chaperonin 60

Summary

Summary: Members of the chaperonin heat-shock protein family. Chaperonin 60 purified from bacteria, plastids, or mitochondria is an oligomeric protein with a distinctive structure of fourteen subunits, arranged in two rings of seven subunits each.

Top Publications

  1. ncbi Two families of chaperonin: physiology and mechanism
    Arthur L Horwich
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Annu Rev Cell Dev Biol 23:115-45. 2007
  2. ncbi The Hsp70 and Hsp60 chaperone machines
    B Bukau
    Institut für Biochemie and Molekularbiologie, Universitat Freiburg, Germany
    Cell 92:351-66. 1998
  3. ncbi Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli
    Michael J Kerner
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 122:209-20. 2005
  4. ncbi Heat-shock proteins induce T-cell regulation of chronic inflammation
    Willem van Eden
    Division of Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
    Nat Rev Immunol 5:318-30. 2005
  5. pmc Allosteric transitions of supramolecular systems explored by network models: application to chaperonin GroEL
    Zheng Yang
    Department of Computational Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS Comput Biol 5:e1000360. 2009
  6. ncbi Allostery wiring diagrams in the transitions that drive the GroEL reaction cycle
    RIINA TEHVER
    Biophysics Program, Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA
    J Mol Biol 387:390-406. 2009
  7. ncbi The crystal structure of the bacterial chaperonin GroEL at 2.8 A
    K Braig
    Department of Genetics, Yale University School of Medicine, Boyer Center, New Haven, Connecticut 06510
    Nature 371:578-86. 1994
  8. ncbi Extracellular heat shock proteins in cell signaling
    Stuart K Calderwood
    Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    FEBS Lett 581:3689-94. 2007
  9. ncbi Heat shock protein 60: regulatory role on innate immune cells
    C Habich
    German Diabetes Clinic, German Diabetes Center, Leibniz Institute at the Heinrich Heine University of Düsseldorf, 40225 Dusseldorf, Germany
    Cell Mol Life Sci 64:742-51. 2007
  10. pmc GroEL dependency affects codon usage--support for a critical role of misfolding in gene evolution
    Tobias Warnecke
    Department of Biology and Biochemistry, University of Bath, Bath, UK
    Mol Syst Biol 6:340. 2010

Detail Information

Publications304 found, 100 shown here

  1. ncbi Two families of chaperonin: physiology and mechanism
    Arthur L Horwich
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Annu Rev Cell Dev Biol 23:115-45. 2007
    ..What are the determinants for substrate binding by the type II chaperonins? And is the encapsulated chaperonin cavity a passive container that prevents aggregation, or could it be playing an active role in polypeptide folding?..
  2. ncbi The Hsp70 and Hsp60 chaperone machines
    B Bukau
    Institut für Biochemie and Molekularbiologie, Universitat Freiburg, Germany
    Cell 92:351-66. 1998
  3. ncbi Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli
    Michael J Kerner
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 122:209-20. 2005
    ..We suggest that the chaperonin system may have facilitated the evolution of this fold into a versatile platform for the implementation of numerous enzymatic functions...
  4. ncbi Heat-shock proteins induce T-cell regulation of chronic inflammation
    Willem van Eden
    Division of Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
    Nat Rev Immunol 5:318-30. 2005
    ..In this Review, we discuss the unique characteristics of HSPs that endow them with these immunoregulatory qualities...
  5. pmc Allosteric transitions of supramolecular systems explored by network models: application to chaperonin GroEL
    Zheng Yang
    Department of Computational Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS Comput Biol 5:e1000360. 2009
    ..An important outcome is the assessment of the critical inter-residue interactions formed/broken near the transition state(s), most of which involve conserved residues...
  6. ncbi Allostery wiring diagrams in the transitions that drive the GroEL reaction cycle
    RIINA TEHVER
    Biophysics Program, Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA
    J Mol Biol 387:390-406. 2009
    ..We find that several substrate protein binding residues as well as sites related to ATPase activity belong to a single functional network in GroEL. For GroES, the mobile loop residues and GroES/GroES interface residues are linked...
  7. ncbi The crystal structure of the bacterial chaperonin GroEL at 2.8 A
    K Braig
    Department of Genetics, Yale University School of Medicine, Boyer Center, New Haven, Connecticut 06510
    Nature 371:578-86. 1994
    ..The three-dimensional structure places most of the mutationally defined functional sites on the channel walls and its outward invaginations, and at the ends of the cylinder...
  8. ncbi Extracellular heat shock proteins in cell signaling
    Stuart K Calderwood
    Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    FEBS Lett 581:3689-94. 2007
    ..The possession of a wide range of receptors for the Hsp and Grp family permits binding to a diverse range of cells and the performance of complex multicellular functions particularly in immune cells and neurones...
  9. ncbi Heat shock protein 60: regulatory role on innate immune cells
    C Habich
    German Diabetes Clinic, German Diabetes Center, Leibniz Institute at the Heinrich Heine University of Düsseldorf, 40225 Dusseldorf, Germany
    Cell Mol Life Sci 64:742-51. 2007
    ..Because of its immunoregulatory properties, Hsp60 has been proposed to act as intercellular danger signal, controlling innate and adaptive immune reactions...
  10. pmc GroEL dependency affects codon usage--support for a critical role of misfolding in gene evolution
    Tobias Warnecke
    Department of Biology and Biochemistry, University of Bath, Bath, UK
    Mol Syst Biol 6:340. 2010
    ..These findings provide independent evidence for a role of misfolding in shaping gene evolution and highlight the need to co-characterize adaptations in cis and trans to unravel the workings of integrated molecular systems...
  11. ncbi Homologous plant and bacterial proteins chaperone oligomeric protein assembly
    S M Hemmingsen
    Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada
    Nature 333:330-4. 1988
    ..Chaperonins comprise a class of molecular chaperones that are found in chloroplasts, mitochondria and prokaryotes. Assisted post-translational assembly of oligomeric protein structures is emerging as a general cellular phenomenon...
  12. pmc Proportions of Mycobacterium massiliense and Mycobacterium bolletii strains among Korean Mycobacterium chelonae-Mycobacterium abscessus group isolates
    Hee Youn Kim
    Department of Microbiology, Cancer Research Institute, SNUMRC, Seoul National University College of Medicine, and Seoul National University Hospital, Seoul 110 799, Republic of Korea
    J Clin Microbiol 46:3384-90. 2008
    ..M. massiliense strains that were highly resistant to clarithromycin had a point mutation at the adenine at position 2058 (A(2058)) or 2059 (A(2059)) in the peptidyltransferase region of the 23S rRNA gene...
