helminth proteins

Summary

Summary: Proteins found in any species of helminth.

Top Publications

  1. ncbi Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    A Fire
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Nature 391:806-11. 1998
  2. ncbi Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nature 421:231-7. 2003
  3. pmc Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity
    James P Hewitson
    Centre for Immunity, Infection and Evolution, Institute of Immunology and Infection Research, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 167:1-11. 2009
  4. ncbi SMEDWI-2 is a PIWI-like protein that regulates planarian stem cells
    Peter W Reddien
    Howard Hughes Medical Institute, Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Science 310:1327-30. 2005
  5. pmc Beta-catenin defines head versus tail identity during planarian regeneration and homeostasis
    Kyle A Gurley
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, 401 MREB, 20N 1900E, Salt Lake City, UT 84132, USA
    Science 319:323-7. 2008
  6. pmc Evaluation of urine CCA assays for detection of Schistosoma mansoni infection in Western Kenya
    Hillary L Shane
    Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
    PLoS Negl Trop Dis 5:e951. 2011
  7. pmc The major component in schistosome eggs responsible for conditioning dendritic cells for Th2 polarization is a T2 ribonuclease (omega-1)
    Svenja Steinfelder
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 206:1681-90. 2009
  8. pmc Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses
    Bart Everts
    Department of Parasitology, Leiden University Medical Centre, Leiden 2333 ZA, The Netherlands
    J Exp Med 206:1673-80. 2009
  9. pmc A wound-induced Wnt expression program controls planarian regeneration polarity
    Christian P Petersen
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:17061-6. 2009
  10. pmc Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling
    Sasisekhar Bennuru
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Negl Trop Dis 3:e410. 2009

Research Grants

Detail Information

Publications254 found, 100 shown here

  1. ncbi Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    A Fire
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Nature 391:806-11. 1998
    ....
  2. ncbi Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nature 421:231-7. 2003
    ..Our resulting data set and reusable RNAi library of 16,757 bacterial clones will facilitate systematic analyses of the connections among gene sequence, chromosomal location and gene function in C. elegans...
  3. pmc Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity
    James P Hewitson
    Centre for Immunity, Infection and Evolution, Institute of Immunology and Infection Research, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 167:1-11. 2009
    ....
  4. ncbi SMEDWI-2 is a PIWI-like protein that regulates planarian stem cells
    Peter W Reddien
    Howard Hughes Medical Institute, Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Science 310:1327-30. 2005
    ..We suggest that SMEDWI-2 functions within dividing neoblasts to support the generation of cells that promote regeneration and homeostasis...
  5. pmc Beta-catenin defines head versus tail identity during planarian regeneration and homeostasis
    Kyle A Gurley
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, 401 MREB, 20N 1900E, Salt Lake City, UT 84132, USA
    Science 319:323-7. 2008
    ..We suggest that beta-catenin functions as a molecular switch to specify and maintain anteroposterior identity during regeneration and homeostasis in planarians...
  6. pmc Evaluation of urine CCA assays for detection of Schistosoma mansoni infection in Western Kenya
    Hillary L Shane
    Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
    PLoS Negl Trop Dis 5:e951. 2011
    ..Our results suggest that urine-based assays for CCA may be valuable in screening for S. mansoni infections...
  7. pmc The major component in schistosome eggs responsible for conditioning dendritic cells for Th2 polarization is a T2 ribonuclease (omega-1)
    Svenja Steinfelder
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 206:1681-90. 2009
    ..Based on this evidence, we hypothesize that S. mansoni omega-1 acts by limiting the interaction of DCs with CD4(+) T lymphocytes, thereby lowering the strength of the activation signal delivered...
  8. pmc Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses
    Bart Everts
    Department of Parasitology, Leiden University Medical Centre, Leiden 2333 ZA, The Netherlands
    J Exp Med 206:1673-80. 2009
    ..Collectively, we identify omega-1, a single component of SEA, as a potent inducer of Th2 responses...
  9. pmc A wound-induced Wnt expression program controls planarian regeneration polarity
    Christian P Petersen
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:17061-6. 2009
    ..These data indicate that wound-induced input is involved in resetting the normal polarized features of the body axis during regeneration...
  10. pmc Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling
    Sasisekhar Bennuru
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Negl Trop Dis 3:e410. 2009
    ..This proteomic analysis extends our knowledge of the ES and provides insight into the host-parasite interaction...
  11. ncbi Serine protease inhibitors of parasitic helminths
    Adebayo J Molehin
    Molecular Parasitology Laboratory, Department of Biology, Queensland Institute of Medical Research, 300 Herston Road, Herston, Queensland, Australia
    Parasitology 139:681-95. 2012
    ..Clonorchis sinensis, Paragonimus westermani and Echinococcus spp. and discusses their possible biological functions, including roles in host-parasite interplay and their evolutionary relationships...
  12. ncbi Functions of the tegument of schistosomes: clues from the proteome and lipidome
    Jaap J van Hellemond
    Department Biochemistry and Cell Biology, Faculty of Veterinary Medicine and Institute of Biomembranes, Utrecht University, P O Box 80176, 3508 TD Utrecht, The Netherlands
    Int J Parasitol 36:691-9. 2006
    ..The tegumental proteome and lipidome, therefore, imply that many unknown molecular mechanisms are employed by schistosomes to survive within their host...
  13. pmc Platyhelminth Venom Allergen-Like (VAL) proteins: revealing structural diversity, class-specific features and biological associations across the phylum
    Iain W Chalmers
    Institute of Biological, Environmental and Rural Sciences, Edward Llwyd Building, Penglais Campus, Aberystwyth University, Aberystwyth SY23 3DA, UK
    Parasitology 139:1231-45. 2012
    ..Our comprehensive analysis of platyhelminth VALs represents a unifying synopsis for understanding diversity within this protein family and a firm context in which to initiate future functional characterization of these enigmatic members...
  14. ncbi Modulation of macrophage cytokine production by ES-62, a secreted product of the filarial nematode Acanthocheilonema viteae
    H S Goodridge
    Department of Immunology and Bacteriology, University of Glasgow, Western Infirmary, Dumbarton Road, Glasgow G11 6NT, United Kingdom
    J Immunol 167:940-5. 2001
    ..This likely contributes to the generation of immune responses with an anti-inflammatory Th2 phenotype, a well-documented feature of filarial nematode infection...
