guanine nucleotide dissociation inhibitors

Summary

Summary: Protein factors that inhibit the dissociation of GDP from GTP-BINDING PROTEINS.

Top Publications

  1. pmc Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1
    Etienne Boulter
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 12:477-83. 2010
  2. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001
  3. ncbi RabGDI displacement by DrrA from Legionella is a consequence of its guanine nucleotide exchange activity
    Stefan Schoebel
    Department of Physical Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, NRW, Germany
    Mol Cell 36:1060-72. 2009
  4. pmc Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity
    Arupratan Das
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Nat Cell Biol 14:304-10. 2012
  5. ncbi The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI
    Toshihide Yamashita
    Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nat Neurosci 6:461-7. 2003
  6. pmc Dual modes of cdc42 recycling fine-tune polarized morphogenesis
    Brian D Slaughter
    Stowers Institute for Medical Research, 1000 East 50 th Street, Kansas City, MO 64110, USA
    Dev Cell 17:823-35. 2009
  7. pmc Dissociation of Rac1(GDP).RhoGDI complexes by the cooperative action of anionic liposomes containing phosphatidylinositol 3,4,5-trisphosphate, Rac guanine nucleotide exchange factor, and GTP
    Yelena Ugolev
    Julius Friedrich Cohnheim Minerva Center for Phagocyte Research and the Ela Kodesz Institute of Host Defense against Infectious Diseases, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 283:22257-71. 2008
  8. ncbi Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI
    G R Hoffman
    Department of Molecular Medicine, Veterinary Medical Center, Cornell University, Ithaca, New York 14853, USA
    Cell 100:345-56. 2000
  9. ncbi Microtubule-induced Pins/Galphai cortical polarity in Drosophila neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Cell 123:1323-35. 2005
  10. ncbi Rho GDP dissociation inhibitor protects cancer cells against drug-induced apoptosis
    Baolin Zhang
    Laboratory of Biochemistry, Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Cancer Res 65:6054-62. 2005

Research Grants

  1. RhoGDI: yin and yang of RhoGTPases cycle
    Celine DerMardirossian; Fiscal Year: 2013
  2. Modulation of G proteins by Growth Factors
    Pradipta Ghosh; Fiscal Year: 2013
  3. INTERACTION OF RHO GTPASES WITH REGULATORS AND EFFECTORS
    Yi Zheng; Fiscal Year: 2007
  4. Mechanistic studies of a novel G-alpha nucleotide cycle
    DAVID SIDEROVSKI; Fiscal Year: 2009
  5. Allosteric, Small-Molecule Inhibitors of Actin Nucleation by the Formin mDia1
    Jeffrey Peterson; Fiscal Year: 2008
  6. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Celine DerMardirossian; Fiscal Year: 2010
  7. Molecular Analysis of the CCC185 Golgin
    Suzanne R Pfeffer; Fiscal Year: 2010
  8. Dynamic Analysis of Rho GTPase-Rho GDI Cycling
    Leslie M Loew; Fiscal Year: 2010
  9. Cofilin/ADF Regulation in Rho GTPase Signaling
    Celine DerMardirossian; Fiscal Year: 2010
  10. G-protein signal coordination by RGS12
    DAVID SIDEROVSKI; Fiscal Year: 2009

Detail Information

Publications207 found, 100 shown here

  1. pmc Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1
    Etienne Boulter
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 12:477-83. 2010
    At steady state, most Rho GTPases are bound in the cytosol to Rho guanine nucleotide dissociation inhibitors (RhoGDIs). RhoGDIs have generally been considered to hold Rho proteins passively in an inactive state within the cytoplasm...
  2. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001
    ..Thus, despite the common essential feature of the CAAX (prenylation, AAX tripeptide proteolysis, and carboxyl methylation) motif, the subcellular localizations of Rho GTPases, like their functions, are diverse and dynamic...
  3. ncbi RabGDI displacement by DrrA from Legionella is a consequence of its guanine nucleotide exchange activity
    Stefan Schoebel
    Department of Physical Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, NRW, Germany
    Mol Cell 36:1060-72. 2009
    ..Thus, apparent GDI displacement by DrrA is linked directly to nucleotide exchange, suggesting a basic model for GDI displacement and specificity of Rab localization that does not require discrete GDI displacement activity...
  4. pmc Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity
    Arupratan Das
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Nat Cell Biol 14:304-10. 2012
    ....
  5. ncbi The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI
    Toshihide Yamashita
    Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nat Neurosci 6:461-7. 2003
    ..This peptide has potential as a therapeutic agent against the inhibitory cues that block regeneration in the central nervous system...
  6. pmc Dual modes of cdc42 recycling fine-tune polarized morphogenesis
    Brian D Slaughter
    Stowers Institute for Medical Research, 1000 East 50 th Street, Kansas City, MO 64110, USA
    Dev Cell 17:823-35. 2009
    ....
  7. pmc Dissociation of Rac1(GDP).RhoGDI complexes by the cooperative action of anionic liposomes containing phosphatidylinositol 3,4,5-trisphosphate, Rac guanine nucleotide exchange factor, and GTP
    Yelena Ugolev
    Julius Friedrich Cohnheim Minerva Center for Phagocyte Research and the Ela Kodesz Institute of Host Defense against Infectious Diseases, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 283:22257-71. 2008
    ..RhoGDI complexes, p67(phox), GTP, and Rac GEF constructs with affinity for PtdIns(3,4,5)P(3) at a level superior to that of native membranes...