  13. pmc Anticancer drugs cause release of exosomes with heat shock proteins from human hepatocellular carcinoma cells that elicit effective natural killer cell antitumor responses in vitro
    Li Hong Lv
    Department of Hepatobiliary Surgery, Sun Yat Sen Memorial Hospital, Sun Yat Sen University, 107W Yanjiang Road, Guangzhou 510120, China
    J Biol Chem 287:15874-85. 2012
    ....
  14. pmc GroEL/GroES cycling: ATP binds to an open ring before substrate protein favoring protein binding and production of the native state
    Navneet K Tyagi
    Howard Hughes Medical Institute, Yale School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 106:20264-9. 2009
    ..We conclude that the physiological order, ATP binding before polypeptide, enables more efficient capture of nonnative substrate proteins, and thus allows greater recovery of the native state for any given round of the chaperonin cycle...
  15. pmc On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60
    Custer C Deocaris
    National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    Cell Stress Chaperones 11:116-28. 2006
    ..An understanding of their functional biology may lead to novel designing and development of therapeutic strategies for cancer and aging...
  16. ncbi Heat shock protein 60: identification of specific epitopes for binding to primary macrophages
    Christiane Habich
    German Diabetes Clinic, German Diabetes Center, Leibniz Institute, Heinrich Heine University of Dusseldorf, D 40225 Dusseldorf, Germany
    FEBS Lett 580:115-20. 2006
    ..Analysis of different pro- and eukaryotic HSP60 species indicated that phylogenetically separate HSP60 species use different binding sites on primary macrophages...
  17. ncbi Human heat shock protein 60 induces maturation of dendritic cells versus a Th1-promoting phenotype
    Stefanie B Flohé
    German Diabetes Research Institute, University of Dusseldorf, Dusseldorf, Germany
    J Immunol 170:2340-8. 2003
    ..Thus, HSP60 stimulates DC more rapidly than LPS and elicits a Th1-promoting phenotype. These results suggest that DC play a pivotal role in priming for destructive Th1-type responses at sites of local HSP60 release...
  18. ncbi Exploring the structural dynamics of the E.coli chaperonin GroEL using translation-libration-screw crystallographic refinement of intermediate states
    Charu Chaudhry
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Mol Biol 342:229-45. 2004
    ....
  19. pmc The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures
    O Fayet
    Centre de Recherche de Biochimie et Genetique Cellulaires, Centre National de Recherche Scientifique, Toulouse, France
    J Bacteriol 171:1379-85. 1989
    ..coli growth and physiology...
  20. ncbi Immunoelectron microscopic localization of the 60-kDa heat shock chaperonin protein (Hsp60) in mammalian cells
    B J Soltys
    Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
    Exp Cell Res 222:16-27. 1996
    ..These findings raise interesting questions concerning the possible role of Hsp60 at extramitochondrial sites...
  21. pmc Hsp60 is actively secreted by human tumor cells
    Anna M Merendino
    Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Universita degli Studi di Palermo, Palermo, Italy
    PLoS ONE 5:e9247. 2010
    ..These questions are particularly relevant for cancer biology since it is believed that extracellular chaperones, like Hsp70, may play an active role in tumor growth and dissemination...
  22. pmc Mycobacterium avium in the postgenomic era
    Christine Y Turenne
    McGill University Health Centre, A5 156, 1650 Cedar Avenue, Montreal H3G 1A4, Canada
    Clin Microbiol Rev 20:205-29. 2007
    ..avium subsets in isolation, it is expected that attention to the similarities and differences between M. avium organisms will provide greater insight into their fundamental differences, including their propensity to cause disease...
  23. pmc Physiologic effects of forced down-regulation of dnaK and groEL expression in Streptococcus mutans
    JOSE A LEMOS
    Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL 32610 0424, USA
    J Bacteriol 189:1582-8. 2007
    ....
  24. pmc Expansion and compression of a protein folding intermediate by GroEL
    Zong Lin
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Cell 16:23-34. 2004
    ....
  25. ncbi Immunoepidemiologic profile of Chlamydia trachomatis infection: importance of heat-shock protein 60 and interferon- gamma
    Craig R Cohen
    Department of Obstetrics, Gynecology, and Reproductive Science, University of California, San Francisco, San Francisco, California 94105, USA
    J Infect Dis 192:591-9. 2005
    ..This investigation supports a direct role for C. trachomatis-specific immune responses in altering the risk of infection and suggests immune correlates of protection that are potentially useful in vaccine development...
  26. ncbi Ultrastructure and phylogenetic analysis of 'Candidatus Neoehrlichia mikurensis' in the family Anaplasmataceae, isolated from wild rats and found in Ixodes ovatus ticks
    Makoto Kawahara
    Nagoya City Public Health Research Institute, Hagiyama cho, Mizuho ku, Nagoya, 467 8615, Japan
    Int J Syst Evol Microbiol 54:1837-43. 2004
    ..It is proposed that this new cluster of bacteria should be designated 'Candidatus Neoehrlichia mikurensis'...
  27. pmc Class B scavenger receptor types I and II and CD36 mediate bacterial recognition and proinflammatory signaling induced by Escherichia coli, lipopolysaccharide, and cytosolic chaperonin 60
    Irina N Baranova
    Department of Laboratory Medicine, Clinical Center, National Institute of Diabetes and Digestive Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 188:1371-80. 2012
    ..CD36, mediate recognition of bacteria not only through interaction with cell wall LPS but also with cytosolic chaperonin 60. HeLa cells stably transfected with any of these SR-Bs demonstrated markedly (3- to 5-fold) increased binding ..
  28. pmc In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins
    B A Kaufman
    Department of Molecular Biology, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 97:7772-7. 2000
    ..These data identify bifunctional proteins that participate in the stability of rho(+) mtDNA...
  29. ncbi Cytosolic accumulation of HSP60 during apoptosis with or without apparent mitochondrial release: evidence that its pro-apoptotic or pro-survival functions involve differential interactions with caspase-3
    Dhyan Chandra
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 282:31289-301. 2007
    ....
  30. pmc Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis
    C D Paddock
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 35:2496-502. 1997
    ..chaffeensis...
  31. pmc The autoimmune concept of atherosclerosis
    Cecilia Grundtman
    Laboratory of Autoimmunity, Division for Experimental Pathophysiology and Immunology, Biocenter, Innsbruck Medical University, Innsbruck, Austria
    Curr Opin Lipidol 22:327-34. 2011
    ..Special emphasis is put on oxidized low-density lipoproteins as early endothelial stressors...
  32. ncbi Potentially pathogenic nontuberculous mycobacteria found in aquatic systems. Analysis from a reclaimed water and water distribution system in Mexico City
    A I Castillo-Rodal
    Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico UNAM, Ciudad Universitaria, 04510, Mexico City, DF, Mexico
    Eur J Clin Microbiol Infect Dis 31:683-94. 2012
    ..Two of the identified species found in the reclaimed water, M. avium and M. fortuitum, are considered important human opportunistic pathogens...
