chaperonins

Summary

Summary: A class of sequence-related MOLECULAR CHAPERONES found in bacteria, mitochondria, and plastids. Chaperonins are abundant constitutive proteins that increase in amount after stresses such as heat shock, bacterial infection of macrophages, and an increase in the cellular content of unfolded proteins. Bacterial chaperonins are major immunogens in human bacterial infections because of their accumulation during the stress of infection. Two members of this class of chaperones are CHAPERONIN 10 and CHAPERONIN 60.

Top Publications

  1. pmc BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly
    Seongjin Seo
    Department of Pediatrics, and Howard Hughes Medical Institute, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 107:1488-93. 2010
  2. ncbi Knot formation in newly translated proteins is spontaneous and accelerated by chaperonins
    Anna L Mallam
    Department of Chemistry, The University of Cambridge, Cambridge, UK
    Nat Chem Biol 8:147-53. 2012
  3. pmc Two cofactors and cytoplasmic chaperonin are required for the folding of alpha- and beta-tubulin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mol Cell Biol 13:2478-85. 1993
  4. ncbi A cytoplasmic chaperonin that catalyzes beta-actin folding
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Cell 69:1043-50. 1992
  5. ncbi Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4
    L Stepanova
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 10:1491-502. 1996
  6. ncbi Multiple chaperonins in bacteria--why so many?
    Peter A Lund
    School of Biosciences, University of Birmingham, Birmingham, UK
    FEMS Microbiol Rev 33:785-800. 2009
  7. pmc Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California
    J S Chae
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA
    J Clin Microbiol 38:1364-9. 2000
  8. ncbi cDNA sequence and overexpression of chloroplast chaperonin 21 from Arabidopsis thaliana
    T Hirohashi
    Institute for Protein Research, Osaka University, Japan
    Biochim Biophys Acta 1429:512-5. 1999
  9. ncbi On the oligomeric state of chloroplast chaperonin 10 and chaperonin 20
    Rajach Sharkia
    George S Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel
    Biochim Biophys Acta 1651:76-84. 2003
  10. ncbi Chloroplasts have a novel Cpn10 in addition to Cpn20 as co-chaperonins in Arabidopsis thaliana
    Y Koumoto
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    J Biol Chem 276:29688-94. 2001

Research Grants

Detail Information

Publications270 found, 100 shown here

  1. pmc BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly
    Seongjin Seo
    Department of Pediatrics, and Howard Hughes Medical Institute, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 107:1488-93. 2010
    ..a novel complex composed of three chaperonin-like BBS proteins (BBS6, BBS10, and BBS12) and CCT/TRiC family chaperonins mediates BBSome assembly, which transports vesicles to the cilia...
  2. ncbi Knot formation in newly translated proteins is spontaneous and accelerated by chaperonins
    Anna L Mallam
    Department of Chemistry, The University of Cambridge, Cambridge, UK
    Nat Chem Biol 8:147-53. 2012
    ..These results explain how knotted protein structures have withstood evolutionary pressures despite their topological complexity...
  3. pmc Two cofactors and cytoplasmic chaperonin are required for the folding of alpha- and beta-tubulin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mol Cell Biol 13:2478-85. 1993
    Though the chaperonins that mediate folding in prokaryotes, mitochondria, and chloroplasts have been relatively well characterized, the folding of proteins in the eukaryotic cytosol is much less well understood...
  4. ncbi A cytoplasmic chaperonin that catalyzes beta-actin folding
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Cell 69:1043-50. 1992
    ..The eukaryotic cytoplasm thus contains a chaperonin that is functionally analagous to its prokaryotic, mitochondrial, and chloroplastic counterparts...
  5. ncbi Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4
    L Stepanova
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 10:1491-502. 1996
    ..This study suggests a general role for p50Cdc37 in signaling pathways dependent on intrinsically unstable protein kinases and reveals a previously unrecognized chaperone-dependent step in the production of Cdk4/cyclin D complexes...
  6. ncbi Multiple chaperonins in bacteria--why so many?
    Peter A Lund
    School of Biosciences, University of Birmingham, Birmingham, UK
    FEMS Microbiol Rev 33:785-800. 2009
    A significant proportion of bacteria express two or more chaperonin genes. Chaperonins are a group of molecular chaperones, defined by sequence similarity, required for the folding of some cellular proteins...
  7. pmc Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California
    J S Chae
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA
    J Clin Microbiol 38:1364-9. 2000
    ..equi isolates from northern California. These results suggest that E. equi and the HGE agent found in California are similar or identical but may differ from the isolates of equine and human origin found in the eastern United States...
  8. ncbi cDNA sequence and overexpression of chloroplast chaperonin 21 from Arabidopsis thaliana
    T Hirohashi
    Institute for Protein Research, Osaka University, Japan
    Biochim Biophys Acta 1429:512-5. 1999
    ..Gel-filtration and chemical cross-linking analyses showed that the recombinant mature AtCpn21 protein forms a stable homo-oligomer composed of three or four polypeptides...
  9. ncbi On the oligomeric state of chloroplast chaperonin 10 and chaperonin 20
    Rajach Sharkia
    George S Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel
    Biochim Biophys Acta 1651:76-84. 2003
    Type I chaperonins are fundamental protein folding machineries that function in eubacteria, mitochondria and chloroplasts...
  10. ncbi Chloroplasts have a novel Cpn10 in addition to Cpn20 as co-chaperonins in Arabidopsis thaliana
    Y Koumoto
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    J Biol Chem 276:29688-94. 2001
    ..The phylogenic tree that was generated with amino acid sequences of other co-chaperonins indicates that chl-Cpn10 is highly divergent from the others...
  11. pmc Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene
    F Brunello
    Dipartimento di Patologia, Sezione di Microbiologia, , Italy
    J Clin Microbiol 39:2799-806. 2001
    ....
  12. pmc In vivo function of Hsp90 is dependent on ATP binding and ATP hydrolysis
    W M Obermann
    Department of Cellular Biochemistry, Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Cell Biol 143:901-10. 1998
    ..Our results establish Hsp90 as an ATP-dependent chaperone...
  13. ncbi A novel Hsp90 inhibitor to disrupt Hsp90/Cdc37 complex against pancreatic cancer cells
    Tao Zhang
    Division of Pharmaceutics, College of Pharmacy, The Ohio State University, 500 West 12th Avenue, Columbus, OH 43210, USA
    Mol Cancer Ther 7:162-70. 2008
    ..The data suggest that celastrol is a novel Hsp90 inhibitor to disrupt Hsp90-Cdc37 interaction against pancreatic cancer cells...
