ras grf1

Summary

Summary: A guanine nucleotide exchange factor that is expressed primarily in neuronal tissue and may be specific for the Ha-ras homolog of the RAS PROTEINS.

Top Publications

  1. ncbi The cdc25 protein contains an intrinsic phosphatase activity
    W G Dunphy
    Division of Biology, California Institute of Technology, Pasadena 91125
    Cell 67:189-96. 1991
  2. pmc Phosphotyrosine (p-Tyr)-dependent and -independent mechanisms of p190 RhoGAP-p120 RasGAP interaction: Tyr 1105 of p190, a substrate for c-Src, is the sole p-Tyr mediator of complex formation
    R W Roof
    Department of Microbiology and Cancer Center, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 18:7052-63. 1998
  3. pmc The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe
    K Tanaka
    School of Biological Sciences, 2 205 Stopford Building, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK
    EMBO J 20:1259-70. 2001
  4. pmc Aberrant Ras regulation and reduced p190 tyrosine phosphorylation in cells lacking p120-Gap
    P van der Geer
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Mol Cell Biol 17:1840-7. 1997
  5. ncbi Integration of calcium and Ras signalling
    Peter J Cullen
    The Henry Wellcome Laboratories for Integrated Cell Signalling, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 3:339-48. 2002
  6. ncbi The N-terminal moiety of CDC25(Mm), a GDP/GTP exchange factor of Ras proteins, controls the activity of the catalytic domain. Modulation by calmodulin and calpain
    S Baouz
    Groupe de Biophysique Equipe 2, Ecole Polytechnique, F 91128 Palaiseau cedex, France
    J Biol Chem 272:6671-6. 1997
  7. ncbi Wee1-dependent mechanisms required for coordination of cell growth and cell division
    Douglas R Kellogg
    Sinsheimer Laboratories, Department of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, CA 95064, USA
    J Cell Sci 116:4883-90. 2003
  8. ncbi GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors
    Kent L Rossman
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Mol Cell Biol 6:167-80. 2005
  9. ncbi Filamin A-mediated down-regulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cells
    Tie Nian Zhu
    Diabetes Section, Laboratory of Clinical Investigation, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 282:14816-26. 2007
  10. pmc G protein beta gamma subunit-dependent Rac-guanine nucleotide exchange activity of Ras-GRF1/CDC25(Mm)
    M Kiyono
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Proc Natl Acad Sci U S A 96:4826-31. 1999

Scientific Experts

Detail Information

Publications180 found, 100 shown here

  1. ncbi The cdc25 protein contains an intrinsic phosphatase activity
    W G Dunphy
    Division of Biology, California Institute of Technology, Pasadena 91125
    Cell 67:189-96. 1991
    ..These observations indicate that the cdc25 protein can function as a tyrosine phosphatase in the absence of any other protein...
  2. pmc Phosphotyrosine (p-Tyr)-dependent and -independent mechanisms of p190 RhoGAP-p120 RasGAP interaction: Tyr 1105 of p190, a substrate for c-Src, is the sole p-Tyr mediator of complex formation
    R W Roof
    Department of Microbiology and Cancer Center, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 18:7052-63. 1998
    ..These studies describe a specific mechanism by which c-Src can regulate p190-p120 association and also document a significant role for p-Tyr-independent means of p190-p120 binding...
  3. pmc The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe
    K Tanaka
    School of Biological Sciences, 2 205 Stopford Building, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK
    EMBO J 20:1259-70. 2001
    ..The ability of plo1(+) overexpression to induce septation was severely compromised by SIN inactivation. We propose that Plo1 acts before the SIN to control septation...
  4. pmc Aberrant Ras regulation and reduced p190 tyrosine phosphorylation in cells lacking p120-Gap
    P van der Geer
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Mol Cell Biol 17:1840-7. 1997
    ....
  5. ncbi Integration of calcium and Ras signalling
    Peter J Cullen
    The Henry Wellcome Laboratories for Integrated Cell Signalling, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 3:339-48. 2002
    ..Here, we focus on examining the link between calcium and Ras signalling and, in particular, we speculate as to how the complexity of calcium signalling could regulate Ras activity...
  6. ncbi The N-terminal moiety of CDC25(Mm), a GDP/GTP exchange factor of Ras proteins, controls the activity of the catalytic domain. Modulation by calmodulin and calpain
    S Baouz
    Groupe de Biophysique Equipe 2, Ecole Polytechnique, F 91128 Palaiseau cedex, France
    J Biol Chem 272:6671-6. 1997
    ..Together, these results emphasize the role of the other domains of CDC25(Mm) in controlling the activity of the catalytic domain and support the involvement of calmodulin and calpain in the in vivo regulation of the CDC25(Mm) activity...
  7. ncbi Wee1-dependent mechanisms required for coordination of cell growth and cell division
    Douglas R Kellogg
    Sinsheimer Laboratories, Department of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, CA 95064, USA
    J Cell Sci 116:4883-90. 2003
    ..Further understanding of these signaling networks may provide important clues to how cell growth and cell division are coordinated...
  8. ncbi GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors
    Kent L Rossman
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Mol Cell Biol 6:167-80. 2005
    ..The failure to do so can have significant consequences and is reflected in the aberrant function of Dbl-family GEFs in some human diseases...
  9. ncbi Filamin A-mediated down-regulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cells
    Tie Nian Zhu
    Diabetes Section, Laboratory of Clinical Investigation, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 282:14816-26. 2007
    ..Our results indicate that expression of FLNa regulates constitutive activation of the Ras/ERK pathway partly through a Ras-GRF1 mechanism to modulate the production of MMP-9...