  15. ncbi Making sense of the schistosome surface
    Patrick J Skelly
    Tufts Cummings School of Veterinary Medicine, Department of Biomedical Sciences, 20 Westboro Road, North Grafton, MA 01536, USA
    Adv Parasitol 63:185-284. 2006
    ..We consider the way in which the tegument surface is formed after cercarial penetration into the skin, and changes that occur as parasites develop to maturity. Lastly, we review the evidence on surface dynamics and turnover...
  16. ncbi Helminth-derived immunomodulators: can understanding the worm produce the pill?
    William Harnett
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
    Nat Rev Immunol 10:278-84. 2010
    ....
  17. ncbi Smed-betacatenin-1 is required for anteroposterior blastema polarity in planarian regeneration
    Christian P Petersen
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 319:327-30. 2008
    ..Our data suggest that beta-catenin specifies the posterior character of the anteroposterior axis throughout the Bilateria and specifies regeneration polarity in planarians...
  18. pmc The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians
    Dasaradhi Palakodeti
    Department of Genetics and Developmental Biology, University of Connecticut Stem Cell Institute, Farmington, Connecticut 06030 3301, USA
    RNA 14:1174-86. 2008
    ..Therefore, SMEDWI-2 and SMEDWI-3 are required for piRNA expression, regeneration, and neoblast function in S. mediterranea...
  19. ncbi The secretome of the filarial parasite, Brugia malayi: proteomic profile of adult excretory-secretory products
    James P Hewitson
    Institute of Immunology and Infection Research, University of Edinburgh, Ashworth Laboratories, Edinburgh, UK
    Mol Biochem Parasitol 160:8-21. 2008
    ....
  20. pmc Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo
    P GONCZY
    European Molecular Biology Laboratory, Heidelberg, D 69117 Germany
    J Cell Biol 147:135-50. 1999
    ..In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation...
  21. pmc Excretory/secretory proteome of the adult developmental stage of human blood fluke, Schistosoma japonicum
    Feng Liu
    double daggerShanghai Ministry of Science and Technology Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, 351 Guo Shou Jing Road, Shanghai 201203, China
    Mol Cell Proteomics 8:1236-51. 2009
    ..japonicum ES proteins, provides an insight into host-parasite interactions, and establishes a resource for the development of diagnostic agents and vaccines for the control of schistosomiasis...
  22. ncbi Parasitism proteins in nematode-plant interactions
    Eric L Davis
    North Carolina State University, Department of Plant Pathology, Raleigh, NC 27695, USA
    Curr Opin Plant Biol 11:360-6. 2008
    ....
  23. pmc Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses
    Jennifer M Fitzpatrick
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS Negl Trop Dis 3:e543. 2009
    ....
  24. pmc An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host
    Mark W Robinson
    Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Ultimo, Sydney, New South Wales 2007, Australia
    Mol Cell Proteomics 8:1891-907. 2009
    ..hepatica are secreted into the parasite gut via a classical endoplasmic reticulum/Golgi pathway, we speculate that the antioxidants, which all lack a signal sequence, are released via a non-classical trans-tegumental pathway...
  25. ncbi An abundant, trans-spliced mRNA from Toxocara canis infective larvae encodes a 26-kDa protein with homology to phosphatidylethanolamine-binding proteins
    D Gems
    Wellcome Research Centre for Parasitic Infections, Department of Biology, Imperial College of Science, Technology and Medicine, London, United Kingdom
    J Biol Chem 270:18517-22. 1995
    ..Assays with the T. canis recombinant 26-kDa protein expressed as a fusion with maltose-binding protein have confirmed phosphatidylethanolamine-binding specificity for this novel product...
  26. ncbi The parasitic helminth product ES-62 suppresses pathogenesis in collagen-induced arthritis by targeting the interleukin-17-producing cellular network at multiple sites
    Miguel A Pineda
    University of Glasgow, Glasgow, UK
    Arthritis Rheum 64:3168-78. 2012
    ..Since interleukin-17 (IL-17) has been reported to play a pathogenic role in the development of RA, this study was undertaken to investigate whether targeting of IL-17 may explain the protection against CIA afforded by ES-62...
  27. pmc Direct identification of the Meloidogyne incognita secretome reveals proteins with host cell reprogramming potential
    Stephane Bellafiore
    Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA
    PLoS Pathog 4:e1000192. 2008
    ..incognita secretome overlaps the reported secretome of mammalian parasitic nematodes (e.g., Brugia malayi), suggesting a common parasitic behavior and a possible conservation of function between metazoan parasites of plants and animals...
  28. ncbi Tetraspanins on the surface of Schistosoma mansoni are protective antigens against schistosomiasis
    Mai H Tran
    Helminth Biology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, 300 Herston Road, Brisbane, Queensland 4006, Australia
    Nat Med 12:835-40. 2006
    ..When coupled with its selective recognition by naturally resistant people, TSP-2 seems to be an effective vaccine antigen against S. mansoni...
  29. ncbi The C. elegans protein EGL-1 is required for programmed cell death and interacts with the Bcl-2-like protein CED-9
    B Conradt
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Cell 93:519-29. 1998
    ..We propose that EGL-1 activates programmed cell death by binding to and directly inhibiting the activity of CED-9, perhaps by releasing the cell death activator CED-4 from a CED-9/CED-4-containing protein complex...
  30. pmc The transcriptome of Trichuris suis--first molecular insights into a parasite with curative properties for key immune diseases of humans
    Cinzia Cantacessi
    Department of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia
    PLoS ONE 6:e23590. 2011
    ..Although it is understood that short-term T. suis infection in people with such diseases usually induces a modified Th2-immune response, nothing is known about the molecules in the parasite that induce this response...
  31. ncbi Proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, Ancylostoma caninum
    Jason Mulvenna
    Helminth Biology Laboratory, Division of Infectious Diseases, Queensland Institute of Medical Research, Brisbane, Queensland 4006, Australia
    Mol Cell Proteomics 8:109-21. 2009
    ..This work identified a suite of proteins important for the parasitic lifestyle and provides new insight into the biology of hookworm infection...
  32. pmc Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family
    Iain W Chalmers
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    BMC Genomics 9:89. 2008
    ..Proteins containing this domain are implicated in diverse biological activities and may be important for chronic host/parasite interactions...
  33. ncbi Proteins exposed at the adult schistosome surface revealed by biotinylation
    Simon Braschi
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    Mol Cell Proteomics 5:347-56. 2006
    ..Collectively the labeled parasite proteins merit investigation as potential vaccine candidates...