  33. ncbi GroEL: More than Just a folding cage
    Sheena E Radford
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom
    Cell 125:831-3. 2006
    ..In this issue, Tang et al. (2006) redesign the GroEL central cavity and show that the chaperonin cage can alter the rate of folding and, for some proteins, could even alter the folding mechanism...
  34. ncbi Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy
    R W Wilson
    Center for Pulmonary and Infectious Disease Control, The University of Texas Health Center, Tyler 75708, USA
    Int J Syst Evol Microbiol 51:1751-64. 2001
    ..By DNA-DNA hybridization, this new group exhibited <30% homology with recognized RGM species. The name Mycobacterium immunogenum sp. nov. is proposed for this new taxon...
  35. pmc Development and application of multiprobe real-time PCR method targeting the hsp65 gene for differentiation of Mycobacterium species from isolates and sputum specimens
    Kijeong Kim
    Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Seoul 110 799, Republic of Korea
    J Clin Microbiol 48:3073-80. 2010
    ..Our data suggest that this novel HMPRT-PCR method could be a promising approach for detecting pathogenic mycobacterial species from sputum samples and culture isolates routinely in a clinical setting...
  36. ncbi NMR analysis of a 900K GroEL GroES complex
    Jocelyne Fiaux
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
    Nature 418:207-11. 2002
    ..This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes...
  37. ncbi Routine use of a simple low-cost genotypic assay for the identification of mycobacteria in a high throughput laboratory
    Derek A Wong
    TB Reference Laboratory, Pathology Service, Department of Health, Hong Kong, China
    Diagn Microbiol Infect Dis 47:421-6. 2003
    ..chelonae, and M. kansasii. It had proven to be highly robust and stable despite usage on such a large-scale and is thus particularly suitable for use in high throughput laboratories in areas with a high incidence of tuberculosis...
  38. ncbi Pyrosequencing of chaperonin-60 (cpn60) amplicons as a means of determining microbial community composition
    John Schellenberg
    Agriculture and Agri Food Canada Saskatoon Research Centre, Saskatoon, SK, Canada
    Methods Mol Biol 733:143-58. 2011
    ..We describe here the methods we have developed for determining microbial community profiles by pyrosequencing of cpn60 UT amplicons, from generating amplicons to sequencing and data analysis...
  39. pmc Chlamydia pneumoniae GroEL1 protein is cell surface associated and required for infection of HEp-2 cells
    Frederik N Wuppermann
    Funktionelle Genomforschung der Mikroorganismen, Heinrich Heine Universitat Dusseldorf, Universitatsstr 1, Geb 25 02 U1, 40225 Dusseldorf, Germany
    J Bacteriol 190:3757-67. 2008
    ..pneumoniae, although adhesion of infectious bacteria to eukaryotic cells was not affected. Taken together, these data support a role for extracellular GroEL1 in the establishment of the chlamydial infection...
  40. ncbi PCR restriction fragment length polymorphism analysis (PRA)-algorithm targeting 644 bp Heat Shock Protein 65 (hsp65) gene for differentiation of Mycobacterium spp
    Hong Kim
    Department of Microbiology and Liver Research Institute, College of Medicine, Seoul National University, 28 Yongon Dong, Chongno Gu, Seoul 110 799, South Korea
    J Microbiol Methods 62:199-209. 2005
    ..Our results suggest that this novel PRA offers a simple, rapid, and accurate method for the identification of mycobacteria culture isolates at the species level...
  41. pmc Interplay of structure and disorder in cochaperonin mobile loops
    S J Landry
    Department of Biochemistry, Tulane University School of Medicine, New Orleans, LA 70112 2699, USA
    Proc Natl Acad Sci U S A 93:11622-7. 1996
    ..Thermodynamic considerations suggest that flexible disorder in the cochaperonin mobile loops moderates their affinity for GroEL to facilitate cycles of chaperonin-mediated protein folding...
  42. pmc Heat-shock protein 60 kDa and atherogenic dyslipidemia in patients with untreated mild periodontitis: a pilot study
    Manfredi Rizzo
    Department of Internal Medicine and Medical Specialties, University of Palermo, Palermo, Italy
    Cell Stress Chaperones 17:399-407. 2012
    ..Thus, the road is open to investigate the potential value of elevated levels of circulating Hsp60 as predictor of risk for cardiovascular disease when associated to dyslipidemia in periodontitis patients...
  43. pmc Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60)
    Joost A Aalberse
    Department of Pediatric Immunology, Wilhelmina Children s Hospital, Utrecht, The Netherlands
    PLoS ONE 6:e24119. 2011
    ..It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have...
  44. ncbi CD30 discriminates heat shock protein 60-induced FOXP3+ CD4+ T cells with a regulatory phenotype
    Isme de Kleer
    Center for Molecular and Cellular Immunology, University Medical Center Utrecht, The Netherlands
    J Immunol 185:2071-9. 2010
    ..These findings have implications for the generation and isolation of pure populations of Ag-specific Tregs, with the potential to prevent and treat human inflammatory diseases...
  45. pmc Expression of heat shock protein 60 kDa is upregulated in cervical cancer
    You Jin Hwang
    Division of Biological Science, Gachon University of Medicine and Science, 534 2 Yeonsu dong, Yeonsu gu, Incheon, Korea
    Yonsei Med J 50:399-406. 2009
    ..In order to evaluate the prognostic significance of HSP60 in cervical cancer, we examined differences in the HSP60 expression between cervical cancer and normal tissues in women...
  46. pmc Exit mechanisms of the intracellular bacterium Ehrlichia
    Sunil Thomas
    Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, United States of America
    PLoS ONE 5:e15775. 2010
    ..Ehrlichia muris and Ixodes ovatus Ehrlichia (IOE) are agents of mouse models of ehrlichiosis. The mechanism by which Ehrlichia are transported from an infected host cell to a non-infected cell has not been demonstrated...
  47. ncbi Principal component and normal mode analysis of proteins; a quantitative comparison using the GroEL subunit
    Lars Skjaerven
    Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N 5009 Bergen, Norway
    Proteins 79:232-43. 2011
    ..The results we obtain on the GroEL subunit contribute to the generalization of robust and reproducible strategies for the study of protein dynamics, using either NMA or PCA of trajectories from MD simulations...
  48. ncbi Mitochondrial Hsp60, resistance to oxidative stress, and the labile iron pool are closely connected in Saccharomyces cerevisiae
    Elisa Cabiscol
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Spain
    J Biol Chem 277:44531-8. 2002
    ..These results indicate that the role of Hsp60 in oxidative-stress defense is explained by protection of several Fe/S proteins, which prevent the release of iron ions and thereby avert further damage...