  10. pmc G protein beta gamma subunit-dependent Rac-guanine nucleotide exchange activity of Ras-GRF1/CDC25(Mm)
    M Kiyono
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Proc Natl Acad Sci U S A 96:4826-31. 1999
    ..These results suggest a role of Ras-GRF1 for regulating Rac-dependent as well as Ras-dependent signaling pathways, particularly in the brain functions...
  11. pmc Regulation of DNA methylation of Rasgrf1
    Bong June Yoon
    Dept of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York 14263, USA
    Nat Genet 30:92-6. 2002
    ..In addition, DMD methylation is required for imprinted Rasgrf1 expression. Together the DMD and repeat element constitute a binary switch that regulates imprinting at the locus...
  12. pmc Loss of a protein phosphatase 2A regulatory subunit (Cdc55p) elicits improper regulation of Swe1p degradation
    H Yang
    Department of Biology, Syracuse University, Syracuse, New York 13244, USA
    Mol Cell Biol 20:8143-56. 2000
    ..We also present evidence indicating that, in cdc55-null cells, misregulated PP2A phosphatase activity is the cause of both the ectopic stabilization of Swe1p and the production of the morphologically abnormal phenotype...
  13. pmc Timing and sequence requirements defined for embryonic maintenance of imprinted DNA methylation at Rasgrf1
    Rebecca Holmes
    Division of Nutritional Sciences, College of Agricultural and Life Sciences, Cornell University, Nutritional Sciences, Ithaca, NY 14853, USA
    Mol Cell Biol 26:9564-70. 2006
    ..Our data show that the Rasgrf1 repeats serve at least two functions: first, to establish Rasgrf1 DNA methylation in the male germ line, and second, to resist global demethylation in the preimplantation embryo...
  14. pmc Fission yeast Clp1p phosphatase affects G2/M transition and mitotic exit through Cdc25p inactivation
    Benjamin A Wolfe
    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232, USA
    EMBO J 23:919-29. 2004
    ..This may be a widely conserved mechanism whereby Cdc14 proteins contribute to Cdk1p inactivation...
  15. pmc A yeast-based growth assay for the analysis of site-specific proteases
    Fabian Kohler
    Institut fur Biologie III, Albert Ludwigs Universitat, Schanzlestrasse 1, 79104 Freiburg, Germany
    Nucleic Acids Res 31:e16. 2003
    ..It has significant potential for the selection of inhibitors of cytoplasmic and membrane-associated proteases of biotechnical and clinical relevance...
  16. ncbi Analysis of interaction between Ras and CDC25 guanine nucleotide exchange factor using yeast GAL4 two-hybrid system
    R D Mosteller
    Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, University of Southern California School of Medicine, Los Angeles 90033, USA
    Methods Enzymol 255:135-48. 1995
    ..We recommend that the two-hybrid system be employed in combination with other approaches, including molecular genetic analyses and in vitro binding experiments, for the study of Ras and CDC25-GEF interactions...
  17. pmc Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe
    Fiona H MacIver
    Paterson Institute for Cancer Research, Manchester M20 4BX, UK
    Genes Dev 17:1507-23. 2003
    ..s11 suppresses cdc25.22 by promoting Plo1 activity. Furthermore, the delayed mitotic commitment of plo1.ts2 cells suggests that Plo1 is an integral part of the core controls that modulate MPF activation in S. pombe...
  18. ncbi Characterization of Saccharomyces cerevisiae Ras1p and chimaeric constructs of Ras proteins reveals the hypervariable region and farnesylation as critical elements in the adenylyl cyclase signaling pathway
    Jean Bernard Créchet
    Groupe RMN, I C S N, Ecole Polytechnique, F 91128 Palaiseau cedex, France
    Biochemistry 42:14903-12. 2003
    ..This strongly suggests that the hypervariable region of Ras proteins can interact directly with adenylyl cyclase...
  19. ncbi Analysis of the role of the hypervariable region of yeast Ras2p and its farnesylation in the interaction with exchange factors and adenylyl cyclase
    J B Crechet
    Groupe de Biophysique Equipe 2, Ecole Polytechnique, F 91128 Palaiseau cedex, France
    J Biol Chem 275:17754-61. 2000
    ..The use of Ha-Ras/Ras2p chimaeras of different length emphasized the key role of the hypervariable region of Ras2p in inducing maximum activation of adenylyl cyclase and for a productive interaction with membrane-bound GEF...
  20. ncbi HIV-1 Vpr induces cell cycle G2 arrest in fission yeast (Schizosaccharomyces pombe) through a pathway involving regulatory and catalytic subunits of PP2A and acting on both Wee1 and Cdc25
    R T Elder
    Children's Memorial Institute for Education and Research, Children's Memorial Hospital, Chicago, Illinois 60614, USA
    Virology 287:359-70. 2001
    ..These two genes may be part of the uncharacterized pathway for Vpr-induced G2 arrest in which Vpr upregulates PP2A to activate Wee1 and inhibit Cdc25...
  21. ncbi Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway
    P Chaturvedi
    Department of Oncology Research, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA
    Oncogene 18:4047-54. 1999
    ..Activation of Chk2 might not only delay mitotic entry, but also increase the capacity of cultured cells to survive after treatment with gamma-radiation or with the topoisomerase-I inhibitor topotecan...
  22. ncbi Ssa1p chaperone interacts with the guanine nucleotide exchange factor of ras Cdc25p and controls the cAMP pathway in Saccharomyces cerevisiae
    M Geymonat
    Laboratoire Information Génétique et Développement, Institut de Genetique et Microbiologie, UMR CNRS Université 2225, Universite Paris Sud, Orsay, France
    Mol Microbiol 30:855-64. 1998
    ..Altogether, these results lead to the conclusion that Ssa1p positively controls the cAMP pathway through Cdc25p. We propose that this connection plays a critical role in the adaptation of cells to stress conditions...