  34. pmc Proteomic analysis of excretory-secretory products of Heligmosomoides polygyrus assessed with next-generation sequencing transcriptomic information
    Yovany Moreno
    Institute of Parasitology and Centre for Host Parasite Interactions, McGill University, Ste Anne de Bellevue, Quebec, Canada
    PLoS Negl Trop Dis 5:e1370. 2011
    ..polygyrus biology. In addition, the methods and analyses presented here are applicable to study biochemical and molecular aspects of the host-parasite relationship in species for which sequence information is not available...
  35. ncbi Silencing of Smed-betacatenin1 generates radial-like hypercephalized planarians
    Marta Iglesias
    Departament de Genetica, Universitat de Barcelona, 08028 Barcelona, Spain
    Development 135:1215-21. 2008
    ..mediterranea beta-catenins could be functionally specialized and that only Smed-betacatenin1 is involved in Wnt signalling...
  36. ncbi The FAR proteins of filarial nematodes: secretion, glycosylation and lipid binding characteristics
    Antonio Garofalo
    School of Life and Environmental Sciences, University of Nottingham, University Park, UK
    Mol Biochem Parasitol 122:161-70. 2002
    ....
  37. pmc Schistosoma mansoni tegument protein Sm29 is able to induce a Th1-type of immune response and protection against parasite infection
    Fernanda C Cardoso
    Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    PLoS Negl Trop Dis 2:e308. 2008
    ..In this study, we investigated murine cellular immune responses to recombinant (r) Sm29 and tested this protein as a vaccine candidate...
  38. ncbi Mapping fucosylated epitopes on glycoproteins and glycolipids of Schistosoma mansoni cercariae, adult worms and eggs
    M L M Robijn
    Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands
    Parasitology 130:67-77. 2005
    ..Clearly, the use of defined mAbs provides a quick and convenient way to map expression profiles of carbohydrate epitopes...
  39. pmc RNA interference in Schistosoma mansoni schistosomula: selectivity, sensitivity and operation for larger-scale screening
    Sasa Stefanic
    Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences QB3, University of California San Francisco, San Francisco, California, United States of America
    PLoS Negl Trop Dis 4:e850. 2010
    ..Therefore, in the context of standardizing larger RNAi screens, data are limited on the extent of possible off-targeting effects, gene-to-gene variability in RNAi efficiency and the operational capabilities and limits of RNAi...
  40. ncbi A comparative proteomic study of the undeveloped and developed Schistosoma mansoni egg and its contents: the miracidium, hatch fluid and secretions
    William Mathieson
    Department of Biology, University of York, P O Box 373, York YO105YW, UK
    Int J Parasitol 40:617-28. 2010
    ....
  41. pmc Stage-specific proteomic expression patterns of the human filarial parasite Brugia malayi and its endosymbiont Wolbachia
    Sasisekhar Bennuru
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:9649-54. 2011
    ..Parasite sex- and stage-specific protein expression identified those pathways related to parasite differentiation and demonstrates stage-specific expression by the Bm endosymbiont Wolbachia as well...
  42. ncbi Short interfering RNA-mediated gene silencing in Globodera pallida and Meloidogyne incognita infective stage juveniles
    Johnathan J Dalzell
    Parasitology, School of Biological Sciences, Queen s University Belfast, Belfast BT9 7BL, UK
    Int J Parasitol 40:91-100. 2010
    ..This work presents the first application of such an approach in a whole organism; initial results show promise...
  43. ncbi Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory-secretory products of Clonorchis sinensis adult worms
    Jung Won Ju
    Division of Malaria and Parasitic Diseases, National Institute of Health, Seoul, Korea
    Proteomics 9:3066-78. 2009
    ..Our results illustrate the validity of immuno-proteomic approaches in the identification of serodiagnostic antigens in the parasites...
  44. ncbi A Bruno-like gene is required for stem cell maintenance in planarians
    Tingxia Guo
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, B107 Chemical and Life Science Laboratory, 601 South Goodwin Avenue, Urbana, Illinois 61801, USA
    Dev Cell 11:159-69. 2006
    ..These results suggest that Bruli is required for stem cell maintenance...
  45. pmc Protein variation in blood-dwelling schistosome worms generated by differential splicing of micro-exon gene transcripts
    Ricardo DeMarco
    Department of Biology, University of York, York YO10 5YW, United Kingdom
    Genome Res 20:1112-21. 2010
    ..We believe that our data open a new chapter in the study of immune evasion by schistosomes, and their ability to generate variant proteins could represent a significant obstacle to vaccine development...
  46. pmc DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo
    Akram A Da'Dara
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    Vaccine 26:3617-25. 2008
    ..Indeed, mathematical modeling of SjCTPI-Hsp70 and SjC23-Hsp70 alone and in conjunction with human chemotherapy showed a significant reduction in transmission almost to the point of elimination...
  47. ncbi Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome
    Bas W M van Balkom
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    J Proteome Res 4:958-66. 2005
    ..Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival...
  48. ncbi Use of circulating cathodic antigen (CCA) dipsticks for detection of intestinal and urinary schistosomiasis
    J Russell Stothard
    Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UK
    Acta Trop 97:219-28. 2006
    ..At approximately 2.6 US dollars per dipstick, they are presently too expensive to be cost-effective for wide scale use in disease mapping surveys unless Lot Quality Assurance Sampling (LQAS) strategies are developed...
  49. pmc A comparative chemogenomics strategy to predict potential drug targets in the metazoan pathogen, Schistosoma mansoni
    Conor R Caffrey
    Sandler Center for Basic Research in Parasitic Diseases, California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 4:e4413. 2009
    ..The comparative chemogenomics strategy presented generates a tractable set of S. mansoni proteins for experimental validation as drug targets against this insidious human pathogen...
  50. ncbi Cestode genomics - progress and prospects for advancing basic and applied aspects of flatworm biology
    P D Olson
    Department of Zoology, The Natural History Museum, London, UK
    Parasite Immunol 34:130-50. 2012
    ....
  51. ncbi Proteomic analysis of secretory products from the model gastrointestinal nematode Heligmosomoides polygyrus reveals dominance of venom allergen-like (VAL) proteins
    James P Hewitson
    Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH9 3JT, UK
    J Proteomics 74:1573-94. 2011
    ..Overall, this study shows that the secretions of H. polygyrus closely parallel those of clinically important GI nematodes, confirming the value of this parasite as a model of helminth infection...
