytochrome Oxidase
    Denis Rousseau; Fiscal Year: 2009
    ..In addition, the oxygen chemistry in a new class of model complexes, a mutant myoglobin with a binuclear center engineered in it to mimic the catalytic site of CcO, will be studied to elucidate the ..
  20. Neuroglobin in Neuronal Hypoxi and Cerebral Ischemia
    DAVID ALAN GREENBERG; Fiscal Year: 2013
    ..Neuroglobin (Ngb), a recently discovered monomeric globin that is distantly related to hemoglobin and myoglobin, is expressed predominantly in brain neurons, and appears to modulate hypoxic-ischemic brain injury...
  21. METABOLISM AND NUTRITION OF IRON AND OTHER TRACE METALS
    PAUL SALTMAN; Fiscal Year: 1993
    ..Heme proteins including hemoglobin, myoglobin and cytochrome beta5 reduce complexes of Fe(lll) and Cu(ll)...
  22. HNO Interactions with Metalloproteins: Structures and Mechanisms
    Yong Zhang; Fiscal Year: 2013
    ..Aim 1 is to determine the atomic level HNO binding modes in myoglobin (Mb). Heme proteins are frequently involved in HNO binding...
  23. ELECTRON TRANSFER WITHIN PROTEIN COMPLEXES
    Brian M Hoffman; Fiscal Year: 2013
    ..The three systems to be studied involve distinct interfaces: myoglobin (Mb), the vehicle for protein redesign efforts, binds to electron acceptor partner proteins primarily through ..
  24. Nitrogen Oxides and Heme
    GEORGE RICHTER ADDO; Fiscal Year: 2007
    ..Interestingly, myoglobin (Mb) and hemoglobin have recently been proposed to act as nitrite reductases under hypoxic conditions, ..
  25. Approaches to computing diffusion rates in proteins from transition path theory
    Cameron F Abrams; Fiscal Year: 2013
    ..We consider three major penetrant/protein systems: (i) CO/myoglobin;(ii) O2/monomeric sarcosine oxidase (MSOX);and (iii) H2O/aquaporin-1...
  26. Coboglobins-Cobalt-Substituted Hemoglobins and Myoglobin
    Brian M Hoffman; Fiscal Year: 2013
    ..abstract_text> ..
  27. PORPHYRINS AND QUINONES AS ENZYME COFACTORS
    THOMAS LOEHR; Fiscal Year: 1999
    DESCRIPTION: In humans and animals, the heme released from hemoglobin, myoglobin, and cytochrome P-450 degradation is converted to open-chain biliverdin by heme oxygenase (HO) catalysis...
  28. Multi-analyte Biosensor for Emerging Cardiac Markers
    CHARLES PENNINGTON; Fiscal Year: 2001
    ..for rapid quantification of conventional markers including, cardiac troponin I, total creatinase kinase MB, and myoglobin attempt to address this need, but only identify downstream effects of a plaque rupture event...
  29. Nitroxyl adducts as structural probes of oxygenase/substrate interactions
    Thomas Poulos; Fiscal Year: 2009
    ..HNO is isoelectronic to O2, and is shown to bind tightly and irreversibly to O2 carriers like myoglobin, hemoglobin and leghemoglobin...
  30. DETERMINANTS OF FUNCTIONAL DIVERSITY IN HEME ENZYMES
    DAVID GOODIN; Fiscal Year: 1993
    ..those of another by alteration of substrate specificities, or to those of another class of heme protein such as myoglobin by alteration of the protein environment of the heme active site...
  31. ELECTROSTATICS AND DYNAMICS IN MYOGLOBIN MUTANTS
    STEVEN BOXER; Fiscal Year: 2003
    ..b>Myoglobin (Mb) was chosen because it is an extremely well characterized, stable protein for which the genes and excellent ..
  32. NO DYNAMICS IN HEMEPROTEINS--TRANSIENT INFRARED STUDIES
    Marco Pereira; Fiscal Year: 1999
    ..In the Phe29 mutant myoglobin, the Phe29 residue acts as a very high barrier to ligand diffusion, separating the ligands into two groups: the ..
  33. A Model-Based Estimate of Carbon Monoxide Uptake by Heart Muscle During Exercise
    Eugene Bruce; Fiscal Year: 2007
    ..the common treatment strategy for toxic CO exposures, our model predicts a further rise in CO uptake by muscle myoglobin (Mb), even as COHb levels fall, suggesting an increased risk for hypoxic injury to the myocardium...