  23. ncbi Yeast growth selection system for detecting activity and inhibition of dimerization-dependent receptor tyrosine kinase
    Tea Gunde
    ESBATech AG, Zurich Schlieren, Switzerland
    Biotechniques 39:541-9. 2005
    ..The RTK cellular assay presented here can be applied in high-throughput screens for selecting RTK-specific inhibitors that must be able to permeate the membrane and to function in an eukaryotic intrecellular environment...
  24. pmc A novel approach for the identification of protein-protein interaction with integral membrane proteins
    M Hubsman
    Department of Molecular Genetics and the Rappaport Family Institute for Research in the Medical Sciences and the B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, PO Box 9649, Bat-Galim, Haifa 31096, Israel
    Nucleic Acids Res 29:E18. 2001
    ..The system serves as an attractive approach to explore novel protein-protein interactions with high specificity and selectivity, where other methods fail...
  25. ncbi Nuclear localization of Cdc25 is regulated by DNA damage and a 14-3-3 protein
    A Lopez-Girona
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 397:172-5. 1999
    ..Thus, Rad24 appears to function as an attachable nuclear-export signal that enhances the nuclear export of Cdc25 in response to DNA damage...
  26. pmc Suppressors of cdc25p overexpression identify two pathways that influence the G2/M checkpoint in fission yeast
    K C Forbes
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 150:1361-75. 1998
    ..These results suggest that chk1(+) and rad24(+) may function redundantly with cds1(+) in the checkpoint response to unreplicated DNA...
  27. pmc Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae
    S Colombo
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Flanders, Belgium
    EMBO J 17:3326-41. 1998
    ..Interaction of Ras2(val19) with adenylate cyclase apparently prevents its activation by both agonists...
  28. pmc Roles of the mitotic inhibitors Wee1 and Mik1 in the G(2) DNA damage and replication checkpoints
    N Rhind
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell Biol 21:1499-508. 2001
    ..Mik1 appears to have two roles in the DNA damage checkpoint; one in the establishment of the checkpoint and another in its maintenance. In contrast, Wee1 does not appear to be involved in the establishment of either checkpoint...
  29. pmc Mcs4, a two-component system response regulator homologue, regulates the Schizosaccharomyces pombe cell cycle control
    G Cottarel
    Mitotix, Cambridge, Massachusetts 02139, USA
    Genetics 147:1043-51. 1997
    ..These findings link the two-component signal transduction system to stress response and cell cycle control in S. pombe...
  30. ncbi Dimerization of Cdc25p, the guanine-nucleotide exchange factor for Ras from Saccharomyces cerevisiae, and its interaction with Sdc25p
    C Camus
    Laboratoire Information Génétique et Développement, Institut de Genetique et Microbiologie, URA CNRS 2225, Universite Paris Sud, France
    Eur J Biochem 247:703-8. 1997
    ..The biological relevance of dimerization is still an open question, however by allowing heterodimerization with Sdc25p it could permit a more complex combinatorial regulation of ras in yeast...
  31. ncbi Ras2 and Ras1 protein phosphorylation in Saccharomyces cerevisiae
    J L Whistler
    Division of Genetics, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    J Biol Chem 272:18790-800. 1997
    ..This site, when mutated to alanine, led to promiscuous phosphorylation of Ras2 protein on nearby serine residues. A decrease in phosphorylation may lead to a decrease in signaling through the Ras-cAMP pathway...
  32. ncbi Nim1 kinase promotes mitosis by inactivating Wee1 tyrosine kinase
    L Wu
    Department of Molecular Biology, Scripps Research Institute, La Jolla 92037
    Nature 363:738-41. 1993
    ..Highly purified Nim1 kinase phosphorylates Wee1 in vitro, resulting in strong inhibition of Wee1 kinase. These observations show that Nim1 promotes the onset of mitosis by inhibiting Wee1...
  33. ncbi Human Raf-1 proteins associate with Rad24 and Cdc25 in cell-cycle checkpoint pathway of fission yeast, Schizosaccharomyces pombe
    Michael Lee
    Department of Biology, College of Natural Sciences, University of Incheon, Incheon, Korea
    J Cell Biochem 101:488-97. 2007
    ..Thus, these data suggest that Raf-1 may interfere with the role of Rad24 by competing with Rad24 for binding to Cdc25 or a direct phosphorylation of Cdc25, bypassing the checkpoint pathway in DNA repair through Cdc25 activation...
  34. pmc Mei4p coordinates the onset of meiosis I by regulating cdc25+ in fission yeast
    Yuko Murakami-Tonami
    Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya 467 8601, Japan
    Proc Natl Acad Sci U S A 104:14688-93. 2007
    ..We propose that Mei4p acts as a rate-limiting regulator of meiosis I by activating cdc25(+) transcription in coordination with other meiotic events...
  35. pmc Human immunodeficiency virus type 1 Vpr induces cell cycle G2 arrest through Srk1/MK2-mediated phosphorylation of Cdc25
    Sylvain Huard
    Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, MSTF700A, Baltimore, MD 21201, USA
    J Virol 82:2904-17. 2008
    ..Collectively, our data suggest that Vpr induces cell cycle G(2) arrest at least in part through a Srk1/MK2-mediated mechanism...
  36. ncbi Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase
    K L Gould
    Department of Biochemistry, University of Oxford, United Kingdom
    Science 250:1573-6. 1990
    ..The complementary DNA that encoded the tyrosine phosphatase replaced the mitotic activator p80cdc25, closely associating the cdc25(+)-activating pathway with tyrosine dephosphorylation of pp34...
  37. ncbi An additional homolog of the fission yeast cdc25+ gene occurs in humans and is highly expressed in some cancer cells
    A Nagata
    Department of Molecular Genetics, Osaka University, Japan
    New Biol 3:959-68. 1991
    ..Thus, at least two homologs of the cdc25 gene exist and are both expressed in human cells. The implications of CDC25Hu2 overexpression in some cancer cells are discussed...