  8. ncbi Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI
    G R Hoffman
    Department of Molecular Medicine, Veterinary Medical Center, Cornell University, Ithaca, New York 14853, USA
    Cell 100:345-56. 2000
    ..The structural data demonstrate how GDIs serve as negative regulators of small GTP-binding proteins and how the isoprenoid moiety is utilized in this critical regulatory interaction...
  9. ncbi Microtubule-induced Pins/Galphai cortical polarity in Drosophila neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Cell 123:1323-35. 2005
    ..These results identify a role for microtubules in polarizing the neuroblast cortex, a fundamental step for generating cell diversity through asymmetric cell division...
  10. ncbi Rho GDP dissociation inhibitor protects cancer cells against drug-induced apoptosis
    Baolin Zhang
    Laboratory of Biochemistry, Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Cancer Res 65:6054-62. 2005
    ..Taken together, the data show that RhoGDI is an anti-apoptotic molecule that mediates cellular resistance to these chemotherapy agents...
  11. ncbi RhoGDI2 is an invasion and metastasis suppressor gene in human cancer
    John J Gildea
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Cancer Res 62:6418-23. 2002
    ..These results indicate that RhoGDI2 is a metastasis suppressor gene, a marker of aggressive human cancer, and a promising target for therapy...
  12. ncbi Controlling the location and activation of Rab GTPases
    Miguel C Seabra
    Cell and Molecular Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, UK
    Curr Opin Cell Biol 16:451-7. 2004
    ..The diversity of cellular processes regulated by Rab family members is made possible, not only by the wide range of effectors they recruit, but also by the different mechanisms regulating their own targeting and activation...
  13. ncbi RhoGDI2 expression is associated with tumor growth and malignant progression of gastric cancer
    Hee Jun Cho
    Department of Microbiology Research Institute of Life Science, College of Natural Sciences, Physiology, Anatomy and Neurobiology, Institute of Health Science, Gyeongsang National University, Jinju, Korea
    Clin Cancer Res 15:2612-9. 2009
    ..Here, we assessed the effects of RhoGDI2 expression on gastric tumor growth and metastasis progression...
  14. ncbi Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein
    K Takahashi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
    J Biol Chem 272:23371-5. 1997
    ..These results suggest that ERM as well as Rho GDI and the Rho GDP/GTP exchange factor are involved in the activation of the Rho subfamily members, which then regulate reorganization of actin filaments through the ERM system...
  15. ncbi Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae
    G Koch
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    Oncogene 15:417-22. 1997
    ..These results indicate that yeast Rho GDI possesses properties similar to those of mammalian Rho GDI, and that there is a cytosolic factor which functionally substitutes for Rho GDI in yeast...
  16. pmc RhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas
    C N Adra
    Harvard Institutes of Medicine, HIM955, Beth Israel Hospital, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 94:4279-84. 1997
    ..We propose that RhoGDI be renamed RhoGDIalpha and GDID4 be renamed RhoGDIbeta. The new GDI should widen the scope of investigation of this important class of regulatory protein...
  17. ncbi Rho guanine dissociation inhibitors: pivotal molecules in cellular signalling
    B Olofsson
    CNRS UPR 9063, Laboratoire d Enzymologie et Biochimie Structurales, Gif sur Yvette, France
    Cell Signal 11:545-54. 1999
    ..will describe one family of crucial regulators acting on proteins in the Rho/Rac family-the Rho guanine nucleotide dissociation inhibitors, or RhoGDIs...
  18. pmc Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops
    Tina Freisinger
    Max Planck Institute of Biochemistry, Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Commun 4:1807. 2013
    ..Our results demonstrate how cells optimize symmetry breaking through coupling between multiple feedback loops...
  19. ncbi The GDI-like solubilizing factor PDEδ sustains the spatial organization and signalling of Ras family proteins
    Anchal Chandra
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto Hahn Str 11, 44227 Dortmund, Germany
    Nat Cell Biol 14:148-58. 2012
    ..Our findings link the activity of PDEδ in determining Ras protein topography to Ras-dependent signalling...
  20. ncbi Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton
    H Leffers
    Institute of Medical Biochemistry, Aarhus University, Denmark
    Exp Cell Res 209:165-74. 1993
    ..Taken together, the results imply that Rho GDI's play a role in modulating the activity of the Rho proteins as their overexpression mimics phenotypic changes associated with the inactivation of these proteins...
  21. ncbi Overexpression of Rho GDP-dissociation inhibitor alpha is associated with tumor progression and poor prognosis of colorectal cancer
    Liang Zhao
    Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China
    J Proteome Res 7:3994-4003. 2008
    ..These data suggest that RhoGDI may promote CRC progression and metastasis by stimulating tumor cell growth and migration...
  22. pmc Sequential action of two GTPases to promote vacuole docking and fusion
    G Eitzen
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    EMBO J 19:6713-20. 2000
    ..Thus, two GTPases sequentially govern vacuole docking and fusion...
  23. ncbi Calcium signaling regulates translocation and activation of Rac
    Leo S Price
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands
    J Biol Chem 278:39413-21. 2003
    ..Intracellular calcium signaling may thus contribute to the intracellular localization and activation of Rac to regulate the cytoskeletal changes in response to receptor stimulation...