  49. pmc Facilitated oligomerization of mycobacterial GroEL: evidence for phosphorylation-mediated oligomerization
    C M Santosh Kumar
    Laboratory of Structural Biology, Hyderabad 500001, India
    J Bacteriol 191:6525-38. 2009
    ..tuberculosis GroEL1 revealed that the tetradecameric form is phosphorylated on serine-393, while the heptameric form is not, indicating that the switch between the single- and double-ring variants is mediated by phosphorylation...
  50. pmc Listeria monocytogenes uses Listeria adhesion protein (LAP) to promote bacterial transepithelial translocation and induces expression of LAP receptor Hsp60
    Kristin M Burkholder
    Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907 2009, USA
    Infect Immun 78:5062-73. 2010
    ..These data provide insight into the role of LAP as a virulence factor during intestinal epithelial infection and pose new questions regarding the dynamics between the host stress response and pathogen infection...
  51. ncbi Hydrophilic residues 526 KNDAAD 531 in the flexible C-terminal region of the chaperonin GroEL are critical for substrate protein folding within the central cavity
    Kodai Machida
    Department of Biotechnology, Faculty of Engineering, Graduate School of Medical Science, Tottori University, Koyama Minami, Tottori 680 8552, Japan
    J Biol Chem 283:6886-96. 2008
    ..These results highlight the importance of the hydrophilic nature of 526 KNDAAD 531 residues in the flexible C-terminal region for proper protein folding within the central cavity of GroEL...
  52. ncbi Novel prophylactic and therapeutic vaccine against tuberculosis
    Masaji Okada
    Clinical Research Center, National Hospital Organization Kinki chuo Chest Medical Center, 1180 Nagasone, Kitaku, Sakai, Osaka 591 8555, Japan
    Vaccine 27:3267-70. 2009
    ..These data indicate that our novel DNA vaccine might be useful against Mycobacterium tuberculosis including XDR-TB and MDR-TB for human therapeutic clinical trials...
  53. ncbi The role of streptococcal hypersensitivity in the pathogenesis of Behçet's Disease
    Fumio Kaneko
    Institute of Dermato Immunology and Allergy, Southern Tohoku Research Institute for Neuroscience, Koriyama, Fukushima, Japan
    Eur J Dermatol 18:489-98. 2008
    ..The BD symptoms were thought to be due to vascular reactions as immune responses in correlation with monocyte expressed streptococcal agents...
  54. ncbi Combined and individual mitochondrial HSP60 and HSP10 expression in cardiac myocytes protects mitochondrial function and prevents apoptotic cell deaths induced by simulated ischemia-reoxygenation
    K M Lin
    Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0618, USA
    Circulation 103:1787-92. 2001
    ....
  55. pmc Myxococcus xanthus viability depends on groEL supplied by either of two genes, but the paralogs have different functions during heat shock, predation, and development
    Jian Li
    State Key Laboratory of Microbial Technology, College of Life Science, Shandong University, Jinan, China
    J Bacteriol 192:1875-81. 2010
    ..Our results suggest that, although alternatively required for cell viability, the products of the two groEL genes have divergent functions in the multicellular social life cycle of M. xanthus DK1622...
  56. ncbi Structural basis for GroEL-assisted protein folding from the crystal structure of (GroEL-KMgATP)14 at 2.0A resolution
    J Wang
    Department of Molecular Biophysics and Biochemistry Yale University, 266 Whitney Avenue, Bass Center, Room 418, New Haven, CT 06520 8114, USA
    J Mol Biol 327:843-55. 2003
    ..These results provide a basis for the nucleotide-dependent regulation of protein substrate binding and suggest a mechanism for GroEL-assisted protein folding by forced unfolding...
  57. pmc Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres
    Tim J Dumonceaux
    National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington St, Winnipeg, MB, Canada
    J Clin Microbiol 47:4067-77. 2009
    ..Using the presence of A. vaginae or G. vaginalis, or both, as the defining criterion for BV, we found that the method was highly specific and sensitive for the diagnosis of BV using microscopy as a gold standard...
  58. ncbi The 2.4 A crystal structure of the bacterial chaperonin GroEL complexed with ATP gamma S
    D C Boisvert
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Nat Struct Biol 3:170-7. 1996
    ..Although bound ATP induces modest conformational shifts in the equatorial domain, the stereochemistry that functionally coordinates GroEL's affinity for nucleotides, polypeptide, and GroES remains uncertain...
  59. pmc Extracellular heat shock protein 60, cardiac myocytes, and apoptosis
    Se Chan Kim
    Molecular and Cellular Cardiology, University of California Davis, One Shields Ave, Davis, CA 95616, USA
    Circ Res 105:1186-95. 2009
    ..Previously, we have found that changes in the location of intracellular heat shock protein (HSP)60 are associated with apoptosis. HSP60 has been reported to be a ligand of toll-like receptor (TLR)-4...
  60. ncbi Cardiovascular risk factors and atherosclerosis in young males: ARMY study (Atherosclerosis Risk-Factors in Male Youngsters)
    Michael Knoflach
    Institute for Biomedical Ageing Research, Austrian Academy of Sciences, Innsbruck, Austria
    Circulation 108:1064-9. 2003
    ..Furthermore, new risk concepts of atherosclerosis focusing on inflammation, infections, and immunity have not yet been evaluated in this age group...
  61. ncbi GroEL-GroES-mediated protein folding
    Arthur L Horwich
    Department of Genetics and Howard Hughes Medical Institute, Yale School of Medicine, Boyer Center, 295 Congress Avenue, New Haven, Connecticut 06510, USA
    Chem Rev 106:1917-30. 2006
  62. pmc Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources
    Alan McNabb
    Laboratory Services, British Columbia Centre for Disease Control, 655 West 12th Ave, Vancouver, British Columbia V5Z 4R4, Canada
    J Clin Microbiol 42:3000-11. 2004
    ....
  63. ncbi rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov
    Toidi Adekambi
    Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, 27, Boulevard Jean Moulin, Université de la Méditerranée and Assistance Publique Hôpitaux de Marseille Timone, Fédération de Microbiologie Clinique, Marseille, France
    Int J Syst Evol Microbiol 56:133-43. 2006
    ....
  64. pmc A systematic survey of in vivo obligate chaperonin-dependent substrates
    Kei Fujiwara
    Department of Medical Genome Sciences, University of Tokyo, FSB401, Kashiwa, Chiba, Japan
    EMBO J 29:1552-64. 2010
    ..Notably, approximately 70% of the Class IV substrates appear to be metabolic enzymes, supporting a hypothetical role of GroE in enzyme evolution...
  65. pmc Chaperonin complex with a newly folded protein encapsulated in the folding chamber
    D K Clare
    Department of Crystallography and Institute for Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Nature 457:107-10. 2009
    ....