  34. INTERACTION OF PRESSURIZED GASES WITH PROTEINS
    Robert Tilton; Fiscal Year: 1990
    ..interactions of several inert gases (He, Ar, Xe, CF4, SF6, N2) with the oxygen binding and transport proteins myoglobin and hemoglobin...
  35. STRUCTURAL AND DYNAMIC STUDY OF MODEL HEME COMPLEXES
    GERD LA MAR; Fiscal Year: 2004
    ..approaches to, and application of, NMR spectroscopy of particularly paramagnetic derivatives of O2 binding myoglobin and hemoglobin will be explored and expanded to elucidate electronic and molecular structural control mechanisms ..
  36. Rexinoid-Regulated Muscle Gene Expression
    Peter Davies; Fiscal Year: 2004
    ..regulate the expression of genes associated with the "slow twitch" (Type I) myofiber phenotype (such as myoglobin and Troponin I slow) and the enzymes of oxidative fatty acid metabolism...
  37. STRUCTURAL DETERMINANTS OF LIGAND BINDING TO MYOGLOBIN
    George Phillips; Fiscal Year: 1999
    The general goals of this project are to establish solid structure- function relationships in ligand binding to myoglobin that include definitions of the roles of key amino acid residues, the roles of dynamics and conformational ..
  38. CARRIER-MEDIATED O2 TRANSPORT IN ISOLATED HEART CELLS
    Beatrice Wittenberg; Fiscal Year: 1991
    In intact heart, myoglobin inactivation reduces the maximal work output and oxygen consumption. In isolated heart cells, we show a 30% reduction in respiration and decreased ATP production when myoglobin is titrimetrically inactivated...
  39. PORPHYRIN INDUCED CA2+ RELEASE FROM SR
    JONATHAN ABRAMSON; Fiscal Year: 1990
    ..When coordinated to a central metal ion, porphyrins perform such functions as oxygen transport and storage (myoglobin and hemoglobin), electron and energy transfer (cytochromes and chlorophylls), and biocatalysis (coenzyme B12, ..
  40. GENETICS, STRUCTURE, AND EXPRESSION OF IA ANTIGENS
    Patricia Jones; Fiscal Year: 1993
    ..azobenzenearsonate-tyro-sine, or peptides from hen egg lysozyme, and I-E-restricted T cells specific for myoglobin. The latter will be used to explore the functional significance of the absence of polymorphism in the E alpha ..
  41. Myoglobin as an indicator of hpoxia tolerance
    STEPHEN KINSEY; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): This study will assess the utility of using changes in myoglobin (Mb) concentration in response to chronic hypoxia as a diagnostic tool for assessing the balance between oxygen supply ..
  42. ELECTROCHEMICAL BIOSENSOR OF CARDIAC MARKERS
    Kai Wang; Fiscal Year: 2000
    ..immunochromatographic electrochemical biosensor(IEB) is proposed for simultaneous quantitation of Troponin-I, Myoglobin and CK-MB, which have been reported to be important indexes in the diagnosis of myocardial infraction...
  43. MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTS
    GALEN PIEPER; Fiscal Year: 2004
    ..These proteins include myoglobin and aconitase...
  44. CONTROL OF 02 TRANSPORT IN HEART AND SKELETAL MUSCLES
    THOMAS GAYESKI; Fiscal Year: 1992
    ..It has already been used to map gradients in myoglobin (Mb) saturation in individual myocytes...
  45. MYOGLOBIN DETECTION AND OXIDATIVE METABOLISM
    Kamil Ugurbil; Fiscal Year: 2002
    ..proposal continues this strategy and is centered around the use of 1h NMR techniques to evaluate myocardial myoglobin deoxygenation in vivo for the first time, combined with 31 P NMR measurements of high energy phosphate compound ..
  46. DECONVOLUTION OF LIGAND BINDING TO HEMOPROTEINS
    Marco Lopez; Fiscal Year: 1992
    ..Recent picosecond and nanosecond kinetic studies on the geminate recombination of photodissociated CO to Myoglobin, and Myoglobin mutants have raised fundamental questions concerning the influence of different factors on the ..