  38. ncbi In vitro interaction between Saccharomyces cerevisiae CDC25 and RAS2 proteins
    M D Baroni
    Dipartimento di Fisiologia e Biochimica Generali, Universita degli Studi di Milano, Italy
    Biochem Biophys Res Commun 186:467-74. 1992
    ..The 310 C-terminal aminoacids of CDC25 bind RAS2 while a C-terminus deletion within this aminoacid stretch abolishes the binding. The possible physiological significance of these findings is discussed...
  39. ncbi MAPK mediated cell cycle regulation is associated with Cdc25 turnover in S. pombe after exposure to genotoxic stress
    Geetanjali Sundaram
    B C Guha Centre for Genetic Engineering and Biotechnology, Department of Biochemistry, University of Calcutta, Kolkata, India
    Cell Cycle 7:365-72. 2008
    ..We also show that Sty1 activity is not essential for activation of the S phase checkpoint...
  40. pmc The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo
    T Munder
    Department of Biotechnology, Ciba Geigy Ltd, Basel, Switzerland
    Mol Cell Biol 12:2091-9. 1992
    ..Cdc25 binds predominantly to the catalytically inactive GDP-bound form of Ras2, whereas a conformational change of Ras2 to its activated GTP-bound state results in its loss of binding affinity to Cdc25...
  41. pmc Pyp3 PTPase acts as a mitotic inducer in fission yeast
    J B Millar
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037
    EMBO J 11:4933-41. 1992
    ..Pyp3 PTPase effectively dephosphorylates and activates the p34cdc2 kinase in vitro. We conclude that the pyp3 PTPase acts cooperatively with p80cdc25 to dephosphorylate Tyr15 of p34cdc2...
  42. pmc Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1
    Gayatri Pal
    Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Cell Biol 180:931-45. 2008
    ..Because casein kinase 1 is associated with sites of polar growth, it may regulate Mih1 as part of a signaling mechanism that links successful completion of growth-related events to cell cycle progression...
  43. ncbi The Flp1/Clp1 phosphatase cooperates with HECT-type Pub1/2 protein-ubiquitin ligases in Schizosaccharomyces pombe
    Verónica Esteban
    Centro de Investigacion del Cancer, Departamento de Microbiologia y Genetica, Universidad de Salamanca CSIC, Salamanca, Spain
    Cell Cycle 7:1269-76. 2008
    ..pombe cells. Thus Flp1p appears to have an important role in integrating HECT- and RING-type ubiquitin ligases in cell cycle control...
  44. pmc Cloning by functional complementation of a mouse cDNA encoding a homologue of CDC25, a Saccharomyces cerevisiae RAS activator
    E Martegani
    Dipartimento di Fisiologia e Biochimica Generali, Universita degli Studi di Milano, Italy
    EMBO J 11:2151-7. 1992
    ..Northern blot analysis of mouse brain poly(A)+ RNA reveals two major transcripts of approximately 1700 and 5200 nucleotides. Transcripts were found also in mouse heart and at a lower level in liver and spleen...
  45. ncbi The large N-terminal domain of Cdc25 protein of the yeast Saccharomyces cerevisiae is required for glucose-induced Ras2 activation
    Chiara Paiardi
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Milan, Italy
    FEMS Yeast Res 7:1270-5. 2007
    ....
  46. ncbi The glucose-induced polyphosphoinositides turnover in Saccharomyces cerevisiae is not dependent on the CDC25-RAS mediated signal transduction pathway
    G Frascotti
    Dipartimento di Fisiologia e Biochimica Generali, , Italy
    FEBS Lett 274:19-22. 1990
    ....
  47. ncbi Phosphorylation of the S. cerevisiae Cdc25 in response to glucose results in its dissociation from Ras
    E Gross
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Nature 360:762-5. 1992
    ..These results are of general significance because of the highly conserved sequence of Ras-guanyl nucleotide exchange factors from yeasts to mammals...
  48. ncbi Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p
    Daisuke Izawa
    Department of Biophysics and Biochemistry, Graduate School of Science, Tokyo 113 0033, Japan
    Nature 434:529-33. 2005
    ..We therefore propose that Mes1p has a key function in saving a sufficient level of MPF (M-phase-promoting factor) activity required for the execution of MII...
  49. ncbi Cdc25p, the guanine nucleotide exchange factor for the Ras proteins of Saccharomyces cerevisiae, promotes exchange by stabilizing Ras in a nucleotide-free state
    S A Haney
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544 1014
    J Biol Chem 269:16541-8. 1994
    ..These data, in conjunction with the kinetic data, are consistent with the hypothesis that Cdc25p catalyzes guanine nucleotide exchange by stabilizing a nucleotide-free intermediate of Ras...
  50. ncbi Mutagenic alteration of the distal switch II region of RAS blocks CDC25-dependent signaling functions
    M G Mirisola
    Dipartimento di Biologia Cellulare e dello Sviluppo, Centro di Oncobiologia Sperimentale, Palermo, Italy
    J Biol Chem 269:15740-8. 1994
    ..Wild-type, but not mutagenically altered, RAS2 proteins were stimulated by exchange factors in vitro. We conclude that the conserved distal switch II region is required for CDC25-dependent activation of RAS...
  51. ncbi Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression
    Cezar Stoica
    Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 281:37646-51. 2006
    ..We hypothesize that 14-3-3 proteins are required for Argonaute protein functions in cell cycle and/or gene-silencing pathways...
  52. ncbi A Drosophila gene encoding a DEAD box RNA helicase can suppress loss of wee1/mik1 function in Schizosaccharomyces pombe
    E Warbrick
    Department of Anatomy and Physiology, University of Dundee, Scotland
    Mol Gen Genet 245:654-7. 1994
    ..It is possible that the RNA helicase described here may regulate entry into mitosis by down regulating the expression of other genes whose activity may be rate-limiting for entry into mitosis...