  24. pmc Canoe binds RanGTP to promote Pins(TPR)/Mud-mediated spindle orientation
    Brett Wee
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    J Cell Biol 195:369-76. 2011
    ..We show that the Canoe Ras-association (RA) domains directly bind RanGTP and that both the Canoe(RA) domains and RanGTP are required to recruit Mud to the cortex and activate the Pins/Mud/dynein spindle orientation pathway...
  25. ncbi RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos
    Katayoun Afshar
    Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges Lausanne, Switzerland
    Cell 119:219-30. 2004
    ..Our findings suggest a mechanism in which RIC-8 favors generation of Galpha free from Gbetagamma and enables GPR-1/2 to mediate asymmetric cell division...
  26. pmc The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex
    Tracy J LaGrassa
    Biochemie Zentrum der Universitat Heidelberg, 69120 Heidelberg, Germany
    J Cell Biol 168:401-14. 2005
    ..Because Yck3 also promotes efficient vacuole inheritance, we propose that tethering complex phosphorylation is a part of a general, switch-like mechanism for driving changes in organelle architecture...
  27. pmc New insights into how the Rho guanine nucleotide dissociation inhibitor regulates the interaction of Cdc42 with membranes
    Jared L Johnson
    Department of Chemistry, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 284:23860-71. 2009
    ....
  28. ncbi The Drosophila NuMA Homolog Mud regulates spindle orientation in asymmetric cell division
    Sarah K Bowman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Dev Cell 10:731-42. 2006
    ..Our data suggest a model in which asymmetrically localized Pins-G alphai complexes regulate spindle orientation by directly binding to Mud...
  29. pmc Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells
    Christopher A Johnston
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell 138:1150-63. 2009
    ..This "induced cortical polarity" assay is suitable for rapid identification of the proteins, domains, and amino acids regulating spindle orientation or cell polarity...
  30. ncbi Rho GTPases are over-expressed in human tumors
    G Fritz
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Germany
    Int J Cancer 81:682-7. 1999
    ..Overall, increase in the amount of Rho GTPases, in particular RhoA, appears to be a frequent event in different types of human tumors. This supports the view that Rho GTPases are involved in human carcinogenesis...
  31. ncbi Reduced expression of metastasis suppressor RhoGDI2 is associated with decreased survival for patients with bladder cancer
    Dan Theodorescu
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Clin Cancer Res 10:3800-6. 2004
    ....
  32. ncbi The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts
    Karsten H Siller
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
    Nat Cell Biol 8:594-600. 2006
    ..We propose that Mud is a functional orthologue of mammalian NuMA and Caenorhabditis elegans Lin-5, and that Mud coordinates spindle orientation with cortical polarity to promote asymmetric cell division...
  33. ncbi D4-GDI, a Rho GTPase regulator, promotes breast cancer cell invasiveness
    Yaqin Zhang
    Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Cancer Res 66:5592-8. 2006
    ..Knockdown of D4-GDI in BT549 cells results in a similar effect. These results show that D4-GDI modulates breast cancer cell invasive activities...
  34. ncbi A Rho GDP-dissociation inhibitor is involved in cytokinesis of Dictyostelium
    Keita Imai
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    Biochem Biophys Res Commun 296:305-12. 2002
    ..These results suggest that RhoGDI1 is involved in cytokinesis of Dicytostelium through the regulation of Rho family GTPases Rac1s and/or RacE...
  35. pmc Differential phosphorylation of RhoGDI mediates the distinct cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion
    Zhanxiang Wang
    Herman B Wells Center for Pediatric Research, Basic Diabetes Research Group, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Biol Chem 285:6186-97. 2010
    ....
  36. pmc Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts
    Rick W Nipper
    Institute of Molecular Biology, Department of Chemistry, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 104:14306-11. 2007
    ..We conclude that Pins has multiple activity states that generate cortical polarity and link it with mitotic spindle orientation...
  37. pmc Ly-GDI, a GDP-dissociation inhibitor of the RhoA GTP-binding protein, is expressed preferentially in lymphocytes
    P Scherle
    Metabolism Branch, National Cancer Institute, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 90:7568-72. 1993
    ....
  38. pmc A novel role for RhoGDI as an inhibitor of GAP proteins
    J F Hancock
    Onyx Pharmaceuticals, Richmond, CA 94806
    EMBO J 12:1915-21. 1993
    ..Thus GDI, by complexing with Rac-GTP and preventing GAP stimulated GTP hydrolysis, may allow transit of the activated form of the Rac protein between physically separated activator and effector proteins in the cell...
  39. ncbi GDIs: central regulatory molecules in Rho GTPase activation
    Celine DerMardirossian
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Cell Biol 15:356-63. 2005
    ..We propose that phosphorylation mediated by diverse kinases might provide a means of controlling and coordinating Rho GTPase activation...
  40. ncbi Rho GTPase signaling in Dictyostelium discoideum: insights from the genome
    Georgia Vlahou
    Center for Biochemistry of the Medical Faculty and Center for Molecular Medicine Cologne, University of Cologne, Joseph Stelzmann Str 52, D 50931 Cologne, Germany
    Eur J Cell Biol 85:947-59. 2006
    ..In general, the repertoire of Rho signaling components of Dictyostelium is similar to that of metazoa and fungi...
  41. ncbi Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division
    Katayoun Afshar
    Swiss Institute for Experimental Cancer Research ISREC Institute of Technology EPFL, CH 1066 Lausanne, Switzerland
    Development 132:4449-59. 2005
    ....