  14. ncbi Substantial CCT activity is required for cell cycle progression and cytoskeletal organization in mammalian cells
    Julie Grantham
    Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Exp Cell Res 312:2309-24. 2006
    ..There are no large-scale effects on cytoplasmic protein synthesis or a general heat shock response during periods of low CCT activity...
  15. ncbi Homologous plant and bacterial proteins chaperone oligomeric protein assembly
    S M Hemmingsen
    Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada
    Nature 333:330-4. 1988
    ..encoding the chloroplast protein has revealed that these proteins are evolutionary homologues which we term 'chaperonins'...
  16. pmc Arabidopsis thaliana type I and II chaperonins
    J E Hill
    National Research Council, Plant Biotechnology Institute, Saskatoon, SK, Canada
    Cell Stress Chaperones 6:190-200. 2001
    ....
  17. pmc The Hsp70 and TRiC/CCT chaperone systems cooperate in vivo to assemble the von Hippel-Lindau tumor suppressor complex
    Mark W Melville
    Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
    Mol Cell Biol 23:3141-51. 2003
    ..We conclude that, in vivo, folding of some polypeptides requires the cooperation of Hsp70 and TRiC and that Hsp70 acts to promote substrate binding to TRiC...
  18. ncbi Hsp90 and Cdc37 -- a chaperone cancer conspiracy
    Laurence H Pearl
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London, SW3 6JB, UK
    Curr Opin Genet Dev 15:55-61. 2005
    ..The central role of the Hsp90-Cdc37 chaperone complex makes it an important target for future anti-cancer drug development...
  19. ncbi The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol
    H Kubota
    Cancer Research Campaign Centre for Cell and Molecular Biology, Institute of Cancer Research, Chester Beatty Laboratories, London, England
    Eur J Biochem 230:3-16. 1995
    ..This complex behaviour of CCT may have evolved to cope with the folding and assembly of certain highly evolved proteins in eukaryotic cells...
  20. ncbi Mycobacterium tuberculosis heat shock proteins use diverse Toll-like receptor pathways to activate pro-inflammatory signals
    Yonca Bulut
    Division of Pediatric Critical Care, Cedars Sinai Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, California 90048, USA
    J Biol Chem 280:20961-7. 2005
    ....
  21. ncbi Mega-Dalton biomolecular motion captured from electron microscopy reconstructions
    Pablo Chacon
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 326:485-92. 2003
    ....
  22. pmc Identification of the TRiC/CCT substrate binding sites uncovers the function of subunit diversity in eukaryotic chaperonins
    Christoph Spiess
    Department of Biological Sciences and BioX Program, Stanford University, Stanford, CA 94305, USA
    Mol Cell 24:25-37. 2006
    ..localizes to a helical region in the apical domains that is structurally equivalent to that of bacterial chaperonins. Transferring the distal portion of helix 11 between TRiC subunits suffices to transfer specificity for a given ..
  23. ncbi The cytosolic chaperonin CCT associates to cytoplasmic microtubular structures during mammalian spermiogenesis and to heterochromatin in germline and somatic cells
    Sylvie Soues
    Laboratoire de Biologie Cellulaire, Centre Universitaire des Saints Peres, Universite Rene Descartes Paris V, 45 Rue des Saints Peres, 75270 Paris Cedex 06, France
    Exp Cell Res 288:363-73. 2003
    ..Our finding that CCT is associated with constitutive heterochromatin and to compacting chromatin raises the possibility that it may be implicated in maintenance and remodelling of heterochromatin...
  24. ncbi Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998
    Erica Chimara
    Setor de Micobacterias, Secao de Bacteriologia, Instituto Adolfo Lutz, Sao Paulo, Av Dr Arnaldo 355, 01246 902, SP, Brazil
    Mem Inst Oswaldo Cruz 99:739-43. 2004
    ..Two isolates were classified as type II and III and five isolates were characterized as other Mycobacterium species. Clinical isolates of M. kansasii in the state of Sao Paulo was almost exclusively subtype I regardless of HIV status...
  25. pmc Withaferin A targets heat shock protein 90 in pancreatic cancer cells
    Yanke Yu
    Department of Pharmaceutical Sciences, College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, MI 48109, USA
    Biochem Pharmacol 79:542-51. 2010
    ....
  26. ncbi Pathways of chaperone-mediated protein folding in the cytosol
    Jason C Young
    Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
    Nat Rev Mol Cell Biol 5:781-91. 2004
    ....
  27. ncbi Molecular chaperones and protein kinase quality control
    Avrom J Caplan
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Trends Cell Biol 17:87-92. 2007
    ..This requirement might relate to conformational changes that take place during the protein kinase activity cycle...
  28. pmc Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT
    K A Won
    Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell Biol 18:7584-9. 1998
    ..Thus, both the acquisition of the native state and turnover of cyclin E involve ATP-dependent processes mediated by large oligomeric assemblies...
  29. pmc The middle domain of Hsp90 acts as a discriminator between different types of client proteins
    Patricija Hawle
    Protein Folding Group, Institute for Genetics, University of Bonn, Römerstr 164, Bonn, Germany
    Mol Cell Biol 26:8385-95. 2006
    ..Thus, Hsp90M seems to discriminate between different substrate types and to adjust the molecular chaperone for proper substrate activation...
  30. ncbi Tumorigenic mutations in VHL disrupt folding in vivo by interfering with chaperonin binding
    Douglas E Feldman
    Department of Biological Sciences and BioX Program, Stanford University, E200A James Clark Center, 318 Campus Drive, Stanford, CA 94305, USA
    Mol Cell 12:1213-24. 2003
    ..Our findings reveal a class of disease-causing mutations that inactivate protein function by disrupting chaperone-mediated folding in vivo...
  31. ncbi Akt forms an intracellular complex with heat shock protein 90 (Hsp90) and Cdc37 and is destabilized by inhibitors of Hsp90 function
    Andrea D Basso
    Program in Pharmacology, Weill Graduate School of Medical Sciences, Cornell University and the Program in Cell Biology and Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 277:39858-66. 2002
    ..Thus, transduction of growth factor signaling via the Akt and Raf pathways requires functional Hsp90 and can be coordinately blocked by its inhibition...
  32. pmc Expression profiles and physiological roles of two types of molecular chaperonins from the hyperthermophilic archaeon Thermococcus kodakarensis
    Shinsuke Fujiwara
    Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo 669 1337, Japan
    Appl Environ Microbiol 74:7306-12. 2008
    Thermococcus kodakarensis possesses two chaperonins, CpkA and CpkB, and their expression is induced by the downshift and upshift, respectively, of the cell cultivation temperature...