  52. pmc Schistosoma mansoni Stomatin like protein-2 is located in the tegument and induces partial protection against challenge infection
    Leonardo P Farias
    Centro de Biotecnologia, Instituto Butantan, Sao Paulo, SP, Brazil
    PLoS Negl Trop Dis 4:e597. 2010
    ..Even though an effective treatment exists, it does not prevent re-infection, and the development of an effective vaccine still remains the most desirable means of control for this disease...
  53. pmc The secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke Opisthorchis viverrini
    Jason Mulvenna
    Division of Infectious Diseases, Queensland Institute of Medical Research, QLD, Australia
    Proteomics 10:1063-78. 2010
    ....
  54. pmc Enzymatic shaving of the tegument surface of live schistosomes for proteomic analysis: a rational approach to select vaccine candidates
    William Castro-Borges
    Department of Biology, University of York, Heslington, York, United Kingdom
    PLoS Negl Trop Dis 5:e993. 2011
    ..Biotinylation of live worms using membrane-impermeant probes revealed that only a small subset of the proteins was accessible to the reagents. Their position within the multilayered architecture of the surface has not been ascertained...
  55. pmc The TALE class homeobox gene Smed-prep defines the anterior compartment for head regeneration
    Daniel A Felix
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, United Kingdom
    PLoS Genet 6:e1000915. 2010
    ..Together our results suggest that Smed-prep is required in stem cell progeny as they form the anterior regenerative blastema and is required for specifying anterior cell fates and correct patterning...
  56. ncbi Comparative analysis of the excretory-secretory proteome of the muscle larva of Trichinella pseudospiralis and Trichinella spiralis
    Mark W Robinson
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK
    Int J Parasitol 37:139-48. 2007
    ..The value of this approach will be further enhanced by data arising out the current T. spiralis genome sequencing project...
  57. ncbi A helminth immunomodulator reduces allergic and inflammatory responses by induction of IL-10-producing macrophages
    Corinna Schnoeller
    Department of Molecular Parasitology, Humboldt University, Berlin, Germany
    J Immunol 180:4265-72. 2008
    ..Our data demonstrate that treatment with a single helminth protein can exert the antiallergic effects of helminth infections...
  58. ncbi Proteomic analysis of excretory/secretory products released by Teladorsagia circumcincta larvae early post-infection
    S K Smith
    Moredun Research Institute, Midlothian, UK
    Parasite Immunol 31:10-9. 2009
    ..ELISA was performed to assess antigen-specific mucus IgA levels in individual sheep. These experiments provided preliminary evidence that the proteins identified in the larval secretome were subject to these antibody responses...
  59. pmc Proteomic analysis of skin invasion by blood fluke larvae
    Elizabeth Hansell
    Sandler Center, California Institute for Quantitative Biosciences QB3, University of California, San Francisco, California, United States of America
    PLoS Negl Trop Dis 2:e262. 2008
    ..To better understand the pathobiology of this initial event in schistosome infection, a proteome analysis of human skin was carried out following invasion by cercariae of Schistosoma mansoni...
  60. pmc Helminth 2-Cys peroxiredoxin drives Th2 responses through a mechanism involving alternatively activated macrophages
    Sheila Donnelly
    Level 6, Bldg 4, University of Technology Sydney, Cnr Thomas and Harris St, Ultimo, Sydney, NSW 2007, Australia
    FASEB J 22:4022-32. 2008
    ..We propose that Prx activates macrophages as an initial step in the induction of Th2 responses by helminth parasites and is thereby a novel pathogen-associated molecular pattern...
  61. ncbi A portrait of the "SCP/TAPS" proteins of eukaryotes--developing a framework for fundamental research and biotechnological outcomes
    C Cantacessi
    Department of Veterinary Science, The University of Melbourne, Australia
    Biotechnol Adv 27:376-88. 2009
    ....
  62. pmc Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases
    Hanns Soblik
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Mol Cell Proteomics 10:M111.010157. 2011
    ..The characterization of stage-related ESP from Strongyloides will help to further understand the interaction of this unique intestinal nematode with its host...
  63. ncbi The phosphorycholine moiety of the filarial nematode immunomodulator ES-62 is responsible for its anti-inflammatory action in arthritis
    M M Harnett
    Division of Immunology, Infection and Inflammation, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, UK
    Ann Rheum Dis 67:518-23. 2008
    ..As a first step to developing ES-62-based drugs, the aim of this study was to determine whether the PC-moiety of ES-62 was responsible for its anti-inflammatory actions...
  64. ncbi Characterisation of the cysteine protease ER60 in transgenic Schistosoma mansoni larvae
    Volker Wippersteg
    Institute for Genetics, Genetic Parasitology and Center for Biological and Medical Research, Heinrich Heine University, D 40225 Dusseldorf, Germany
    Int J Parasitol 32:1219-24. 2002
    ..These results suggest that ER60 is expressed in the ES system of larvae and, amongst other functions, may play a role in penetration and migration processes...
  65. pmc Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction
    Julie E Gleason
    Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA
    Genes Dev 16:1281-90. 2002
    ..These data suggest that hyperactivation of Wnt signaling is sufficient to cause VPCs to adopt induced fates and that a canonical Wnt pathway may play an important role during C. elegans vulval induction...
  66. pmc SmedGD: the Schmidtea mediterranea genome database
    Sofia M C Robb
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Nucleic Acids Res 36:D599-606. 2008
    ..neuro.utah.edu) will prove useful not only to the planarian research community, but also to those engaged in developmental and evolutionary biology, comparative genomics, stem cell research and regeneration...
  67. ncbi Stage-specific alternative splicing of the heat-shock transcription factor during the life-cycle of Schistosoma mansoni
    D Ram
    Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Parasitology 129:587-96. 2004
    ..Yet, they are not regular introns since they are often found in mature mRNA. Alternative splicing of these DNA segments caused structural diversity that could modulate the function of the gene product...
  68. pmc Diagnosis of schistosomiasis by reagent strip test for detection of circulating cathodic antigen
    G J van Dam
    Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    J Clin Microbiol 42:5458-61. 2004
    ..This assay can easily be developed to an end-user format...
  69. ncbi Accuracy of circulating cathodic antigen tests for rapid mapping of Schistosoma mansoni and S. haematobium infections in Southern Sudan
    Ruth A Ashton
    Malaria Consortium Africa Regional Office, Kampala, Uganda
    Trop Med Int Health 16:1099-103. 2011
    ..To evaluate the diagnostic accuracy of a circulating cathodic antigen (CCA) urine dipstick test for detecting Schistosoma mansoni and S. haematobium alongside an integrated rapid mapping survey in Southern Sudan...