  42. ncbi Rho GTPases as modulators of the estrogen receptor transcriptional response
    L F Su
    Department of Microbiology and the Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 276:3231-7. 2001
    ..Since Rho GTPases regulate actin polymerization, our findings suggest a link between the major regulators of cellular architecture and steroid receptor transcriptional response...
  43. ncbi The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI
    K Scheffzek
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Otto Hahn Str 11, 44227 Dortmund, Germany
    Nat Struct Biol 7:122-6. 2000
    Rho family-specific guanine nucleotide dissociation inhibitors (RhoGDIs) decrease the rate of nucleotide dissociation and release Rho proteins such as RhoA, Rac and Cdc42 from membranes, forming tight complexes that shuttle between ..
  44. ncbi The stress-induced MAP kinase p38 regulates endocytic trafficking via the GDI:Rab5 complex
    V Cavalli
    Department of Biochemistry, Sciences II, University of Geneva, 30 Quai Ernest Ansermet, 1211 4, Geneva, Switzerland
    Mol Cell 7:421-32. 2001
    ..Our observations reveal the existence of a cross-talk between endocytosis and the p38-dependent stress response, thus providing molecular evidence that endocytosis can be regulated by the environment...
  45. ncbi Rab GTPases: specifying and deciphering organelle identity and function
    S R Pfeffer
    Dept of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Trends Cell Biol 11:487-91. 2001
    ..Rabs organize distinct protein scaffolds within a single organelle and act in a combinatorial manner with their effectors to regulate all stages of membrane traffic...
  46. ncbi Progressive impairment of kidneys and reproductive organs in mice lacking Rho GDIalpha
    A Togawa
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
    Oncogene 18:5373-80. 1999
    ....
  47. ncbi RhoGDI is required for Cdc42-mediated cellular transformation
    Qiong Lin
    Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA
    Curr Biol 13:1469-79. 2003
    ..In this study, we set out to examine how the regulatory protein RhoGDI influences the cellular responses elicited by activated Cdc42...
  48. ncbi The structural and mechanistic basis for recycling of Rab proteins between membrane compartments
    R S Goody
    Abteilung Physikalische Biochemie, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227, Dortmund, Germany
    Cell Mol Life Sci 62:1657-70. 2005
    ..Here, the structural and mechanistic basis of events occurring in Rab delivery and cycling, and the differences between REP and GDI are discussed on the basis of recent advances in the field...
  49. ncbi Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering
    B D Moyer
    Department of Cell and Molecular Biology, The Institute for Childhood and Neglected Diseases, The Scripps Research Institute, 10550 N Torrey Pines Road, San Diego, California 92037, USA
    Traffic 2:268-76. 2001
    ..We propose a 'homing hypothesis' in which the same Rab interacts with distinct tethering factors at donor and acceptor membranes to program heterotypic membrane fusion events between transport intermediates and their target compartments...
  50. ncbi Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth
    Tamara J Richman
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, 95 Carrigan Dr, 202 Stafford Hall, Burlington, VT 05405, USA
    Curr Genet 45:339-49. 2004
    ..Taken together, these results provide insight into the complicated nature of the relationships between Cdc42p localization, nucleotide binding, and protein-protein interactions...
  51. ncbi Expression profile of RhoGDI2 in lung cancers and role of RhoGDI2 in lung cancer metastasis
    Huiyan Niu
    Department of Geriatrics, Shengjing Hospital, China Medical University, Shenyang 110004, PR China
    Oncol Rep 24:465-71. 2010
    ..Our results suggest that RhoGDI2 is likely to be involved in lung tumor malignancy and metastasis...
  52. pmc An activating mutant of Rac1 that fails to interact with Rho GDP-dissociation inhibitor stimulates membrane ruffling in mammalian cells
    Payal N Gandhi
    Department of Pharmacology, Case Western Reserve University School of Medicine, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Biochem J 378:409-19. 2004
    ..These findings suggest that interaction with RhoGDI is not essential in the mechanism by which Rac1 translocates to the plasma membrane to stimulate ruffle formation...
  53. ncbi Return of the GDI: the GoLoco motif in cell division
    Francis S Willard
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599 7365, USA
    Annu Rev Biochem 73:925-51. 2004
    ..We discuss potential mechanisms by which GoLoco/Galpha complexes might modulate spindle dynamics...
  54. ncbi Capture of the small GTPase Rab5 by GDI: regulation by p38 MAP kinase
    Michela Felberbaum-Corti
    Methods Enzymol 403:367-81. 2005
    ..In addition, we detail how to investigate the possible role of p38 MAPK in the regulation of endocytosis in vivo...
  55. ncbi RhoGDI-3 is a new GDP dissociation inhibitor (GDI). Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG
    G Zalcman
    Unité INSERM 248, Section de Recherche, Institut Curie, 26 Rue d Ulm, 75231 Paris Cedex 05, France
    J Biol Chem 271:30366-74. 1996
    ..No interaction is found with RhoA, RhoC, or Rac1 proteins. We show that GDI-3 is able to inhibit GDP/GTP exchange of RhoB and to release GDP-bound but not GTP-bound RhoB from cell membranes...
  56. pmc RhoGDI-binding-defective mutant of Cdc42Hs targets to membranes and activates filopodia formation but does not cycle with the cytosol of mammalian cells
    R M Gibson
    Department of Pharmacology, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4965, USA
    Biochem J 359:285-94. 2001
    ..However, it does seem that redistribution of Cdc42Hs to the cytosolic compartment is absolutely dependent on RhoGDI interaction...