  33. ncbi Chloroplast Cpn20 forms a tetrameric structure in Arabidopsis thaliana
    Y Koumoto
    Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
    Plant J 17:467-77. 1999
    ..The analysis on the subunit stoichiometry of the GroEL-Cpn20 complex also revealed that the functional complex is composed of a GroEL tetradecamer and a Cpn20 tetramer...
  34. pmc Two yeast genes with similarity to TCP-1 are required for microtubule and actin function in vivo
    X Chen
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Proc Natl Acad Sci U S A 91:9111-5. 1994
    ..These results indicate that Bin2p and Bin3p are components of a yeast cytoplasmic chaperonin complex that is required for assembly of microtubules and actin in vivo...
  35. pmc Targeting the oncogene and kinome chaperone CDC37
    Phillip J Gray
    Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, Massachusetts 02215, USA
    Nat Rev Cancer 8:491-5. 2008
    ..CDC37 is thus a candidate for broad-spectrum molecular cancer therapy...
  36. pmc Silencing the cochaperone CDC37 destabilizes kinase clients and sensitizes cancer cells to HSP90 inhibitors
    J R Smith
    Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Surrey, UK
    Oncogene 28:157-69. 2009
    ..These results support an essential role for CDC37 in concert with HSP90 in maintaining oncogenic protein kinase clients and endorse the therapeutic potential of targeting CDC37 in cancer...
  37. pmc The effect of chaperonin buffering on protein evolution
    Tom A Williams
    Department of Genetics, University of Dublin, Trinity College, Dublin, Ireland
    Genome Biol Evol 2:609-19. 2010
    ..fate of GroEL clients in the Mycoplasmas, a group of bacteria containing the only known organisms that lack chaperonins. We show that GroEL was lost once in the common ancestor of a monophyletic subgroup of Mycoplasmas, and we ..
  38. ncbi Overexpressed ribosomal proteins suppress defective chaperonins in Saccharomyces cerevisiae
    M Anaul Kabir
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    FEMS Yeast Res 8:1236-44. 2008
    ..We suggest that certain overexpressed ribosomal and other proteins can act as weak chaperones, phenotypically alleviating the partial defects of mutationally altered Cct subunits...
  39. pmc A novel cochaperonin that modulates the ATPase activity of cytoplasmic chaperonin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    J Cell Biol 125:989-96. 1994
    ..Moreover, while GroES functions as a heptameric ring, cofactor A behaves as a dimer. Thus, cofactor A is a novel cochaperonin that is structurally unrelated to GroES...
  40. pmc Mechanism of lid closure in the eukaryotic chaperonin TRiC/CCT
    Christopher R Booth
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, One Baylor Plaza, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Struct Mol Biol 15:746-53. 2008
    All chaperonins mediate ATP-dependent polypeptide folding by confining substrates within a central chamber...
  41. ncbi Cpn20: siamese twins of the chaperonin world
    Celeste Weiss
    Department of Biochemistry, Tel Aviv University, Tel Aviv, Israel
    Plant Mol Biol 69:227-38. 2009
    ..Lastly, we hypothesize as to the oligomeric structure and raison d'être of this unusual co-chaperonin homolog...
  42. ncbi The substrate recognition mechanisms in chaperonins
    Paulino Gomez-Puertas
    Centro de Astrobiología CSIC INTA Ctra Torrejon Ajalvir, Km 4, Torrejon de Ardoz, 28850 Madrid, Spain
    J Mol Recognit 17:85-94. 2004
    b>Chaperonins are a family of proteins devoted to assisting the folding of other proteins. They are large oligomers assembled into ring structures that enclose a cavity in which folding takes place...
  43. ncbi GroE is vital for cell-wall synthesis
    N McLennan
    Nature 392:139. 1998
  44. pmc Molecular evidence of Anaplasma phagocytophilum in Ixodes ricinus ticks and wild animals in Austria
    Helene Polin
    Department of Neurology, Christian Doppler Klinik, Salzburg, Austria
    J Clin Microbiol 42:2285-6. 2004
    ..phagocytophilum...
  45. pmc Aha1 competes with Hop, p50 and p23 for binding to the molecular chaperone Hsp90 and contributes to kinase and hormone receptor activation
    Anja Harst
    Protein Folding Group, Institute for Genetics, University of Bonn, Römerstr 164, D 53117 Bonn, Germany
    Biochem J 387:789-96. 2005
    ....
  46. ncbi Roles of molecular chaperones in protein misfolding diseases
    Jose M Barral
    Department of Cellular Biochemistry, Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Semin Cell Dev Biol 15:17-29. 2004
    ..Increased chaperone expression can suppress the neurotoxicity of these molecules, suggesting possible therapeutic strategies...
  47. pmc CCT chaperonin complex is required for the biogenesis of functional Plk1
    Xiaoqi Liu
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    Mol Cell Biol 25:4993-5010. 2005
    ..Finally, the CCT-depleted cells reenter the cell cycle upon reintroduction of the purified constitutively active form of Plk1, indicating that Plk1 might be a CCT substrate...
  48. pmc Chaperonins
    N A Ranson
    Department of Crystallography, Birkbeck College London, Malet Street, London WC1E 7HX
    Biochem J 333:233-42. 1998
    ..The chaperonins are large, double-ring oligomeric proteins that act as containers for the folding of other protein subunits...
  49. ncbi Regulation of Hsp90 ATPase activity by the co-chaperone Cdc37p/p50cdc37
    Giuliano Siligardi
    Pharmaceutical Optical Spectroscopy Centre, Department of Pharmacy, The Division of Life Sciences, King s College London, United Kingdom
    J Biol Chem 277:20151-9. 2002
    ..However, unlike Sti1/Hop/p60, which can displace geldanamycin upon binding to Hsp90, Cdc37p/p50(cdc37) forms a stable complex with geldanamycin-bound Hsp90 and may be sequestered in geldanamycin-inhibited Hsp90 complexes in vivo...
  50. ncbi Chaperonin TRiC promotes the assembly of polyQ expansion proteins into nontoxic oligomers
    Christian Behrends
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried
    Mol Cell 23:887-97. 2006
    ..We suggest that TRiC cooperates with the Hsp70 system as a key component in the cellular defense against amyloid-like protein misfolding...
  51. pmc Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources
    Alan McNabb
    Laboratory Services, British Columbia Centre for Disease Control, 655 West 12th Ave, Vancouver, British Columbia V5Z 4R4, Canada
    J Clin Microbiol 42:3000-11. 2004
    ....