  70. ncbi MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegans embryos
    B W Draper
    Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cell 87:205-16. 1996
    ..We propose that MEX-3 contributes to anterior-posterior asymmetry by regulating one or more mRNAs involved in specifying the fate of the posterior blastomere...
  71. pmc Complex patterns of alternative splicing mediate the spatial and temporal distribution of perlecan/UNC-52 in Caenorhabditis elegans
    G P Mullen
    Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4
    Mol Biol Cell 10:3205-21. 1999
    ..Neither short isoforms nor isoforms containing the C-terminal agrin-like region are essential for sarcomere assembly or muscle cell attachment, and their role in development remains unclear...
  72. pmc Heterochromatic deposition of centromeric histone H3-like proteins
    S Henikoff
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109 1024, USA
    Proc Natl Acad Sci U S A 97:716-21. 2000
    ..Rather, the heterochromatic state itself may help localize centromeric components...
  73. ncbi Therapeutic potential of helminth soluble proteins in TNBS-induced colitis in mice
    Nathalie E Ruyssers
    Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, Antwerp, Belgium
    Inflamm Bowel Dis 15:491-500. 2009
    ..We investigated the therapeutic potential of Schistosoma mansoni and Ancylostoma caninum soluble proteins on experimental colitis in mice...
  74. ncbi daf-7-related TGF-beta homologues from Trichostrongyloid nematodes show contrasting life-cycle expression patterns
    H J McSorley
    Centre for Immunity, Infection and Evolution, and Institute for Immunology and Infection Research, Ashworth Laboratories, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland
    Parasitology 137:159-71. 2010
    ..The molecular evolution of this gene family in parasitic nematodes has diversified the pre-protein and life-cycle expression patterns of TGF-beta homologues while conserving the structure of the active domain...
  75. pmc The complete mitochondrial genome sequence of the filarial nematode Wuchereria bancrofti from three geographic isolates provides evidence of complex demographic history
    Akshaya Ramesh
    Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH 44106 4983, USA
    Mol Biochem Parasitol 183:32-41. 2012
    ..We illustrate the utility of this sequence and single nucleotide polymorphisms by inferring the divergence times between the three W. bancrofti isolates, suggesting predictions into their origin and migration...
  76. ncbi Recombinant Ov-ASP-1, a Th1-biased protein adjuvant derived from the helminth Onchocerca volvulus, can directly bind and activate antigen-presenting cells
    Yuxian He
    Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10065, USA
    J Immunol 182:4005-16. 2009
    ....
  77. pmc Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini
    Porntip Pinlaor
    Department of Pathology, Khon Kaen University, Khon Kaen, Thailand
    PLoS Negl Trop Dis 3:e398. 2009
    ..viverrini infection. During its feeding activity within the bile duct, the parasite secretes several cathepsin F cysteine proteases that may induce or contribute to the pathologies associated with hepatobiliary abnormalities...
  78. ncbi Pristionchus pacificus daf-16 is essential for dauer formation but dispensable for mouth form dimorphism
    Akira Ogawa
    Max Planck Institute for Developmental Biology, 72076 Tubingen, Germany
    Development 138:1281-4. 2011
    ....
  79. pmc Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon
    Louis Albert Tchuem Tchuenté
    Centre for Schistosomiasis and Parasitology, Yaounde, Cameroon
    PLoS Negl Trop Dis 6:e1758. 2012
    ..We evaluated the accuracy of circulating cathodic antigen (CCA) urine-assay as a diagnostic tool of S. mansoni. In comparison, a low sensitive CCA test (CCA-L) was assessed...
  80. ncbi On the role of RNA amplification in dsRNA-triggered gene silencing
    T Sijen
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Cell 107:465-76. 2001
    ..This amplification mechanism substantially augments the potency of RNAi-based surveillance, while ensuring that the RNAi machinery will focus on expressed mRNAs...
  81. ncbi Mapping of the complement C9 binding domain in paramyosin of the blood fluke Schistosoma mansoni
    Jiusheng Deng
    Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    Int J Parasitol 37:67-75. 2007
    ..Taken together, this study has mapped the complement regulatory domain in Pmy, capable of binding to C8 and C9 and preventing polyC9 formation, to its C-terminal region...
  82. ncbi Identification and functional characterization of effectors in expressed sequence tags from various life cycle stages of the potato cyst nematode Globodera pallida
    John T Jones
    Plant Pathology Programme, SCRI, Invergowrie, Dundee DD2 5DA, UK
    Mol Plant Pathol 10:815-28. 2009
    ..Our data are therefore consistent with the suggestion that the SPRYSEC proteins suppress host defences, as suggested previously, and that they achieve this through interaction with a range of host targets...
  83. pmc Regeneration and maintenance of the planarian midline is regulated by a slit orthologue
    Francesc Cebria
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, B107 Chemical and Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    Dev Biol 307:394-406. 2007
    ..These results suggest that Smed-slit acts not only as a repulsive cue required for proper midline formation during regeneration but that it may also act to regulate the behavior of neural precursors at the midline in intact planarians...
  84. ncbi A secreted SPRY domain-containing protein (SPRYSEC) from the plant-parasitic nematode Globodera rostochiensis interacts with a CC-NB-LRR protein from a susceptible tomato
    Sajid Rehman
    Laboratory of Nematology, Wageningen University, Binnenhaven 5, 6709PD Wageningen, The Netherlands
    Mol Plant Microbe Interact 22:330-40. 2009
    ..Alternatively, this SPRYSEC may bind to the immune receptor to downregulate its activity...
  85. ncbi Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus
    Benjamin Schlager
    Department for Evolutionary Biology, Max Planck Institute for Developmental Biology, Tubingen, Germany
    Genesis 47:300-4. 2009
    ..We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus...
  86. ncbi Stem cells are differentially regulated during development, regeneration and homeostasis in flatworms
    Katrien De Mulder
    University of Innsbruck, Institute of Zoology and CMBI, Technikerstrasse 25, A 6020 Innsbruck, Austria
    Dev Biol 334:198-212. 2009
    ..Fourth, piwi expression in hatchlings further supports an embryonic formation of the germline in M. lignano. Our findings address new questions in flatworm stem cell research and provide a basis for comparison with higher organisms...