  57. ncbi Crystal structure of the Rac1-RhoGDI complex involved in nadph oxidase activation
    S Grizot
    Institut de Biologie Structurale, CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Biochemistry 40:10007-13. 2001
    ..In the Rac1-RhoGDI complex, the accessibility of the effector loops of Rac1 probably accounts for the ability of the Rac1-RhoGDI complex to activate the NADPH oxidase...
  58. pmc Morphological and proliferative abnormalities in renal mesangial cells lacking RhoGDI
    Heike Bielek
    Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, United States
    Cell Signal 21:1974-83. 2009
    ..These functional and structural changes are likely to contribute to the defects in renal architecture and function observed in the RhoGDI (-/-) mouse...
  59. ncbi A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm
    N H Keep
    Department of Medicine, University College London, Rayne Institute, 5 University Street, London, WC1E 6JJ, UK
    Structure 5:623-33. 1997
    ..Additionally, rac has a role in the regulation of phagocyte NADPH oxidase. Guanine nucleotide dissociation inhibitors (GDIs) of the rhoGDI family bind to these G proteins and regulate their activity by preventing ..
  60. pmc GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway
    M D Garrett
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510
    EMBO J 13:1718-28. 1994
    ....
  61. ncbi Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase
    Celine DerMardirossian
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 15:117-27. 2004
    ..These results support a role for Pak1-mediated RhoGDI phosphorylation as a mechanism for Cdc42-mediated Rac activation, and suggest the possibility of Rac-induced positive feed-forward regulation of Rac activity...
  62. pmc Structural insights into the dual nucleotide exchange and GDI displacement activity of SidM/DrrA
    Hye Young Suh
    Department of Life Sciences and Center for Biomolecular Recognition, Pohang University of Science and Technology, Pohang, Kyungbuk, Korea
    EMBO J 29:496-504. 2010
    ..In comparison with an eukaryotic GEF TRAPP I, this bacterial GEF/GDF exhibits high binding affinity for Rab1 with GDP retained at the active site, which appears as the key feature for the GDF activity of the protein...
  63. ncbi Membrane targeting of a Rab GTPase that fails to associate with Rab escort protein (REP) or guanine nucleotide dissociation inhibitor (GDI)
    J H Overmeyer
    Department of Biochemistry and Molecular Biology, Medical College of Ohio, Toledo, Ohio 43614-5804, USA
    J Biol Chem 276:20379-86. 2001
    ..Therefore, protein interactions mediated by the alpha2 helical domain of Rab1B(S22N) appear to be essential for its functional interaction with components of the ER --> Golgi transport machinery...
  64. pmc RhoGDI: multiple functions in the regulation of Rho family GTPase activities
    Athanassios Dovas
    Division of Biomedical Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Biochem J 390:1-9. 2005
    ..All these results point to a wider role for RhoGDI than initially perceived, making it a binding partner that can tightly control Rho GTPases, but which also allows them to reach their full spectrum of activities...
  65. pmc X-linked inhibitor of apoptosis protein (XIAP) mediates cancer cell motility via Rho GDP dissociation inhibitor (RhoGDI)-dependent regulation of the cytoskeleton
    Jinyi Liu
    Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, New York 10987, USA
    J Biol Chem 286:15630-40. 2011
    ....
  66. pmc Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif
    Randall J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina, Chapel Hill, NC 27599, USA
    Biochem J 378:801-8. 2004
    ..Galpha(i/o)-Loco' interaction) motif proteins have recently been identified as novel GDIs (guanine nucleotide dissociation inhibitors) for heterotrimeric G-protein alpha subunits...
  67. pmc The Fz-Dsh planar cell polarity pathway induces oriented cell division via Mud/NuMA in Drosophila and zebrafish
    Marion Segalen
    Polarity Division and Morphogenesis, Institut Curie, CNRS UMR 3215, INSERM U934, 75248 Paris Cedex 05, France
    Dev Cell 19:740-52. 2010
    ..Overall, we describe a Frizzled-Dishevelled-NuMA pathway that orients division from Drosophila to vertebrates...
  68. ncbi Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins
    Hongyan Wang
    Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
    Nat Cell Biol 7:1091-8. 2005
    ..In addition, Gbetagamma acts to restrict Galphai (and GoLoco proteins) to the apical cortex, where Galphai (and Pins) can mediate asymmetric spindle geometry...
  69. ncbi RalA-exocyst interaction mediates GTP-dependent exocytosis
    Li Wang
    Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Department of Physiology, University of Toronto, 399 Bathurst Street, Toronto, Ontario M5T 2S8, Canada
    J Biol Chem 279:19875-81. 2004
    ..Furthermore, GTP- and Ca2+-dependent exocytosis use different sensors and effectors for triggering exocytosis whereas their final fusion steps are both SNARE-dependent...
  70. pmc Effects of lovastatin on Rho isoform expression, activity, and association with guanine nucleotide dissociation inhibitors
    Stephanie J Turner
    Department of Medicine and Cancer Center, University of California, San Diego, La Jolla, CA 92093 0652, USA
    Biochem Pharmacol 75:405-13. 2008
    ..In conclusion, we quantified effects of lovastatin on RhoA, B, and C isoforms, and provide a molecular mechanism whereby statins cause accumulation of unprenylated Rho-GTP...