  52. pmc Chaperonin-mediated folding of actin and tubulin
    S A Lewis
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    J Cell Biol 132:1-4. 1996
  53. ncbi Organelle-specific cochaperonins in apicomplexan parasites
    Shigeharu Sato
    Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Mol Biochem Parasitol 141:133-143. 2005
    ..The apicomplexan Cpn10 is phylogenetically related to other mitochondrial versions but a significant relationship between apicomplexan Cpn20s and other cochaperonins was not established...
  54. ncbi Cytoplasmic chaperonin containing TCP-1: structural and functional characterization
    R Melki
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Biochemistry 36:5817-26. 1997
    ..A model summarizes substrate association with CCT and the role of the nucleotide in regulating the affinity of CCT for target proteins...
  55. ncbi A more precise characterization of chaperonin substrates
    Emanuele Raineri
    CNAG Centro Nacional de Análisis Genómico, Parc Cientific de Barcelona, Baldiri Reixac 4, E 08028 Barcelona, Spain
    Bioinformatics 26:1685-9. 2010
    ..However, a long-standing question in the field is: What makes a protein a chaperone substrate?..
  56. ncbi Aha1 binds to the middle domain of Hsp90, contributes to client protein activation, and stimulates the ATPase activity of the molecular chaperone
    Gregor P Lotz
    Protein Folding Group, Institute for Genetics, University of Bonn, Romerstrasse 164, D 53117 Bonn, Germany
    J Biol Chem 278:17228-35. 2003
    ..Thus, our results identify a novel type of cofactor involved in the regulation of the molecular chaperone Hsp90...
  57. ncbi Recurrent paralogy in the evolution of archaeal chaperonins
    J M Archibald
    Program in Evolutionary Biology Canadian Institute for Advanced Research Department of Biochemistry and Molecular Biology Dalhousie University Halifax, Nova Scotia, B3H 4H7, Canada ca
    Curr Biol 9:1053-6. 1999
    b>Chaperonins are multisubunit double-ring complexes that mediate the folding of nascent proteins [1] [2]. In bacteria, chaperonins are homo-oligomeric and are composed of seven-membered rings...
  58. pmc Do chaperonins boost protein yields by accelerating folding or preventing aggregation?
    A I Jewett
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, USA
    Biophys J 94:2987-93. 2008
    ....
  59. pmc Nucleotide-dependent protein folding in the type II chaperonin from the mesophilic archaeon Methanococcus maripaludis
    Andrew R Kusmierczyk
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Box G J2, Providence, RI 02912, USA
    Biochem J 371:669-73. 2003
    ..In contrast with other known archaeal chaperonins, Mm-cpn is fully functional in all respects under physiological conditions of 37 degrees C...
  60. pmc Novel allelic variants of Mycobacteria isolated in Brazil as determined by PCR-restriction enzyme analysis of hsp65
    A da Silva Rocha
    Biochemistry and Molecular Biology Departmentand, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
    J Clin Microbiol 40:4191-6. 2002
    ..Patterns available on the Internet can be introduced into this database, which will make possible the comparison of genotypes from isolates from different parts of the world...
  61. ncbi Crystal structures of the group II chaperonin from Thermococcus strain KS-1: steric hindrance by the substituted amino acid, and inter-subunit rearrangement between two crystal forms
    Yasuhito Shomura
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Mol Biol 335:1265-78. 2004
    The crystal structures of the group II chaperonins consisting of the alpha subunit with amino acid substitutions of G65C and/or I125T from the hyperthermophilic archaeum Thermococcus strain KS-1 were determined...
  62. pmc Identifying natural substrates for chaperonins using a sequence-based approach
    George Stan
    Laboratory of Computational Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Protein Sci 14:193-201. 2005
    ..Our method also predicts the putative binding regions in the identified SPs. The results of our study show that a variety of SPs, associated with diverse functions, can interact with GroEL...
  63. ncbi Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae
    C Y Turenne
    National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada
    Int J Syst Evol Microbiol 54:1543-51. 2004
    ..nov. is proposed, follows the present trend of a large number of novel Mycobacterium species identified due in great part to sequence-based methods. The type strain is HSC68T (= ATCC BAA-614T = DSM 44648T)...
  64. ncbi Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean
    Sylvia Cardoso Leao
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Botucatu, 862 3 degrees andar, São Paulo 04023 062, Brazil
    J Microbiol Methods 61:193-9. 2005
    ..In others, improvement in critical points is still necessary...
  65. pmc An outbreak of keratitis caused by Mycobacterium immunogenum
    Jorge Luiz Mello Sampaio
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Botucatu, 862 3 degrees andar, 04023 062 Sao Paulo, Brazil
    J Clin Microbiol 44:3201-7. 2006
    ..This is the first report of an outbreak where this species was isolated from infected tissues...
  66. ncbi The unusual mycobacterial chaperonins: evidence for in vivo oligomerization and specialization of function
    Mingqi Fan
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 85:934-44. 2012
    The pathogen Mycobacterium tuberculosis expresses two chaperonins, one (Cpn60.1) dispensable and one (Cpn60.2) essential...
  67. ncbi Domain-mediated dimerization of the Hsp90 cochaperones Harc and Cdc37
    John Roiniotis
    Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
    Biochemistry 44:6662-9. 2005
    ..Thus Harc could potentially contribute to the regulation of the Hsp90-mediated folding of Cdc37-dependent protein kinases into functional conformations via dimerization with Cdc37...
  68. ncbi Mycobacterium hsp65 DNA entrapped into TDM-loaded PLGA microspheres induces protection in mice against Leishmania (Leishmania) major infection
    Eduardo Antonio Ferraz Coelho
    Sector of Clinical Pathology, COLTEC, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, 31 270 901, Belo Horizonte, Minas Gerais, Brazil
    Parasitol Res 98:568-75. 2006
    ..These findings indicate that encapsulated MHSP/TDM is more immunogenic than naked hsp65 DNA, and has great potential to improve vaccine effectiveness against leishmaniasis and tuberculosis...
  69. pmc Mycobacterium avium in the postgenomic era
    Christine Y Turenne
    McGill University Health Centre, A5 156, 1650 Cedar Avenue, Montreal H3G 1A4, Canada
    Clin Microbiol Rev 20:205-29. 2007
    ..avium subsets in isolation, it is expected that attention to the similarities and differences between M. avium organisms will provide greater insight into their fundamental differences, including their propensity to cause disease...