  66. ncbi Distinct actions of cis and trans ATP within the double ring of the chaperonin GroEL
    H S Rye
    Howard Hughes Medical Institute, Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut 06510, USA
    Nature 388:792-8. 1997
    ..These observations offer an explanation of why GroEL functions as a double-ring complex...
  67. ncbi Stressed apoptotic tumor cells stimulate dendritic cells and induce specific cytotoxic T cells
    Hanping Feng
    Department of Pediatrics, Steele Memorial Children s Research Center, University of Arizona, Tucson 85724, USA
    Blood 100:4108-15. 2002
    ....
  68. ncbi Hsp60 regulation of tumor cell apoptosis
    Jagadish C Ghosh
    Department of Cancer Biology and the Cancer Center, University of Massachusetts Medical School, Worcester, Massachustetts 01605, USA
    J Biol Chem 283:5188-94. 2008
    ..Hsp60 inhibitors may function as attractive anticancer agents by differentially inducing apoptosis in tumor cells...
  69. ncbi Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4
    Rick de Graaf
    Department of Radiology, Cardiovascular Research Institute Maastricht, University Hospital Maastricht, P Debyelaan 25, 6202 AZ, Maastricht, The Netherlands
    Microbes Infect 8:1859-65. 2006
    ..Moreover, our data surpass the infection- and autoimmunity-based hypotheses of cardiovascular disease and suggest an additional HSP60-related autocrine process...
  70. pmc Pyrosequencing of the chaperonin-60 universal target as a tool for determining microbial community composition
    John Schellenberg
    Department of Medical Microbiology, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba R3E 0W3, Canada
    Appl Environ Microbiol 75:2889-98. 2009
    ..These observations demonstrate that pyrosequencing of cpn60 UT amplicons provides a robust, reliable method for deep sequencing of microbial communities...
  71. pmc Equine and canine Anaplasma phagocytophilum strains isolated on the island of Sardinia (Italy) are phylogenetically related to pathogenic strains from the United States
    Alberto Alberti
    Istituto di Patologia Speciale e Clinica Medica Veterinaria, Universita degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy
    Appl Environ Microbiol 71:6418-22. 2005
    ..Phylogenetic analysis revealed that Sardinian strains are genetically distinct from the two lineages previously described in Europe and are closely related to strains isolated in different areas of the United States...
  72. ncbi miR-1/miR-206 regulate Hsp60 expression contributing to glucose-mediated apoptosis in cardiomyocytes
    Zhi xin Shan
    Research Center of Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China
    FEBS Lett 584:3592-600. 2010
    ..These results revealed that miR-1 and miR-206 regulate Hsp60 expression, contributing to high glucose-mediated apoptosis in cardiomyocytes...
  73. ncbi Detection of HSP60 on the membrane surface of stressed human endothelial cells by atomic force and confocal microscopy
    Gerald Pfister
    Institute for Biophysics, University of Linz, A 4040 Linz, Austria
    J Cell Sci 118:1587-94. 2005
    ..The relevance of our findings with respect to the role of HSP60 in atherogenesis is discussed...
  74. pmc A signal peptide derived from hsp60 binds HLA-E and interferes with CD94/NKG2A recognition
    Jakob Michaelsson
    Microbiology and Tumor Biology Center, Karolinska Institutet, 171 77 Stockholm, Sweden
    J Exp Med 196:1403-14. 2002
    ..Such stress induced peptide interference would gradually uncouple CD94/NKG2A inhibitory recognition and provide a mechanism for NK cells to detect stressed cells in a peptide-dependent manner...
  75. pmc Lactobacillus strain diversity based on partial hsp60 gene sequences and design of PCR-restriction fragment length polymorphism assays for species identification and differentiation
    Giuseppe Blaiotta
    Department of Food Science, School of Agriculture, The University of Naples Federico II, Via Universita 100, 80055 Portici, Italy
    Appl Environ Microbiol 74:208-15. 2008
    ..casei and L. rhamnosus or L. plantarum and L. pentosus) isolated from cheese and dry-fermented sausages...
  76. pmc A function for the mitochondrial chaperonin Hsp60 in the structure and transmission of mitochondrial DNA nucleoids in Saccharomyces cerevisiae
    Brett A Kaufman
    Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    J Cell Biol 163:457-61. 2003
    ..Our data suggest that the nucleoid dynamics underlying mtDNA transmission are regulated by the interaction between Hsp60 and mtDNA ori sequences...
  77. ncbi Mycobacterium tuberculosis heat shock proteins use diverse Toll-like receptor pathways to activate pro-inflammatory signals
    Yonca Bulut
    Division of Pediatric Critical Care, Cedars Sinai Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, California 90048, USA
    J Biol Chem 280:20961-7. 2005
    ....
  78. pmc cpnDB: a chaperonin sequence database
    Janet E Hill
    National Research Council Plant Biotechnology Institute, Saskatoon, Saskatchewan S7N 0W9, Canada
    Genome Res 14:1669-75. 2004
    ..We built cpnDB from open source tools and it is available at http://cpndb.cbr.nrc.ca...
  79. ncbi Fibrillogenic propensity of the GroEL apical domain: a Janus-faced minichaperone
    Jin Chen
    Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, Myodaiji, Okazaki, Japan
    FEBS Lett 586:1120-7. 2012
    ..Thus, the analysis of the behaviour of the apical domain reveals how aggregation and chaperone-mediated anti-aggregation processes can be closely related...
  80. pmc Role of the gamma-phosphate of ATP in triggering protein folding by GroEL-GroES: function, structure and energetics
    Charu Chaudhry
    Department of Molecular Biophysics and Biochemistry and Howard Hughes Medical Institute, Yale University, New Haven, CT, USA
    EMBO J 22:4877-87. 2003
    ..We discuss the likely basis of the ability of gamma-phosphate binding to convert preformed GroEL-GroES-ADP-polypeptide complexes into the folding-active state...
  81. pmc Coarse-grained normal mode analysis in structural biology
    Ivet Bahar
    Department of Computational Biology, University of Pittsburgh, W1043 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Curr Opin Struct Biol 15:586-92. 2005
    ..Such applications have provided great insight into the underlying principles linking protein structures to their dynamics and their dynamics to their functions...
  82. pmc Potato leafroll virus binds to the equatorial domain of the aphid endosymbiotic GroEL homolog
    S A Hogenhout
    Department of Virology, DLO Research Institute for Plant Protection, Wageningen, The Netherlands
    J Virol 72:358-65. 1998
    ..Both the N- and C-terminal regions of the equatorial domain were implicated in virus binding...
  83. pmc A conservative test of genetic drift in the endosymbiotic bacterium Buchnera: slightly deleterious mutations in the chaperonin groEL
    Joshua T Herbeck
    Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Genetics 165:1651-60. 2003
    ..Rather, they further support the hypothesis that drift is an important force driving accelerated protein evolution in this obligate mutualist...