  87. ncbi Field-based evaluation of a reagent strip test for diagnosis of Schistosoma mansoni by detecting circulating cathodic antigen in urine before and after chemotherapy
    Mengistu Legesse
    Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P O Box 1176, Addis Ababa, Ethiopia
    Trans R Soc Trop Med Hyg 101:668-73. 2007
    ..Diagnosis of S. mansoni infection in urine using reagent strips would provide information on the prevalence of the disease, although further study is needed to improve its sensitivity and specificity...
  88. ncbi Spatial and temporal controls target pal-1 blastomere-specification activity to a single blastomere lineage in C. elegans embryos
    C P Hunter
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0554, USA
    Cell 87:217-26. 1996
    ..We propose that the segregation of PIE-1, combined with the temporal decay of SKN-1, targets pal-1 activity to this posterior lineage, thus coupling the regulation of this conserved posterior patterning gene to asymmetric cell cleavages...
  89. ncbi Necrotic cell death in C. elegans requires the function of calreticulin and regulators of Ca(2+) release from the endoplasmic reticulum
    K Xu
    Department of Molecular Biology and Biochemistry, A232, Nelson Biological Laboratories, Rutgers, The State University of New Jersey, 604 Allison Road, Piscataway, NJ 08855, USA
    Neuron 31:957-71. 2001
    ..elegans and suggest that an essential step in the death mechanism is release of ER-based Ca(2+) stores. ER-driven Ca(2+) release has previously been implicated in mammalian necrosis, suggesting necrotic death mechanisms may be conserved...
  90. pmc The novel cyst nematode effector protein 19C07 interacts with the Arabidopsis auxin influx transporter LAX3 to control feeding site development
    Chris Lee
    Division of Plant Sciences, Interdisciplinary Plant Group and Christopher S Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA
    Plant Physiol 155:866-80. 2011
    ..We propose that Hs19C07 most likely increases LAX3-mediated auxin influx and may provide a mechanism for cyst nematodes to modulate auxin flow into root cells, stimulating cell wall hydrolysis for syncytium development...
  91. pmc RNA interference in schistosomes: machinery and methodology
    Greice Krautz-Peterson
    Molecular Helminthology Laboratory, Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University, Cummings School of Veterinary Medicine, Grafton, MA 01536, USA
    Parasitology 137:485-95. 2010
    ..These analyses, and a review of the literature, permit us to derive and present here a draft of potential RNAi pathways in schistosomes...
  92. ncbi The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme
    J Yuan
    Program of Neurosciences, Harvard Medical School, Boston, Massachusetts 02115
    Cell 75:641-52. 1993
    ..We propose that the CED-3 protein acts as a cysteine protease in the initiation of programmed cell death in C. elegans and that cysteine proteases also function in programmed cell death in mammals...
  93. ncbi Single-cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its "index sorting" function for stem cell research
    Tetsutaro Hayashi
    RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    Dev Growth Differ 52:131-44. 2010
    ..This method could be a powerful and straightforward tool for examining the stem cell biology of not only planarians but also other organisms, including vertebrates...
  94. ncbi Can helminths or helminth-derived products be used in humans to prevent or treat allergic diseases?
    Klaus J Erb
    Department of Pulmonary Research, Boehringer Ingelheim Pharma GmbH and Co KG, Biberach a d Riss, 88440, Germany
    Trends Immunol 30:75-82. 2009
    ..Has the time come to treat patients who have allergic diseases or healthy humans who are at risk of developing these diseases with helminths or helminth-derived products? Here, I discuss the pros and cons of such an approach...
  95. pmc A nematode effector protein similar to annexins in host plants
    Nrupali Patel
    Department of Plant Pathology, North Carolina State University, Raleigh, NC 27607, USA
    J Exp Bot 61:235-48. 2010
    ..The combined data suggest that nematode secretion of an Hs4F01 annexin-like effector into host root cells may mimic plant annexin function during the parasitic interaction...
  96. ncbi Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes
    J Y Hsu
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA
    Cell 102:279-91. 2000
    ..Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes...
  97. pmc The fork head transcription factor FKTF-1b from Strongyloides stercoralis restores DAF-16 developmental function to mutant Caenorhabditis elegans
    Holman C Massey
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA
    Int J Parasitol 36:347-52. 2006
    ..This indicates that, unlike FKTF-1b, the S. stercoralis transcription factor FKTF-1a cannot trigger the shift to dauer-specific gene expression in C. elegans...
  98. ncbi Conservation and novelty in the evolution of cell adhesion and extracellular matrix genes
    H Hutter
    Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Science 287:989-94. 2000
    ..A strategy for concerted evolution of new gene families, as well as conservation of adaptive genes, may underlie the differences between heterochromatin and euchromatin...
  99. ncbi Localization of gender-regulated gene expression in the filarial nematode Brugia malayi
    Daojun Jiang
    Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Int J Parasitol 38:503-12. 2008
    ..Thus, ISH provides a means to independently confirm differential expression of genes identified by other methods. In addition, localization patterns provide insight regarding the function of known or novel genes in the parasite...
  100. ncbi Helminth pathogen cathepsin proteases: it's a family affair
    Mark W Robinson
    Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Ultimo, Sydney, NSW, Australia
    Trends Biochem Sci 33:601-8. 2008
    ..The developmentally regulated expression of these proteases correlates with the passage of the parasite through host tissues and its encounters with different host macromolecules...

Research Grants64

  1. Functional Genomics:Complex Biological Systems
    Stuart Kim; Fiscal Year: 2003
    ..The goal of the meeting is to integrate advances in human disease, modeling of genetic networks, quantitative trait mapping, and data mining techniques. ..
  2. Gene networks specifying cell lineages in a polychaete
    Bruce Bowerman; Fiscal Year: 2007
    ..Newly discovered and conserved loci will also be studied in C. elegans and Drosophila. [unreadable] [unreadable]..
  3. Cytokinesis and the Cytoskeleton in C. elegans Embryos
    BRUCE A BOWERMAN; Fiscal Year: 2010
    ..Matthias Peter at the ETH in Zurich, Switzerland. The processes and genes we propose to investigate are relevant to important human diseases, including cancer and neurodegenerative disease. ..
  4. Control of cell polarity in the C. elegans zygote
    Geraldine Seydoux; Fiscal Year: 2005
    ..We anticipate that our studies will provide significant insights into these processes by taking advantage of the experimental attributes of a simple model system...
  5. GENES CONTROLLING DEVELOPMENT OF TISSUE SPECIALIZATION
    ANNA NEWMAN; Fiscal Year: 2003
    ..abstract_text> ..