  70. ncbi Review: the Cct eukaryotic chaperonin subunits of Saccharomyces cerevisiae and other yeasts
    V Stoldt
    Heinrich Heine Universitat, Institut fur Mikrobiologie, Dusseldorf, Germany
    Yeast 12:523-9. 1996
    ..Cct8p from C. albicans, the only other completely sequenced Cct protein from a fungal species other than S. cerevisiae, is 72% and 61% similar to the S. cerevisiae and mouse Cct8 proteins, respectively...
  71. ncbi Conformational rearrangements of an archaeal chaperonin upon ATPase cycling
    I Gutsche
    Max Planck Institute for Biochemistry, Martinsried bei Munchen, D 82152, Germany
    Curr Biol 10:405-8. 2000
    b>Chaperonins are double-ring protein assemblies with a central cavity that provides a sequestered environment for in vivo protein folding...
  72. ncbi Chaperonins facilitate KNOTTED1 cell-to-cell trafficking and stem cell function
    Xianfeng Morgan Xu
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 333:1141-4. 2011
    ..Genetic and physical interaction data show a functional relevance for chaperonins in KNOX family-dependent stem cell maintenance...
  73. pmc V600E B-Raf requires the Hsp90 chaperone for stability and is degraded in response to Hsp90 inhibitors
    O M Grbovic
    Program in Molecular Pharmacology and Chemistry and Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:57-62. 2006
    ..Hsp90 inhibition represents a therapeutic strategy for the treatment of melanoma...
  74. ncbi Conserved sequence motifs in bacterial and bacteriophage chaperonins
    E V Koonin
    National Library of Medicine, National Institute of Health, Bethesda, MD 20894
    Trends Biochem Sci 20:14-5. 1995
  75. ncbi Targeting CDC37: an alternative, kinase-directed strategy for disruption of oncogenic chaperoning
    Jennifer R Smith
    Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK
    Cell Cycle 8:362-72. 2009
    ..Here we discuss the therapeutic possibilities of targeting CDC37 for cancer treatment in light of recent significant findings...
  76. ncbi Chaperonins: The hunt for the Group II mechanism
    Maria Giulia Bigotti
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol B58 1TD, UK
    Arch Biochem Biophys 474:331-9. 2008
    b>Chaperonins are multi-subunit complexes that enhance the efficiency of protein-folding reactions by capturing protein substrates in their central cavities...
  77. ncbi Cyclin D1 overexpression is not a specific grouping marker, but may collaborate with CDC37 in myeloma cells
    Yuta Katayama
    Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Japan
    Int J Oncol 25:579-95. 2004
    ..The CDC37 may collaborate with cyclin D1 in progression of MM...
  78. ncbi Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin
    I E Vainberg
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    Cell 93:863-73. 1998
    ..We show that by directing target proteins to chaperonin, prefoldin promotes folding in an environment in which there are many competing pathways for nonnative proteins...
  79. pmc Multiple states of a nucleotide-bound group 2 chaperonin
    Daniel K Clare
    Department of Crystallography, Birkbeck College and Institute of Structural Molecular Biology, University of London, London WC1E 7HX, United Kingdom
    Structure 16:528-34. 2008
    ....
  80. ncbi The CCT/TRiC chaperonin is required for maturation of sphingosine kinase 1
    Julia R Zebol
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    Int J Biochem Cell Biol 41:822-7. 2009
    ..Thus, combined this data suggests that SK1 is a CCT/TRiC substrate, and that this chaperonin facilitates folding of newly translated SK1 into its mature active form...
  81. ncbi Gene duplication and gene conversion shape the evolution of archaeal chaperonins
    John M Archibald
    Program in Evolutionary Biology, Canadian Institute for Advanced Research Department of Biochemistry and Molecular Biology, Halifax, Nova Scotia, B3H 4H7 Canada
    J Mol Biol 316:1041-50. 2002
    b>Chaperonins are multi-subunit double-ring complexes that mediate the folding of nascent or denatured proteins. Gene duplication has been a potent force in the evolution of chaperonins in Archaea...
  82. ncbi p50(cdc37) binds directly to the catalytic domain of Raf as well as to a site on hsp90 that is topologically adjacent to the tetratricopeptide repeat binding site
    A M Silverstein
    Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 273:20090-5. 1998
    ..p50(cdc37) composition that is observed with a variety of protein kinase heterocomplexes immunoadsorbed from cytosols...
  83. ncbi Protein kinase CK2 in health and disease: CK2: the kinase controlling the Hsp90 chaperone machinery
    Y Miyata
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto, 606 8502, Japan
    Cell Mol Life Sci 66:1840-9. 2009
    ..The tumor kinome appears to become addicted to the Hsp90-Cdc37 chaperone system, thus, targeting Hsp90, Cdc37, and CK2 is a promising strategy for cancer treatment...
  84. pmc Archaeal-like chaperonins in bacteria
    Stephen M Techtmann
    Institute of Marine and Environmental Technology, Program in the Biology of Model Systems, 701 East Pratt Street, Baltimore, MD 21202, USA
    Proc Natl Acad Sci U S A 107:20269-74. 2010
    b>Chaperonins (CPN) are ubiquitous oligomeric protein machines that mediate the ATP-dependent folding of polypeptide chains...
  85. ncbi GroEL/GroES-dependent reconstitution of alpha2 beta2 tetramers of humanmitochondrial branched chain alpha-ketoacid decarboxylase. Obligatory interaction of chaperonins with an alpha beta dimeric intermediate
    J L Chuang
    Departments of Biochemistry and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
    J Biol Chem 274:10395-404. 1999
    ..denatured in 8 M urea were competently reconstituted in vitro at 23 degrees C with an absolute requirement for chaperonins GroEL/GroES and Mg-ATP...
  86. ncbi Eukaryotic type II chaperonin CCT interacts with actin through specific subunits
    O Llorca
    Centro Nacional de Biotecnologia, C S I C, Campus Universidad Autonoma de Madrid, Spain
    Nature 402:693-6. 1999
    b>Chaperonins assist the folding of other proteins. Type II chaperonins, such as chaperonin containing TCP-1(CCT), are found in archaea and in the eukaryotic cytosol...
  87. ncbi Co-chaperone regulation of conformational switching in the Hsp90 ATPase cycle
    Giuliano Siligardi
    Pharmaceutical Optical Spectroscopy Centre and Division of Life Sciences, Department of Pharmacy, King s College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NN, United Kingdom
    J Biol Chem 279:51989-98. 2004
    ..These data indicated that Sba1 and Aha1 regulate Hsp90 by influencing the conformational state of the "ATP lid" and consequent N-terminal dimerization, whereas Sti1 does not...
  88. ncbi Pathway leading to correctly folded beta-tubulin
    G Tian
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    Cell 86:287-96. 1996
    ..Sequence analysis identifies yeast homologs of cofactors D (cin1) and E (pac2), characterized by mutations that affect microtubule function...