  84. ncbi The effect of nucleotides and mitochondrial chaperonin 10 on the structure and chaperone activity of mitochondrial chaperonin 60
    G Levy-Rimler
    Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel
    Eur J Biochem 268:3465-72. 2001
    ..We propose that the cis (mt-cpn60)14.nucleotide.(mt-cpn10)7 complex is not a stable species and does not bind ADP effectively at its trans binding site...
  85. pmc Heat shock protein 60 is the major antigen which stimulates delayed-type hypersensitivity reaction in the macaque model of Chlamydia trachomatis salpingitis
    Anne B Lichtenwalner
    Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98195 6460, USA
    Infect Immun 72:1159-61. 2004
    ....
  86. ncbi Heat-shock protein 60 translocates to the surface of apoptotic cells and differentiated megakaryocytes and stimulates phagocytosis
    Yaw Chong Goh
    Department of Surgery, Singapore General Hospital, Singapore, Singapore
    Cell Mol Life Sci 68:1581-92. 2011
    ..Our results suggests that externalized Hsp60 in apoptotic cells and senescent platelets influences events subsequent to apoptosis, such as the clearance of apoptotic cells by phagocytes...
  87. ncbi Global unfolding of a substrate protein by the Hsp100 chaperone ClpA
    E U Weber-Ban
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Nature 401:90-3. 1999
    ....
  88. ncbi Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
    Erica Chimara
    Setor de Micobacterias, Secao de Bacteriologia, Instituto Adolfo Lutz, Sao Paulo, Av Dr Arnaldo 355, 01246 902, SP, Brazil
    Mem Inst Oswaldo Cruz 99:739-43. 2004
    ..Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of Sao Paulo was almost exclusively subtype I regardless of HIV status...
  89. ncbi Stressed apoptotic tumor cells express heat shock proteins and elicit tumor-specific immunity
    H Feng
    Department of Pediatrics, Steele Memorial Children's Research Center, University of Arizona, 1501 N. Campbell Ave, Tucson, AZ 85724, USA
    Blood 97:3505-12. 2001
    ..Blood. 2001;97:3505-3512)..
  90. ncbi Trapping of Tomato yellow leaf curl virus (TYLCV) and other plant viruses with a GroEL homologue from the whitefly Bemisia tabaci
    F Akad
    The Minerva Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel
    Arch Virol 149:1481-97. 2004
    ..Predictions pertaining to viruses that do, or do not bind to GroEL, and applications in plant virus diagnosis, are presented...
  91. ncbi Expression and function of a groEL paralog in the thermophilic cyanobacterium Thermosynechococcus elongatus under heat and cold stress
    Shinichiro Sato
    Department of Biochemistry and Molecular Biology, Saitama University, Saitama 338 8570, Japan
    FEBS Lett 582:3389-95. 2008
    ..The groEL2 gene was dispensable under normal growth conditions at 50 degrees C as a groEL2 disruptant was viable. This groEL2 mutant was highly sensitive to both high and low temperatures...
  92. ncbi Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex
    K Ohashi
    German Diabetes Research Institute, Heinrich Heine University, Dusseldorf, Germany
    J Immunol 164:558-61. 2000
    ..We conclude that Tlr4 mediates hsp60 signaling. This is the first report of a putative endogenous ligand of the Tlr4 complex...
  93. ncbi Immunization with a combination of ApoB and HSP60 epitopes significantly reduces early atherosclerotic lesion in Apobtm2SgyLdlrtm1Her/J mice
    Xinjie Lu
    Thrombosis Research Institute, London, United Kingdom
    Atherosclerosis 212:472-80. 2010
    ....
  94. ncbi Predicting relatedness of bacterial genomes using the chaperonin-60 universal target (cpn60 UT): application to Thermoanaerobacter species
    Tobin J Verbeke
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
    Syst Appl Microbiol 34:171-9. 2011
    ....
  95. ncbi Chlamydia heat shock protein 60 induces trophoblast apoptosis through TLR4
    Ozlem Equils
    Department of Pediatrics, Geffen School of Medicine, University of California Los Angeles, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA
    J Immunol 177:1257-63. 2006
    ..These data suggest that TLR4 stimulation induces apoptosis in placenta, and this could provide a novel mechanism of pathogenesis for poor fertility and pregnancy outcome in women with persistent chlamydia infection...
  96. ncbi Dissecting a bimolecular process of MgATP²- binding to the chaperonin GroEL
    Jin Chen
    Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki 444 8787, Japan
    J Mol Biol 410:343-56. 2011
    ..The temperature dependence of the kinetics indicated that MgATP(2)(-) binding to GroEL was activation-controlled with an activation enthalpy as large as 14-16 kcal mol(-1)...
  97. pmc Stimulating the substrate folding activity of a single ring GroEL variant by modulating the cochaperonin GroES
    Melissa Illingworth
    Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana 47405, USA
    J Biol Chem 286:30401-8. 2011
    ..Active GroEL(SR)-GroES(7) systems may resemble mammalian mitochondrial chaperonin systems...
  98. ncbi Characterisation of mutations in GroES that allow GroEL to function as a single ring
    Han Liu
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    FEBS Lett 583:2365-71. 2009
    ..Nearly half of the mutants showed partial function with SR1. Two mutants were confirmed to have reduced affinity for GroEL. Intriguingly, some GroES mutants were able to function with active single ring mutants of GroEL...
  99. pmc The MitCHAP-60 disease is due to entropic destabilization of the human mitochondrial Hsp60 oligomer
    Avital Parnas
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, 69778 Tel Aviv, Israel
    J Biol Chem 284:28198-203. 2009
    ..These results suggest that the D3G mutation leads to entropic destabilization of the mHsp60 oligomer, which severely impairs its chaperone function, thereby causing the disease...
  100. ncbi A molecular enrichment strategy based on cpn60 for detection of epsilon-proteobacteria in the dog fecal microbiome
    Bonnie Chaban
    Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada S7N 5B4
    Microb Ecol 63:348-57. 2012
    ....
  101. ncbi Antibodies against recombinant heat shock proteins of 60 kDa from enterobacteria in the sera and synovial fluid of HLA-B27 positive ankylosing spondylitis patients
    M L Domínguez-López
    Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico
    Clin Exp Rheumatol 27:626-32. 2009
    ..To study the association of HLA-B27 with IgG antibodies to different enterobacterial HSP60s in patients with ankylosing spondylitis (AS)...

Research Grants65

  1. Hsp60 regulation of apoptosis in prostate cancer
    Dhyan Chandra; Fiscal Year: 2013
    ..g., caspase-9, and -8). ..