  6. Developmental Gene Regulation in Schistosoma mansoni
    David Williams; Fiscal Year: 2006
    ..mansoni genome and studies using DNA micro arrays. Furthermore, we will validate the use of SAGE technology to study differential global gene expression in S. mansoni. ..
  7. TRICHINELLOSIS AND REGULATION OF HOST GENE EXPRESSION
    Douglas Jasmer; Fiscal Year: 2001
    ..Understanding these mechanisms may also contribute unique insight on mechanisms of muscle differentiation and cell cycle regulation in mammalian cells. ..
  8. Mechanisms of anti-schistosome artemisinin chemotherapy
    David Williams; Fiscal Year: 2006
    ..mansoni translationally controlled tumor protein. The critical conclusions of this study will direct subsequent future experiments into the molecular mechanism of artemisinin's anti-parasitic activity. ..
  9. 2003/2005 INTERNATIONAL C. ELEGANS MEETINGS
    Geraldine Seydoux; Fiscal Year: 2004
    ..Previous C. elegans meetings have led to the exchange of knowledge, ideas, methods, mutants, and clones and have been vital in fostering the sense of excitement and the collegiality and cooperativity that characterize this field. ..
  10. HTS Inhibitors:Schistosoma Mansoni Peroxiredoxins(RMI)
    David Williams; Fiscal Year: 2005
    ..This will be the first step in the development of novel antischistosome chemotherapies, which are essential for continued public health measures. ..
  11. Mucosal Immune Responses in Hookworm Infection
    Richard Bungiro; Fiscal Year: 2006
    ..These studies are expected to further our understanding of host-parasite immunological interaction and advance hookworm vaccine research through the development of novel immunization techniques. ..
  12. Structural Basis of Novel Hookworm Vaccines
    Oluwatoyin Asojo; Fiscal Year: 2006
    ..56 Angstroms (protein data bank code 1U53). In addition, a 2 PR-1 domain ASP (Na-ASP-1) has been crystallized with data up to 2.7 Angstroms. [unreadable] [unreadable] [unreadable]..
  13. High Throughput Proteome Analysis of Schistosoma mansoni
    David Williams; Fiscal Year: 2005
    ..Completion of this project will provide information on significant protein complements of S. mansoni and also validate the use of MudPIT in future proteomic studies to define a comprehensive view of the S. mansoni proteomes. ..
  14. Tissue Fragility genes in C. elegans
    John Plenefisch; Fiscal Year: 2005
    ..abstract_text> ..
  15. REGULATION OF GENE EXPRESSION DURING ORGANOGENESIS
    Helen Chamberlin; Fiscal Year: 2004
    ..elegans. A third aim is to functionally characterize this second gene to determine whether its product shares in vivo functions with EGL-38, or whether it regulates lin-48 and cdh-3 expression in other organs. ..
  16. Disulfide redox balance in Schistosoma mansoni
    David Williams; Fiscal Year: 2003
    ..Because disulfide redox balance in schistosomes is centered on a single, key enzyme and is fundamentally different from host mechanisms we propose that this pathway is a promising target for novel, rational drug design. ..
  17. Regulatory systems of Drosophila aging
    Marc Tatar; Fiscal Year: 2007
    ..In a comprehensive experimental design, we shall assess qualifies of stress resistance, central metabolism and mitochondria function to determine which feature best predicts variance in life span [unreadable] [unreadable]..
  18. Proteomic Analysis of Hookworm Larval Activation
    John Hawdon; Fiscal Year: 2006
    ..These proteins represent potential vaccine antigens and drug targets for the prevention of hookworm infection and the control of hookworm disease. [unreadable] [unreadable]..
  19. HOOKWORM LARVAL INFECTIVITY AND DEVELOPMENT
    Peter Hotez; Fiscal Year: 2002
    ..The pharmacologic manipulation of parasite-derived protein kinases may offer a new approach to chemotherapy by taking larvae out of arrest. ..
  20. MOLECULAR GENETIC ANALYSIS OF ASYMMETRIC CELL DIVISIONS
    Chris Doe; Fiscal Year: 2002
    ..We will do RNA and protein localization studies; our collaborator, Dr. G. Oliver will generate and assay the gene knock-out mice. ..
  21. GERM LAYER SPECIFICATION
    DAVID MC CLAY; Fiscal Year: 2007
    ..This network eventually will enable us to explore how positional cues are produced and displayed for later morphogenetic patterning. ..
  22. Function of a Neuropeptide Gene Family in C. elegans
    Christine Li; Fiscal Year: 2006
    ..In addition, because C. elegans is the current genetic model system for parasitic nematodes, elucidating the functions of neuropeptides and their signaling pathways may identify new antihelmintic drug targets. ..
  23. MOLECULAR MECHANISMS OF GRANULE CELL MIGRATION
    MARY HATTEN; Fiscal Year: 2007
    ..This information should broaden our view of the mechanisms that control CNS migrations and of the downstream events important to neuronal differentiation within the neural layers. ..
  24. Training in Synaptic Neurobiology
    James Bamburg; Fiscal Year: 2006
    ....
  25. Gustatory and olfactory receptors of Anopheles gambiae
    Hugh Robertson; Fiscal Year: 2006
    ..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. [unreadable] [unreadable]..
  26. Embryonic Development of the Cerebellum
    MARY HATTEN; Fiscal Year: 2007
    ..abstract_text> ..
  27. MECHANISMS IN THE REGULATION OF APOPTOSIS
    Jerry Adams; Fiscal Year: 2006
    ..Justification: As the proposal addresses central issues about the regulation of apoptosis, builds onproductive research, and exploits novel findings, it should significantly advance the molecular dissection of apoptosis. ..
  28. ICSBP Function During Myeloid Differentiation
    Elizabeth Eklund; Fiscal Year: 2006
    ..These studies will determine if ICSBP tyrosine phosphorylation is necessary for myeloid differentiation and leukemia suppression, providing a unique model to dissect early events in the evolution of myeloid malignancy. ..
  29. GENETICS OF CEREBELLAR AXON FORMATION
    MARY HATTEN; Fiscal Year: 2004
    ..In the BAC transgenic animals we can examine the effect of kinase-deficient neurons on the development of the other cerebellar neurons and glia. ..
  30. HoxA10 Function During Myeloid Differentiation
    Elizabeth Eklund; Fiscal Year: 2006
    ..These investigations will identify the molecular mechanisms of target gene regulation by HoxA10, which may suggest a role for HoxA10 in differentiation block in myeloid leukemia. ..