  89. ncbi Formation of the VHL-elongin BC tumor suppressor complex is mediated by the chaperonin TRiC
    D E Feldman
    Department of Biological Sciences, Stanford University, California 94305, USA
    Mol Cell 4:1051-61. 1999
    ..Our results define a novel role for TRiC in mediating oligomerization and suggest that inactivating mutations can impair polypeptide function by interfering with chaperone-mediated folding...
  90. ncbi Immunotherapy with plasmid DNA encoding mycobacterial hsp65 in association with chemotherapy is a more rapid and efficient form of treatment for tuberculosis in mice
    C L Silva
    REDE TB, Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Gene Ther 12:281-7. 2005
    ....
  91. ncbi Hsp60 expression, new locations, functions and perspectives for cancer diagnosis and therapy
    Francesco Cappello
    Department of Experimental Medicine, University of Palermo, Palermo, Italy
    Cancer Biol Ther 7:801-9. 2008
    ....
  92. ncbi Origin and evolution of eukaryotic chaperonins: phylogenetic evidence for ancient duplications in CCT genes
    J M Archibald
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    Mol Biol Evol 17:1456-66. 2000
    b>Chaperonins are oligomeric protein-folding complexes which are divided into two distantly related structural classes...
  93. ncbi Serum heat shock protein and anti-heat shock protein antibody levels in aging
    I M Rea
    Department of Geriatric Medicine, The Queen s University of Belfast, Whitla Medical Building, 97 Lisburn Road, Belfast, BT9 7BL, Northern Ireland, UK
    Exp Gerontol 36:341-52. 2001
    ..Further studies are required to understand the basis for declining serum Hsp60 and Hsp70 levels in aging and to elucidate their origin and role in the maintenance of homeostasis and resistance to environmental challenges...
  94. ncbi Different molecular methods for the identification of rarely isolated non-tuberculous mycobacteria and description of new hsp65 restriction fragment length polymorphism patterns
    Bernhard Häfner
    Department of Hygiene and Medical Microbiology, Hygiene Institute, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Mol Cell Probes 18:59-65. 2004
    ..Confusion may be caused by older data base entries which are not updated and not longer reflect the actual systematic and taxonomy of the genus Mycobacterium...
  95. ncbi Structure of an Hsp90-Cdc37-Cdk4 complex
    Cara K Vaughan
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, UK
    Mol Cell 23:697-707. 2006
    ..Comparison with the crystal structure of Hsp90 allows us to identify the locations of Cdc37 and Cdk4 in the complex and suggests a mechanism by which conformational changes in the kinase are coupled to the Hsp90 ATPase cycle...
  96. ncbi Characterization of archaeal group II chaperonin-ADP-metal fluoride complexes: implications that group II chaperonins operate as a "two-stroke engine"
    Ryo Iizuka
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    J Biol Chem 280:40375-83. 2005
    Group II chaperonins, found in Archaea and in the eukaryotic cytosol, act independently of a cofactor corresponding to GroES of group I chaperonins...
  97. ncbi Two families of chaperonin: physiology and mechanism
    Arthur L Horwich
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Annu Rev Cell Dev Biol 23:115-45. 2007
    b>Chaperonins are large ring assemblies that assist protein folding to the native state by binding nonnative proteins in their central cavities and then, upon binding ATP, release the substrate protein into a now-encapsulated cavity to ..
  98. ncbi Mycobacterium cosmeticum sp. nov., a novel rapidly growing species isolated from a cosmetic infection and from a nail salon
    Robert C Cooksey
    Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Int J Syst Evol Microbiol 54:2385-91. 2004
    ....
  99. ncbi Archaeal group II chaperonin mediates protein folding in the cis-cavity without a detachable GroES-like co-chaperonin
    Takao Yoshida
    Kamaishi Laboratories, Marine Biotechnology Institute Co Ltd, 3 75 1 Heita, Kamaishi, 026 0001, Iwate, Japan
    J Mol Biol 315:73-85. 2002
    Group II chaperonins of archaea and eukaryotes are distinct from group I chaperonins of bacteria. Whereas group I chaperonins require the co-chaperonin Cpn-10 or GroES for protein folding, no co-chaperonin has been known for group II...
  100. pmc How protein thermodynamics and folding mechanisms are altered by the chaperonin cage: molecular simulations
    Fumiko Takagi
    PRESTO, Japan Science and Technology Corporation, and Department of Chemistry, Faculty of Science, Kobe University, Rokkodai, Nada, Kobe 657 8501, Japan
    Proc Natl Acad Sci U S A 100:11367-72. 2003
    ..We also show how ensemble of folding pathways are altered by the chaperonin-like cage calculating a variant of value used in the study of spontaneous folding...
  101. ncbi Gene duplication and the evolution of group II chaperonins: implications for structure and function
    J M Archibald
    Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Struct Biol 135:157-69. 2001
    b>Chaperonins are multisubunit protein-folding assemblies. They are composed of two distinct structural classes, which also have a characteristic phylogenetic distribution...

Research Grants62

  1. Development of Chemical Chaperonin for Medium Chain Acyl-CoA Dehydrogenase Defici
    AL WALID ABDEL MOHSEN; Fiscal Year: 2010
    ..There are two specific aims. Specific Aim 1 is focused on the identification of chemical chaperonins using two random in vitro chemical library screening techniques, an HCS (high content-image based screening) ..
  2. Center for Protein Folding Machinery
    Vijay S Pande; Fiscal Year: 2013
    ..understanding of cellular protein folding nanomachines such as mammalian TRiC and the archaeal Mm-cpn chaperonins. Our objective is to re-engineer them for biomedical applications...
  3. 3 D ELECTRON MICROSCOPY OF MACROMOLECULES
    Wah Chiu; Fiscal Year: 2013
    ..and CCD cameras and accomplished de novo backbone traces of proteins in both viruses and molecular chaperonins, our Center is well poised to continue our research mission, including 4 core projects, 12 collaborative ..
  4. The Biophysics of Virus Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2013
    ..capsid of core protein (Cp) containing a reverse transcriptase-pregenomic RNA complex and associated chaperonins. We show that the dimeric Cp has a strong propensity to self-assemble in vitro and will bind any nucleic acid...
  5. 3 D ELECTRON MICROSCOPY OF MACROMOLECULES
    Wah Chiu; Fiscal Year: 2011
    ..and CCD cameras and accomplished de novo backbone traces of proteins in both viruses and molecular chaperonins, our Center is well poised to continue our research mission, including 4 core projects, 12 collaborative ..