  71. ncbi Molecular cloning and characterization of a novel type of regulatory protein (GDI) for the rho proteins, ras p21-like small GTP-binding proteins
    Y Fukumoto
    Department of Biochemistry, Kobe University School of Medicine, Japan
    Oncogene 5:1321-8. 1990
    ..Computer homology search has revealed that rho GDI is a novel protein that does not share a high amino acid sequence homology with any known protein...
  72. ncbi RhoGDI-1 modulation of the activity of monomeric RhoGTPase RhoA regulates endothelial barrier function in mouse lungs
    Matvey Gorovoy
    University of Illinois College of Medicine, Department of Pharmacology, 835 S Wolcott Ave, Chicago, IL 60612, USA
    Circ Res 101:50-8. 2007
    ..The primary regulators of Rho proteins, guanine nucleotide dissociation inhibitors (GDIs), form a complex with the GDP-bound form of the Rho family of monomeric G proteins, and ..
  73. pmc Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI
    Francisco Rivero
    Institut fur Biochemie I, Medizinische Fakultat, University of Cologne, Joseph Stelzmann Strasse 52, D 50931 Köln, Germany
    EMBO J 21:4539-49. 2002
    ..Our results indicate that GDI1 plays a central role in the regulation of signal transduction cascades mediated by Rho GTPases...
  74. ncbi Molecular dissection of guanine nucleotide dissociation inhibitor function in vivo. Rab-independent binding to membranes and role of Rab recycling factors
    P Luan
    Departments of Cell and Molecular Biology IMM 11, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 274:14806-17. 1999
    ....
  75. pmc Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA
    Yongqun Zhu
    National Institute of Biological Sciences, Beijing 102206, China
    Proc Natl Acad Sci U S A 107:4699-704. 2010
    ..Further biochemical and structural analyses reveal a unique mechanism of coupled GDP release and GDI displacement likely triggered by the SidM-induced drastic displacement of switch I of Rab1...
  76. ncbi Legionella pneumophila proteins that regulate Rab1 membrane cycling
    Alyssa Ingmundson
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA
    Nature 450:365-9. 2007
    ..Thus, L. pneumophila encodes proteins that regulate three distinct biochemical reactions critical for Rab GTPase membrane cycling to redirect Rab1 to the pathogen-occupied vacuole and to control Rab1 function...
  77. ncbi Overcoming expression and purification problems of RhoGDI using a family of "parallel" expression vectors
    P Sheffield
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, 22908, USA
    Protein Expr Purif 15:34-9. 1999
    ....
  78. ncbi Drosophila Pins-binding protein Mud regulates spindle-polarity coupling and centrosome organization
    Yasushi Izumi
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, and CREST, Japan Science and Technology Corporation, 2 2 3 Minatojima minamimachi, Chuou ku, Kobe 650 0047, Japan
    Nat Cell Biol 8:586-93. 2006
    ..We propose that Drosophila Mud, vertebrate NuMA and Caenorhabditis elegans Lin-5 (refs 5, 6) have conserved roles in the mechanism by which G-proteins regulate the mitotic spindle...
  79. ncbi Uncoupling of inhibitory and shuttling functions of rho GDP dissociation inhibitors
    Estelle Dransart
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS UPR 9063, F 91198 Gif sur Yvette, France
    J Biol Chem 280:4674-83. 2005
    ..They should provide valuable tools for future investigations of the dynamics of membrane extraction or delivery of Rho proteins and their regulation by cellular partners...
  80. ncbi The RhoGDI-alpha/JNK signaling pathway plays a significant role in mycophenolic acid-induced apoptosis in an insulin-secreting cell line
    Yun Jong Park
    The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, South Korea
    Cell Signal 21:356-64. 2009
    ..This RhoGDI-alpha/JNK pathway might be the focus of therapeutic target for the prevention of MPA-induced islet apoptosis...
  81. ncbi The PDZ protein Canoe regulates the asymmetric division of Drosophila neuroblasts and muscle progenitors
    Stephan Speicher
    Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Científicas University Miguel Hernandez, Sant Joan d Alacant, 03550 Alicante, Spain
    Curr Biol 18:831-7. 2008
    ....
  82. pmc Membrane targeting of Rab GTPases is influenced by the prenylation motif
    Anita Q Gomes
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, United Kingdom
    Mol Biol Cell 14:1882-99. 2003
    ..Finally, we show that both singly and doubly modified Rabs are extracted from membranes by RabGDIalpha and propose that the mistargeting of Rabs to the ER results from loss of targeting information...
  83. ncbi Molecular cloning and characterization of yeast rho GDP dissociation inhibitor
    T Masuda
    Department of Biochemistry, Kobe University School of Medicine, Japan
    J Biol Chem 269:19713-8. 1994
    ..These results indicate that rho GDI exists and regulates the function of the rho family members in yeast...
  84. ncbi Association of cytosolic Rab4 with GDI isoforms in insulin-sensitive 3T3-L1 adipocytes
    A Shisheva
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 36:6564-70. 1997
    ..Taken together, these data suggest there is selective formation of Rab4-GDI-1 complexes in response to insulin which plays a role in the action of insulin on membrane trafficking...