  31. Function and pharmacology of schistosome multidrug resistance proteins
    Robert M Greenberg; Fiscal Year: 2010
    ..We propose to use several approaches to determine the properties of one such schistosome molecule, P-glycoprotein, which, in vertebrates, is also involved in resistance to a broad array of drugs. ..
  32. Genetic and Biochemical Analysis of SID-1and SID-2
    Craig Hunter; Fiscal Year: 2008
    ..4). To isolate and characterize endogenous extracellular RNAs in C. elegans. This work may identify an ancient, gene-specific mechanism for intercellular communication in animals. ..
  33. New Perspectives in Transporter Biology-FASEB conference
    Robert Edwards; Fiscal Year: 2003
    ..Each oral session will be followed by a panel discussion of the topic. To promote the advancement of junior faculty, the most exciting poster submissions will be selected for short oral presentations during the regular oral sessions. ..
  34. ROLE OF ADF PHOSPHORYLATION IN NEURONAL DEVELOPMENT
    James Bamburg; Fiscal Year: 2003
    ..These studies will advance our understanding of nerve growth cone guidance and the design of agents that allow neurons to grow and regenerated in a normally non-permissive environment. ..
  35. Zebrafish in Comparative Context: A Symposium
    Thomas Schilling; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  36. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
  37. Hypoxia-Inducible Factor 1 and Oxygen Homeostasis
    Gregg Semenza; Fiscal Year: 2009
    ....
  38. Functional genomics of molluscan schistosome development
    TIMOTHY YOSHINO; Fiscal Year: 2009
    ..Results of this work may lead to novel strategies for controlling infections within the snail host by disrupting specific larval development-dependent genes or their products. ..
  39. Involvement of a K+ Channel in C. elegans Male Behavior
    ELIZABETH DESTASIO; Fiscal Year: 2005
    ..Lastly, this research will help delineate the connections between motility, functions of particular muscle cells, and organismal behavior. ..
  40. Asymmetric cell division in the C elegans embryo
    Robert Goldstein; Fiscal Year: 2007
    ..We expect that we will learn important new information about how cells divide asymmetrically that will suggest mechanisms and guide studies in mammals, including humans. ..
  41. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
  42. Genetic and Biochemical Analysis of SID-1 and SID-2
    Craig Hunter; Fiscal Year: 2007
    ..Although RNAi has been shown to be effective, a major obstacle remains delivery of dsRNA into human cells both in culture and in vivo. ..
  43. Function of the protein EFF-1 in cell membrane fusion
    William Mohler; Fiscal Year: 2007
    ..Our results will increase understanding of the molecular mechanism underlying developmental cell fusion, a crucial and under-explored component of animal reproduction, development, and health. ..
  44. MARINER TRANSPOSONS OF MAMMALS
    Hugh Robertson; Fiscal Year: 2001
    ..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
  45. Retinoid Signaling in Neural Patterning in Zebrafish
    Thomas Schilling; Fiscal Year: 2009
    ....
  46. Gene Duplication and Genome Evolution
    Austin Hughes; Fiscal Year: 2004
    ..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
  47. IMMUNOREGULATION IN SCHISTOSOMIASIS
    Miguel Stadecker; Fiscal Year: 2005
    ..The long-term goal of this project is the achievement of effective and lasting specific T cell tolerance by way of a suitable biological vector capable of delivering the peptides to the host suffering from, or susceptible to, disease. ..
  48. Mes-1 and Asymmetric Germline Divisions in C. elegans
    LAURA BERKOWITZ; Fiscal Year: 2003
    ..Identification of other genes that function in this process will not only lay the foundation for future experiments but will also advance our understanding of asymmetric divisions. ..
  49. NEURAL TUBE DEFECTS
    DAVID MC CLAY; Fiscal Year: 2005
    ..abstract_text> ..
  50. IMMUNE PEPTIDES IN AEDES AEGYPTI
    TIMOTHY YOSHINO; Fiscal Year: 2002
    ....
  51. Sea Urchin Developmental Biology Conference XIV
    DAVID MC CLAY; Fiscal Year: 2002
    ..The concurrent sessions offer valuable opportunities for graduate students and postdocs to gain experience speaking at an international meeting, and the workshops introduce new approaches in these embryos. ..
  52. Molecular Genetics of Hemicentin
    Bruce Vogel; Fiscal Year: 2006
    ....
  53. Analysis of endocytosis in Caenorhabditis elegans
    Hanna Fares; Fiscal Year: 2006
    ..New genes identified in worms will also be studied in humans through collaborations with researchers who study this process. ..
  54. INDYs: A Link between Membrane Transport and Life Span
    Vadivel Ganapathy; Fiscal Year: 2006
    ..abstract_text> ..
  55. Developmental Genomics in C. elegans
    Craig Hunter; Fiscal Year: 2006
    ..This analysis, although limited to those genes that are transcriptional targets of pal-1 activity, is the necessary first step to a complete description of the activity of a master regulator. ..
  56. NIMH COR HONORS UNDERGRADUATE RESEARCH TRAINING
    STEPHEN PRYOR; Fiscal Year: 2008
    ..abstract_text> ..
  57. Modular design of central metabolism in methylotrophs
    Gregory Crowther; Fiscal Year: 2006
    ..abstract_text> ..
  58. Rationally-Designed Synthetic Antithrombin Activators
    Umesh Desai; Fiscal Year: 2005
    ..The proposed work is critical for determining the organic activator to focus advanced phase II efforts. ..
  59. Designing Mechanism-based Anticoagulants
    Umesh Desai; Fiscal Year: 2006
    ..abstract_text> ..
  60. Conference--Molecular Helminthology, Integrated Approach
    Philip Loverde; Fiscal Year: 2005
    ..Certainly there are fascinating scientific questions waiting to be answered about helminths. Some of those questions have as their central theme the very nature of parasitism. ..
  61. HOST GENETIC CORRELATES IN SCHISTOSOMIASIS
    Philip Loverde; Fiscal Year: 2004
    ..We expect to improve our understanding of the underlying genetic determinants of resistance to reinfection with schistosomiasis and disease progression. ..
  62. The molecular basis of planarian regeneration
    ALEJANDRO SANCHEZ ALVARADO; Fiscal Year: 2006
    ..abstract_text> ..
  63. SIGNAL TRANSDUCTION IN SCHISTOSOMES
    Philip Loverde; Fiscal Year: 2004
    ..We expect to identify useful targets for control of parasite development and/or parasite caused morbidity. ..