  2. Combination Antibotics as a Treatment for Chlamydia-Induced Reactive Arthritis
    John Carter; Fiscal Year: 2007
    ..Chronic Chlamydia infections have also been linked to trachoma, tubal infertility, coronary artery disease, asthma, certain types of cervical cancer, and possibly other diseases. [unreadable] [unreadable] [unreadable]..
  3. Gamma Delta T Cells in Anti-Bacterial Host Defense
    Thomas Moore; Fiscal Year: 2005
    ..pneumoniae bacteremia. These studies will provide insights for the development of therapeutic modalities aimed at augmenting host responses, resulting in enhanced resolution of bacterial infections. ..
  4. TrialNet: University of Florida Clinical Center and Network
    Desmond Schatz; Fiscal Year: 2007
    ..It is thus a promising agent to consider for the prevention of Type 1 diabetes. ..
  5. EFFECTIVENESS OF OUTPATIENT TREATMENT OF PID
    Roberta Ness; Fiscal Year: 2003
    ..We gain great efficiency in the use of the extensive resources and excellent follow-up success within the PEACH cohort. ..
  6. PERIVENTRICULAR LEUKOMALACIA IN THE PREMATURE INFANT
    Joseph Volpe; Fiscal Year: 2009
    ..The progress of the overall Program in the first cycle and the proposed work building on that progress should lead to insights that allow formulation of preventative interventions suitable for clinical trial. ..
  7. Mechanisms of ozone-induced pulmonary toxicity
    Anna Vetrano; Fiscal Year: 2004
    ..abstract_text> ..
  8. Reverse Line Blot Daignostic Test for Agents of Sepsis
    Andrew Levin; Fiscal Year: 2007
    ..with blood culture, and subject to PCR amplification using universal degenerate primers for a region of the chaperonin 60 gene (Cpn60 or HSP60) widely conserved among prokaryotic species...
  9. HSP60 ABNORMALITIES IN SCHIZOPHRENIA
    Saud Sadiq; Fiscal Year: 2001
    ..Molecular studies will involve genomic and cDNA cloning of hsp60 from peripheral and brain tissue. Epitope mapping will be done by ELISA using overlapping peptides spanning the entire normal human hsp60 sequence. ..
  10. Chaperonin-mediated protein folding
    Arthur Horwich; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  11. MECHANISM OF ACTION OF THE HSP100 CHAPERONE CLPA
    Arthur Horwich; Fiscal Year: 2006
    ....
  12. PROTECTIVE ANTIGENS FROM HISTOPLASMA CAPSULATUM
    GEORGE DEEPE; Fiscal Year: 2007
    ..abstract_text> ..
  13. Defending Against Systemic Mycoses
    GEORGE DEEPE; Fiscal Year: 2008
    ..The collaborative and synergistic effort manifested by this group of investigators will provide new and exciting information regarding prevention and/or treatment of these problematic fungi. ..
  14. Effect of donor tissue gp96 on allograft immunity
    ALAN POCKLEY; Fiscal Year: 2003
    ..In particular, gp96 would present the alloantigenic fingerprint of the donor to the recipient immune system, and the requirement for tissue cross-matching may be rendered unnecessary. ..
  15. Modulation of Autoimmunity by Green Tea Polyphenols
    KAMAL MOUDGIL; Fiscal Year: 2006
    ..The results of this study would help in establishing a reliable scientific basis for the use of PGT in autoimmune arthritis, and in developing better therapeutic approaches for RA. ..
  16. Pathogenesis of Persistent Chlamydial STDs
    Deborah Dean; Fiscal Year: 2007
    ..1 Description, ..
  17. SELF-DETERMINANTS & INDIRECTION OF ANTI-SELF IMMUNITY
    KAMAL MOUDGIL; Fiscal Year: 2001
    ..They believe that such predetermined, experimentally programmed control of the display hierarchy of cryptic self determinants would pave the way for novel immunotherapeutic strategies for prevention and treatment of cancer. ..
  18. REGULATION OF AUTOIMMUNITY THROUGH EPITOPE SPREADING
    KAMAL MOUDGIL; Fiscal Year: 2006
    ..This in turn would contribute to developing novel therapeutic approaches for RA and other autoimmune diseases. [unreadable] [unreadable]..
  19. PROFILING SYNOVIAL VASCULATURE IN ARTHRITIS-PRONE RATS
    KAMAL MOUDGIL; Fiscal Year: 2005
    ..abstract_text> ..
  20. Multiscale Dynamics of Cell Cycle Control and Apoptosis
    Ivet Bahar; Fiscal Year: 2005
    ....
  21. Computational Prediction of Biomolecular Dynamics
    Ivet Bahar; Fiscal Year: 2007
    ..This novel computational methodology will fill a unique niche due to its applicability to large structures and assemblies, its speed and accessibility to the scientific community. ..
  22. PATHOGENESIS OF CHLAMYDIAL OCULAR INFECTIONS
    Deborah Dean; Fiscal Year: 2004
    ..A greater understanding of the pathogenesis of trachoma will aid in developing public health interventions and a vaccine that can be implemented in trachoma endemic areas throughout the world. ..
  23. C. trachomatis genomics, strain typing, and evolution
    Deborah Dean; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  24. 2008 Spirochetes Gordon Research Conference
    David Haake; Fiscal Year: 2008
    ..Funding from the National Institutes of Health is requested to partially offset the travel and registration expenses of the participating graduate students, fellows, and junior faculty members. ..
  25. International Symposium for Hereditary Spastic Paraplegia
    John Fink; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  26. Hierarchical Methods for Large BioMolecular Complexes
    Chandrajit L Bajaj; Fiscal Year: 2010
    ..AIM 4: To implement, test, package and freely distribute our structural feature determination and fast search/fitting techniques. ..
  27. Molecular Simulations of Folding & Association in Physiological Environments
    ADRIAN HAMILTON ELCOCK; Fiscal Year: 2010
    ..biological and quantitative proteomics data will be utilized to build preliminary 3D models of four different environments in the model prokaryote Escherichia coli: the cytoplasm, periplasm, and inner and outer-membranes ..
  28. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  29. INTERMEDIATES IN PROTEIN FOLDING
    Carl Frieden; Fiscal Year: 2008
    ..Both these projects employ novel real-time and equilibrium NMR methodology in addition to standard. ..
  30. Hereditary Spastic Paraplegia due to SPG3A/atlastin mutation
    John Fink; Fiscal Year: 2009
    ....
  31. CARDIAC HYPERTROPHY AND SERCA2 GENE EXPRESSION
    Wolfgang Dillmann; Fiscal Year: 2008
    ..In addition, we will determine if HF induces changes in Mito Ca handling is correlated with diminished high-energy phosphate production and can be reverted towards normal. [unreadable] [unreadable] [unreadable] [unreadable]..