  53. ncbi Identification of a human guanine nucleotide-releasing factor (H-GRF55) specific for Ras proteins
    F Schweighoffer
    Rhone Poulenc Rorer, Centre de Recherche de Vitry Alfortville, Vitry sur Seine, France
    Oncogene 8:1477-85. 1993
    ..Finally, in situ hybridization on of human chromosomes revealed a localization on band 15q2.4...
  54. ncbi Regulation of p34cdc2 protein kinase during mitosis
    S Moreno
    Department of Biochemistry University of Oxford, England
    Cell 58:361-72. 1989
    ..Therefore, the cdc25+, cdc13+, and suc1+ gene products are important for regulating p34cdc2 protein kinase activity during entry into, progress through, and exit from mitosis...
  55. pmc Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae
    S Powers
    Cold Spring Harbor Laboratory, New York 11724
    Mol Cell Biol 9:390-5. 1989
    ..These results suggest that these mutant RAS genes interfere with the normal interaction of RAS and CDC25 proteins and suggest that this interaction is direct and has evolutionarily conserved features...
  56. ncbi Properties and regulation of the catalytic domain of Ira2p, a Saccharomyces cerevisiae GTPase-activating protein of Ras2p
    M C Parrini
    Groupe de Biophysique, Ecole Polytechnique, Palaiseau, France
    Biochemistry 34:13776-83. 1995
    ..abstract truncated at 250 words)..
  57. ncbi [Isoprenylated proteins and cell proliferation: regulators and effectors of Ras proteins]
    J de Gunzburg
    INSERM U 248, Institut Curie, Paris
    C R Seances Soc Biol Fil 191:195-210. 1997
    ..Recent evidence, using effector domain mutants of Ras, demonstrates that these pathways cooperate to elicit the growth promoting effects of Ras proteins...
  58. ncbi Point mutation in the fibroblast growth factor receptor eliminates phosphatidylinositol hydrolysis without affecting neuronal differentiation of PC12 cells
    T Spivak-Kroizman
    Department of Pharmacology, New York University Medical Center, New York 10016
    J Biol Chem 269:14419-23. 1994
    ..Moreover, the aFGF-induced Ras signaling pathway, which is essential for PC12 cell differentiation, is not affected by elimination of PI hydrolysis...
  59. ncbi Isolation and characterization of the Pin1/Ess1p homologue in Schizosaccharomyces pombe
    H K Huang
    Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    J Cell Sci 114:3779-88. 2001
    ..Our genetic data also suggest that Pin1p might function as a positive regulator of Cdc25p and Wee1p...
  60. pmc A novel insight into aging: are there pluripotent very small embryonic-like stem cells (VSELs) in adult tissues overtime depleted in an Igf-1-dependent manner?
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Aging (Albany NY) 2:875-83. 2010
    ..Our preliminary data in long-living Igf-1-signaling-deficient mice show that these animals possess a 3-4 times higher number of VSELs deposited in adult BM, lending support to this novel hypothesis...
  61. ncbi Molecular characterization of isolated from murine adult tissues very small embryonic/epiblast like stem cells (VSELs)
    Dong Myung Shin
    Stem Cell Institute, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
    Mol Cells 29:533-8. 2010
    ..Mounting evidence accumulates that perturbation of expression of imprinted genes is a common phenomenon observed in developing tumors...
  62. ncbi Stimulation of Ras guanine nucleotide exchange activity of Ras-GRF1/CDC25(Mm) upon tyrosine phosphorylation by the Cdc42-regulated kinase ACK1
    M Kiyono
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 275:29788-93. 2000
    ..These results implicate ACK1 as an upstream modulator of Ras-GRF1 and suggest a signaling cascade consisting of Cdc42, ACK1, Ras-GRF1, and Ras in neuronal cells...
  63. ncbi Differential roles of NMDA Receptor Subtypes NR2A and NR2B in dendritic branch development and requirement of RasGRF1
    Fernando J Sepulveda
    Dept of Physiopathology, Faculty of Biological Sciences, Univ of Concepción, Concepcion, Chile
    J Neurophysiol 103:1758-70. 2010
    ..The dominated NR2A expression and the limited interactions of this subunit with the signaling protein RasGRF1 may contribute to the restricted dendritic arbor development in the adult CNS...
  64. ncbi Regulation of mitogen-activated protein kinases by glutamate receptors
    John Q Wang
    Department of Basic Medical Science, University of Missouri Kansas City, School of Medicine, Kansas City, Missouri, USA
    J Neurochem 100:1-11. 2007
    ....
  65. ncbi Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse
    Yuzuru Kato
    Department of Integrated Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan
    Hum Mol Genet 16:2272-80. 2007
    ..Finally, all these sequences showed moderate to severe hypomethylation in Dnmt3L-deficient prospermatogonia, indicating the critical function and broad specificity of this factor in de novo methylation...
  66. ncbi A phylogenetic approach to test for evidence of parental conflict or gene duplications associated with protein-encoding imprinted orthologous genes in placental mammals
    Mary J O'Connell
    Genetics and Biotechnology Lab, Department of Biochemistry and Biosciences Institute, University College Cork UCC, Lee Maltings 2 10, Cork, Ireland
    Mamm Genome 21:486-98. 2010
    ....
  67. ncbi Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning
    A M Chan
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Oncogene 12:1259-66. 1996
    ..The 3.0 and 2.4 kilobasepair (kb) transcripts of the NET1 gene was ubiquitously expressed in all tissues examined. Using fluorescence in situ hybridization, we localized the NET1 gene to the short arm of human chromosome 10 at band p15...