  6. Post-translational control mechanisms of the circadian clock
    David E Somers; Fiscal Year: 2013
    ..The second is the effect chaperonins play in the maturation of the F-box protein ZEITLUPE to its functional state, necessary to the maintenance of ..
  7. Host Partners and Virus Assembly
    Adam Zlotnick; Fiscal Year: 2010
    ..The cores are comprised of a reverse transcriptase-pregenomic RNA complex and associated chaperonins contained in an icosahedral capsid of core protein (Cp)...
  8. Mechanism of the Eukaryotic Chaperonin in TRiC/CCT
    Judith Frydman; Fiscal Year: 2013
    ..b>Chaperonins are essential components of the cellular folding machinery...
  9. Mechanisms of the eukaryotic chaperonin TRiC/CCT and its cochaperone Prefoldin
    DAN GESTAUT; Fiscal Year: 2013
    ..While most chaperones are redundant in function, TRiC and its homologous chaperonins are essential in all kingdoms...
  10. FASEB CONFERENCE ON VIRAL ASSEMBLY
    Roger Burnett; Fiscal Year: 1992
    ..Some recent findings have included the role of chaperonins in structural protein maturation, phosphorylation of viral structural subunits, nonconservation of symmetry in ..
  11. MECHANISM OF CHAPERONE MEDIATED PROTEIN FOLDING
    Jonathan Weissman; Fiscal Year: 2000
    ..The present studies will focus on the so called "chaperonin" family of ring-shaped molecular chaperones. The chaperonins form a ubiquitous and abundant family of proteins whose members play an essential role in assisting folding in ..
  12. STRUCTURE AND DYNAMICS OF A MINI-CHAPERONE
    Qing xin Hua; Fiscal Year: 2003
    ..How chaperonins interact with and assist in the folding of nascent polypeptides represent problems of fundamental significance ..
  13. GENETIC ANALYSIS OF HEAT SHOCK PROTEIN FUNCTION
    Gregory Phillips; Fiscal Year: 1991
    ..These proteins belong to a highly evolutionarily conserved class of molecules called chaperonins, which catalyze the folding of polypeptide chains into their final conformations...
  14. CHAPERONIN AIDED PROTEIN FOLDING OF RHODANESE
    PAUL HOROWITZ; Fiscal Year: 2002
    ..Controlled release then leads to native conformations. Chaperonins respond to disease, and they are used diagnostically, e.g. for breast cancer...
  15. STRUCTURAL BASIS OF GROEL/SUBSTRATE INTERACTIONS
    JOHN ROSENBERG; Fiscal Year: 2000
    ..6 angstroms resolution. Chaperonins, including members of the Cpn60 family are a ubiquitous class of proteins that assist in protein folding in ..
  16. YEAST MITOCHONDRIAL PROTEASES
    Fred Sherman; Fiscal Year: 1993
    ..Possible heat-shock character of the enzyme will be tested and its functional relationship with mitochondrial chaperonins will be examined. ATP-independent protease(s) of yeast mitochondria will be also purified and characterized...
  17. FASEB CONFERENCE: VIRAL ASSEMBLY
    Jonathan King; Fiscal Year: 1990
    ..In targets for therapeutic intervention. Recent findings including the role of chaperonins in structural protein maturation, phosphorylation of viral structural protein maturation, phosphorylation of ..
  18. Dodecahedral Coordination-Cages as Abiological Chaperonins for Protein Folding.
    BRIAN NORTHROP; Fiscal Year: 2009
    The proposed studies seek to design and synthesize artificial chaperonins for the purpose of investigating the process of protein folding...
  19. MECHANISMS OF CYTOSOLIC CHAPERONIN ACTION
    JORG MARTIN; Fiscal Year: 2000
    ..addressed with the purified components in vitro: Folding assays with immobilized chaperonin complexes and trap chaperonins will be used in experiments to determine the mechanism of GroEL/GroES and TRiC action...
  20. Molecular Basis for Protein Interactions with a Biological Nanomachine
    RIINA TEHVER; Fiscal Year: 2009
    ..This research will clarify the inner workings of chaperonins and also add to our knowledge of protein-protein interactions in general...
  21. Center for Protein Folding Machinery (RMI)
    Wah Chiu; Fiscal Year: 2004
    ..We plan to take an integrated approach to study these chaperonins with the most suitable physical and computational methods including electron cryomicroscopy, single-molecule ..
  22. Structure and Function of Class II MHC Proteins
    Lawrence Stern; Fiscal Year: 2006
    ..involves in vitro biochemical and biophysical analysis of recombinant class II MHC proteins, associated chaperonins, and peptide exchange factors...
  23. Multi-Resolution Docking Methods for Electron Microscopy
    Willy Wriggers; Fiscal Year: 2009
    ..Collaborative efforts will include the refinement of myosin motors, GroEL chaperonins, and RNA polymerase assemblies...
  24. Single-Molecule Studies of Chaperonin Mechanism
    WILLIAM MOERNER; Fiscal Year: 2006
    ..Double-ring chaperonins bind unfolded polypeptides within their ring cavities, prevent off-pathway reactions, and promote productive ..
  25. GroEL: How Is Allostery Coupled to Assisted Folding?
    GEORGE LORIMER; Fiscal Year: 2007
    ..The chaperonins, ubiquitous and indispensable proteins, play an important role in vivo, assisting their substrate proteins (SP) ..
  26. CONFERENCE ON BIOLOGICAL REGULATORY MECHANISMS
    LUCIA ROTHMAN DENES; Fiscal Year: 1991
    ..A session will address the regulation of protein trafficking and targeting and the role of chaperonins in the process...
  27. Chaperonin-mediated protein folding
    Arthur Horwich; Fiscal Year: 2007
    ..concerning the mechanism of action of the essential megadalton-sized double ring molecular machines known as chaperonins, which mediate ATPdependent protein folding to the native state in a variety of cellular compartments...
  28. MECHANISM OF ACTION OF THE HSP100 CHAPERONE CLPA
    Arthur Horwich; Fiscal Year: 2006
    ....
  29. PROFILING SYNOVIAL VASCULATURE IN ARTHRITIS-PRONE RATS
    KAMAL MOUDGIL; Fiscal Year: 2005
    ..abstract_text> ..
  30. INTERFACIAL ACTIVATION OF WATER SOLUBLE PHOSPHOLIPASES
    Mary Roberts; Fiscal Year: 2003
    ..abstract_text> ..