  32. Gene Repression by HSF1 during hyperthermia and stresses
    Stuart Calderwood; Fiscal Year: 2006
    ..Dominant negative inhibitors of HSF1 repression have been identified in this study and they will be used in transfected cells to study the cellular consequences of gene repression by HSF 1. ..
  33. GENE EXPRESSION BY HUMAN GRANULOCYTIC EHRLICHIOSIS AG
    Yasuko Rikihisa; Fiscal Year: 2004
    ..These efforts are expected to reveal an important role for the p44-multigene family in strategies of adaptive surface variation and avoidance of host immune responses. ..
  34. CD8+ T Cells Control Autoimmunity In EAE
    Hong Jiang; Fiscal Year: 2007
    ..Thus, the studies will provide theoretical basis for clinical application of this regulatory pathway in prevention and treatment of multiple sclerosis in man. [unreadable] [unreadable] [unreadable]..
  35. A New Approach to Rapid Protein-Protein Docking
    Chandrajit Bajaj; Fiscal Year: 2008
    ..abstract_text> ..
  36. ORGANIZATION, EXPRESSION, AND INHERITANCE OF YEAST MTDNA
    RONALD BUTOW; Fiscal Year: 2007
    ..These studies will be coupled with the analysis of cis-acting elements that distinguish active from inactive ori elements. Altogether, these studies have direct implications for understanding mitochondrial diseases in humans. ..
  37. THYROID ACTION IN THE HEART
    Wolfgang Dillmann; Fiscal Year: 2006
    ..In addition the mechanisms underlying hyperthyroidism induced atrial fibrillation will be determined. ..
  38. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  39. Acquisition of an electrospray TOF mass spectrometer
    IGOR KALTASHOV; Fiscal Year: 2004
    ..The operation of the instrument will be overseen by a Mass Spectrometry Advisory committee comprised of faculty members representing three academic departments and one interdisciplinary research center at UMass. ..
  40. Toll-Like Receptors in Bacterial Antigen Induced HIV-LTR
    Ozlem Equils; Fiscal Year: 2004
    ..abstract_text> ..
  41. MOLECULAR BASIS OF PERSISTENT EHRLICHIA INFECTION
    XUE JIE YU; Fiscal Year: 2004
    ..chaffeensis. This will provide valuable information regarding the potential use of these antigens as subunit vaccines. ..
  42. PHOSPHORYLATION OF HSP 72 AND CARDIAC PROTECTION
    ANNE KNOWLTON; Fiscal Year: 2001
    ..These studies will further the ultimate goal of understanding the regulation and function of this endogenous protective protein so that expression can be manipulated for the benefit of cardiac patients. ..
  43. NATURAL HISTORY OF ANAL NEOPLASIA IN HIV INFECTED MEN
    Joel Palefsky; Fiscal Year: 2002
    ....
  44. ROLE OF HSF2 IN THE STRESS RESPONSE TO HYPERTHERMIA
    Stuart Calderwood; Fiscal Year: 2002
    ..abstract_text> ..
  45. HIV/SIV ENVELOPE GLYCOPROTEIN EFFECTS ON ENDOTHELIA
    Changyi Chen; Fiscal Year: 2002
    ....
  46. Bartonella Inhibitory Factor for Endothelial Cell Growth
    MICHAEL MINNICK; Fiscal Year: 2002
    ..abstract_text> ..
  47. HIV PROTEASE INHIBITORS EFFECT--NENDOTHELIAL DYSFUNCTION
    Changyi Chen; Fiscal Year: 2004
    ..Together, the integrated basic science, non-human primate and human investigations offer a multi disciplinary approach to the understanding and prevention of protease inhibitor-associated vascular complications. ..
  48. Gene Expression Levels Across Diverse Genomes
    Samuel Karlin; Fiscal Year: 2006
    ..A third major objective of our research will be to extend our codon usage methods for predicting gene expression levels to eukaryotic genomes, including yeast, D. melanogaster, C. elegans, and human. [unreadable] [unreadable]..
  49. Clinical Epidemiology of Mycoplasma genitalium
    Patricia Totten; Fiscal Year: 2006
    ..Studies of its association with salpingitis and pregnancy morbidity are essential. Future studies should also address whether, similar to gonorrhea and chlamydial infection, it facilitates transmission of HIV infection. ..
  50. AGING, ESTROGEN, HSPS AND MYOCARDIAL ISCHEMIA
    ANNE KNOWLTON; Fiscal Year: 2005
    ..The long-term goal of this research initiative is to understand gender differences in the HSPs and changes in the HSPs with aging in the heart. ..
  51. Conference on Transmissible Spongiform Encephalopathies
    Adriano Aguzzi; Fiscal Year: 2005
    ....
  52. GENETIC AND MOLECULAR STUDIES OF GROUP II INTRONS
    RONALD BUTOW; Fiscal Year: 2004
    ..Finally, the RNA binding and splicing functions of the naturase domain of the intron-encoded protein will be studied. ..
  53. Molecular Surgeon Symposium on Genetics and Genomics of Pancreatic Cancer
    Changyi Chen; Fiscal Year: 2008
    ..Furthermore, invited speakers are encouraged to submit manuscripts related to their presentations to "World Journal of Surgery" for consideration of publications. [unreadable] [unreadable] [unreadable]..
  54. ANGIOTENSIN II AND REGULATION OF MCP-1 GENE EXPRESSION
    Changyi Chen; Fiscal Year: 2002
    ....
  55. Biophysical studies of a Sulfolobus icosahedral virus
    Reza Khayat; Fiscal Year: 2007
    ..Detailed characterization of Archaea viruses may also expand our knowledge of viral evolution. ..
  56. CD8+ T CELL RECOGNITION OF TH1 AND TH2
    Hong Jiang; Fiscal Year: 2003
    ..We will determine if this difference uniquely applies to the recognition of TCR peptide presented by Qa-1 or if CD8+ T cells specific for other peptides presented by MHC class I molecules also distinguish between TH1 and TH2 targets. ..
  57. Effects of HAART drugs on endothelial dysfunction of pu*
    Changyi Chen; Fiscal Year: 2009
    ..abstract_text> ..
  58. Molecular Surgeon Symposium on Vascular Injury, Repair
    Changyi Chen; Fiscal Year: 2005
    ..A pre-approved letter for acceptance of submission of this application from NIH/NHLBI is included. ..
  59. Tissue engineering of small diameter vascular graft
    Changyi Chen; Fiscal Year: 2006
    ..Success of this proposal will directly indicate the clinical applications of tissue engineered small diameter vascular grafts. ..
  60. Effects of HIV Protease Inhibitors of Endothelial Barrie
    Changyi Chen; Fiscal Year: 2005
    ..Our findings may also suggest new therapeutic strategies, which may eventually prove effective for the prevention and treatment of these complications. ..