  68. ncbi Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae
    Dong Jian
    Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Nankai District, Tianjin, PR China
    FEBS Lett 584:4745-50. 2010
    ..These data suggest a negative feedback mechanism by which intracellular cAMP synthesis is inhibited by PKA through Cdc25p phosphorylation...
  69. ncbi A simple time delay model for eukaryotic cell cycle
    J Srividhya
    Indiana University School of Informatics, Indiana University, Bloomington, IN 47406, USA
    J Theor Biol 241:617-27. 2006
    ..Stochasticity has been introduced in the model to simulate the dependence of the cycle time on cell birth length. Mutant phenotypes in the stochastic model reproduce the experimental observations better than the deterministic model...
  70. pmc Novel epigenetic mechanisms that control pluripotency and quiescence of adult bone marrow-derived Oct4(+) very small embryonic-like stem cells
    D M Shin
    Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
    Leukemia 23:2042-51. 2009
    ....
  71. ncbi Small GTPase Ras and Rho expression in rat osteoblasts during spaceflight
    Yasuhiro Kumei
    Biochemistry, Department of Hard Tissue Engineering, Graduate School of Tokyo Medical and Dental University, Tokyo 113 8549, Japan
    Ann N Y Acad Sci 1095:292-9. 2007
    ..Microgravity signals may involve two pathways (G protein-coupled receptor-mediated pathway and tyrosine phosphorylation-mediated pathway) that activate Ras, Raf, and MAPK cascades in rat osteoblasts...
  72. ncbi Protein phosphatase 2A-negative regulation of the protective signaling pathway of Ca2+/CaM-dependent ERK activation in cerebral ischemia
    Jie Zhao
    Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, People s Republic of China
    J Neurosci Res 86:2733-45. 2008
    ....
  73. ncbi The effect of resveratrol on a cell model of human aging
    Maurizio Stefani
    Basic and Clinical Genomics Laboratory, School of Medical Sciences and Bosch Institute, The University of Sydney, Sydney, New South Wales 2006, Australia
    Ann N Y Acad Sci 1114:407-18. 2007
    ..Serum deprivation and high confluency caused nuclear translocation of the SIRT1-regulated transcription factor FOXO3a. Our data indicate resveratrol's actions might cause FOXO recruitment to the nucleus...
  74. pmc The 'RASor's' edge: Ras proteins and matrix destruction in arthritis
    Adam C Schmucker
    Department of Biochemistry, Dartmouth Medical School, North College Street, 7200 Vail Building, Hanover NH 03755, USA
    Arthritis Res Ther 11:136. 2009
    ..They elucidate a potential mechanism of regulation of MMP-3 expression in RA, suggesting a potential target for RA treatment...
  75. ncbi Epac- and Rap- independent ERK1/2 phosphorylation induced by Gs-coupled receptor stimulation in HEK293 cells
    Jens Henrik Norum
    Department of Pharmacology, University of Oslo, N 0316 Oslo, Norway
    FEBS Lett 581:15-20. 2007
    ..Different signalling pathways seem to be utilised by G(s)-coupled receptors in various isolates of HEK293 cells...
  76. pmc A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization
    N L Freeman
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Mol Cell Biol 16:548-56. 1996
    ..These observations identify CAP as an SH3 domain-binding protein and suggest that CAP mediates interactions between SH3 domain proteins and monomeric actin...
  77. pmc A non-coding RNA within the Rasgrf1 locus in mouse is imprinted and regulated by its homologous chromosome in trans
    Chelsea M Brideau
    Division of Nutritional Sciences, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 5:e13784. 2010
    ..Like many imprinted genes, Rasgrf1 lies within an imprinted cluster. One of four noncoding transcripts in the cluster, AK015891, is known to be imprinted...
  78. ncbi Simvastatin promotes osteoblast viability and differentiation via Ras/Smad/Erk/BMP-2 signaling pathway
    Pei Yu Chen
    Graduate Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
    Nutr Res 30:191-9. 2010
    ..Statins stimulate osteoblast differentiation in vitro and may be a promising drug for the treatment of osteoporosis in the future...
  79. pmc Cds1 controls the release of Cdc14-like phosphatase Flp1 from the nucleolus to drive full activation of the checkpoint response to replication stress in fission yeast
    Helena Díaz-Cuervo
    Instituto de Biologia Molecular y Celular del Cancer, Departamento de Microbiologia y Genetica, Campus Miguel de Unamuno, Universidad de Salamanca Consejo Superior de Investigaciones Cientificas, 37007 Salamanca, Spain
    Mol Biol Cell 19:2488-99. 2008
    ..Together, our data show that by releasing Flp1p into the nucleus Cds1p checkpoint kinase modulates its own full activation during replication stress...
  80. ncbi BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast
    H O Park
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448
    Nature 365:269-74. 1993
    ..Chromosomal deletion of BUD2 causes a random budding pattern but no obvious growth defect. Overexpression of BUD2 also causes a random budding pattern by wild-type cells...
  81. ncbi The novel HECT-type ubiquitin-protein ligase Pub2p shares partially overlapping function with Pub1p in Schizosaccharomyces pombe
    Katsuyuki K Tamai
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto 3 3 138, Sumiyoshi ku, Osaka 558 8585, Japan
    J Cell Sci 115:1847-57. 2002
    ..We conclude that Pub2p is a HECT-type ubiquitin-protein ligase that shares partially overlapping function with Pub1p...
  82. pmc Transgenerational rescue of a genetic defect in long-term potentiation and memory formation by juvenile enrichment
    Junko A Arai
    Sackler School of Biomedical Sciences and Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Neurosci 29:1496-502. 2009
    ....