  31. Modulation of Autoimmunity by Green Tea Polyphenols
    KAMAL MOUDGIL; Fiscal Year: 2006
    ..The results of this study would help in establishing a reliable scientific basis for the use of PGT in autoimmune arthritis, and in developing better therapeutic approaches for RA. ..
  32. A PROGRAM FOR RAT GENE DISCOVERY AND MAPPING
    Val Sheffield; Fiscal Year: 2001
    ..Progress during the initial phase of this project resulted in unprecedented efficiency in gene discovery. ..
  33. SELF-DETERMINANTS & INDIRECTION OF ANTI-SELF IMMUNITY
    KAMAL MOUDGIL; Fiscal Year: 2001
    ..They believe that such predetermined, experimentally programmed control of the display hierarchy of cryptic self determinants would pave the way for novel immunotherapeutic strategies for prevention and treatment of cancer. ..
  34. REGULATION OF AUTOIMMUNITY THROUGH EPITOPE SPREADING
    KAMAL MOUDGIL; Fiscal Year: 2006
    ..This in turn would contribute to developing novel therapeutic approaches for RA and other autoimmune diseases. [unreadable] [unreadable]..
  35. Molecular Genetics of Glaucoma
    Val C Sheffield; Fiscal Year: 2010
    ..Candidate genes for mutations screening will be identified using a combination of expression, functional and positional information. ..
  36. Biochemical Stabilization for Freeze Dried Platelets
    Christopher Wagner; Fiscal Year: 2003
    ..abstract_text> ..
  37. Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cells
    JAISRI LINGAPPA; Fiscal Year: 2008
    ..abstract_text> ..
  38. PROTEIN FOLDING IN THE EUKARYOTIC CYTOSOL
    Judith Frydman; Fiscal Year: 2010
    ..Finally, our fourth specific aim will explore the interaction between chaperones and the translational machinery. ..
  39. Refinement of Macromolecular Assembly Structure using Electron Microscopy
    Michael S Chapman; Fiscal Year: 2010
    ..A second goal is a more detailed mechanistic understanding of biomolecular complexes, going beyond domain movements to the internal conformational changes that drive them. ..
  40. AGE AND CATARACT RELATED CHANGES IN LENS PROTEINS
    Jack Liang; Fiscal Year: 2002
    ..The knowledge obtained from this proposal may provide insight on cataract formation on a molecular level and may help in formulating a strategy for developing anticataract agents. ..
  41. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  42. PHOSPAGEN KINASE STRUCTURE, MECHANISM AND SPECIFICITY
    Michael Chapman; Fiscal Year: 2002
    ....
  43. Conference: Diffraction Methods in Structural Biology
    Michael Chapman; Fiscal Year: 2002
    ..Junior participation will be encouraged with a new policy of 3 mini-talks each day invited from the poster-presentations by the day's session chairs. ..
  44. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  45. NATURAL HISTORY OF ANAL NEOPLASIA IN HIV INFECTED MEN
    Joel Palefsky; Fiscal Year: 2002
    ....
  46. GENE EXPRESSION BY HUMAN GRANULOCYTIC EHRLICHIOSIS AG
    Yasuko Rikihisa; Fiscal Year: 2004
    ..These efforts are expected to reveal an important role for the p44-multigene family in strategies of adaptive surface variation and avoidance of host immune responses. ..
  47. Elucidating the Function of Novel Epigenetic Element
    HEATHER TRUE KROB; Fiscal Year: 2005
    ....
  48. Gene Expression Levels Across Diverse Genomes
    Samuel Karlin; Fiscal Year: 2006
    ..A third major objective of our research will be to extend our codon usage methods for predicting gene expression levels to eukaryotic genomes, including yeast, D. melanogaster, C. elegans, and human. [unreadable] [unreadable]..
  49. BIOGENESIS OF THE YEAST MITOCHONDRIAL ATPASE COMPLEX
    Sharon Ackerman; Fiscal Year: 2006
    ....
  50. ANDROGEN RECEPTOR DEGRADATION
    Avrom Caplan; Fiscal Year: 2006
    ..abstract_text> ..
  51. Virulence and Immunity of Granulocytic Ehrlichiae
    JOHN DUMLER; Fiscal Year: 2005
    ..This information will provide the framework for rational design of new therapies aimed at modulating immune mechanisms and design of preventative measures aimed at immunization. ..
  52. Cell-free VEE assembly system and alphavirus drug screen
    JAISRI LINGAPPA; Fiscal Year: 2006
    ..The cell-free VEE assembly system offer a safe and low cost approach to understanding the mechanism of VEE capsid formation and to screen for agents active against alphaviruses that have potential as bioterrorism agents. ..
  53. A Chaperonin/Osmolyte Protein Folding Screen - STTR Phase I
    Mark Fisher; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  54. MOLECULAR MECHANISMS OF BACTERIAL PATHOGENESIS
    Robert Collier; Fiscal Year: 2007
    ..abstract_text> ..
  55. AUA/SBUR Res. Conf.-"Inflammation in Prostate Diseases"
    William Nelson; Fiscal Year: 2005
    ..Support for this meeting will encourage the future development of urology research by providing mentoring for new investigators as well as education on this emerging research area. ..
  56. Anal HPV Infection and ASIL in HIV+ and HIV- Women
    Joel Palefsky; Fiscal Year: 2006
    ..Knowledge of genes involved in ASIL pathogenesis may provide new approaches to therapy of both anal and cervical HSIL as well as markers for disease progression. ..
  57. Immunoglobulin Allotypes in Hepatitis C Virus Infection
    JANARDAN PANDEY; Fiscal Year: 2008
    ..Results of this investigation will advance our understanding of the role of host genetic factors in clearance and persistence of hepatitis C virus infection. ..
  58. REGULATION AND FUNCTION OF THE YEAST HEAT SHOCK RESPONSE
    Elizabeth Craig; Fiscal Year: 2008
    ..In addition, we will determine the sequence of interactions of a nascent chain with the ribosome-associated chaperones. ..
  59. MBD2 as a Target for Cancer Prevention and Treatment
    William Nelson; Fiscal Year: 2007
    ..abstract_text> ..
  60. Mapping interaction surface domains in lens crystallins
    Jack Liang; Fiscal Year: 2008
    ..The long-term objective is to understand the mechanisms of dimerization and oligomerization, and the results may help us to understand the function of alpha-crystallin in normal lens and in cataract formation. ..
  61. Hsp90 Chaperone Machine Structure and Function
    Avrom Caplan; Fiscal Year: 2009
    ..In the second sub-aim we will assay for chaperone activity of novel co-chaperones that are important for protein kinase folding. Finally, we will distinguish between the role of Cdc37 in Cdk folding and cyclin binding. ..