  85. ncbi C-terminal binding domain of Rho GDP-dissociation inhibitor directs N-terminal inhibitory peptide to GTPases
    Y Q Gosser
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    Nature 387:814-9. 1997
    ..These results lead to a model of rhoGDI function in which the carboxy-terminal binding domain targets the amino-terminal inhibitory region to GTPases, resulting in membrane extraction and inhibition of nucleotide cycling...
  86. ncbi RhoGDI-3 regulates RhoG and targets this protein to the Golgi complex through its unique N-terminal domain
    Nicolas Brunet
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS UPR 9063, F 91198 Gif sur Yvette, France
    Traffic 3:342-57. 2002
    b>Guanine nucleotide dissociation inhibitors (GDIs) regulate both GDP/GTP and membrane association/dissociation cycles of Rho/Rac and Rab proteins...
  87. pmc Suppression of RhoG activity is mediated by a syndecan 4-synectin-RhoGDI1 complex and is reversed by PKCalpha in a Rac1 activation pathway
    Arye Elfenbein
    Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    J Cell Biol 186:75-83. 2009
    ..Thus, FGF2-induced Rac1 activation depends on the suppression of RhoG by a previously uncharacterized ternary S4-synectin-RhoGDI1 protein complex and activation via PKCalpha...
  88. ncbi Rho GDP dissociation inhibitors as potential targets for anticancer treatment
    Baolin Zhang
    Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, 29 Lincoln Dr, Bldg 29A, Rm 2B 24, Bethesda, MD 20892, USA
    Drug Resist Updat 9:134-41. 2006
    ..This review will discuss the dual roles of RhoGDIs in the regulation of Rho GTPases, highlighting a possible role in regulating tumorigenicity. In addition, the potential for targeting RhoGDIs for anticancer therapy will be discussed...
  89. ncbi Immune responses in mice deficient in Ly-GDI, a lymphoid-specific regulator of Rho GTPases
    L Yin
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, U S A
    Mol Immunol 34:481-91. 1997
    ....
  90. ncbi Differential properties of D4/LyGDI versus RhoGDI: phosphorylation and rho GTPase selectivity
    J P Gorvel
    Centre d Immunologie INSERM CNRS de Marseille Luminy, France
    FEBS Lett 422:269-73. 1998
    ..Our results suggest that LyGDI forms complexes with specific rho GTPases expressed in hematopoietic cells where it may regulate specific pathways...
  91. pmc Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway
    M Hirao
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Cell Biol 135:37-51. 1996
    ..These findings indicate that Rho regulates the CD44/ERM complex formation in vivo and that the phosphatidylinositol turnover may be involved in this regulation mechanism...
  92. ncbi Structure and mutational analysis of Rab GDP-dissociation inhibitor
    I Schalk
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 381:42-8. 1996
    ..The two most sequence-conserved regions, which form a compact structure at the apex of GDI, are shown by site-directed mutagenesis to play a critical role in the binding of Rab proteins...
  93. ncbi Inhibition of GDP/GTP exchange on G alpha subunits by proteins containing G-protein regulatory motifs
    M Natochin
    Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    Biochemistry 40:5322-8. 2001
    ..Our results suggest that GPR proteins are potent GDP dissociation inhibitors for Gialpha-like Galpha subunits in vitro, and in this capacity they may inhibit GPCR/Gi protein signaling in vivo...
  94. ncbi Vav1 and Ly-GDI two regulators of Rho GTPases, function cooperatively as signal transducers in T cell antigen receptor-induced pathways
    Maya Groysman
    Hubert H Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Biol Chem 277:50121-30. 2002
    ..Thus, our data suggest that the interaction of Vav1 and Ly-GDI creates a fine tuning mechanism for the regulation of intracellular signaling pathways leading to NFAT stimulation...
  95. pmc Cognitive impairment in Gdi1-deficient mice is associated with altered synaptic vesicle pools and short-term synaptic plasticity, and can be corrected by appropriate learning training
    Veronica Bianchi
    Dulbecco Telethon Institute at DIBIT San Raffaele Scientific Institute, Milan, Italy
    Hum Mol Genet 18:105-17. 2009
    ..This implies that an intact RP of SVs is necessary for memory processing under challenging conditions in mice. The possibility to correct the learning deficit in mice may have clinical implication for future studies in human...
  96. ncbi Interactions between Rho GTPases and Rho GDP dissociation inhibitor (Rho-GDI)
    J Faure
    Laboratoire de Biochimie et Biophysique des Systèmes Intégrés BBSI, UMR CEA CNRS UJF 5092, CEA Grenoble, 17, rue des Martyrs, 38054 Cedex 9, Grenoble, France
    Biochimie 83:409-14. 2001
    ..The C-terminal polybasic region of RhoA was also shown to be a site of protein-protein interaction with Rho-GDI...
  97. ncbi RhoGDIs revisited: novel roles in Rho regulation
    Estelle Dransart
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Traffic 6:957-66. 2005
    ..factors and GTPase-activating proteins, to a cytosol/membrane cycle, regulated by guanine nucleotide dissociation inhibitors (rhoGDIs)...
  98. pmc Cloning, characterization, and expression of a novel GDP dissociation inhibitor isoform from skeletal muscle
    A Shisheva
    Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605
    Mol Cell Biol 14:3459-68. 1994
    ..Taken together, these data demonstrate that the protein products of at least two genes regulate the membrane dynamics of Rab proteins in mice...
  99. pmc In vivo dynamics of Rac-membrane interactions
    Konstadinos Moissoglu
    Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Mol Biol Cell 17:2770-9. 2006
    ..These results indicate that RhoGDI controls Rac function mainly through effects on activation and/or membrane association...