  83. ncbi CAPRI regulates Ca(2+)-dependent inactivation of the Ras-MAPK pathway
    P J Lockyer
    Department of Biochemistry, School of Medical Sciences, University of Bristol, BS8 1TD, Bristol, United Kingdom
    Curr Biol 11:981-6. 2001
    ..Analysis of the spatio-temporal dynamics of CAPRI indicates that Ca(2+) regulates the GAP by a fast C2 domain-dependent translocation mechanism...
  84. ncbi Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins
    A M Chan
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland, MD 20892
    Oncogene 9:1057-63. 1994
    ..The 6.5 kilobasepair (kb) transcript of the TIM gene was mainly expressed in kidney, liver, pancreas, lung, and placenta. By analysing a panel of human-hamster somatic cell hybrids, we localized the TIM gene to human chromosome 7...
  85. ncbi Neural cell adhesion molecule-associated polysialic acid regulates synaptic plasticity and learning by restraining the signaling through GluN2B-containing NMDA receptors
    Gaga Kochlamazashvili
    Zentrum fur Molekulare Neurobiologie Hamburg, University Medical Center Hamburg Eppendorf, 20246 Hamburg, Germany
    J Neurosci 30:4171-83. 2010
    ....
  86. pmc DNA sequence polymorphisms in a panel of eight candidate bovine imprinted genes and their association with performance traits in Irish Holstein-Friesian cattle
    David A Magee
    Animal Genomics Laboratory, UCD School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
    BMC Genet 11:93. 2010
    ..e. human/mouse/pig/sheep)...
  87. ncbi Modulation of the Ras/Raf/MEK/ERK pathway by Ca(2+), and calmodulin
    Neus Agell
    Departament de Biologia Cel lular i Anatomia Patològica, Institut d Investigacions Biomediques August Pi i Sunyer, Facultat de Medicina Universitat de Barcelona, c Casanova, 143, 08036 Barcelona, Spain
    Cell Signal 14:649-54. 2002
    ..In fibroblasts, CaM binding to EGF receptor and K-Ras(B) may be involved in the downregulation of the pathway after its activation, allowing a proliferative signalling...
  88. ncbi A Candida albicans homolog of CDC25 is functional in Saccharomyces cerevisiae
    D Goldberg
    Department of Biological Chemistry, Hebrew University of Jerusalem, Israel
    Eur J Biochem 213:195-204. 1993
    ..cerevisiae. In addition to the full length protein (approximately 150 kDa), we have found a approximately 50-kDa polypeptide which seems to include the C-terminus of the CSC25 gene product...
  89. pmc Identification of cell cycle-regulated genes in fission yeast
    Xu Peng
    Genome Institute of Singapore, Singapore 138672, Singapore
    Mol Biol Cell 16:1026-42. 2005
    ..Our complete data sets are available at http://giscompute.gis.a-star.edu.sg/~gisljh/CDC...
  90. ncbi SIN and the art of splitting the fission yeast cell
    Andrea Krapp
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges, Switzerland
    Curr Biol 14:R722-30. 2004
    ..Flp1p, the S. pombe ortholog of Cdc14p, is not essential for mitotic exit, but may contribute to an orderly mitosis-G1 transition by regulating the destruction of the mitotic inducer cdc25p...
  91. ncbi Tobacco BY-2 cells expressing fission yeast cdc25 bypass a G2/M block on the cell cycle
    Craig B Orchard
    School of Biosciences, Cardiff University, PO Box 915, Cardiff CF10 3TL, UK
    Plant J 44:290-9. 2005
    ..In conclusion, synchronised Spcdc25-expressing BY-2 cells divided prematurely at a small cell size, and they exhibited premature, but sustained, CDKB1 activity even though endogenous cytokinins were virtually undetectable...
  92. pmc Schizosaccharomyces pombe Dss1p is a DNA damage checkpoint protein that recruits Rad24p, Cdc25p, and Rae1p to DNA double-strand breaks
    Saravana P Selvanathan
    Basic Research Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 285:14122-33. 2010
    ..We suggest that the sequestration of Cdc25p to DNA damage sites could provide a mechanism for S. pombe cells to arrest at G(2)/M boundary in response to DNA damage...
  93. pmc Activation of Srk1 by the mitogen-activated protein kinase Sty1/Spc1 precedes its dissociation from the kinase and signals its degradation
    Sandra López-Avilés
    Departament de Biologia Cellular, Universitat de Barcelona Institut d Investigacions Biomèdiques August Pi i Sunyer, 08036 Barcelona, Catalunya, Spain
    Mol Biol Cell 19:1670-9. 2008
    ....
  94. ncbi stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe
    J D Hudson
    Department of Biology, Queen s University, Kingston, Ontario, Canada
    Cold Spring Harb Symp Quant Biol 56:599-604. 1991
    ..It does not appear to play a role in the nutritional sensing pathway nor in the pathway mediating radiation-induced G2 delay...
  95. ncbi The fission yeast mitotic activator cdc25 and sucrose induce early flowering synergistically in the day-neutral Nicotiana tabacum cv. Samsun
    Martina Teichmanová
    Department of Plant Physiology, Faculty of Science, Charles University, Vinicna 5, 128 44, Prague 2, Czech Republic
    New Phytol 176:804-12. 2007
    ..Our data demonstrate that Spcdc25 expression and sucrose act synergistically to induce precocious flowering...
  96. ncbi A novel Dictyostelium RasGEF is required for normal endocytosis, cell motility and multicellular development
    A Wilkins
    MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, WC1E 6BT, London, UK
    Curr Biol 10:1427-37. 2000
    ..It is also unclear, in Dictyostelium or in higher eukaryotes, whether Ras pathways are linear, with each Ras controlled by its own GEF, or networked, with multiple GEFs acting on multiple Ras proteins...