  47. HEMOGLOBIN METABOLISM--CLINICAL AND BASIC APPROACH
    URSULA MULLER EBERHARD; Fiscal Year: 1980
    ..the interaction with the hepatocyte membrane and the antibody binding? What is the function of hemopexin in myoglobin metabolism? And how is the synthesis of this protein regulated? We plan to study hemopexin's function in heme ..
  48. FUNCTIONAL ANALOGUES OF HEMERYTHRIN
    Brice Bosnich; Fiscal Year: 2000
    ..Higher organisms use iron coordinated to a heme (hemoglobin, myoglobin) to absorb and transport molecular oxygen. Other organisms use proximal copper ions to serve the same function...
  49. THEORETICAL STUDIES OF LIGAND BINDING TO HEME PROTEINS
    David Case; Fiscal Year: 1990
    ..Such events are of physiological importance for the oxygen storage and transport proteins myoglobin and hemoglobin, and are models of the ways in which internal cavities in proteins accomodate themselves to "..
  50. EARLY EVENTS IN PROTEIN FOLDING
    Denis Rousseau; Fiscal Year: 2006
    ..technique, we have been able to observe the folding of a lipid binding protein, cytochrome c and sperm whale apo-myoglobin in the submillisecond time domain for the first time...
  51. Bacteria-Based MR Molecular Imaging of Tumor Oxygenation
    Qiuhong He; Fiscal Year: 2004
    ..b>Myoglobin will be genetically engineered into the Salmonella bacteria as an oxygen sensor...
  52. Regulation of Tissue Hemoglobins in the Heart
    PRADEEP MAMMEN; Fiscal Year: 2009
    b>Myoglobin is a monomeric, cytoplasmic hemoprotein expressed exclusively in cardiomyocytes and oxidative skeletal myofibers...
  53. Novel Cardioprotective Mechanisms Myoglobin null Mice
    Daniel Garry; Fiscal Year: 2005
    ..b>Myoglobin is a unique hemoprotein...
  54. Probing the Structure of Transient Unfolded States with NMR Relaxation Dispersion
    DERRICK WALTER MEINHOLD; Fiscal Year: 2010
    ..The basic goal of the proposed research is to map the native-state energy landscape of apo-myoglobin using NMR R2 dispersion techniques, with the applied goal of developing a new model system for understanding ..
  55. TERNARY COMPLEX THAT DRIVES T CELL ACTIVATION
    C Fathman; Fiscal Year: 1993
    ..analyzed using murine T cell clones specific for well defined epitopes on the model protein antigen sperm whale myoglobin (Spw Mb)...
  56. IMMUNOLOGICAL STUDIES OF MUSCLE AND MYOGLOBIN
    Lawrence Kagen; Fiscal Year: 1980
    The biochemistry and physiology of myoglobin are studied through the application of immunological techniques...
  57. MYOGLOBIN LIGATION AFTER RAPID ELECTRON TRANSFER
    D Rillema; Fiscal Year: 1990
    The interaction of myoglobin with ligands such as 02. or CO is of obvious biological significance. However, certain details of ligand binding/dissociation are not clearly understood...
  58. FIBEROPTIC REFLECTANCE/PHOSPHORESCENCE DECAY SPECTROMETE
    CLYDE BARLOW; Fiscal Year: 1992
    ..three major pools - as oxygen dissolved in aqueous media, as oxygen bound to hemoblobin and as oxygen bound to myoglobin. In this proposal, instrumentation will be developed to simultaneously obtain reflectance spectra and ..
  59. Multifunctional Nanoparticles for Intracellular Delivery
    Stefan Franzen; Fiscal Year: 2006
    ..5. Develop strategies for covalent attachment of proteins and oligonucleotides to nanoparticles. 6. Determine of the efficiency of regulated in vitro protein expression using a well-characterized model system. ..
  60. Cardiac Troponin Testing for Myocardial Infarction
    Fred Apple; Fiscal Year: 2001
    ..abstract_text> ..
  61. Mitochondrial Dysfunction in Aged Muscle
    David J Marcinek; Fiscal Year: 2010
    ..2) Our long-term goal is the development of non- invasive methods to diagnose mitochondrial dysfunction and follow the progress of interventions meant to reverse disability in the elderly. ..
  62. PEDIATRIC CARDIOMYOPATHY REGISTRY
    Steven Lipshultz; Fiscal Year: 2009
    ..These studies will dramatically increase our understanding of pediatric cardiomyopathy, suggest new areas of research and improved patient management. ..