  100. pmc Phosphorylation of RhoGDI by Src regulates Rho GTPase binding and cytosol-membrane cycling
    Celine DerMardirossian
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 17:4760-8. 2006
    ..These results suggest that Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane-cycling and activity...
  101. ncbi Mutations in GDI1 are responsible for X-linked non-specific mental retardation
    P d'Adamo
    Institute of Genetics Biochemistry and Evolution, CNR, Pavia, Italy
    Nat Genet 19:134-9. 1998
    ....

Research Grants22

  1. RhoGDI: yin and yang of RhoGTPases cycle
    Celine DerMardirossian; Fiscal Year: 2013
    ..These studies will lead to a greater understanding of how these important components of renal function are controlled, ultimately guiding to unique and increasingly selective therapeutic approaches to intervene in nephritic disorders. ..
  2. Modulation of G proteins by Growth Factors
    Pradipta Ghosh; Fiscal Year: 2013
    ....
  3. INTERACTION OF RHO GTPASES WITH REGULATORS AND EFFECTORS
    Yi Zheng; Fiscal Year: 2007
    ..e. the guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and the guanine nucleotide dissociation inhibitors (GDIs), are involved in regulating their cellular activities...
  4. Mechanistic studies of a novel G-alpha nucleotide cycle
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ....
  5. Allosteric, Small-Molecule Inhibitors of Actin Nucleation by the Formin mDia1
    Jeffrey Peterson; Fiscal Year: 2008
    ..Future work will apply this screen to a much larger compound collection and we envisage applying a similar strategy to develop inhibitors for other formins. [unreadable] [unreadable] [unreadable]..
  6. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Celine DerMardirossian; Fiscal Year: 2010
    ..We will study how a newly identified group of Src target proteins act to organize Nox assembly and localized activity. Understanding Nox regulation will have important implications for disease therapy. ..
  7. Molecular Analysis of the CCC185 Golgin
    Suzanne R Pfeffer; Fiscal Year: 2010
    ..This work has broad implications for our understanding of vesicle docking and fusion events within the secretory and endocytic pathways that are essential for normal human health and disease. ..
  8. Dynamic Analysis of Rho GTPase-Rho GDI Cycling
    Leslie M Loew; Fiscal Year: 2010
    ..With our studies, we hope to provide a quantitatively detailed, predictive model that can be used to investigate Rho GTPase function in health and disease. ..
  9. Cofilin/ADF Regulation in Rho GTPase Signaling
    Celine DerMardirossian; Fiscal Year: 2010
    ..Our studies will investigate a novel regulator of this important process. In addition, this protein is likely to play important roles in ischemic disorders and other stress responses. ..
  10. G-protein signal coordination by RGS12
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ..We wish to study one RGS protein, RGS12, that interferes with the actions of the neurotransmitter GABA in neurons that control pain processing. This work should lead to the discovery of new pain-controlling drugs. ..
  11. INTRACELLULAR TRANSPORT--THE MANNOSE-PHOSPHATE RECEPTOR
    Suzanne Pfeffer; Fiscal Year: 2008
    ..These studies have important implications for our understanding of growth control and antigen processing, and will provide fundamental information regarding the mechanism of receptor trafficking in mammalian cells. ..
  12. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ....
  13. Signaling Diversity Among Gqa Family Members
    JOHN HEPLER; Fiscal Year: 2004
    ..Information gained from these studies will help us to better understand G proteins as therapeutic targets. ..
  14. LOCALIZATION OF THE GOLGI COMPLEX
    Suzanne Pfeffer; Fiscal Year: 2001
    ....
  15. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
    ..Funds are requested to further test the general applicability and success rate of this method, and to apply the method to a subset of targets selected for the Structural Genomic Initiative. ..
  16. Regulation of exocrine cell secretion by Rab proteins
    Robert Raffaniello; Fiscal Year: 2006
    ..Therefore, an understanding of the basic mechanisms that regulate exocrine gland secretion is essential. [unreadable] [unreadable]..
  17. RGS Protein Regulation of G Protein Coupled Receptors
    John R Hepler; Fiscal Year: 2010
    ..These studies will define novel cellular mechanisms for regulating neurotransmitter and hormone signaling, and identify potential new molecular targets for therapeutic intervention in cardiovascular diseases. ..
  18. Preparing high resolution membrane protein crystals
    Zygmunt Derewenda; Fiscal Year: 2005
    ..abstract_text> ..
  19. Protein Prenyltransferases in Glucose-Stimulated Insulin Secretion
    Anjaneyulu Kowluru; Fiscal Year: 2010
    ....
  20. Role of Rho Kinase in Aqueous Humor Outflow Pathway
    P Rao; Fiscal Year: 2004
    ..Identification of specific signaling mechanisms regulating function of outflow pathway tissues could enable the design of target-specific approaches to the treatment of glaucoma. ..
  21. Real-Time Fluorescence Assay:RGS Domain GAP Activit(RMI)
    DAVID SIDEROVSKI; Fiscal Year: 2008
    ..unreadable]PERFORMANCE SITE ========================================Section End=========================================== ..
  22. ENDOTHELIAL GROWTH FACTOR IN PROSTATE CANCER METASTASIS
    Dan Theodorescu; Fiscal Year: 2005
    ..abstract_text> ..