  97. ncbi Different kinetic properties of the two mutants, RAS2Ile152 and RAS2Val19, that suppress the CDC25 requirement in RAS/adenylate cyclase pathway in Saccharomyces cerevisiae
    J B Crechet
    Laboratoire de Biochimie, URA 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France
    J Biol Chem 265:1563-8. 1990
    ..This stresses the importance of a rapid regeneration of the RAS2.GTP complex for the activation of the adenylate cyclase pathway...
  98. ncbi Human low molecular weight neurofilament (NFL) mRNA interacts with a predicted p190RhoGEF homologue (RGNEF) in humans
    Kathryn Volkening
    Robarts Research Institute, London, Ontario, Canada
    Amyotroph Lateral Scler 11:97-103. 2010
    ....
  99. pmc Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) controls activation of extracellular signal-regulated kinase (ERK) signaling in the striatum and long-term behavioral responses to cocaine
    Stefania Fasano
    Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Foundation and University, Milano, Italy
    Biol Psychiatry 66:758-68. 2009
    ....
  100. ncbi Laser microdissection and microarray analysis of the hippocampus of Ras-GRF1 knockout mice reveals gene expression changes affecting signal transduction pathways related to memory and learning
    A Fernandez-Medarde
    Centro de Investigacion del Cancer, IBMCC CSIC USAL, Campus Unamuno, University of Salamanca, 37007 Salamanca, Spain
    Neuroscience 146:272-85. 2007
    ..These data, together with the previously described role of IRS and insulin (known Ras-GRF1 activators) in AD, warrant further investigation of a potential functional link of Ras-GRF1 to neurodegenerative processes...
  101. ncbi Structure-activity studies on arylamides and arysulfonamides Ras inhibitors
    Sonia Colombo
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    Curr Cancer Drug Targets 10:192-9. 2010
    ..In the case both these moieties, the hydroxylamine and sulfonamide are absent, inactive compounds are obtained...

Research Grants15

  1. Epigenetic Consequences of Prenatal Alcohol Exposure
    Eva E Redei; Fiscal Year: 2013
    ..Identifying the mechanisms and potential reversibility of transgenerational effects and devising novel interventions in the animal model of FAE is a major goal that could potentially be beneficial for human FAE. ..
  2. Epigenetic Control of a Ras Activator
    Paul D Soloway; Fiscal Year: 2013
    ..Such studies are fundamental to understand how epigenetic states are controlled and will identify critical points of regulation where this control can go awry in human disease. ..
  3. GENETIC ANALYSIS OF RAS AND G PROTEIN FUNCTION
    LARRY FEIG; Fiscal Year: 2007
    ..These studies will exploit our recent finding that constitutively activated R-Ras is a potent activator of estrogen independent proliferation in MCF-7 breast cancer cell lines. ..
  4. ONCOGENESIS/CONTROL--PHOSPHOINOSITIDE CYCLE/KINASE C
    Ian Macara; Fiscal Year: 2001
    ..Overall these studies should lead to significant new insights into signaling through the Ras pathway, and may lead to new therapeutic targets for certain types of cancer. ..
  5. Matrix metalloproteinase-9 mediated cell death in stroke
    ENG LO; Fiscal Year: 2009
    ..Our lab and others have established MMP-9 as a key extracellular protease that modifies neurovascular homeostasis. These proposed studies should help reveal the mechanisms of MMP-9 mediated cell death in stroke. ..
  6. Regulation of Ras through the Ras GRF exchange factor
    Raymond Mattingly; Fiscal Year: 2005
    ..The results are likely to be of fundamental importance to our comprehension of the role of the Ras/MAP kinase system in learning and memory. ..
  7. CELLULAR AND MOLECULAR BASIS OF HIV BASED THROMBOCYTOPEN
    Mariusz Ratajczak; Fiscal Year: 2002
    ..In toto, the studies proposed in this grant will increase the knowledge about pathogenesis of AIDS associated thrombocytopenia and may lead to development of new strategies for its treatment of prevention. (End of Abstract) ..
  8. The CXCR4-SDF-1 Axis in Metastatic Rhabdomyosarcoma
    Mariusz Ratajczak; Fiscal Year: 2009
    ..This work will be of relevance for designing strategies to block the SDF-1-CXCR4 axis to treat CXCR4-positive cancers. ..
  9. role of BLyS/April in Normal and Malignant Plasma Cells
    Diane Jelinek; Fiscal Year: 2008
    ..abstract_text> ..
  10. Molecular Epidemiology of Pancreatic Cancer
    Donghui Li; Fiscal Year: 2008
    ..Such information will be valuable for the primary prevention of this deadly disease. ..
  11. MAP Kinase and AKT Signaling in Congestive Heart Failure
    ANTHONY MUSLIN; Fiscal Year: 2009
    ..Our findings will help to identify specific signaling mechanisms involved in the regulation of cardiac myocyte growth and metabolism. ..
  12. RGS PROTEIN FUNCTION IN CARDIAC PHYSIOLOGY
    ANTHONY MUSLIN; Fiscal Year: 2008
    ..These experiments will help define the role of RGS proteins in the pathogenesis of cardiac hypertrophy and contractile dysfunction and may provide important information for the development of novel therapeutic agents. ..
  13. MOLECULAR AND BIOLOGICAL FUNCTION OF CD40 IN MYELOMA
    Diane Jelinek; Fiscal Year: 2002
    ....
  14. 14-3-3 PROTEIN FUNCTION IN CELL GROWTH AND MOTILITY
    ANTHONY MUSLIN; Fiscal Year: 2001
    ..The expectation is that the results of these experiments will establish the importance of the 14-3-3 signaling molecules in cell growth regulation and development. ..
  15. Rheb GTPase as chemotherapeutic target for brain tumors
    LAWRENCE QUILLIAM; Fiscal Year: 2009
    ..They will address the molecular basis of non-Ras action of FTIs and demonstrate the value of targeting Rheb in cancer therapy. ..